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  • 1990-1994  (260)
  • 1985-1989
  • 1910-1914
  • 1990  (260)
  • Biochemistry  (136)
  • Life Sciences  (124)
  • 1
    Electronic Resource
    Electronic Resource
    Histochemical and biochemical plasticity of muscle fibers in the little brown bat (Myotis lucifugus) (1990)
    Springer
    Journal of comparative physiology 160 (1990), S. 183-186 
    Details
    ISSN: 1432-136X
    Keywords: Histology ; Biochemistry ; Muscle physiology ; Hibernations ; Metabolism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Fiber composition, and glycolytic and oxidative capacities of the pectoralis, gastrocnemius, and cardiac muscles from active and hibernating little brown bats (Myotis lucifugus) was studied. The data were used to test two hypotheses: First, since hibernating bats maintain the capability of flight and make use of leg muscles to maintain a roosting position all winter, the fiber composition of the pectoralis and gastrocnemius muscles should not change with season. Second, we tested the hypothesis of Ianuzzo et al. (in press), who propose that the oxidative potential of mammalian cardiac muscle should increase with increasing heart rate while glycolytic potential should not. Our results indicate that the fiber composition of the pectoralis muscle was uniformly fast-twitch oxidative (FO)_ regardless of the time of year, as predicted. However, the gastrocnemius muscle exhibited a change in FO composition from 83% in active to 61% in hibernating animals. Contrary to the variable change in histochemical properties with metabolic state, a trend of reduced maximal oxidative (CS) and glycolytic (PFK) potential during hibernation in both flight and leg muscles was apparent. The oxidative potential of flight and leg muscles decreased by 15.2% and 56.5%, respectively, while the glycolytic potential of the same muscles decreased by 23.5% and 60.5%, respectively. As predicted, the glycolytic potential of cardiac muscle remained constant between active and hibernating bats, although there was a significant decrease (22.0%) in oxidative potential during hibernation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00300951
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  • 2
    Electronic Resource
    Electronic Resource
    Masthead (1990)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Computational Chemistry 11 (1990) 
    Details
    ISSN: 0192-8651
    Keywords: Computational Chemistry and Molecular Modeling ; Biochemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Computer Science
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcc.540110101
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  • 3
    Electronic Resource
    Electronic Resource
    SCF, MP2, and CEPA-1 calculations on the OH ‥ O hydrogen bonded complexes (H2O)2 and (H2O-H2CO) (1990)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Computational Chemistry 11 (1990), S. 1-18 
    Details
    ISSN: 0192-8651
    Keywords: Computational Chemistry and Molecular Modeling ; Biochemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Computer Science
    Notes: Counterpoise corrected ab initio calculations are reported for (H2O)2 and H2O-H2CO. Geometry searches were done in the moment-optimized basis DZP' at the SCF, MP2, and CEPA-1 levels of theory, followed by more accurate single-point calculations in basis ESPB, which includes bondfunctions to saturate the dispersion energy. The final equilibrium binding energies obtained are -4.7 ±0.3 kcal/mol for a near-linear (H2O)2 structure and -4.6 ±0.3 kcal/mol for a strongly bent HOH ‥ OCH2 structure. The energy difference between these systems is much smaller than in all previous ab initio work. Cyclic (C2h) and bifurcated (C2v) transition structures for (H2O)2 are located at 1.0 ±0.1 kcal/mol and 1.9 ±0.3 kcal/mol above the global minimum, respectively. A new partitioning scheme is presented that rigorously partitions the MP2 correlation interaction energy in intra and intermolecular (dispersion) contributions. These terms are large (up to 2 kcal/mol) but of opposite sign for most geometries studied and hence their overall effect upon the final structures is relatively small. The relative merits of the MP2 and CEPA-1 approaches are discussed are discussed and it is concluded that for economical reasons MP2 is to be preferred, especially for larger systems.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcc.540110102
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  • 4
    Electronic Resource
    Electronic Resource
    A data compression method applicable to first-order convergent iterative procedures (1990)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Computational Chemistry 11 (1990), S. 45-57 
    Details
    ISSN: 0192-8651
    Keywords: Computational Chemistry and Molecular Modeling ; Biochemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Computer Science
    Notes: A data compression method is presented that is generally applicable to first-order convergent iterative procedures that employ subspace expansions or extrapolations based on successive correction vectors. This method is based on the truncation of insignificant information in successive correction vectors. Although the correction vectors themselves may be severely truncated with the proposed approach, the final solution vector may be represented to arbitrary accuracy. A feature of the proposed method is that more slowly convergent iterative procedures allow the correction vectors to be more severely truncated without affecting the overall convergence rate. The method is implemented and applied to the iterative Davidson diagonalization method. If the compressed representation of the expansion vectors can be held in main computer memory, then a significant reduction in the I/O requirements is achieved.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcc.540110105
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  • 5
    Electronic Resource
    Electronic Resource
    Partial optimization of large molecules and clusters (1990)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Computational Chemistry 11 (1990), S. 67-75 
    Details
    ISSN: 0192-8651
    Keywords: Computational Chemistry and Molecular Modeling ; Biochemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Computer Science
    Notes: By examining the displacement coordinate metric three modes of constrained optimization for large molecules and clusters are suggested. The first method corresponds to a conventional optimization using internal coordinates. The second mode has applications with respect to both internal and cartesian coordinates. The final mode is particularly interesting because it can result in computational savings. A mixture of both internal and cartesian coordinates is specified where these coordinates are usually a subset of the molecules or clusters total coordinate set. In the optimization only a subset of the energy derivatives need be evaluated reducing the computational effort associated with the gradient calculation.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcc.540110108
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  • 6
    Electronic Resource
    Electronic Resource
    Calculation of the nonlinear optical properties of molecules (1990)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Computational Chemistry 11 (1990), S. 82-87 
    Details
    ISSN: 0192-8651
    Keywords: Computational Chemistry and Molecular Modeling ; Biochemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Computer Science
    Notes: A finite-field method for the calculation of polarizabilities and hyperpolarizabilities is developed based on both an energy expansion and a dipole moment expansion. This procedure is implemented in the MOPAC semiempirical program. Values and components of the dipole moment (μ), polarizability (α), first hyperpolarizability (β), and second hyperpolarizability (γ) are calculated as an extension of the usual MOPAC run. Applications to benzene and substituted benzenes are shown as test cases utilizing both MNDO and AM1 Hamiltonians.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcc.540110110
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  • 7
    Electronic Resource
    Electronic Resource
    Protein structure prediction using a combination of sequence homology and global energy minimization I. Global energy minimization of surface loops (1990)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Computational Chemistry 11 (1990), S. 121-151 
    Details
    ISSN: 0192-8651
    Keywords: Computational Chemistry and Molecular Modeling ; Biochemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Computer Science
    Notes: A procedure has been developed for global energy minimization of surface loops of proteins in the presence of a fixed core. The ECEPP potential function has been modified to allow more accurate representations of hydrogen bond interactions and intrinsic torsional energies. A computationally efficient representation of hydration free energy has been introduced. A local minimization procedure has been developed that uses a cutoff distance, minimization with respect to subsets of degrees of freedom, analytical second derivatives, and distance constraints between rigid segments to achieve efficiency in applications to surface loops. Efficient procedures have been developed for deforming segments of the initial backbone structure and for removing overlaps. Global energy minimization of a surface loop is accomplished by generating a sequence (or a trajectory) of local minima, the component steps of which are generated by searching collections of local minima obtained by deforming seven-residue segments of the surface loop. The search at each component step consists of the following calculations: (1) A large collection of backbone structures is generated by deforming a seven-residue segment of the initial backbone structure. (2) A collection of low-energy backbone structures is generated by applying local energy minimization to the resulting collection of backbone structures (interactions involving side chains that will be searched in this component step are not included in the energy). (3) One low-energy side-chain structure is generated for each of the resulting low-energy backbone structures. (4) A collection of low-energy local minima is generated by applying local energy minimization to the resulting collection of structures. (5) The local minimum with the lowest energy is retained as the next point of the trajectory. Applications of our global search procedure to surface segments of bovine pancreatic trypsin inhibitor (BPTI) and bovine trypsin suggest that component-step searches are reasonably complete. The computational efficiency of component-step searches is such that trajectories consisting of about 10 component steps are feasible using an FPS-5200 array processor. Our procedure for global energy minimization of surface loops is being used to identify and correct problems with the potential function and to calculate protein structure using a combination of sequence homology and global energy minimization.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcc.540110115
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  • 8
    Electronic Resource
    Electronic Resource
    Comparative study of the molecular electrostatic potential obtained from different wavefunctions. Reliability of the semiempirical MNDO wavefunction (1990)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Computational Chemistry 11 (1990), S. 416-430 
    Details
    ISSN: 0192-8651
    Keywords: Computational Chemistry and Molecular Modeling ; Biochemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Computer Science
    Notes: A systematic analysis of the molecular electrostatic potential (MEP) is presented. This study has been performed with a twofold purpose: first, to study the MEP dependence with regard to the quality of the basis set used to compute the ab initio SCF wavefunction and second, to develop and to assess a new strategy for computing isoelectrostatic potential maps using the semiempirical MNDO wavefunction. The only differences between this procedure and the ab initio SCF MEP computation lie in the freezing of the inner electrons and in the origin of the first-order density matrix. The statistical analysis of MEPs computed for a large number of molecules from MNDO wavefunction and ab initio SCF wavefunctions obtained using STO-3G, 4-31G, 6-31G, 4-31G*, 6-31G*, and 6-31G** basis sets points out the ability of any wavefunction to reproduce the general topological characteristics of the MEP surfaces. Nevertheless, split-valence basis sets including polarization functions are necessary to obtain accurate MEP minimum energy values. MNDO wavefunction tends to overestimate the MEP minima depth by a constant factor and shows an excellent ability to reflect the relative variation of MEP minima energies derived from a rather sophisticated (6-31G*) basis set, lacking of the shortcomings detected in the semiempirical CNDO approximation.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcc.540110403
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  • 9
    Electronic Resource
    Electronic Resource
    Dynamics of peptides with fixed geometry: Kinetic energy terms and potential energy derivatives as functions of dihedral angles (1990)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Computational Chemistry 11 (1990), S. 487-492 
    Details
    ISSN: 0192-8651
    Keywords: Computational Chemistry and Molecular Modeling ; Biochemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Computer Science
