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  • 2020-2024
  • 2015-2019
  • 2005-2009
  • 1995-1999  (34)
  • 1975-1979  (132)
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  • 1
    Digitale Medien
    Digitale Medien
    Renewing olfactory receptor neurons in goldfish do not require contact with the olfactory bulb to develop normal chemical responsiveness (1997)
    Springer
    Journal of comparative physiology 181 (1997), S. 425-437 
    Details
    ISSN: 1432-1351
    Schlagwort(e): Key words Olfactory receptor cells ; Olfactory bulbectomy ; Olfactory axotomy ; Electrophysiology ; Electron microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Abstract This study investigated whether contact with the olfactory bulb was necessary for developing and renewing olfactory receptor neurons (ORNs) to attain normal odorant responsiveness, and whether the anatomical and functional recoveries of the olfactory epithelium were similar in both bulbectomized (BE) and bilaterally axotomized (AX) preparations. In vivo electrophysiological recordings were obtained in response to amino acids, a bile acid [taurolithocholic acid sulfate(TLCS)] and a pheromonal odorant [17α, 20β,-dihydroxy-4-pregnen-3-one (17,20P)] from sexually immature goldfish. Both transmission and scanning electron microscopy indicated that the olfactory epithelium degenerated in BE and AX goldfish. Within 1–2 weeks subsequent to the respective surgeries, responses to high concentrations (〉0.1 mmol · l−1) of the more stimulatory amino acids remained, whereas responses were no longer obtainable to TLCS and 17,20P. At 4 weeks, responses to amino acid stimuli recovered to control levels, while responses to TLCS and 17,20P were minimal. By 7 weeks post bilateral axotomy, the olfactory epithelium recovered to a condition similar to control sensory epithelium; however, the rate of degeneration and proliferation of receptor neurons in BE preparations appeared to remain in balance, thus blocking further recovery of the olfactory epithelium. At 7 weeks post surgery, odorant responses of AX and BE goldfish to TLCS and 17,20P were still recovering.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s003590050126
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  • 2
    Digitale Medien
    Digitale Medien
    Cytodifferentiation of atypical adenomatous hyperplasia and bronchioloalveolar lung carcinoma: immunohistochemical and ultrastructural studies (1997)
    Springer
    Virchows Archiv 431 (1997), S. 415-424 
    Details
    ISSN: 1432-2307
    Schlagwort(e): Key words Bronchioloalveolar lung carcinoma ; Atypical adenomatous hyperplasia ; Surfactant apoprotein ; Urine protein 1 ; Electron microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract  We used immunohistochemistry and electron microscopy to evaluate the differentiation of cells comprising atypical adenomatous hyperplasia (AAH; n = 26), early bronchioloalveolar lung carcinoma (BAC; n = 11), and overt BAC (n = 16), which are assumed to constitute a continuous spectrum of developmental steps of BAC. Surfactant apoprotein (SAP), a marker for type 2 alveolar cells, was expressed in cells from all the lesions of AAH, early BAC, and overt BAC. However, the proportion of SAP-positive cells decreased and their distribution became more heterogeneous with advancing lesion grade. Urine protein 1, which is identical to the Clara cell-specific 10 kDa protein, was expressed in 70% of overt BAC, whereas only 20% of early BAC showed weak reactivity and none of AAH lesions showed any reactivity at all. Ultrastructurally, type 2 alveolar cell differentiation was predominant among cells from AAH and early BAC. Our results suggest that precursor cells of BAC differentiate predominantly towards type 2 alveolar cells. Cells comprising overt BAC retain this differentiation phenotype, but to a reduced extent. In contrast, concomitantly with progression, cells with Clara cell differentiation emerge and their proportion increases. Such phenotypic changes may reflect metaplasia occurring in tumour cells during the development of BAC.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s004280050118
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  • 3
    Digitale Medien
    Digitale Medien
    Adenovirus-induced liver necrosis in a case of AIDS (1997)
    Springer
    Virchows Archiv 431 (1997), S. 469-472 
    Details
    ISSN: 1432-2307
    Schlagwort(e): Key words Adenovirus ; Liver pathology ; Electron microscopy ; AIDS
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract  Adenovirus-induced liver necrosis is rare. Before the era of AIDS (acquired immunodeficiency syndrome) this entity was seen predominantly in infants suffering from inborn immunodeficiency syndromes or from iatrogenic immunosuppression because of bone marrow or liver transplantation. Here, we report a case of a 30-year-old woman with AIDS who developed fever and rapidly progressing liver failure. A frozen section from a needle biopsy of the liver allowed a quick diagnosis of viral liver necrosis. The light-microscopic and electron microscopic aspects were typical of adenovirus infection and should be known to the surgical pathologist. The diagnosis was confirmed by immunohistochemistry and DNA hybridization analysis.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s004280050125
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  • 4
    Digitale Medien
    Digitale Medien
    Argyrophilic tau-positive twisted and non-twisted tubules in astrocytic processes in brains of Alzheimer-type dementia: an electron microscopical study (1997)
    Springer
    Acta neuropathologica 95 (1997), S. 28-39 
    Details
    ISSN: 1432-0533
    Schlagwort(e): Key words Astrocytes ; Alzheimer-type dementia ; Electron microscopy ; Glial fibrillary tangles ; Tau protein
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract This report concerns pathological astrocytic tubular structures (astrocytic tubules, As-Tbs) that coexist with glial filaments in astrocytic processes in brains with presenile-onset Alzheimer-type dementia. The formation of As-Tbs appears to be related to the duration of disease and the intensity of Alzheimer histopathology. In three cases in which the disease was of extremely long duration, As-Tbs were found in the frontal and temporal neocortices, the temporal pole and the hippocampus using electron microscopy, whereas they were not found in two cases with a long, but not extremely long, illness duration. As-Tbs were almost exclusively found in the highly devastated neuropil, and we could not find them in regions of moderate neuronal degeneration despite intensive inspection. As reported previously, some As-Tbs was seen adjacent to extracellular neurofibrillary tangles (NFTs) and in perivascular astrocytes. Our novel finding is that they can exist independently from these, in the highly devastated neuropil. Two types of As-Tbs were observed, twisted tubules with periodic constrictions at 50- to 80-nm intervals and non-twisted tubules where no constrictions were seen but which had a 15-nm fuzzy outer contour. They were positively stained by anti-human tau antibody, an antibody that does not recognize extracellular NFTs. Thus, it is most likely that As-Tbs are not the sequestration of extracellular NFTs, and that they are of astrocytic origin. Moreover, As-Tbs showed argyrophilia. As-Tbs appear indistinguishable from dystrophic neurites under the light microscope. The present data suggest that they may be more widely distributed in the damaged cerebral neuropil than previously thought.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s004010050762
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  • 5
    Digitale Medien
    Digitale Medien
    Scots pine needle injuries at subarctic industrial sites (1997)
    Springer
    Trees 11 (1997), S. 378-387 
    Details
    ISSN: 0931-1890
    Schlagwort(e): Key words Pinus sylvestris (L.) ; Electron microscopy ; Heavy metals ; Multi-stress-symptoms ; SO2
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract  Injuries to needles of Scots pines (Pinus sylvestris L.) growing in nutrient-poor soils on the Kola Peninsula collected in April 1991 were studied on a gradient of increasing distances (10 – 115 km) from the Monchegorsk nickel smelter, Russia, which emits SO2, Ni and Cu. The condition of the mesophyll cells was quantified from needles of the two latest age classes using a light and an electron microscope. The damage to the ultrastructure consisted of multistress symptoms caused by excess sulphur, heavy metals, frost, acidic precipitation and ozone. Injuries were most commonly manifested in the form of dark, irregularly shaped chloroplasts with protrusions and light thylakoids and plastoglobuli. These symptoms gradually disappeared with increasing distance and decreasing deposition rate. Concentrations of sulphur, copper and nickel decreased towards more distant sites where normal levels of the latter two elements were reached. Sulphur concentrations remained above background throughout the distance gradient. In the closest plots to the smelter area, cell collapse under the stomata and epidermis related to acute SO2 and heavy metal effects was found, whereas further away symptoms were more diverse, pointing towards the effects of ozone, acidic deposition and thereby decreased frost tolerance. The additive multistress symptoms were clearly seen in the area up to 40 km from the smelter where needle Cu concentration was above 110 ppm, Ni concentration above 39 ppm and S concentration above 1343 ppm.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s004680050099
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  • 6
    Digitale Medien
    Digitale Medien
    Phenotypic variability of CADASIL and novel morphologic findings (1997)
    Springer
    Acta neuropathologica 94 (1997), S. 247-254 
    Details
    ISSN: 1432-0533
    Schlagwort(e): Key words CADASIL ; Electron microscopy ; Granular osmiophilic material ; αB crystallin ; Heat ; shock proteins
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract Cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL) is a non-arterio-atherosclerotic, non-amyloidotic arteriopathy affecting preferentially the small arteries and arterioles of the brain. The morphologic hallmark is the presence of a characteristic granular alteration of the arterial media that ultrastructurally corresponds to the accumulation of electron-dense material surrounding the smooth muscle cells. Although the presence of this granular osmiophilic material (GOM) was originally described as limited to brain vessels, identical electron microscopic findings have been demonstrated in the media of peripheral tissue arteries, allowing for a pathologic diagnosis of the disease by a simple skin, muscle or nerve biopsy. We report some atypical features identified in our CADASIL patients that broaden the phenotypic expression of this disease. Firstly, we identified a cortical infarct in an otherwise typical CADASIL patient. Secondly, we observed GOM in skin arteries of a 30-year-old man with hemiplegic migraine, the son of a woman who had died with CADASIL. This confirms that it may be possible to diagnose the disease at a preclinical stage by the ultrastructural evaluation of peripheral tissue biopsy material, particularly for individuals for whom there is a supporting family history. Thirdly, ultrastructural examination of the skin, and subcutaneous and striated muscle of an unrelated and apparently sporadic patient with neuropathologic and neuroradiologic evidence of CADASIL in meningeal and cerebral vessels failed to reveal diagnostic lesions in peripheral arteries. Thus, the possibility of a false-negative pathologic diagnosis in patients with a clinicoradiologic diagnosis of CADASIL, if one relies solely on a peripheral tissue biopsy, does exist. Additionally, we have identified heat shock proteins (Hsp70 and αB crystallin) and ubiquitin in the vascular myocytes of affected arteries. αB crystallin also seemed to be deposited extracellularly, which suggests that GOM also might be immunoreactive for αB crystallin.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s004010050700
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  • 7
    Digitale Medien
    Digitale Medien
    Density and fine structure of peripheral nerves in various skin lesions of atopic dermatitis (1997)
    Springer
    Archives of dermatological research 289 (1997), S. 125-131 
    Details
    ISSN: 1432-069X
    Schlagwort(e): Key words Atopic dermatitis ; Peripheral nerve ;   ; Electron microscopy ; Protein gene product ; Substance P
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract The density and fine structure of the peripheral nerve system in various skin lesions of 64 patients with atopic dermatitis (AD) was quantitatively analyzed by immunohistochemical staining with antibodies directed against protein gene product (PGP) and substance P (SP). The density of PGP-positive peripheral nerves was 2.5 × 10 3 μm 2 /Δs (Δs = 0.24 mm 2 selected area) in early acute lesions, 3.8 × 10 3 μm 2 /Δs in subacute lesions, 4.9 × 10 3 μm 2 /Δs in lichenified lesions and 7.1 × 10 3 μm 2 /Δs in prurigo lesions of AD. The density of nerve fibers in subacute, lichenified and prurigo lesions was significantly higher than in uninvolved skin of AD patients (2.0 × 10 3 μm 2 /Δs). Electron microscopically, bulging of axons with many mitochondria and a loss of their surrounding sheath of Schwann cells suggests that the free nerve endings in skin lesions of AD are in an active state of excitation. Many pinocytotic vesicles in the periphery of basal keratinocytes facing nerve endings which contained many neurovesicles suggests reciprocal effects between keratinocytes and nerve endings. The number of SP-positive nerve fibers in AD lesions was far less than one-tenth of the number of PGP-positive nerve fibers.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s004030050167
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  • 8
    Digitale Medien
    Digitale Medien
    Effects of brefeldin-A and monensin on organelle distribution and morphology in the preimplantation mouse embryo (1997)
    Springer
    Development genes and evolution 206 (1997), S. 503-514 
    Details
    ISSN: 1432-041X
    Schlagwort(e): Key words Preimplantation mouse embryo ; Brefeldin-A ; Monensin ; Golgi ; Electron microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  The intracellular trafficking of integral membrane and secreted proteins is likely to be a key element involved in the morphogenesis and differentiation of the early mammalian embryo. In this study, we used transmission electron microscopy (TEM) to analyse the effects of brefeldin-A (BFA) and monensin, well known inhibitors of vesicular protein trafficking in somatic cells, on the structure of preimplantation mouse embryos. Both BFA and monensin distinctively altered the morphology of Golgi compartments in the blastomeres of treated morulae. BFA-treated morulae lacked recognizable Golgi complexes but possessed heterogeneous organelle clusters consisting of an abundance of smooth tubular and vesicular membrane compartments in addition to mitochondria, endosomes and lysosomes. Treatment of morulae with monensin was associated with swelling of Golgi compartments in addition to altering the morphology of mitochondria, lysosomes and the plasma membrane. BFA, and to a lesser extent monensin, inhibited cytokinesis as evidenced by the detection of binucleate blastomeres. In addition, BFA induced morulae to decompact. These latter effects have not been reported previously for these agents in mammalian somatic cell lines or other vertebrate or invertebrate embryos. These results provide the first demonstration of the structural effects of BFA and monensin on cells of the early mammalian embryo, some of which are consistent with the known actions of these agents on components of the vesicular protein trafficking system in mammalian somatic cells. This information serves as a foundation for the further use of these agents in studies of vesicular protein trafficking as an agent of preimplantation morphogenesis.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s004270050081
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  • 9
    Digitale Medien
    Digitale Medien
    Komibinierte neuronale und endokrine Tumoren der Sellaregion (1997)
    Springer
    Der Pathologe 18 (1997), S. 419-424 
    Details
    ISSN: 1432-1963
    Schlagwort(e): Schlüsselwörter Gangliozytom ; Gangliogliom ; Hypophysenadenom ; Immunhistologie ; Ultrastruktur ; Key words Gangliocytoma ; Ganglioglioma ; Pituitary adenoma ; Immunohistochemistry ; Electron microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Beschreibung / Inhaltsverzeichnis: Summary Gangliocytomas or gangliogliomas of the sellar region are very rare tumors. In a great proportion of those cases an adenoma of the anterior pituitary develops from the cell type that is hyperstimulated by the releasing hormone produced from the gangliocytoma. Five GHRH secreting gangliocytomas are reported. Four of these were localized adjacent to a GH secreting adenoma. In one case, no adenoma tissue was found beside the ganglicytoma. As only the adenomas can secrete GH, the adenomas and not the gangliocytomas are directly responsible for acromegaly so that such an adenoma has to be present in cases of acromegaly. A CRH secreting gangliocytoma was combined with an ACTH cell adenoma that had induced Cushing’s disease. A ganglioglioma of the posterior pituitary had led to an inappropriate secretion of Vasopressin. The morphology of the different tumors is presented.
