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  • 201
    Electronic Resource
    Electronic Resource
    Effect of forced motility on the noradrenaline and 5-hydroxytryptamine metabolism in different parts of the rat brain (1972)
    Springer
    Psychopharmacology 26 (1972), S. 195-200 
    Details
    ISSN: 1432-2072
    Keywords: Forced Motility ; Motor Activity ; Brain Noradrenaline and 5-Hydroxytryptamine ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The levels of 5-HT and 5-HIAA in the rat brain were measured after 1, 6, 24 and 36 h of forced motility in a treadwheel. The results showed that 5-HT metabolism had already increased in the forebrain after 1 h of exercise but later also increased in the brain stem, especially in the mesencephalon-pons-medulla regions. These changes had almost entirely disappeared after 50 min of rest. The NA levels with and without monoamine oxidase inhibitor were measured in animals after 36 h of forced motility. The results indicated an increase in NA metabolism in the brain stem.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00422106
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  • 202
    Electronic Resource
    Electronic Resource
    Conflict behavior in a heated-floor maze: Effects of oxazepam (1972)
    Springer
    Psychopharmacology 26 (1972), S. 317-320 
    Details
    ISSN: 1432-2072
    Keywords: Oxazepam ; Conflict Behavior ; Rat ; Maze
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Rats were tested in a Lashley III maze, the floor of which was heated. The animals had to learn to negotiate it in order to gain access to the unheated goal box. In the early period of training the animals were able to find the goal box rapidly and without error. Their performance then deteriorated, in a particular way which strongly evoked a conflict behavior. This deterioration in performance was progressively reduced by oxazepam administration (8 mg/kg/day).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00422708
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  • 203
    Electronic Resource
    Electronic Resource
    Improvement of performance on an attention task with chronic nicotine treatment in rats (1972)
    Springer
    Psychopharmacology 26 (1972), S. 347-360 
    Details
    ISSN: 1432-2072
    Keywords: Chronic Nicotine ; Attention Task ; Arousal ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Male, Holtzman rats were trained extensively on an attention task which required them to respond with a single lever-press to a very short, variably presented stimulus for food reinforcement, but which also required them to inhibit inappropriate responses. After performance had stabilized, two groups were treated with either nicotine base (100 Μg/kg, s.c., t.i.d.) or saline for 4 weeks. The groups were crossed-over so that the first received saline and the second nicotine for an additional 4 weeks. A 3-week saline recovery period followed. Rats were tested on the attention task daily through all periods. Analysis of the data indicated that independent of the treatment-order, rats performed more efficiently under nicotine treatment than under saline conditions. The improvement in performance was most notable in the reduction of inappropriate responding during chronic nicotine treatment. The significance of the behavioral changes in relation to proposed nicotine-induced neurophysiological changes and the two-arousal hypothesis are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00421900
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  • 204
    Electronic Resource
    Electronic Resource
    Selective increase in avoidance responding by methamphetamine in naive rats (1972)
    Springer
    Psychopharmacology 24 (1972), S. 449-455 
    Details
    ISSN: 1432-2072
    Keywords: Avoidance and Acquisition ; Methamphetamine ; Discrimination Learning ; CNS Stimulants ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Avoidance responses, extra responses (on avoidance lever) and wrong lever responses were measured in naive rats acquiring a discriminated avoidance in a 2 lever Skinner box, 30 min after methamphetamine (0.25, 0.5 and 1.0 mg/kg). The smallest dose was ineffective; 0.5 and 1.0 mg/kg significantly increased the number of avoidance responses. This increase was not associated with a significant increase in the two other responses studied. The progressive increase (from the first to second part of the acquisition session) in avoidance responses was not accompanied by a parallel increase in the other responses. The position discrimination and the discrimination of the presence or absence of the conditioned stimulus was observed to be better in the drug groups.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00423434
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  • 205
    Electronic Resource
    Electronic Resource
    Influence de la surrénalectomie, de l'hypophysectomie, de la thyroidectomie, de la castration ainsi que de la testostérone sur le comportement d'agressivité intraspécifique induit par l'apomorphine chez le rat (1972)
    Springer
    Psychopharmacology 24 (1972), S. 476-484 
    Details
    ISSN: 1432-2072
    Keywords: Agressive Behaviour ; Apomorphine ; Testosterone ; Testes ; Hypophysis ; Adrenal Glands ; Thyroid ; Castration ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The weights of testes, prostates, adrenals, thymus and the pituitary of rats with apomorphine induced aggressiveness did not differ from those without aggressiveness. Postpubertal castration, adrenalectomy or thyroidectomy did not reduce the apomorphine induced aggressive behaviour, but a considerable reduction was found after hypophysectomy. Prepubertal castration significantly reduced, but did not completely abolish the proportion of aggressive rats, especially when castration was performed on the 5th day. Prolonged treatment of male, female and castrated rats with high doses of testosterone from the time of weaning increased the proportion of aggressive animals. Less intensive treatment and treatment of adult rats were ineffective.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00423438
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  • 206
    Electronic Resource
    Electronic Resource
    Shock-elicited fighting and delta-9-tetrahydrocannabinol (1972)