    Notes: Expressions are derived for computing the kinetic energy of a peptide with fixed geometry, in terms of dihedral angles as generalized coordinates; other expressions required for the solution of Lagrange's equations are also presented. The peptide is regarded as held stationary at one end. We also outline the computations that are needed in calculating the components of the third derivative of a potential energy function that consists of a sum of pairwise interatomic interactions.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcc.540110407
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  • 10
    Electronic Resource
    Electronic Resource
    Combined application of pair potentials and the MM2 force field for the modeling of ionophores (1990)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Computational Chemistry 11 (1990), S. 819-828 
    Details
    ISSN: 0192-8651
    Keywords: Computational Chemistry and Molecular Modeling ; Biochemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Computer Science
    Notes: Complexes of alkali and alkaline earth cations with organic compounds are modeled by describing ionligand interaction energies with pair potentials and intraligand as well as interligand energies with the MM2 potential. New pair potentials for the interaction of Li+, Na+, K+, Mg2+, and Ca2+ ions are derived on the basis of 30,000 ab initio interaction energy values with 70 selected model ligand molecules. Various problems of the combination of these two basically different potentials are discussed. An application for the K+ complex of 18-crown-6 is presented. For more flexible ligands the introduction of three-body correction terms of the pair potentials seems necessary.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcc.540110705
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  • 11
    Electronic Resource
    Electronic Resource
    Electronic structure and photoelectron spectra of Sb2 and Sb4 from local-spin-density calculations. Model potential for Sb (1990)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Computational Chemistry 11 (1990), S. 924-929 
    Details
    ISSN: 0192-8651
    Keywords: Computational Chemistry and Molecular Modeling ; Biochemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Computer Science
    Notes: The geometry, electronic structure, and photoelectron spectra of Sb2 and Sb4 have been investigated employing the LCGTO-MP-LSD method. For both molecules the geometrical and spectroscopic constants computed using the VWN potential are in very good agreement with the available experimental data. The HeI photoelectron spectra were computed with different potentials (Xα, GL, HL, BH, and VWN). The calculated spectra are in excellent agreement with the experimental ones and the use of the VWN potential gives the most accurate results. The reliable results for molecules complement those for the Sb atom and show that the proposed LSD Model Potential is able to give an accurate description of the structural and spectroscopic properties of the title molecules.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcc.540110804
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  • 12
    Electronic Resource
    Electronic Resource
    Monte Carlo simulations of liquid alkyl ethers with the OPLS potential functions (1990)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Computational Chemistry 11 (1990), S. 958-971 
    Details
    ISSN: 0192-8651
    Keywords: Computational Chemistry and Molecular Modeling ; Biochemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Computer Science
    Notes: Intermolecular potential functions have been developed for use in computer simulations of alkyl ethers. The simple OPLS model was adopted and parameterized to yield good descriptions of bimolecular and ion-molecule complexes as well as to reproduce experimental thermodynamic properties of liquid ethers. The principal testing featured Monte Carlo statistical mechanics simulations for liquid dimethyl ether (DME), ethyl methyl ether (EME), diethyl ether (DEE), and tetrahydrofuran (THF). Average errors of 1-3% are obtained for the computed densities and heats of vaporization including results for THF at pressures up to 5000 atm. The torsional motion about the central C—O bonds in EME and DEE was included in the simulations using rotational potential functions fit to results of molecular mechanics (MM2) calculations. The liquid-state environment is found to have negligible effect on the conformational equilibria.
    Additional Material: 15 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcc.540110808
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  • 13
    Electronic Resource
    Electronic Resource
    Concise, open-ended implementation of Rys polynomial evaluation of two-Electron integrals (1990)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Computational Chemistry 11 (1990), S. 972-977 
    Details
    ISSN: 0192-8651
    Keywords: Computational Chemistry and Molecular Modeling ; Biochemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Computer Science
    Notes: A quadrature-point-driven implementation of the standard Rys polynomial method for computing two-electron repulsion integrals of gaussian basis functions has been found to be both concise and openended with respect to the angular momentum of the gaussian functions (i.e., s,p,d,f,g,…). These are important features in certain applications, such as molecular properties and property gradients.
    Additional Material: 1 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcc.540110809
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  • 14
    Electronic Resource
    Electronic Resource
    Free energy perturbation calculations on models of active sites: Applications to adenosine deaminase inhibitors (1990)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Computational Chemistry 11 (1990), S. 994-1002 
    Details
    ISSN: 0192-8651
    Keywords: Computational Chemistry and Molecular Modeling ; Biochemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Computer Science
    Notes: Free energy perturbation calculations were performed to determine the free energy of binding associated with the presence of perhaps an unusual hydroxyl group in the transition state analog of nebularine, an inhibitor of the enzyme adenosine deaminase. The presence of a single hydroxyl group in this inhibitor has been found to contribute -9.8 kcal/mol to the free energy of binding, with a 108-fold increase in the binding affinity by the enzyme. In this work, we calculate the difference in solvation free energy for the 1,6-dihydropurine complex versus that of the 6-hydroxyl-1,6-dihydropurine complex to determine if this marked increase in binding affinity is attributed to an unusually hydrophobic hydroxyl group. The calculated ΔG associated for the solvation free energy is -11.8 kcal/mol. This large change in the solvation free energy suggests that this hydroxyl is instead unusually hydrophilic and that the difference in free energy of interaction for the two inhibitors to the enzyme must be at least ca. 20 kcal/mol. Although the crystal structure for adenosine deaminase is currently not known, we attempt to mimic the nature of the active site by constructing models which simulate the enzyme-inhibitor complex. We present a first attempt at determining the change in free energy of binding for a system in which structural data for the enzyme is incomplete. To do this, we construct what we believe is a minimal model of the binding between adenosine deaminase and an inhibitor. The active site is simulated as a single charged carboxyl group which can form a hydrogen bond with the hydroxyl group of the analog. Two different carboxyl anion models are used. In the first model, the association is modeled between an acetic acid anion and the modified inhibitor. The second model consists of a hydrophobic amino acid pocket with an interior Glu residue in the active site. From these models we calculate the change in free energy of association and the overall change in free energy of binding. We calculate the free energies of interaction both in the absence and presence of water. We conclude from this that the presence of a single suitably placed-CO-2 group probably cannot explain the binding effect of the-OH group and that additional interactions will be found in the adenosine deaminase active site.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcc.540110811
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  • 15
    Electronic Resource
    Electronic Resource
    Pseudopotential calculations for methyl compounds of zinc and magnesium (1990)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Computational Chemistry 11 (1990), S. 1029-1037 
    Details
    ISSN: 0192-8651
    Keywords: Computational Chemistry and Molecular Modeling ; Biochemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Computer Science
    Notes: Pseudopotentials and valence basis sets to be used in calculations for organometallic compounds of zinc and magnesium have been tested in calculations for the M(CH3)n (M = Zn, Mg; n = 1,2) molecules. Valence correlation effects are treated at the SDCI and CEPA levels. The capability of a polarization potential on zinc to account for the valence shell contracting effect of core valence correlation is studied. Properties considered are geometries, force constants, Mulliken populations, ionization potentials, atomization, and binding energies. Differences in bonding between the two dimethyl compounds are discussed.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcc.540110905
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  • 16
    Electronic Resource
    Electronic Resource
    AM1 study of acid-catalyzed hydrolysis of maleamic (4-amino-4-oxo-2-butenoic) acids (1990)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Computational Chemistry 11 (1990), S. 1094-1100 
    Details
    ISSN: 0192-8651
    Keywords: Computational Chemistry and Molecular Modeling ; Biochemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Computer Science
    Notes: The acid-catalyzed hydrolysis mechanisms of maleamic (4-amino-4-oxo-2-butenoic) acids were studied using AM1 method. The reaction proceeded mainly in two steps: (1) nucleophilic attack of the undissociated carboxyl group on the adjacent aminocarbonyl carbon via a zwitterionic intermediate; and (2) the rate-determining proton transfer to form the zwitterionic tetrahedral intermediate. In each step, the hydration of water and hydronium ion molecules was important in stabilizing the polarized intermediates. The substituent effects at the amide moiety and the 2,3-positions of the maleamic acids were qualitatively estimated for each step.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcc.540110913
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  • 17
    Electronic Resource
    Electronic Resource
    Masthead (1990)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Computational Chemistry 11 (1990) 
    Details
    ISSN: 0192-8651
    Keywords: Computational Chemistry and Molecular Modeling ; Biochemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Computer Science
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcc.540111001
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  • 18
    Electronic Resource
    Electronic Resource
    The application of molecular similarity calculations (1990)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Computational Chemistry 11 (1990), S. 1139-1146 
    Details
    ISSN: 0192-8651
    Keywords: Computational Chemistry and Molecular Modeling ; Biochemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Computer Science
    Notes: A prescription for applying the method of molecular similarity calculations based on electrostatic potentials and fields is developed by consideration of a typical structure-activity series. Firm conclusions are drawn about the nature of the grid of points surrounding the molecules and about the choice of geometry, but options for point charges are less clearcut.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcc.540111004
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  • 19
    Electronic Resource
    Electronic Resource
    Combinatorial models and algorithms in chemistry. The expanded Wiener number - a novel topological index (1990)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Computational Chemistry 11 (1990), S. 899-908 
    Details
    ISSN: 0192-8651
    Keywords: Computational Chemistry and Molecular Modeling ; Biochemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Computer Science
    Notes: An expanded form of the Wiener number is suggested for characterization of molecular graphs and structure-property correlations. The simple, computer-oriented method for counting of the novel index is briefly discussed.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcc.540110802
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  • 20
    Electronic Resource
    Electronic Resource
    Automated conformational analysis: Directed conformational search using the A* algorithm (1990)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Computational Chemistry 11 (1990), S. 1193-1205 
    Details
    ISSN: 0192-8651
    Keywords: Computational Chemistry and Molecular Modeling ; Biochemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Computer Science
    Notes: A common requirement in conformational analysis is the identification of a molecule's lowest energy conformations. The application of the A* algorithm to this problem is examined. The algorithm uses heuristic information about the problem domain to direct the search and has been implemented in a system for performing automated conformational analysis. The method is detailed and sample results presented. Some limitations of the approach are identified.
