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  • 1965-1969  (1,903)
  • Industrial Chemistry  (1,519)
  • Biochemistry and Biotechnology  (352)
  • Engineering General
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  • 1
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969), S. 207-237 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Growth kinetics of heterogeneous populations of sewage origin were studied in completely mixed reactors of the once-through type at a high concentration of incoming substrate, 3000 mg/l glucose, and in systems employing cell feedback or sludge recycle at an incoming substrate concentration of 1000 mg/1 glucose. The recycle flow rate employed was 25% of the incoming feed flow, and the concentration of cells in the recycle was maintained as closely as possible at 150% of the cell concentration in the reactor. Studies were made at various dilution rates. Throughout these studies, batch experiments using cells grown at the various dilution rates were made to determine ks and μm values. As in previous studios using heterogeneous populations, the relationship between specific growth rates μ and substrate concentration S was represented better by the Monod equation than by any other which was tested. The growth “constants” μm, ks, and Y were found to fall in the same general range as those determined in previous studies in once-through systems operated at 1000 mg/l glucose. It was observed that cell recycle, even at the relatively low concentration factor employed in these studies, greatly enhanced the flocculating and settling characteristics of the cells.
    Additional Material: 9 Ill.
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  • 2
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969), S. 263-266 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 2 Ill.
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  • 3
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969) 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969), S. 283-292 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A pilot-scale process for the isolation of an aliphatic, amidase from Pseudomonas aeruginosa has been developed. A constitutive, partially irrepressible mutant was employed to give a high initial enzyme concentration. An existing laboratory isolation procedure has been scaled up and modified particularly by substitution of polyethylene glycol for ammonium sulfate precipitation as the first stage in the conversion of the fractionation to continuous operation. Full recovery of activity was achieved with the modification. The recovery of enzyme from a subsequent chromatographic stage was 85% and the maximum overall purification was 28-fold.
    Additional Material: 3 Ill.
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  • 5
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969), S. 293-321 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Kinetic studies on fermentation processes were made and a general equation of production rate was newly presented applying the kinetic theory on mierobial cell growth which was reported previously by the authors.l,2 Equations for product concentration in fermentation time courses were derived by developing mathematically the general equation of production rate, and characteristic properties of fermentation processes were clarified. Some examples of fermentations were analyzed kinetically using the new kinetic theory. The calculated values of product, and cell concentrations were in good agreement with the observed values.
    Additional Material: 22 Ill.
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  • 6
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A procedure for measuring the rate of heat production from a fermentation has been developed. The method is based on measuring the rate of temperature rise of the fermentation broth resulting from metabolism, when the temperature controller is turned off. The heat accumulation measured in this manner is then corrected for heat losses and gains. A sensitive thermistor is used to follow the temperature rise with time. This procedure is shown to be as accurate as previous methods but much simpler in execution. Using this technique, the rate of heat production during metabolism was found to correlate with the rate of oxygen consumption. Experiments were performed using bacteria (E. coli and B. subtilis), a yeast (C. intermedia), and a mold (A. niger). The substrates investigated included glucose, molasses, and soy bean meal. The proportionality constant for the correlation is independent of the growth rate, slightly dependent on the substrate, and possibly dependent On the type of organism growth. This correlation has considerable potential for predicting heat evolution from the metabolism of microorganisms on simple or complex substrates and providing quantitative parameters necessary for heat removal calculations.
    Additional Material: 5 Ill.
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  • 7
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969), S. 323-335 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A steam sterilizable oxygen electrode for fermentor use is described. The electrode has a silver cathode, lead anode, phosphate electrolyte, and a membrane of a fluorinated ethylene-propylene copolymer film (FEP.).The electrode has a linear response to partial pressure of oxygen from 1.5 × 10-2 to 103 mm Hg.
    Additional Material: 8 Ill.
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  • 8
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969), S. 337-348 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Penicillin amidase was extracted from Escherichia coli ATCC 9637, grown on phenylacetic, acid and glutamate, and purified by fractional ion with streptomycin sulphate, ammonium sulphate and polyethylene glycol, followed by chromatography on DEAE-cellulose. The purification factor was 100-200 × and the overall yield was about 115%. The enzyme was chemically attached to derivatives of cellulose and the kinetics of these insolubilized penicillin amidase preparations was investigated.
    Additional Material: 4 Ill.
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  • 9
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969), S. 363-380 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The properties of β-galactosidase attached to cellulose and DEAE-cellulose sheets arc described. Those insoluble enzyme derivatives obey the Michael-Menten relationship but, the measured kinetic parameters are very dependent on the flow conditions. The results of long-term stability tests are given.
    Additional Material: 11 Ill.
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  • 10
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969), S. 349-362 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Amyloglucosidase (EC. 3.2.1.3), partially purified from an Aspergillus species, was chemically attached to DEAE cellulose using the bifunctional reagent 2-amino-4,6-dichloro-s-triazine. The action of the insolubilized enzyme derivative on dilute maltose and dextrin solutions was studied in a packed bed. A second and deeper bed was used to demonstrate the possibility of a continuous process for raising the dextrose; equivalents of “glucose” liquors of high concentration formed by acid hydrolysis of maize starch.
    Additional Material: 8 Ill.
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  • 11
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
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  • 12
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969), S. 383-391 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The results achieved by the cultivation of the yeast. Candida lipolytica on gas oil are referred. By using a distillation fraction of gas oil distilling between 180-400°C, containing 10-20% of n-alkanes, the optimal condition for biomass production and deparaffination were estimated for various dilution rates and various amounts of gas oil in the medium. The main factor, which influences the yield coefficient by hydrocarbon fermentation is the polyauxie of the hydrocarbon substrate. The penetration of dispersed hydrocarbons into the yeast cell is demonstrated on electron micrographs and the velocity and reversibility of this process is estimated by using tritium-traced hexadecane.
    Additional Material: 6 Ill.
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  • 13
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969), S. 409-416 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A Micrococcus cerificans strain was grown on simple media with n-hexadecane or gas oil as sole carbon sources. Samples of cellular material recovered from hexadecane or gas oil fermentations do not appear to differ significantly in their composition. The protein content varied from 68 to 75%. With the exception of sulfur amino acids the amino acid distribution compares favorably with the FAO standard reference protein.The biological value of cell protein recoveered from hexadecane fermentations was 67 (cascin, 70). In the case of gas oil grown cells, the cell material recovered had to be completely purified in order to improve its protein quality. After fully extraction of undersirable fraction with petroleum ether in a Soxhlet apparatus the biological value observed was 63.
    Additional Material: 1 Ill.