    Notizen: Zusammenfassung Intraselläre Gangliozytome/Gangliogliome sind sehr seltene Tumoren, die in einem Großteil der Fälle ein Hypophysenadenom aus dem Zelltyp heraus entstehen lassen, der durch das gebildete Releasinghormon überstimuliert wird. Es wird über 5 GHRH-bildende Gangliozytome berichtet, von denen 4 einem STH-bildenden Hypophysenadenom benachbart waren. Ein Fall enthielt keine Adenomanteile. Diese müssen in Anbetracht der klinisch vorhandenen Akromegalie aber vorhanden sein, da nur das jeweilige Adenom, nicht aber die Gangliozytome für die Überfunktion unmittelbar verantwortlich sind. Ein CRH-bildendes Gangliozytom war mit einem zum Morbus Cushing führenden ACTH-Zelladenom kombiniert. Ein Gangliogliom des Hinterlappens hatte zu einer inadäquaten Vasopressinsekretion Anlaß gegeben. Die Morphologie der Tumoren wird dargelegt.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s002920050236
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  • 10
    Digitale Medien
    Digitale Medien
    Ultrastructural subtypes of glutamate-immunoreactive terminals on rat trigeminal motoneurones and their relationships with GABA-immunoreactive terminals (1997)
    Springer
    Experimental brain research 114 (1997), S. 99-116 
    Details
    ISSN: 1432-1106
    Schlagwort(e): Key words Synapse ; Axo-axonic synaptic contacts ; Trigeminal motor nucleus ; Immunogold ; Electron microscopy ; Rat
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract  Electron-microscopic immunolabelling methods were used to study the relationships between glutamate-immunoreactive and γ-aminobutyric acid (GABA)-immunoreactive synapses on trigeminal motoneurones labelled by the retrograde transport of horseradish peroxidase. Serial sections were cut through the motor nucleus, alternate sections were incubated with antibodies to glutamate and GABA, and the immunopositive nerve terminal profiles were recognized using a quantitative, postembedding immunogold method. Boutons exhibiting high levels of glutamate immunoreactivity and GABA-immunoreactive boutons both formed axo-dendritic and axo-somatic synaptic contacts on labelled motoneurones. Boutons strongly immunopositive for glutamate were not immunopositive for GABA, and vice versa. Strongly glutamate immunoreactive boutons received axo-axonic synaptic contacts but did not form such contacts, while GABA-immunoreactive boutons formed axo-axonic synapses but did not receive them. The presynaptic elements at all axo-axonic synapses on to glutamate-immunoreactive boutons sampled were GABA-immunopositive. These data provide ultrastructural evidence in support of the roles of glutamate and GABA as transmitters at synapses on trigeminal motoneurones, and for presynaptic control of transmission at glutamatergic synapses by GABA acting at receptors at axo-axonic synapses. The vast majority (more than 90%) of strongly glutamate immunoreactive boutons contained spherical synaptic vesicles, in contrast to GABA-immunoreactive boutons, which contained pleomorphic vesicles. Most of the glutamate-immunoreactive boutons (67%) formed asymmetrical synaptic active zones, many of which (47% of total) were associated with subsynaptic dense ”Taxi” bodies (T-terminals), while a smaller population of boutons (21%) formed symmetrical synapses, and a few (11%) made synapses associated with subsynaptic cisternae (C-terminals). The heterogeneity of active zone ultrastructure of boutons identified as being glutamatergic on the basis of their high levels of immunolabelling is discussed in relation to possible differences in co-transmitters released, origins of the synaptic input or post-synaptic receptor subtypes activated.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/PL00005627
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  • 11
    Digitale Medien
    Digitale Medien
    Type III Ehlers-Danlos syndrome and pregnancy (1997)
    Springer
    Archives of gynecology and obstetrics 261 (1997), S. 39-43 
    Details
    ISSN: 1432-0711
    Schlagwort(e): Key words: Ehlers-Danlos type III ; Pregnancy ; Electron microscopy ; Prenatal diagnosis ; Premature rupture of membranes
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract. We report a case of type III Ehlers-Danlos syndrome with a favourable outcome. We review the literature and do not consider that pregnancy in patients with type III Ehlers-Danlos Syndrome represents a high risk situation.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s004040050196
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  • 12
    Digitale Medien
    Digitale Medien
    Light and electron microscopic investigation of the process of healing of the naevus of Ota by Q-switched alexandrite laser irradiation (1997)
    Springer
    Virchows Archiv 431 (1997), S. 63-71 
    Details
    ISSN: 1432-2307
    Schlagwort(e): Key words Naevus of Ota ; Q-switched alexandrite laser ; Light microscopy ; Electron microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract  Melanocytes in the naevus of Ota were destroyed by irradiation using the Q-switched alexandrite laser. This laser is highly selective and highly absorbed by melanosomes. Other cells and tissue components of the dermis remained almost intact. Melanosomes were vaporized or fragmented to subelectron microscopical size, or degenerated. If the irradiated energy was sufficient, melanocytes vanished and large vacuoles several times the size of dermal melanocytes formed at the sites. If it was too weak, dermal melanocytes were also vaporized, but vacuoles formed within them. Nuclei were no longer discernible. Following irradiation macrophages infiltrated the irradiated areas and scavenged degenerated melanosomes and cellular debris. Thus, discoloration of the skin was markedly reduced. Although a few melanocytes and melanophages remained, pigmentation cleared to a satisfactory level. Melanocytes and keratinocytes were also injured in the epidermis; however, the epidermis recovered completely. No scarring was observed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s004280050070
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  • 13
    Digitale Medien
    Digitale Medien
    Neuropeptide Y-immunoreactive intracardiac neurones, granule-containing cells and nerves associated with ganglia and blood vessels in rat and guinea-pig heart (1997)
    Springer
    Cell & tissue research 289 (1997), S. 445-454 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Key words: Intracardiac neurones ; Innervation ; Heart ; Neuropeptide Y ; Immunocytochemistry ; Electron microscopy ; Rat (Sprague Dawley) ; Guinea-pig (Dunkin Hartley)
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Abstract. Intrinsic neuropeptide Y-containing neurones in rat and guinea-pig hearts were studied at the ultrastructural level by the pre-embedding peroxidase-antiperoxidase immunocytochemical technique. Intracardiac neuronal cell bodies were often weakly or moderately immunostained, and the labelling was usually pronounced in the Golgi complex, multivesicular bodies, some cisterns of granular endoplasmic reticulum and large granular vesicles. Neuropeptide Y-immunoreactive nerve fibres were also observed in association with intracardiac neurones. A subpopulation of neuropeptide Y-immunoreactive granule-containing cells in the rat heart are described for the first time and were very heavily labelled; other granule-containing cells were non-immunoreactive, but were contacted by neuropeptide Y-containing nerves. Preterminal regions of nerve fibres that were located in nerve bundles were only weakly neuropeptide Y-immunoreactive, in contrast to the heavy labelling observed in varicosities that contained many synaptic vesicles. Many neuropeptide Y-immunoreactive nerve fibres were associated with the coronary vasculature and were particularly prominent in the walls of small arteries and arterioles where labelled nerve varicosities were present close to the smooth muscle cells. Immunoreactive nerves were also seen in the myocardium, usually near to capillaries. In axonal varicosities, the central core of large granular vesicles was immunolabelled, and electron-dense immunoreactive material outlined the membranes of small and large clear vesicles. The significance of neuropeptide Y-immunoreactive intracardiac neurones and granule-containing cells and the origin of associated labelled nerve fibres in the heart are discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s004410050890
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  • 14
    Digitale Medien
    Digitale Medien
    Immuno-electron microscopy reveals that the excitotoxin quinolinate is associated with the plasma membrane in human peripheral blood monocytes/macrophages (1997)
    Springer
    Cell & tissue research 290 (1997), S. 633-639 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Key words: Quinolinic acid ; Interferon-γ ; Kynurenine ; Electron microscopy ; Immunocytochemistry ; Excitotoxicity ; Human
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Abstract.  Quinolinate (QUIN), a tryptophan-derived excitotoxin, was localized ultrastructurally in human peripheral blood monocytes/macrophages (MØ) by immuno-electron microscopy. A combined carbodiimide/glutaraldehyde/paraformaldehyde-based fixation procedure was developed for optimal retention of QUIN in the cell as well as minimal loss of ultrastructure; a silver-enhanced colloidal gold detection system was used for electron-microscopic analysis. Gold particles representing QUIN immunoreactivity were associated with the inner side of the plasma membrane in normal MØ. The number of gold particles increased significantly when QUIN levels were elevated by treatment with its precursor kynurenine, but location of the gold particles remained essentially the same under this condition. Treatment with interferon-γ increased the number of Golgi bodies, vacuoles and pseudopodia, reflecting the activated state of the cell. Significantly increased numbers of gold particles representing QUIN were detectable in approximately the same location as in the case of kynurenine treatment. Combined treatment with kynurenine and interferon-γ maximally increased the number of gold particles at the periphery of the cell. The pseudopodia were intensely stained with gold particles, while they were not detectable in the inner part of the cytoplasm or in any other organelle even under this activated condition. The significance of the specific location of QUIN revealed in the present study and its relation to the release and subsequent actions of QUIN are discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s004410050969
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  • 15
    Digitale Medien
    Digitale Medien
    Synaptic exocytosis of dense-core vesicles in blue crab (Callinectes sapidus) stomach muscles (1997)
    Springer
    Cell & tissue research 289 (1997), S. 517-526 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Key words: Neuromuscular junction ; Synaptic vesicles ; Vesicle fusion ; Electron microscopy ; Callinectes sapidus (Crustacea)
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Abstract. Neuromuscular terminals of a single motoneuron to four muscles (CPV7a, GM5a, CV2, and CV3) in the stomach of the blue crab Callinectes sapidus showed structural evidence for the exocytotic release of dense-core vesicles exclusively at synapses. The primary evidence was the appearance of dense cores in the synaptic cleft, accompanied by indentations of the presynaptic or postsynaptic membrane. In their simplest form, these consisted of an omega-shaped figure of the presynaptic membrane enclosing one dense core, denoting release of a single dense-core vesicle. A larger indentation of the presynaptic membrane enclosing several dense cores denoted multiple release. A more complex form of multiple release was where the presynaptic membrane was normal, but the postsynaptic membrane elaborated into a sac projecting into the granular sarcoplasm and filled with dense cores. The postsynaptic sac in some instances was compressed into a thin, fingerlike extension, which lacked dense cores and, at its distal end, separated into small cisternae, suggesting a mechanism for membrane recycling. Profiles depicting single and multiple releases of dense-core vesicles were found more frequently at neuromuscular terminals that release relatively large amounts of transmitter with a single stimulus, such as CV2 and CV3, compared to those releasing smaller amounts, such as CPV7a and GM5a. The disparity in release sites among the four muscles of this single motor unit and the fact that many of the multiple-release figures were closely adjacent to the active zones for transmitter release suggest a possible modulatory role for dense-core vesicles in synaptic transmission. Such modulation may be long lasting, as implied by the postsynaptic sacs, which may permit prolonged release of the contents of their dense cores into the synaptic cleft. This is in keeping with the functional role of these stomach muscles, which is to be continuously active for long periods of time.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s004410050897
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  • 16
    Digitale Medien
    Digitale Medien
    Immunoreactive excitatory amino acids in the parietal eye of lizards, a comparison with the pineal organ and retina (1997)
    Springer
    Cell & tissue research 287 (1997), S. 275-283 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Key words: Parietal eye ; Pineal organ ; Retina ; Glutamate ; Aspartate ; Immunocytochemistry ; Electron microscopy ; Lacerta muralis ; Lacerta agilis ; Lacerta viridis (Lacertilia)
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Abstract. The fine structure of the organ and the localization of the excitatory amino acids glutamate and aspartate were studied in the parietal eye of lizards by postembedding immunoelectron microscopy. The parietal eye contains cone photoreceptor cells, secondary neurons, and ependymal and lens cells. The photoreceptors form long inner and outer segments, some of them being paired as ”twin-photoreceptors” by zonulae adherentes. Perikarya of neurons bear sensory cilia (containing 9×2+0 pairs of tubules) extending into the intercellular space. No neurohormonal terminals are present in the parietal eye. A higher immunoreactivity to glutamate than to aspartate is found in the photoreceptors and in the secondary neurons of the parietal eye. Glutamate immunogold labeling is more intense in the axonal processes of photoreceptors and neurons and in most of the nerve fibers of the parietal nerve running to the brain stem. Weak aspartate and glutamate immunoreactivity can be detected in the ependymal and lens cells. A similar distribution of immunoreactive amino acids is found in the photoreceptors, secondary neurons, and ependymal glial elements of the pineal organ, and retina of the lateral eye of the same animals. Immunoreactive glutamate accumulates in the axons of photoreceptors and secondary neurons of the parietal eye suggesting that this excitatory amino acid acts as a synaptic mediator in the neural efferentation of the organ. Thus, the efferent light-conducting pathway of the parietal organ is similar to that of the pineal organ and lateral eye retina. As the Mullerian cells of the retina, the ependymal and lens cells of the parietal eye and the ependymal-glial cells of the pineal organ may play a role in the metabolism and/or elimination of excitatory amino acids released by photoreceptors.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s004410050752
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  • 17
    Digitale Medien
    Digitale Medien
    Expression of basic fibroblast growth factor-like immunoreactivity in the nuclei of regenerating hepatocytes (1997)
    Springer
    Cell & tissue research 288 (1997), S. 517-527 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Key words: Liver ; Subcellular fractions ; Immunohistochemistry ; Electron microscopy ; Rat (Wistar)
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Abstract. This study, utilizing rats subjected to two-thirds partial hepatectomy or sham operation, was designed (1) to investigate the content of basic fibroblast growth factor (bFGF) in the subcellular fractions of regenerating and sham-operated rat livers by immunoblot experiments and enzyme-linked immunosorbent assay (ELISA), (2) to show that bFGF immunoreactivity and proliferating cell nuclear antigen (PCNA) immunoreactivity are markers for hepatocellular mitosis before and after partial hepatectomy, and (3) to observe the location and fine structure of the bFGF immunoreaction within the regenerating liver with special attention to bFGF immunoreactivity in the nuclei of regenerating hepatocytes. Immunoblot experiments and ELISA showed a transient increase in high-molecular-weight forms of bFGF in the nuclear subcellular fraction of regenerating liver 48 h after partial hepatectomy. By light microscopy, bFGF and PCNA immunoreactivities were detected in the nuclei of regenerating hepatocytes. Electron microscopy demonstrated bFGF-like immunoreactivity mainly in the nuclear euchromatin and rarely in the heterochromatin or nucleoli of regenerating hepatocytes. The transient increase in high-molecular-weight forms of bFGF in the nuclear euchromatin of regenerating hepatocytes, together with the concomitant expression of PCNA in the regenerating liver, suggests an important role of the high-molecular-weight forms of bFGF in hepatocyte proliferation and/or mitosis, although authentic bFGF with a molecular form of 18 kDa is not considered to be involved in hepatic regeneration.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s004410050837
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  • 18
    Digitale Medien
    Digitale Medien
    Osmoregulatory mutants that affect the function of the contractile vacuole inChlamydomonas reinhardtii (1997)
    Springer
    Protoplasma 200 (1997), S. 99-111 
    Details
    ISSN: 1615-6102
    Schlagwort(e): Chlamydomonas reinhardtii ; Contractile vacuole ; Electron microscopy ; Videomicroscopy ; Osmoregulation ; Osmoregulatory mutants
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Four independent osmoregulatory mutants,osml, osm3,osm4, and osm7, were isolated on the basis of their requirement for growth medium of high osmotic strength. In normal low-osmoticstrength medium, in contrast to wild-type cells, the mutants grow poorly or not at all; in distilled water mutant cells are immobilized and eventually swell and burst. The mutants were examined by ordinary brightfield and phase-contrast microscopy, videomicroscopy, and electron microscopy. The four mutants showed different defects in the contractile vacuole (CV) cycle. Timing of various stages of the CV cycle showed thatosm1 was affected primarily in the early stage of the cycle when the CV begins to grow,osm3 primarily in midcycle when vacuoles fuse to form the CV proper,osm7 at a late stage of the cycle at docking and fusion of the CV with the plasma membrane, andosm4 during contraction of the CV. At the electron microscopic level, in dilute medium, mutant cells by comparison with wild-type cells had large autophagosomes, swollen mitochondria, and dilated ER cisternae. Although electron microscopy showed general abnormalities of the contractile vacuoles consistent with the videomicroscopic observations of living cells, no obvious vacuole membrane abnormalities were seen which would explain the mutational defects. The mutations help define the separate processes that contribute to the coordinated CV cycle inChlamydomonas, and open the way to eventual isolation of some of the genes responsible for CV function.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01280738
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  • 19
    Digitale Medien
    Digitale Medien
    Structure and behavior of contractile vacuoles inChlamydomonas reinhardtii (1997)
    Springer
    Protoplasma 198 (1997), S. 73-84 
    Details
    ISSN: 1615-6102
    Schlagwort(e): Chlamydomonas reinhardtii ; Contractile vacuole ; Osmoregulation ; Videomicroscopy ; Electron microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The contractile vacuole (CV) cycle ofChlamydomonas reinhardtii has been investigated by videomicroscopy and electron microscopy. Correlation of the two kinds of observation indicates that the total cycle (15 s under the hypo-osmotic conditions used for videomicroscopy) can be divided into early, middle, and late stages. In the early stage (early diastole, about 3 s long) numerous small vesicles about 70–120 nm in diameter are present. In the middle stage (mid-diastole, about 6 s long), the vesicles appear to fuse with one another to form the contractile vacuole proper. In the late stage (late diastole, also about 6 s long), the CV increases in diameter by the continued fusion of small vesicles with the vacuole, and makes contact with the plasma membrane. The CV then rapidly decreases in size (systole, about 0.2 s). In isosmotic media, CVs do not appear to be functioning; under these conditions, the CV regions contain numerous small vesicles typical of the earliest stage of diastole. Fine structure observations have provided no evidence for a two-component CV system such as has been observed in some other cell types. Electron microscopy of cryofixed and freeze-substituted cells suggests that the irregularity of the profiles of larger vesicles and vacuoles and some other morphological details seen in conventionally fixed cells may be shrinkage artefacts. This study thus defines some of the membrane events in the normal contractile vacuole cycle ofChlamydomonas, and provides a morphological and temporal basis for the study of membrane fusion and fluid transport across membranes in a cell favorable for genetic analysis.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01282133
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  • 20
    Digitale Medien
    Digitale Medien
    Selective UV-induced damage to short-wavelength receptor cells in the butterflyPapilio xuthus (1997)
    Springer
    Protoplasma 200 (1997), S. 112-115 
    Details
    ISSN: 1615-6102
    Schlagwort(e): Electron microscopy ; UV-induced cell damage ; Photoreceptor ; Eye ; Lepidoptera
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary A technique that allows the specific inactivation of short-wavelength-sensitive photoreceptive cells has been needed for a long time. Such a technique could be useful in studies on the role(s) of UV-receptors in circadian rhythmicity, recognition of floral patterns, homing behaviour, and mate selection in arthropods. We provide ultrastructural evidence that short-wavelength receptor cells can be selectively damaged without affecting other spectral-cell types. Since the method does not require the killing of the experimental animal, the latter can be used in behavioural or other follow-up tests.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01280739
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  • 21
    Digitale Medien
    Digitale Medien
    Electron microscopic and immunomorphological investigations on the mucosa of the human paranasal sinuses (1997)
    Springer
    European archives of oto-rhino-laryngology and head & neck 254 (1997), S. 230-235 
    Details
    ISSN: 1434-4726