    Additional Material: 13 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcc.540111012
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  • 21
    Electronic Resource
    Electronic Resource
    Correlation of the acidity of substituted phenols, anilines, and benzoic acids calculated by MNDO, AM1, and PM3 with Hammett-type substituent constants (1990)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Computational Chemistry 11 (1990), S. 1009-1016 
    Details
    ISSN: 0192-8651
    Keywords: Computational Chemistry and Molecular Modeling ; Biochemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Computer Science
    Notes: Heats of formation and net atomic charges of some 120 structures involving substituted phenols, anilines, and benzoic acids and the corresponding anions were calculated by MNDO, AM1, and PM3 semiempirical methods. The gas phase acidities of substituted phenols and anilines and the net atomic charges on the anionic heteroatoms of the corresponding anions have been successfully correlated with σ- constants. Moreover, good correlations with σ were found for the charges on the acidic hydrogens of substituted phenols and anilines. In contrast, the gas phase acidities of substituted benzoic acids and the charges on the anionic oxygens of the corresponding anions are better correlated with Taft σ° constants. Comparisons of these results with experimental data and ab initio theoretical calculations indicate that AM1 and PM3 methods are much better than MNDO in predicting the acidity of aromatic compounds.
    Additional Material: 8 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcc.540110902
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  • 22
    Electronic Resource
    Electronic Resource
    Comparative study of imidazole hydration: Ab initio and electrostatic calculations vs. Cambridge structural database analysis (1990)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Computational Chemistry 11 (1990), S. 1038-1046 
    Details
    ISSN: 0192-8651
    Keywords: Computational Chemistry and Molecular Modeling ; Biochemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Computer Science
    Notes: We studied geometries and energies of complexes between water and neutral or protonated imidazole by ab initio molecular orbital calculations using the 4-31G basis set with and without the counterpoise correction. Positions of hydration sites and relative binding energies could be also estimated by using the electrostatic field map of imidazole as calculated by our bond increment method. The reliability of the calculations is confirmed by comparing the geometries of the imidazole-water complex to the experimental ones from the Cambridge Structural Database. These were obtained by X-ray diffraction studies on crystals with water bound to a molecule containing the imidazole fragment.
    Additional Material: 10 Ill.
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    URL: http://dx.doi.org/10.1002/jcc.540110906
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    Molecular mechanics calculations of several lanthanide complexes: An application of the random incremental pulse search (1990)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Computational Chemistry 11 (1990), S. 1061-1071 
    Details
    ISSN: 0192-8651
    Keywords: Computational Chemistry and Molecular Modeling ; Biochemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Computer Science
    Notes: A new method for the evaluation of metal complexes with molecular mechanics calculations is described. The method has been employed to determine the global minimum energy conformations of three seven-coordinate lanthanide complexes. The method searches the potential energy surface of the metal complexes for the global minimum structure using the recently reported Random Incremental Pulse Search (RIPS). The molecular mechanics calculations were performed using the MM2 metal-extended force field (MM2MX). This force field excludes 1-3 bending terms about the metal center and, instead, explicitly includes 1-3 nonbonded van der Waals interactions. This affords a model based mainly on steric interactions about the ligating atoms, and removes the necessity of specifying ligand-metal-ligand bond angles for seven-coordinate and higher metal complexes in which no preferred bond angle can be proficiently determined. The calculated minimum-energy structures were well known seven-coordinate conformations in all cases, and a comparison with X-ray crystallographic is presented.
    Additional Material: 9 Ill.
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    URL: http://dx.doi.org/10.1002/jcc.540110908
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  • 24
    Electronic Resource
    Electronic Resource
    MRD-CI calculations of proton affinity within the ab initio method for approximation of the frozen molecular fragment (1990)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Computational Chemistry 11 (1990), S. 1076-1079 
    Details
    ISSN: 0192-8651
    Keywords: Computational Chemistry and Molecular Modeling ; Biochemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Computer Science
    Notes: The method of approximation of the frozen molecular fragment (FMF) we derived has been applied to calculations of proton affinities. Results are in good agreement with experimental data and extended basis set calculations.
    Additional Material: 3 Tab.
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    URL: http://dx.doi.org/10.1002/jcc.540110910
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    Molecular mechanics study of myelin basic protein peptide 87-118: Some local energy minima (1990)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Computational Chemistry 11 (1990), S. 1087-1093 
    Details
    ISSN: 0192-8651
    Keywords: Computational Chemistry and Molecular Modeling ; Biochemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Computer Science
    Notes: Myelin basic protein (MBP) is the major extrinsic protein of the myelin sheath in the central nervous system. It is this protein that is destroyed in such demyelinating diseases as multiple sclerosis. We have examined the predicted structures of one segment of MBP using the molecular mechanics program ECEPP83 developed by Scheraga and co-workers as modified by Chuman, Momany, and Schafer. We have focused upon a segment, 87-118, containing the Pro-Pro-Pro sequence (residues 100-102), which has been predicted from standard algorithms to exist in a hairpin loop connecting anti-parallel beta-strands. Several local energy minima have been found and reported. Tripoline sequences are not rare in proteins, but their structure and function is still uncertain.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcc.540110912
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    PRODEN: A new program for calculating integrated projected populations (1990)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Computational Chemistry 11 (1990), S. 1101-1110 
    Details
    ISSN: 0192-8651
    Keywords: Computational Chemistry and Molecular Modeling ; Biochemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Computer Science
    Notes: Two new computational methods for the evaluation and partitioning of projected, planar, and averaged slab electron densities, implemented in the program PRODEN, are presented. The new algorithms for the projection, demarcation, and integration of electron densities are described and evaluated in terms of speed and numerical accuracy. Integrated Projected Populations are analyzed and some of the advantages and limitations of the methods are discussed.
    Additional Material: 4 Ill.
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    URL: http://dx.doi.org/10.1002/jcc.540110914
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    The effect of diffuse functions on minimal basis set superposition errors for H-bonded dimers (1990)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Computational Chemistry 11 (1990), S. 930-942 
    Details
    ISSN: 0192-8651
    Keywords: Computational Chemistry and Molecular Modeling ; Biochemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Computer Science
    Notes: The effect of the addition of diffuse functions of sp type on the first row atoms (and/or of d type on phosphorus) to a MINI-1 minimal basis set is evaluated by comparing the SCF description of the interaction energy and its decomposition, counterpoise (CP) corrected and uncorrected for basis set superposition errors (BSSEs), with that produced by extended basis sets (6-31G** and 3-21G+) as well as by basis sets (6-31G** + VPS and (2d)S), previously successfully tested, designed to minimize the BSSE. The systems considered are a few anions (F-, HCOO-, H2PO-4) H-bonded to water; the basis set performance on a different geometry (bifurcated instead of linear) of the phosphate-water adduct was also considered. An additional comparison with the interaction energy in a neutral dimer, (HF)2, was carried out. The addition of diffuse functions to the MINI-1 basis set, instead of resorting to extended basis sets, seems to be a sensible choice for anions. The equilibrium distance is shifted towards the reference basis set value at the CP corrected level as well as at the uncorrected one. The spread of the energy due to the basis set is hardly distinguishable when the CP corrected values are plotted. The description of the interaction energy in dimers involving phosphorus is improved using d type diffuse functions.