    Springer
    Psychopharmacology 25 (1972), S. 218-228 
    Details
    ISSN: 1432-2072
    Keywords: Fighting ; Delta-9-Tetrahydrocannabinol ; Chlordiazepoxide ; Fixed-Interval ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The frequency with which electric shock to the feet elicited fighting in five pairs of albino rats was not altered significantly by intraperitoneal injections of delta-9-tetrahydrocannabinol (THC) in doses ranging from 0.064–6.4 mg/kg, although chlordiazepoxide reduced the frequency of such fighting in a dose-related manner. This finding held true despite manipulations of THC vehicle, injection-test interval, and the previous drug experience of the subjects. In contrast, doses of 4.0 mg/kg produced a striking reduction in lever-pressing maintained by an FI 60″ schedule of food reinforcement.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00422503
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  • 207
    Electronic Resource
    Electronic Resource
    Influence of disodium ethane-1-hydroxy-1,1-diphosphonate on vitamin D metabolism in rats (1972)
    Springer
    Pflügers Archiv 336 (1972), S. 249-262 
    Details
    ISSN: 1432-2013
    Keywords: Diphosphate ; Rat ; Vitamin D Metabolism ; Intestinal Calcium ; Absorption ; Bone Mineralization ; Renal Functions
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The metabolism and the organ distribution of double labelled vitamin D3 (1,2-3H-4-14C-cholecalciferol) has been studied in rats in which the bone mineralization and the intestinal calcium absorption have been inhibited by a large pose (10 mg P/kg s.c. for 7–14 days) of disodium ethane-1-hydroxy-1,1-diphosphonate (EHDP). The most striking difference found was a reduced accumulation of radioactive cholecalciferol and its metabolites in the kidney of EHDP-treated rats. It is unlikely that this effect was due to an unspecific alteration of the functional renal tissue since blood urea, glomerular filtration rate and renal plasm a flow remained unaltered by this dose of EHDP. The EHDP-treated rats were able to form the metabolite eluted with peak IV of the silicic acid chromatographic system, that is 25-hydroxycholecalciferol. In these vitamin D repleted rats fed a high calcium diet, the tritium deficient metabolite eluted with peak V (1,25-dihydroxycholecalciferol) was only found in the intestinal mucosa of both control and EHDP groups three days after the injection of radioactive cholecalciferol, and this in a very small amount. Therefore no definitive conclusion can be drawn as to a possible interference of EHDP treatment on the production of 1,25-dihydroxycholecalciferol. The change in the renal metabolism of vitamin D in rats treated with a rachitogenic dose of EHDP may be caused by the modifications of the calcium metabolism brought about by the diphosphonate. Its relation, if any, with the decreased calcium absorption remains to be established.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00590049
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  • 208
    Electronic Resource
    Electronic Resource
    Cardiac output, arterial and mixed-venous O2 saturation, and blood O2 dissociation curve in growing rats adapted to a simulated altitude of 3500 m (1972)
    Springer
    Pflügers Archiv 335 (1972), S. 10-18 
    Details
    ISSN: 1432-2013
    Keywords: Cardiac Output ; Blood O2 Dissociation Curve ; High Altitude ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In rats adapted to a simulated altitude of 3500 m cardiac output measured at hypoxia by the direct Fick principle was significantly lower than in the control animals (mean values 54.3 ml/min and 69.8 ml/min, resp.). The decrease of cardiac output was accompanied by an increase of arterio-venous O2 difference and a decrease of stroke volume in the adapted rats. It is suggested that the decrease of cardiac output might be related to the increase of hematocrit. The adapted rats also showed higher arterial and mixed-venous O2 content (both at hypoxia) and increased O2 capacity. Arterial O2 saturation of the animals previously exposed to simulated high altitude hypoxia was significantly higher (67.3% as against 61.2% in the controls). The standard O2 dissociation curve showed lower oxygen affinity in the blood of the adapted animals but no physiological advantage concerning the transport of O2 to the tissues was found. In another group of animals the Bohr factor was estimated and no difference was found between rat and human blood.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00586931
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  • 209
    Electronic Resource
    Electronic Resource
    Untersuchungen zur Transport-Kinetik mittelkettiger Fettsäuren am Dünndarm (1972)
    Springer
    Pflügers Archiv 335 (1972), S. 198-212 
    Details
    ISSN: 1432-2013
    Keywords: Intestinal Absorption ; Medium Chain Fatty Acids ; Kinetics ; Active Transport ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary 1. The transport of14C-octanoate by rat small intestine was determined in vitro by a modification of the method of Semenza and Mühlhaupt, and in vivo by using isolated segments or a perfusion technique in conscious rats (Haberichet al., 1968). 2. The absorption capacity of14C-octanoate in the distal ileum was significantly lower than in the proximal jejunum. 3. 14C-octanoate injected into a jejunal segment appeared rapidly in portal venous blood reaching its maximal concentration 4 min after the injection. 4. The transport of octanoate showed a saturation effect with increasing concentrations after an incubation of 4 min. However, with constant concentrations of octanoate in the medium, the transport rate was constant for 8–10 min. A similar saturation effect was also demonstrated in vivo. Values for maximal absorption rate (V max) and the half-saturation constant (K m), calculated from the Lineweaver-Burk plot, were 1.0 μMol/min and 17.7 mM, respectively. 5. Accumulation of octanoate in tissue water was demonstrated. For example, after incubation for 40 min in a medium concentration of 0.1 mM, the tissue/ medium ratio was 2.75:1. 6. Cyanide (2×10−4 M) markedly diminished the absorption rate of octanoate. 7. The absorption of octanoate was competitively inhibited by caproic acid (C6) in vitro. The inhibition constant (K i), calculated from the Dixon plot, was 47 mM. 8. Simultaneous administration of glucose and octanoate in vivo did not affect the absorption rate of the fatty acid. 9. These results suggest that the intestinal absorption of octanoate is an energy-dependant active process.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00592157