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  • 14
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969), S. 843-851 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Continuous phased growth produces a culture in which most of the cells in the population are in the same stage of their development. The cell, thereby amplified by the size of the synchronous population, may be examined in the phased culture at any desired growth rate. Changes taking place in the cell after the cell cycle, i.e., post-cycle changes, may be examined by a modification of the procedure. Further systematic applications of the method permit a rational approach to problems of cell growth and metabolism.The phasing technique recognizes the cells as the fundamental unit for experimental investigation, and offers a great potential in the analysis of the cell throughout its cycle, a relatively unexploited field in cell physiology and fermentation. Experiments with yeasts and bacteria illustrate some of the applications and progress made so far.
    Additional Material: 7 Ill.
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  • 15
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969), S. 785-804 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The physiology of Aspergillus nidulans strain 224 has been studied under conditions of batch- and glucose-limited chemostat-culture and the effect of different steady state growth rates and dissolved oxygen tensions (DOT) examined. Measurements of the specific activities of selected glucose enzymes, the extent of oxygen uptake inhibition by glycolytic inhibitors, and radiorespirometric analyses were made in order to follow the variations in glucose catabolism, which occurred under these conditions. Greatly increased activity of the hexosemonophosphate (HMP) pathway was found during: (i) exponential growth of batch cultures; (ii) at near maximum specific growth rates (μ = 0.072 hr-1) (DOT = 156 mm Hg); and (iii) at low DOT levels (〈30 mm Hg) (μ = 0.050 hr-1) in chemostat cultures. These changes in glucose eatabolism have been discussed in terms of the biosynthetic demands of the fungus under the influence of changing growth pressures. Preliminary studies also have been made of transition state behavior following stepwise alteration of the DOT. A new steady state was established after 4-5 culture doublings during which period an “overshoot” in HMP pathway activity occurred; these kinetics are indicative of a derepression of certain glucose enzymes. Low molecular weight phenols are synthesized during the exponential phase in batch cultures and these are further metabliized to a major secondary metabolite, melanin, at the onset of stationary phase conditions. The kinetics of tyrosinase production in steady state chemostats differs from those that might be predicted for an enzyme associated solely with secondary metabolism. A primary physiological role for this oxidase in Aspergillus nidulans has been postulated.
    Additional Material: 11 Ill.
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  • 16
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969), S. 853-862 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Continuous culture in a cascade of vessels with the addition of supplemental nutrients to any stage permits adjustment of the physiological state of the culture in each stage to best achieve a desired performance goal. The yeast Saccharomyces cerevisiae in two-stage continuous cultivation was selected as a model system. With conditions in the first stage held constant- at a selected glucose concentration in the feed stream, dilution rate for the second stage was varied. Cell numbers, dry weight, glucose concentration, respiration coefficient, and titers of several enzymes were determined. The seed rate was defined as the ratio of glucose concentration in the feeds to stage 1 and to stage 2. At low seed rates, the calculated specific growth rate in the second stage was proportional to dilution rate. At higher seed rates, the specific growth rate based on dry weight behaved differently from that based on cell numbers, and the dependence on dilution rate was not linear.
    Additional Material: 5 Ill.
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  • 17
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969), S. 887-907 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The primary objective of this paper was to develop a mathematical description for the food chain, Because of the interdependence of the elements in this food chain, continuous oscillations among the variables are possible. A set of three differential equations was obtained to describe the above system in a continuously fed stirred tank reactor. The differential equations obtained were examined to characterize the possible types of solutions. A limit, cycle solution was obtained for some values of the system parameters.
    Additional Material: 9 Ill.
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  • 18
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969), S. 863-874 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A high intensity light system (HILIS) was designed and constructed to define the environmental parameters affecting production of algae. The HILIS incorporates the basic concepts of an aerobic fermenter for heterotrophic cells with high intensity illumination for photosynthetic studies. Of nine parameters considered, temperature and light intensity studies using Chlorella 71105 have been completed. Total illumination was varied from 25,000 to 300,000 lumens (30 times intensity of sunlight as measured at earth's surface) in 7.7-1, culture. The effect of illumination upon growth was measured as cell concentration and total daily algal production when operating the HILIS as a continuous system at a dilution rate of 0.91 per day.Growth may be expressed as a long function of illumination. A maximum algal concentration of 25.5g/l., dry weight basis, was attained at 300,000 lumens.
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  • 19
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969), S. 875-885 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Homogeneous technique facilitates the cultivation of large quantities of cells, reduces the risk of contamination by eliminating many manipulations, and makes practical the control of conditions such as pH and oxygen tension. Although most animal cells will not multiply in free suspension, certain cell lines have lost the requirement of being attached to a solid surface. These cells can be subcultured indefinitely but have some resemblance to cancer cells such as their abnormal karyotype. Certain cell linen developed from human embryonic tissue maintain their diploid character after repeated subculture and would seem to be ideal for the production of vaccines. However, strict regulations exist for viral products for human injection in that only cells taken from normal tissue and subcultured but once may be used.A microcarrier method in which cells adhere to DEAE-Sephadex beads permits a suspension culture which may be termed quasihomogeneous. The attached cells may be retained by sedimentation or by screening as the medium is replaced. Cell debirs from the original tissue is difficult to remove from microcarrier cultures; modifications of the trypsinization technique have alleviated but not solved this problem.Conditions for virus replication can be less critical than those for cell growth in that oxygen tension seems to have little influence on virus production. In cases where rate of virus production increases with specific growth rate of cells, homogeneous culture would have a advantage in maintaining a high cell mogeneous culture would have a valuble advantage in maintaining a high cell growth rate for a longer time. Some virus infections destroy cells, but others cause little change in cellular mteabolism except that virus is continually produced. The latter type can be conducted with a microcarrier in continuous culture with a virus titer exceeding 107 plaque forming units per milliliter for over 50 days with Rubella-infected BHK cells.
    Additional Material: 7 Ill.
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  • 20
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969), S. 909-909 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
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  • 21
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969), S. 911-926 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Microorganisms were continuously cultivated in multistage column consisting of ten perforated plate sections to which medium and air were supplied concurrently from the bottom.At steady state the cell concentration in the various stages was gradationally differentiated from the bottom to the top in the direction of medium flow. RNA content per unit cell concentration at each sage was determined. The cells in the lower stages were higher in RNA content than those from the upper stages. Wash out was observed to occur in the column at dilution rates which do not result in wash out in a single stage chemostat system.A study of the flow characteristics revealed that the overall performance of the plate column was equivalent to that of a multistage system, when hole diameter and hole area to column cross sectional area ratio were properly selected. This was true even in highly aerated conditions. These results indicated that the perforated plates in the column hindred intermixing through the plates, and that each stage functioned as an independent stirred vessel. Industrial and research application of this type fermentor was discussed.