    Additional Material: 11 Ill.
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    URL: http://dx.doi.org/10.1002/jcc.540110805
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    Masthead (1990)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Computational Chemistry 11 (1990) 
    Details
    ISSN: 0192-8651
    Keywords: Computational Chemistry and Molecular Modeling ; Biochemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Computer Science
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcc.540110901
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  • 29
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    Theoretical analysis of the electronic spectrum of GeH4 from ab initio CI calculations (1990)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Computational Chemistry 11 (1990), S. 1017-1020 
    Details
    ISSN: 0192-8651
    Keywords: Computational Chemistry and Molecular Modeling ; Biochemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Computer Science
    Notes: Ab initio CI calculations using pseudopotentials to describe germanium inner electrons are carried out on the low-lying excited singlet states (T2) of GeH4. A theoretical analysis of these states in terms of Mulliken population of Rydberg orbitals for each state and oscillator strengths allow us to reinterpret its experimental Vacuum UV electronic spectrum.
    Additional Material: 3 Tab.
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    URL: http://dx.doi.org/10.1002/jcc.540110903
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    GEPOL: An improved description of molecular surfaces. I. Building the spherical surface set (1990)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Computational Chemistry 11 (1990), S. 1047-1060 
    Details
    ISSN: 0192-8651
    Keywords: Computational Chemistry and Molecular Modeling ; Biochemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Computer Science
    Notes: The algorithm used by the program GEPOL to compute the Molecular Surface (MS), as defined by Richards, is presented in detail. GEPOL starts like other algorithms from a set of spheres with van der Waals radii, centered on the atoms or group of atoms of the molecule. GEPOL computes the MS by first searching the spaces inaccessible to the solvent and consequently filling them with a new set of spheres. Here we study the behavior of the method with its parameters, presenting several examples of application.
    Additional Material: 13 Ill.
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    URL: http://dx.doi.org/10.1002/jcc.540110907
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    A boundary element method for molecular electrostatics with electrolyte effects (1990)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Computational Chemistry 11 (1990), S. 1080-1086 
    Details
    ISSN: 0192-8651
    Keywords: Computational Chemistry and Molecular Modeling ; Biochemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Computer Science
    Notes: A boundary element method is developed to compute the electrostatic potential inside and around molecules in an electrolyte solution. A set of boundary integral equations are derived based on the integral formulations of the Poisson equation and the linearized Poisson-Boltzmann equation. The boundary integral equations are then solved numerically after discretizing the molecular surface into a number of flat triangular elements. The method is applied to a spherical molecule for which analytical solutions are available. Use is made of both constant and linearly varying unknowns over the boundary elements, and the method is tested for various values of parameters such as the dielectric constant of the molecule, ionic strength, and the location of the interior point charge. The use of the boundary integral method incorporating the nonlinear Poisson-Boltzmann equation is also briefly discussed.
    Additional Material: 6 Ill.
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    URL: http://dx.doi.org/10.1002/jcc.540110911
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    Untersuchungen zur Konstruktion von Bordetella bronchiseptica-Mutantenstämmen durch Transposonmutagenese und deren Prüfung zur Eignung als potentielle Impfstämme (1990)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990), S. 185-191 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Three wild type strains of B. bronchiseptica were mutagenized with the transposon Tn 5. Since the transponson Tn 5 encodes resistance to kanamycin, a selection for resistance to these antibiotics can be used as an indicator for Tn 5 transposition.9 mutants were identified that are not able to grow on minimal medium. Subsequent auxotrophic tests on these mutants revealed 7 valine, 1 cysteine and 1 valine and leucine auxotrophic mutants.It was not possible for us to find B. bronchiseptica transconjugants in the virulent phase I. All in vivo and in vitro tested parameters showed that the isolated mutants were avirulent in phase III.
    Additional Material: 1 Ill.
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    URL: http://dx.doi.org/10.1002/abio.370100217
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    Osnowyi Obschei Mikologii. Kiew: Golownoe Isdatelstwo Isdatelskogo Objedinenija „Vischn Schkola“, 1989 (in Russisch). 590 S., Rbl. 1,40, ISBN 5-11-001357-8 (1990)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990), S. 192-192 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370100219
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    Bioproducts and bioprocesses. Second Conference to Promote Japan/U.S. Joint Projects and Cooperation in Biotechnology, Lake Biwa, Japan, September 27-30, 1986. Berlin, Heidelberg, New York, London, Paris, Tokyo: Springer-Verlag, 1989., 377 pp., 182 figs., DM 198,-, ISBN 3-540-50461-3 (1990)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990), S. 191-191 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370100218
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    DECHEMA - Monographien. Vol. 113. Steriles Arbeiten in der Biotechnik. Vorträge vom Dechema-Symposium vom 7.-9. Dezember 1987. Weinheim: VCH vertragsgesellschaft, 1987. 288 S. (1990)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990), S. 202-202 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370100221
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  • 36
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    Untersuchungen zur in vitro- und in vivo-Adhärenz von Bordetella bronchiseptica (1990)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990), S. 193-201 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Pathogenic strains of Bordetella bronchiseptica undergo phase changing under in vitro conditions. This is the irreversible loss of virulence factors. Adhesins are involved in these structures. They permit adhesion and colonization of bacteria to respiratory epitheliums. Phase III-bacteria adhere as well as Phase I-bacteria under in vitro conditions (cells of respiratory epithelium from guinea pigs).Four phase III-strains of seven isogenic pairs adhere in a significant higher number per cell than phase I-agents. These results are confirmed by results of investigations with phase III-strains constructed throughout cultivation on several culture media. Phase III-bacteria colonize in significant lower numbers under in vivo conditions in rabbits and guinea pigs. Purin auxotrophic mutant strains colonize less in guinea pigs than in rabbits. In vitro adhesion does not reflect the real bacteria-host interaction between adhesin and receptor.
    Additional Material: 5 Ill.
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    URL: http://dx.doi.org/10.1002/abio.370100220
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    Masthead (1990)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990) 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370100301
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    Growth and dinitrogen fixation by azospirillum, species grown under various growth conditions (1990)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990), S. 329-336 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The effect of strain specificity, various culturing conditions, and incubation periods on the growth and efficiency of dinitrogen fixation by A. lipoferum and A. brasilense, grown in semisolid N2 malate medium was investigated.The properties of the particular strain markedly affected the proliferation as well as the nitrogen gains of Azospirillum in either growth condition. In A. lipoferum strain 1244 and A. brasilense strrain 1771 the multiplication rates were much higher, the counts reaching 7 × 106 and 9 × 106 cells/ml after 15 days in case of 50/300 ml growth condition, associated with high nitrogen gains reaching 99.8 and 114.3 mgl-1, while in A. lipoferum strain 8670 and A. brasilense strain 5677 the counts were 6 × 105 and 2 × 106 cells/ml after both the same period and growth condition, associated with low nitrogen gains, reaching 62.5 and 69.6 mgl-1, i. e., the amounts of nitrogen fixed is usually linked to cell proliferation. The microbial activity in both nitrogen fixation and oxidation of carbonaceous material is directly reflected on the efficiency of dinitrogen fixation. In all treatments the efficiency of N2-fixation generally increased markedly with time. In A. lipoferum strains 1244 and 8670 the efficiency of N2-fixation was 118.5 and 107.4 in case of 50/100 ml growth condition after 20 days, while in A. brasilense strains 1771 and 5677 the corresponding figures were 126.5 and 115.4. The response of the available oxygen was linked to the growth conditions, accordingly, the efficiency of N2-fixation increased by 4.4% & 8.8% in case of A. lipoferum strains 1244 and 8670, and 11.0% & 9.8% in case of A. brasilense strains 1771 and 5677 as a result of increasing the surface area: depth ratio by altering the volume of medium in the experimental flasks.
    Additional Material: 1 Ill.
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    URL: http://dx.doi.org/10.1002/abio.370100404
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    Effect of organic growth factors upon the biomass production of some yeasts (1990)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990), S. 349-353 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Some asporgenous yeasts, isolated from various fruits have been examined for their organic growth factor requisites. Of the fifteen growth factors tested, thiamine, biotin, pyridoxine and inositol are the most commonly required organic growth factors by the yeasts, whereas rest of the growth factors showed variable essentiality. All the species of yeasts investigated required different concentrations of growth factors for their optimum growth. Concentration higher than the optimum have been found to be inhibitory for the growth of all the yeasts studied.
    Additional Material: 1 Ill.
