Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
  • 1995-1999  (252)
  • 1935-1939
  • 1890-1899  (20)
  • 1999  (252)
  • 1894  (20)
  • Life and Medical Sciences  (116)
  • gene expression  (79)
  • CT  (77)
Source
Material
Years
  • 1995-1999  (252)
  • 1935-1939
  • 1890-1899  (20)
Year
Keywords
  • 1
    Electronic Resource
    Electronic Resource
    The watershed between right and left hepatic artery territories: findings on CT scans after transcatheter oily chemoembolization of hepatic tumors. A preliminary report (1999)
    Springer
    Surgical and radiologic anatomy 20 (1999), S. 355-360 
    Details
    ISSN: 1279-8517
    Keywords: Liver ; CT ; Neoplasms ; Arteries, therapeutic blockade
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The goals of this study were to delineate the boundary between the right and left hepatic artery territories by computed tomography (CT), to compare this boundary with the middle scissura of Couinaud’s segmental anatomy, and to discuss the clinical implications of these findings. The 18 patients who underwent transcatheter oily chemoembolization (TOCE) of liver tumors via the right or left hepatic artery, were examined with an immediate postembolization CT scan. We measured the orientation of the watershed line between the right and left hepatic artery territories and the orientation of the middle scissura on other available sectional imaging modalities, and then compared the middle scissura with the arterial watershed line. A part of segment IV was fed by the right hepatic artery in two of 18 (11.1%) patients. Moreover, one of 4 segment IV lesions was embolized via right hepatic artery infusion. Thus, in 11.1% of cases there was no coincidence between the arterial watershed line and the middle scissura. Some segment IV lesions may be fed and therefore embolized only via right hepatic artery infusion in TOCE for liver tumor.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s00276-998-0355-0
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Periportal lymphatic distension resulting from cardiac tamponade: CT findings and clinical-pathologic correlation (1999)
    Springer
    Emergency radiology 6 (1999), S. 85-93 
    Details
    ISSN: 1438-1435
    Keywords: Key words Hepatic lymphedema ; CT ; Hemopericardium ; CT ; Aneurysm ; rupture ; Aorta ; CT
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Purpose: To correlate computed tomography (CT) findings with clinical-pathologic results and discuss the possible significance of periportal hypodensity in patients with clinical cardiac tamponade secondary to acute proximal thoracic aortic dissection, aneurysm, or heart rupture. Materials and methods: In a retrospective review of thoracoabdominal CT scans and records of 17 consecutive patients with hemopericardium, 10 patients with clinical signs of cardiac tamponade associated with hypodensity around the portal vein were selected; at surgery or autopsy all 10 patients had a thoracic aortic aneurysm, dissecting aneurysm, and heart rupture. The ratio of transverse inferior vena cava diameter to the diameter of the aorta at the level of the right adrenal gland was determined. Results: Abdominal CT scans showed focal (n = 1) or diffuse (n = 9) areas of hypodensity around the portal vein associated with pericaval hypodensity in four cases. Other abdominal abnormalities included persistent enhancement of renal parenchyma (n = 5), and gallbladder wall edema (n = 2); in nine cases the inferior vena cava diameter was significantly increased compared with that of patients with normal findings on CT scans (P 〈 0.01). Pathologic correlation, available in nine cases by autopsy, showed evidence of periportal lymphatic vessel dilatation and lymphedema. Conclusions: Periportal hypodensity on CT scans corresponds to the histopathologic picture of dilated lymphatic vessels or hepatic lymphedema; a diagnosis of hepatic lymphedema may be considered on the basis of CT scans in patients with pericardial effusion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s101400050032
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Complications of acute pancreatitis: helical CT evaluation (1999)
    Springer
    Emergency radiology 6 (1999), S. 113-120 
    Details
    ISSN: 1438-1435
    Keywords: Key words Computed tomography (CT) ; helical ; Pancreatitis ; Pancreas ; CT
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Acute pancreatitis represents one of the more commonly encountered etiologies of acute abdominal pain. Many complications can present emergently, including pancreatic abscess, necrosis, and hemorrhage. The purpose of this pictorial essay is to educate the reader about the spectrum of CT findings in patients with complications from pancreatitis. Emphasis is placed on evaluation with helical CT, stressing optimal technique and the imaging parameters essential for accurate diagnosis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s101400050035
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Imaging of ruptured abdominal and pelvic tumors (1999)
    Springer
    Emergency radiology 6 (1999), S. 204-209 
    Details
    ISSN: 1438-1435
    Keywords: Key words Neoplasms ; Hemorrhage ; Perforation ; CT ; Abdomen ; Pelvis.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Rupture of tumors is usually a critical and life-threatening condition. We demonstrate a wide variety of ruptured tumors with their imaging characteristics including gastric lymphoma, gastric leiomyosarcoma, leiomyosarcoma of the ileum, hepatocellular carcinoma, pancreatic pseudocyst, renal angiomyolipoma, renal cell carcinoma, ovarian endometrial cyst, ovarian corpus luteum cyst, and ovarian teratoma. Their imaging features are illustrated with an emphasis on clues to an accurate diagnosis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s101400050053
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    Computed tomography of traumatic and nontraumatic retroperitoneal emergencies (1999)
    Springer
    Emergency radiology 6 (1999), S. 232-243 
    Details
    ISSN: 1438-1435
    Keywords: Key words Abdomen ; CT ; Abdomen ; acute conditions ; Retroperitoneal space ; CT ; Retroperitoneal space ; injuries
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The retroperitoneum is a common location for injury following blunt or penetrating trauma. Additionally, there are a wide variety of nontraumatic emergencies that involve the retroperitoneum. Patients with these entities may present with symptoms ranging from acute abdominal or back pain to hypotension and/or fever. Computed tomography (CT) is the imaging modality of choice to evaluate acute processes of the retroperitoneum, as sonography has been historically less sensitive than CT. Knowledge of the compartments of the retroperitoneal space as well as the fascial planes is crucial to diagnose and understand the pathway of spread of pathology in this region. In this pictorial essay the spectrum of both traumatic and nontraumatic retroperitoneal emergencies will be demonstrated, as well as their effect on the different compartments of the retroperitoneum.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s101400050057
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 6
    Electronic Resource
    Electronic Resource
    Rupture of the pericardium with luxation of the heart after blunt trauma (1999)
    Springer
    Emergency radiology 6 (1999), S. 252-254 
    Details
    ISSN: 1438-1435
    Keywords: Key words Pericardium ; Rupture ; Heart luxation ; Chest X-ray ; CT
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract This report describes a patient who presented to our hospital in stable condition without signs of circulatory or respiratory failure following previous surgery for blunt abdominal trauma. The diagnosis of rupture of the pericardium with luxation of the heart was suggested on chest X-ray and CT. Left anterior thoracotomy revealed a 6-cm longitudinal left pericardial tear with herniation of the heart into the mediastinum.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s101400050060
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 7
    Electronic Resource
    Electronic Resource
    Spinal osteoblastoma: CT and MR imaging with pathological correlation (1999)
    Springer
    Skeletal radiology 28 (1999), S. 33-40 
    Details
    ISSN: 1432-2161
    Keywords: Key words Osteoblastoma ; Spine ; Radiography ; CT ; MRI ; Pathology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  Objectives. To illustrate the CT and MRI features of spinal osteoblastomas and correlate the imaging with histological findings. Design. In a retrospective review the CT and MRI features of spinal osteoblastomas with respect to mineralisation, signal intensity (SI), adjacent reactive changes, enhancement following gadolinium-DTPA (5 cases) and adjacent soft tissue masses were compared and correlated with the histological findings including the degree of osteoid formation and matrix mineralisation, vascularity and surrounding reactive changes in bone and soft tissue. Patients. Eleven patients (7 males and 4 females; age range 8–43 years, mean age 19.5 years) with 12 osteoblastomas (1 patient suffered a recurrence) were studied. Results. All lesions showed classical features on CT with varying degrees of matrix mineralisation, whereas MRI identified mineralisation in only eight of 12 cases. MRI showed low signal intensity of the lesion on both T1- and T2-weighted sequences in several cases in the absence of heavy mineralisation. In these cases, histological examination revealed diffuse osteoid production by the tumour. All patients given gadolinium showed enhancement within the tumour on MRI. Reactive bone marrow changes were identified on MRI in 10 cases, and in five of these the changes were at multiple levels. An adjacent soft tissue mass was demonstrated in five cases, but extraosseous tumour was present histologically in only two of these. Conclusions. The MRI appearances of spinal osteoblastomas are varied and show no characteristic features. MRI may also overestimate the extent of the lesion due to extensive reactive changes and adjacent soft tissue masses. CT should continue to be the investigation of choice for the characterisation and local staging of suspected spinal osteoblastomas.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002560050469
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 8
    Electronic Resource
    Electronic Resource
    Cryptococcoma of the sacrum (1999)
    Springer
    Skeletal radiology 28 (1999), S. 49-51 
    Details
    ISSN: 1432-2161
    Keywords: Key words Cryptococcoma ; sacrum ; Cryptococcoma ; immunocompromised ; MRI ; sacrum ; CT ; sacrum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  Cryptococcoma of the sacrum was the initial presentation of systemic cryptococcosis in a patient on chronic steroid therapy for autoimmune hepatitis. The bone lesion was the only overt manifestation of systemic cryptococcal disease, which preceded other clinical manifestations and led to the subsequent diagnosis of systemic infection.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002560050472
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 9
    Electronic Resource
    Electronic Resource
    Recurrent, multiple, calcified soft tissue metastases from osteogenic sarcoma without pulmonary involvement (1999)
    Springer
    Skeletal radiology 28 (1999), S. 710-713 
    Details
    ISSN: 1432-2161
    Keywords: Key words Osteosarcoma ; conventional ; Metastasis ; Soft tissues ; Retroperitoneum ; CT ; X-ray
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  Osteosarcoma (osteogenic sarcoma) metastasizes primarily to the lung. With the introduction of neoadjuvant chemotherapy as part of the treatment, the overall and disease-free survival rates have dramatically improved. In this case report, a young man with multiple soft tissue and bone metastases, including a rare large bone-forming retroperitoneal metastasis, is described. Despite the extensive extrapulmonary metastases, the patient did not develop pulmonary metastases in the 4 years following initial presentation of the primary tumour.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002560050578
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 10
    Electronic Resource
    Electronic Resource
    Progressive destructive vertebral sarcoid leading to surgical fusion (1999)
    Springer
    Skeletal radiology 28 (1999), S. 717-722 
    Details
    ISSN: 1432-2161
    Keywords: Key words Sarcoid ; vertebrae ; Sarcoid ; rib ; Sarcoid ; calvarium ; Osteolytic ; CT ; MRI ; Surgical fusion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  Skeletal sarcoidosis is rare. This report describes a 31-year-old African American man who presented with a destructive osteolytic lesion of C2 and another lesion in a rib. The lesion at C2 was treated with corpectomy and bone graft. Four months later the lesion recurred and a new lesion was discovered in the cervical vertebral column. The patient declined surgery for instability for another 3 months, choosing to remain in a halo. Seven months following the initial operation, a technetium bone scan showed spread of the disease to the calvarium and thoracic and lumbar vertebrae. The patient had no symptoms referable to these sites. The patient agreed to have his neck fused at this point. For the next 10 months, the patient was on steroids and a further new lesion appeared at L5 without localizing signs or symptoms. The patient declined further evaluation over the next 12 months and is now considered lost to follow-up.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002560050580
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 11
    Electronic Resource
    Electronic Resource
    Unusual longitudinal stress fractures of the femoral diaphysis: report of five cases (1999)
    Springer
    Skeletal radiology 28 (1999), S. 81-85 
    Details
    ISSN: 1432-2161
    Keywords: Key words Stress fractures ; CT ; MR imaging ; Radionuclide study ; Femur ; stress fractures
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  We present five cases of a distinctive type of longitudinal stress fracture of the upper femoral shaft in which the fracture line is parallel to the outer surface of the bone, in contrast to the perpendicular orientation to the cortical surface in previously reported cases of diaphyseal stress fractures. In two cases the fracture recurred after 15 and 18 months, respectively.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002560050478
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 12
    Electronic Resource
    Electronic Resource
    Extraskeletal osteochondroma of the foot (1999)
    Springer
    Skeletal radiology 28 (1999), S. 594-598 
    Details
    ISSN: 1432-2161
    Keywords: Key words Osteochondroma ; Chondroma ; Extraskeletal tumor ; Soft tissue tumor ; Foot ; CT ; MRI
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  A case of pathologically proven extraskeletal osteochondroma is presented with magnetic resonance imaging (MRI), computed tomography (CT), bone scan and radiographic findings. The diagnosis of extraskeletal osteochondroma should be considered when a discrete, ossified mass is localized in the soft tissues of the distal extremities. Nomenclature surrounding this entity is controversial and is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002560050626
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 13
    Electronic Resource
    Electronic Resource
    Malignant chondroblastoma presenting as a recurrent pelvic tumor with DNA aneuploidy and p53 mutation as supportive evidence of malignancy (1999)
    Springer
    Skeletal radiology 28 (1999), S. 644-650 
    Details
    ISSN: 1432-2161
    Keywords: Key words Chondroblastoma ; malignant ; recurrent ; p53 mutation ; Aneuploidy ; Pelvis ; Femur ; Xray ; CT
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  We report a rare case of malignant chondroblastoma, which presented in a 47-year-old man as a recurrent tumor, 18 years following wide excision of a typical pelvic chondroblastoma. Radiologic studies of the recurrent tumor showed a large, lytic, destructive lesion of the right pelvic bones and femur, with a pathologic fracture of the latter, a large pelvic soft tissue mass, and multiple pulmonary metastases. Biopsy tissue showed typical features of chondroblastoma, but also increased nuclear atypia, hyperchromasia, and pleomorphism, compared to the original tumor, and, most significantly, abnormal mitotic figures. Immunohistochemical studies of the recurrent tumor revealed p53 mutation and extensive proliferative activity, and flow cytometric studies showed DNA aneuploidy, none of which was present in the original tumor. The patient received chemotherapy and radiation, but died of disease eight months after presentation. We also review chondroblastoma in general, to assign this unusual lesion to a tumor subtype.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002560050567
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 14
    Electronic Resource
    Electronic Resource
    Proliferative myositis in a child (1999)
    Springer
    Skeletal radiology 28 (1999), S. 703-709 
    Details
    ISSN: 1432-2161
    Keywords: Key words Proliferative myositis ; Childhood ; Lumbar muscles ; Ossification ; X-ray ; CT ; MRI ; PET
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  A case of proliferative myositis in the lumbar paraspinal muscles in a 14-year-old boy is presented. Imaging investigations including plain radiograph, ultrasound, computed tomography (CT), magnetic resonance imaging (MRI), bone scan and positron emission tomography (PET) were suggestive of an inflammatory process such as myositis ossificans. The diagnosis was made by incisional biopsy. More pronounced edema, more muscle fiber necrosis and a higher cellularity were found compared to adult cases. The karyotype of the lesion was normal. Clinically, the mass disappeared spontaneously. After 24 months, asymptomatic bridging ossification between the third and fourth lumbar vertebrae was noted.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002560050577