    Additional Material: 12 Ill.
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  • 22
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969), S. 927-943 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The design of a continuous column fermentor with a multiple staging effect is described. The column is divided into four compartments by horizontal perforated plates and is provided with a central agitator shaft driving an impeller in each compartment. A tube at the center of each plate forms a liquid seal around the shaft and also acts as a “downcomer.”The fermentor is normally operated with counter-current flow of gas and medium. Fresh medium is added to the top stage and product is withdrawn from the bottom.The effect of plate and agitator design on fermentor performance was studied in terms of factor such as oxygen transfer rate, gas holdup, and interstage mixing. By proper choice of the design parameters, the fermentor was made to approximate a perfect four-stage cascade in terms of reactor performance.Preliminary experiments were performed with air-water systems, but a more realistic picture of fermentor performance was obtained in experience involving propagation of Escherichia coli. Data for business and substrate concentrations in each stage confirmed the staging effect of the apparatus. The fermentor operated in a stable manner for periods of more than two weeks.
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  • 23
    Electronic Resource
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    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969), S. 967-985 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A description is given of the design and operation of high-power magnetic drives developed to enable shaft seals and glands to be dispensed within deepculture vessels, in tissue homogenizers, and in mixing and filling processes where sterility is essential. The drives operate at speeds of 300 to 2000 rpm in volumes of 300 1. to 10 ml with clearances up to 16 mm between the pole faces of the magnets.Two types of drive are described, one in which the driving and driven magnets form an integral unit on the lid of a vessel: such vessels are used for transporting material. To intiate stirring, it is only necessary to connect a motor directly, or through a cable-drive, to the magnetic-drive assembly. In the other type of unit the driving magnet is attached permanently to the driving motor. Locating pins on the base of the motor and corresponding sockets on the lid of the vessel ensure that when the motor is in position, the driving and driven magnets are located correctly in relation to one another.The design of these drives is based on the use of multipole, ceramic magnets. The advantages of their use in such units, compared with metal magnets, are discussed. Earlier magnetic drives are also discussed and explanations offered for the difficulties formerly met in scaling up.
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  • 24
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    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969), S. 945-966 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A multistage tower laboratory fermentor has been constructed consisting of eight compartments separated by sieve plates. Flow of substrate and air is concurrent from the bottom to the top of the column. It, was hoped that this system could be used to reproduce, simultaneously on a continuous basis, eight distinct phases of a batch growth curve. It was believed that the extent of batch curve simulation would depend upon the character of hydraulic mean residence time of broth in the column and in the individual compartments. The expected relationship did not occur. Rather it was found that growth in the column involved residence time characteristics not only for the fluid but also for the microorganisms, and for the growth limiting substrate. Depending upon the column operation, these could be distinct and different.The purpose of this investigation was to study the residence time distribution (RTD) of the continous (fluid) and dispersed (microorganisms) phases for model systems as well as for a yeast fermentation. Various degrees of flow nonideality, i.e., fluid blackflow and dispersed phase sedimentation, were noticed. The former seems to be due to interaction of the concurrent gas and liquid flow; it is particularly dependent upon void area of the sieve plate holes. Sedimentation is probably a function of plate design as well as cell size and density. It wa concluded that for a particular plate design the gas hold-up wass controlled by superficial air velocity and was the main parameter governing the differences between dispersed and continous phase(Rt1). This conclusion was supported by a computeraided styudy utilizing a mathematical model of fluid flow to fit the growth kinetics and cell distribution observed experimentally throughout the fermentor.Some advantages of foam control in the tower fermentor by surface active compounds are mentioned. Also, suggestions are made for carrying out fermentations that have two liquid phases, such as a hydrocarbon fermentation. The possibility of closely approximating plug-flow conditions in the multistage tower fermentor, a necessary condition for batch growth simulation, is discussed from a practical point of view.
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  • 25
    Electronic Resource
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    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969), S. 1005-1010 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A flow cell photometer is described with automatic cleaning of the photometric cell, denasimetric separation of air bubbles and precipitates, and a constant sensitivity from 0 to 10 mg/ml of bacterial dry weight.
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  • 26
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Experiments were performed on a cellulose acetate ultrafiltration membrane (HF-200, ABCOR Inc., Cambridge, Mass.) to test its efficacy in concentrating and purifying a crude enzyme (trypsin) preparation. Studies were also made to determine the influence of inorganic salts, pressure, and temperature on the rate of ultrafiltration for this membrane. The results showed reductions in the rates will be encountered due to the presence of inorganic salts. However, the reduced rates were still sufficiently high to make this method extremely attractive. Operating at filtration pressures above 75 psi at, 20 to 30°C for this membrane does not show any beneficial effect in terms of ultrafiltration rates. However, at 10°C there were continual increases in the filtration rates up to 100 psi. Concentration and purification studies with trypsin yielded a concentration factor of 8.35 and a purification factor 2.35. It was shown concretely that the purification of the enzyme was due to the passage of low molecular weight proteins (below 20,000) through the membrane. Enzyme activity slightly greater than 90% was obtained: 70% was found in the concentrate and 20% in the filtrate. It is concluded that membrane ultrafiltration is an ideal simple, rapid, and economical method for the recovery of biological active substances.
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  • 27
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    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969), S. 1027-1032 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 4 Ill.
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  • 28
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    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969), S. 1011-1025 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A number of improvements have been made in a totally-automated antibiotic bioassay machine previously described. The new machine accepts unmeasured, untreated, opaque suspensions of fermentation beers three times faster (120 samples per hour) and supplies printed potencies sooner (in just over two hours). Whereas the original machine employed a self-cleaning filter and used disposable two milliliter beakers, this version involves a batch-dialysis scheme for effecting sample purification, and provides for automated cleaning of incubation chambers.In operation, a measured, portion of thoroughly-mixed fermentation beer is automatically diluted and transferred into one side of an incubation chamber, the two halves of which are separated by a dialysis membrane. The other half is filled with inoculated media. During the two hour incubation at 37°, dialyzable antibiotic limits growth of the inoculum in proportion to its concentration. After incubation, the turbidity of the inoculum is simultaneously read by an online computer and plotted on a strip chart recorded. The computer suplies printed potency values and sample identification on site, while the recording provides the operator with an analog record of turbidity. Fiber optics are employed in the turbidmetric readout, and an electric typewrite provides the printout.