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    URL: http://dx.doi.org/10.1002/abio.370100407
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    Cassava whey treatment with aspergillus niger (1990)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990), S. 341-348 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Treatment of Cassava whey with Aspergillus niger was investigated. Heated cassava whey supplemented with ammonium sulphate (NH4)2SO4 and potassium di-hydrogen phosphate (KH2PO4) called supplemented cassava whey (SUCW); heated but unsupplemented cassava whey (USCW); and cassava whey as is, unsupplemented and unheated (UHCW), were used as waste sources and growth media. Total reducing sugars, starch, total carbohydrate, specific gravity, hydrocyanic acid (HCN), biochemical oxygen demand (BOD) and chemical oxygen demand (COD) of A. niger inoculated samples and uninoculated set were monitored on a time course basis for 5 days using standard methods. Percentage reductions in the BOD and COD were 69.7 and 54.5 respectively for SUCW samples; 42.4 and 29.5 for USCW samples while values for UHCW were 9.0 and 4.5. The reductions in reducing sugars, starch, total carbohydrate, specific gravity and cyanide content in descending order were SUCW; USCW and UHCW. The heated and A. niger inoculated cassava whey samples were better purified than both the unheated but inoculated and unheated and uninoculated cassava whey samples.
    Additional Material: 2 Ill.
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    URL: http://dx.doi.org/10.1002/abio.370100406
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    Die europäische Patentanmeldung und der PCT in Frage und Antwort. Köln, Berlin, Bonn, München: Carl Heymanns Verlag KG, 1989, 2nd edition 263 pages; DM 68,- ISBN 3-452-21 286-6 (1990)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990), S. 354-354 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370100408
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    Microbial enzymes and biotechnology. Barking, Essex: Elsevier Science Publishers Ltd., 1983, 382 S., 73 Tab., 29 Fig., Ł 49.00 ISBN 0-85334-185-0 (1990)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990), S. 360-360 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370100410
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  • 43
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    Purification of human tissue-type plasminogen activator (t-PA) from melanoma cell culture medium by different affinity chromatographic methods (1990)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990), S. 355-360 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Supernatants of a melanoma cell line were chromatographed on five affinity supports to evaluate and compare purification protocols for isolation of tissue-type plasminogen activator (t-Pa).t-PA was effectively adsorbed quantitatively from cell culture supernatants by lysine-Sepharose, concanavalin A-Sepharose, zinc-chelate-Sepharose and monoclonal antibody-Sepharose. The major part of t-PA was effectively adsorbed also by heparin-Sepharose. About 20 per cent of t-PA activity present in the culture medium however was not bound to the latter support, which indicates a heterogeneity of melanoma t-PA. The heterogeneity is not due to carbohydrate variants I and II since both vriants were adsorbed and eluted from all the affinity supports investigated.Highly enriched t-PA preparations were obtained by sequential chromatography on two supports. t-PA isolated by monoclonal antibody affinity chromatography and subsequently purified by gel filtration was of highest purity. Other useful combinations were chromatography on concanavalin A-Sepharose or lysine-Sepharose followed by chromatography on heparin-Sepharose, or zinc-chelate-Sepharose followed by chromatography on lysine-Sepharose.
    Additional Material: 1 Tab.
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    URL: http://dx.doi.org/10.1002/abio.370100409
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    Purification and partial characterization of external and internal invertases from two strains of trichosporon adeninovorans (1990)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990), S. 361-370 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: We investigated the extracellular and intracellular invertase activity of two Trichosporon adeninovorans strains. Both strains (SBUG 724 and CBS 2844) secrete one invertase into the medium. The external enzymes were purified by chromatography on hydroxylapatite. The molecular weight of the external invertase of SBUG 724 was found to be 650,000 and of CBS 2844 450,000. The internal invertases were separated by DEAE-cellulose chromatography. The molecular weight of the enzyme from CBS 2844 was estimated to be 125,000. In the strain SBUG 724 we found two internal invertases (Mr 230,000 and 70,000). The activity of all invertases has similar properties. The pH optimum of the reaction was determined between 5.0 and 5.2 and the temperature optimum was 60 to 70°C. The Km value for sucrose was determined to be 71 to 83 mM and for raffinosc 27 to 36 mM.
    Additional Material: 7 Ill.
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    URL: http://dx.doi.org/10.1002/abio.370100411
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  • 45
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    Intensification of glucose oxidase synthesis with aspergillus niger by the way of multistage mutagenization (1990)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990), S. 371-376 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Conidia of glucose oxidase active Aspergillus niger G 13 strain were subjected to the fourth-stage mutagenization using different combinations of mutagens in each stage (stage I - UV radiation + nitrosomethylurea, stage II - UV radiation + ethyleneimine, Stage III - acryflavine, stage IV - UV radiation + N-methyl-N′-nitrosoguanidine). In all, 400 strains which showed higher glucose oxidase activity with our own diffusion data method (were isolated out of 5208 colonies after mutagenization). Nine most active mutants were tested in the submerged culture determining the activity of glucose oxidase in the post-culture liquid which increased from 10.7 to nearly 30%.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370100412
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    Masthead (1990)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990) 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370100501
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  • 47
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    Production of active mutant strains of penicillium canescens 20171, forming complex preparation of exogenous glycosidases (1990)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990), S. 377-381 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The ability to synthesize extracellular β-galactosidase (EC 3.2.1.23), α-galactosidase (EC 3.2.1.22), β-fructofuranosidase (EC 3.2.1.26) in Penicillium canescens mutant strains (300 cultures) after UV-irradiation has been checked. Most of the investigated mutants had the ability to produce the mentioned enzymes in an increased amount in comparison with initial strain. The ability of extracellular enzyme biosynthesis in P. canescens mutant strains was increased for α-galactosidase on 200%, for β-galactosidase on 275% and for β-fructofuranosidase-220%. The mutant strains were stable during three years of observation, complitely maintaining increased activity.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370100413
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  • 48
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    Electronic Resource
    Dihydrodipicolinate synthase of lysine excreting and non excreting strains of bacillus megaterium (1990)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990), S. 382-384 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Dihydrodipicolinate synthase activity of lysine producing strain of Bacillus megaterium and its high yielding mutant AECr 60f was highest during the end of the exponential phase and remained at the same level during the stationary phase when major bulk of lysine was accumulated. In comparision in a lysine non producing strain of B. megaterium the activity of the enzyme was low. Lysine producing strains has DHD synthase with two specialities, persistence during the stationary phase and insensitivity to repression by exogenous lysine. The high yielding mutant has increased DHD synthase activity which was not sensitive to feed back inhibition with lysine.
    Additional Material: 2 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370100414
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  • 49
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    Entwicklung der biotechnologie in der UdSSR. Moskau: Verlag Nauka, 1988, 200 S., 6 Abb. (1990)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990), S. 34-34 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370100109
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  • 50
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    Interbiotech '87, enzyme technologies, progress in biotechnology vol. 4. Amsterdam, Oxford, New York, Tokyo: Elsevier Science Publishers. 1988, S. 535, $ 171.00 Dfl325.00, ISBN 0-444-98 924-2 (1990)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990), S. 40-40 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370100111
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  • 51
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    Adhesins of escherichia coli (1990)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990), S. 151-161 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: E. coli has got increasing importance as a causative agent of intestinal and extra-intestinal diseases. In both these infections adhesion of the bacteria to mucous surface cells are initial events for coionization and development of infection. Adhesins are bacterial recognition proteins which specifically interact with carbohydrate moieties of glycoproteins or glycolipids on mammalian cells. The adhesiveness of bacteria is associated with filamentous surface appendages, designated as fimbriae or pili, as well as with non-fimbrial components. Some recent data on the nomenclature, classification, disease association, receptor specificity, and topographic arrangement are presented. The correlation between E. coli O : K : H serovar and fimbrial antigens is demonstrated on the basis of E. coli isolated from patients with urinary tract infections. Hitherto unknown non-fimbrial adhesins are briefly described.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370100211
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  • 52
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    Nachweis humoraler und lokaler Antikörper nach Einsatz einer bordetella Bronchiseptica-Lebendvakzine am Schwein (1990)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990), S. 179-184 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The aim of this work was to prove if a Bordetella bronchiseptica living vaccine is able to induce humoral and local antibodies in pigs. The living vaccine was compared with a killed Bordetella bronchiseptica adjuvans vaccine. The results show that piglets of sows vaccinated parenterally with living vaccine and killed adjuvans vaccine develop a stronger humoral immune reaction than piglets vaccinated locally with living vaccine. Sows immunized parenterally with living vaccine permint their piglets in the first three weeks colostral antibodies like sows immunized with killed adjuvans vaccine. The living vaccine induces especially local antibodies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370100216
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  • 53
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    VCH forschungsführer. Chemie und biochemie in der bundesrepublik deutschland. Weinheim, Basel, Cambridge, New York: VCH Verlagsgesellschaft, 1989. 673 pp., DM 196,-, ISBN 3-527-26809-x (1990)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990), S. 178-178 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370100215
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  • 54
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    Comparison of mitotic activity and growth in two long term callus cultures of matricaria recutita L. (1990)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990), S. 245-251 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Callus cultures of two tetraploid plant of Matricaria recutita L. were initiated from leaves and cultured on LINSMAIER-SKOOG culture medium supplemented with 0.01 mg · 1-1 IAA or 3.00 mg · 1-1 2, 4-D for three years. The relationship between mitotic activity and growth of calli was studied. The time at which mitotic activity began in both cultures was different. In the callus culture cultivated on the medium with IAA the maximum mitotic activity was observed on the 14th to 17th day of subculture. In the one cultivated on the medium with 2,4-D, the increase of mitotic activity was observed earlier and corresponded with the beginning of exponential growth. In addition of total mitotic activity individual mitotic phases have also been evaluated. Most mitotically active cells in both cultures were in prophase. Their courses were similar to those of total mitotic activity. Metaphase and telophase stages were present in low frequency. Anaphase occured rarely. The functional dependences of mitotic activity and growth were characterized by appropriate mathematical models.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370100306
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  • 55