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 15
    Electronic Resource
    Electronic Resource
    Chondrosarcoma of the foot: imaging, surgical and pathological correlation of three new cases (1999)
    Springer
    Skeletal radiology 28 (1999), S. 153-158 
    Details
    ISSN: 1432-2161
    Keywords: Key words Bone tumor ; Chondrosarcoma ; CT ; Magnetic resonance imaging ; Tumor of the ankle ; Tumor of the foot
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  The foot is an uncommon location for chondrosarcoma. The presentation, diagnosis, pathological findings, surgical treatment and follow-up of three patients with chondrosarcoma in this rare location are presented. Though nonspecific, MR imaging findings were of aid in the diagnosis and treatment planning of these patients. If the diagnosis of this tumor is rapidly made, a tumor excision instead of limb amputation may be sufficient treatment at surgery.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002560050492
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 16
    Electronic Resource
    Electronic Resource
    SAPHO syndrome: 20-year follow-up (1999)
    Springer
    Skeletal radiology 28 (1999), S. 159-162 
    Details
    ISSN: 1432-2161
    Keywords: Key words SAPHO syndrome ; CT ; Parosteal osteosarcoma ; Sternoclavicular joints
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  Considerable attention has been paid in the past 10 years to the radiological spectrum of disease entities belonging to the SAPHO syndrome. We report an unusual case presenting with an extra-axial (femoral) lesion, prior to description of this syndrome, which was radiologically and histologically mistaken for a parosteal osteosarcoma. Nineteen years later, a further lesion developed in the scapula together with the typical sternoclavicular manifestations, at which stage the correct diagnosis of SAPHO syndrome was established.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002560050493
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 17
    Electronic Resource
    Electronic Resource
    Isolation of RNA and Protein from Guard Cells of Nicotiana glauca (1999)
    Springer
    Plant molecular biology reporter 17 (1999), S. 371-383 
    Details
    ISSN: 1572-9818
    Keywords: epidermal peel ; extraction ; gene expression ; stomata ; tree tobacco
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Stomatal guard cells are critical for maintenance of plant homeostasis and represent an interesting cell type for studies of leaf cell differentiation and patterning. Here we describe techniques for the isolation of guard cell RNA and protein from blended epidermal peels of Nicotiana glauca. The RNA isolation procedure is a modification of the hot borate method, which is particularly well-suited for recalcitrant tissues. Protein was extracted by disrupting guard cell-enriched epidermis with a French® press. This system offers the following advantages: relatively high yield, low or no contamination by other cell types, fresh tissue as a source of RNA and protein rather than protoplasts, and a plant species that is readily transformable. These techniques will allow for cloning and analysis of genes expressed in guard cells, application of traditional biochemical techniques to guard cell proteins, as well as characterization of genetic manipulation of guard cell function in transgenic plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1007602017492
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 18
    Electronic Resource
    Electronic Resource
    PPARγ, the ultimate thrifty gene (1999)
    Springer
    Diabetologia 42 (1999), S. 1033-1049 
    Details
    ISSN: 1432-0428
    Keywords: Keywords Adipogenesis ; adipose tissue ; cofactors ; gene expression ; fatty acids ; insulin resistance ; nuclear receptors ; prostaglandin ; thiazolidinediones ; Type II diabetes ; transcription.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The peroxisome proliferator-activated receptor gamma (PPARγ) quickly evolved over the last decade from a new orphan receptor to one of the best characterized nuclear receptors. This fast pace in PPARγ research was triggered by two main discoveries. Firstly, that PPARγ was shown to have a key role in adipogenesis and be a master controller of the “thrifty gene response” leading to efficient energy storage. Secondly, the discovery that its synthetic ligands, the thiazolidinediones, are promising insulin sensitizing drugs, which are currently being developed for the treatment of Type II (non-insulin-dependent) diabetes mellitus. More recently this nuclear receptor emerged from a role limited to metabolism (diabetes and obesity) to a power player in general transcriptional control of numerous cellular processes, with implications in cell cycle control, carcinogenesis, inflammation, atherosclerosis and immunomodulation. This widened role of PPARγ will certainly initiate a new flurry of research, which will not only refine our current often partial knowledge of PPARγ but more importantly also establish that this receptor has a definite role as a primary link adapting cellular, tissue and whole body homeostasis to energy stores. [Diabetologia (1999) 42: 1033–1049]
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001250051268
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 19
    Electronic Resource
    Electronic Resource
    Uncoupling protein-3 gene expression: reduced skeletal muscle mRNA in obese humans during pronounced weight loss (1999)
    Springer
    Diabetologia 42 (1999), S. 302-309 
    Details
    ISSN: 1432-0428
    Keywords: Keywords Obesity ; genetics ; uncoupling protein-3 ; gene expression ; skeletal muscle.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Aims: Uncoupling protein-3 is a member of a protein family that serves to dissipate energy in the form of heat thereby modulating energy expenditure. Alternative processing of uncoupling protein-3 transcripts results in two mRNA species that encode a large and small protein, perhaps differing in functional activity. Since obesity is associated with disrupted energy homeostasis, we measured muscle mRNA expression in morbidly obese and lean subjects. Methods: The two uncoupling protein-3 mRNA species were quantified in muscle tissue using an RNase protection assay. Gene locus effects on mRNA expression were studied by quantitative allele-specific primer extension. Results: In both obese and lean subjects, the mRNA species encoding the small protein isoform was twice as abundant as the mRNA species encoding the large protein isoform. Neither the total uncoupling protein-3 mRNA expression nor the molar abundance ratios of the two mRNA species differed between obese and lean male or female subjects. Women who had lost 37 ± 22 kg of weight in response to dietary restriction and continued a hypocaloric diet displayed lower mRNA than obese (p 〈 0.005) or lean women (p 〈 0.05). Primer extension assays in lean and obese subjects showed similar allelic mRNA abundance in all but one subject studied. Conclusion: Muscle expression of the two uncoupling protein-3 mRNA species is similar in obese and lean people. In obese patients, prolonged hypocaloric diet downregulates uncoupling protein-3 mRNA expression in muscle and can thereby enhance its energy efficiency. Sequence substitutions at the gene locus may only be minor determinants of mRNA expression in muscle tissue. [Diabetologia (1999) 42: 302–309]
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001250051155
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 20
    Electronic Resource
    Electronic Resource
    Regulation of UCP2 and UCP3 by muscle disuse and physical activity in tetraplegic subjects (1999)
    Springer
    Diabetologia 42 (1999), S. 826-830 
    Details
    ISSN: 1432-0428
    Keywords: Keywords Uncoupling proteins ; exercise ; tetraplegia ; skeletal muscle ; mRNA ; gene expression ; polymerase chain reaction.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Aims/hypothesis. The regulation of uncoupling protein 2 and uncoupling protein 3 gene expression in skeletal muscle has recently been the focus of intense interest. Our aim was to determine expression of uncoupling protein 2 and 3 in skeletal muscle from tetraplegic subjects, a condition representing profound muscle inactivity. Thereafter we determined whether exercise training would modify expression of these genes in skeletal muscle. Methods. mRNA expression of uncoupling protein 2 and 3 was determined using quantitative reverse transcription-polymerase chain-reaction. Results. Expression of uncoupling protein 2 and 3 mRNA was increased in skeletal muscle from tetraplegic compared with able-bodied subjects (3.7-fold p 〈 0.01 and 4.1-fold, p 〈 0.05, respectively). A subgroup of four tetraplegic subjects underwent an 8-week exercise programme consisting of electrically-stimulated leg cycling (ESLC, 7 ESLC sessions/week). This training protocol leads to increases in whole body insulin-stimulated glucose uptake and expression of genes involved in glucose metabolism in skeletal muscle from tetraplegic subjects. After ESLC training, uncoupling protein 2 expression was reduced by 62 % and was similar to that in able-bodied people. Similarly, ESLC training was associated with a reduction of uncoupling protein 3 expression in skeletal muscle from three of four tetraplegic subjects, however, post-exercise levels remained increased compared with able-bodied subjects. Conclusion/interpretation. Tetraplegia is associated with increased mRNA expression of uncoupling protein 2 and 3 in skeletal muscle. Exercise training leads to normalisation of uncoupling protein 2 expression in tetraplegic subjects. Muscle disuse and physical activity appear to be powerful regulators of uncoupling protein 2 and 3 expression in human skeletal muscle. [Diabetologia (1999) 42: 826–830]
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001250051233
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 21
    Electronic Resource
    Electronic Resource
    Diffuse metastatic infiltration of a carcinoma into skeletal muscle (1999)
    Springer
    European radiology 9 (1999), S. 208-210 
    Details
    ISSN: 1432-1084
    Keywords: Key words: Muscles ; diseases ; Neoplasms ; CT
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. Skeletal muscle is one of the most unusual sites of metastasis from any malignancy. We report a patient with rapidly progressive contractures due to metastatic infiltration of a carcinoma of unknown origin into the skeletal muscle. This 61-year-old man presented with a 1-month history of rapidly evolving, painful restriction of mobility of his right arm and his legs. Computed tomography showed diffuse metastatic nodules in all muscles, particularly in the hip abductors. Muscle biopsy revealed extensive infiltration of the muscle with carcinoma cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003300050657
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 22
    Electronic Resource
    Electronic Resource
    Cryptorchidism: strategies in detection (1999)
    Springer
    European radiology 9 (1999), S. 336-343 
    Details
    ISSN: 1432-1084
    Keywords: Key words: Cryptorchidism ; Scrotum ; US ; CT ; MRI ; Testis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. Imaging evaluation of the patient with a non-palpable testis has evolved over recent decades. The rational explanation of imaging in these patients requires a clear understanding of the various causes of a non-palpable testis, and an appreciation of the utility and limitations of the available imaging modalities. This review describes the classification of non-palpable testis and discusses the role of modern imaging in evaluation. In particular, the relative accuracies of ultrasound, CT and MRI is reviewed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003300050676
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 23
    Electronic Resource
    Electronic Resource
    CT of blunt trauma of the pancreas in adults (1999)
    Springer
    European radiology 9 (1999), S. 244-249 
    Details
    ISSN: 1432-1084
    Keywords: Key words: Pancreas ; CT ; Injuries ; Surgery
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. In order to describe the CT findings in pancreatic injury and to evaluate the sensitivity of this technique, we performed a retrospective study. During a 5-year period (1993–1997), eight patients (five males and three females; age range 10–47 years) were investigated with CT. Endoscopicretrograde cholangiopancreatography (ERCP) was obtained in two patients, pre- and intra-operatively, respectively. Among the standard laboratory tests obtained at admission, the value of serum amylase was reviewed. The imaging findings, especially those obtained with CT, were correlated with the surgical findings, when available (in seven of eight patients). At admission, diagnosis of pancreatic injury was missed at CT in three of eight patients (37.5 %); thus, the sensitivity of CT for pancreatic injury was 62.5 %. ERCP showed rupture of the pancreatic duct in the two cases in which it was performed. Serum amylase was elevated at admission in four of eight patients, resulting in a sensitivity of 50 %. After surgery, an enterocutaneous fistula developed in one case, and was managed conservatively. One patient died from brain injury. Proper implementation of the CT technique and accurate film reading is mandatory to establish the diagnosis of pancreatic contusion. No correlation between CT features and type of outcome of surgical management could be established. On retrospective review of the CT examinations, it appeared that two of the three false-negative results could have been avoided. Therefore, proper CT technique and accurate film reading are mandatory in establishing the diagnosis of pancreatic injury.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003300050662
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 24
    Electronic Resource
    Electronic Resource
    Mehrzeilen-Spiral-CT in der Diagnostik von Pankreastumoren (1999)
    Springer
    Der Radiologe 39 (1999), S. 958-964 
    Details
    ISSN: 1432-2102
    Keywords: Schlüsselwörter Spiral-CT ; Mehrzeilen-Spiral-CT ; Pankreaskarzinom ; Tumorstaging ; 2D-/3D-Bildgebung ; Key words Computed tomography ; Computed tomography ; helical ; Computed tomography ; technology ; Computed tomography ; thin-section ; Pancreatic neoplasms ; CT
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary Purpose. Investigation of the capabilities of MSCT and its value for the staging of pancreatic carcinomas. Methods. 50 Patients with suspected pancreatic carcinoma were examined with a biphasic multislice-spiral-CT protocol: slice collimation 4×1 mm, Pitch 3.5–4 mm. After administation of 120 ml contrast medium and 50 ml NaCl with a flow rate of 3.0 ml/s the examination was started with a delay of 40 s (pancreatic phase) and 80 s (portalvenous phase). Results. Multislice spiral CT allows the examination of the whole upper abdomen with nearly isotropic data sets. This is the premise for the optimal assessment of the tumor extent in all planes, excellent demarcation of the tumor against the adjacent vessels and organs and the demarcation of small peripancreatic lymph nodes. Conclusions. Multislice spiral CT and the use of interactive multiplanar reconstructions improve the staging of pancreatic cancer.
    Notes: Zusammenfassung Ziel unserer Untersuchungen war es, die Möglichkeiten der Mehrzeilen-Detektor-Spiral-CT und ihre Bedeutung für das Staging von Pankreastumoren zu evaluieren. Bei insgesamt 50 Patienten, bei denen der Verdacht auf ein Pankreaskarzinom bestand, wurde im Rahmen der Tumorstagings ein biphasisches hochaufgelöstes Mehrzeilen-Spiral-CT mit einer Schichtkollimation von 4×1 mm, einem Pitch von 3,5–4, 120 ml Kontrastmittel, 50 ml 0,9%NaCl-Bolus, 3,0 ml/s Fluß und einem Startdelay von durchschnittlich 40 s (Pankreasparenchymphase) und 80 s (portalvenöse Phase) durchgeführt. Die Mehrzeilen-Spiral-CT ist in der Lage die gesamte Pankreasloge und auch die angrenzenden Organe mit hoher Ortsauflösung in allen Raumebenen abzubilden. Die nahezu isotrope multiplanare Bildgebung erlaubt die vollständige Erfassung der Tumorausdehnung in allen Raumebenen und eine bessere Abgrenzung der Tumoren gegenüber dem angrenzenden Fettgewebe, den benachbarten Organen (Gefäße, Duodenum, Magen) und einen sichereren Nachweis von peripankreatischen Lymphknoten. Die Mehrzeilen-Spiral-CT und der Einsatz von interaktiven multiplanaren Rekonstruktionen verbessern nachhaltig die Bestimmung der Ausdehnung von Pankreaskarzinomen.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001170050588
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 25
    Electronic Resource
    Electronic Resource
    Dental-CT: Untersuchungstechnik, Strahlenbelastung und Anatomie (1999)
    Springer
    Der Radiologe 39 (1999), S. 1027-1034 
    Details
    ISSN: 1432-2102
    Keywords: Schlüsselwörter Computertomographie ; Kiefer ; Dental-CT ; Software ; Key words Mandible ; Maxilla ; Computed tomography ; Software ; Dental-CT ; Jaws ; CT
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary Traditionally oral surgeons and dentists have evaluated the jaws using intraoral films and panoramic radiographs. The involvement of radiologists has been limited. In the past few years dedicated CT-software-programs developed to evaluate dental implant patients have provided a new look at the jaws. The complex anatomy is described and identified on human skulls and on axial, panoramic, and cross-sectional images. With this anatomic description Dental-CT-scans are used to demonstrate the anatomy of maxilla and the mandible. An overview of the technique of Dental-CT is provided, furthermore the radiation dose of different organs is explained. Suggestions to reduce these doses by simple modifications of the recommended procols are given.