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  • 210
    Electronic Resource
    Electronic Resource
    Hypoxic pulmonary steady-state diffusing capacity for CO and alveolar-arterial O2 pressure differences in growing rats after adaptation to a simulated altitude of 3500 m (1972)
    Springer
    Pflügers Archiv 335 (1972), S. 1-9 
    Details
    ISSN: 1432-2013
    Keywords: Pulmonary Diffusing Capacity ; Alveolar-arterial O2 Pressure Difference ; High Altitude ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Steady-state pulmonary diffusing capacity for CO and alveolar-arterial O2 pressure differences were measured at hypoxia in growing rats adapted to a simulated altitude of 3500 m. The pulmonary diffusing capacity was significantly higher and the alveolar-arterial gradients were significantly lower in the adapted animals as compared with the controls exposed to hypoxia for the first time. The increased diffusing capacity could be explained entirely by the increase of blood O2 and CO capacity whereas the decrease of gradients might be explained by the increase of blood O2 capacity together with an increase of the arterio-venous O2 difference.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00586930
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  • 211
    Electronic Resource
    Electronic Resource
    Cardiac hypertrophy, capillary and muscle fiber density, muscle fiber diameter, capillary radius and diffusion distance in the myocardium of growing rats adapted to a simulated altitude of 3500 m (1972)
    Springer
    Pflügers Archiv 335 (1972), S. 19-28 
    Details
    ISSN: 1432-2013
    Keywords: Cardiac Hypertrophy ; Diffusion Distance ; High Altitude ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In growing rats adapted to a simulated altitude of 3500 m for about 4 weeks and in their controls the evolution of cardiac ventricular weight was followed. The increase of total ventricular weight found in the adapted animals can be attributed exclusively to the increase of right ventricular weight. In other adapted and control animals cardiac capillary densities, muscle fiber diameter and external capillary radius were estimated and fiber—capillary ratio and diffusion distance were calculated. There was an increase of capillary density together with a decrease of muscle fiber density, fiber-capillary ratio and diffusion distance in the right but not in the left ventricle of the adapted rats. The muscle fiber diameters, however, were larger in both heart ventricles of the rats exposed to a simulated high altitude, especially in the right ventricle. This indicates that true hypertrophy of the muscle fibers is mainly responsible for the increase of right ventricular weight. In the left ventricle, however, a hypertrophy of the muscle fibers together with a decrease of stroma components is demonstrated. The physiological importance of the shorter diffusion distance in the right ventricle of the high altitude adapted rats is discussed and it is suggested that the shorter diffusion distance may help to keep the tissue O2 partial pressure above the critical value, mainly also in extreme situations with high myocardial O2 consumption.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00586932
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  • 212
    Electronic Resource
    Electronic Resource
    Stability of firing patterns of hypothalamic neurons subjected to direct electrical or chemical stimulation (1972)
    Springer
    Experimental brain research 15 (1972), S. 29-38 
    Details
    ISSN: 1432-1106
    Keywords: Ventromedial hypothalamus ; Single units ; Spontaneous and stimulated activity ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The relationship between mean firing rate and activity pattern was studied in 177 neurons in the ventromedial hypothalamus of rats immobilized by Remyolan. Stationary epochs of spontaneous or stimulated activity containing at least 512 interspike intervals were analyzed with the LINC computer. The most frequently encountered interval histograms (IHs) were of the exponential (35%) or gamma 2 (25%) type. The IH shape remained unchanged even if the average firing rate was varied over a wide range (from 20/sec to 2/sec) by polarization through the recording microelectrode (2–5 nA, 1–10 min) or by intracarotid injection of glucose (0.1 ml, 10–25%). The coefficient of variation (CV = SD/ mean interval) was 1.03 for the spontaneous and 0.90 or 0.95 for the activity influenced by polarization or glucose, respectively. Since according to other studies sensory stimulation decreases CV by reorganization of the synaptic input, it is suggested that polarization and glucose affect predominantly the postsynaptic elements without significantly changing the presynaptic activity pattern.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00234956
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  • 213
    Electronic Resource
    Electronic Resource
    Der Einfluß von kolloidalem Ferrihexacyanoferrat (II) auf die Verteilung und Toxicität von Thallium (1972)
    Springer
    Archives of toxicology 28 (1972), S. 39-45 
    Details
    ISSN: 1432-0738
    Keywords: Ferrihexacyanoferrate (II) ; Rat ; Thallium ; Toxicity ; Metabolism ; Ferrihexacyanoferrat(II) ; Ratte ; Thllium ; Toxicität ; Stoffwechsel
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Die Verabfolgung einer 2% igen kolloidalen Lösung von Ferrihexacyanoferrat (II) (FHCF) als Trinkwasser erhöht bei der Ratte die Ausscheidungsrate von intravenös injiziertem Tl2SO4 (mit 204Tl markiert) aus Leber, Nieren und Muskulatur auf das 2,8fache. Dieser Effekt ist zeitunabhängig. Die LD 50/30 d von intravenös injiziertem Tl2SO4 wird durch FHCF-Behandlung um das 2,3fache erhöht. Für diesen Schutzeffekt ist allerdings nur das während der ersten 24 Std verabfolgte FHCF verantwortlich. Diese Befunde weisen darauf hin, daß die Schäden bei der akuten Tl-Intoxikation sehr schnell irreversibel werden.