    Schlagwort(e): Paranasal sinus mucosa ; Collagen type VII ; Subepithelial connective tissue ; Electron microscopy ; Immunofluorescence microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract The morphology of the mucosa from the human paranasal sinuses was investigated by electron microscopy. A total of 27 specimens was taken from 11 patients following midfacial fractures. All tissue samples were biopsied during surgery after informed consent had been given. In accordance with light microscopic investigations, the mucosa represented a highly prismatic epithelium consisting of kinocilia-carrying and mucus-producing (goblet) cells. Other cell types, such as those occurring in the respiratory epithelium of other areas, could not be demonstrated. Electron microscopic and immunomorphological investigations revealed collagen type VII beneath the lamina densa of the basal lamina. According to findings obtained to date, this collagen type accompanies only a multilayered epithelium. Another peculiarity was the small number of basophils and eosinophils. Pronounced acute reactions of the mucosa in this area cannot be expected, which is in contrast to that of the nasal mucosa.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00874094
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  • 22
    Digitale Medien
    Digitale Medien
    Ultrastructural findings of the macula utriculi in a case of a petrous apex cholesteatoma: a comparison with findings in a patient with an acoustic neuroma (1997)
    Springer
    European archives of oto-rhino-laryngology and head & neck 254 (1997), S. 255-258 
    Details
    ISSN: 1434-4726
    Schlagwort(e): Electron microscopy ; Inner ear ; Utricle ; Cholesteatoma ; Acoustic neuroma
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract The morphological characteristics of the vestibular sensory cells of the macula utriculi obtained during surgery in a patient with a petrous apex cholesteatoma were examined using scanning and transmission electron microscopy. Findings were compared to cells studied in a patient with acoustic neuroma. Scanning electron microscopy showed that compared to the apparently normal cells in the acoustic neuroma case, most sensory cells in the cholesteatoma case had large cuticular plates, irregular locations of cilia and no clear polarizations. Supporting cells showed profuse short microvilli on the whole surface. With transmission electron photomicrographs, type I hair cells were not seen and certain morphological changes were observed in type-1I-like cells and supporting cells. We presume that the degenerative changes in the vestibular epithelia were due to circulatory disturbances and/or direct pressure applied to the vestibular nerve at the internal auditory canal, with subsequent involvement of the macula utriculi.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00874100
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  • 23
    Digitale Medien
    Digitale Medien
    Electron microscopic localization of nitric oxide I synthase in the organ of Corti of the guinea pig (1997)
    Springer
    European archives of oto-rhino-laryngology and head & neck 254 (1997), S. 396-400 
    Details
    ISSN: 1434-4726
    Schlagwort(e): Guinea pig hair cells ; Electron microscopy ; Immunocytochemistry ; Nitric oxide synthase ; Sound transduction
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract Nitric oxide synthase (NOS) activity has been detected previously in the mammalian cochlea at a light microscopic level. Here we present results of electron microscopic analysis for post-embedding immunoreactivity of neural-type NOS I in the cochlea of the guinea pig. Strong enzyme immunoreactivity was identified in the cytoplasm of inner and outer hair cells. Gold-labeled NOS I antibodies were mainly located in electron-dense areas of the cytoplasm, whereas electron-lucent regions of the receptor cells were nearly free from any immunoreactivity. In both types of hair cells anti-NOS I antibodies were also visible in the cuticular plates, hair bundles and nuclei. Further ultrastructural analysis revealed that the submembranous cisternae of the outer hair cells were nearly free from any reaction product, demonstrating that the whole cytoplasm of this hair cell was not immunoreactive. Other NOS I immunoreactivity was identified in the cuticular plates of the inner and outer pillar cells and in the cytoskeletal elements located in the apical parts of Deiter cells, forming the lamina reticularis or in cytoskeletal-containing regions in basal Deiter cells. Anti-NOS antibodies were visible in the nuclei of various cell types. Our findings suggest that nitric oxide produced by NO I synthase in the organ of Corti may act as a modulator of hair cell physiology during the processes of signal transduction with frequence selectivity.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01642558
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  • 24
    Digitale Medien
    Digitale Medien
    Immunohistochemical and electron microscopical characterization of stromal cells in nasopharyngeal angiofibromas (1997)
    Springer
    European archives of oto-rhino-laryngology and head & neck 254 (1997), S. 196-199 
    Details
    ISSN: 1434-4726
    Schlagwort(e): Nasopharyngeal angiofibroma ; Ultrastructure ; Myofibroblast ; Immunohistochemistry ; Electron microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract Twenty-eight cases of nasopharyngeal angiofibroma were studied immunohistochemically for cytoskeletal phenotyping of stromal cells. Electron microscopy was also used to study the ultrastructure of five of the tumors. All typical stromal cells showed intensive immunostaining for vimentin, but were negative for smooth muscle actin and desmin. Ultrastructurally, most of these cells appeared to be exclusively fibroblasts. However, in some areas stromal cells were seen that morphologically resembled myofibroblasts by their shapes and arrangement, and were characterized by the coexpression of vimentin and smooth muscle actin. Electron microscopy confirmed their myofibroblastic nature. The present study showed that the typical stromal cells in nasopharyngeal angiofibromas were fibroblasts and not myofibroblasts. In these tumors myofibroblasts occurred only focally, in connection with fibrotic areas and exclusively as a vimentin+/actin+ cytoskeletal phenotype. This indicates that myofibroblasts are not primary stromal tumor cells in nasopharyngeal angiofibromas, but occur due to regressive changes.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00879273
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  • 25
    Digitale Medien
    Digitale Medien
    Morphological and cytochemical changes in human mast cells during culture (1997)
    Springer
    Medical molecular morphology 30 (1997), S. 25-30 
    Details
    ISSN: 1860-1499
    Schlagwort(e): Mast cell ; Culture ; Electron microscopy ; Carbohydrate cytochemistry ; Peroxidase
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract Mast cells were cultured from human umbilical cord blood CD34-positive cells in the presence of interleukin (IL)-6 and stem cell factor (SCF). Electron microscopically, a particulate or beaded structure, the prominent structure of tryptase-positive mast cell (T mast cell) granules, was frequently observed in 54-day cultures. Straight or coiled lamellae, often observed in the granules of tryptase-and chymase-positive mast cells (TC mast cells), were observed in the granules in 75-day cultures. Cytochemically, the peroxidase activity of the granules was positive but decreased during the term of culture. Glycogen particles were abundant in 54-day cultures, and scarce in 75-day cultures. These findings suggest that the mast cells matured and changed in phenotype during the culture's term.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01458348
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  • 26
    Digitale Medien
    Digitale Medien
    An ultrastructural study of pemphigoid vegetans, characterizing the possible mechanism of vegetation (1997)
    Springer
    Medical molecular morphology 30 (1997), S. 48-54 
    Details
    ISSN: 1860-1499
    Schlagwort(e): Pemphigoid vegetans ; Lamina densa ; Mast cell ; Vegetation ; Electron microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract Ultrastructural changes in the skin lesions of pemphigoid vegetans were observed by transmission electron microscopy. Two significant changes were recognized at the vegetating site: (1) The basal lamina was sometimes interrupted and detached from the basal cells, protruding into the dermis to form folds. In the basal cells, hemidesmosomes were not decreased in number but tonofilament bundles were fewer than normal. Occasionally, basal cell processes extended beyond the basal lamina through its gap. These features resembled the epidermal growth factor-induced morphological changes of cultured skin. It was suspected that the abnormal attachment of the basal lamina to the basal cell was associated with keratinocyte proliferation. (2) Mast cells were encountered in the epidermis abutting on the basal lamina. It is uncommon to find mast cells in the epidermis. Mast cells in the epidermis were reported at the erythematous regions which were adjacent to the bullae of bullous pemphigoid, and were thought to have an important role in forming blisters. It was suggested that early changes in blister formation occur at the vegetating site.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01458352
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  • 27
    Digitale Medien
    Digitale Medien
    Morphological changes of c-Fos-like immunoreactivity in rat cerebral cortex after cerebral ischemia and reperfusion with special reference to vasoactive intestinal polypeptide (1997)
    Springer
    Medical molecular morphology 30 (1997), S. 111-118 
    Details
    ISSN: 1860-1499
    Schlagwort(e): c-Fos ; VIP ; Electron microscopy ; Immunocytochemistry ; Cerebral ischemia
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract We investigated morphological changes in neurons with c-Fos-like immunoreactivity (c-Fos-LI) after cerebral ischemia by light and electron microscopic immunocytochemistry. Strong c-Fos-LI was observed in layers II–VI of the cerebral cortex with an especially abundant distribution in the nuclei of layers II, IV, and VI ipsilateral to the lesioned side. Reperfusion after ischemia had a greater effect on the expression of c-Fos-LI than did permanent ischemia. Vasoactive intestinal peptide (VIP)-positive neurons were seen scattered in layers II–V of the cerebral cortex. Some VIP-positive neurons showed c-Fos-LI after ischemia. Electron microscopy revealed c-Fos-LI in euchromatin in the nuclei of c-Fos-positive cells. Dilatation of the cisternae of the rough endoplasmic reticulum and the presence of numerous secondary lysosomes were found in neurons on the lesioned side after 12h of reperfusion. Some VIP-containing neurons revealed c-Fos-LI with reperfusion after ischemia by a double immunostaining method on the same tissue section. These findings suggest that ischemia potentiates c-fos expression in VIP- or other transmitter- or modulator-containing neurons, thereby protecting from neuronal cell death.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01545311
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  • 28
    Digitale Medien
    Digitale Medien
    Morphological changes ofHelicobacter pylori after antibacterial therapy: an electron microscope study (1997)
    Springer
    Medical molecular morphology 30 (1997), S. 131-137 
    Details
    ISSN: 1860-1499
    Schlagwort(e): Helicobacter pylori ; Electron microscopy ; Three-dimensional reconstruction ; Eradication ; 13C-urease breath test ; Coccoid form
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract To investigate the effect of morphological change ofHelicobacter pylori on gastric mucosal inflammation,H. pylori was examined by means of an electron microscope and computer-assisted three-dimensional reconstruction before and after antibacterial therapy. The results showed that the bacterium was usually present in different forms and sizes. Most of these variations are helicobacter shaped, 3–5 μm long (43%), or campylobacter shaped, 1–3 μm long (38%); fewer are round or coccoid (19%), and only 0.5–1 μm long. Active inflammation and an abnormal amount of mucus in the epithelial cells are the main changes seen in gastric mucosa withH. pylori infection. In some cases, 6 weeks after antibacterial therapy,H. pylori could still be observed by toludine blue, with slight positive results withH. pylori-antibody-labeled immunostaining. In contrast to the bacteria before antibacterial therapy, in mostH. pylori-positive cases usually only a minute number of bacteria were seen in coccoid form. These surviving coccoid-form bacteria showed thickened walls, and the surrounding periplasmic space was reduced or nonexistent. The epithelial cells were almost normal and13C-text negative. Although neutrophil infiltration was seldom seen, there were still some surviving bulblike cells with excess amounts of mucus in the gastric pits. It is suggested that the coccoid form ofH. pylori may be a static bacterium without urease production. For therapeutic decisions, it may be more important to identify whether there is an active inflammation in the gastric mucosa than to detectH. pylori.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01545314
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  • 29
    Digitale Medien
    Digitale Medien
    Aneurysmal (angiomatoid) fibrous histiocytoma of the skin: an unusual variant of dermatofibroma (1997)
    Springer
    Medical molecular morphology 30 (1997), S. 163-169 
    Details
    ISSN: 1860-1499
    Schlagwort(e): Dermatofibroma ; Aneurysmal ; (angiomatoid) fibrous histiocytoma ; Immunohistochemistry ; Electron microscopy ; Skin
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract We present a rare case of aneurysmal (angiomatoid) fibrous histiocytoma (AAFH) of the skin on the back of a 40-year-old Japanese man. Histologically, the tumor was characterized by massive proliferation of fibroblastic and histiocytic cells, prominent aggregation of hemosiderin pigment, and the presence of blood-filled tissue spaces devoid of an endothelial lining within a capillaryrich stroma. Immunohistochemically the tumor cells were immunoreactive for vimentin and for Factor XIIIa or Mac387. Ultrastructural study revealed that the tumor was composed mainly of fibroblast-like cells intermingled with histiocyte-like cells and intermediate cells with combined features of the two types of cells. These findings support the fibrohistiocytic origin of aneurysmal (angiomatoid) fibrous histiocytoma. In addition, ultrastructural examination seems quite useful to differentiate from other cutaneous neoplasms with architectural and cytological similarities to this tumor.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01545318
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  • 30
    Digitale Medien
    Digitale Medien
    Osteoclast-like giant cell tumor of the pancreas: an immunohistochemical and ultrastructural study (1997)
    Springer
    Medical molecular morphology 30 (1997), S. 216-220 
    Details
    ISSN: 1860-1499
    Schlagwort(e): Osteoclast-like giant cell tumor ; Pancreas ; Immunohistochemistry ; Electron microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract We report a rare case of osteoclast-like giant cell tumor of the pancreas in a 70-year-old Japanese woman. The tumor was composed of a proliferation of ovoid to spindle-shaped mononuclear cells admixed with osteoclast-like giant cells. The tumor cells were immunore-active for vimentin, ±1-antitrypsin, and CD68. In ultrastructural examination, the giant cells resembled osteoclasts, and the mononuclear stromal cells had fibroblastic and histiocytic features. No elements of epithelial differentiation were found in this tumor. These findings suggest that this tumor had a derivation similar to giant cell tumor of bone.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01545773
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  • 31
    Digitale Medien
    Digitale Medien
    An electron microscopic and immunohistochemical study of Merkel cell carcinoma (1997)
    Springer
    Medical molecular morphology 30 (1997), S. 209-215 
    Details
    ISSN: 1860-1499
    Schlagwort(e): Merkel cell carcinoma ; Dense core granule ; Chromogranin A ; Electron microscopy ; Immunohistochemistry study
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract Two cases of Merkel cell carcinoma (MCC) were examined by electron microscopy and immunohistochemistry. Histologically, tumor cells, which extended from the dermis into the subcutis, showed anastomosing bands with partial trabecular pattern. The ultrastructural study showed tumor cells in case 1 with numerous neurosecretory granules. The number of granules in case 2, however, was smaller compared with that in case 1. Perinuclear bundles of filaments were present in case 2, but few bundles were observed in case 1. By immunohistochemistry, cytokeratin (CK)-8, -18, -19, and -20 and epithelial membrane antigen were stained positively within tumor cells in both cases. It was interesting that staining patterns of chromogranin A and of neuron-specific enolase were different in the two cases. These data indicated that CK-20 is a useful marker for diagnosing MCC and that ultrastructural and immunohistochemical differences in both cases were the result of phenotypic variation.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01545772
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  • 32
    Digitale Medien
    Digitale Medien
    Cervical cord ependymoma with numerous microrosettes (1997)
    Springer
    Brain tumor pathology 14 (1997), S. 145-151 
    Details
    ISSN: 1861-387X
    Schlagwort(e): Microrosette ependymoma ; Spinal cord ependymoma ; Trabecular architecture ; Immunohis-tochemistry ; Electron microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract “Microrosette ependymoma,” which is ependymoma with numerous microrosettes throughout the tumor, has rarely been reported. We describe an autopsy case of cervical cord ependymoma with two unusual features: the presence of numerous microrosettes and the formation of trabecular architecture. The tumor originated in the C2 segment of a man aged 23 years and gradually expanded over the following 15 years and 10 months until the entire cervical cord was involved. Beside the low grade of malignancy, the tumor cells exhibited a strong tendency to form microrosettes and trabecular architecture, which formed many perivascular pseudorosettes. The microrosettes mostly consisted of only two or a few more cells, in the absence of large rosettes. Thus the constituent cells were those forming perivascular pseudorosettes. Electron microscopy and immunohistochemistry characterized the ependymal properties of the microrosettes, whose lumina frequently contained fibril bundles similar to those of the Reissner's fiber fibrils, in addition to cilia and microvilli. The pathogenesis of the occurrence of numerous microrosettes is unknown; however, a defect in the mechanism of regulation of rosette formation and enlargement is the most likely explanation.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02478884
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  • 33
    Digitale Medien
    Digitale Medien
    Gastric carcinoid tumors in a brother-sister occurrence, with special reference to ultrastructural features of endocrine granules (1997)
    Springer
    Medical molecular morphology 30 (1997), S. 228-234 
    Details
    ISSN: 1860-1499
    Schlagwort(e): Gastric carcinoid tumors ; Familial occurrence ; Electron microscopy ; Endocrine granules
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract Our report concerns gastric carcinoid tumors in a man and his younger sister who had no endocrine symptoms. The tumors in both cases were multiple, located in the corpus, and showed no significant differences in light microscopic and immunohistochemical examination. In electron microscopic observation, the endocrine granules of tumor cells were roughly classified into four types: granules with fine granular content with low electron density, irregularly striped granules with medium electron density, concentrically laminated or regularly striped granules, or smaller and almost uniformly high electron-dense granules in which it was difficult to recognize the internal structure. From the sequential morphological changes of the granules, we have considered that each type of granule reflects various stages of granulogenesis, although the possibility that they are distinct and contain different hormones or chemical mediators is not absolutely ruled out.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01545775
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  • 34
    Digitale Medien
    Digitale Medien
    Ultrastructure of microvessels and tumor cell processes on central neurocytoma (1997)
    Springer
    Brain tumor pathology 14 (1997), S. 79-83 
    Details
    ISSN: 1861-387X
    Schlagwort(e): Neurocytoma ; Intraventricular tumor ; Electron microscopy ; Blood vessel ; Tumor cell process
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract A case of central neurocytoma that was confirmed with ultrastructural and immunohistochemical studies has been reported. Ultrastructurally, thin cytoplasmic processes of tumor cells showed differentiation into neuronal cells containing parallel bundles of microtubules and abortive synapses with dense-core vesicles and/or clear vesicles. It was frequently found that the clusters of tumor cell processes were close to or around the microvessels. Microvessels were composed of endothelial cells without fenestrations and had tight junctions in the endothelial clefts. Neurosecretory granules in thin cell processes appeared close to microvessels and may have been secreted around microvessels.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02478874
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  • 35
    Digitale Medien
    Digitale Medien
    Feinstrukturelle Untersuchungen über das Nuclear-Channel-System im menschlichen Endometrium (1977)
    Springer
    Archives of gynecology and obstetrics 223 (1977), S. 195-204 
    Details
    ISSN: 1432-0711
    Schlagwort(e): Electron microscopy ; Human endometrium ; Nucleus ; Nucleolus ; Channel system ; Elektronenmikroskopie ; Menschliches Endometrium ; Nukleus ; Nukleolus ; Channel-System
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Beschreibung / Inhaltsverzeichnis: Zusammenfassung Das Nuclear-Channel-System ist eine runde bis ellipsoide Struktur, deren Tubuli querangeschnitten oder längsangeschnitten in eine elektronendichte Matrix eingebettet sind. Das NCS liegt meist der Kernmembran dicht an, die in diesem Bereich Einziehungen aufweist. Gelegentlich ist ein Kontakt der Kernmembran mit der Membran eines Tubulus zu beobachten. Im NCS sind Partikel festzustellen, die den 150 Å großen Partikeln des Nucleolus vergleichbar sind, was auf einen Gehalt des NCS an RNA schließen läßt. In hier vorliegenden Untersuchungen konnte das NCS in Zellkernen endometrialer Drüsenzellen während der mittleren und späten Proliferationsphase beobachtet werden. Diese Befunde sprechen gegen eine zwingende Abhängigkeit des Auftretens des NCS vom Zeitpunkt der Ovulation. Das NCS kann somit nicht als eine für postovulatorisches Endometrium typische Struktur angesehen werden. Offensichtlich bestehen zwischen den hormonellen Faktoren und der Ausbildung des NCS Beziehungen, deren Bedeutung bis heute nicht sicher beurteilt werden kann.