    Notes: Zusammenfassung Die Einführung im Bereich der Computertomographiesoftware (Dental-CT) ermöglicht dem Radiologen zusätzlich zu den üblichen, von den Zahnärzten durchgeführten Röntgenuntersuchungen eine überlagerungs- und verzerrungsfreie Darstellung des Ober- und Unterkiefers. In der Implantologie ist mit dieser Darstellung eine exakte Planung möglich. Weiterhin haben sich Dünnschicht-CT-Untersuchungen auch bei der Abklärung von Zysten, Tumoren, Frakturen, tiefen Parodontitiden und retinierten Zähnen bewährt. In dieser Arbeit wird ein Überblick über die Anatomie, die Untersuchungstechnik des Dental-CT und die auftretende Strahlenbelastung gegeben. Basierend auf rezente Literaturangaben kann eine Reduktion der absorbierten Dosis bei gleichbleibender Bildqualität durch einfache Protokollmodifikationen erzielt werden.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001170050598
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 26
    Electronic Resource
    Electronic Resource
    Imaging findings in Langerhans' cell histiocytosis of the liver and the spleen in an adult (1999)
    Springer
    European radiology 9 (1999), S. 96-98 
    Details
    ISSN: 1432-1084
    Keywords: Key words: Langerhans' cell histiocytosis ; Liver ; Spleen ; CT ; Ultrasound
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. We present a case of Langerhans' cell histiocytosis (LCH) of the liver and spleen in an adult. The imaging features are different from those in the few previously reported cases of individual organ involvement by LCH.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003300050636
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 27
    Electronic Resource
    Electronic Resource
    A case of bleeding gastric lipoma: US, CT and MR findings (1999)
    Springer
    European radiology 9 (1999), S. 256-258 
    Details
    ISSN: 1432-1084
    Keywords: Key words: Stomach neoplasm ; Gastric lipoma ; Acute gastrointestinal hemorrhage ; Ultrasound ; CT ; MR imaging
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. We report a case of gastric lipoma which manifested with an episode of acute gastrointestinal hemorrhage. Preoperative diagnosis was based on the US, CT, and MRI findings, as the results of gastrointestinal endoscopy were inconclusive. The role of current imaging methods, and particularly of MRI, is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003300050664
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 28
    Electronic Resource
    Electronic Resource
    Multiple hepatic angiolipomas: a case report and review of literature (1999)
    Springer
    European radiology 9 (1999), S. 259-261 
    Details
    ISSN: 1432-1084
    Keywords: Key words: Liver neoplasms ; CT ; Fat ; CT
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. Follow-up of two hepatic angiolipomas in a patient without evidence of tuberous sclerosis is reported. Initially, the lesions presented as homogenously enhancing masses, which were nearly isodense to normal liver tissue on plain CT scans. Focal nodular hyperplasia was assumed. One year later, fat was detected in the growing tumors and percutaneous core biopsy revealed hepatic angiolipomas. Natural history of these rare lesions is unknown, and this is to the best of our knowledge the first observation of fatty metamorphosis in such a benign, mesenchymal hepatic neoplasm.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003300050665
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 29
    Electronic Resource
    Electronic Resource
    Renal trauma in occult ureteropelvic junction obstruction: CT findings (1999)
    Springer
    European radiology 9 (1999), S. 611-615 
    Details
    ISSN: 1432-1084
    Keywords: Key words: Kidney ; Ureteropelvic junction obstruction ; CT ; Trauma
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. The aim of this study was to present CT findings of occult ureteropelvic junction obstruction in patients with renal trauma and to describe the clinical signs and singular CT features that are characteristically observed with trauma and are relevant to management of these patients. We retrospectively reviewed 82 helical CT studies in patients with renal trauma referred to our institution. We found 13 cases of occult preexisting renal pathology, six of which were occult ureteropelvic junction obstructions. The clinical presentation, radiologic findings of trauma according to the Federle classification, and CT findings of obstructed ureteropelvic junction are presented. We found three category-I lesions (one in a horseshoe kidney), two of them treated with nephrostomy because of increased ureteropelvic junction obstruction due to pelvic clots; two category-II lesions (parenchymal and renal pelvis lacerations) that had presented only with microhematuria; and one category-IV lesion (pelvic laceration alone). Pelvic extension was demonstrated in all the cases with perirenal collections. The CT studies in all the cases with suspected ureteropelvic junction obstruction showed decreased parenchymal thickness and enhancement, and dilatation of the renal pelvis and calyx, with a normal ureter. Computed tomography can provide information to confidently diagnose underlying ureteropelvic junction obstruction in renal trauma, categorize the traumatic injury (at times clinically silent) and facilitate proper management according to the singularities observed, such us rupture of the renal pelvis alone (Federle category IV) and increasing ureteropelvic obstruction due to clots which can be decompressed by nephrostomy.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003300050718
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 30
    Electronic Resource
    Electronic Resource
    Tumor diagnosis in the adult liver transplant candidate (1999)
    Springer
    European radiology 9 (1999), S. 841-852 
    Details
    ISSN: 1432-1084
    Keywords: Key words: Liver transplantation ; Hepatocellular carcinoma ; Cholangiocarcinoma ; MR Imaging ; CT ; Ultrasound
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. Hepatic transplantation has emerged as a potentially curative treatment of certain malignant hepatic neoplasms such as hepatocellular carcinoma, bile duct carcinoma, fibrolamellar hepatocellular carcinoma, metastases from neuroendocrine tumors, and epithelioid hemangioendothelioma. In the early years of hepatic transplantation, there was great enthusiasm to cure patients with unresectable hepatobiliary malignancy. This early enthusiasm was tempered by the unfavorable outcome of transplantation in advanced cases of malignancy and the organ-donor shortage. Presently, patients have to be selected with predictable likelihood for long-term survival. Pre-transplantation imaging is indispensable for detection, characterization, staging, and surgical road-mapping before the procedure. The present article focuses on the role of imaging modalities in these different aspects of preoperative assessment.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003300050754
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 31
    Electronic Resource
    Electronic Resource
    Pathways of lymph node involvement in upper abdominal malignancies: evaluation with high-resolution CT (1999)
    Springer
    European radiology 9 (1999), S. 868-874 
    Details
    ISSN: 1432-1084
    Keywords: Key words: Abdomen ; Neoplasms ; CT ; Lymphatic system ; Anatomy ; Metastases ; Omentum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. The aim of this study was to enhance our understanding of the pathways of lymphatic spread of primary carcinomas in the upper abdomen by recognizing the development, configuration, and frequency of nodal enlargement in discrete anatomic regions. The study included 417 patients with histologically confirmed carcinomas (CC) of the stomach (n = 267), liver (n = 98), gallbladder (n = 25), and bile ducts (n = 27). All patients were studied by high-resolution CT and tumor extension to the lymph nodes of the subperitoneal space was clearly identified in 59 patients [33 with CC of the stomach, 8 with CC of the gallbladder, 3 with CC of the bile ducts, and 15 with hepatocellular carcinoma (HCC)]. In 47 of 59 patients this extension was confirmed by surgery or aspiration biopsy. Three discrete anatomic groups of lymph nodes were recognized producing a relatively distinct CT configuration when involved: (a) the hepatoduodenal seen in 49 patients; (b) the peripancreatic demonstrated in 33 patients; and (c) the aortocaval recognized in 16 patients. These groups of lymph nodes can be seen individually involved or in combination. Recognition of involvement of these nodes is important for correct diagnosis and staging of upper abdominal malignancies. The development of this involvement follows the natural flow of lymph via the lesser omentum to the retroperitoneal space.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003300050757
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 32
    Electronic Resource
    Electronic Resource
    Osteomyelitis: a review of currently used imaging techniques (1999)
    Springer
    European radiology 9 (1999), S. 894-900 
    Details
    ISSN: 1432-1084
    Keywords: Key words: Osteomyelitis ; Radiography ; Bone scintigraphy ; CT ; Ultrasound ; MR imaging
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. Conventional radiographs remain the initial imaging modality involved in the diagnosis of osteomyelitis. Bone scintigraphy and its specific agents did not only eliminate the problems of inherent low sensitivity of conventional radiographs, but also increased the specificity to higher degrees. Spiral CT, on the other hand, has solved several diagnostic problems, such as osteomyelitis of the sterno-clavicular junction and hidden areas in the pelvic bones. Magnetic resonance imaging with its multiplanar capability, greater anatomic details and excellent soft tissue bone marrow contrast resolution has a significant role in surgical planning and limb preservation. Ultrasound and US-guided aspiration has recently been involved in the diagnosis and management of osteomyelitis with several advantages particularly in children. Our goal in this review is to outline the ability of various imaging techniques by comparing their strengths and weaknesses in the diagnosis of osteomyelitis. Finally, we suggest various imaging algorithms for specific clinical scenarios. Spondylitis and septic arthritis are not discussed in this review.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003300050763
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 33
    Electronic Resource
    Electronic Resource
    Endovascular treatment of superior vena cava obstruction in patients with malignancies (1999)
    Springer
    European radiology 9 (1999), S. 965-971 
    Details
    ISSN: 1432-1084
    Keywords: Key words: Vena cava ; Stenosis ; Obstruction ; Interventional procedure ; Neoplasm ; Grafts and prosthesis ; Thrombolysis ; CT
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. The aim of this study was to report our experience on the management of superior vena cava obstruction (SVCO) secondary to malignant disease, using endovascular procedures. Twenty-six patients with SVCO due to primary or secondary tumors of the lung or the mediastinum, or catheter inserted for treatment of an extra-thoracic neoplasm, had an endovascular therapy which consisted of stenting, angioplasty, thrombo-aspiration or local fibrinolysis. Immediately after the procedure, rapid relief of symptoms occurred in 24 (90 %) of the patients. The mean Kishi's score decreased from 5.5 to 0.96. Immediate complications included one death related to pericarditis bleeding following fibrinolysis. Three patients relapsed after 20 days, 4 months and 6 months, and needed a second stenting. At 6 months the primary patency rate was 83 % and the secondary patency rate was 89 %. Endovascular treatment of SVCOs is a simple and safe procedure to restore the patency of the superior vena cava in malignant SVCO. It should be indicated in most cases as first-line treatment and performed as early as possible.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003300050777
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 34
    Electronic Resource
    Electronic Resource
    Right diaphragmatic rupture and hepatic hernia: an indirect sign on computed tomography (1999)
    Springer
    European radiology 9 (1999), S. 972-974 
    Details
    ISSN: 1432-1084
    Keywords: Key words: Diaphragm ; Hepatic hernia ; Blunt trauma ; CT
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. We report a case of blunt traumatic right diaphragm rupture with hepatic hernia. The diagnosis was first suggested by an abnormal hepatic location depicted on axial CT. This finding can be considered as a potentially new indirect sign of right diaphragm rupture in patients with blunt trauma. The diagnosis was then confirmed by reformatted CT and MR images.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003300050778
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 35
    Electronic Resource
    Electronic Resource
    Calcific tendinitis of the gluteus maximus tendon: CT findings (1999)
    Springer
    European radiology 9 (1999), S. 1104-1106 
    Details
    ISSN: 1432-1084
    Keywords: Key words: Tendinitis ; CT ; Tendons ; Calcification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. Two cases of calcific tendinitis of gluteus maximus muscle are presented. The CT findings, including amorphous calcification without soft tissue mass and possible cortical erosion at the femoral enthesis of the gluteus maximus muscle, are highly suggestive of calcific tendinitis at this unusual but classical location. Ossifying entheses with well-defined cortical defect are frequent at the femoral insertion of the gluteus maximus muscle in asymptomatic subjects and must be differentiated from a real cortical erosion sometimes associated with these calcific tendinitis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003300050799
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 36
    Electronic Resource
    Electronic Resource
    Multifocal bone tuberculosis presenting as a breast mass: CT and MRI findings (1999)
    Springer
    European radiology 9 (1999), S. 1117-1119 
    Details
    ISSN: 1432-1084
    Keywords: Key words: Tuberculosis ; Skeletal ; CT ; MR imaging
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. Chest wall involvement is an uncommon manifestation of musculoskeletal tuberculosis. We present computed tomography and magnetic resonance imaging findings in a case with multifocal musculoskeletal tuberculosis presenting as a breast mass. These radiological modalities are not diagnostic without histopathological confirmation, but they are valuable guides to surgery in defining the extent of disease involvement.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003300050802
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 37
    Electronic Resource
    Electronic Resource
    Exogenous lipoid pneumonia: HRCT, MR, and pathologic findings (1999)
    Springer
    European radiology 9 (1999), S. 1190-1196 
    Details
    ISSN: 1432-1084
    Keywords: Key words: Lipoid pneumonia ; Diagnosis ; Lung ; Disease ; CT ; MRI
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. The objective of this study was to describe high-resolution CT (HRCT) and MR findings of exogenous lipoid pneumonia and to correlate them with pathologic findings. A retrospective review of the medical records of our institution revealed seven patients with a diagnosis of lipoid pneumonia based on clinical data, chest films, bronchoalveolar lavage, and follow-up. Both HRCT and MR imaging were reviewed by two readers. Pathologic examination of the resected specimen or surgical biopsies were also reviewed in the four available cases. The HRCT findings were pulmonary consolidations (n = 6) with fatty (n = 3) or unspecific but low attenuation values (n = 3), areas of ground-glass opacities (n = 5), septal lines, and centrilobular interstitial thickening (n = 5). In five of the seven cases, a crazy-paving pattern of various spread was also present, either isolated (n = 1) or surrounding a pulmonary consolidation. In two cases traction bronchiectasis and cystic changes consistent with fibrosis were seen. At MR imaging (n = 2) a pulmonary consolidation of high signal intensity on T1-weighted image consistent with lipid content was present in one case. Pathologic examination (n = 4) showed the coexistence of lobules with lesions of various ages, sometimes in contiguous lobules, within the same patient. Recent lesions were those with alveolar fill-in by spumous macrophages and almost normal alveolar walls and septae. In more advanced lesions, lobules were filled in with larger vacuoles often surrounded by inflammatory infiltrates of alveolar walls, bronchiolar walls, and septa. The oldest lesions were characterized by fibrosis and parenchymal distortion around large lipid-containing vacuoles. The HRCT findings reflect pathologic findings in exogenous lipoid pneumonia. Although non-specific, consolidation areas of low attenuation values and crazy-paving pattern are frequently associated in exogenous lipoid pneumonia and are indicative of the diagnosis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003300050815
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 38
    Electronic Resource
    Electronic Resource
    Inflammatory myofibroblastic tumor of the lung (inflammatory pseudotumor): uncommon cause of solitary pulmonary nodule (1999)
    Springer
    European radiology 9 (1999), S. 1205-1207 
    Details
    ISSN: 1432-1084
    Keywords: Key words: Lung nodule ; CT ; Inflammatory pseudotumor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. Lung inflammatory myofibroblastic tumor is an uncommon cause of solitary lung nodule (0.7 % of lung tumors). The principal site of inflammatory pseudotumor is the lung, but it can also occur elsewhere in various organs. Although benign, they may be locally very aggressive. Recurrent and multifocal forms have been described. Since they are likely to mimic malignant neoplasms, accurate histopathologic diagnosis is necessary in order to guide appropriate surgical excision and avoid aggressive treatments. We report a case of lung involvement in a young adult with radiologic and pathologic correlations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003300050818
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 39
    Electronic Resource
    Electronic Resource
    Castleman disease: CT and MR imaging features of a retroperitoneal location in association with paraneoplastic pemphigus (1999)
    Springer
    European radiology 9 (1999), S. 1219-1221 
    Details
    ISSN: 1432-1084
    Keywords: Key words: Castleman disease ; CT ; MR imaging ; Paraneoplastic pemphigus ; Abdomen ; Neoplasms
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. We describe the CT and MRI features of a case of Castleman disease which was unusual by both its retroperitoneal location and its association with paraneoplastic pemphigus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003300050821
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 40
    Electronic Resource
    Electronic Resource
    Chest wall infiltration by lung cancer: value of thin-sectional CT with different reconstruction algorithms (1999)
    Springer
    European radiology 9 (1999), S. 1304-1309 
    Details
    ISSN: 1432-1084
    Keywords: Key words: Lung neoplasms ; Staging ; Pleura ; CT
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. The aim of this investigation was to evaluate whether thin-sectional CT with different reconstruction algorithms can improve the diagnostic accuracy with regard to chest wall invasion in patients with peripheral bronchogenic carcinoma. Forty-one patients with intrapulmonary lesions and tumor contact to the thoracic wall as seen on CT staging underwent additional 1-mm CT slices with reconstruction in a high-resolution (HR) and an edge blurring, soft detail (SD) algorithm. Five criteria were applied and validated by histological findings. Using the criteria of the intact fat layer, HRCT had a sensitivity of 81 % and a specificity of 79 %, SD CT had a sensitivity of 96 % and a specificity of 78 %, and standard CT technique had a sensitivity of 50 % and a specificity of 71 %, respectively. Regarding changes of intercostal soft tissue, HRCT achieved a sensitivity of 71 % and a specificity of 96 %, SD CT had a sensitivity of 94 % and a specificity of 96 % (standard CT technique: sensitivity 50 % and specificity 96 %) . For the other criteria, such as pleural contact area, angle, and osseous destruction, no significant differences were found. Diagnostic accuracy of chest wall infiltration can be improved by using thin sectional CT. Especially the application of an edge-blurring (SD) algorithm increases sensitivity and specificity without additional costs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003300050837
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 41
    Electronic Resource
    Electronic Resource
    “Air bubble”: a new diagnostic CT sign of perforated pulmonary hydatid cyst (1999)
    Springer
    European radiology 9 (1999), S. 1321-1323 
    Details
    ISSN: 1432-1084
    Keywords: Key words: Hydatid disease ; Chest ; CT
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. The greatest difficulty in the CT diagnosis of perforated pulmonary hydatid cyst (PPHC) is the increase in the attenuation numbers following infection. Because of the solid density of infected hydatid cysts, the differentiation from an abscess or neoplasm is usually impossible. The aim of this study was to evaluate the value of “air bubble” as a new CT sign in the diagnosis of PPHC. Sixty-five patients (28 men and 37 women) with PPHC were included in the study. As a control group, 55 patients who had malignant (n = 36) or non-malignant (n = 19) pulmonary diseases were also examined. Radiological diagnosis with classical CT findings was made in only 38 of 65 patients (58.5 %) with PPHC. Air bubble sign was positive in 54 of the patients with PPHC (sensitivity 83.1 %) but only 3 of 55 patients in control group (specificity 94.5 %). When we analyzed the CT scans with classical CT findings including air bubble, the diagnosis of PPHC was made in 61 of patients (93.8 %). It is concluded that “air bubble sign” is a valuable CT finding in the diagnosis of PPHC.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003300050840
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 42
    Electronic Resource
    Electronic Resource