    Additional Material: 18 Ill.
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  • 29
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 3 Ill.
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  • 30
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    Biotechnology and Bioengineering 11 (1969), S. 1037-1041 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 1 Tab.
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  • 31
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    Biotechnology and Bioengineering 11 (1969) 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
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  • 32
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    Biotechnology and Bioengineering 11 (1969), S. 1043-1054 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A mixed culture of methanol oxidizing bacteria has been cultivated on simple inorganic salts medium supplemented with methanol. Optimal growth occurred at 31°C, pH 6.0-6.3, and a methanol concentration between 1 and 2 ml/1, of medium. The maximum yield was 4.5 g dw/I and the mean generation time 3.2 hr.It was estimated that 41% of methanol carbon was converted into cell-carbon, and that 73% of the inorganic nitrogen was converted to organic nitrogen.
    Additional Material: 9 Ill.
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  • 33
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    Biotechnology and Bioengineering 11 (1969), S. 417-426 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Utilization of n-heptane by a Pseudomonad was studied in pilot-size butch cultures. Optimal pH and temperature were determined by a factorial design and a medium based upon mineral uptake rates was formulated. High cell yields were obtained by volatilizing heptane in the incoming air and thereby achieving good hydrocarbon dispersion. Hydrocarbon carried by effluent gases was recovered and recycled. In cultures where pH is not controlled, decrease in the electrolytic conductivity of the medium was found to be indicative of viable cells and was used in monitoring bacterial propagation. If not checked, increase in salinity in pH controlled cultures was found to affect cell production negatively. Viscosity changes were not very significant. Heptane to aqueous medium ratio was found to affect oxygen supply to the system due to higher dissolved oxygen concentrations associated with hydrocarbons.
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  • 34
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    Biotechnology and Bioengineering 11 (1969), S. 719-724 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 5 Ill.
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  • 35
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    Biotechnology and Bioengineering 11 (1969) 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
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  • 36
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    Biotechnology and Bioengineering 11 (1969), S. 725-730 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 1 Ill.
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  • 37
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    Biotechnology and Bioengineering 11 (1969), S. 731-743 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A fairly general model of the biochemical oxidation, which takes into account the activity of microorganisms, is presented. Parameters of the model have been determined by fitting the model to available experimental data through the use of a straightforward gradient technique.
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  • 38
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    Biotechnology and Bioengineering 11 (1969), S. 745-756 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A temperature-synchronized, semi-continuous culture and monitoring system is described, with results from use of the system for autotrophically growing Euglena. Outflow from the culture vessel consists of measured samples taken automatically at 2-hour intervals and fixed for later counting. Inflow is by siphon feed, which restores the culture level after each sampling. The interpretation of growth curves obtained from such cultures is discussed from the viewpoint, of division synchrony and cell cycle studies, and some general comparisons are made between batch and continuous cultures for such studies.
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  • 39
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    Biotechnology and Bioengineering 11 (1969), S. 1111-1123 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Streplomyces griscus var. X-2455 produces an antibiotic complex which is active in vitro against a number of gram-positive and gram-negative bacteria and in mice against systemic infections caused by K, pneumoniae and D, pneumoniac. In view of the favorable chemotherapeutic index and the broad in vitro spectrum of crude concentrates, isolation of the pure antibiotic complex and the individual constituents was undertaken. The antibiotics referred to as Ho 5-2667, Ro 7-7730, and Ho 7-7731 can be differentiated by tle, ultraviolet light absorption spectra, and in vitro antibacterial activities. They all contain iron and may be classified as sideromycins.From antibiotic concentrates an antibacterially inactive substance was isolated and identified as N-acetyltyramine.
    Additional Material: 3 Ill.
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  • 40
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    Biotechnology and Bioengineering 11 (1969), S. 1125-1134 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The current knowledge concerning the biosynthesis of chloramphenicol is discussed. Cultures of Streptomyces sp. 3022a fed 14C-shikimie acid incorporated the label to the same extent into phenylalanine, tyrosine, and chloramphenicol. Of possible precursors of the phenylpropanoid nucleus of this antibiotic only p-aminophenylalanine and DL-threo-p-amino phenylserine specifically labeled chloramphenicol. On the basis of these results a pathway for the biosynthesis of chloramphenicol is presented. The lack of specific incorporation of 15N-nitrogen from a competitive feeding experiment in which both l5N-nitrate and 14N-DL-serine were fed to growing cultures suggests that both the amido- and the nitro-nitrogen atom present in this antibiotic are derived from a common pool. Studies on the enzyme, DAHP synthetase, show that in streptomyces sp. 3022a it is not subject to feed back inhibition by either phenylalanine, tyrosine, or chloramphenicol.
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  • 41
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    Biotechnology and Bioengineering 11 (1969), S. 1247-1254 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Red kidney bean (Phascolus vulgaris) cells, derived from roof, callus, were grown in suspension culture in shake flasks and in laboratory fermentors using batch and continuous batch culture techniques. The medium contained casein hydrolysate, sucrose, inorganic salts, vitamins, and growth hormones. In continuous batch culture yields of up to 171 g wet weight, (8.5 g dry weight) per liter were obtained in 7 days. Organic nitrogen was used preferentially. Growth on nitrate was considerably slower than on organic nitrogen sources. Indole acetic and naphthalene acetic acids were not essential for good growth of the cells whereas kinetin and 2, 4-D were. The optimum pH for growth was about p11 4.5. The presence of amylase and peroxidase was detected in culture filtrates. Amylase activity was low in either the presence or the absence of starch in the medium. Peroxidase production could be related directly with growth of the culture. Maximum peroxidase yield, as measured by the guaiacol method and expressed as horse radish peroxidase, was 1.25 × 10-8 M.
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  • 42
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    Biotechnology and Bioengineering 11 (1969), S. 1271-1284 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 2 Ill.
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  • 43
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    Biotechnology and Bioengineering 11 (1969), S. 1255-1270 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Some results of our studios on transformation of steroids by mixed culture fermentation are presented in this paper. Arthrobacter simplex was paired in turn with each of the following: Streptomyces roseochromogenes, Curvularia lunata, Absidia coerulea, and Aspergillus ochraceus. The steroid substrates examined for multiple transformation were 16α-hydroxy-cortexolone, 16α-hydroxy-cortexolone 16,17-acetonide, 9α-fluorohydrocortisone, 9α-fluorohydrocortisone 21-acetate, and 9α-fluorohydrocortisone 21-hemisuccinate. The effects of media, steroid substrate, and microbial interaction in a mixed culture on the induction and repression of steroid transforming enzymes were unique to each case studied. The reaction mechanism of the multiple steroid transformation was also found to vary from one mixed culture system to another. Two different reaction mechanisms were observed, namely, consecutive and parallel. In the former, one of the two enzymatic reactions always preceded the other, while in the latter, two different enzyme reactions occurred simultaneously, thereby giving rise to two different intermediates. Multiple transformation of steroids by a single step mixed culture fermentation has potential economic advantages.