    Notizen: Summary The Nuclear Channel System (NCS) is a distinct endometrial pattern as observed by transmission electron microscopy. The NCS was identified in the present study in cell nuclei of endometrial glands during mid and late proliferation. These observations do not support the concept that the NCS develops at the ovulation, and is therefore not a typical post ovulatory structure. It is suggested that there are relations between hormonal factors and the development of the NCS which are as yet no clarified.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00667389
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  • 36
    Digitale Medien
    Digitale Medien
    Ultrastrukturell-morphometrische Untersuchungen an Leberbiopsien — Der Einfluß von Ovulationshemmern auf die menschliche Leber (1977)
    Springer
    Archives of gynecology and obstetrics 223 (1977), S. 205-211 
    Details
    ISSN: 1432-0711
    Schlagwort(e): Electron microscopy ; Stereology ; Human liver ; Oral contraceptives ; Elektronenmikroskopie ; Stereologie ; Menschliche Leber ; Orale Kontrazeptiva
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Beschreibung / Inhaltsverzeichnis: Zusammenfassung Es wurden Leberbiopsien von 12 Frauen feinstrukturell-morphometrisch untersucht, die über einen Zeitraum von 2 Monaten bis zu 8 Jahren ein Kombinationspräparat zur hormonellen Antikonzeption eingenommen hatten. Die stereologischen Daten wurden bereits vorher erstellten Basiswerten für normale Lebern (Roessner et al., 1977) gegenübergestellt und auf signifikante Unterschiede hin überprüft (p〈0,001). Eine deutliche Volumenzunahme lysosomaler Zelleinschlüsse pro ml Hepatocyten und eine leichte Cytoplasmavergrößerung bei gleichzeitiger Verkleine-rung der Nuclei kennzeichnen die Veränderungen der Hepatocyten. Alle übrigen ermittelten Parameter lassen den Schluß zu, daß wesentliche Alterationen der Ultrastruktur der menschlichen Leber nach hormoneller Kontrazeption nicht auftreten. Ob damit auch funktionelle Alterationen auszuschließen sind, kann hier nicht beantwortet werden.
    Notizen: Summary Liver biopsies from 12 healthy women were investigated morphometrically — according to the methods of Weibel (1969). All women had taken an oral contraceptive agent during 2 til 76 months. The evaluated stereological datas were compared with base-line datas for normal human liver biopsies, which were elaborated in a previous investigation (Roessner et al., 1977). Statistical analysis was performed on an IBM 360/50 computer system, including F- and student's t-test. In the liver biopsy-specimen from the women, which were on oral contraceptives, the volume-density of lysosomes per ml hepatocytes shows a pronounced, significant (p〈0.001) increase. A moderate increase of cytoplasm is combined with decrease of nuclei-volume. The surface-densities of the rough and smooth endoplasmatic reticulum do not differ significantly from the datas calculated from normal human liver biopsies. All other datas too demonstrate that there are no striking changes in the ultrastructure of human liver, caused by oral contraceptives. Functional alterations however can not be excluded.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00667390
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  • 37
    Digitale Medien
    Digitale Medien
    The ultrastructure of granuloma annulare (1977)
    Springer
    Archives of dermatological research 259 (1977), S. 225-234 
    Details
    ISSN: 1432-069X
    Schlagwort(e): Granuloma annulare ; Necrobiosis of collagen ; Palisading granuloma ; Electron microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Beschreibung / Inhaltsverzeichnis: Zusammenfassung Neun Fälle von Granuloma annulare in verschiedenen Entwicklungsstadien wurden elektronenmikroskopisch untersucht, insbesondere Kollagenveränderungen, das entzündliche Infiltrat und Gefäßalterationen. Die nekrobiotischen Veränderungen des Kollagens bestehen in Auflockerung der Bündel vom Rande her, Schwellung der Fibrillen mit Verlust ihrer Querstreifung, völliger Desintegration des Kollagens und Niederschlägen von mucinösen Substanzen, Fibrin und Glykogen. In 2 Fällen waren sogenannte “filamentöse quergestreifte Aggregationen” (= sog. “long-spacing collagen”) nachweisbar. In späteren Stadien zeigte sich Kollagenneubildung. Im Infiltrat sind vor allem Histiocyten/Makrophagen mit Übergängen zu Epitheloidzellen und Riesenzellen, daneben auch Lymphocyten und später Neutrophile identifizierbar. Gefäßveränderungen bestehen in Endothelschwellung und Basalmembranverdoppelungen. Es besteht ein perivasculäres Infiltrat, jedoch keine echte Vaskulitis. Die Ergebnisse sprechen dafür, daß die Nekrobiose des Kollagens ohne primäre Vaskulitis ein initiales Ereignis bei Granuloma annulare darstellt. Pathogenetisch wird ein Immungeschehen vom verzögerten Typ diskutiert.
    Notizen: Summary Nine cases of granuloma annulare at different stages of development were investigated electron microscopically. Special attention was given to collagen changes, to the cellular infiltrate, and to vascular changes. The necrobiotic changes of the collagen were: loosening of the fibers at the borders of the bundles; swelling and loss of cross-striation of the fibrils; and complete degradation of the collagen, with precipitation of mucinous and fibrin material and of glycogen. In 2 cases “cross-banded filamentous aggregations” were present. In later stages new collagen synthesis occurred. In the infiltrate, histiocytes/macrophages were seen developing into epithelioid and giant cells. In addition, numerous active lymphocytes and, at later stages, some neutrophils were found. Vascular changes consisted of endothelial swelling and duplications of the basal lamina. A perivascular infiltrate was present, but no distinct vasculitis was seen. Our results indicate that the necrobiosis of the collagen without primary vasculitis may be an initial event in granuloma annulare. A delayed hypersensitivity mechanism is discussed with regard to pathogenesis.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00561450
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  • 38
    Digitale Medien
    Digitale Medien
    Chronic megakaryocytic-granulocytic myelosis — An electron microscopic study (1977)
    Springer
    Virchows Archiv 373 (1977), S. 191-211 
    Details
    ISSN: 1432-2307
    Schlagwort(e): Myeloproliferative disorder ; Thrombocytes ; Megakaryocytic-granulocytic myelosis ; Bone marrow biopsy ; Megakaryocyte lineage ; Electron microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary The fine structure of the bone marrow in chronic megakaryocytic-granulocytic myelosis (CMGM) was studied in 5 nontreated patients to investigate possible malignant proliferation of megakaryocytes and the role of megakaryopoiesis in fibrillogenesis, terminating in osteomyelofibrosis. In comparison with normal megakaryopoiesis there is an enormous increase of the megakaryocytic cell line and many immature and atypical forms are seen. Most conspicuous are microforms, nuclear-cytoplasmic disorganization and nuclear inclusions. Asynchrony of maturation causes abnormal thrombocytogenesis with premature detachment of platelets resulting in immature and peculiar giant forms of thrombocytes. Besides megakaryocytes appearing superficially normal the maturation anarchy of many cells is so severe that by analogy with observations in other leukaemic cells these abnormalities are thought to be representative of a malignant growth. Moreover, there is a striking accumulation of microfibrils and single collagen fibres around megakaryoblasts. Since these cells contain all those organelles commonly associated with fibre production the initial step for fibrillogenesis may therefore arise from the megakaryoblasts prior to platelet release, or any fibroblast proliferation.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00432237
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  • 39
    Digitale Medien
    Digitale Medien
    Immunohistochemical and electronmicroscopic observations on the local immune response in ulcerative colitis (1977)
    Springer
    Virchows Archiv 374 (1977), S. 271-273 
    Details
    ISSN: 1432-2307
    Schlagwort(e): Ulcerative colitis ; Immunoperoxidase ; Complement demonstration ; Electron microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary Inflammatory cell infiltrates in ulcerative colitis have been investigated by means of the immunoperoxidase method and by electronmicroscopy. Considerable morphological and functional changes of the local plasma cell population have been found. The absolute number of plasma cells is raised with a marked increase of IgG-cells and a relative decrease of IgA-cells. In particular complement (C3) has been demonstrated at the basement membrane of the surface epithelium and between epithelial cells. The significance of these findings, as a local humoral immune response, is briefly considered, with regard to their possible pathogenetic importance in aggravating and perpetuating the disease.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00432650
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  • 40
    Digitale Medien
    Digitale Medien
    Distribution of mucosubstances in adenoid cystic carcinoma (1977)
    Springer
    Virchows Archiv 375 (1977), S. 1-12 
    Details
    ISSN: 1432-2307
    Schlagwort(e): Adenoid cystic carcinoma ; Cylindroma ; Salivary glands ; Mucosubstances ; Cytochemistry ; Electron microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary The distribution of mucosubstances in adenoid cystic carcinoma was investigated, and an attempt was made to characterize histochemically the various mucosubstances present. For these purposes the high iron diamine technique (HID), as well as the Astra blue, aldehyde fuchsin and Alcian blue staining methods were employed. Alcian blue was further combined with the periodic acid-Schiff (PAS) technique, the Alcian blue being applied at pH levels between 0.5 and 2.5. In addition the effects of neuraminidase and hyaluronidase treatment as well as methylation and acid hydrolysis procedures on the staining qualities were studied. Acidic mucosubstances with varying histochemical properties were present in different structures of the neoplasm. The characteristic pseudocyst, a major structural component of the neoplasm, stained strongly with HID, Astra blue, aldehyde fuchsin and Alcian blue at low pH. These staining reactions were markedly suppressed by hyaluronidase treatment, and are apparently attributable to the presence of chondroitin 4- and/or 6-sulfate. Employing the Alcian blue-critical electrolyte concentration technique, the basophilia of the pseudocysts was suppressed at a concentration of 0.5–0.6 M MgCl2, which might indicate polysaccharides of relatively low degree of sulfation. An additional, non-sulfated acid mucin could also be demonstrated in these structures. In certain duct and gland like structures of the tumours, a change in staining pattern from blue or blue-red to red could be observed after exposure of the sections to neuraminidase and subsequent staining with the Alcian blue (pH 2.5)-PAS sequence. Similar observations were also made when the pH of the Alcian blue was lowered to 1.5–1.0, as well as after acid hydrolysis. These findings afford evidence for the presence of a neuraminidase susceptive sialomucin in certain epithelial secretions of the tumor. At the ultrastructural level the replicated basement lamina of the pseudocysts displayed a strong positive reaction with the PA-CrA-silver staining technique. Furthermore, amorphous material within the lumina of small duct like structures also displayed a positive reaction. The amorphous material of the cystic compartments was less reactive.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00430640
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  • 41
    Digitale Medien
    Digitale Medien
    Esthesioneuroblastoma (1977)
    Springer
    Virchows Archiv 375 (1977), S. 123-128 
    Details
    ISSN: 1432-2307
    Schlagwort(e): Esthesioneuroblastoma ; Light microscopy ; Electron microscopy ; Argyrophil reaction ; Formaldehyde-induced fluorescence
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary A case of esthesioneuroblastoma was examined by histological, histochemical and electron microscopic techniques. The tumour cells showed an argyrophil reaction with the Grimelius technique and contained cytoplasmic secretory granules, but in contrast to previous reports were devoid of histochemically demonstrable biogenic amines. For routine diagnosis the argyrophil technique may be useful in differentiating this type of tumour from epidermoid carcinoma.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00428100
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  • 42
    Digitale Medien
    Digitale Medien
    Target-targetoid phenomenon of the human muscle fibers (1977)
    Springer
    Virchows Archiv 375 (1977), S. 13-22 
    Details
    ISSN: 1432-2307
    Schlagwort(e): Target-targetoid phenomenon ; Histochemistry ; Electron microscopy ; Z-band alteration ; Incomplete innervation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary Target and targetoid fibers in a muscle biopsy from a patient with paralysis of the deltoid and supraspinatus muscles were studied by light and electron microscopy. The probable cause of the neuropathy was tumor compression. Target and targetoid change was exclusively confined to hypertrophic or normal-sized fibers. Morphometric evaluation of the target and targetoid fibers showed no significant difference between them. With the electron microscope, up to 4 structural zones were seen in the typical target fiber but many were devoid of either zone 2 (halo) or zone 3, or both. It was conceivable that focal irregularity and streaming of Z-bands were the primary alterations in the process of target-targetoid fiber formation, and that this phenomenon was induced both by partial residual innervation as well as re-innervation.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00430641
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  • 43
    Digitale Medien
    Digitale Medien
    Spontaneous atherosclerosis: An ultrastructural study in the White Carneau pigeon (1977)
    Springer
    Virchows Archiv 375 (1977), S. 147-157 
    Details
    ISSN: 1432-2307
    Schlagwort(e): Electron microscopy ; Atherosclerosis ; Pigeon
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary The atherosclerotic lesions, associated with the celiac intimal smooth muscle cushions, of four and five year old White Carneau pigeons were studied with the light and electron miscroscopes. Light microscopic examination of the spontaneous lesions demonstrated large intimal cushions composed of smooth muscle, abundant collagen, clusters of foam cells and cholesterol crystal clefts. Ultrastructural examination of the intimal atheroma revealed dilatations between apposing endothelial cells which contained a flocculent material, similar to that seen in the subendothelial space. The subendothelial compartment contained abundant collagen, extracellular lipid, vesiculated material and cell processes which contained a flocculent matrix and tubular-like elements. In addition, fibroblast-like interlaminar cells were often observed. Numerous intimal smooth muscle cells were seen which displayed varied morphology. Abundant foam cells were also present within the intimal atheromas. The presence of atherosclerotic lesions in preexisting intimal smooth muscle cushions suggests that hemodynamic factors may be important in the progression of these spontaneous lesions. Endothelial cell dilatations may provide an important route of transport for circulating elements which may accumulate within the subendothelial space. Morphologically, it appears that the smooth muscle cells undergo modification and may represent the precursors of foam cells in this species.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00428102
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  • 44
    Digitale Medien
    Digitale Medien
    Ultrastructure of the liver in a case of childhood cystinosis (1977)
    Springer
    Virchows Archiv 377 (1977), S. 43-48 
    Details
    ISSN: 1432-2307
    Schlagwort(e): Childhood cystinosis ; Liver ; Electron microscopy ; Dark cells ; Tight junctions
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary Ultrastructural findings in the liver in a case of childhood cystinosis are reported. Crystalline structures were found mainly in Kupffer cells. The presence of dark cells, with or without crystals, was the most striking feature observed. Such cells have already been noted within the kidney on one occasion when it was shown that the dark substance was L-cystine (Spear et al., 1971). In this case identical dark material was also found extracellularly. The data shows that free cystine can fill cell cytoplasm and extracellular spaces and the possibility that cystine overproduction may take place in the hyaloplasm should be considered. Extracellular location of cystine in the tubules might account for an increase in epithelial permeability and thus for the Fanconi syndrome.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00432697
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  • 45
    Digitale Medien
    Digitale Medien
    Sporulation and spore liberation in Aspergillus fumigatus (1977)
    Springer
    Mycopathologia 60 (1977), S. 175-177 
    Details
    ISSN: 1573-0832
    Schlagwort(e): Aspergillus fumigatus ; Spore formation ; Electron microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00448412
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  • 46
    Digitale Medien
    Digitale Medien
    An ultrastructural survey of the genus Prototheca with special reference to plastids (1977)
    Springer
    Mycopathologia 61 (1977), S. 117-119 
    Details
    ISSN: 1573-0832
    Schlagwort(e): Prototheca ; Colorless alga ; Plastids ; Electron microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Abstract An ultrastructural investigation of six different species of Prototheca showed that all of them contained starch grains enclosed in double-membrane-bounded structures recognized as plastids. It is concluded that these unicellular species of Prototheca must be considered as non-photosynthetic algae.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00443840
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  • 47
    Digitale Medien
    Digitale Medien
    The surface ultrastructure of the habenular complex of the rat (1977)
    Springer
    Anatomy and embryology 152 (1977), S. 43-64 
    Details
    ISSN: 1432-0568
    Schlagwort(e): Habenula ; Ependyma ; Supraependymal cells ; Supraependymal axons ; Electron microscopy ; Rat
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary The surface features of the ependymal lining of the habenular complex in rats, aged between three weeks and nine months, were studied by means of scanning and transmission electron microscopy. The ependyma of the medial habenular nucleus is heavily ciliated, the cilia obscuring underlying substructure in SEM — preparations. On the habenular commissure most cilia are arranged in tufts. Cilia are provided with segmental indentations and occasional apical thickenings. Vesicular protrusions of the ependymal cytoplasm into the ventricular lumen and the frequent occurrence of homogeneous supraependymal globules were interpreted as signs of ependymosecretory activity of nucl. hab. med. Supraependymal cells are most numerous on the anterior and superior surface of the habenular commissure. Cells presenting features identical to Kolmer (epiplexus) cells were identified on the ventricular surface of nucl. hab. med. in one specimen showing degenerative changes of undetermined aetiology in the habenular nuclei. It is therefore suggested that such cells need not necessarily be restricted to the choroid plexus. Supraependymal unmyelinated axons are particularly numerous on both nucl. hab. med. and commiss. hab. They make desmosome contacts (maculae adherentes) with the ependymal plasmalemma. Contacts presenting all features of typical synapses were not encountered. The vesicle population of the axonal profiles mainly comprises 35–50 nm translucent round vesicles besides small numbers of 60–100 nm dense-cored vesicles and large pleiomorphic vesicles. Most probably the axons belong to the well-established dense population of serotonergic axons in the dorsal part of the third ventricle.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00341434
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  • 48
    Digitale Medien
    Digitale Medien
    An electron microscopic study of the developing caudate nucleus in euthyroid and hypothyroid states (1977)
    Springer
    Anatomy and embryology 150 (1977), S. 335-364 
    Details
    ISSN: 1432-0568
    Schlagwort(e): Caudate nucleus ; Hypothyroidism ; Development ; Electron microscopy ; synaptogenesis
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary Thyroid hormone exerts a powerful influence on CNS growth and maturation. Hypothyroidism early in life has long been known to cause disturbances in innate behavior, motor performance, severe and frequently irreversible mental retardation. In this deficiency, depressed caudate neurogenesis, cell migration and neuropil development during the rapid period of CNS growth may contribute to the clinical picture of perceptual handicaps often seen in cretins. Light microscopic and Golgi studies of the developing caudate nucleus in thyroid deficiency have been carried out to help attain insights into the mechanisms whereby the extrapyramidal system regulates motor function. The ultrastructural study of caudate nuclear cytogenesis and synaptogenesis in normal and hypothyroid states provides more detailed information for further analysis of the problem. Hypothyroidism was induced from birth by adding propylthiouracil to the food and drinking water of lactating dams. Linear development of the caudate nucleus of both normal and hypothyroid rats at ages 8, 14, 20, 30 and 42 days was studied by electron microscopy. Thyroid glands were examined by light microscopy to assess the normal and deficient states. Immature cells, primitive processes and synapses were the characteristic features of the 8-day-old normal caudate nucleus. Distinctively wide cisternae of the rough endoplasmic reticulum, loosely packed Golgi apparatus and chromatin clumps throughout the nuclei of the neurons were significant early morphologic variations. The dramatic cytoarchitectural maturation in the 14- and 20-day normal caudate neuropil points to the rapidity of developmental rate. After the growth spurt of the first three weeks a maturational plateau occurs which is characterized by well-formed neuronal cytoplasmic organelles, myelinated and non-myelinated axons, axon terminals, dendrites and their spines, and synapses. Thyroid deficiency causes a marked maturational delay of approximately 7 days in caudate neuronal proliferation, the elaboration of neuronal networks and the attainment of mature synaptic contents and membranes. This delay is evidenced by comparison of the structural similarities between 8-day-old normal and 14-day-old deficient rats; and additional comparisons between the 14-day-old normal and 20-day-old hypothyroid rats. A rapid “catch up” process in fine structural morphogenesis takes place in the period between days 14 and 30 in the deficient animals. Repression of thyroid function does not entirely prevent development of the caudate nucleus but allows a fairly extensive, though critically incomplete degree of maturation. This imperfection is manifested by a decrease in the number of synaptic contacts that persists even after the rapid “catch up” phenomenon of caudate synaptogenesis.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00318351