    Sclerosing stromal tumor of the ovary: radiologic findings (1999)
    Springer
    European radiology 9 (1999), S. 1335-1338 
    Details
    ISSN: 1432-1084
    Keywords: Key words: Sclerosing stromal tumor ; MR imaging ; CT ; Dynamic study ; Ovarian tumor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. Sclerosing stromal tumor is a rare ovarian neoplasm. We describe the radiologic findings of sclerosing stromal tumor in two patients. In both patients, MR and CT images showed a large mass in the left adnexal region. On dynamic contrast-enhanced images, the tumors showed early peripheral enhancement with centripetal progression.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003300050844
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 43
    Electronic Resource
    Electronic Resource
    Imaging findings in patients with myelofibrosis (1999)
    Springer
    European radiology 9 (1999), S. 1366-1375 
    Details
    ISSN: 1432-1084
    Keywords: Key words: Myelofibrosis ; Plain film ; Ultrasound ; CT ; MR imaging ; Extramedullary hematopoiesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. The purpose of this review is to illustrate the wide range of radiological abnormalities in myelofibrosis. Myelofibrosis, also called myeloid metaplasia, is a myeloproliferative disorder of unknown etiology. The common imaging findings in patients with myelofibrosis are osteosclerosis, hepatosplenomegaly, and lymphadenopathies. In addition, extramedullary hematopoiesis may develop in multiple sites such as chest, abdomen, pelvis, and central nervous system, simulating malignant disease. Selected plain-film, CT, and MR images in patients with myelofibrosis are shown as pictorial essay to allow ready recognition of the most common imaging abnormalities of the disease.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003300050850
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 44
    Electronic Resource
    Electronic Resource
    Presacral extramedullary haematopoiesis with involvement of the sciatic nerve (1999)
    Springer
    European radiology 9 (1999), S. 1404-1406 
    Details
    ISSN: 1432-1084
    Keywords: Key words: Blood diseases ; CT ; Extramedullary haematopoiesis ; Presacral mass
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. We present the case of a 60-year-old woman with no known blood disease who developed an extramedullary haematopoiesis of presacral localization that affected the right sciatic nerve. The diagnosis was made with imaging studies and CT-guided fine-needle aspiration.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003300050857
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 45
    Electronic Resource
    Electronic Resource
    Esophageal varices before and after endoscopic variceal ligation: evaluation using helical CT (1999)
    Springer
    European radiology 9 (1999), S. 1546-1549 
    Details
    ISSN: 1432-1084
    Keywords: Key words: Esophageal varices ; CT ; Endoscopic variceal ligation ; MPR ; 3D image
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. The purpose of this study was to demonstrate the utility of helical CT in assessing the therapeutic effects of endoscopic variceal ligation (EVL). Twenty-four patients with esophageal varices were examined. Helical scanning was initiated 60 s after intravenous injection (Iopamidol 300 mgI/ml, total 120 ml, 3 ml/s) was started. Esophageal varices were clearly depicted as high-density areas. Multiplanar reformation and 3D images demonstrated collateral circulation three-dimensionally. After EVL, mucosal high-density areas had diminished markedly, but collateral veins around the esophagus, and gastro- and/or spleno-renal shunts, were unchanged in all patients. Of 21 patients with collateral circulation, esophageal varices recurred endoscopically in 6 patients within 12 months. In 3 patients without collateral circulation, esophageal varices did not recur within 12 months. From these findings, we conclude that helical CT is a useful method for assessing the therapeutic effects of EVL.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003300050881
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 46
    Electronic Resource
    Electronic Resource
    Anomalous origin of the left coronary artery arising from the pulmonary trunk: report of an adult case with long-term follow-up after surgery (1999)
    Springer
    European radiology 9 (1999), S. 1570-1573 
    Details
    ISSN: 1432-1084
    Keywords: Key words: Coronary vessels ; anomalies ; Coronary vessels ; CT ; Coronary vessels ; MR studies ; Coronary angiography ; Electron-beam CT
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. An anomalous origin of the left coronary artery arising from the pulmonary artery is a congenital malformation rarely described in adults. We report the case of a 65-year-old patient with this anomaly. Clinical presentation, imaging identification (coronary angiogram, MRI and electron-beam CT), surgical treatment and angiographic long-term follow-up are described.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003300050885
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 47
    Electronic Resource
    Electronic Resource
    Foreign-body granuloma of the kidney: CT, MR and pathologic correlation (1999)
    Springer
    European radiology 9 (1999), S. 1590-1592 
    Details
    ISSN: 1432-1084
    Keywords: Key words: Kidney ; Kidney neoplasms ; CT ; Fat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. The differential diagnosis of renal masses containing fatty foci is limited to a small number of well-defined tumors, angiomyolipoma being the most frequent. In recent years clear cell carcinomas with intratumoral fatty foci have been reported, due to either entrapment of local fat or to regressive adipose metaplasia. Demonstration of focal calcifications is a valuable sign, being relatively common in carcinomas while rare in more benign lesions. We report a case of a foreign-body granuloma of the kidney, containing both calcifications and foci of fat. The value of this case, in our opinion, is that it demonstrates that detection of the previously mentioned features in a renal mass does not necessarily imply a presumptive diagnosis of renal cell carcinoma.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003300050890
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 48
    Electronic Resource
    Electronic Resource
    Isolated penile metastasis from bladder carcinoma (1999)
    Springer
    European radiology 9 (1999), S. 1596-1598 
    Details
    ISSN: 1432-1084
    Keywords: Key words: Penile metastasis ; Corpus cavernosum ; Bladder cancer ; CT ; MRI
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. Metastases of the penis are uncommon, with only approximately 300 cases reported since 1870. In up to 70 % of patients, the primary tumour is located in the urogenital tract. Furthermore, isolated metastases of the penis are exceptionally rare. We report a case of solitary squamous cell metastasis of the penis presenting with painful swelling initially thought to be inflammatory in origin. The CT and MR imaging findings are presented with a short review of the literature.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003300050892
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 49
    Electronic Resource
    Electronic Resource
    Renal replacement lipomatosis: ultrasonography and computed tomography findings (1999)
    Springer
    European radiology 9 (1999), S. 1599-1601 
    Details
    ISSN: 1432-1084
    Keywords: Key words: Kidney ; US ; CT ; Lipomatosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. Replacement lipomatosis of the kidney is the result of severe atrophy or destruction of the renal parenchyma often caused by calculous disease with secondary marked proliferation of renal sinus, renal hilus, and perirenal fatty tissue. The diagnosis is difficult to establish with conventional radiographic methods. Although ultrasonography may show highly suggestive findings, computed tomography seems to be the most accurate method for demonstrating the distinctive features of replacement lipomatosis. Ultrasonographic and computed tomographic features in three cases of replacement lipomatosis of the kidney are reported.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003300050893
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 50
    Electronic Resource
    Electronic Resource
    Utility of CT scan for the diagnosis of chest wall tuberculosis (1999)
    Springer
    European radiology 9 (1999), S. 1638-1642 
    Details
    ISSN: 1432-1084
    Keywords: Key words: Bone ; Tuberculosis ; CT ; Rib ; Sternum ; Chest wall
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. The objective of this study was to determine the utility of CT scan findings for the diagnosis of chest wall tuberculosis, excluding the spine. We reviewed 15 patients (13 Africans and 2 Indians) with chest wall tuberculosis, retrospectively. The radiologic examination consisted of a plain X-ray and a CT scan of the chest for each patient. The site of disease was the rib in 13 patients or the body of the sternum in 2 patients. One rib was involved in 11 patients, 2 contiguous ribs (one site) in 2 patients, and bilateral disease (two sites) was observed in the remaining patient. The 14 rib sites involved the posterior arc or costovertebral joint in 11 cases, the anterior arc in 2 cases, and the anterior and middle arc in 1 case. The CT scan findings were an abscess (n = 14) or a soft tissue mass (n = 2), osteolytic lesions (n = 13), periosteal reaction (n = 10), and sequestrum (n = 14). Bone sclerosis was observed only in 3 cases of rib involvement. The association of a soft tissue abscess, an osteolytic lesion, and sequestrum, especially in immigrants to France, suggests chest wall tuberculosis on CT scan.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003300050900
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 51
    Electronic Resource
    Electronic Resource
    Cranial fasciitis in an adult: CT and MR imaging findings (1999)
    Springer
    European radiology 9 (1999), S. 1650-1652 
    Details
    ISSN: 1432-1084
    Keywords: Key words: Bone tumors ; Cranial fasciitis ; CT ; MR imaging
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. Cranial fasciitis is a rare bone lesion in childhood. We report the first case in an adult, with CT and MR imaging, and suggest some diagnostic keys.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003300050903
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 52
    Electronic Resource
    Electronic Resource
    Plexiform schwannoma of the foot (1999)
    Springer
    European radiology 9 (1999), S. 1653-1655 
    Details
    ISSN: 1432-1084
    Keywords: Key words: Plexiform schwannoma ; Soft tissue tumor ; Plexiform neurofibroma ; CT ; MR imaging
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. The present report describes a plexiform schwannoma involving the subcutis of the foot in an 8-year-old boy. Gross findings revealed thin fibrous septa in a multilobulated tumor that was partly separated into free body-like nodules in the subcutis. Preoperative CT and MRI failed to delineate this multinodular architecture or free bodies. This is a case presentation including the CT and MR findings associated with plexiform schwannoma.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003300050904
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 53
    Electronic Resource
    Electronic Resource
    CT diagnosis of delayed presentation of congenital diaphragmatic hernia simulating massive pleuropneumonia (1999)
    Springer
    European radiology 9 (1999), S. 1672-1674 
    Details
    ISSN: 1432-1084
    Keywords: Key words: Congenital diaphragmatic hernia ; Delayed presentation ; CT
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. A case of an 11-month-old infant with a delayed presentation of congenital diaphragmatic hernia is reported. Incarceration of the herniated colon caused a misleading appearance on the chest X-ray which was interpreted as massive pleuropneumonia. Computed tomography, performed because of continuing deterioration in the clinical condition, showed fluid-filled bowel loops in the chest and dilated bowel loops with air–fluid levels in the abdomen, suggesting the correct diagnosis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003300050907
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 54
    Electronic Resource
    Electronic Resource
    Imaging features of thalassemia (1999)
    Springer
    European radiology 9 (1999), S. 1804-1809 
    Details
    ISSN: 1432-1084
    Keywords: Key words: Thalassemia ; Extramedullary hematopoiesis ; MRI ; CT
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. Thalassemia is a kind of chronic, inherited, microcytic anemia characterized by defective hemoglobin synthesis and ineffective erythropoiesis. In all thalassemias clinical features that result from anemia, transfusional, and absorptive iron overload are similar but vary in severity. The radiographic features of β-thalassemia are due in large part to marrow hyperplasia. Markedly expanded marrow space lead to various skeletal manifestations including spine, skull, facial bones, and ribs. Extramedullary hematopoiesis (ExmH), hemosiderosis, and cholelithiasis are among the non-skeletal manifestations of thalassemia. The skeletal X-ray findings show characteristics of chronic overactivity of the marrow. In this article both skeletal and non-skeletal manifestations of thalassemia are discussed with an overview of X-ray findings, including MRI and CT findings.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003300050926
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 55
    Electronic Resource
    Electronic Resource
    Aberrant left brachiocephalic vein: CT imaging findings and embryologic correlation (1999)
    Springer
    European radiology 9 (1999), S. 1835-1839 
    Details
    ISSN: 1432-1084
    Keywords: Key words: Aberrant left brachiocephalic vein ; CT ; Embryonic
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. Computed tomography was utilized to evaluate aberrant left brachiocephalic vein (ALBCV), an infrequently discussed congenital vascular anomaly among Chinese people. Associated vascular variation and possible embryonic correlation are discussed. Since 1990, a total of 14 cases of ALBCV have been reported in patients receiving CT scan of chest, and was mainly an incidental diagnosis. One case was confirmed angiographically and two others were confirmed by magnetic resonance imaging. Emphasis was placed on the entry of the azygos vein into the superior vena cava (SVC), the length of the SVC, and the presence of other cardiovascular abnormalities. Of the 14 cases of ALBCV, the level of azygos vein entry was higher than the origin of the SVC in 7 cases: 4 were approximately the same level and 3 were lower. The average length of the SVC was approximately 5.6 cm shorter than that of the general population, which is approximately 7.0 cm. Three cases had associated vascular anomaly. Most cases of ALBCV had azygos vein drainage level higher than or equal to the origin of the SVC. Right-sided aorta is one of the causes giving rise to the ALBCV during embryonic development. The CT scan remains a definitive diagnostic modality for ALBCV.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003300050931
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 56
    Electronic Resource
    Electronic Resource
    Current value of double-contrast pharyngography and of computed tomography for the detection and for staging of hypopharyngeal, oropharyngeal and supraglottic tumors (1999)
    Springer
    European radiology 9 (1999), S. 1843-1850 
    Details
    ISSN: 1432-1084
    Keywords: Key words: Pharynx ; Larynx ; Neoplasms ; Radiography ; CT ; Deglutition disorders
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. In light of recent endoscopic techniques the current value of double-contrast pharyngography (DCP) and of CT for detection and staging of hypo-, oropharyngeal, and supraglottic tumors is evaluated. The DCP of 151 patients and CT obtained from 99 of these patients were retrospectively analyzed in a double-blinded manner. We used a standard protocol which comprised all relevant anatomical subregions. Results were compared with direct microlaryngoscopy (DL), indirect laryngoscopy (IL), and post-operative histopathological findings. Sensitivity and specificity of DCP was 75.0 % and 86.7 %, respectively. The DCP and IL techniques together yielded a higher sensitivity (96.7 %) than each method separately. Sensitivity and specificity of CT was 87.5 and 100 %, respectively. In 74.7 % CT provided correct staging. Subregional analysis revealed that the results of DCP and CT depend highly on the localization of the tumor. Our results indicate that DCP represents an important screening method for diagnosing hypo-, oropharyngeal, and supraglottic tumors to complete IL and DL. We show that CT is a reliable method for preoperative staging, although small superficial tumors may occasionally be missed by this method.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003300050933
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 57
    Electronic Resource
    Electronic Resource
    Evaluation of the middle and inner ear structures: comparison of hybrid rendering, virtual endoscopy and axial 2D source images (1999)
    Springer
    European radiology 9 (1999), S. 1851-1858 
    Details
    ISSN: 1432-1084
    Keywords: Key words: Temporal bone ; CT ; Ear ; middle ; Ear ; labyrinth ; Three-dimensional CT ; hybrid rendering ; Virtual endoscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. Recent developments in 3D reconstructions can enhance the quality and diagnostic value of axial 2D image data sets with direct benefits for clinical practice. To show the possible advantages of a hybrid rendering method [color-coded 3D shaded-surface display (SSD)- and volume rendering method] with the possibility of virtual endoscopy we have specifically highlighted the use in relation to the middle and inner ear structures. We examined 12 patients with both normal findings and postoperative changes, using image data sets from high-resolution spiral computed tomography (HRSCT). The middle and inner ear was segmented using an interactive threshold interval density volume-growing method and visualized with a color-coded SSD rendering method. The temporal bone was visualized using a transparent volume rendering method. The 3D- and virtual reconstructions were compared with the axial 2D source images. The evaluated middle and inner ear structures could be seen in their complete form and correct topographical relationship, and the 3D- and virtual reconstructions indicated an improved representation and spatial orientation of these structures. A hybrid and virtual endoscopic method could add information and improve the value of imaging in the diagnosis and management of patients with middle or inner ear diseases making the understanding and interpretation of axial 2D CT image data sets easier. The introduction of an improved rendering algorithm aids radiological diagnostics, medical education, surgical planning, surgical training, and postoperative assessment.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003300050934
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 58
    Electronic Resource
    Electronic Resource
    A case of totally cystic fibroepithelial polyp of the renal pelvis (1999)
    Springer
    European radiology 9 (1999), S. 1873-1875 
    Details
    ISSN: 1432-1084
    Keywords: Key words: Fibroepithelial polyp ; Kidney cyst ; CT ; Ultrasound
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. Fibroepithelial polyps are the most frequently observed mesenchymal tumors of the renal pelvis. We report on one case of fibroepithelial polyp of the renal pelvis with unusual CT findings of totally cystic structure with septations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003300050938
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 59
    Electronic Resource
    Electronic Resource
    Intraluminal duodenal diverticulum: radiological and endoscopic ultrasonography findings of an unusual cause of acute pancreatitis (1999)
    Springer
    European radiology 9 (1999), S. 1898-1900 
    Details
    ISSN: 1432-1084
    Keywords: Key words: Duodenal cyst ; CT ; Endoscopic ultrasonography ; Duodenal diverticulum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. Intraluminal duodenal diverticulum is a rare congenital web of membrane which may be symptomatic when it becomes distended. This report describes a case revealed by presenting as an acute pancreatitis. The radiological findings are reported. The findings at CT, upper gastro-intestinal series, endoscopic ultrasound and endoscopic retrograde cholangiopancreatography are described and differential diagnostic features from choledochocele and duodenal duplication are discussed. By endoscopic ultrasonography, observation of a thin wall, without different layers such as choledochocele or duodenal duplication, may be useful for diagnosis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003300050944
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 60
    Electronic Resource
    Electronic Resource
    Evaluation of the carotid and vertebral arteries: comparison of 3D SCTA and IA-DSA-work in progress (1999)
    Springer
    European radiology 9 (1999), S. 105-112 
    Details
    ISSN: 1432-1084
    Keywords: Key words: Computed tomography (CT) ; spiral technology ; Carotid arteries ; CT ; Vertebral arteries ; CT ; Computed tomography (CT) ; image processing ; Computed tomography (CT) ; threedimensional visualization ; Computed tomography (CT) ; comparative studies
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. Objective: The purpose of this study was to develop a method for three-dimensional (3D) visualization of the whole vascular system of the carotid and vertebral arteries using spiral computed tomographic angiography (SCTA), that allows accurate, qualitative and quantitative evaluation, of anatomical abnormalities, including detection of additional lesions, and estimation of degree of stenosis. Materials and methods: Fifteen patients with anatomical and pathological abnormalities of the arterial vascular system detected by color-coded duplex ultrasound were studied using intraarterial digital subtraction angiography (IA-DSA) with aortic arch injection, and SCTA. The carotid and vertebral arteries were segmented using an interactive threshold interval density volume-growing method and visualized with a color-coded shaded-surface display (SSD) rendering method. The adjacent bone structures were visualized using a transparent volume rendering method. Results: In all cases, the entire volume of the vascular system of the carotid and vertebral arteries could be visualized on SCTA, and the anatomical and pathological abnormalities on 3D SCTA correlated well with that seen on IA-DSA. Conclusion: Results of 3D SCTA had a high degree of correlation with results of IA-DSA in the evaluation of the vascular system of the carotid and vertebral arteries. The 3D SCTA with a subsecond spiral CT scanner is useful for the visualization of anatomical and pathological abnormalities in the circulation in the carotid and vertebral arteries and offer a promising minimally invasive alternative compared with other diagnostic procedures.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003300050639