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  • 44
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    Biotechnology and Bioengineering 11 (1969), S. 1289-1290 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
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  • 45
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    Biotechnology and Bioengineering 11 (1969), S. 1285-1287 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: One of the kinteic equations derived previously from a series of sophisticated batch and continuous alcohol fermentations by using a respiration-deficient mutant of baker's yeast is as follows: \documentclass{article}\pagestyle{empty}\begin{document}$$ {{dp} \mathord{\left/ {\vphantom {{dp} {dt}}} \right. \kern-\nulldelimiterspace} {dt}} = v_0 e^{ - k_2 p} \left[{{S \mathord{\left/ {\vphantom {S {\left({K_s ^\prime + S} \right)}}} \right. \kern-\nulldelimiterspace} {\left({K_s ^\prime + S} \right)}}} \right]X $$\end{document} where dp/dt = ethanol production rate, v0 = specific rate of ethanol production at p = 0, k2 = empirical constant, K′s = saturation constant, S = glucose concentration, and X = cell mass concentration. The above equation was confirmed in the previous paper to fit, the brewing of “sake.”The temperature of the specific brewing is not always constant (10 to 18°C). The effect of temperature on v0 was assessed from the Arrhenius plot, assuming that k2 was independent of temperature. Values of dp/dt taken from the “sake” brewing data were rearranged, taking the temperature change into account. These datu, corrected for the temperature, were found to follow quite favorably the kinetic equation mentioned above. So far, a prediction of the ethanol production rate in practice was rectified to the extent of p = 19%.
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  • 46
    ISSN: 0009-286X
    Keywords: Chemistry ; Industrial Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Neben verfahrenstechnischen Problemen sind bei der Süßwassergewinnung aus Meerwasser vor allem Werkstoff- und Korrosionsprobleme zu lösen, über die dieser Beitrag einen kurzen Überblick gibt. Die aufgezeigten Werkstoff-Fragen werden dabei unter den extremen Bedingungen thermischer Verfahren gesehen, die nach dem heutigen Stand der Technik am wirtschaftlichsten sind.
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  • 47
    ISSN: 0009-286X
    Keywords: Chemistry ; Industrial Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: In Ceuta (Span. Marokko) wurden zwei 24stufige Entspannungsverdampfer-Anlagen zur Gewinnung von Trinkwasser aus Meerwasser errichtet. Nachfolgend werden die Schwierigkeiten bei der Inbetriebnahme der Anlage und die erforderlichen Gegenmaßnahmen beschrieben. Durch kleine Änderungen konnten die Betriebssicherheit der Anlage und die Qualität des gewonnenen Wassers erheblich verbessert werden.
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  • 48
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    Chemie Ingenieur Technik - CIT 41 (1969) 
    ISSN: 0009-286X
    Keywords: Chemistry ; Industrial Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
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  • 49
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    Chemie Ingenieur Technik - CIT 41 (1969), S. 701-708 
    ISSN: 0009-286X
    Keywords: Chemistry ; Industrial Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: In einer früheren Veröffentlichung [1] wurde über den Stand der technischen Entwicklung auf dem Gebiet der Brennstoffelemente mit gasförmigen Brennstoffen berichtet. Als Ergänzung dazu beschäftigt sich die vorliegende Arbeit mit Batterien, die flüssige Brennstoffe umsetzen. Teil II, 1 behandelt die Methanol- und Formiat-Batterien, während Teil II, 2 die Hydrazin-Batterien beschreibt. Auch hier sollen wieder nur Batterien erwähnt werden, die technisch so weit entwickelt sind, daß sie bereits im praktischen Betrieb benutzt werden oder verwendet werden können. Die heute möglichen und die in Zukunft denkbaren Anwendungen dieser neuen elektrochemischen Stromquellen sollen in einer abschließenden Arbeit (Teil III) diskutiert werden.
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  • 50
    ISSN: 0009-286X
    Keywords: Chemistry ; Industrial Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Der Einfluß des Druckes auf die Reaktionsgeschwindigkeitskonstante läßt sich bei der Berechnung von Hochdruckreaktoren mit Hilfe des charakteristischen Aktivierungsvolumens der Reaktion erfassen. Die Änderung der Geschwindigkeitskonstante mit dem Druck wird diskutiert und ihr Einfluß auf den in einem Reaktor erzielbaren chemischen Umsatz sowie auf die Qualität der Reaktionsprodukte untersucht. Für die Ermittlung des charakteristischen Aktivierungsvolumens wird eine neue Methode angegeben.
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  • 51
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    Chemie Ingenieur Technik - CIT 41 (1969), S. 721-724 
    ISSN: 0009-286X
    Keywords: Chemistry ; Industrial Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Der Fehler, der bei der Messung des mittleren dynamischen Drucks und der daraus berechneten mittleren Geschwindigkeit auftritt, ist sowohl abhängig von der Turbulenzstruktur selbst als auch von der Form der verwendeten Sonde. Der durch die Sonde hervorgerufene Fehler läßt sich durch die Richtungsempfindlichkeit in nicht turbulenter Strömung erklären und berechnen, sofern die Turbulenzstruktur selbst bekannt ist. Untersuchungen an einem turbulenten Luftfreistrahl konstanter Dichte werden beschrieben. Der Meßfehler in Strömungen mit Geschwindigkeits- und Dichteschwankungen wird qualitativ behandelt.
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  • 52
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    Chemie Ingenieur Technik - CIT 41 (1969), S. 725-731 
    ISSN: 0009-286X
    Keywords: Chemistry ; Industrial Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Es werden Methoden zur Ermittlung der Struktur von teilchenhaltigen Bindemittelschichten am Modell zinkoxid-haltiger Siliconharz-Schichten, wie sie in der Elektrophotographie Verwendung finden, beschrieben. Es zeigt sich, daß Schichten, die schlecht dispergiertes Zinkoxid enthalten, zur Porosität neigen. Aufgrund der Untersuchungen können Aussagen über den Mechanismus gemacht werden, der zum Entstehen der Poren führt. Über zellartige Strukturen, die im Zusammenhang mit den Poren beobachtet worden sind, wird berichtet. Es wird darauf hingewiesen, daß die gefundenen Strukturen nicht nur bei zinkoxid-haltigen Siliconharzen, sondern auch bei anderen Systemen auftreten können. Möglichkeiten zur Vermeidung von Poren werden angegeben.