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    Patentgesetz. 4th revised and enlarged edition Köln, Berlin, Bonn, München: Carl Heymanns Verlag KG, 1987. 987 p., DM 140,-, ISBN 3-452-20944-x (1990)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990), S. 540-540 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370100614
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  • 56
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    Characteristics of the immobilized pectin lyase activity from a commercial pectolytic enzyme preparation (1990)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990), S. 531-539 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: A commercial pectolytic enzyme preparation has been immobilized onto a nylon-polyethyleneimine copolymer, in order ot study the contribution of the pectin lyase activity inthe overall pectindegrading activitg. Optimal conditions for the immobilization process of the pectin lyase activity, such as chemical modification of the support, coupling pH and protein concentration, were determined.Kinetic parameters and temperature behaviour of both the soluble and immobilzied pectin lyase activitgy of the derivative were also determined. OPerational stability of the pectin lyase and overall viscosity reducring activities resulting in a halflife times of 3.8 and 8.5 days, respectively, for both pectolytic activities, when the immobilized derivatives were tested in a cross-flow reactor, using a highly esterified pedtin as substrate.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370100613
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  • 57
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    Genetics of bacterial diversity. London, New York, Tokyo: Academic Press, 1989. 449 pp., Ł 14.95, ISBN 0-12-355574-4 (1990)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990), S. 550-550 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370100616
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  • 58
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    Investigation of the connection between the electrophoretic mobility (EPM) of microorganisms and their capability of metal uptake (1990)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990), S. 541-549 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The electrophoretic mobility of microorganisms (EPM) as a measure of their electric surface charge was determined as a function of different milieu conditions with the aid of the “Parmoquant 2” cell electrophoresis apparatus manufactured by CARL ZEISS Jena.The object of these researches was to examine the influence of the electric surface charge of microorganisms on their metal loading capacity. The results show a direct correlation between the electric surface charge or the EPM of microorganisms and their maximum metal loading capacity. Cells with a high negative surface also posses a high metal binding capacity. On the other side only a negligible metal uptake can be observed at the isoelectric point of the microorganisms (EPM = 0). The method of cell electrophoresis proved suitable to analyze complex interactions between microorganisms and heavy metal ions.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370100615
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  • 59
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    Separations for biotechnology. Chichester: Ellis Horwood Ltd. 1987, 502 S., 179 Abb., Ł 55.00 (1990)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990), S. 560-560 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370100618
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  • 60
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    Methods in molecular biology. Vol. 4: New Nucleic Acid Techniques. Clifton, New Jersey: Humana Press, 1988. 560 pp., Ł 42.00, isbn 0-89603-127-6 (1990)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990), S. 564-564 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370100622
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  • 61
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    Biotechnology at the treshold of the XXI century - ‘acta biotechnologica‚ is entering its second decennium (1990)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990), S. 3-5 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370100102
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  • 62
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    Progress in industrial microbiology. Vol. 25. Computers in fermentation technology. Amsterdam, Oxford: Elsevier, 1988. 198 pp., $ 73.75, Dfl. 140.00, ISBN 0-444-429794 (1990)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990), S. 6-6 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370100103
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  • 63
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    Genetics of Translation. New approaches. NATO ASI Series H: Cell Biology, Vol. 14. Berlin, Heidelberg, New York: Springer-Verlag, 1988. 524 S., 145 Abb., Dh 248,-ISBN 3.540.18550-X (1990)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990), S. 12-12 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370100105
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  • 64
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    Bioprocesses including animal cell culture. Berlin: Akademie-Verlag, DDR, 1988. 168 S., 41 Fig., 12 Tab., M. 148,-, ISBN 3-05-500569-4 (1990)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990), S. 22-22 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370100107
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  • 65
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    The principle of optimality as the foundation of biotechnological modeling (1990)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990), S. 23-34 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: There are many points of contact between optimization problems and modeling. On the one hand the model adjustment process itself as a process of estimation is closely connected with optimization, in that it is to produce what is in one sense the best possible model. The basic structure of the optimization problem as problem in decision making with the necessary input of an objective function is thus evident. On the other hand a model is never an end in itself but on the basis of its simulation capacity a means to an end, for example in biotechnological optimization. From this point of view the model is a product of scientific work and thus an economic value. Equally, through its intended purpose the model exhibits a utility value. A complete evaluation of the model as a condition of rational modeling must take into account both these aspects. That is possible in principle by adding the modeling expenditure to expenditure for the realization of biotechnological processes, expressing the economic consequences of model quality as an objective function, and minimizing the specific total expenditure for the product to be produced.Biotechnological practice requires that the “optimum” model is approached by means of iterative processes. Some practical examples will make the process clear, taking into account qualitative (semantic) and quantitative (accuracy) aspects of the utility value.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370100108
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  • 66
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    Microbial proteinases. The amidase from a brevibacterium strain: Study and applications. Advances in biochemical engineering/biotechnology. Vol. 36 Enzyme studies. Berlin: Akademie-Verlag, 1988. 115 S., M 108,-, ISBN 3-05-500568-6 (1990)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990), S. 48-48 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370100113
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  • 67
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    Antibiotic resistance genes: Ecology, transfer, and expression. Banbury Report 24. Cold Spring Harbor: Cold Spring Harbor Laboratory, 1987, S 68,00, XIV, 436 pp. ISBN 0-87969-224-3 (1990)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990), S. 54-54 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370100115
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  • 68
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    Fructose phosphorylation by zymomonas mobilis glucokinase (1990)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990), S. 49-53 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Zymomonas mobilis ATCC 53431, a fructokinase negative mutant is unable to utilize fructose as a sole carbon source for growth. At high fructose concentrations, however, fructose was converted to ethanol. The fructose uptake displayed MICHAELIS-MENTEN kinetics with an apparent Km of 185 mM fructose.Purified glucokinase from ATCC 53431 and the wild strain ATCC 29191 both exhibited fructose phosphorylating activity at high fructose concentrations with an apparent Km value of 222 mM fructose. Glucokinase substrate specificity was found not be absolute, as previously reported.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370100114
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  • 69
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    Isolation of auxotrophic mutants and development of conjugation system in KISR methylotrophs (1990)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990), S. 93-98 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Isolation of auxotrophic mutants and conjugal transfer of plasmids are successfully demonstrated in four obligate methylotrophic strains isolated at Kuwait Institute for Scientific Research (KISR)Out of 11 auxotrophic, mutants, three required methionine and two leucine. Complete transfer of plasmid R68.45 from Pseudomonasto KISR methylotrophs and vice versa have been achieved with a low transfer rate.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370100123
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  • 70
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    The microbiological data base of the data bank project BD1 (1990)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990), S. 79-84 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The aim of the project BD1 (Biotechnology Data Bank 1) is to improve the data processing for research in biotechnology. In BD1, data of several classes of objects and methods are compiled. A data file conception consisting of object data files, method data files and feature data files was created, which can be easily extended to further classes, and which allows the combination of different classes of features to the same class of objects. There are two main approaches to use BD1: 1. making available informations on certain biotechnological objects, 2. the identification of unknown objects by means of their feature pattern.The microbiological data base and the possibilities of data base search are presented by example of lactic acid bacteria.
    Additional Material: 7 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370100121
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  • 71
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    Nonionic tensides modify papain structure and proteolytic activity (1990)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990), S. 85-92 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The effect of 33 polyethoxylated nonionic tensides with various hydrophobic moieties was studied on the proteolytic activity and phase transition behaviour of papain. Tensides with longer ethyleneoxide chain markedly increased the phase transition temperatures of papain indicating possible hydrogen bond formation between the hydrophilic ethyleneoxide chain and the polar substructures of papain. The character of lipophilic moiety of tensides influences also each physicochemical parameter of papain suggesting the existence of hydrophobic interactions too. The significant positive linear correlations between the activity increasing and structure stabilizing effects of tensides make probably that the structure modification of papain accounts for its enchanced proteolytic activity.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370100122
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  • 72
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    Heterotrophic eubacteria isolated from cultures of the cyanobacterium, spirulina maxima (1990)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990), S. 99-104 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Three distinct heterotrophic eubacterial strains were isolated from mixotrophic cultures of the filamentous cyanobacterium, Spirulina maxima (Gom) Geitl. Spirulina spp. are considered to be prime candidates for the phototrophic production of biomass protein, particularly in developing countries. These cyanobacteria are extreme alkaliphiles and halophiles, making their production in arid regions promising.Most previous studies on the eubacteria which live in Spirulina culture systems have focused on determining the possible presence of pathogenic species in biomass protein. Little has been done to understand the symbiotic relationships between the cyanobacterium and its eubacterial cosymbionts. From the perspective of a heterotrophic eubacterium, autotrophic cultural systems of Spirulina have limited carbon and energy resources, being limited to cyanobacterial exudates.In this study, three eubacterial strains were isolated and studied. One strain, a Gram-negative, non-sporing, motile rod, grew exceptionally well in a mineral salts medium where only a small amount of a single low molecular weight organic compound (e.g., acetate) was supplied as sole energy source. This strain was also extremely euryresponsive with respect to salinity and alkalinity as well. Two less well-adapted eubacterial strains are also described.