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 61
    Electronic Resource
    Electronic Resource
    MR imaging and CT of the female pelvis: radiologic–pathologic correlation (1999)
    Springer
    European radiology 9 (1999), S. 3-15 
    Details
    ISSN: 1432-1084
    Keywords: Key words: Cervical carcinoma ; Endometrial carcinoma ; Leiomyoma ; Adenomyosis ; Benign adnexal masses ; Ovarian carcinoma ; Female pelvis ; MR imaging ; CT
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. For imaging of the female pelvis, transvaginal ultrasound is the method of choice. Magnetic resonance imaging and CT provide important additional information in various disorders. Magnetic resonance imaging is superior to CT in diagnosing benign and malignant disorders of the uterus. The same holds for the characterization of adnexal masses, where MR imaging reliably differentiates dermoids, ovarian fibromas, and most endometriomas. Differentiation of other benign and malignant ovarian tumors by CT and MR imaging is based on identical morphological criteria; no superiority of MR imaging over CT has been established. Computed tomography is still the preferred imaging modality for staging ovarian cancer. A thorough knowledge of the pathomorphological changes associated with the different disorders of the female pelvis not only helps to choose the proper imaging modality and examination protocol, but also improves image interpretation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003300050620
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 62
    Electronic Resource
    Electronic Resource
    Pulmonary lymphangioleiomyomatosis: high-resolution CT findings (1999)
    Springer
    European radiology 9 (1999), S. 49-54 
    Details
    ISSN: 1432-1084
    Keywords: Key words: Lymphangioleiomyomatosis ; Lymphangiomyomatosis ; CT ; Lung ; CT ; comparative studies
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. Lymphangioleiomyomatosis (LAM) of the lung is a very rare disease. There are obvious discrepancies in the literature concerning the appearance of LAM on CT scans of the lung. This study adds the imaging findings of 11 patients and demonstrates how the imaging findings changed over time in four patients. Twenty-two CT examinations, and radiographs that had been obtained close to the CT examinations, of 11 patients with LAM confirmed by open lung biopsy were retrospectively evaluated with particular attention to the size of cystic lesions and wall thickness. Furthermore the CT scans were analysed for the type of pulmonary infiltration process and its distribution, presence or absence of pleural effusion, pneumothorax and lymph node enlargement. Clinical and CT follow-up studies were available in four patients. The CT scans revealed an increase in the interstitial pattern in all patients. Architectural distortion was seen in two patients and cystic lesions were present in all. The size of the cysts varied from small lesions to bullous emphysema. The cystic lesions revealed a wall thickness up to 2 mm but a wall was not perceptible in all. Pneumothorax was seen in only two patients; pleural effusion was seen in two patients. CT examination of patients with LAM reveals neither a uniform nor a pathognomonic appearance. In the early stages of LAM or in cases with interstitial changes the differential diagnosis of centrilobular emphysema or idiopathic pulmonary fibrosis seems to be more difficult than most authors believe.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003300050626
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 63
    Electronic Resource
    Electronic Resource
    Assessment of gastric cancer: value of breathhold technique and two-phase spiral CT (1999)
    Springer
    European radiology 9 (1999), S. 68-72 
    Details
    ISSN: 1432-1084
    Keywords: Key words: Gastrointestinal tract ; Neoplasms ; Stomach ; Neoplasms ; CT
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. The purpose of this study was to evaluate the capabilities of subsecond spiral CT in detecting and staging of gastric cancer. Our study included 40 patients with endoscopically detected gastric carcinomas. Two-phase spiral CT was performed within one breathhold each. Distension of the stomach was achieved by intravenous application of scopolamine and drinking of 500 ml water. After bolus injection of contrast medium, scanning was performed in the arterial and venous phase. Gastric tumour extention and lymph node involvement was assessed. Gastric cancer was detected in 39 of 40 cases (sensitivity 97.5 %). Location of the tumour was correctly assessed in all cases. In 31 of the 39 cases (79.4 %) CT staging was accordant with pathological staging. One hundred two (70 %) of 145 nodes infiltrated by tumour tissue were detected and 144 (42.8 %) of 336 nodes free of metastatic involvement were found. The predictive values of positive and negative results for the detection of lymph node metastases were 67.1 and 75 %, respectively. Spiral CT is recommended for staging of gastric cancer.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003300050630
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 64
    Electronic Resource
    Electronic Resource
    Extramedullary hematopoiesis related to Paget's disease (1999)
    Springer
    European radiology 9 (1999), S. 205-207 
    Details
    ISSN: 1432-1084
    Keywords: Key words: Extramedullary hematopoiesis ; Paget's disease ; Thorax ; Neoplasm ; CT ; MR imaging
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. Extramedullary hematopoiesis usually occurs in hematological diseases but may also be found as an uncommon complication of Paget's disease, probably due to bone effraction mechanism. We present a case of intrathoracic extramedullary hematopoiesis related to Paget's disease. To our knowledge, this is the seventh case reported in the literature. We describe and correlate the conventional X-ray, CT, MR imaging, and cytological findings.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003300050656
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 65
    Electronic Resource
    Electronic Resource
    Exogenous lipoid pneumonia: high-resolution CT findings (1999)
    Springer
    European radiology 9 (1999), S. 287-291 
    Details
    ISSN: 1432-1084
    Keywords: Key words: Lung ; CT ; Pneumonia ; Aspiration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. The aim of this study was to assess high-resolution computed tomography (HRCT) findings of exogenous lipoid pneumonia. High-resolution computed tomography was obtained in 25 patients with proven exogenous lipoid pneumonia resulting from aspiration of squalene (derived from shark liver oil). Diagnosis was based on biopsy (n = 9), bronchoalveolar lavage (n = 8), or sputum cytology and clinical findings (n = 8). The clinical history of taking squalene was confirmed in all patients. The CT findings were classified into three patterns: diffuse ground-glass opacity, consolidation, and interstitial abnormalities. Distribution of the abnormalities, duration of taking squalene, predisposing factors for aspiration, and route of administration were analyzed. Ten patients showed diffuse ground-glass opacity pattern. Seven of 10 patients had predisposing conditions such as unconsciousness, pharyngeal dysmotility, or motor disturbances, and 6 patients had a recent history of taking large amount of squalene through nasal route. Seven patients who had consolidation pattern had a history of taking squalene for several months and did not have any predisposing factor. All of the 5 patients who had a pattern of interstitial abnormalities had a history of taking squalene longer than 1 year and showed segmental distribution of interstitial thickening with interposing ground-glass opacities. Three patients simultaneously had two different patterns at different lobes of the lung. The HRCT findings of lipoid pneumonia are ground-glass opacities, consolidation, and interstitial abnormalities. These HRCT findings with appropriate inquiries could be useful for diagnosis of exogeneous lipoid pneumonia.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003300050669
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 66
    Electronic Resource
    Electronic Resource
    Orientation of trabecular bone in human vertebrae assessed by MRI (1999)
    Springer
    European radiology 9 (1999), S. 643-647 
    Details
    ISSN: 1432-1084
    Keywords: Key words: MRI ; CT ; Lumbar vertebrae ; Osteoporosis ; Trabeculae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. The aim of the study was to assess the relative number of bone trabeculae in different orientations by using magnetic inhomogeneity measurements by MR imaging. Twelve defatted human vertebrae (L2–L4) were studied by MR imaging and CT. In the MR measurements the reversible transaxial decay rate, R2', was determined using the GESFIDE sequence. The relative contribution to R2' of bone trabeculae oriented along the x (R2'x), y (R2'y) and z (R2'y) axes was assessed, by rotation of the specimen in the magnetic field. The results were validated by CT measurements of trabecular structure at a resolution of 0.2 × 0.2 × 1 mm, using custom-made software. R2' ranged from 4.9 to 32 s–1. After separation, theoretical R2'x ranged from 2.3 to 10.7 s–1, R2'y ranged from 2.6 to 14.0 s–1 and R2'z ranged from 4.7 to 17.9 s–1. The number of bone trabeculae per millimeter identified in axial CT images ranged from 0.15 to 0.38 and from 0.039 to 0.22 per millimeter in sagittal images. The difference between axial and sagittal CT images was statistically significant and due to anisotropic voxels. The correlation between R2' and the number of bone trabeculae per millimeter was statistically significant (r = 0.83, p 〈 0.001 for x orientation; r = 0.63 for y orientation, and r = 0.59 for z orientation, p 〈 0.05 for both). The relative amount of bone trabeculae in different orientations can be assessed in vitro using R2' measurements.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003300050725
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 67
    Electronic Resource
    Electronic Resource
    Appendicular involvement in perforated sigmoid disease: US and CT findings (1999)
    Springer
    European radiology 9 (1999), S. 697-700 
    Details
    ISSN: 1432-1084
    Keywords: Key words: Appendicitis ; Diverticulitis ; US ; CT ; Pitfalls
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. We describe four patients in whom ultrasound (US) and/or computed tomography (CT) demonstrated a thickened appendix, secondarily enlarged due to perforated sigmoid diverticulitis (n = 2) or carcinoma (n = 2). The underlying pathology was correctly recognized in all cases. Secondary thickening of the appendix due to perforated sigmoid disease provides a potential pitfall mainly on US and may lead to an incorrect diagnosis and thus to unnecessary surgery or a wrong surgical intervention. Although US alone is enough to diagnose periappendicitis and sigmoid disease, combined use of US and CT may improve assessment of its origin and extension.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003300050735
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 68
    Electronic Resource
    Electronic Resource
    A new method to measure vertebral rotation from CT scans (1999)
    Springer
    European spine journal 8 (1999), S. 261-265 
    Details
    ISSN: 1432-0932
    Keywords: Key words Vertebral rotation ; Idiopathic scoliosis ; CT ; Method
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract CT measurement methods have good reliability for idiopathic scoliosis transverse plane deformity evaluation. However, because of application difficulties and variations in how these methods are applied, more sensitive methods are needed. This paper presents a new method for measurement of vertebral rotation from tomographic scans. First, the method was subject to clinical, intra-observer and inter-observer analysis. Twenty-three patients with adolescent idiopathic scoliosis were studied to test the clinical reliability of this method. There were no statistical differences between the results of the new method and Ho’s method (P = 0.3380) in the clinical study. Intra-observer and inter-observer analysis showed that this method was reliable. An experimental study was then conducted to show the confidence limits of our new method, which were found to be ± 1.6°, and there was no significant difference between the mean rotation value obtained from CT scans using our new method and that obtained using the mechanical method. These results suggest that our new method is a simple, practical and reliable method for measurement of vertebral rotation from CT scans.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s005860050170
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 69
    Electronic Resource
    Electronic Resource
    Cervical synovial cysts: case report and review of the literature (1999)
    Springer
    European spine journal 8 (1999), S. 232-237 
    Details
    ISSN: 1432-0932
    Keywords: Key words Synovial cyst ; CT ; MRI ; Surgical treatment
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The authors describe the case of a 58-year-old man with a 6-month history of severe myelopathy. CT scan and MRI of the spine revealed a cystic formation, measuring about 1 cm in diameter, at C7-T1 at a right posterolateral site at the level of the articular facet. At operation the mass appeared to originate from the ligamentum flavum at the level of the articular facet and was in contact with the dura mater. Once the mass had been removed, there was a significant amelioration of the patient’s symptoms. As previously suspected, histological aspect was synovial cyst. Cervical synovial cysts are extremely rare and, as far as we know, only 22 cases have so far been described in the literature. Diagnostic radiological investigations used were CT scan and MRI. At CT scan the most important diagnostic findings are a posterolateral juxtafacet location of the mass, egg-shell calcifications on the wall of the cyst, and air inside the cyst. At MRI the contents of the cyst are iso/hypointense on T1- and hyperintense on T2-weighted images. There may also be a hypointense rim on T2-weighted images, which enhances after i.v. administration of gadolinium. Surgical treatment consists of removal of the mass. Fixation of the vertebral segments involved is not always necessary.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s005860050164
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 70
    Electronic Resource
    Electronic Resource
    Tumors of peripheral nerves: correlation of symptoms, clinical signs, imaging features, and histologic diagnosis (1999)
    Springer
    Skeletal radiology 28 (1999), S. 183-188 
    Details
    ISSN: 1432-2161
    Keywords: Key words Malignant peripheral nerve sheath tumor ; Schwannoma ; Neurofibroma ; CT ; MRI
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  Objective. To distinguish between benign and malignant tumors in the peripheral nerves. Design and patients. The clinical, imaging and histologic findings of 99 benign and 16 malignant tumors in the peripheral nerves were reviewed retrospectively. Results. Preoperative motor weakness was observed in only six of 99 benign tumors and was mild, while slight to severe motor weakness was present in 15 of 16 malignant lesions. Pain at rest was present in five of 99 benign tumors and in 15 of 16 malignant tumors. All benign lesions showed a smooth tumoral margin, while half the malignant lesions showed an invasive margin on CT or MRI. Thirteen of 28 benign lesions on CT and nine of 23 on MRI showed round to geographic central enhancement, but this pattern was not seen in malignant lesions. Conclusion. Absence of severe motor weakness and a central enhancement pattern strongly suggest a benign nature, while severe rest pain and invasive tumor margin suggest malignant lesions in peripheral nerve tumors.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002560050498
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 71
    Electronic Resource
    Electronic Resource
    The radiological findings in chronic expanding hematoma (1999)
    Springer
    Skeletal radiology 28 (1999), S. 396-401 
    Details
    ISSN: 1432-2161
    Keywords: Key words Chronic expanding hematoma ; Ancient hematoma ; CT ; MRI ; Soft tissue mass
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  Objective. To identify the characteristic MRI findings of chronic expanding hematoma correlated with the pathology. Design and patients. Three patients who had a chronic expanding hematoma involving the musculoskeletal system were reviewed retrospectively. Results and conclusion. Huge soft tissue masses suggestive of malignancy with destruction of the bony structure were revealed on radiography and computed tomography. MRI showed the masses to exhibit heterogeneous signal intensity on both T1- and T2-weighted images with a peripheral rim of low signal intensity, reflecting the central zones of fluid collection due to fresh and altered blood with a wall of collagenous fibrous tissue. These MRI findings were seen in all three patients and are considered to be characteristic; they assist in differentiation from neoplasm in consideration of the history of trauma or surgery.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002560050536
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 72
    Electronic Resource
    Electronic Resource
    Post-Paget telangiectatic osteosarcoma of the skull (1999)
    Springer
    Skeletal radiology 28 (1999), S. 470-472 
    Details
    ISSN: 1432-2161
    Keywords: Key words Telangiectatic osteosarcoma ; CT ; Paget’s disease ; skull
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  Sarcomatous transformation is the most dreaded complication of Paget’s disease. We report on a case of post-Paget telangiectatic osteosarcoma of the skull, a variant of osteogenic osteosarcoma, in a 79-year-old woman. We discuss the radiological pattern in relationship to the differential diagnosis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002560050549
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 73
    Electronic Resource
    Electronic Resource
    Malignant degeneration of an osteochondroma with unusual intra-bursal invasion (1999)
    Springer
    Skeletal radiology 28 (1999), S. 540-543 
    Details
    ISSN: 1432-2161
    Keywords: Key words Chondrosarcoma ; Osteochondroma ; Bursa ; CT ; MRI
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  Multiple hereditary osteochondromatosis is an uncommon autosomal dominant condition in which patients are predisposed to the development of chondrosarcoma. We report a case of a patient who developed a secondary low-grade chondrosarcoma in this setting. The tumor was associated with an unusual multinodular invasive growth pattern into a pre-existing bursa that was present overlying the osteochondroma.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002560050560
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 74
    Electronic Resource
    Electronic Resource
    Osteolytic extra-axial sacral myxopapillary ependymoma (1999)
    Springer
    Skeletal radiology 28 (1999), S. 584-589 
    Details
    ISSN: 1432-2161
    Keywords: Key words Myxopapillary ependymoma ; Sacrum ; Osteolysis ; X ray ; CT ; MRI
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  The authors report an unusual case of sacral osteolytic myxopapillary ependymoma treated with curettage and radiotherapy. There is no evidence of recurrence 8 years after treatment. A review of the literature is presented on sacral ependymomas presenting with an osteolytic radiographic appearance (24 cases in 18 reports). The differential diagnosis with other sacral neoplasms is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002560050624
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 75
    Electronic Resource
    Electronic Resource
    Die Diagnostik der Stadien T1 und T2 des Larynxkarzinoms mit dem Mehrschicht-Spiral-CT (1999)
    Springer
    Der Radiologe 39 (1999), S. 939-942 
    Details
    ISSN: 1432-2102
    Keywords: Schlüsselwörter Multidetektor ; CT ; MSCT ; Larynx ; Tumoren ; Multiplanare Rekonstruktion ; Key words Computer tomography ; Larynx Head and neck tumors
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary The purpose of the study was to preoperatively investigate small laryngeal carcinomas using multi-slice spiral CT (MSCT) and subsequent multiplanar reconstructions (MPR) and to compare the results to the detailed spread found at surgery and histology. Nine patients with small (T1, T2) laryngeal cancer were investigated on a MSCT scanner (Siemens plus 4 Volume Zoom, Siemens). A 4×1 mm collimation, 120 kV, 200 mAs and a 0.5 seconds rotation time were used, allowing a coverage of the entire larynx in approximately 10 seconds within a single breathhold. Multiplanar reconstruction’s (MPR) in sagittal and coronal plane were reconstructed in all patients and rated in consensus reading. In 8 of nine patients, the glottic spread was detected by MSCT, in one cause of a supraglottic tumor a glottic invasion was excluded. The infiltration of the anterior commissure, the infiltration into the subglottic space and the extension into the hypopharynx was correctly assessed in all patients. MSCT was not able to predict infiltration of the arythnoids in two patients. The use of multi-slice spiral CT for the preoperative assessment of small laryngeal tumors shows great promise. The detection or exclusion of subtle spread of these tumors into the supra- or subglottic space and along the glottic level was possible with high accuracy. As the examination time is short, artifacts are rare and multiplanar reconstructions gain in clinical importance.