    Notes: Abstract The administration of a 2% colloidal solution of ferrihexacyano ferrate (II) (FHCF) as drinking water increases in the rat the excretion of intravenously injected Tl2SO4 (labelled by 204Tl) from liver, kidneys and muscles by a factor of 2.8. This effect is independant of time. FHCF-treatment increases the LD 50/30 d of intravenously injected Tl2SO4 by a factor of 2.3. This protection, however, is provided by FHCF only if administered during the first 24 hours. These findings suggest that the damage resulting from acute Tl-intoxication quickly becomes irreversible.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00349626
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  • 214
    Electronic Resource
    Electronic Resource
    Teratological studies with dichlorvos vapour in rabbits and rats (1972)
    Springer
    Archives of toxicology 30 (1972), S. 29-38 
    Details
    ISSN: 1432-0738
    Keywords: Dichlorvos ; Teratology ; Inhalation ; Rat ; Rabbit ; Dichlorvos ; Teratologie ; Inhalation ; Ratte ; Kaninchen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung An Kaninchen und Ratten wurde die teratogene Wirkung von inhaliertem Dichlorvos untersucht. Im Versuch waren primipare Kaninchenweibchen der Rasse “Dutch” mit einem Anfangsgewicht von 2 bis 3 kg und primipare Rattenweibchen vom Stamm “Carworth Farm E“ mit einem Anfangsgewicht von 200 bis 300 g. Die Tiere wurden während der gesamten Trächtigkeit folgenden Konzentrationen in der Atemluft ausgesetzt: 0,25, 1,25 und 6,25 μg Dichlorvos pro Liter Luft. Ein zusätzliches Experiment am Kaninchen wurde mit 2 und 4 μg Dichlorvos pro Liter Luft durchgeführt. Die Behandlung wirkte weder beim Kaninchen noch bei der Ratte teratogen. Selbst Konzentrationen, die für die Kaninchen tödlich waren, und die eine deutliche Cholinesterasehemmung in Plasma, Erythrocyten und Gehirn der Ratten und Kaninchen hervorriefen, wurden ohne Schädigung für die Feten vertragen.
    Notes: Abstract The teratogenic potential of inhaled dichlorvos vapour for rabbits and rats was investigated on the offspring of primiparous female Dutch rabbits weighing 2 to 3 kg and primiparous female Carworth E strain rats weighing 200 to 300 g. The animals were exposed throughout pregnancy at concentrations of 0.25, 1.25 and 6.25 μg dichlorvos per litre of air. In an additional experiment pregnant rabbits were exposed to concentrations of 2 and 4 μg dichlorvos per litre of air. The results gave no indication that dichlorvos vapour is teratogenic in rabbits or rats even at exposure concentrations resulting in maternal deaths in rabbits, and causing depression of plasma, erythrocyte and brain cholinesterase activities in pregnant animals of both species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00605271
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  • 215
    Electronic Resource
    Electronic Resource
    The acute toxicity of drugs acting at cholinoceptive sites and twenty-four hour rhythms in brain acetylcholine (1972)
    Springer
    Archives of toxicology 29 (1972), S. 39-49 
    Details
    ISSN: 1432-0738
    Keywords: Mouse ; Rat ; Brain ; Light-Dark-Programming ; Circadian ; Toxicity Rhythms ; Acetylcholine ; Pilocarpine ; Oxotremorine ; Carbachol ; Physostigmine ; Neostigmine ; Atropine ; Scopolamine ; Atropine Methyl Nitrate ; Maus ; Ratten ; Gehirn ; Beleuchtungsprogramm ; Circadianer Toxicitätsrhythmus ; Acetylcholin ; Piloearpin ; Oxotremorin ; Carbachol ; Physostigmin ; Neostigmin ; Atropin ; Scopolamin ; Atropin-methylnitrat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Nach Adaptation an ein Licht-Dunkelprogramm (8.00 bis 20.00 Licht, 20.00 bis 8.00 Dunkelheit) wurde der Acetylcholingehalt (ACh) im Mittelhirn und N. caudatus von Ratten sowie im Gesamthirn von Mäusen bestimmt. Die höchsten ACh-Konzentrationen wurden bei Ratten um 24.00 gefunden; die höchsten ACh-Werte bei der Maus waren nicht signifikant von den Konzentrationen um 24.00 und 12.00 verschieden. Signifikante ACh-Minima traten im Mittelhirn der Ratte um 12.00, im N. caudatus sowie im Mäusegehirn um 18.00 Uhr auf. Während der Dunkelperiode wurde die größte Toxicität gegenüber i.v. bzw. i.p. injiziertem ACh bei Mäusen gefunden. Ähnliche zeitliche Unterschiede ergaben sich für Neostigmin, Pilocarpin und Oxytremorin. Die Toxicität von Carbachol zeigte Maxima bei 12.00 und 24.00. Für Physostigmin, einen CholinesteraseHemmstoff mit einem tertiären Stickstoff, lagen sowohl die Toxicitätsmaxima als auch die Toxicitätsminima in der Dunkelperiode. Die rhythmische Schwankung der Toxicität von i.p. verabfolgtem Atropin verhielt sich umgekehrt wie die der Cholinomimetica (außer Carbachol). Auch nach i.v. Applikation von Scopolamin und N-Methylatropin war ein entsprechender rhythmischer Einfluß auf die Toxicität nachweisbar. Die Vorbehandlung mit N-Methylatropin schwächte die Toxicität zwar ab, der Toxicitätsrhythmus gegenüber ACh und Physostigmin war aber nicht verändert. Die Toxicität von Cholinomimetica zeigt ein Maximum während der höchsten ACh-Konzentrationen im Gehirn und der höchsten Stoffwechselaktivität. Bei den anticholinergen Substanzen zeigte sich ein umgekehrtes Verhältnis. Die Beziehungen zum zentralen ACh-Gehalt sind unklar.