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  • 49
    Digitale Medien
    Digitale Medien
    Interaction between mesenchymal cells and the posterior iris epithelium in chicken embryos (1977)
    Springer
    Anatomy and embryology 151 (1977), S. 109-118 
    Details
    ISSN: 1432-0568
    Schlagwort(e): Iris ; Mesenchymal cells ; Chicken embryo ; Electron microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary The iris anlage of 3–10 day old chicken embryos was studied by both light and electron microscopy. Serial semithin sections showed that some of the mesenchymal cells overlying the eye cup moved into the primitive eye cavity by the 3rd day of incubation. On the 4th day some of these cells came into close contact with the basement membrane of the dorsal iris epithelium. The bases of the epithelial cells were flat at this stage. Towards the 10th day they formed cytoplasmic processes which did not penetrate the basement membrane. Fine mesenchymal cytoplasmic processes and a large number of extracellular fibrils developed in the epithelial-mesenchymal interface. The fine mesenchymal processes came into close contact with the basement membrane of the posterior iris epithelium but did not penetrate it. Collagen-like material was observed within the cisternae of the rough ER of the mesenchymal cells at certain stages of development. Both, the mesenchymal cells and the collagen fibrils adjacent to the posterior iris layer disappeared by the 10th day when the entire iris epithelium was completely pigmented. The possible origin of the collagen fibrils and the differentiation of the posterior iris epithelium are discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00315303
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  • 50
    Digitale Medien
    Digitale Medien
    Primary 9+0 cilia in the embryonic and the adult human heart (1977)
    Springer
    Anatomy and embryology 151 (1977), S. 127-139 
    Details
    ISSN: 1432-0568
    Schlagwort(e): Cilia ; Human heart ; Electron microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary Primary 9+0 cilia have been found in the embryonic and the adult human heart. Proximally the cilia show the typical (+0 filament arrangement. Rearrangement of the filaments occur in their distal regions. The cilia are usually found in deep invaginations of the cell membrane, but can also be found in a superficial position. Close connections are frequently found between cilia and thin cytoplasmic extensions from neighbouring cells.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00297476
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  • 51
    Digitale Medien
    Digitale Medien
    Placodes of the chick embryo studied by SEM (1977)
    Springer
    Anatomy and embryology 151 (1977), S. 97-108 
    Details
    ISSN: 1432-0568
    Schlagwort(e): Chick embryo ; Placode ; Electron microscopy ; Invagination ; Midbody
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary The otic, the lens and the nasal placodes have been examined in chick embryos between stages 10 and 18 of Hamburger and Hamilton. At the stage when each placode first becomes visible conspicuous differences have been seen in the surface morphology between those cells which will invaginate and form the placode and those which will remain on the surface of the head, forming the epidermis. The differences become more pronounced with increasing development. The placode cells possess many surface projections whilst the epidermal cells do not. These differences in surface morphology are related to other differences which are visible in TEM sections, the placode cells being highly columnar and extending the full depth of the placode, whilst the epidermal cells are cuboidal or even squamous. This modification in cell shape of the placode cells is correlated with the presence of longitudinally orientated microtubules. The mechanism of invagination is discussed and evidence is presented which supports the idea that there is a migration of cells into the placode from one side. Such a phenomenon would help to explain the asymmetrical structure of the placode, including the presence of the overhanging lip.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00315302
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  • 52
    Digitale Medien
    Digitale Medien
    Electron microscopical study of psoriasis pustulosa (1977)
    Springer
    Archives of dermatological research 260 (1977), S. 167-178 
    Details
    ISSN: 1432-069X
    Schlagwort(e): Electron microscopy ; Psoriasis pustulosa ; Spongiform pustule
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Beschreibung / Inhaltsverzeichnis: Zusammenfassung Fünf Fälle von Psoriasis pustulosa wurden elektronenmikroskopisch untersucht. Die Hauptveränderungen im Corium bestanden in Capillardilatation mit intravasculären polymorphonukleären Leukocyten und Erythrocyten. Leukocyten traten durch die vasculären Spalten und Fenestrationen und wanderten durch das deutlich ödematöse Corium zur Epidermis. Lymphgefäße fanden sich ebenfalls. Histiocyten mit Langerhansgranula wurden in der Dermis und Epidermis beobachtet. Die morphologischen Veränderungen in der Epidermis waren abhängig von der Lokalisation und besonders stark ausgeprägt in der Nähe von voll entwickelten Pusteln in höheren Zellschichten. Früher beschriebene Befunde konnten bestätigt werden. Perinukleäre Cytolyse und andere Veränderungen in den Keratinocyten scheinen sekundär aufgrund des Ödems und der Ansammlung von Neutrophilen innerhalb der Epidermis zu entstehen. Immunologische Phänomene könnten hierfür verantwortlich sein.
    Notizen: Summary Five cases of psoriasis pustulosa were examined by electron microscopy. The main features within the dermis were dilated capillaries filled with polymorphonuclear leukocytes and red cells. Neutrophils passed out of the vessels through the gaps and fenestrations and migrated towards the epidermis throughout the distinctly edematous corium. Lymphatic vessels could be found. Histiocytes containing Langerhans granules were observed in the dermis and epidermis. The morphological changes of the epidermis depented on the place examined and were most evident near fully developed pustules in the upper layers. The presence of previously described morphological findings could be confirmed. Perinuclear cytolysis and other changes in the keratinocytes, however, seem to be secondary, resulting from edema and accumulation of neutrophils within the epidermis. Immunological phenomenona may be responsible for the latter event.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00561411
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  • 53
    Digitale Medien
    Digitale Medien
    Some histological aspects of amyloid polyneuropathy (1977)
    Springer
    Acta neuropathologica 37 (1977), S. 119-125 
    Details
    ISSN: 1432-0533
    Schlagwort(e): Amyloid polyneuropathy ; Electron microscopy ; Nerve fibers abnormalities ; Giant axons
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary Two sporadic cases of amyloid polyneuropathy with clinical features corresponding to the Portuguese type of this disease were studied. Histological examination of sural nerve demonstrated a marked loss of myelinated and unmyelinated fibers in the case 1 due to axonal degeneration, high content of fibers with segmental demyelination and the occurrence of several enlarged axons filled with the 10 nm filaments (so-called giant axons). In the case 2 there was total loss of unmyelinated axons and myelinated fibers were nearly completely lacking. In the development of changes in the myelinated fibers their direct compression by amyloid deposits seems to play an important role. It leads to the appearance of both axonal degeneration and segmental demyelination. The latter seems to be due to local compression and it may involve many fibers. In the light of observations reported by other authors the mechanism of changes developing in unmyelinated fibers is explained by the presence of changes in the cells of posterior root ganglia, however the question whether some abnormalities seen in unmyelinated axons could not be related to the pressure exerted by amyloid deposits directly to these fibers, remains open.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00692057
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  • 54
    Digitale Medien
    Digitale Medien
    Filamentous and multilamellated cytoplasmic inclusions in progressive multifocal leukoencephalopathy (1977)
    Springer
    Acta neuropathologica 37 (1977), S. 263-265 
    Details
    ISSN: 1432-0533
    Schlagwort(e): Progressive multifocal leukoencephalopathy ; Multiple sclerosis ; Demyelination ; Electron microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary Inclusions identical to those described in multiple sclerosis (MS) brain by Prineas (1975) have been seen by ultrastructural study of cerebral tissue in two elsewhere reported cases of Progressive Multifocal Leukoencephalopathy (PML). The meaning of these formations, which have been suggested to be related to a special process of demyelination in MS, is discussed in the light of the hypotheses concerning the demyelination mechanism in PML. Whatever might be their significance, these cytoplasmic non viral inclusions are not specific for MS.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00686889
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  • 55
    Digitale Medien
    Digitale Medien
    Clofibrate-induced myopathy in the rat (1977)
    Springer
    Acta neuropathologica 39 (1977), S. 135-138 
    Details
    ISSN: 1432-0533
    Schlagwort(e): Clofibrate ; Striated muscle ; Histochemistry ; Electron microscopy ; Experimental animal
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary Rats were given daily injections of 0.2 or 0.5 g/kg chlorophenoxyisobutyrate (clofibrate) for 9 to 46 days. Lower leg muscles were studied with light and electron microscopy. Daily treatment with 0.5 g/kg of the drug for 26–46 days caused myopathic changes.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00703319
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  • 56
    Digitale Medien
    Digitale Medien
    Focal mucoid degeneration of peripheral nerve (1977)
    Springer
    Acta neuropathologica 37 (1977), S. 69-72 
    Details
    ISSN: 1432-0533
    Schlagwort(e): Neuropathy ; Fibroblast ; Endoneurial edema ; Peripheral nerve ; Electron microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary “Focal mucoid degeneration” was found in a N. suralis biopsy of a 8 year old child, diagnosed clinically and electrophysiologically as progressive muscular atrophy Charcot-Marie-Tooth.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00684543
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  • 57
    Digitale Medien
    Digitale Medien
    The ultrastructure of early visna lesions (1977)
    Springer
    Acta neuropathologica 37 (1977), S. 127-135 
    Details
    ISSN: 1432-0533
    Schlagwort(e): Slow infection ; Viral encephalomyelitis ; Electron microscopy ; C-Type virus ; Immunopathology
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary The ultrastructure of visna, a slowly progressive meningo-encephalomyelitis of sheep, was studied in animals sacrificed one month after intracerebral inoculation of visna virus. The major pathological changes, representative of those seen during the first year after infection, consist of inflammation and minor focal destructive lesions of grey and white matter. The inflammatory infiltrates, both subependymal and perivascular as well as of the choroid plexus, were composed mainly of lymphocytes and macrophages with varying numbers of plasma cells. The demyelination seen was of the secondary or Wallerian type. There was no evidence of primary demyelination. Visna virions were not seen in any of the CNS material studied. The ultrastructural findings are compatible with the view that lesions in visna may be induced by a cell-mediated immune response. However, changes characteristic of an autoimmune reaction to myelin antigens were not observed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00692058
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  • 58
    Digitale Medien
    Digitale Medien
    Etude en microscopie électronique des lésions nerveuses, musculaires et cutanées déterminées par le maléate de perhexiline (1977)
    Springer
    Acta neuropathologica 38 (1977), S. 53-59 
    Details
    ISSN: 1432-0533
    Schlagwort(e): Perexiline maleate ; Drug-induced neuropathy ; Nerve biopsy ; Muscle biopsy ; Skin biopsy ; Electron microscopy ; Lysosomes ; Segmental demyelination
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary The pathological findings in four nerves and muscles and in one skin biopsies from four patients treated with perhexiline maleate for angina pectoris are reported. In every case, a muscular denervation atrophy and a decrease in the large diameter myelinated fibers were observed. Only one case showed a decrease of the total number of myelinated fibers, on quantitative studies. The electron microscopic study of each nerve displayed findings consistent with a predominant schwannian degeneration, associated with a few onion bulbs formations and, in two cases, with a mild wallerian degeneration. The most striking finding consisted in the presence of polymorphous membrane-bound inclusions reminding the morphology of lysosomal complex lipids. These structures were very abundant in Schwann cells, but they were seen also in fibrocytes, endothelial and pericytic cells. Similar inclusions were present in the single muscle and skin biopsies studied by electron microscopy. In the muscle, they were seen in muscular cells as well as in endothelial and pericytic cells. In the skin, similar inclusions were observed in endothelial, smooth muscle and sweat gland cells. These inclusions were difficult to identifiy in one micron thick sections, emphazing the need of ultrastructural study for diagnostic purposes.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00691277
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  • 59
    Digitale Medien
    Digitale Medien
    Pituitary growth hormone cell adenoma with cytoplasmic tubular aggregates in the capillary endothelium (1977)
    Springer
    Acta neuropathologica 37 (1977), S. 77-79 
    Details
    ISSN: 1432-0533
    Schlagwort(e): Cytoplasmic tubular aggregates ; Electron microscopy ; Growth hormone cells ; Pituitary adenoma
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary Electron microscopy revealed the presence of cytoplasmic tubular aggregates in the capillary endothelium of a sparsely granulated growth hormone cell adenoma removed surgically from a 25-year-old female patient with acromegaly. To our knowledge, this is the second publication describing these structures in hypophysial growth hormone cell adenomas.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00684545
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  • 60
    Digitale Medien
    Digitale Medien
    Ultrastructural and biochemical studies on ouabain-induced oedematous brain (1977)
    Springer
    Acta neuropathologica 37 (1977), S. 95-100 
    Details
    ISSN: 1432-0533
    Schlagwort(e): Brain oedema ; Ouabain ; Na-K-ATPase ; Na-ion ; Electron microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary Ouabain, a specific inhibitor of active cation transport across cell membrane, was applied topically to brain surface of cats. The cerebral cortex to which 10−3 M ouabain was applied showed a spongy state, which was ultrastructurally revealed to be swelling of the neuronal cell processes, especially of dendrites. Astrocytes did not show swelling even in the most severely affected lesions. There was a marked increase in sodium with a slight increase of water in the most severely affected lesions. There was a marked increase in sodium with a slight increase of water in the cortex treated with 10−3 M ouabain. No evidence of increased vascular permeability was noted in the cortex, morphologically or biochemically. Electron microscopic cytochemistry to detect the sodium ion revealed that the increased sodium in the cortex accumulated in the swollen neuronal cell processes. It is speculated that neuronal rather than astrocytic elements might be highly dependent upon active cation transport, and that intracellular oedema in the brain tissue responsible for impairment of active cation transport might be related primarily to neuronal elements.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00692054
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  • 61
    Digitale Medien
    Digitale Medien
    Hypertrophie des T-Systems in Targetfasern (1977)
    Springer
    Acta neuropathologica 38 (1977), S. 39-43 
    Details
    ISSN: 1432-0533
    Schlagwort(e): Amyotrophic lateral sclerosis ; Muscle biopsy ; Target fibre ; Lamellar inclusions ; Transversal tubular system ; Electron microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary In a muscle biopsy from a patient suffering about 2 years from an amyotrophic lateral sclerosis groups of atrophic fibres and target fibres were observed. By electron microscopic examination, amongst numerous parallel or spinal cisternae near the plasma membrane, single-membrane bound smooth tubular elements with a constant diameter of 200–300 Å occur in the central and intermediary zone of some target fibres. The connexion between these tubules and the terminal cisternae (triads) of the sarcoplasmic reticulum, suggest that this phenomenon seems to be a proliferation of the transversal-tubular system due to the partial denervation. Only a mechanical displacement of the T-tubules as a result of the destruction of the muscle fibres is not plausible, because the T-system volumetrically amounts only 0.3–0.5% of the muscle fibre.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00691274
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  • 62
    Digitale Medien
    Digitale Medien
    Extraocular muscles: Morphogenetic study in humans light microscopy and ultrastructural features (1977)
    Springer
    Acta neuropathologica 38 (1977), S. 87-93 
    Details
    ISSN: 1432-0533
    Schlagwort(e): Human fetal muscle ; Electron microscopy ; Motor end-plate ; Extraocular muscles
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary The development of human extraocular muscles (EOM) was studied in a series of fetal specimens (12–24 weeks gestation). EOM were evaluated by enzyme histochemistry (EZ) (NADH and ATPase), by differential phase contrast microscopy (DPC) and electron microscopy (EM). In the early fetus (14 weeks), there was no clear-cut sub-division into fibre types. A uniform histochemical reaction was seen with NADH while ATPase showed light and dark myotubes. Myotubes contained large central nuclei, prominent eccentric nucleoli, abundant glycogen granules, free ribosomes, numerous mitochondria, and dense and looser bundles of myofilaments. Mesenchymal cells undergoing mitosis and fibroblasts with prominent stacks of rough endoplasmic reticulum were scattered within endomysium. Mast cells with well formed cytoplasmic granules were found as early as 18–24 weeks. The same specimens by DPC showed differentiation into at least 4 different fibre types at 12 weeks. All the intramuscular nerves at 12–16 weeks were composed of unmyelinated fibres. At 18 weeks, myelinated axons were present. Morphologically immature end-plates devoid of junctional folds were found at 12 weeks. The motor innervation of some EOM appears to be derived from more than one axon (multiple innervated fibres). At 18 weeks gestational age, differentiation into fibre types became apparent by enzyme histochemistry. These histochemical and morphological findings suggest that morphologically mature endplates are not prerequisites for differentiation into muscle fibre types.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00688553
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  • 63
    Digitale Medien
    Digitale Medien
    Electron microscopic features of the resting microglia in the rabbit hippocampus, identified by silver carbonate staining (1977)
    Springer
    Acta neuropathologica 38 (1977), S. 195-201 
    Details
    ISSN: 1432-0533
    Schlagwort(e): Resting microglia ; Silver impregnation ; Electron microscopy ; Hematogenous cell
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary Vibratome sections of hippocampus of adult rabbits were stained by a modified Hortega's silver-carbonate method. Impregnated materials were examined by electron microscopy to decide fine-structural characteristics of the resting microglia. Comparing their characteristics with those of macrophages, we came to the following conclusions:(1) Impregnated resting microglia in the hippocampus of adult rabbits can be identified as cells having distinct fine structures. (2) Resting microglia are morphologically different from macrophages or their precursor cells, and therefore, seem not to be hematogenous cells sojourning in the normal brain parenchyma.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00688065
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  • 64
    Digitale Medien
    Digitale Medien
    Infection of cultivated CNS tissue with herpes virus, HSVII a reappraisal (1977)
    Springer
    Acta neuropathologica 39 (1977), S. 177-180 
    Details
    ISSN: 1432-0533
    Schlagwort(e): Herpes simplex virus type II ; Organized CNS culture ; Cerebellar explants ; Electron microscopy ; Herpes infection
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary Organized cultures of newborn rat and hamster cerebellum were infected with herpes virus type II, after 7 and 14 days “in vitro”. 48 h after the infection, electron microscopic examination of the cultures showed that astrocytes contained numerous intranuclear and intracytoplasmic viral particles, while neurons remained apparently intact. The specificity of the infection for a given cell type is discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00703326
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  • 65
    Digitale Medien
    Digitale Medien
    Paraplegia due to actinomyces viscosus infection in a cat (1977)
    Springer
    Acta neuropathologica 39 (1977), S. 231-235 
    Details