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  • 53
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    Chemie Ingenieur Technik - CIT 41 (1969), S. 736-736 
    ISSN: 0009-286X
    Keywords: Chemistry ; Industrial Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
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  • 54
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    Chemie Ingenieur Technik - CIT 41 (1969), S. 739-739 
    ISSN: 0009-286X
    Keywords: Chemistry ; Industrial Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
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  • 55
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    Chemie Ingenieur Technik - CIT 41 (1969), S. 740-740 
    ISSN: 0009-286X
    Keywords: Chemistry ; Industrial Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
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  • 56
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    Chemie Ingenieur Technik - CIT 41 (1969) 
    ISSN: 0009-286X
    Keywords: Chemistry ; Industrial Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
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  • 57
    ISSN: 0009-286X
    Keywords: Chemistry ; Industrial Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Zur Bestimmung reaktionskinetischer Daten von Simultanreaktionen erster Ordnung bei der isothermen Pyrolyse von Feststoffen, hier speziell von Kohlen, wurde ein Analog-Rechenverfahren entwickelt. Die berechneten Reaktionsgeschwindigkeitskonstanten lassen sich unabhängig vom Inkohlungsgrad bestimmten Reaktionsstufen zuordnen. Für die bei der Pyrolyse von Steinkohlen anfallenden Entgasungsprodukte Methan, Propan, Äthylen und Kohlenmonoxid wurden Aktivierungsenergien von 48 bis 66 kcal/mol und Stoßfaktoren von 1013 bis 1017 min-1 ermittelt. Für Äthan liegen die Werte bei 50 bis 90 kcal/mol und 1014 bis 1025 min-1 Miteinem weiteren Analog-Rechenprogramm können aus den reaktionskinetischen Daten, die mit dem ersten Rechenprogramm ermittelt wurden, die ihnen entsprechenden nicht-isotherm aufgenommenen Entgasungskurven berechnet werden.
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  • 58
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    Chemie Ingenieur Technik - CIT 41 (1969), S. 196-198 
    ISSN: 0009-286X
    Keywords: Chemistry ; Industrial Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Es wird eine Übersicht gegeben über die Möglichkeiten der elektrischen Beheizung von Eisenerz-Verhüttungsöfen. Bei Koks-Heizung geht ein beträchtlicher Teil der Wärmeenergie ungenutzt mit den Gichtgasen verloren. Dieser Verlust ist bei elektrisßher Heizung sehr viel geringer.
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  • 59
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    Chemie Ingenieur Technik - CIT 41 (1969), S. 199-204 
    ISSN: 0009-286X
    Keywords: Chemistry ; Industrial Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Es wird gezeigt, unter welchen Bedingungen heutzutage die Erzeugung von Calciumcarbid und Carbid-Acetylen konkurrenzfähig ist mit der petrochemischen Erzeugung von Acetylen. Ferner wird dargelegt, wann andere Verfahren mit Äthylen als Rohstoff solche auf Acetylen-Basis ablösen. Eine wesentliche Entwicklung, die die Konkurrenzfähigkeit von Carbid-Acetylen vorteilhaft beeinflußt, ist die der Hohlelektrode. Für einige Anwendungsgebiete werden sich daher bei günstigen Voraussetzungen Calciumcarbid und Carbid-Acetylen behaupten können.
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  • 60
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    Chemie Ingenieur Technik - CIT 41 (1969), S. 777-787 
    ISSN: 0009-286X
    Keywords: Chemistry ; Industrial Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
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  • 61
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    Chemie Ingenieur Technik - CIT 41 (1969), S. 773-775 
    ISSN: 0009-286X
    Keywords: Chemistry ; Industrial Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Die Zündgrenzen des Systems NH3/N2/O2 wurden bei mehreren Temperaturen in Abhängigkeit von der Zusammensetzung unter einem Druck von 130 atü bestimmt. Bei Extrapolation der Ergebnisse zeigt sich, daß Gemische mit weniger als 7, 5 Mol-% NH3 (bei 50°C) und weniger als 11 Mol-% O2 (bei 110 bis 120°C) sowie mehr als 67 Mol-% N2 (bei 50°C) nicht mehr zündbar sind. Durch Temperatursteigerung werden die Zündgrenzen erweitert. Bei den Explosionen wurden Drucksteigerungen etwa bis zum 10fachen des Anfangsdruckes beobachiet.
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    Chemie Ingenieur Technik - CIT 41 (1969), S. 787-789 
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    Keywords: Chemistry ; Industrial Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
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    Chemie Ingenieur Technik - CIT 41 (1969) 
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    Keywords: Chemistry ; Industrial Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
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    Chemie Ingenieur Technik - CIT 41 (1969), S. 226-226 
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    Keywords: Chemistry ; Industrial Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
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    Chemie Ingenieur Technik - CIT 41 (1969), S. 227-228 
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    Keywords: Chemistry ; Industrial Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
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    Chemie Ingenieur Technik - CIT 41 (1969), S. 832-834 
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    Keywords: Chemistry ; Industrial Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
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    Chemie Ingenieur Technik - CIT 41 (1969), S. 837-837 
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    Keywords: Chemistry ; Industrial Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
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    Chemie Ingenieur Technik - CIT 41 (1969), S. 838-839 
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    Keywords: Chemistry ; Industrial Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
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    Chemie Ingenieur Technik - CIT 41 (1969), S. 839-841 
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    Source: Wiley InterScience Backfile Collection 1832-2000
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  • 70
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    Chemie Ingenieur Technik - CIT 41 (1969), S. 591-592 
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    Source: Wiley InterScience Backfile Collection 1832-2000
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    Chemie Ingenieur Technik - CIT 41 (1969), S. 592-592 
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    Keywords: Chemistry ; Industrial Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
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  • 72
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    Chemie Ingenieur Technik - CIT 41 (1969), S. 988-989 
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    Keywords: Chemistry ; Industrial Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 2 Ill.
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  • 73
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    Source: Wiley InterScience Backfile Collection 1832-2000
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    Notes: Es werden die Erfahrungen und Betriebsergebnisse seit der Inbetriebnahme des ersten Kessels der Müllverbrennungsanlage in Berlin-Ruhleben mitgeteilt, und es wird dargelegt, in welcher Weise sie für die Planung des nächsten Bauabschnitts ausgewertet wurden. Einzelne wichtige Erkenntnisse werden sich jedoch erst beim Bau weiterer Anlagen verwirklichen lassen.