    Notes: Abstract Acetylcholine (ACh) levels were determined in rat midbrain and caudate nucleus and mouse whole brain in mature animals adapted 3 weeks to a programmed lighting schedule (08:00 to 20:00 h light; 20:00 to 08:00 dark). Peak ACh levels in the rat occurred at 24:00 h; in the mouse the peak at 06:00 h did not differ significantly from values obtained at 24:00 and 12:00 h. Significant trough values occurred at 12:00 h in rat midbrain and at 18:00h in rat caudate nucleus and mouse brain. In mice toxicity of I.V. and I.P. administered ACh is maximal during the dark. Neostigmine, pilocarpine and oxotremorine have toxicity patterns similar to ACh. Carbachol toxicity peaked at 12:00 and 24:00 h. With physostigmine, a tertiary anticholinesterase, peak and trough occurred during the dark. The atropine (I.P.) toxicity rhythm is a mirror image of the cholinomimetic rhythm (except for carbachol). I.V. scopolamine and atropine methyl nitrate patterns resemble atropine's but are less clear-cut. Atropine methyl nitrate pretreatment reduced ACh and physostigmine toxicity but did not alter the overall pattern. Cholinomimetic toxicity and brain ACh patterns are similar, peaking when metabolic activity is maximal. An inverse relationship holds for the anticholinergic drugs. The relationship to central ACh is uncertain.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00316513
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  • 216
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    The comparative metabolism of [14C-vinyl]dichlorvos in animals and man (1972)
    Springer
    Archives of toxicology 30 (1972), S. 9-18 
    Details
    ISSN: 1432-0738
    Keywords: 2,2-Dichlorvinyl Dimethyl Phosphate ; Dichlorvos ; Vapona ; Metabolism ; Man ; Rat ; Mouse ; Hamster ; 2,2-Dichlorvinyl-dimethylphosphat ; Dichlorvos ; Vapona® ; Stoffwechsel ; Mensch ; Ratte ; Maus ; Hamster
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung An Mäusen und Hamstern wurde die Exkretion und Retention der Radioaktivität nach Fütterung mit [1-14C-Vinyl]-Dichlorvos bestimmt. Außerdem wurde beim Menschen die Exkretion und Exhalation gemessen und die erhaltenen Werte mit den tierexperimentellen Daten von Rattenversuchen verglichen. Die Ergebnisse zeigen einen schnellen Stoffwechsel von Dichlorvos bei allen vier untersuchten Species. Die Dealkylierung zu Desmethyldichlorvos spielt bei der Biotransformation eine untergeordnete Rolle. Eine gewisse Ausnahme zeigen die Mäuse, bei denen 18,5 % der zugeführten Radioaktivität als Desmethyldichlorvos aufgefunden werden konnte. Bei allen Species kam es zu einer hydrolytischen Spaltung zu Dichloracetaldehyd, der weiter zu Dichloraetbanol reduziert wird. Der weitere Weg führt über die Ausscheidung als Glucuronid mit dem Harn. Ein Teil wird dechloriert und das14C-Atom als14CO2 oder14C-Hippursäure im Harn ausgeschieden. Im menschlichen Harn konnte Dichloraethanol nach Inkubation mitβ-Glucuronidase nachgewiesen werden, wenn hohe Dichlorvoskonzentrationen (38 μg/l) eingeatmet wurden. Auf dieser Basis könnte eine Überwachungsmethode gegen die Inhalation von zu hohen Dichlorvoskonzentrationen ausgearbeitet werden.