    Notes: Zusammenfassung Zielsetzung war, kleine Larynxkarzinome mit der Mehrschicht-Spiral-CT (MSCT) und nachfolgenden multiplanaren Rekonstruktionen (MPR) präoperativ zu untersuchen. 9 Patienten mit histologisch gesichertem Larynxkarzinom der Stadien T1 und T2 wurden untersucht. Alle Untersuchungen wurden an einem Mehrschicht-Spiral-CT (Somatom Plus 4 Volume Zoom, Siemens, Forchheim) durchgeführt. Die Abtastung erfolgte mit 4×1 mm Kollimation, 120 kV, 200 mAs bei einer Rotationszeit der Röhre von 0,5 s. Die Beurteilung einer Infiltration erfolgte durch die Bewertung aller drei Ebenen. Das glottische Wachstum entlang der Stimmlippen konnte in 8 von 9 Patienten richtig-positiv nachgewiesen werden. In einem Fall eines supraglottischen Tumors konnte eine glottische Infiltration ausgeschlossen werden. Die Infiltration der vorderen Kommissur, die Infiltration in den subglottischen Raum und die Ausdehnung in den Hypopharynx konnte ebenfalls sicher nachgewiesen bzw. ausgeschlossen werden. Die Infiltration der Aryknorpel konnte in 7 von 9 Fällen korrekt durch die MSCT vorhergesagt werden. Der Einsatz der Mehrschicht-Spiral-CT bei kleinen Larynxkarzinomen zeigt eine hohe Genauigkeit zum Nachweis bzw. zum Ausschluß von Infiltrationen sowohl in der glottischen Ebene, wie auch in den supra- oder subglottischen Raum. Bedingt durch die kurze Untersuchungszeit sind Bewegungsartefakte selten. Multiplanare Rekonstruktionen (MPR) sind klinisch sehr wertvoll.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001170050585
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 76
    Electronic Resource
    Electronic Resource
    Präoperatives Staging des Kolorektalen Karzinoms (1999)
    Springer
    Der Radiologe 39 (1999), S. 562-567 
    Details
    ISSN: 1432-2102
    Keywords: Schlüsselwörter Kolorektales Karzinom ; Präoperatives Staging ; Endosonographie ; CT ; MRT ; Key words Colorectal cancer ; Preoperative staging ; Endosonography ; Computed tomography ; Magnetic resonance imaging
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary Carcinoma of the colorectum is one of the most frequent neoplasias, with an incidence of 40 in 100 000. For the effective use of new, differentiated, less invasive treatment options, exact preoperative staging of the tumor is essential. The introduction of endosonography in rectal tumor staging allows for exact differentiation of the rectal wall layers and thus of tumor stages 1–3 with median accuracy of 89%. Magnetic resonance imaging of the rectum, especially in double-contrast technique, can also be employed in high and stenosing tumors and leads to an average accuracy of 85% for the stages 1–4. Computed tomography is the method of choice in screening for metastases. In lymph node staging, all modalities show only moderate accuracy around 75%.
    Notes: Zusammenfassung Das kolorektale Karzinom stellt mit einer Inzidenz von 40 auf 100000 eine der häufigsten Neoplasien dar. Für den effektiven Einsatz neuer differenzierter Therapien mit geringerer Invasivität ist ein exaktes präoperatives Staging erforderlich. Die Einführung der Endosonographie im Tumorstaging erlaubt eine exakte Differenzierung der Wandschichten und damit der Tumorstadien 1–3 mit einer mittleren Genauigkeit von 89%, die Magnetresonanztomographie des Rektums, insbesondere in Doppelkontrasttechnik, kann auch bei hohen und stenosierenden Tumoren angewendet werden, bei einer Genauigkeit von durchschnittlich 85% für die Stadien 1–4. Die Computertomographie ist Methode der Wahl im Metastasenscreening. Im Lymphknotenstaging zeigen alle Modalitäten nur bescheidene Genauigkeiten um 75%.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001170050551
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 77
    Electronic Resource
    Electronic Resource
    Bildgebende Diagnostik im Staging gynäkologischer Karzinome (1999)
    Springer
    Der Radiologe 39 (1999), S. 610-618 
    Details
    ISSN: 1432-2102
    Keywords: Schlüsselwörter Staging ; Gynäkologische Karzinome ; Schnittbildverfahren ; Computertomographie (CT) ; Magnetresonanztomographie (MRT) ; Sonographie (US) ; Key words Staging ; Gynecologic neoplasm ; Cross-sectional imaging modalities ; CT ; MRI ; Sonography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary The prognosis in patients with gynecologic cancers depends not only on the stage but also on a wide spectrum of other findings. Cross-sectional imaging modalities, including sonography, CT and MRI, have increasingly been used for optimal treatment planning in gynecologic cancers. Their staging criteria are based on the well-established FIGO staging system. CT and MRI compete with sonography, which plays a pivotal role in the evaluation of the female pelvis. This paper reviews the role of sonography, CT and MRI in the staging of gynecologic malignancies. It puts the emphasis on MRI, which has been established as imaging modality of choice in the preoperative evaluation of cervical and endometrial cancer, and which seems slightly superior to CT in the staging of ovarian cancer.
    Notes: Zusammenfassung Die Prognose gynäkologischer Karzinome hängt nicht nur vom Stadium, sondern von einem breiten Spektrum anerkannter Kriterien ab. Zur optimierten Therapieplanung werden heute zunehmend die Schnittbildverfahren Sonographie, CT und MRT für die morphologische Charakterisierung der Veränderungen eingesetzt. Die Stagingkriterien der bildgebenden Verfahren orientieren sich an der anerkannten FIGO-Klasssifikation. CT und MRT konkurrieren mit der Sonographie, die als anerkannte und weitverbreitete Modalität in der Gynäkologie etabliert ist. In dieser Übersichtsarbeit werden die Schnittbildverfahren Sonographie, CT und MRT im Staging gynäkologischer Karzinome einander gegenübergestellt. Der Schwerpunkt der Ausführungen liegt dabei auf der MRT, die als Methode der Wahl zum Staging des Zervix- und Endometriumkarzinoms gilt, beim Ovarialkarzinom der CT ebenbürtig und in manchen Fragestellung tendentiell überlegen ist.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001170050557
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 78
    Electronic Resource
    Electronic Resource
    Novel Technology for Detection of Genomic and Transcriptional Alterations in Pancreatic Cancer (1999)
    Springer
    Annals of oncology 10 (1999), S. 64-68 
    Details
    ISSN: 1569-8041
    Keywords: chromosomal aberrations ; gene expression ; oncogenes ; pancreatic cancer ; tumor suppressor genes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Aim: The present review summarizes our strategies aimed at identifying and characterizing genetic alterations occuring at the transcriptional and chromosomal level in pancreatic cancer. Methods: To study transcriptional alterations we have used a number of techniques including modified versions of differential hybridizations and cDNA-RDA (representational difference analysis). Comparative genomic hybridization (CGH) was used to study chromosomal aberrations occuring in pancreatic cancer tissues. Results: The study of transcriptional alterations led to the identification of more than 500 genes with differential expression in pancreatic cancer. The sum of these alterations represented the first expression profile characteristic for pancreatic tumors. The CGH analysis allowed the identification of a number of chromosomal regions containing putative tumor suppressor genes or oncogenes. These regions are presently being characterized at the molecular level. In a first approach the myb-oncogene was identified as the relevant oncogene of an amplification on 6q occurring in up to 10% of pancreatic cancer patients. Conclusions: Genes isolated in both approaches represent potential new disease genes for pancreatic cancer and are at present being characterized by individual or serial analysis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008392904359
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 79
    Electronic Resource
    Electronic Resource
    CT-Based Interstitial HDR Brachytherapy (1999)
    Springer
    Strahlentherapie und Onkologie 175 (1999), S. 419-427 
    Details
    ISSN: 1439-099X
    Keywords: Key Words: Interstitial HDR brachytherapy ; CT ; Conformal treatment ; Schlüsselwörter: Interstitielle HDR-Brachytherapie ; CT ; Konformale Therapie
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Ziel: Entwicklung, Anwendung und Evaluierung einer CT-gesteuerten Implantationstechnik und einer vollständig CT-gestützten Behandlungsplanung für Brachytherapie. Material und Methoden: Es wurde ein Brachytherapieverfahren, basierend auf einer CT-gesteuerten Implantationstechnik und CT-gestützter Bestrahlungsplanung, entwickelt und klinisch evaluiert. Für diese Zielsetzung wurde ein Softwaresystem (PROMETHEUS) zur 3D-Rekonstruktion der Applikatoren und der individuellen anatomischen Verhältnisse unter ausschließlicher Verwendung von CT-Schnittbildern entwickelt. Hierzu wurde ein Interface für das Nucletron-PLATO-BPS-Planungssystem zur Optimierung und Berechnung der Dosisverteilung realisiert. Das Zielvolumen (PTV) wird definiert und zur Optimierung der dreidimensionalen Dosisverteilung verwendet. Die Analyse der Dosisverteilung mittels Dosisvolumenhistogrammen (COIN) ermöglicht eine klinische Bewertung des Brachytherapieverfahrens. Die CT-gesteuerte Katheterimplantation und die CT-gestützte Bestrahlungsplanung zur Behandlung unterschiedlicher Tumoren in verschiedenen anatomischen Regionen wurden bei 197 Patienten in dem Zeitraum 1996 bis 1997 durchgeführt. Ergebnisse: Die Genauigkeit der CT-Rekonstruktion wurde mit Hilfe eines Brachytherapie-QA-Phantoms und eines Implantats mit zwölf Nadeln überprüft. Die Ergebnisse wurden mit denen der konventionellen Rekonstruktion aus Röntgenaufnahmen verglichen. Beide Methoden ergaben vergleichbare Ergebnisse in Hinblick auf die Rekonstruktionsgenauigkeit. Die CT-gestützte Rekonstruktion war jedoch schneller. In der klinischen Anwendung wurden Tumorvolumina zwischen 5,1 und 2 741 cm3 behandelt. Die Auswertung der Bestrahlungspläne ergab einen geringfügig signifikant geringeren COIN-Wert für die Knochenimplantate, aber keine Unterschiede hinsichtlich des Tumorvolumens. Schlußfolgerung: Die entwickelte Offenbacher Methode hat sich in der klinischen Routine zur Durchführung einer CT-gestützten Brachytherapie in unterschiedlichen Tumorlokalisationen bewährt. Unsere CT-gesteuerte Implantationstechnik zusammen mit dem CT-gestützten Planungssystem ermöglicht uns eine konformale Brachytherapiebehandlung.
