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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Computing 15 (1975), S. 287-290 
    ISSN: 1436-5057
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-055X
    Keywords: Schlüsselwörter EEG ; Anästhesie ; Monitoring ; Ableitungsauswahl ; automatische Narkosetiefebestimmung ; Key words EEG ; Anaesthesia ; Monitoring ; Selection of channels ; Automatic staging
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Abstract The conventional multichannel electroencephalogram is quite inconvenient for long-term monitoring in the operating theatre or intensive care unit. Recording of the EEG would be easier if a small number of channels was sufficient. Aiming at reduction of channels, leads from different regions of the scalp were analysed visually and with regard to their spectral content. Methods. Electrode placements corresponded to the International 10/20 System (Fig. 1). EEG recordings were made with a conventional device (ES 12000), a personal computer, and a spectral analyser. Two-channel recordings. Retrospective analysis was performed on data from 392 patients (age 14–90 years) whose anaesthesia was induced with various anaesthetics/narcotics, for instance thiopental, ketamine, etomidate, halothane, and enflurane. The EEG was recorded using C3–P3 and Cz–A1. For each patient the changes of spectral parameters during the course of the induction were plotted and visually analysed. For statistical analyses a 30-s epoch of each patient was randomly selected from the first few minutes after the beginning of induction. Ten-channel recordings. In ten gynaecological patients (age 26–55 years) EEG recordings were performed during induction of anaesthesia with thiopental in combination with fentanyl, N2O and O2. The set of channels consisted of Fz′–Cb1, F3′–Cb1, Cz–Cb1, C3–Cb1, P3–Cb1, Oz–Cb1, Fz′–F3′, F3′–C3, C3–P3, and P3–Oz. The electrodes Fz′ and F3′ were positioned on the forehead near to Fz and F3, respectively. These sites were chosen because they allow easy application of electrodes. The relationship between channels was calculated with Bravais-Pearson's coefficient of correlation for the power and the absolute power in the frequency bands delta (0.5–3.5 Hz), theta (3.5–7.5 Hz), alpha (7.5–12.5 Hz), and beta (〉12.5 Hz). Results. In visual and statistical analyses of the two- and ten-channel recordings under the influence of anaesthetics/narcotics, similar changes of EEG activity could be observed in all channels. Although differences in the absolute power of the frequency bands were present, there was high conformity in the composition of the spectral content of the different channels. Classification of the EEG into stages of anaesthesia by means of a single channel led to consistent results for all channels. Alpha activity as leading feature of the awake state predominated occipitally. In channels including the region around the ears, contamination with EKG artifacts was observed. Conclusions. EEG patterns under the influence of different anaesthetics/narcotics are adequately represented by a reduced number of channels. For the choice of an appropriate set of channels the following aspects should be considered. Contamination with artifacts should be as low as possible, electrode sites should easily be accessible, and special features of the awake state should be identifiable. Experience with routinely conducted EEG recordings in the operating theatre and the intensive care unit showed that the channels C3–P3 or C4–P4 provide a sufficient basis for automatic staging of the depth of anaesthesia, which is implemented in the EEG monitor Narkograph.