    Notes: Abstract The routes of excretion and retention of radioactivity following the ingestion of [1-14C-vinyl]dichlorvos by mice and hamsters have been determined. The excretion of radioactivity in urine and exhaled air of man has also been measured. The data have been compared with those for rats. The results indicate that metabolism is rapid in the four species. Dealkylation to desmethyldichlorvos is a minor route of biotransformation, except in the mouse, where desmethyldichlorvos constitutes at least 18.5 % of the administered radioactivity. Hydrolytic metabolism of dichlorvos occurs in all species and leads presumably to dichloroacetaldehyde which is further metabolized by (i) reduction to dichloroethanol and excretion in the urine as the glucuronide and (ii) dechlorination followed by incorporation of the carbon atoms into endogenous biosyntheses and excretion as [14C]carbon dioxide and [14C]hippuric acid in the urine. Dichloroethanol can be detected inβ-glucuronidase-treated urine after exposure of a human subject to high air concentrations (38 μg/l) of non-radioactive dichlorvos. This may form the basis of a method of monitoring the exposure of humans to high concentrations of dichlorvos.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00605269
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    Effects of aging on dynamic viscoelastic properties of the rat's thoracic aorta (1972)
    Springer
    Pflügers Archiv 331 (1972), S. 357-364 
    Details
    ISSN: 1432-2013
    Keywords: Thoracic Aorta ; Complex Dynamic Young's Modulus ; Age ; Serum Lipids ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In 2 groups of male albino rats (wistar-strain), 6 months and 18 months of age, dynamic Young's moduli in longitudinal direction were measured, at 5 different frequencies between 1 and 5 Hz. Each group consisted of 10 animals. Measurements were made at the natural length and at 1.2 times this length. From each animal several serum lipids were determined. It was found that: 1. measured at the natural length and 1.2 times this length, no significant difference of dynamic Young's moduli between both age groups could be demonstrated 2. concerning the dynamic Young's moduli there is significant interaction between stretch level and age 3. retraction was significantly smaller in the old rats than in the young ones 4. the old animals had a significantly higher serum lipid content than the young rats 5. no correlation between serum lipids and Young's moduli could be demonstrated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00592696
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    Effects of electrochemical stimulation of the hypothalamus on serum prolactin and LH in rats (1972)
    Springer
    Pflügers Archiv 337 (1972), S. 71-79 
    Details
    ISSN: 1432-2013
    Keywords: Rat ; Hypothalamus ; Prolactin ; LH
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Plasma levels of prolactin and LH were measured by radioimmunoassay following electrochemical stimulation of the medial preoptic area (MPO) or the arcuate nucleus (Arc.N.) in pentobarbital anesthetized proestrous rats. Differences in the secretion pattern of prolactin and LH were observed when stimulated by means of acutely or chronically implanted electrodes. Acute implantation and stimulation of the MPO resulted in no change in serum prolactin levels, whereas stimulation by means of chronically implanted electrodes evoked a marked increase in serum prolactin. The general observation was that electrostimulation in the acute experiments causes a less sharp but more prolonged prolactin and LH release from pituitary than stimulation through chronically implanted electrodes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00587874
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  • 219
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    Mesure de la vitesse de renouvellement du lactate chez le rat par perfusion de 14-C-U (l) lactate (1972)
    Springer
    Pflügers Archiv 334 (1972), S. 293-302 
    Details
    ISSN: 1432-2013
    Keywords: Lactate Turnover ; l-Lactate14C (U) ; Hypoxaemia ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary 1. Lactate turnover was measured in 24 anesthetized rats using14C-U-(l) Lactate with the priming dose-infusion technique. 2. 14C Lactate in blood was determined with a specific radio-enzymatic micromethod. 3. Lactate turnover increases with blood lactate concentration in the range 0.5 to 3 mM. In a steady state system this relationship means that a new equilibrium may be achieved at a higher pool level, between lactate production and utilization. 4. Comparing available data in four mammalian species lactate turnover at normal lactate levels was found to be approximately constant when computed on a body surface unit basis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00592164
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  • 220
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    Sexual behaviour of adult rats after a single neonatal injection of reserpine (1972)
    Springer
    Psychopharmacology 23 (1972), S. 171-179 
    Details
    ISSN: 1432-2072
    Keywords: Reserpine ; Neonatal Treatment ; Sexual Behaviour ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract To investigate whether reserpine given in the critical period of sexual differentiation of the rat brain affects adult sexual behaviour, female and male rats were given a single injection of reserpine on the fourth day after birth, and their sexual behaviour was tested at maturity. In the 11 consecutive daily tests for spontaneous sexual behaviour the reserpine treated female animals showed fewer regular behavioural and vaginal oestrous cycles than the control animals. 19 % of the reserpinized and none of the control animals had prolonged oestrous behaviour without a coincident prolonged vaginal cycle. The reseptivity quotients of the reserpine treated animals were reduced. In the tests for hormone induced sexual behaviour after ovariectomy no statistically significant differences between the number of animals responding or between the receptivity quotients occurred. The tests for spontaneous sexual behaviour in males showed that the reserpinized animals needed fewer intromissions to ejaculate than the controls. The significance of these findings is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00401192