    Additional Material: 2 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370100124
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    Masthead (1990)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990) 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370100201
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  • 74
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    Plant cell biotechnology. (NATO ASI series H: Cell Biology, Vol. 18). Berlin, Heidelberg, New York, London, Paris, Tokyo: Springer-Verlag, 1988 500 pp., 92 figs., DM 298.00, ISBN 3-540-18 556-9 (1990)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990), S. 104-104 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: NO ABSTRACT.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370100125
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  • 75
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    Safety assurance for environmental introductions of genetically-engineered organisms. (NATO AS1 series G: Ecological sciences, Vol. 18). Berlin, Heidelberg, New York, London, Paris, Tokyo: Springer-Verlag, 1988. 282 pp., DM 148,-, ISBN 3-540-18561-5 (1990)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990), S. 116-116 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370100203
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  • 76
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    Techniques in cell cycle analysis. Clifton, New Jersey: Humana Press Inc, 1987. 407 pp., S 79.50, ISBN 0-89603-097-0 (1990)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990), S. 124-124 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370100205
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  • 77
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    Significance of composition and structure of the enterobacterial outer membrane to the transport of desired and undesired substances and its inhibition (1990)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990), S. 107-115 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The enterobacterial outer membrane forms a bilayer. Its outer monolyer consists of lipopolysaccharides and proteins, its inner monolayer of phospholipids and proteins. It thus represents an efficient penetration barrier against hydrophobic and anionic compounds (such as detergents or hydrophobic antibiotics) and against higher molecular substances (such as proteolytic, lipolytic, and murolylic enzymes).Some of the proteins (“porins”) form channels through the outer membrane through which neutral and cationic hydrophylic compounds up to a molecular weight of about 800 can pass. Besides the porins additional transport systems have been described. They play an important part in providing the bacteria with substances necessary for their growth, i.e., phosphate, iron ions, and others.Organic polycations are able to generate more or less severe disorganizations in the outer membrane through which they can pass the bilayer (“self-promoted pathway”). Some of these polycations represent efficient antibiotics (polymyxin B, nourseothricin). Bacteria are able to protect themselves against the harmful action of these substances by changing the composition of the outer membrane.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370100202
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  • 78
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    Outer membrane vesiculation of acinetobacter calcoaceticus (1990)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990), S. 117-123 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: For intact cells of A. calcoaceticus 69V susceptibility to hydrophobic agents (antibiotics, dyes) was established. The composition of its outer membrane and comparison with that of a reference strain, A. calcoaceticus CCM 5593 with a blocked hydrophobic pathway, gave no indication of phospholipid bilayer domains as the structural basis of these permeability characteristics. The outer membrane composition together with the data of time-resolved fluorescence anisotropy measurements is indicative of a high state of order of the hydrocarbon region.A. calcoaceticus 69V releases lipopolysaccharide (LPS)-rich membrane vesicles into the growth medium when grown on a hydrophobic carbon source. While the cells contain both R-form and S-form LPS, the LPS released with the vesicles is exclusively of the R-type. The same selectivity with respect to LPS composition was observed when LPS was removed from intact cells by EDTA-NaCl treatment which leads to a break-down of the barrier to hydrophobic agents in A. calcoaceticus CCM 5593.We propose that due to its physical properties, R-form LPS forms tightly packed structures within the membrane which, under certain conditions, become destabilized and liberated into the surrounding medium. As a consequence, a disturbance of the highly ordered lateral molecular arrangement might lead to altered permeability properties of the outer membrane as suggested in one of the two alternative models existing to explain permeability changes observed in deep rough mutant strains of Enterobacteriaceae.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370100204
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  • 79
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    Yeast genetics. A manual of methods. Berlin, Heidelberg, New York, London, Paris, Tokyo: Springer-Verlag, 1989. 104 pp., 10 fig., DM 59,-, ISBN 3-540-18805-3 (1990)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990), S. 142-142 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370100208
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  • 80
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    Structure and serology of the lipopolysaccharides: Cell components of citrobacter (1990)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990), S. 133-141 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The genus Citrobacter consists of enteric rods that utilize citrate as a sole carbon source. Citrobacter serotypes are normal intestinal inhabitants, but some of them have been reported in gastroenteritis and in extraintestinal infections.Our immumochemical studies on Citrobacter lipopolysaccharides of chemotype G comprised serotypes: 04, 027 and 036. As we found the serotypes mentioned above are distinguished in having O-specific polysaccharides that are homopolymers of β [1-2] linked 4-deoxy-D-arabinohexopyranose. In accordance with the structural indentity, the Citrobacter lipopolysaccharides showed strong serological cross-reactivity. Nevertheless, serotypes 04, 027, and 036 form separate entities, as observed in early studies of serologists. To find the reason for the immunological diversity the structural studies on lipopolysaccharide core regions of these Citrobacter serotypes were carried out. Three novel enterobacterial cores were identified. The complete cores 04 and 036 are decasaccharides differing in one structural element only: side-chain glucose is present in 04 oligosaccharide in the place of galactose in the 036. The core 027 is octasaccharide.In conclusion, our structural studies have shown hitherto that in Citrobacter chemotype G group only one O-specific polysaccharide, but three core regions, exist.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370100207
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  • 81
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    Identification of D-galactose-(1 → 7)-3-deoxy-d-manno-2-octulo-pyranosonate as component of the inner core region of escherichia coli EH 100 lipopolysaccharide (1990)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990), S. 163-168 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The dissaccharide D-galactosyl-3-deoxy-D-manno-2-octulosonic acid (Gal-KDO) from lipopolysaccharides (LPS) of the inner core region of the rough mutant EH100 of E. coli 0100 was isolated after mild acid hydrolysis of LPS by means of dialysis, ion-exchange chromatography, gelfiltration and high-voltage paper electrophresis. By chemical analysis galactose and KDO were identified in a molar ratio of approximately 1 : 1. 13C-NMR and 1H-NMR studies, methylation analysis and GLC of the acetylated R.( - )-2-butyl-galactoside identified the structure .