    Notes: Zusammenfassung EEG-Registrierungen mit einer größeren Anzahl von Ableitungen sind technisch aufwendig. Als Routinemonitoringverfahren z.B. zur Beurteilung der Effekte von Anästhetika/Narkotika bei der Steuerung der Narkose sind sie daher wenig geeignet. Die EEG-Ableitung wäre einfacher, wenn sie mit wenigen Kanälen erfolgen könnte. Daher sollten in dieser Arbeit EEG-Ableitungen von unterschiedlichen Regionen des Kopfs mit dem Ziel einer Kanalreduktion visuell und im Hinblick auf den spektralen Gehalt verglichen werden. Dazu wurden 392 EEG-Kurven mit zwei Kanälen aus Narkoseeinleitungen mit unterschiedlichen Anästhetika/Narkotika und zehn EEG-Aufzeichnungen mit jeweils zehn Kanälen bei Narkoseeinleitungen mit Fentanyl und Thiopental untersucht. Die visuellen und statistischen Auswertungen zeigen, daß unter dem Einfluß von Anästhetika/Narkotika in unterschiedlichen Ableitungen gleichartige EEG-Bilder auftreten und anhand einer einzelnen dieser Ableitungen eine Stadienklassifikation möglich ist. Bei eigenen Routinemessungen haben sich die Ableitungen C 3 −P 3 oder C 4 −P 4 als Grundlage für ein EEG-Monitoring in der Anästhesie als geeignet erwiesen. Diese Ableitungen bieten neben leichter Zugänglichkeit der Ableiteelektroden gute Voraussetzungen für die Erkennung des Wach-EEG und sind im Vergleich mit anderen Ableitungen artefaktarm.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Journal of molecular medicine 54 (1976), S. 625-632 
    ISSN: 1432-1440
    Keywords: Renal osteodystrophy ; Parathyroid hormone ; Vitamin D ; 25-Hydroxycalciferol ; Bone biopsy ; Renale Osteopathie ; Parathormon ; Vitamin D ; 25-Hydroxycalciferol ; Knochenbiopsie
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Bei 32 Patienten mit renaler Osteopathie wurden die Serumspiegel des immunoreaktiven Parathormons (iPTH) und des 25-Hydroxycalciferol (25(OH)D) bestimmt und mit den Ergebnissen der Knochenbiopsie und anderen klinischen Parametern korreliert. Das iPTH entsprach den Ergebnissen der Knochenhistologie, zeigte jedoch keine Beziehung zum Serumcalcium und der alkalischen Phosphatase, während eine statistisch signifikante Korrelation mit dem Serumphosphat bestand. Die 25(OH)D-Spiegel verhielten sich unabhängig vom histologischen Nachweis einer Osteomalazie oder vermehrter Knochenresorption, es fand sich eine Beziehung zwischen dem Vitamin-D-Metaboliten und dem Serumcalcium. Da die iPTH- und die 25(OH)D-Spiegel eine signifikante inverse Korrelation zeigten, wurde ein inhibitorischer Effekt des 25(OH)D auf die Nebenschilddrüsenfunktion bei Niereninsuffizienz diskutiert.
    Notes: Summary Immunoreactive parathyroid hormone (iPTH) and 25-hydroxycalciferol (25(OH)D) serum levels were determined in 32 patients with renal osteopathy, they were correlated with the results of bone biopsy and other clinical parameters. iPTH was closely related to bone histology, it did not correspond to serum calcium and alkaline phosphatase, but the correlation to serum phosphate was statistically significant. 25(OH)D levels were not related to the histological findings of osteomalacia or increased bone resorption, while a correlation between the vitamin D metabolite and serum calcium could be observed. Since iPTH and 25(OH)D levels exhibited a significant correlation, an inhibitory effect of 25(OH)D on parathyroid gland function in renal failure was discussed.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Journal of molecular medicine 65 (1987), S. 1095-1100 
    ISSN: 1432-1440
    Keywords: Osteoporosis ; Osteocalcin ; Bone
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The decoupling of bone formation and bone resorption causes an insidious bone loss that is responsible for the negative skeletal balance in the frequent form of low turnover osteoporosis. The reduction of bone formation can hardly be verified by clinical methods. Osteocalcin, a non-collagenous bone protein, has proved to be a useful new indicator of bone formation. To establish its predictable value, plasma levels of osteocalcin were compared to conventional serological data of bone turnover and to histomorphometric parameters of iliac crest trabecular bone. In cases of osteoporosis with normal bone turnover activity (as confirmed by histomorphometry) no differences were observed in any of our laboratory data including osteocalcin. However, there was a significant lower mean serum level of osteocalcin in a group of patients with histomorphometrically proven low turnover osteoporosis in comparison to those with normal bone turnover. Serum levels of osteocalcin below 2.0 ng/ml seem to indicate a low turnover in the individual case of osteoporosis while this is unlikely when serum levels above 6.0 ng/ml are measured (according to our RIA).