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  • 221
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    Distribution and elimination of trimethoprim in pregnant and newborn rats (1972)
    Springer
    Naunyn-Schmiedeberg's archives of pharmacology 272 (1972), S. 369-377 
    Details
    ISSN: 1432-1912
    Keywords: Trimethoprim ; Placental Transfer ; Distribution ; Elimination ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary After a single i.v. administration of 50 mg/kg of Trimethoprim (TM) to rats in late pregnancy, a diffusion equilibrium of the folic acid inhibitor in the foetal organs is reached after 30–60 min. The TM levels in maternal as well as in foetal organs can have an inhibitory effect on folic acid reductase activity. The elimination of TM is lower in pregnant than in non-pregnant animals. The TM elimination of the foeti is dependent on the elimination capacity of the mother. Three-days-old rats eliminate TM some four times slower than mature animals owing to their reduced kidney development.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00501243
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    Calcium transport in rat small intestine in vitro and in vivo (1972)
    Springer
    Naunyn-Schmiedeberg's archives of pharmacology 273 (1972), S. 352-365 
    Details
    ISSN: 1432-1912
    Keywords: Calcium Transport ; Small Intestine ; Rat ; in vitro ; in vivo
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Intestinal calcium (Ca) transport was studied in the rat jejunum by the in vitro perfusion technique of Fisher and Parsons and in the tied loop in vivo. Mucosal uptake and absorption of Ca was examined under the following conditions: rising intraluminal Ca-concentrations (0.5–128 meq/l); inhibition of energy dependent metabolism (2,4-dinitrophenol, N2, low temperature); net water flow, out of or into the intestinal lumen; addition of strontium (Sr); pretreatment with low Ca-diet and with 6-methyl-prednisolone. The concentration-dependent Ca absorption curve rose steeply at low Ca-concentrations but changed to a slowly rising straight line above 16 meq/l Ca++. In contrast, Ca uptake into the intestinal wall was directly related to Ca concentration, was linear from the beginning and paralleled the straight part of the absorption curve. Ca absorption was decreased by inhibition of energy dependent metabolism, addition of Sr and pretreatment with prednisolone. Pretreatment with low Ca diet increased Ca absorption and direction of net water flow (“solvent drag”) had no effect on it. Mucosal uptake of Ca was similar to Ca absorption except that metabolic inhibition increased Ca uptake but decreased Ca absorption. These results are compatible with the concept of a passive mucosal uptake and of an active absorption of Ca at low intraluminal Ca concentrations with additional passive component at high Ca concentrations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00499669
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    Concentration and origin of choline in the rat brain (1972)
    Springer
    Naunyn-Schmiedeberg's archives of pharmacology 274 (1972), S. 91-106 
    Details
    ISSN: 1432-1912
    Keywords: Choline ; Blood-Brain-Barrier ; Brain ; Rat ; Lecithin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In the rat brain the concentration of choline increases with a velocity of 20.5 nMol/g×min immediately after decapitation. This is not due to postmortal disintegration of the tissue but to persisting biochemical reactions in the absence of blood flow. This rise of choline must be considered if the concentration of choline in the living animal is to be established. Taking this fact into account one arrives at a value of 27.5±1.7 nMol Choline/g wet weight. From the arterio-venous difference of choline concentrations it has been postulated that there is an efflux of 7.2±1.5 nMol/g×min from the brain into the blood. After i.v. injection of labelled choline the peak of the specific radioactivity of choline in the brain is reached in less than 1 min but amounts to only one tenth of the specific activity of choline in blood-plasma. The specific activities in both compartments decline in parallel. Thus it can be concluded a) that choline penetrates easily through the blood-brain barrier, but the concentration gradient is directed from brain to blood; and b) that choline is continuously formed in the brain, which process permanently dilutes the quantity of labelled choline and prevents the specific activities in blood and brain from becoming equal. The calculations of turnover-rates in the different reactions leading to the choline containing glycerophosphatides are based on the data obtained. On the basis of these findings it is proposed that in the brain phosphorylated derivatives of ethanolamine (phosphorylethanolamine, cytidinediphosphate-ethanolamine) can be methylated to form the respective choline derivatives.
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    URL: http://dx.doi.org/10.1007/BF00501010
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    Immersion and perfusion fixed rat adrenal medulla: The problem of mixed cells, clear cells, and the mode of secretion (1972)
    Springer
    Cell & tissue research 126 (1972), S. 261-277 
    Details
    ISSN: 1432-0878
    Keywords: Adrenal medulla ; Rat ; Cell types ; Secretion ; Influence of fixation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Das Nebennierenmark der Ratte wurde nach Anwendung verschiedener Fixationsmethoden untersucht. Nach Immersionsfixation mit Glutaraldehyd oder Osmiumtetroxyd finden sich in der Nebenniere sog. Mischzellen, helle Zellen, syncytiale oder plasmodiale Zellen, die von den Untersuchern als Artefaktbildungen angesehen werden. In allen Proben nach Perfusionsfixation wurden solche Artefaktbildungen niemals beobachtet. Bei Immersionsfixation wurde eine enge Beziehung zwischen Sekretgranula und Cytoplasmamembran beobachtet, was bei Perfusionsfixation selten der Fall war. Die heutigen Theorien über den Sekretionsmechanismus bei Nebennierenmarkzellen werden aufgrund der vorgelegten Ergebnisse diskutiert. Die Arbeit zeigt die wesentlichen Vorteile der Anwendung der Perfusionsfixation für die Untersuchung der Nebenniere.