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  • 74
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    Chemie Ingenieur Technik - CIT 41 (1969), S. 1015-1020 
    ISSN: 0009-286X
    Keywords: Chemistry ; Industrial Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Es werden der Druckverlust und der Stoffübergang in einem Strahlapparat untersucht, in dem die Flüssigkeit an der Abströmkante eines Konus zerstäubt wird. Im Gegensatz zum Venturi-Absorber wird dabei die zum Zerstäuben notwendige Energie nur von dem Gasstrom aufgebracht. Die Konstruktionen werden dadurch einfach und betriebssicher. Aufgrund einer Dimensionsanalyse können die Meßergebnisse für verschiedene geometrische Abmessungen des Apparates und für verschiedene Stoffsysteme in großen Bereichen durch dimensionslose Beziehungen beschrieben werden.
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  • 75
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    Chemie Ingenieur Technik - CIT 41 (1969), S. 1007-1014 
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    Keywords: Chemistry ; Industrial Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Es wurde eine Apparatur entwickelt, die differentialthermoanalytische Messungen bis zu Drücken von 2500 at bei Temperaturen bis 300°C durchzuführen gestattet. Für die reaktionskinetische Auswertung solcher DTA-Messungen werden zwei Methoden vorgeschlagen, die auch bei komplexen Reaktionen anwendbar sind. Die eine beruht auf der direkten Bestimmung der kinetischen Parameter aus Temperatur- und Umsatzkurven für spezielle Reaktionsabschnitte, die andere auf der Nachrechnung der experimentell ermittelten Umsatzverläufe. Nach beiden Methoden wurden für die Äthylen-Hochdruckpolymerisation die Stoßfaktoren und Aktivierungsenergien der Teilreaktionen (Start, Wachstum und Abbruch) ermittelt und diese mit früher nach anderen Meßverfahren gewonnenen Ergebnissen verglichen.
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    Chemie Ingenieur Technik - CIT 41 (1969), S. 1022-1023 
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    Keywords: Chemistry ; Industrial Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
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    Additional Material: 3 Ill.
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    Chemie Ingenieur Technik - CIT 41 (1969), S. 1023-1024 
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    Source: Wiley InterScience Backfile Collection 1832-2000
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    Additional Material: 2 Ill.
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    Chemie Ingenieur Technik - CIT 41 (1969), S. 1040-1042 
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    Source: Wiley InterScience Backfile Collection 1832-2000
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    Chemie Ingenieur Technik - CIT 41 (1969), S. 1037-1039 
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    Source: Wiley InterScience Backfile Collection 1832-2000
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    Chemie Ingenieur Technik - CIT 41 (1969) 
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    Notes: Für die Luftführung in Reinräumen ist eine turbulenzarme Verdrängungsströmung (Laminar Flow) der turbulenten Strahlbelüftung vorzuziehen, obgleich auch sie einen gewissen Partikeltransport quer zur Strömungsrichtung bewirkt. Die Ausbreitung der Kontamination wird durch die Bestimmung der Grenzkurve, d. h. der Umhüllenden eines sich hinter einer Kontaminationsquelle ausbildenden kontaminierten Bereiches, gekennzeichnet, die einen konstanten Winkel mit den Stromlinien aufweist. Somit kann, wenn das Stromlinienbild durch Rauchfaden-Photographie und die Lage der Kontaminationsquelle bekannt sind, der gefährdete kontaminierte Bereich abgeschätzt werden.
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    Chemie Ingenieur Technik - CIT 41 (1969), S. 638-638 
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    Source: Wiley InterScience Backfile Collection 1832-2000
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  • 83
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    Chemie Ingenieur Technik - CIT 41 (1969), S. 641-643 
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    Source: Wiley InterScience Backfile Collection 1832-2000
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  • 84
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    Source: Wiley InterScience Backfile Collection 1832-2000
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    Notes: Mit Hilfe einer neuartigen photometrischen Meßmethode kann die Verteilung des örtlichen Stoffüberganges an zwangsumströmten Körpern ermittelt werden. Strömungsmedium ist Luft, Ammoniak als übergehender Stoff wird an der Oberfläche der Körper absorbiert und chemisch gebunden. Derartige Messungen erlauben unter der Voraussetzung der Analogie von Wärme- und Stoffübertragung gleichzeitig Aussagen über die Verteilung des Wärmeüberganges. Die Untersuchung wurde an querangeströmten Kreiszylindern unterschiedlichen Durchmessers ausgeführt. Die Methode gestattet es, mit relativ einfachen Mitteln örtliche Stoffübergangszahlen innerhalb eines Streubereiches in der Größenordnung von ±5% zu bestimmen.
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    Chemie Ingenieur Technik - CIT 41 (1969), S. 1082-1091 
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    Source: Wiley InterScience Backfile Collection 1832-2000
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  • 86
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    Chemie Ingenieur Technik - CIT 41 (1969), S. 1092-1094 
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    Source: Wiley InterScience Backfile Collection 1832-2000
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  • 87
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    Chemie Ingenieur Technik - CIT 41 (1969), S. 1095-1099 
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    Keywords: Chemistry ; Industrial Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Die in der Praxis vorkommenden Diffusionsflammen lassen sich häufig auf die Grundformen Einzelstrahlflamme, konzentrische Doppelstrahlflamme und Drallstrahlflamme zurückführen. Die Beschreibung der Strahlmischung mit Hilfe der Ähnlichkeitsgesetze turbulenter Strahlen erlaubt, den Einfluß der Anfangsbedingungen am Brenner auf die weiter stromab liegenden Gebiete systematisch zu erfassen. In begrenztem Umfang liefert die Ähnlichkeitstheorie auch Aussagen über Flammen, die in Gruppen angeordnet sind, und über Flammen, die sich unter Ausbildung eines Rückstromwirbels in begrenzten Feuerräumen ausbreiten. Bei Anwendung der Ähnlichkeitsgesetze ergeben sich Parameter, die es ermöglichen, den Umfang der experimentellen Untersuchungen einzuschränken.
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  • 88
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    Chemie Ingenieur Technik - CIT 41 (1969), S. 1100-1105 
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    Keywords: Chemistry ; Industrial Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Zur Klärung des Zeitverhaltens einer Füllkörperkolonne bei der Rektifikation eines ternären Systems wurden sprungförmige Störungen im Flüssigkeits/Dampf-Verhältnis vorgenommen. Die Kolonne bestand aus einem Verstärkerteil und wurde kontinuierlich betrieben. Als Testgemisch diente das nahezu ideale System Äthylacetat/Benzol/Trichloräthylen. Die Zeitkonstanten, die das Abklingen der Störungen charakterisieren, wurden gemessen sowie Übergangsfunktionen und Konzentrationsprofile beschrieben.