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1432-1440
    Keywords: Key Words Pancreas ; Islets of Langerhans ; Islet isolation ; Pancreas morphology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract In clinical islet transplantation to patients with type 1 diabetes mellitus, the number of isolated and purified islet has been identified as a key determinant for functional success of the islet graft [1]. With improved isolation methods based on the original procedure published by Ricordi et al. [2] yield and function of isolated islets were considerably enhanced. However, there is still a large variance in the number, purity, viability and secretory capacity of islets isolated from brain-dead human donor pancreata, significantly hampering utilization of human islet preparations derived from a single donor for one diabetic recipient. The reasons for the limited success in islet isolation and purification have not been clarified in detail yet. Recent studies have indicated, that donor preconditions, and a number of technical factors during organ procurement and the islet isolation process itself are critical to successful islet isolation [3, 4]. This study aimed at identifying distinct morphological and histopathological characteristics of the donor pancreas as determinants for the outcome of human islet isolation and purification.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Human genetics 〈Berlin〉 89 (1992), S. 122-122 
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A rare polymorphism of the probe p20 in the Duchenne muscular dystrophy gene is reported.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1432-0649
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Using a confocal microscope with a single-photon avalanche photodiode as detector, we studied photon bursts of single Rhodamine 6G (R6G) and Rhodamin B-zwitterion (RB) molecules in aqueous solution by excitation of the lowest excited singlet stateS 1 with a frequency-doubled titanium: sapphire laser. Multichannel scaler traces, the fluorescence autocorrelation function and fluorescence decay times determined by time-correlated single-photon counting have been measured simultaneously. The time-resolved fluorescence signals were analyzed with a maximum likelihood estimator. Fluorescence lifetime patterns in steps of 100 ps were generated by convolution with the excitation pulse. The lifetime of theS 1 state was derived from the Kullback-Leibler minimum discrimination information. We are able to demonstrate for the first time identification of two different single dye molecules via their characteristic fluorescence lifetimes of 1.79 ± 0.33 ns (RB) and 3.79 ± 0.38 ns (R6G) in aqueous solution.
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1432-072X
    Keywords: Aerobacter aerogenes ; Glyphosate ; 5-Enolpyruvyl-shikimic acid-3-phosphate synthase ; Resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The broadspectrum herbicide glyphosate (N-[phosphonomethyl]glycine), an inhibitor of the shikimate pathway enzyme 5-enolpyruvyl-shikimic acid-3-phosphate (EPSP)-synthase, inhibits the growth of Aerobacter aerogenes and causes the excretion of shikimic acid-3-phosphate. A strain of A. aerogenes, resistant to inhibition of growth by glyphosate, was isolated and found to have a glyphosate-insensitive EPSP-synthase and to no longer excrete shikimic acid-3-phosphate in the presence of glyphosate. Partial identity of EPSP-synthases from the glyphosate-sensitive and-resistant A. aerogenes strains was demonstrated by immunological procedures.
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 1432-1076
    Keywords: Key words Antithrombin III ; Coagulation ; Haemangiomatosis ; Kasabach-Merrit syndrome
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Diffuse infantile haemangiomatosis of the spleen is a very rare lesion. Large haemangiomas may cause trapping of platelets and coagulation disorders known as Kasabach-Merrit syndrome. We here report the case of an infant with splenic and pancreatic haemangiomatosis presenting with life-threatening thrombocytopenia, anaemia and intravascular coagulation. Diagnosis was hampered by reactive erythroblastosis and non-conclusive radiological findings. While treatment with corticosteroids was ineffective, administration of antithrombin III improved coagulation parameters. After splenectomy the child recovered promptly and has remained free of disease for 3 years to date. Conclusion Occult visceral haemangiomatosis without visible cutaneous haemangiomas should be included in the differential diagnosis of thrombocytopenia, anaemia and consumption coagulopathy. Antithrombin III treatment may be considered to overcome bleeding problems in patients with Kasabach-Merrit syndrome.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    European journal of clinical pharmacology 33 (1987), S. 487-491 
    ISSN: 1432-1041
    Keywords: theophylline ; furosemide ; drug interaction ; urinary flow ; renal clearance ; urinary excretion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Medicine
    Notes: Summary In new of previous contradictory results, the possible interaction between the loop diuretic furosemide and theophylline was re-evaluated in 12 healthy volunteers with a steady-state plasma theophylline level. Two doses of furosemide 20 mg at a 4 h interval did not influence the steady-state plasma concentration of theophylline despite causing a moderate diuresis. Urinary recovery of theophylline and its metabolites amounted to 106±21% of the dose without furosemide and 96±19% of the dose with furosemide, demonstrating that there was no influence on the enteral absorption of theophylline of the furosemide treatment. After the first dose of furosemide the fractional renal clearance (CLR1) of theophylline (fractional = hourly sampling period) changed in parallel with the urinary flow rate, without a significant difference between treatment with and without furosemide. After the second dose of furosemide, CLR1 was increased in the first hour and then it declined to levels far lower than the control value. This unexpected result could explain the unchanged plasma concentration of theophylline during furosemide treatment.
    Type of Medium: Electronic Resource
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