    Notes: Purpose: Development, application and evaluation of a CT-guided implantation technique and a fully CT-based treatment planning procedure for brachytherapy. Methods and Materials: A brachytherapy procedure based on CT-guided implantation technique and CT-based treatment planning has been developed and clinical evaluated. For this purpose a software system (PROMETHEUS) for the 3D reconstruction of brachytherapy catheters and patient anatomy using only CT scans has been developed. An interface for the Nucletron PLATO BPS treatment planning system for optimization and calculation of dose distribution has been devised. The planning target volume(s) are defined as sets of points using contouring tools and are used for optimization of the 3D dose distribution. Dose-volume histogram based analysis of the dose distribution (COIN analysis) enables a clinically realistic evaluation of the brachytherapy application to be made. The CT-guided implantation of catheters and the CT-based treatment planning procedure has been performed for interstitial brachytherapy and for different tumor sites in 197 patients between 1996 and 1997. Results: The accuracy of the CT reconstruction was tested using first a quality assurance phantom and second, a simulated interstitial implant of 12 needles. These were compared with the results of reconstruction using radiographs. Both methods gave comparable results with regard to accuracy, but the CT based reconstruction was faster. Clinical feasibility was proved in pre-irradiated recurrences of brain tumors, in pretreated recurrences or metastatic disease, and in breast carcinomas. The tumor volumes treated were in the range 5.1 to 2,741 cm3. Analysis of implant quality showed a slightly significant lower COIN value for the bone implants, but no differences with respect to the planning target volume. Conclusions: The Offenbach system, incorporating the PROMETHEUS software for interstitial HDR brachytherapy has proved to be extremely valuable in routine clinical practice for many tumor sites. Our CT-guided implantation technique together with a fully CT-based planning system has enabled conformal brachytherapy treatment to become routine.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s000660050031
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 80
    Electronic Resource
    Electronic Resource
    Increased peroxisomal fatty acid β-oxidation and enhanced expression of peroxisome proliferator-activated receptor-α in diabetic rat liver (1999)
    Springer
    Molecular and cellular biochemistry 194 (1999), S. 227-234 
    Details
    ISSN: 1573-4919
    Keywords: microbodies ; diabetes mellitus ; steroid hormone receptor ; β-oxidation ; gene expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract To determine whether the increased fatty acid β-oxidation in the peroxisomes of diabetic rat liver is mediated by a common peroxisome proliferation mechanism, we measured the activation of long-chain (LC) and very long chain (VLC) fatty acids catalyzed by palmitoyl CoA ligase (PAL) and lignoceryl CoA ligase and oxidation of LC (palmitic acid) and VLC (lignoceric acid) fatty acids by isotopic methods. Immunoblot analysis of acyl-CoA oxidase (ACO), and Northern blot analysis of peroxisome proliferator-activated receptor (PPAR-α), ACO, and PAL were also performed. The PAL activity increased in peroxisomes and mitochondria from the liver of diabetic rats by 2.6-fold and 2.1-fold, respectively. The lignoceroyl-CoA ligase activity increased by 2.6-fold in diabetic peroxisomes. Palmitic acid oxidation increased in the diabetic peroxisomes and mitochondria by 2.5-fold and 2.7-fold, respectively, while lignoceric acid oxidation increased by 2.0-fold in the peroxisomes. Immunoreactive ACO protein increased by 2-fold in the diabetic group. The mRNA levels for PPAR-α, ACO and PAL increased 2.9-, 2.8- and 1.6-fold, respectively, in the diabetic group. These results suggest that the increased supply of fatty acids to liver in diabetic state stimulates the expression of PPAR-α and its target genes responsible for the metabolism of fatty acids.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006930513476
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 81
    Electronic Resource
    Electronic Resource
    Expression of calcium-binding protein regucalcin mRNA in the cloned rat hepatoma cells (Hr-II-E) is stimulated through Ca2+ signaling factors: Involvement of protein kinase C (1999)
    Springer
    Molecular and cellular biochemistry 198 (1999), S. 101-107 
    Details
    ISSN: 1573-4919
    Keywords: regucalcin ; Ca2+-binding protein ; protein kinase C ; Ca2+signaling ; gene expression ; H4-II-E hepatoma cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract The expression of hepatic Ca2+-binding protein regucalcin in the cloned rat hepatoma cells (H4-II-E) was investigated. The change in regucalcin mRNA levels was analyzed by Northern blotting using rat liver regucalcin complementary DNA (0.9 kb of open reading frame). Regucalcin mRNA was expressed in H4-II-E hepatoma cells. This expression was clearly stimulated in the presence of serum (10% fetal bovine serum). Bay K 8644 (2. 5 × 10-6 M), a Ca2+ channel agonist, significantly stimulated regucalcin mRNA expression in the absence or presence of 10% serum. Dibutyryl cyclic AMP (10-3 M) did not have a stimulatory effect on the regucalcin mRNA expression. The presence of phorbol 12-myristate 13-acetate (PMA; 10-6 M) or estrogen (10-8 M) caused a significant increase in regucalcin mRNA levels in the hepatoma cells cultured in serum-free medium, while insulin (5 × 10-9 M) or dexamethasone (10-6 M) had no effect. Bay K 8644-stimulated regucalcin mRNA expression in the hepatoma cells was completely blocked in the presence of trifluoperazine (10-5 M), an antagonist of calmodulin, or staurosporine (10-7 M), an inhibitor of protein kinase C. The stimulatory effect of PMA was clearly inhibited in the presence of stauroporine. The present study demonstrates that regucalcin mRNA is expressed in the transformed H4-II-E hepatoma cells, and that the expression is stimulated through Ca2+-dependent signaling factors.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006996506238
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 82
    Electronic Resource
    Electronic Resource
    Posttranscriptional regulation of urokinase receptor gene expression in human lung carcinoma and mesothelioma cells in vitro (1999)
    Springer
    Molecular and cellular biochemistry 199 (1999), S. 189-200 
    Details
    ISSN: 1573-4919
    Keywords: lung ; cancer ; urokinase ; receptor ; gene expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract The urokinase-type plasminogen activator (uPA) interacts with its receptor (uPAR) to promote proteolysis as well as cell proliferation and migration. These functions contribute to the pathogenesis of neoplastic growth and invasiveness. Expression of uPAR in tumor extracts also inversely correlates with prognosis in many forms of cancer. In this study, we sought to determine if differences in uPAR expression were distinguishable between cultured human lung carcinoma and malignant mesothelioma subtypes. We also sought to determine if, as in malignant mesothelioma cells, uPAR expression is regulated at the posttranscriptional level in cultured malignant lung carcinoma cells. Using 125I-uPA binding and ligand blotting techniques, uPAR was expressed by phenotypically diverse lung carcinoma cell lines, including the H460, H157 and H1395 non-small cell lines and the H146 small cell lung carcinoma line. Increased uPAR expression was also detected in spindle-shaped (M33K) and epithelioid (M9K and MS-1) malignant mesothelioma cells. Selected mediators, including TGF-β, TNF-α, LPS and PMA, uniformly enhanced uPAR expression in each of the tumor cell lines. Steady state uPAR mRNA expression was determined by RNase protection assay and correlated directly with the changes in cell surface uPAR expression. By gel mobility shift and UV-cross linking assays, a uPAR mRNA binding protein (uPAR mRNABp) implicated in the posttranscriptional control of message stability, was identified in each of the cell lines. Expression of uPAR and its message in cultured lung carcinoma and malignant mesothelioma cells is similarly influenced by effectors present in the tumor microenvironment. Regulation of the uPAR message occurs at the posttranscriptional level in cultured small and non-small cell lung carcinoma cells as well as spindle-shaped and fibrous malignant mesothelioma cell lines. Posttranscriptional regulation of uPAR in all these cells involves the interaction of the uPAR mRNABp with uPAR mRNA, which promotes uPAR mRNA destabilization.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006914800447
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 83
    Electronic Resource
    Electronic Resource
    Transcriptional modulation of the human complement factor I gene in Hep G2 cells by protein kinase C activation (1999)
    Springer
    Molecular and cellular biochemistry 201 (1999), S. 111-123 
    Details
    ISSN: 1573-4919
    Keywords: complement factor I ; TPA ; protein kinase C ; gene expression ; Hep G2 cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract This study examined the role of the protein kinase C (PKC) signalling pathway in the regulation of expression of human complement factor I (CFI) gene. The production of CFI by Hep G2 cells was enhanced in a dose- and time-dependent fashion by 12-O-tetradecanoyl-1,2-phorbol 13-acetate (TPA), a potent PKC activator. 4α-phorbol didecanoate, an inactive phorbol ester, had no effect on CFI synthesis. The TPA-dependent increase in CFI secretion was correlated with an increase in CFI mRNA levels. Forskolin, a cAMP-inducing agent, augmented the TPA response. W7, an inhibitor of protein kinase A and genistein, an inhibitor of protein tyrosine kinase(s) both did not prevent the increase in CFI expression mediated by TPA. However, calphostin C, a specific inhibitor of PKC, abolished the TPA-induced increase in CFI mRNA levels. Down regulation of intracellular PKC levels by prior exposure of Hep G2 cells to a high concentration of TPA also blocked the increase in CFI mRNA levels induced by TPA suggesting that the TPA effects were mediated via activation of PKC. mRNA decay studies indicated that the half-life of CFI mRNA in TPA-induced cells was not significantly different from control. Nuclear run-on transcriptional assays on the other hand demonstrated that whereas the CFI gene is transcribed under basal conditions in Hep G2 cells, TPA induced a 3-4 fold increase in the transcription rate of CFI gene in 24 h. The transcription rate of GAPDH gene did not change, indicating that the effects were not general on gene transcription. Transient transfections of Hep G2 cells with chloramphenicol acetyltransferase reporter gene (CAT) constructs containing a series of sequential 5′ deletions of the CFI promoter and CAT assays showed that the sequence between -136 and -130, containing an AP-1 consensus sequence (TGAGTCA) was required for the TPA response. This observation was substantiated by the finding that mutation of this AP-1 site to TttaTCA or TtAtcCA abolished the TPA responsiveness. The enhancement of the activity of transfected chimeric CAT constructs by TPA was abrogated by calphostin C and by pyrrolidine dithiocarbamate (an inhibitor of NF-κB and AP-1 transactivation). These results indicate that TPA regulation of CFI gene requires PKC signalling and is mediated by via a TPA response element (TRE) in the CFI promoter region located at -136/-130 and involves the transactivation of AP-1 and NF-κB transcription factors. We suggest that PKC may be one of the intracellular pathways that control CFI gene expression and that cellular processes (involving growth factors, hormones, cytokines etc.) that activate PKC may upregulate the expression of the CFI gene.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1007064602321
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 84
    Electronic Resource
    Electronic Resource
    Expression of calcium-binding protein regucalcin and microsomal Ca2+-ATPase regulation in rat brain: Attenuation with increasing age (1999)
    Springer
    Molecular and cellular biochemistry 200 (1999), S. 43-49 
    Details
    ISSN: 1573-4919
    Keywords: regucalcin ; calcium-binding protein ; gene expression ; Ca2+-ATPase ; brain microsomes ; aging
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract The expression of calcium-binding protein regucalcin and its effect on the microsomal Ca2+-ATPase activity in rat brain tissues was investigated. The expression of regucalcin mRNA was demonstrated by reverse transcription-polymerase chain reaction (RT-PCR) analysis in brain tissues using rat regucalcin-specific primers. Regucalcin concentration in the brain tissues was about 5 × 10-9 M as measured using enzyme-linked immunoadsorbent assay (ELISA), and this level was lowered with increasing age (50 weeks old). The presence of regucalcin (10-9 to 10-7 M) in the enzyme reaction mixture caused a significant decrease in Ca2+-ATPase activity in the brain microsomes of young rats (5 weeks old). Meanwhile, the enzyme activity was not significantly altered by the addition of calmodulin (1 or 50 μg/ml), calbindin (1 or 10 μg/ml), and S-100 A protein (5 or 25 μg/ml), which are other Ca2+-binding proteins in rat brain. The effect of regucalcin to inhibit microsomal Ca2+-ATPase activity was weakened in the brain of rats with increasing age (50 weeks old). The present study demonstrates that regucalcin is expressed in the brain, and that it can uniquely inhibit Ca2+-ATPase activity in the brain microsomes of rats. The findings suggest that regucalcin plays a role in the regulation of microsomal Ca2+-ATPase activity in rat brain tissues.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006928402184
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 85
    Electronic Resource
    Electronic Resource
    Identification of stretch-responsive genes in pulmonary artery smooth muscle cells by a two arbitrary primer-based mRNA differential display approach (1999)
    Springer
    Molecular and cellular biochemistry 197 (1999), S. 87-96 
    Details
    ISSN: 1573-4919
    Keywords: mechanical stretch ; smooth muscle cells ; differential display ; gene expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract Physical forces induce profound changes in cell phenotype, shape and behavior. These changes can occur in vascular structures as a result of pressure overload and their effects can be seen in atherosclerotic vessels in which smooth muscle cells have undergone hyperplastic and hypertrophic changes. At the molecular level, mechanical stimuli are converted into chemical ones and lead to modulation of gene expression and/or the activation of a new repertoire of genes whose encoded proteins help the cells to adapt to their microenvironment. In this study, we have used a two primer-based mRNA differential display technique to identify candidate mechano-responsive genes in pulmonary artery smooth muscle cells. As compared to the original method described by Liang and Pardee, this technique uses two arbitrary primers instead of an anchored oligo(dt) plus an arbitrary primer in the polymerase chain reaction. The chief advantages of these modifications are an increase in the efficiency of the amplification and in the identification of differentially expressed clones. Using this approach, we compared the pattern of expressed genes in cells cultured under static conditions with those in cells that were mechanically stretched (1 Hz) for 24 h in a well-defined in vitro mechanical system. Three candidate genes that showed reproducible differences were chosen for further characterization and cloning. One clone was under expressed in stretched cells and had a DNA sequence with 90% homology to the human fibronectin gene. Two other clones were highly expressed in stretched cells and had a 92% and a 83% sequence homology with human platelet-activating factor (PAF) receptor and rat insulin-like growth factor-I (IGF-I) genes respectively. Northern blot analysis confirmed low levels of fibronectin mRNA transcripts in stretched cells. In contrast, accumulation of PAF receptor mRNA occurred 30 min after mechanical stretch was initiated whereas IGF-I mRNA levels peaked at 8 h. Both mRNA levels were sustained for up to 24 h of mechanical stretching. These results demonstrate the usefulness of the two primer-based mRNA differential display that enabled us to identify and characterize alterations at the level of gene expression among matrix proteins, G-protein coupled receptors and growth factors, each of whose response to mechanical strain is different. A more complete understanding of these responses will provide further insight into the pathologic processes associated with hypertension and atherosclerosis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006966530553
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 86
    Electronic Resource
    Electronic Resource
    Characterisation of genes isolated from a potato swellingstolon cDNA library (1999)
    Springer
    Potato research 42 (1999), S. 31-42 
    Details
    ISSN: 1871-4528
    Keywords: Solanum tuberosum L. ; tuberisation ; extensin ; acyl carrier protein thioesterase ; high mobility group protein ; gene expression ; plant development
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary In screening to isolate a full-length copy of a previously isolated cDNA clone, a further three cDNAs were also isolated from a library prepared from sub-apical swelling-stolon tissue of potato (Solanum tuberosum L.). Sequence analysis showed these clones to be similar to extensin-like protein genes, acyl carrier protein thioesterase genes and high mobility group protein genes, respectively. A further cDNA, isolated by subtractive hybridisation, was similar to a tomato cDNA previously isolated on the basis of its down-regulation following nematode infection. While all the newly isolated genes were expressed in swelling stolons, for most, maximal expression was seen to be in stem tissue. Possible roles for these genes in the development of potato plants are discussed, as is the significance of gene expression in stems and stolons to the process of tuberisation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02358389
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 87
    Electronic Resource
    Electronic Resource
    Introduction of a Proximal Stat5 Site in the Murine α-Lactalbumin Promoter Induces Prolactin Dependency In Vitro and Improves Expression Frequency In Vivo (1999)
    Springer
    Transgenic research 8 (1999), S. 23-31 
    Details
    ISSN: 1573-9368
    Keywords: transgenic mice ; prolactin ; mammary gland ; gene expression ; Stat5 ; β-globin insulator
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In order to establish a possible correlation between in vitro prolactin induction and the transcriptional activity of mammary gene promoters in transgenic mice, a functional Stat5-binding site was created by means of site-directed mutagenesis at position −70 on a 560 bp murine α-lactalbumin promotor linked to a CAT reporter gene. Surprisingly, the wild-type promoter was constitutively active in vitro and could not be induced by prolactin. Introducing the proximal Stat5 site abolished this constitutive activity and resulted in prolactin dependence in both CHO-K1- and HC11-transfected cells. In transgenic mice, both the frequency of lines expressing the transgene and the prevalence of mid to late pregnancy expression were increased.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008851802022
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 88
    Electronic Resource
    Electronic Resource
    Bildgebende diagnostik bei traumatischen verletzungen der halswirbelsäule (1999)
    Springer
    European journal of trauma 25 (1999), S. 115-118 
    Details
    ISSN: 1615-3146
    Keywords: MRT ; CT ; Halswirbelsäulenverletzungen ; MRI ; CT ; Cervical spine trauma
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Abstract Traumatic injuries of the cervical spine are of significant medical and socioeconomic importance. The potential of different imaging modalities have to be known to select the optimal imaging tool. The differentiation between functional handicaps without detectable lesions and traumatic lesions is sometimes difficult. The diagnostic work-up of traumatic cervical spine lesions is based on conventional X-ray in 2 planes. The value of the different imaging modalities for the diagnostic work-up of traumatic cervical spine lesions will be discussed.
    Notes: Zusammenfassung Traumatische Verletzungen der Halswirbelsäule sind von erheblicher medizinischer und sozioökonomischer Bedeutung. Um die heute zur Verfügung stehenden bildgebenden Verfahren optimal einsetzen zu können, ist die Kenntnis der Wertigkeit der einzelnen Verfahren von entscheidender Bedeutung. Eine besondere Schwierigkeit der Diagnose liegt in der Abgrenzung möglicher struktureller Läsionen von rein funktionellen Beeinträchtigungen. Basierend auf konventionellen Röntgenaufnahmen in zwei Ebenen, wird cin Stufenschema zur Diagnostik traumatischer Veränderungen im Halswirbelsäulenbereich vorgestellt und die Wertigkeit der einzelnen bildgebenden Verfahren diskutiert.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00578719
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 89
    Electronic Resource
    Electronic Resource
    Einsatz der computertomographie in der diagnostik des stumpfen thoraxtraumas (1999)
    Springer
    European journal of trauma 25 (1999), S. 108-114 
    Details
    ISSN: 1615-3146
    Keywords: Polytrauma ; CT ; Thoraxtrauma ; Kontusionsblutung ; Pneumothorax ; Gefäßverletzung ; Trauma ; CT ; Thorax trauma ; Contusion ; Pneumothorax ; Vascular injury
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Abstract The employment and benefit of conventional chest X-ray examination in traumatic patients have been reported on by several publications. Up to now only a few studies have been published which compare the diagnostic impact of conventional chest X-ray and CT in patients with thoracic trauma. In a retrospective study in 70 patients with multiple trauma sensitivity and specificity of chest X-ray and CT of the thorax are evaluated. Evaluation was performed by 2 independent and experienced radiologists. The readers were blinded to the results and each examination was evaluated separately. The results showed a higher sensitivity of the thoracic CT examination than conventional chest X-ray. The CT of the thorax in patients with multiple trauma is an important adjunct to conventional chest X-ray and is more sensitive in the depiction of lung parenchyma as well as vascular injuries.