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370100213
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  • 82
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    Some aspects of the regulation of nitrogen metabolism in the yeast genus candida. Study of the kineties of ammonium transport to the cells of hydrocarbon oxidizing yeast of the genus candida with a change in nitrogen nutrition (1990)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990), S. 169-177 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: In his study the kinetics of the ammonium transport to he hydrocarbon-oxidizing yeast cells and the specific features of its regulation as one of the aspects of catabolite nitrogen repression were investigated. As the objects 3 mesophilic strains of C. maltosa, the thermotolerant strain C. rugosa as well as a collection of the thermotolerant strain C. blankii BKM-Y-462 type were selected.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370100214
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  • 83
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    Effect of ensiling on the nutritive value of low quality roughage rice hulls. I. Microbial studies (1990)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990), S. 419-432 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Rice hulls were ensiled in laboratory silos in two separate experiments with the same treatments. In the first, sealing was delayed for a period of days. In the second experiment, silos were sealed immediately after filling. In each experiment, four treatments were applied, namely control, molasses 2% added, urea 0.5% added, and molasses 2% + urea 0.5% added.Lactobacilli, streptococci, and coliforms appear to form the main bulk of the flora characteristic of fresh rice hulls, while the lactic acid bacteria dominate at the commencement of the silage ripening. The characteristics of 30 representative pure strains of both lactic acid and coliform bacteria isolated from rice hulls laboratory silage are described. On the basis of morphological, cultural, and biochemical characteristics, the isolated cultures were identified as ten strains of Lactobacillus ten strains of Streptococcus, five strains of Klebsiella, and five strains of Escherichia.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370100508
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  • 84
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    Römpp Chemie Lexikon (9th edition). Stuttgart, New York: Georg Thieme Verlag, 1989. 6 Volumes (1989-1992), 40,000 entries, DM 1188,- (subscription rate) Vol. 1 (A-Cl). 762 pp., DM 198,-, ISBN 3-13-734609-6 (1990)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990), S. 444-444 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370100511
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  • 85
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    Continuous culture models with intermediates (1990)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990), S. 433-443 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The number of stability of steady states of minimal structured models are determined theoretically. Regulation of two “bottle-necks” of cell metabilism by extracellular or intracellular or intracellular intermediates is suggested. The mechanisms of this regulation include substrate and product feed-forward inhibition as well as feedback product control. The most complex system behaviour is obtained in models with feed-forward intermediate inhibition. In particular these models exhibit multistability and two limit cycles.The maximum number of steady states decreases from five to four when the extracellular intermediate is changed by the intracellular one. The number of non-zero steady states is changed from four to two.The relevant features of phase-portrait appear to be independent of the choice of reaction rates. Therefore our results are suitable for a large group of the third order modles. The possibility to verify the theoretical predictions experimentally is also discussed.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370100510
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  • 86
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    Progress in clinical biochemistry and medicine, Volume 5. Berlin: Akademie-Verlag, 1988. 240 pp., 59 fig., DM 128,-, ISBN 3-05-500395-0 (1990)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990), S. 450-450 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370100513
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  • 87
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    Influence of acid phosphatase activity on the saccharification of potato maltodextrins by aspergillus niger glucoamylase (1990)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990), S. 445-449 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: A preparation of Aspergillus niger acid phosphatase, which had the temperature optimum 60°C, pH optimum 1.8-3.0; good stability at pH 4-5, the ability to hydrolyze glucose-6-phosphate at a high rate, and substantial lack of glucogenic activities, was used simultaneously with a glucoamylase in order to learn its influence on the saccharification of potato maltodextrins.The addition of the acid phosphatase activity in amounts that gave the 50 fold increase, as compared to phosphatase activity which naturally occurs in the glucoamylase (GA) preparation ‘AMG-200’, was found to influence on the DE level, mainly at the high substrate concentration (40% d.s.) and low glucoamylase dosage (60-100 GAU/kg d.s.). It may also be possible, when using the acid phosphatase addition, to shorten the saccharification time.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370100512
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  • 88
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    Classification of yeasts by means of the BD1 data bank (1990)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990), S. 401-410 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Computerized procedures for the classification and discrimination of yeasts are presented which base on feature data files and classification techniques involved in a data bank project (BD1). Microbiological (physiological and morphological) and analytical feature data classes (fatty acid patterns from gas chromatography, fourier coefficeints from FTIR spectra, fluorescence patterns) were used to show the possibilities of yeast classification, carried out by means of these features and statistical or non-statistical methods. Results obtained by a suitable combination of these methods are presented.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370100505
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  • 89
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    Influence of cytochrome composition on the energy conservation of acinetobacter calcoaceticus 69-V (1990)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990), S. 551-560 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Acinetobacter calcoaceticus 69-V contained cytochromes b, o, d and traces of cytochrome a1 after growth on ethanol. Cytochromes o, d, and a1 are known to be potentially capable of functioning as terminal oxidases. After growth on acetate only smaller amounts of cytochromes b and o were detectable. Cytochrome spectra of acetate plus glucose-grown cells resembled more those of ethanolgrown cells. Cytochrome spectra of acetate plus ethanol-grown cells were more similar to those of acetate-grown cells but already showed small amounts of cytochrome d.Ethanol-grown cells were energized only by the oxidation of glucose. But the velocity of ATP synthesis was so low that the P/O quotient amounted to practically zero. The stoichiometries of ATP synthesis of the otherwise-grown cells were generally higher for the oxidation of glucose than for the oxidations of succinate and D-lactate. Similar high P/O quotients were measured for the oxidations of glucose and ethanol in acetate plus ethanol-grown cells.No significant energization could be observed during the oxidations of gluconate and acetate.The findings indicate that electrons delivered by the PQQ-dependent glucose as well as by NAD+- dependent ethanol oxidations have probably a common place of entry into the respiratory chain. Glucose and NADH appeared to be energetically equivalent although at least two energetically different pathways might be involved in substrate oxidation.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370100617
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  • 90
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    Römpp Chemie Lexikon (9th edition). Stuttgart, New York: Georg Thieme Verlag, 1990. 6 Volumes (1989-1992), 40,000 entries, DM 1188.- (subscription rate) Vol. 2 (Cm-G), pp. 763-1678, DM 198,-, ISBN 3-13-734-709-02 (1990)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990), S. 561-561 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370100619
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  • 91
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    Symmetry through the eyes of a chemist. New York: VCH Publishers, Inc. 458 pp., 380 photographs, figures and drawings (1990)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990), S. 62-62 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370100117
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  • 92
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    FERM - a decision support system for fermentation processes (1990)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990), S. 63-71 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: For recombinant DNA product fermentations in the laboratory and pilot scale as well as for antibiotic fermentations at the industrial scale decision support program systems for personal computers were developed. It assists the operator to solve control and decision tasks. Applying this program the data and knowledge bases increase, which include measured and derived process data, parameters and comments of previous fermentation runs as well as algorithmic and declarative rules.
    Additional Material: 3 Ill.
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    URL: http://dx.doi.org/10.1002/abio.370100118
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  • 93
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    Patents: A basic guide to patenting in biotechnology. (Cambridge Studies in Biotechnology: 6). Cambridge: Cambridge University Press, 1988. 191 S., $ 49.50, ISBN 0521-32954-X (1990)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990), S. 72-72 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370100119
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  • 94
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    Ethanolic fermentation with saccharomyces cerevisiae cells immobilized in pectate gel (1990)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990), S. 55-61 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: High-molecular weight pectic acid with a STAUDINGER index of 210 ml/g and a degree of esterification of 3%was used as matrix material for the immobilization of Saccharomyces cerevisiae cells. In discontinuous and continuous fermentation tests the gel beads obtained exhibited the same biomass loading capacity (152-155 g dry wt. cells/kg gel) and about the same maximum specific productivity (103.0 g ethanol/kg gel · h) as alginate immobilizates. But there were distinct differences in the swelling behaviour of the two gels. Under the same experimental conditions the increase of bead volume amounted to 27% only for pectate gel in comparison to 129% for alginate gel. In continuous fermentation experiments performed in a horizontal-column packed-bed reactor with liquid recycling a mean steady-state ethanol concetration of 69.1 g/l and a mean productivity of 24.7 g ethanol/lh could be kept constant over a period of more than 10 days.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370100116
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  • 95
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    Effects of temperature on the growth of the thermotolerant methylotrophic bacillus sp. NCIB 12522 in chemostat culture (1990)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990), S. 73-78 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The effects of temperature on chemostat cultures of a thermotolerant, methylotrophic Bacillus ap. are reported. In spite of the relative fastidiousness of this bacterium, overall response to a range of growth temperatures is predictable. However, anomalous results with respect to biomass yield coefficients are evident, suggesting complex and extensive endogenous and exogenous activities under some growth conditions.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370100120
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  • 96
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    Biotechnologie - Lehrbuch der angewandten Mikrobiologie. Dritte, neubearbeitete Auflage. München, Wien: R. Oldenbourg Verlag, 1989 342 S., 224 Abb., 342 Tab., DM 76,-, ISBN 3-486-28403-7 (1990)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990), S. 150-150 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370100210
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  • 97
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    Untersuchungen zur Oberfläuchenstruktur von Pasteurella multocida (1990)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990), S. 143-149 
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    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Detailed studies of the surface structures are an important requirement for the development of efficient vaccines against enzootic pneumonia in calves and piglets caused by Pasteurella multocida.Electron microscopical examination after Alcian Blue staining shows capsular material extending far into the surrounding medium.The extent of the capsules depicted by light microscopy did not correlate with virulence and immunogenicity. However, the extent of the capsules was effected by different culture conditions.The immunization with extract material resulted in a good protection against homologous infection.It has been shown that different cultivation conditions can result in heterogeneity of LPS and in altered OMP-profiles.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370100209
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  • 98
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    Bioorganic marine chemistry, Vol. 2. Berlin, Heidelberg, New York, London, Paris, Tokyo: Springer-Verlag, 1988. 170 pp., 9 fig., 12 tab., DM 118,-, ISBN 3-540-19357-X (1990)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990), S. 162-162 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370100212
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  • 99
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    Electronic Resource
    Brauchbarkeit der ATP- und Dehydrogenaseaktivitätsmessungen, und der Sauerstoffverbrauchsgeschwindigkeit zur Intensitätsbeurteilung des Belebtschlammverfahrens (1990)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990), S. 203-212 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Some measurements of the metabolic activity of microorganisms in a standard activated sludge system have been made. The investigations have shown that the measurements of the respiratory activity of microorganisms are a convenient and reliable method for determining the metabolic activity of microorganisms. The values of respiratory activity have shown a high correlation with the rate of substrate removal in a continuous activated sludge process. Hydrogenase activity has shown moderate correlation with the rate of substrate removal.The ATP values did not correlate with any of the methods of measuring the metabolic activity of microorganisms and the quantity of the dry organic mass of activated sludge. Howeever, the highest correlation between the particular parameters of the activated sludge process was obtained after assuming that the ATP total corresponds to the quantity of the active biomass which fact points at the correctness of such an assumption.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370100222
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  • 100
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    Electronic Resource
    Fungal cell walls: Their structure, biosynthesis and biotechnological aspects (1990)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990), S. 225-238 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The article briefly reviews the present knowledge on various aspects of fungal cell wall structure, function and biosynthesis of its individual components. A special attention is devoted to biotechnological potential of cell walls and cell-wall linked phenomena.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370100303
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