    ISSN: 1432-0533
    Schlagwort(e): Actinomyces viscosus ; Epidural space ; Spinal cord ; Cat ; Electron microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary In a 3 year old female domestic cat a suppurative, granulomatous lesion of the tail and sacral area penetrated into the epidural space, causing paraplegia. A. viscosus was isolated from the inflammatory tissues. A comparative light and electron-microscopic study of the bacterial elements and the architecture of the granules (Drusen) show that the latter are in-vivo microcolonies of the agent.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00691702
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  • 66
    Digitale Medien
    Digitale Medien
    Comparative ultrastructural observations on peripheral nerve abnormalities in the late infantile, juvenile and late onset forms of metachromatic leukodystrophy (1977)
    Springer
    Acta neuropathologica 39 (1977), S. 237-245 
    Details
    ISSN: 1432-0533
    Schlagwort(e): Metachromatic leukodystrophy ; Peripheral nerve ; Electron microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary The ultrastructural findings in nerve biopsies from two cases of late onset metachromatic leukodystrophy were compared with those in cases of late infantile and juvenile onset. Hypertrophic changes and regenerating clusters were more evident in the late onset cases, in which macrophages were less frequent, presumably reflecting the chronicity of the disorder in this form. Inclusions within Schwann cells and endoneurial macrophages were similar in all four cases. Myelin figures, in which the periodicity of major dense lines was 8 nm, were present in Schwann cells associated with myelinated axons. The electron lucent zones between the major dense lines were bisected by lines of lesser electron density. These inclusions were probably related to myelin breakdown. All other inclusions displayed a periodicity of 5.8 nm and consisted of zebra bodies, vacuoles containing irregularly orientated lamellar material and stacks of flattened discs. These inclusions represented the metachromatic sulphatide deposits. Occasional inclusion bodies were observed within axons.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00691703
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  • 67
    Digitale Medien
    Digitale Medien
    An ultrastructural study of developing cerebral infarction following bilateral carotid artery occlusion in spontaneously hypertensive rats (1977)
    Springer
    Acta neuropathologica 40 (1977), S. 171-177 
    Details
    ISSN: 1432-0533
    Schlagwort(e): Spontaneously hypertensive rats ; Bilateral carotid occlusion ; Acute ischemic brain damage ; Electron microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary An ultrastructural study of cerebral infarcts in spontaneously hypertensive rats 1–5 h after bilateral carotid artery occlusion was performed. The alterations of the neocortex consisted of shrinkage of the neurons surrounded by swollen astrocytic processes. Distension of the rough endoplasmic reticulum of the neuronal cytoplasm appeared early, while changes of the mitochondria were slight. Though there appeared slight to moderate perivascular astrocytic swelling, endothelial swelling was rare and there was no severe narrowing of the capillary lumen. There were no filling defects of colloidal carbon injected to the blood vessels of the ischemic brains. Ischemic neuronal alterations were proved to develop in the absence of severe morphological changes of the microvasculature in the developing cerebral infarcts in the present experimental model.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00688707
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  • 68
    Digitale Medien
    Digitale Medien
    Experimental vaccinia virus meningoencephalitis in adult albino mice: Virological, light microscopic and ultrastructural studies (1977)
    Springer
    Acta neuropathologica 40 (1977), S. 193-205 
    Details
    ISSN: 1432-0533
    Schlagwort(e): Poxvirus group ; Vaccinia virus, neurovirulent ; Neurovaccinia strains ; Meningoencephalitis, experimental ; Virus morphogenesis ; Electron microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary Adult NMRI mice were inoculated intracerebrally with 1.5–5×102 pfu of the “neurovirulent” strain Ma1 of vaccinia virus. The animals usually became diseased on day 3 and died on day 5 or 6 p.i. Infectivity assay studies on the mouse brains yielded a steeply rising viral titer comparable with that exhibited by several other so-called neurovaccinia strains after intracerebral inoculation. Histological studies revealed a patchy leptomeningitis most apparent over the basal brain surface. Mononuclear elements of varied size were the most common infiltrate cells in the meningitic lesions. These often showed a pronounced hemorrhagic and necrotizing character. In some places, the cellular infiltrates within the subarachnoid space extended along the perforating vessels into the superficial cerebral cortex. By electron microscopy, all essential stages of virus morphogenesis including the discharge of mature virions could be seen in numerous mononuclear phagocytes and adventitial cells. In contrast to this, no unequivocal neuroectodermal cell showed morphologic evidence of productive viral infection. Likewise, no endothelia or vascular myocytes were encountered which contained newly formed virus particles. However, alterations of the walls of blood vessels and signs of disturbed vascular permeability were a frequent finding in the meningeal foci of inflammation as well as in and around the track left by the inoculating needle. Another ultrastructural feature of the meningitic lesions was the widespread occurrence of degenerating or disintegrating infiltrate cells both infected and uninfected ones. The virologic and morphologic findings observed in this model of experimental vaccinia virus meningo-encephalitis are discussed with regard to data previously published by other authors. The propensity of strain Ma1 of vaccinia virus (and most probably of other so-called neurovaccinia strains) for replication in mesenchymal cells in particularly emphasized.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00691954
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  • 69
    Digitale Medien
    Digitale Medien
    Ultrastructural localisation of sterols in ethylnitrosourea-induced schwannomas (1977)
    Springer
    Acta neuropathologica 40 (1977), S. 73-77 
    Details
    ISSN: 1432-0533
    Schlagwort(e): Transplacental carcinogenesis ; Ethylnitrosourea ; Schwannomas ; Free cholesterol ; Electron microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary Malignant schwannomas of the trigeminal nerves were induced transplacentally by a single intravenous injection of 30 mg of N-ethyl-N-nitrosourea per kg of body weight into pregnant BD-IX rats on the 15th day of gestation. These neoplasms consisted of a pleomorphic population of Schwann cells and occasional fibroblasts. In order to preserve sterols the tumours were fixed in a digitonin-containing aldehyde solution. The electron microscope investigation of the tissues so treated revealed various structures: lamellar, tubular and complex formations were all observed. In addition, the unit membranes, including those of various cytoplasmic organelles, showed areas of increased contrast. The abundance of these fine structural configurations indicated the high sterol content of ethyl-nitrosourea-induced schwannomas.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00688575
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  • 70
    Digitale Medien
    Digitale Medien
    Ultrastructure of muscle and sensory nerve in Fabry's disease (1977)
    Springer
    Acta neuropathologica 38 (1977), S. 187-194 
    Details
    ISSN: 1432-0533
    Schlagwort(e): Fabry's disease ; Muscle biopsy ; Nerve biopsy ; Electron microscopy ; Lamellar bodies
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary A study of a muscle biopsy has provided evidence of storage involvement of the skeletal muscle fibres in Fabry's disease. In the endothelial cells of the capillaries, the inclusions were more abundant and pleomorphic. Muscle satellite cells were spared. In the sensory nerve biopsy, the perineurial and endothelial cells contained lamellar bodies, but not the Schwann cells. There was a slight reduction of the number of the small myelinated fibres and of the unmyelinated fibres. An electron microscopic study of a muscle biopsy can be helpful in the diagnosis of the disease. The physiopathology of the pain attacks is still a matter for discussion.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00688064
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  • 71
    Digitale Medien
    Digitale Medien
    Hereditary “Cavitating” leucodystrophy in Dalmatian dogs (1977)
    Springer
    Acta neuropathologica 40 (1977), S. 163-169 
    Details
    ISSN: 1432-0533
    Schlagwort(e): Leucodystrophy ; Myelin ; Dog ; Histochemistry ; Electron microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary A recently recognised progressive disorder of central myelin in Dalmatian dogs is described. The disease is probably transmitted by autosomal recessive inheritance. The symptoms started at the age of 3–6 months and were dominated by visual deficiency and/or progressive locomotive abnormalities. Pathologically, the most consistent lesions were found bilaterally in the region of the centrum semiovale. The optic nerves were also frequently affected, the basal ganglia and spinal cord were affected infrequently. The main lesions were confined to myelin, while axons and nerve cells were spared, particularly in the early stages of the lesions. Numberous phagocytes were observed which contained engulfed myelin and other lipid breakdown products. Ultrastructurally and histochemically the disease differs from previusly described types of leucodystrophy.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00688706
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  • 72
    Digitale Medien
    Digitale Medien
    Sclérose tubereuse de bourneville chez un prématuré (1977)
    Springer
    Acta neuropathologica 40 (1977), S. 157-161 
    Details
    ISSN: 1432-0533
    Schlagwort(e): Tuberous sclerosis ; Electron microscopy ; Astrocytes ; Microvilli
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary A sporadic case of tuberous sclerosis in a stillborn infant is reported. The death at the 31st week of gestation was presumably due to the development of enormous rhabdomyomas of the heart. The typical cerebral lesions were fully developed as in patients deceased later in life. The atypical cells found in the cortical tubers demonstrated ultrastructural features of reactive astrocytes. Moreover, they showed innumerable microvillilike projections on their surface and junctional complexes, mostly of the zonula adhaerens type, reminiscent of ependymocytes. The significance of such glio-epithelial cellular features is discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00688705
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  • 73
    Digitale Medien
    Digitale Medien
    Electron microscopic studies on the cerebral lesions of rats in experimental chronic disulfiram poisoning (1977)
    Springer
    Acta neuropathologica 38 (1977), S. 221-224 
    Details
    ISSN: 1432-0533
    Schlagwort(e): Disulfiram psychosis ; Electron microscopy ; Synaptic changes ; Dopamine-β-hydroxylase ; Histotoxic anoxia
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary Following chronic administration of disulfiram to rats, changes of the brain were examined electron-microscopically. Pathological findings were observed in the nerve cells of the cerebral cortex and hypothalamus at later stage, and synaptic changes in the hypothalamus from initial stage. On the other hand, changes of myelinated fibers, neuroglias and capillaries were very slight. It was considered that neurons were affected more predominantly than other neuronal elements by the cytotoxic action of the drug, and that the synaptic changes of the hypothalamus might reveal chronic disturbance of noradrenergic transmission by inhibition of dopamine-β-hydroxylase. These ultrastructural findings might relate to the pathogenic mechanism of the disulfiram psychosis.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00688068
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  • 74
    Digitale Medien
    Digitale Medien
    Dense core vesicles around the Lewy body in incidental Parkinson's disease: an electron microscopic study (1977)
    Springer
    Acta neuropathologica 39 (1977), S. 173-175 
    Details
    ISSN: 1432-0533
    Schlagwort(e): Parkinson's disease ; Lewy body ; Dense core vesicles ; Electron microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary In a case of Lewy body disease incidentally found at autopsy, numerous dense core vesicles, 80–200 nm in diameter, were seen in the neuronal perikarya of the locus caeruleus. They were particularly numerous in the vicinity of the Lewy bodies. The change seems to occur at the early stage of Lewy body production and may represent an additional morphologic clue to abnormal catecholamine metabolism in Parkinson's disease.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00703325
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  • 75
    Digitale Medien
    Digitale Medien
    The incidence of ultrastructural abnormalities in the cortex of two retarded human brains (Down's syndrome) (1977)
    Springer
    Acta neuropathologica 37 (1977), S. 65-68 
    Details
    ISSN: 1432-0533
    Schlagwort(e): Electron microscopy ; Neuropathology ; Down's syndrome ; Human cerebral cortex
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary In a quantitative electronmicroscopic study, autopsy samples from the frontal and temporal lobes of two severely defective mongoloid brains were examined for the presence of abnormalities in the ultrastructure of the cerebral cortex. Particular attention was paid to the occurrence of atypical neuronal and glial inclusions similar to those which occur in small numbers (1 in 5000 µ2 of cortex) in neurologically normal brain (Rees, 1975). An area of 3.6×105 µ2 of cortex was examined from each brain. Within the cortical parenchyma, there was no gliosis, neuronal death or areas of degeneration. Atypical neuronal and glial inclusions were observed in both of the retarded brains, but they did not occur in substantially different numbers from normal brains. There were no inclusions or structural abnormalities peculiar to the retarded brains. Thus, in these two defective brains, it has not been possible to demonstrate any specific abnormalities in the ultrastructure of the cortex.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00684542
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  • 76
    Digitale Medien
    Digitale Medien
    Ultrastructural study of the vacuoles in the peripheral lymphocytes in juvenile amaurotic idiocy (1977)
    Springer
    Acta neuropathologica 38 (1977), S. 137-142 
    Details
    ISSN: 1432-0533
    Schlagwort(e): Lymphocyte ; Juvenile amaurotic idiocy ; Ceroid lipofuscinosis ; Vacuolar contents ; Electron microscopy ; Sural nerve ; Muscle ; Rectum
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary Lymphocytes of the peripheral blood of 31 patients with juvenile amaurotic idiocy (juvenile form of ceroid lipofuscinosis) were examined with the electron microscope. In all cases, intracytoplasmic clear vacuoles were present, containing round hollow, fingerprint and highly electron dense structures. The combination of these structures, not necessarily in one and the same vacuole, was considered to be highly indicative for the diagnosis of juvenile amaurotic idiocy. In addition to these three structures, parallel tubular inclusion bodies, rectilinear profiles and rodshaped structures were found but in a number of the cases. The parallel tubular inclusion bodies were not regarded as having any diagnostic significance.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00688560
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  • 77
    Digitale Medien
    Digitale Medien
    Infantile chronic peripheral neuropathy with giant axons (1977)
    Springer
    Acta neuropathologica 37 (1977), S. 213-217 
    Details
    ISSN: 1432-0533
    Schlagwort(e): Peripheral neuropathy ; Giant axons ; Electron microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary An 8-year-old boy with a slowly progressive motor neuropathy is described. The first signs appeared at the age of 3 years. Histological examination of the sural nerve showed the presence of numerous segmental axonal swellings and features of demyelination as well as remyelination. These enlargements were filled with irregularly orientated 10 nm filaments. The case resembled the previously described cases of giant axonal neuropathy but differed from them in absence of kinky hair.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00686881
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  • 78
    Digitale Medien
    Digitale Medien
    The ultrastructure of the extracellular phase of bone as observed in frozen thin sections (1977)
    Springer
    Calcified tissue international 23 (1977), S. 215-223 
    Details
    ISSN: 1432-0827
    Schlagwort(e): Amorphous mineral ; Bone ; Electron microscopy ; Ultracryotomy ; Ultramicro-incineration
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin , Physik
    Notizen: Summary The fine structure of the extracellular phase of avian medullary bone and embryonic chick femur was examined in thin sections prepared by ultracryotomy and ultramicroincineration. Since contact with solutions was completely avoided, little or no loss or dislocation of mineral constituents could occur. Amorphous bone mineral (ABM) was present in two forms: as 15–30 nm spheres and as a structure-free haze. Removal of all organic material by low temperature ashing left the ABM intact. Crystals were usually associated with the ABM. In newly ossifying regions clusters or nodules of randomly oriented crystals and ABM appeared to coalesce when they reached approximately 1 μm in diameter. In highly calcified regions crystals appeared to be oriented along collagen fibers. ABM did not appear to be associated with collagen. Unmineralized collagen was visible in osteoid after staining with dry OsO4 vapor and it appeared to be diverted around nodules. Structures which resembled matrix vesicles were present. Selected area electron diffraction patterns indicated the presence of hydroxyapatite.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02012788
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  • 79
    Digitale Medien
    Digitale Medien
    Ultrastructural study of amelogenesis imperfecta (1977)
    Springer
    Calcified tissue international 24 (1977), S. 191-197 
    Details
    ISSN: 1432-0827
    Schlagwort(e): Amelogenesis imperfecta ; Hypocalcification ; Hypoplasia ; Electron microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin , Physik
    Notizen: Summary An ultrastructural study of teeth with amelogenesis imperfecta revelaed various aspects of microcavities in the enamel surface, which ranged from isolated imprints of ameloblasts corresponding to the mildest lesions at the end of amelogenesis, to pits caused by the death of 20 to 30 ameloblasts at the beginning of amelogenesis. Abnormalities in the shape of the prisms can be observed. Further, crystals are distributed randomly within a prism or at the junction of 2 contiguous prisms while intercrystalline spaces are widened, indicating in various places the lack of a preferred orientation of the crystals. In amelogenesis imperfecta, two different crystalline periods are found: 1 of about 250 Å, the other of about 500 Å and over. The fact that amorphous areas are found among the crystals of enamel may be related to different stages of crystallization. However, it was not possible to find any lattice defect.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02223315
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  • 80
    Digitale Medien
    Digitale Medien
    Further observations on the fine structure of cellular cementum from deciduous teeth of pigs (1977)
    Springer
    Calcified tissue international 24 (1977), S. 239-242 
    Details
    ISSN: 1432-0827
    Schlagwort(e): Cementum ; Lysis ; Electron microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin , Physik
    Notizen: Summary Examination of microradiographs from the deciduous teeth of pigs revealed large lacunae or radiolucent zones close to the cemento-dentinal junction. Electron microscopic studies of the ground sections showed areas or irregularly shaped zones devoid of mineral and filled with collagen fibers. In the wide unmineralized zones, spherical clusters of crystallites were noted. Several cementum lacunae bordered by a broad rim of unmineralized collagen fibers were noted and some lacunae also contained zones of a moderately electron dense material. This material did not yield a diffraction pattern, while the mineralized part of the cementum gave the diffraction pattern typical of hydroxyapatite.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02223322
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  • 81
    Digitale Medien
    Digitale Medien
    Idiopathische ödeme, Kapillaropathie und eiweißreiche Körperhöhlenergüsse (1977)
    Springer
    Journal of molecular medicine 55 (1977), S. 1137-1147 
    Details
    ISSN: 1432-1440
    Schlagwort(e): Edema ; Capillary permeability ; Pericardial effusion ; Microcirculation ; Electron microscopy ; Ödem ; Kapillarpermeabilität ; Perikarderguß ; Mikrozirkulation ; Elektronenmikroskopie
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Beschreibung / Inhaltsverzeichnis: Zusammenfassung 2 Patientinnen hatten neben dem Krankheitsbild der idiopathischen Ödeme Höhlenergüsse insbesondere des Perikards. In dem einen Fall erforderte der große Perikarderguß schließlich die Perikardresektion, im anderen Fall führte Spironolacton-Dauerbehandlung zur weitgehenden Normalisieriung. Der relativ hohe Eiweißgehalt von Ödem (25 und 12 g/l) und Perikarderguß (55–61 und 48 g/l) und der vergrößerte Verteilungsraum für markiertes Eiweiß sprechen für vermehrte Permeabilität von kleinen Gefäßen und Grenzflächen für Eiweiß. Das Blutvolumen war niedrig normal bzw. erniedrigt. Elektronenoptisch fanden sich an den Hautgefäßen abnorme endotheliale Protuberanzen und große intercelluläre Lücken (Gaps) und an den Muskelgefäßen Befunde, die für einen gesteigerten cytopemptischen Transport sprechen. Die elektronenmikroskopischen Befunde der beiden Patientinnen zeigen keine prinzipiellen Unterschiede; sie stellen offenbar das morphologische Substrat gesteigerter Eiweißpermeabilität der Gefäße und der Bildung relativ eiweißreicher Ödeme dar. Damit sind morphologische Veränderungen bei Menschen mit idiopathischen Ödemen nachgewiesen, wie sie bisher nur in Tierversuchen mit experimentell erzeugten Ödemen beobachtet wurden.