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  • 89
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    Chemie Ingenieur Technik - CIT 41 (1969), S. 1111-1118 
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    Keywords: Chemistry ; Industrial Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Der Sauerstoff-Austausch zwischen einer mit dem Isotop 18O angereicherten Wasserprobe und strömendem CO2-Gas mit natürlicher Isotopenhäufigkeit läßt sich durch eine Zeitkonstante charakterisieren, die durch Messung des zeitlichen Verlaufs der 18O-Konzentration im Wasser bestimmt wird. Aus der Abhängigkeit der Zeitkonstante von der Temperatur, vom CO2-Durchsatz, vom Bicarbonat- und Carbonat-Gehalt des Wassers werden die Geschwindigkeitskonstanten der Austauschreaktionen ermittelt.
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    Chemie Ingenieur Technik - CIT 41 (1969), S. 1173-1176 
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    Keywords: Chemistry ; Industrial Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Für die pneumatische Förderung von feinkörnigen Gütern wird ein Bypass-System beschrieben, welches die Anwendung einer Pfropfenströmung zur Förderung ohne Verstopfungsgefahr erlaubt. Auf diese Weise wird der Luftverbrauch und damit der Leistungsbedarf erheblich gesenkt, und durch Auswechseln der Förderleitung kann mit den vorhandenen Kompressoren eine höhere Förderkapazität erreicht werden.
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  • 91
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    Source: Wiley InterScience Backfile Collection 1832-2000
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    Notes: Es werden Berechnungsgleichungen zur Pfropfen- und Ballenförderung aufgestellt, die sich zur raschen Bewältigung ausgedehnter Versuchs- und Entwicklungsarbeiten bereits gut bewährt haben. Grundlagenversuche mit einer für variablen Druck bis 25 at und Gutbeladungen bis 300 eingerichteten Versuchsanlage führten unter Verwendung älterer Ergebnisse von M. Bohnet zu einer neuen Theorie der Strähnenförderung. Diese erlaubt eine einfache und schnelle Berechnung pneumatischer Förderleitungen mit ausreichender Genauigkeit für praktische Anwendungen.
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  • 92
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    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Der Einfluß von erzwungenen Geschwindigkeitsschwankungen auf den Stoffübergang zwischen Rohrwand und Gas bei ausgebildeter laminarer Strömung wurde am Beispiel einer stofftransportbestimmten heterogenen katalytischen Reaktion in einem Strömungsrohr untersucht. Die Versuche wurden mit unterschiedlichen Frequenzen im Bereich von 275 Hz bis 1325 Hz und Schalldrucken bis 165,7 dB durchgeführt. Bei Schalleinwirkung nahm die effektive Reaktionsgeschwindigkeit wegen der Erhöhung des Stoffüberganges zu. Der Effekt vergrößerte sich mit zunehmendem Schalldruck und abnehmender Frequenz der Schallwellen. Eine empirische Beziehung, die die Abhängigkeit der Erhöhung der Stoffübergangszahl von Frequenz, Schalldruck und mittlerer Gasgeschwindigkeit im Rohrreaktor angibt, wurde aufgestellt.
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    Chemie Ingenieur Technik - CIT 41 (1969), S. 834-836 
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    Keywords: Chemistry ; Industrial Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
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    Chemie Ingenieur Technik - CIT 41 (1969), S. 838-839 
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    Keywords: Chemistry ; Industrial Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
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  • 95
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    Chemie Ingenieur Technik - CIT 41 (1969), S. 1212-1221 
    ISSN: 0009-286X
    Keywords: Chemistry ; Industrial Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Es wird eine Anleitung für den richtigen Einbau von flammendurchschlagsicheren Armaturen in Tankanlagen mit brennbaren Flüssigkeiten gegeben. Nach allgemein gültigen Grundlagen wird die Absicherung von Behälterlüftungen und Anschlußrohrleitungen besprochen. Ferner werden weitere Tankarmaturen behandelt, die flammendurchschlagsicher, gasdicht und funkenfrei sein müssen. Der Einbau von Flammenschutzsicherungen bei Motoren wird kurz behandelt. Die Auslegung von Armaturen und ihre Wartung werden ausführlich dargelegt.
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    Chemie Ingenieur Technik - CIT 41 (1969), S. 849-855 
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    Keywords: Chemistry ; Industrial Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Die Schwingmahlung eines olivin-reichen Titanomagnetits führte nach einer Mahldauer von 320 h zu einer nahezu vollständigen Lösbarkeit des Titan- und des Eisen-Anteils. Weiterhin wurde für die Feinstmahlung eine allgemein gültige Abhängigkeit zwischen Mahlungsgleichgewicht und Mahlbarkeitsgrenze gefunden. Außerdem ließen sich Rekristallisationen während des Mahlprozesses nachweisen.
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    Notes: An einem längs angeströmten, von innen beheizbaren Platin-Rohr wird die katalytische Oxidation von Kohlenoxid mit Sauerstoff untersucht. Das Platin-Rohr bildet die innere Begrenzung eines Ringspaltes, dessen Wärmeübergang in Vorversuchen gemessen wird. Der Strömungszustand wird im Bereich der Reynolds-Zahl 110 ≤ Re ≤ 1,2 · 104 geändert; das Gasgemisch enthält 2 bis 7 Vol.-% Kohlenoxid in Sauerstoff. Bei der Beaufschlagung der Apparatur mit dem Reaktionsgas können stabile Betriebspunkte gemessen werden, die sich befriedigend durch ein Stabilitätsdiagramm der Reaktion erklären lassen.
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    Chemie Ingenieur Technik - CIT 41 (1969), S. 868-877 
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    Keywords: Chemistry ; Industrial Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Für die wichtigsten Modelle homogener chemischer Rohrreaktoren werden konsistente und numerisch stabile finite Differenzengleichungen angegeben. Als spezielles Rechenbeispiel wurde der nichtisotherme Rohrreaktor für Gasphasereaktionen ausgewählt. Das Übergangsverhalten dieses technisch wichtigen Reaktortyps konnte mit hoher Genauigkeit und relativ geringer Rechenzeit ermittelt werden.
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    Chemie Ingenieur Technik - CIT 41 (1969), S. 882-884 
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    Keywords: Chemistry ; Industrial Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
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    Weinheim : Wiley-Blackwell
    Chemie Ingenieur Technik - CIT 41 (1969) 
    ISSN: 0009-286X
    Keywords: Chemistry ; Industrial Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
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