    Notes: Zusammenfassung Der Einsatz der konventionellen Röntgenthoraxuntersuchung bei polytraumatisierten Patienten ist in der Literatur bereits vielfach beschrieben worden. Es sind bisher jedoch nur wenige Studien zum Vergleich der diagnostischen Aussagekraft von konventionellen Thoraxaufnahmen und Thorax-CT bei Thoraxtraumen publiziert worden. In einer retrospektiven Auswertung konventioneller Röntgenaufnahmen des Thorax und der Thorax-CT bei 70 polytraumatisierten Patienten wurden die Sensitivität und Spezifität hinsichtlich pathologischer Thoraxbefunde ermittelt. Die Auswertung erfolgte durch zwei erfahrene Radiologen, wobei beide bildgebenden Verfahren getrennt voneinander und in Unkenntnis des Vorbefundes bewertet wurden. Die Auswertung des Kollektivs ergab eine höhere Sensitivität der CT-Untersuchung gegenüber der konventionellen Röntgenthoraxaufnahme bei polytraumatisierten Patienten; sie stellt somit eine wichtige Ergänzung zur konventionellen Röntgenthoraxaufnahme dar und ist sensitiver in der Erfassung von thorakalen Verletzungen, insbesondere im Bereich des Lungenparenchyms und des Mediastinums mit Gefäßen.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00578718
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 90
    Electronic Resource
    Electronic Resource
    Quantitative analysis of transcription and translation in gene amplified Chinese hamster ovary cells on the basis of a kinetic model (1999)
    Springer
    Cytotechnology 29 (1999), S. 93-102 
    Details
    ISSN: 1573-0778
    Keywords: CHO cells ; gene expression ; kinetic model ; protein secretion ; transcription ; translation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The elevation of expression levels for secreted glycoproteins by gene amplification in mammalian cells shows a saturation behavior at high levels of gene amplification. At high expression levels a drop in the secretion efficiency for the recombinant protein occurs (Schröder and Friedl, 1997), coinciding with the appearance of misfolded protein in the cell. In this communication we investigated whether additional limitations exist at the levels of transcription and translation. Four Chinese hamster ovary (CHO) cell lines expressing different amounts of human antithrombin III (ATIII) were used as a model system. A tenfold increase in the ATIII cDNA copy number from the lowest to the highest producing cell line coincided with a 38-fold increase in ATIII mRNA levels, and an 80-fold increase in the amount of intracellular ATIII levels. The data was analyzed using a simple kinetic model. The following conclusions were derived: I. The transcriptional activity for the recombinant protein is not saturated. II. Translation itself is not saturated either, but may be downregulated as secretion efficiency drops. III. Two explanations for the previously reported drop in secretion efficiency for the recombinant protein with increasing expression level are possible: A. Protein degradation is an alternative fate for translated ATIII and the fraction of ATIII degraded after translation increases as expression level is increased. B. Translation is downregulated as the secretory apparatus becomes exhausted to maintain cell viability.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008077603328
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 91
    Electronic Resource
    Electronic Resource
    Embryonic stem cell differentiation models: cardiogenesis, myogenesis, neurogenesis, epithelial and vascular smooth muscle cell differentiation in vitro (1999)
    Springer
    Cytotechnology 30 (1999), S. 211-226 
    Details
    ISSN: 1573-0778
    Keywords: cardiogenesis ; cell differentiation ; gene expression ; mouse embryonic stem cells ; myogenesis ; neurogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Embryonic stem cells, totipotent cells of the early mouse embryo, were established as permanent cell lines of undifferentiated cells. ES cells provide an important cellular system in developmental biology for the manipulation of preselected genes in mice by using the gene targeting technology. Embryonic stem cells, when cultivated as embryo-like aggregates, so-called ‘embryoid bodies’, are able to differentiate in vitro into derivatives of all three primary germ layers, the endoderm, ectoderm and mesoderm. We established differentiation protocols for the in vitro development of undifferentiated embryonic stem cells into differentiated cardiomyocytes, skeletal muscle, neuronal, epithelial and vascular smooth muscle cells. During differentiation, tissue-specific genes, proteins, ion channels, receptors and action potentials were expressed in a developmentally controlled pattern. This pattern closely recapitulates the developmental pattern during embryogenesis in the living organism. In vitro, the controlled developmental pattern was found to be influenced by differentiation and growth factor molecules or by xenobiotics. Furthermore, the differentiation system has been used for genetic analyses by ‘gain of function’ and ‘loss of function’ approaches in vitro.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008041420166
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 92
    Electronic Resource
    Electronic Resource
    Transient gene expression in mammalian cells grown in serum-free suspension culture (1999)
    Springer
    Cytotechnology 30 (1999), S. 71-83 
    Details
    ISSN: 1573-0778
    Keywords: gene expression ; HEK293(EBNA) cells ; serum-free ; transient transfection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract In order to establish a simple and scaleable transfection system we have used the cationic polymer polyethylenimine (PEI) to study transient transfection in HEK293 and 293(EBNA) cells grown in serum-free suspension culture. The transfection complexes were made directly within the cell culture by consecutively adding plasmid and PEI (direct method). Alternatively, the DNA-PEI transfection complexes were prepared in fresh medium (1/10 culture volume) and then added to the cells (indirect method). The results of this study clearly show that the ratio of PEI nitrogen to DNA phosphate is very important for high expression levels. The precise ratio is dependent on the DNA concentration. For example, using 1 μg/ml DNA by the indirect method, the ratio of optimal PEI:DNA was about 10–13:1. However, the ratio increases to 33:1 for 0.1–0.2 μg/ml DNA. By testing several different molecular weights of the polycationic polymer we could show that the highest transfection efficiency was obtained with the PEI 25 kDa. Using PEI 25 kDa the indirect method is superior to the direct addition because significantly lower DNA concentrations are needed. The expression levels of the soluble human TNF receptor p55 are even higher at low DNA compared to 1 μg/ml plasmid. The EBV-based pREP vectors gave better transient gene expression when used in 293(EBNA) cells compared to HEK293 cells in suspension culture. No differences in expression levels in the two cell lines were observed when the pC1 (CMV)-TNFR was used. In conclusion, PEI is a low-toxic transfection agent which provides high levels of transient gene expression in 293(EBNA) cells grown in serum-free suspension culture. This system allows highly reproducible, cost-effective production of milligram amounts of recombinant proteins in 2–5 l spinner culture scale within 3–5 days. Fermentor scale experiments, however, are less efficient because the PEI-mediated transient tranfection is inhibited by conditioned medium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008000327766
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 93
    Electronic Resource
    Electronic Resource
    The role of the cell cycle in determining gene expression and productivity in CHO cells (1999)
    Springer
    Cytotechnology 30 (1999), S. 49-57 
    Details
    ISSN: 1573-0778
    Keywords: cell cycle ; CHO ; flow cytometry ; gene expression ; synchronisation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Understanding the relationships between cell cycle and protein expression is critical to the optimisation of media and environmental conditions for successful commercial operation of animal cell culture processes. Using flow cytometry for the analysis of the early phases of synchronised batch cultures, the dependency of product expression on cell cycle related events has been evaluated in a recombinant CHO cell line. Although the production of recombinant protein is initially found to be cell cycle related, the maximum specific protein productivity is only achieved at a later stage of the exponential phase which also sees a maximum in the intracellular protein concentration. Subsequent work suggests that it is the batch phase/medium composition of cultures which is the major determinant of maximum specific productivity in this cell line. Furthermore the effect of the positive association between S phase and specific productivity is subordinate to the effect of batch phase/medium composition on the specific productivity of batch cultures.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008093404237
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 94
    Electronic Resource
    Electronic Resource
    Differential expression of RAB5A in human lung adenocarcinoma cells with different metastasis potential (1999)
    Springer
    Clinical & experimental metastasis 17 (1999), S. 213-219 
    Details
    ISSN: 1573-7276
    Keywords: gene expression ; immunohistochemistry ; mRNA DD ; neoplasia metastasis ; RAB5A
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract For the sake of better understanding the molecular mechanism of neoplasia, we have used the mRNA differential display technique to analyze two human lung adenocarcinoma cell lines, AGZY83-a and Anip973. Anip973 was isolated from AGZY83-a, but manifested much higher metastatic potential than the parent line. We found that a significant differential cDNA fragment in Anip973 was over-expressed, then over-expressed cDNA fragment was cloned and sequenced. It showed that the over-expressed cDNA in Anip973 was RAB5A cDNA. And the RAB5A cDNA sequence was corresponding between the two cells. To determine whether RAB5A may be differentially expressed in the two human lung adenocarcinoma cells at protein level, we further detected RAB5A protein in the two cells by using immunofluorescent method. RAB5A protein was upregulated in highly metastatic Anip973. We also detected the difference in RAB5A gene expression at RNA level in human non-small cell lung carcinoma by RT-PCR. Using immunohistochemical staining, we also examined RAB5A change at protein level in 45 cases human non-small cell lung carcinoma paraffin sections. The results proved the evidence of upregulation of RAB5A in malignant tumor, indicated over-expression of RAB5A gene was correlated with the malignant degree and metastatic potential of lung cancer(χ2 test, p 〈0.01). The RAB5A gene is a member of RAS superfamily, which can transcribe GTP-binding protein that plays an important role in signal transduction of protein trafficking at the cell surface and GDP/GTP cycle in the regulation of endocytotic membrane traffic. Thus our results indicated that over-expression of the RAB5A gene was involved in the process of transformation from AGZY83-a to the higher metastatic cell line Anip973. The result may be a powerful experimental evidence that over-expression of RAB5A gene associated with neoplasia metastasis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006617016451
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 95
    Electronic Resource
    Electronic Resource
    Effect of Portacaval Anastomosis on Glutamine Synthetase Protein and Gene Expression in Brain, Liver and Skeletal Muscle (1999)
    Springer
    Metabolic brain disease 14 (1999), S. 273-280 
    Details
    ISSN: 1573-7365
    Keywords: glutamine synthetase ; gene expression ; portacaval anastomosis ; hepatic encephalopathy ; liver ; skeletal muscle ; ammonia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The effects of chronic liver insufficiency resulting from end-to-side portacaval anastomosis (PCA) on glutamine synthetase (GS) activities, protein and gene expression were studied in brain, liver and skeletal muscle of male adult rats. Four weeks following PCA, activities of GS in cerebral cortex and cerebellum were reduced by 32% and 37% (p〈0.05) respectively whereas GS activities in muscle were increased by 52% (p〈0.05). GS activities in liver were decreased by up to 90% (p〈0.01), a finding which undoubtedly reflects the loss of GS-rich perivenous hepatocytes following portal-systemic shunting. Immunoblotting techniques revealed no change in GS protein content of brain regions or muscle but a significant loss in liver of PCA rats. GS mRNA determined by semi-quantitative RT-PCR was also significantly decreased in the livers of PCA rats compared to sham-operated controls. These findings demonstrate that PCA results in a loss of GS gene expression in the liver and that brain does not show a compensatory induction of enzyme activity, rendering it particularly sensitive to increases in ammonia in chronic liver failure. The finding of a post-translational increase of GS in muscle following portacaval shunting suggests that, in chronic liver failure, muscle becomes the major organ responsible for the removal of excess blood-borne ammonia.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1020741226752
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 96
    Electronic Resource
    Electronic Resource
    Differentiation Dependent Activation of the Myelin Genes In Purified Oligodendrocytes is Highly Resistant to Hypoglycemia (1999)
    Springer
    Metabolic brain disease 14 (1999), S. 189-195 
    Details
    ISSN: 1573-7365
    Keywords: Oligodendrocyte cultures ; glucose ; gene expression ; malic enzyme ; membrane synthesis ; myelination ; undernutrition
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract We have previously demonstrated that the developmental upregulation of myelin-specific genes in mixed glial cultures is strongly attenuated by hypoglycemia. The present study was designed to evaluate the effect of hypoglycemia on differentiation-dependent upregulation of myelin genes in purified oligodendrocyte cultures. The expression of major myelin protein genes, i.e., proteolipid protein (PLP), basic protein (BP) and myelin associated glycoprotein (MAG) were monitored by Northern blot analysis. In control cultures maintained at 6 mg/ml of glucose, the expression of all the genes upregulated rapidly, and plateaued at approximately day 4. A similar pattern of differentiation-dependent upregulation was observed for the gene encoding a lipogenic enzyme, i.e., malic enzyme (ME). In contrast to mixed glial cultures, however, this developmental gene upregulation was not significantly affected by severe hypoglycemia (approximately 0.02 mg/ml). The results indicate that the effect of glucose deprivation on oligodendrocyte genes observed in mixed glial cultures is mediated by other cells. The upregulation of the genes in differentiating oligodendrocytes was accompanied by the production of myelin-related membrane that was isolated by density gradient fractionation. In contrast to the effect on gene expression, this anabolic activity was highly dependent on glucose, as seen from a profound suppression by severe hypoglycemia.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1020614809546
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 97
    Electronic Resource
    Electronic Resource
    Genetic Variation in Spiroplasma citri (1999)
    Springer
    European journal of plant pathology 105 (1999), S. 519-533 
    Details
    ISSN: 1573-8469
    Keywords: genome ; gene expression ; mollicute ; recombination ; transposition ; virus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Spiroplasmas are members of the Class Mollicutes, wall-less prokaryotes having a high adenosine–thymidine content in their small genomes. Spiroplasma citri is a plant pathogen that inhabits phloem. Like other phytopathogenic spiroplasmas and the related phytoplasmas, it is transmitted from plant to plant by phloem-feeding leafhoppers that serve as alternate hosts for the spiroplasma as well as vectors. Genetic information in spiroplasmas is carried on a circular chromosome, on plasmids and/or in virus genomes. A picture emerging from recent research on the S. citri genome is one of frequent and often extensive variation, resulting from a number of different mechanisms. Expansion and contraction events must continually be occurring in about equal proportions so that the net genome size varies within defined boundaries. Particularly impressive are large changes in genome size that can occur in only a few generations. As with most organisms, genetic variation in S. citri results from variation in extrachromosomal DNA content, changes due to DNA replication and repair processes and changes due to recombination. The implied flux of genetic information into and out of the S. citri genome should be beneficial to the bacterium, allowing it, with its small genome size, to adapt to new environments.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008757716803
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 98
    Electronic Resource
    Electronic Resource
    Evidence of Mucin Secretion in Human Lung Adenocarcinoma Cell Lines NCIH650 and NCIH2077 and Effect of Select Secretagogues on Mucin Secretion (1999)
    Springer
    Bioscience reports 19 (1999), S. 473-483 
    Details
    ISSN: 1573-4935
    Keywords: Mucin ; lung cancer ; gene expression ; secretion ; lung adenocarcinoma
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Mucins comprise an important class of tumor-associated antigens. The objectives of the present study were (a) to establish an in vitro model system using human non-small cell lung adenocarcinoma cell lines NCIH650 and NCIH2077 (b) provide evidence that these cell lines secrete mucin in culture conditions and (c) investigate the effects of select secretagogues on mucin secretion. The cell lines were established in ACL-4 medium containing several growth factors and retinoic acid and 5% fetal calf serum. The high molecular weight glycoconjugates secreted in the culture medium were purified by ammonium sulfate precipitation and Superose 6 and Superose 12 FPLC chromatography. The purified high molecular weight glycoconjugate fraction and the carcinoma cells were shown to have mucin by dot blot, Western blot and immunohistochemical analysis, respectively, using specific antibodies to purified major mucin, HTM-1. Also, incorporation experiments with mucin precursor 3H-glucosamine demonstrated that the cells indeed synthesize high molecular weight mucins. The effects of secretagogues such as, 8-bromocyclic AMP, ionomycin, phorbol-12-myristate-13-acetate and neutrophil elastase on mucin secretion were also investigated. Only 8-bromocyclic AMP and neutrophil elastase influenced mucin secretion. These studies provided strong evidence that the lung adenocarcinoma cell lines secrete high molecular weight mucins in culture conditions and only two of the four tested secretagogues significantly increased mucin secretion. Thus, this in vitro model system may be useful in determining alterations in mucin structure, if any, in lung adenocarcinomas as well as in studying the regulation of mucin gene expression.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1020224625088
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 99
    Electronic Resource
    Electronic Resource
    Comparison of the Anticancer Effect of Free and HPMA Copolymer-Bound Adriamycin in Human Ovarian Carcinoma Cells (1999)
    Springer
    Pharmaceutical research 16 (1999), S. 986-996 
    Details
    ISSN: 1573-904X
    Keywords: adriamycin ; doxorubicin ; HPMA copolymer ; apoptosis, multidrug resistance ; gene expression ; signal transduction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Purpose. To study peculiarities and the mechanism of the anticancer effect of free and HPMA copolymer-bound ADR in sensitive and resistant human ovarian carcinoma cells. Methods. Sensitive A2780 and ADR resistant A2780/AD cells were exposed to different doses of drugs during 12, 24, 36, 48, 60, and 72 hours. Cell viability, drug accumulation, apoptosis, cellular metabolism, lipid peroxidation, DNA content and gene expression were studied. Results. HPMA copolymer-bound ADR (P(GFLG)-ADR) possessed a comparable cytotoxicity to free ADR when comparison was based on intracellular concentrations. While free ADR up-regulated genes encoding ATP driven efflux pumps (MDR1, MRP), P(GFLG)-ADR overcame existing pumps and down regulated the MRP gene. Free ADR also activated cell metabolism and expression of genes responsible for detoxification and DNA repair. P(GFLG)-ADR down-regulated HSP-70, GSr-π, BUDP, Topo-IIα, β, and TK-1 genes. Apoptosis, lipid peroxidation and DNA damage were significantly higher after exposure to P(GFLG)-ADR, as reflected by simultaneous activation of p53, c-fos in A2780 cells) or c-jun (A2780/AD) signaling pathways and inhibition of the bcl-2 gene. Differences between free ADR and P(GFLG)-ADR increased with the time of incubation and drug concentration. Conclusions. P(GFLG)-ADR overcame drug efflux pumps, more significantly induced apoptosis and lipid peroxidation, inhibited DNA repair, replication, and biosynthesis when compared to free ADR.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018959029186
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 100
    Electronic Resource
    Electronic Resource
    Magnetic resonance imaging for solitary lesions of bone: when, why, how useful? (1999)
    Springer
    Journal of orthopaedic science 4 (1999), S. 384-396 
    Details
    ISSN: 1436-2023
    Keywords: Key words: bone tumors ; radiographs ; MRI ; CT ; scintigraphy ; gadolinium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract: The radiologist and orthopedic surgeon have a wide array of imaging modalities to choose from in diagnosing and staging solitary tumors of bone. MR imaging is the examination of choice for staging solitary tumors of bone. The radiograph, however, remains the most reliable imaging modality for predicting tumor histology. Radiographic interpretation in most instances ought to be the most influential factor in determining whether further imaging is required. This article reviews the strengths and weaknesses of the various imaging modalities and attempts to present a logical, cohesive, reproducible approach to the diagnosis and evaluation of solitary tumors of bone.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s007760050121
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...