    Notizen: Summary Clinical and electron microscopic studies of two female patients are reported who, in addition to the symptoms and signs of idiopathic edema, had pericardial (and pleural) effusions. In one patient pericardiectomy had to be performed, in the other patient pharmacotherapy with spironolactone was effective. In these patients the protein-concentrations of edema fluid (25 and 12 g/l) and pericardial fluid (55–61 and 48 g/l) were relatively high; the distribution space of labelled protein was increased; blood volumes were low-normal or decreased. Electronmicroscopy of the microvasculature showed identical alterations in both cases. In the cutaneous vessels the endothelium developed numerous abnormal cytoplasmic processes and intercellular “gaps”. In the capillaries of skeletal muscle our findings indicate an increase in vesicular transport. We suggest the electron microscopic alterations to be the morphologic correlate for the increased transport and extravascular accumulation of protein. Changes as they are described here in humans with idiopathic edema have been documented before in animals with experimental edema.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01478051
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  • 82
    Digitale Medien
    Digitale Medien
    Regeneration of cauda equina fibres after transsection and end-to-end suture (1977)
    Springer
    Journal of neurology 215 (1977), S. 81-90 
    Details
    ISSN: 1432-1459
    Schlagwort(e): Spinal nerve root ; Cauda equina ; Transsection and suture ; Regeneration ; Electron microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Beschreibung / Inhaltsverzeichnis: Zusammenfassung Die Regeneration in dorsalen und ventralen Nervenwurzeln der Cauda equina nach Durchtrennung und Naht am Schwein wurde nach dreimonatiger Überlebenszeit untersucht. Obwohl keine detaillierten quantitativen Analysen durchgeführt wurden, konnte eindeutig festgestellt werden, daß die Regeneration in motorischen Wurzeln ausgiebiger was als in sensiblen Wurzeln. In den regenerierten Wurzeln wurde eine Zunahme von Histiozyten, Fibroblasten und Collagen sowie eine Verdickung der Wurzelhüllen beobachtet. In regenerierten dorsalen Wurzeln fanden sich heterotope, vom Myelon ausgewachsene Gliabündel.
    Notizen: Summary The regeneration of nerve fibres in pigs was studied three months after transsection and end-to-end suture of the dorsal and ventral spinal nerve roots L 5 and S 1. Although no detailed quantitative analysis was performed, it was clearly evident that regeneration in motor roots is significantly more efficacious than in sensory roots. Probably as a reaction to Wallerian degeneration, an increase of histiocytes, fibroblasts and collagen fibrils, as well as a thickening of the sheaths was observed in the transsected and sutured roots. Heterotopic glial bundles were found in the regenerated dorsal roots.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00313055
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  • 83
    Digitale Medien
    Digitale Medien
    Regeneration of cauda equina fibres after transsection and end-to-end suture (1977)
    Springer
    Journal of neurology 215 (1977), S. 81-90 
    Details
    ISSN: 1432-1459
    Schlagwort(e): Spinal nerve root ; Cauda equina ; Transsection and suture ; Regeneration ; Electron microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Beschreibung / Inhaltsverzeichnis: Zusammenfassung Die Regeneration in dorsalen und ventralen Nervenwurzeln der Cauda equina nach Durchtrennung und Naht am Schwein wurde nach dreimonatiger Überlebenszeit untersucht. Obwohl keine detaillierten quantitativen Analysen durchgeführt wurden, konnte eindeutig festgestellt werden, daß die Regeneration in motorischen Wurzeln ausgiebiger was als in sensiblen Wurzeln. In den regenerierten Wurzeln wurde eine Zunahme von Histiozyten, Fibroblasten und Collagen sowie eine Verdickung der Wurzelhüllen beobachtet. In regenerierten dorsalen Wurzeln fanden sich heterotope, vom Myelon ausgewachsene Gliabündel.
    Notizen: Summary The regeneration of nerve fibres in pigs was studied three months after transsection and end-to-end suture of the dorsal and ventral spinal nerve roots L 5 and S 1. Although no detailed quantitative analysis was performed, it was clearly evident that regeneration in motor roots is significantly more efficacious than in sensory roots. Probably as a reaction to Wallerian degeneration, an increase of histiocytes, fibroblasts and collagen fibrils, as well as a thickening of the sheaths was observed in the transsected and sutured roots. Heterotopic glial bundles were found in the regenerated dorsal roots.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02402141
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  • 84
    Digitale Medien
    Digitale Medien
    Ultrastructural study of a muscle biopsy from a patient with subacute myelo-optic neuropathy (1977)
    Springer
    Journal of neurology 215 (1977), S. 91-102 
    Details
    ISSN: 1432-1459
    Schlagwort(e): SMON ; Muscle biopsy ; Electron microscopy ; Neuromuscular junction ; Paracrystalline inclusions
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Beschreibung / Inhaltsverzeichnis: Zusammenfassung Ein 46jähriger Mann, welcher über lange Zeit hohe Dosen von Clioquinol einnahm, entwickelte recht plötzlich charakteristische neurologische Ausfälle, entsprechend einer subakuten myelooptischen Neuropathie (SMON). Bei der elektronenoptischen Untersuchung einer Muskelbiopsie, die 5 Monate nach Beginn der Erkrankung entnommen worden war, zeigten sich ausgeprägte degenerative Veränderungen der präsynaptischen Nervenendigungen und vereinzelte parakristalline Einschlüsse in der Endplattenregion, die einmalig erscheinen. Letzteres könnte das morphologische Substrat der toxischen Substanz darstellen, die für den SMON verantwortlich gemacht wird.
    Notizen: Summary A man, aged 46, who had been taking Clioquinol in high doses for a long period, developed a characteristic neurological syndrome of subacute myelo-optic neuropathy rather abruptly. Electron microscopical examination of the muscle biopsy, obtained five months after the onset of the disease, revealed severe degenerative changes of the presynaptic nerve endings and some unique paracrystalline inclusions in the sole plate region. The latter may represent the morphological expression of the toxic agent which is held responsible for the subacute myelo-optic neuropathy.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02402142
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  • 85
    Digitale Medien
    Digitale Medien
    Ultrastructural study of a muscle biopsy from a patient with subacute myelo-optic neuropathy (1977)
    Springer
    Journal of neurology 215 (1977), S. 91-102 
    Details
    ISSN: 1432-1459
    Schlagwort(e): SMON ; Muscle biopsy ; Electron microscopy ; Neuromuscular junction ; Paracrystalline inclusions
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Beschreibung / Inhaltsverzeichnis: Zusammenfassung Ein 46jähriger Mann, welcher über lange Zeit hohe Dosen von Clioquinol einnahm, entwickelte recht plötzlich charakteristische neurologische Ausfälle, entsprechend einer subakuten myelooptischen Neuropathie (SMON). Bei der elektronenoptischen Untersuchung einer Muskelbiopsie, die 5 Monate nach Beginn der Erkrankung entnommen worden war, zeigten sich ausgeprägte degenerative Veränderungen der präsynaptischen Nervenendigungen und vereinzelte parakristalline Einschlüsse in der Endplattenregion, die einmalig erscheinen. Letzteres könnte das morphologische Substrat der toxischen Substanz darstellen, die für den SMON verantwortlich gemacht wird.
    Notizen: Summary A man, aged 46, who had been taking Clioquinol in high doses for a long period, developed a characteristic neurological syndrome of subacute myelo-optic neuropathy rather abruptly. Electron microscopical examination of the muscle biopsy, obtained five months after the onset of the disease, revealed severe degenerative changes of the presynaptic nerve endings and some unique paracrystalline inclusions in the sole plate region. The latter may represent the morphological expression of the toxic agent which is held responsible for the subacute myelo-optic neuropathy.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00313056
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  • 86
    Digitale Medien
    Digitale Medien
    Polyneuropathy in hypothyroidism (1977)
    Springer
    Journal of neurology 215 (1977), S. 103-114 
    Details
    ISSN: 1432-1459
    Schlagwort(e): Hypothyroidism ; Peripheral neuropathy ; Nerve biopsy ; Electron microscopy ; Renaut bodies
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Beschreibung / Inhaltsverzeichnis: Zusammenfassung Es werden die klinischen Daten und Nervenbiopsiebefunde von 4 hypothyreotischen Patienten, die Symptome einer diffusen peripheren Neuropathie aufwiesen, dargestellt. Klinisch-neurologisch bestanden sensiblen Reizzeichen von scherzhaftem Charakter, eine symmetrische, distale Sensibilitätsminderung mit Ataxie und eine Abschwächung der Muskeleigenreflexe mit Verlust der Achillessehnenreflexe. Bei einem Patienten war die periphere Neuropathie Leitsymptom der zugrunde liegenden metabolischen Erkrankung. Als Substrat dieser Ausfälle fand sich bei der licht- und elektronenmikroskopischen Untersuchung der N. suralis-Biopsien eine deutliche Verminderung der Markfasern, besonders die großkalibrigen, dickbemarkten Axone betreffend. Die Histogramme zeigten entsprechend ein pathologisches unimodales Faserspektrum. Dystrophische und degenerative Veränderungen an Axonen aller Faserklassen und das Vorkommen sogenannter „Regenerationsgruppen“ kleiner, dünnbemarkter Fasern deuten darauf hin, daß der Neuropathie bei diesen hypothyreotischen Patienten in pathogenetischer Hinsicht in erster Linie eine neuroaxonale Degeneration mit unvollständiger Regeneration zugrunde liegt.
    Notizen: Summary Clinical and sural nerve biopsy findings are described in four hypothyroid patients with manifestations of a diffuse peripheral neuropathy. Clinical examination revealed distal sensory inpairment, complaints of burning and lancinating extremity pains, ataxia and a decrease of deep tendon reflexes with total ankle jerk loss. In one patient, signs of the peripheral neuropathy comprised the leading symptomatology of the underlying metabolic disorder. Light and electron microscopic investigations of the sural nerve biopsies revealed a marked reduction of myelinated fibers, affecting mainly the large myelinated axons. The histograms showed a pathological unimodal spectrum of myelinated fiber diameters. Dystrophic and degenerative changes in axons of all fiber classes and the presence of clusters of thinly myelinated small fibers suggest that neuroaxonal degeneration and incomplete regeneration may represent the major pathogenetic mechanism of the neuropathy in these hypothyroid patients.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00313057
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  • 87
    Digitale Medien
    Digitale Medien
    Polyneuropathy in hypothyroidism (1977)
    Springer
    Journal of neurology 215 (1977), S. 103-114 
    Details
    ISSN: 1432-1459
    Schlagwort(e): Hypothyroidism ; Peripheral neuropathy ; Nerve biopsy ; Electron microscopy ; Renaut bodies
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Beschreibung / Inhaltsverzeichnis: Zusammenfassung Es werden die klinischen Daten und Nervenbiopsiebefunde von 4 hypothyreotischen Patienten, die Symptome einer diffusen peripheren Neuropathie aufwiesen, dargestellt. Klinisch-neurologisch bestanden sensiblen Reizzeichen von scherzhaftem Charakter, eine symmetrische, distale Sensibilitätsminderung mit Ataxie und eine Abschwächung der Muskeleigenreflexe mit Verlust der Achillessehnenreflexe. Bei einem Patienten war die periphere Neuropathie Leitsymptom der zugrunde liegenden metabolischen Erkrankung. Als Substrat dieser Ausfälle fand sich bei der licht- und elektronenmikroskopischen Untersuchung der N. suralis-Biopsien eine deutliche Verminderung der Markfasern, besonders die großkalibrigen, dickbemarkten Axone betreffend. Die Histogramme zeigten entsprechend ein pathologisches unimodales Faserspektrum. Dystrophische und degenerative Veränderungen an Axonen aller Faserklassen und das Vorkommen sogenannter „Regenerationsgruppen“ kleiner, dünnbemarkter Fasern deuten darauf hin, daß der Neuropathie bei diesen hypothyreotischen Patienten in pathogenetischer Hinsicht in erster Linie eine neuroaxonale Degeneration mit unvollständiger Regeneration zugrunde liegt.
    Notizen: Summary Clinical and sural nerve biopsy findings are described in four hypothyroid patients with manifestations of a diffuse peripheral neuropathy. Clinical examination revealed distal sensory inpairment, complaints of burning and lancinating extremity pains, ataxia and a decrease of deep tendon reflexes with total ankle jerk loss. In one patient, signs of the peripheral neuropathy comprised the leading symptomatology of the underlying metabolic disorder. Light and electron microscopic investigations of the sural nerve biopsies revealed a marked reduction of myelinated fibers, affecting mainly the large myelinated axons. The histograms showed a pathological unimodal spectrum of myelinated fiber diameters. Dystrophic and degenerative changes in axons of all fiber classes and the presence of clusters of thinly myelinated small fibers suggest that neuroaxonal degeneration and incomplete regeneration may represent the major pathogenetic mechanism of the neuropathy in these hypothyroid patients.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02402143
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  • 88
    Digitale Medien
    Digitale Medien
    Dorsal-ventral differentiation in Simonsiella and other aspects of its morphology and ultrastructure (1977)
    Springer
    Archives of microbiology 113 (1977), S. 197-204 
    Details
    ISSN: 1432-072X
    Schlagwort(e): Gliding bacterium ; Simonsiella ; Oral cavity ; Electron microscopy ; Morphology ; Dorsal-ventral differentiation ; Ultrastructure
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The morphology and ultrastructure of the aerobic, Gram-negative multicellular-filamentous bacteria of the genus Simonsiella were investigated by scanning and transmission electron microscopy. The flat, ribbon-shaped, multicellular filaments show dorsal-ventral differentiation with respect to their orientations to solid substrata. The dorsal surface, orientated away from the substrate, is convex and possesses an unstructured capsule. The ventral surface, on which the organisms adhere and glide, is concave and has an extracellular layer with fibrils extending at right angles from the cell wall. The cytoplasm in the ventral region contains a proliferation of intracytoplasmic membranes and few ribosomes in comparison to the cytoplasm in other parts of the cell. Centripetal cell wall formation is asymmetrical and commences preferentially in the ventral region. Quantitative differences in morphology and cytology exist among selected Simonsiella strains. Functional aspects of this dorsalventral differentiation are discussed with respect to the colonization and adherence of Simonsiella to mucosal squamous epithelial cells in its ecological habitat, the oral cavities of warm-blooded vertebrates.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00492025
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  • 89
    Digitale Medien
    Digitale Medien
    Electron microscopic investigation of the hydrogen-oxidizing acetate-forming anaerobic bacterium Acetobacterium woodii (1977)
    Springer
    Archives of microbiology 115 (1977), S. 207-213 
    Details
    ISSN: 1432-072X
    Schlagwort(e): Acetobacterium woodii ; Hydrogen-oxidizing acetate-forming anaerobe ; Fine structure ; Electron microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Acetobacterium woodii is a Gram-positive anaerobic nonsporeforming bacterium able to grow on H2 and CO2 as sole sources of energy. The product of fermentation is acetic acid. Fine structural analysis showed rod-shaped flagellated cells, and coccoid cells without flagella arranged predominantly in pairs and chains. The cell wall was found to be composed of three layers. The cell surface exhibited a periodic array of particles consisting of subunits. The cytoplasmic membrane showed particles either either in random distribution or in a hexagonal pattern. Intracytoplasmic membranes were rarely observed, whereas inclusion bodies of varying shapes, predominantly in an uncommon disc-shape, could frequently be observed. Their content was dissolved in ultrathin sections indicating hydrophobic nature.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00406376
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  • 90
    Digitale Medien
    Digitale Medien
    Isolation and partial characterization of normal and defective bacteriophages of gram-negative hydrogen bacteria (1977)
    Springer
    Archives of microbiology 115 (1977), S. 237-247 
    Details
    ISSN: 1432-072X
    Schlagwort(e): Defective lysogeny ; Alcaligenes eutrophus ; Simultaneous isolation technique ; Temperate bacteriophages ; Pseudomonas pseudoflava ; Biological characterization ; Electron microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Widespread defective lysogeny was detected in Alcaligenes eutrophus by electron microscopic analysis of cultures. Mitomycin C treatment of the cultures resulted in the production of defective (inco-) particles. Polysheaths were produced both with and without induction. With the simultaneous isolation technique six phages were isolated for hydrogen-oxidizing strains of the new species Pseudomonas pseudoflava. The phages were able to replicate under autotrophic conditions and were found to have a very restricted host range. Electron microscopic analysis allowed classification into two structural groups. Group I contained phages with contractile tails; group II contained phages with flexible, noncontractile tails. All but one (gb) of the new phages were shown to be temperate by isolation of lysogens and induction with mitomycin C.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00446448
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  • 91
    Digitale Medien
    Digitale Medien
    The mitochondrial complex in cryptophyceae (1977)
    Springer
    Archives of microbiology 112 (1977), S. 207-218 
    Details
    ISSN: 1432-072X
    Schlagwort(e): Cryptophyceae ; Algae ; Hemiselmis rufescens ; Chroomonas ; Cryptomonas ; Mitochondrial complex ; Cristae ; Electron microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The unitary nature of the mitochondrion and the characteristic flattened finger-like morphology of the cristae were demonstrated in the Cryptophyceae. Hemiselmis rufescens contained an unbranched vermi-form mitochondrion in contrast to the variously branched complex, comprising an interconnected peripheral and central reticulum, in Chroomonas sp. and strains of Cryptomonas. The systematic value of the shape and distribution of the mitochondria in the examined genera was suggested.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00429337
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  • 92
    Digitale Medien
    Digitale Medien
    Effect of carbon dioxide on pigment and membrane content in Synechococcus lividus (1977)
    Springer
    Archives of microbiology 115 (1977), S. 185-198 
    Details
    ISSN: 1432-072X
    Schlagwort(e): Synechococcus lividus ; Cyanobacteria ; Carbon dioxide ; Electron microscopy ; Bleaching-regreening
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The effect of carbon dioxide on pigment and membrane content in Synechococcus lividus was studied by depriving cells of CO2 and examining cell populations biochemically and by electron microscopy. After 120 h of CO2 deprivation, S. lividus lost all detectable chlorophyll a and C-phycocyanin. Such bleached cultures were “mustard yellow”, the result of approximately 1.8 times more carotenoid per cell than green control cultures. Although cells from beached cultures appeared morphologically identical to control green cells when examined by light microscopy, electron microscopic examination revealed them to be devoid of detectable thylakoid membrane. Thylakoid membrane could not be recovered by physical isolation or revealed by freeze etching of bleached S. lividus. In addition, inclusion bodies characteristically found in S. lividus were also absent. Reintroduction of CO2 into bleached cultures resulted in a rapid resynthesis of both chlorophyll a and C-phycocyanin. Electron microscopic examination of these regreening cultures revealed that thylakoid membrane was also rapidly resynthesized. Growth of regreened cultures did not occur until there was the synthesis of a full complement of chlorophyll a, C-phycocyanin, and thylakoid membrane. A time course study of the cytological events occurring during bleaching and regreening is presented.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00406374
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  • 93
    Digitale Medien
    Digitale Medien
    The importance of smooth muscle cells in the development of foam cells in the gastric mucosa (1977)
    Springer
    Virchows Archiv 372 (1977), S. 287-297 
    Details
    ISSN: 1432-2307
    Schlagwort(e): Stomach ; Lipid islands ; Smooth muscle cells ; Foam cells ; Electron microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary Foam cells in lipid islands of the stomach can develop from both histiocytes and smooth muscle cells. With increasing storage of lipid vacuoles in smooth muscle cells, loosening of the myofilament arrangement and decrease of the dense areas subjacent to the plasma membrane occurs. Endoplasmic reticulum and the cisternae of the Golgi-apparatus dilate, the cell organelles increase initially and the basement membrane of the smooth muscle cells is fragmentarily formed. Only in incompletely formed foam cells can the origin from smooth muscle cells be recognised, in their final state their histiogenesis is seldom apparent.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00432404
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  • 94
    Digitale Medien
    Digitale Medien
    Chronic megakaryocytic-granulocytic myelosis — An electron microscopic study including freeze-fracture (1977)
    Springer
    Virchows Archiv 375 (1977), S. 129-146 
    Details
    ISSN: 1432-2307
    Schlagwort(e): Myeloproliferative disorder ; Megakaryocytic-granulocytic myelosis ; Granulocyte lineage ; Bone marrow biopsy ; Freeze-fracture ; Electron microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary In 5 patients with chronic megakaryocytic-granulocytic myelosis (CMGM) bone marrow specimens were studied by electron microscopy to investigate possible abnormalities of the granulocytic cell lineage. Thin sections were compared with freeze-fracture replicas to elucidate further aspects of leucocyte cytology. The atypia exhibited in these cells (eosinophils, basophils and neutrophil granulocytes) consisted mostly of a disorganization of granulopoiesis with hyper- and hypogranulation, a pathological increase in the number of nuclear blebs and a maturation asynchrony sometimes leading to Pelger-like cell forms. Moreover, a presumptive stem cell was demonstrated in the erythopoietic and granulocytic cell lines resembling CFU cells. In conclusion, granulopoiesis in CMGM exhibited abnormalities as generally observed in chronic myelogenous leukaemia. When considered with our previous finding of malignancy in megakaryopoiesis, CMGM has to be classified as a myelosis of mixed cellularity.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00428101
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  • 95
    Digitale Medien
    Digitale Medien
    Enzymhistochemische, histometrische und ultrastrukturelle Untersuchungen von Milzen bei der Vinylchlorid-Krankheit (1977)
    Springer
    Virchows Archiv 375 (1977), S. 303-317 
    Details
    ISSN: 1432-2307
    Schlagwort(e): Vinylchloride-disease ; Splenomegaly ; Electron microscopy ; Enzyme histochemistry ; Morphometrics
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Beschreibung / Inhaltsverzeichnis: Zusammenfassung Durch histometrische, enzymhistochemische und elektronenmikroskopische Untersuchungen konnte nachgewiesen werden, da\ die pathologischen MilzverÄnderungen bei der Vinylchlorid-Krankheit nicht durch hepatisch bedingte Pfortaderdruckerhöhung verursacht werden, sondern auf einer Gewebsalteration durch das Vinylchlorid oder dessen Metabolite beruhen. Dabei sind vor allem die faserbildenden Zellen der Milz beteiligt. Faserassoziierte Reticulumzellen in der roten Pulpa und fibroblastische Reticulumzellen in der wei\en Pulpa werden zu übermÄ\iger Bindegewebsneubildung stimuliert. Hierdurch kommt es in der roten Pulpa zur bindegewebigen Verödung der FiltrationsrÄume mit einer Reduzierung der Pulpastrangmakrophagen und in der wei\en Pulpa zu einer Vernarbung der periarteriellen Lymphscheiden. Die Folgen dieser Fibrosierungsprozesse sind charakteristische VerÄnderungen in der quantitativen Zusammensetzung der Gewebskomponenten, die sich deutlich von denjenigen in Milzen bei Lebercirrhose und Milzvenenthrombose unterscheiden.
    Notizen: Summary By means of histometric, enzyme histochemical, and electron microscopic investigations it was demonstrated that the pathological changes in the spleen in vinylchloride-disease are primary. Fibroblastic cells are the only specific splenic cells involved. Fibre-associated reticulum cells of the red pulp and fibroblastic reticulum cells in white pulp are stimulated to produce excessive amounts of the extracellular elements of connective tissue, especially collagen fibrils. The newly formed connective tissue causes obliteration of extracellular blood channels in the red pulp and thus a reduction in the number of pulp-cord macrophages, and scarring of the periarterial lymphatic sheaths. The results of this fibrosing process are characteristic quantitative changes in the splenic histologic structures. These changes are different from those structural alterations occuring in spleens following extrasplenic hemodynamic changes, such as thrombosis of the splenic veins or cirrhosis of the liver.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00427061
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  • 96
    Digitale Medien
    Digitale Medien
    Light-electron microscopic and cytochemical studies on the morphogenesis of familial medullary thyroid carcinoma (1977)
    Springer
    Virchows Archiv 376 (1977), S. 29-46 
    Details
    ISSN: 1432-2307
    Schlagwort(e): Thyroid gland ; Familial medullary carcinoma ; Morphogenesis ; Electron microscopy ; Cytochemistry of C-cells
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary Six cases of familial medullary thyroid carcinoma (MTC) were investigated by light and electron microscopy as well as by ultracytochemical methods. Light microscopic examination revealed multifocal C-cell proliferation in 5 subjects. These cells were mostly limited to thyroid follicles, but occasionally extended across the follicular capsule forming microscopic MTC. Electron microscopic examination showed that, in some follicles, the proliferating C-cells were still covered by a continuous layer of follicular cells, whereas in others the proliferation extended to the follicular center. C-cells were in direct contact with the colloid, and ultramicroinvasion of the follicular capsule was detected. These observations are consistent with the hypothesis that familial MTC seems to begin as multifocal C-cell proliferation, limited at first to thyroid follicles, between the capsule and the follicular epithelium. Later, the proliferation extends to the follicular center, and C-cells come in contact with the colloid, at which time an in situ carcinoma stage is reached. Some neoplastic cells invade the follicular capsule and, finally, multiple MTC appear and eventually conglomerate. Generally, there were no constant morphologic criteria for a dysplasia or neoplasia among the proliferating C-cells limited to thyroid follicles, when compared with normal or even malignant C-cells. For these reasons, a hyperplastic or dysplastic process preceding MTC cannot be clearly distinguished from a neoplastic process. Our study, however, shows that a light microscopic, apparently hyperplastic process may be a malignant one. Amyloid was present in the more voluminous MTC, associated with tumor cell necrosis, but it was not evident in small MTC and within the foci of C-cell proliferation. Ultracytochemical techniques revealed that the secretory granules of normal, proliferating and neoplastic C-cells contained polysaccharides and/or glycoproteins.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00433083
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  • 97
    Digitale Medien
    Digitale Medien
    Electron microscopic study of signet-ring cells in diffuse carcinoma of the human stomach (1977)
    Springer
    Virchows Archiv 374 (1977), S. 275-284 
    Details
    ISSN: 1432-2307
    Schlagwort(e): Signet-ring cell ; Scirrhous carcinoma ; Gastric carcinoma ; Electron microscopy ; Stomach cancer
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary The signet-ring cells seen in gastric carcinoma of the diffuse type were studied by light and electron microscopy. A classification of these cells into Types A, B and C was developed, based on intracellular mucous granules, nuclei and organelles, and by reactions to PAS and Alcian-blue staining. The gradual transition of Type A cells to Type B and the subsequent change of Type B to Type C suggest successive stages in maturation. These cells, especially those of Type B, resemble the mucous neck cells of the corpus and the glands of the pylorus.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00432651
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  • 98
    Digitale Medien
    Digitale Medien
    En face organ cultures of rabbit aortic segments after a single dilatation trauma in vivo (1977)
    Springer
    Virchows Archiv 375 (1977), S. 257-262 
    Details
    ISSN: 1432-2307
    Schlagwort(e): Rabbit aorta ; Organ culture ; Endothelial regeneration ; Electron microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary Following an in vivo lesion of the rabbit thoracic aorta, endothelial regeneration was studied in silver-stained specimens from en face organ cultures of aortic segments with and without intercostal arteries. The in vivo lesion had been inflicted by an embolectomy catheter. Our results confirmed that endothelium regenerates from preexisting endothelium around the mounths of the intercostal arteries. A conspicuous observation was the orientation pattern of endothelial cells during regeneration. Transmission electron microscopy suggests that the cell cytoskeleton plays a role in the regulation of regeneration. The organ culture technique described, complementary to in vivo and in vitro investigations with cell cultures, seems to be a valuable tool in further investigations on factors involved in the repair of arterial tissue.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00427057
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  • 99
    Digitale Medien
    Digitale Medien
    Elektronenmikroskopische Befunde am Herzmuskel der Ratte bei experimentell induzierten ElektrolytverÄnderungen (1977)
    Springer
    Virchows Archiv 375 (1977), S. 331-344 
    Details
    ISSN: 1432-2307
    Schlagwort(e): Catecholamine ; Angiotensin ; Ouabain ; Dihydrotachysterol ; Rat heart ; Myocardial necrosis ; Electrolyte concentration ; Electron microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Beschreibung / Inhaltsverzeichnis: Zusammenfassung Noradrenalin und Angiotensin in hohen Dosen allein oder in Kombination mit Strophanthin appliziert, führen bei der Ratte zu einer Erniedrigung des Gesamtcalciumgehaltes des Myocard. Dihydrotachysterol (DHT) erhöht den Calciumgehalt und zeigt elektronenmikroskopisch SchÄdigungen an Mitochondrien und Myofibrillen. Die kombinierte Gabe von DHT und Noradrenalin bzw. Angiotensin potenziert den Calciumwert, erhöht das Magnesium und lÄ\t Kalium nahezu unverÄndert. Die im Bereich der linksventrikulÄren Innenschichten ausgebildeten „Catecholaminnekrosen“ finden sich nach DHT-PrÄmedikation ubiquitÄr. Strophanthin verhindert den massiven, durch DHT- und Angiotensinmedikation induzierten Calciumstieg, wÄhrend es bei der kombinierten Gabe von DHT und Noradrenalin keine Wirkung entfaltet. Myofibrillen und Mitochondrien sind verÄndert, Calciumablagerungen sind nachweisbar, charakteristische Unterschiede fehlen.
    Notizen: Summary Changes in certain tissue electrolytes brought about by injecting rats with noradrenalin (NA), angiotensin or dihydrotachysterol (DHT) were compared with the concomitant ultrastructural alterations of cardiac muscle. High doses of NA or angiotensin given singly or together with ouabain were found to lower the overall myocardial calcium concentration without accompanying structural lesions. When calcium was increased more than tenfold by the administration of DHT, electronmicroscopy revealed altered myofibrils and mitochondria. Giving DHT along with NA or angiotensin not only preserved their calcium-lowering effect, but resulted in a massive increase in tissue calcium, with magnesium levels also rising, whilst the tissue content of potassium remained almost unchanged. Following NA administration the necrotic areas, known to be confined to the inner endocardial layer after catecholamine administration, pervaded the entire cardiac muscle mass. Ouabain prevented the massive rises of tissue calcium induced by DHT given together with angiotensin but was ineffective when added to the combination of DHT and NA. In either case, both myofibrils and mitochondria displayed ultrastructural alterations although distinctive features were lacking. The data suggests that DHT acts on cellular calcium, not only by mobilising calcium from osseous structures but also by interacting with intracellular calcium homeostasis. In addition to overstimulating contractile elements, calcium is thought to exert its noxious effect in a more generall way, e.g. by binding to surface membranes, thus affecting the normal functioning and eventually the structure of mitochondria and other organelles besides myofibrils.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00427063
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  • 100
    Digitale Medien
    Digitale Medien
    Degenerative processes in the pathogenesis of pulmonary alveolar lipoproteinosis (1977)
    Springer
    Virchows Archiv 376 (1977), S. 165-174 
    Details
    ISSN: 1432-2307
    Schlagwort(e): Lung ; Electron microscopy ; Pulmonary alveolar proteinosis ; Degeneration
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary Electron microscopy in an infant of 4 months with pulmonary alveolar lipoproteinosis showed filling of the alveoli with osmiophilic lamellar bodies. Similar structures were present in the cytoplasm of type I alveolar epithelial cells and to a lesser extent in the capillary endothelium and interstitium. These changes represent widespread degenerative processes in the lung caused by an unidentified cytotoxic agent. In this patient the disease is comparable to the drug-induced cytotoxic animal model and differs from the dust-induced hypersecretory animal model.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00432587
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