    Notes: Summary The adrenal medulla of the rat was studied utilizing various methods of fixation. In adrenal medulla specimens after immersion fixation either with glutaraldehyde or osmium tetroxide, elements such as mixed, clear, syncytial, or plasmodial cells, believed to be of artifactual origin, are observed in all of this material examined. These elements are absent in the specimens prepared by perfusion fixation. In specimens prepared by immersion fixation, secretory granules are found in close proximity to the plasma membrane; this localization is infrequent after perfusion fixation. Current theories of the mechanism of secretion of adrenal medullary hormones are discussed on the basis of our results. This investigation demonstrates the advantage and necessity of perfusion fixation in the study of the adrenal medulla.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307220
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    The fine structure of macrophages in the enamel organ, with special reference to the microtubular system (1972)
    Springer
    Cell & tissue research 126 (1972), S. 466-482 
    Details
    ISSN: 1432-0878
    Keywords: Macrophages ; Microtubules ; Enamel organ ; Rat ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In the enamel organ of rat incisors macrophages are present in the zone of matrix formation, the transitional zone, the enamel maturation and pigmentation zone. The macrophages accumulate adjacent to redifferentiating amelocytes in the transitional zone. The macrophages phagocytize fragments of disintegrating amelocytes. In addition to the well known complement of organelles the macrophages present an elaborated microtubular system, scattered, thick filaments, a cortical feltwork of thin filaments, and spherical nuclear bodies. The microtubules emanate from “attached” and free pericentriolar satellites and radiate aster-like towards the cell surface or into pseudopods or curve along the nuclear surface for long distances, often related to nuclear constrictions. It is suggested that the microtubular system plays a prominent role in directional movement of the macrophages. The cortical filaments, if contractile, may create the cytoplasmic flow necessary for the cell motility.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00306907
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    An electron microscopic study of the normal synaptic relationships and early degenerative changes in the rat olfactory tubercle (1972)
    Springer
    Cell & tissue research 127 (1972), S. 462-482 
    Details
    ISSN: 1432-0878
    Keywords: Olfactory tubercle ; Rat ; Degeneration ; Synapse
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The olfactory tubercle of the rat was studied by electron microscopy both in the normal and after ipsilateral olfactory bulb ablation at survival times of from 14 hours to seven days. Particular emphasis was placed on synaptic structures and their changes following the lesion. Normal synapses are similar to those described in previous studies and presynaptic profiles are of at least three types. Types-A and -B contain round vesicles and form asymmetrical contacts and type-C profiles contain flattened vesicles and form symmetrical contacts. There appear to be two major types of degenerative changes. The electron-lucent type predominates at early survival times and is seen first at 14 hours. These profiles show an early reduction in numbers of vesicles with mitochondrial swelling followed by shrinkage of the profile. These profiles become increasingly electron-dense at later survival times. The second major type of degenerating profile is initially electron-dense. The earliest changes in these profiles are an increased axoplasmic density and increased microtubular density and clumping without apparent loss of vesicles. These profiles also become progressively more electron-dense at longer survival times. The observations are discussed in relation to previous reports.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00306866
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    Intercellular bridges and division patterns of rat spermatogonia (1972)
    Springer
    Cell & tissue research 127 (1972), S. 201-208 
    Details
    ISSN: 1432-0878
    Keywords: Spermatogenesis ; Rat ; Mitosis ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The pattern of intercellular cytoplasmic bridges between rat spermatogonia and between spermatocytes is illustrated from electron microscopy of serial sections. Clones, or syncytia, containing as many as 22 connected spermatogonia and as many as 74 connected spermatocytes were observed. The absence of closed rings of cells agrees with the observation that intercellular bridges are the result of incomplete cell division, rather than cell fusion. The bridges thus are a record of spermatogonial divisions within a clone. In early spermatogonial generations there is a predominantly linear arrangement. The groups of spermatocytes have more side branches. From the presence of synaptonemal complexes it is concluded that the connected spermatocytes of a given clone are in about the same developmental stage. The pattern of intercellular bridges indicates, however, that not all nuclei in a clone undergo mitosis in the same cycle. The connected cells of a clone are therefore not all of the same generation. From unconnected bridges it is assumed that new clones originate from single cells or groups of spermatogonia which separate from an existing clone.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00306802
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    Contractility and structure of adult rat seminiferous tubules in organ culture (1972)
    Springer
    Cell & tissue research 130 (1972), S. 171-179 
    Details
    ISSN: 1432-0878
    Keywords: Tubuli seminiferi ; Rat ; Structure ; Contractility ; Organ culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Isolated pieces of seminiferous tubules of adult rats were grown in organ culture for up to 8 weeks in Petri dishes on the surface of nutrient agar. The medium consisted of newborn calf serum, Eagle's minimum essential medium, glutamate and antibiotics. This method allowed observation of the contractions of the seminiferous tubules in the culture. Contractility, light and electron microscopic structure and histochemically demonstrable activities of alkaline phosphatase and ATPase of the tubule walls were studied at 1-week intervals. The contractility and alkaline phosphatase activity were maintained in the tubule wall for 3 weeks, and the activity of ATPase was maintained for 5 weeks. The thin filaments of the myoid cells, which are responsible for the contractility, were seen with the electron microscope in tubules cultured for 5 weeks. The organ culture method described in the present paper seems to be valuable for studies concerning the functioning of the myoid cells of the seminiferous tubules and the possibility that this is regulated by hormones.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00306955
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    Ultrastructural evidence for exocytosis in the median eminence of the rat (1972)
    Springer
    Cell & tissue research 131 (1972), S. 99-107 
    Details
    ISSN: 1432-0878
    Keywords: Median eminence ; Rat ; Exocytosis ; Micro-pinocytosis ; Releasing factors
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Exocytosis has been demonstrated by electron microscopy in the external zone of the median eminence of the rat. Exocytotic profiles have been observed in nerve fibres characterized by the presence of granular vesicles with median diameters of 90–103 nm and agranular vesicles of about 50 nm. In addition to the small agranular vesicles, coated vesicles of the same size have been found in many nerve fibres, suggesting that at least part of the agranular vesicles in the median eminence originate by micro-pinocytosis. The nature of the fibres showing exocytosis is discussed. Attention is drawn to the possibility of identifying types of fibres in the median eminence by the occurrence of exocytosis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307203
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    Form und Verteilung subependymaler Basalmembranlabyrinthe am Ventrikelsystem der Ratte (1972)
    Springer
    Cell & tissue research 132 (1972), S. 553-562 
    Details
    ISSN: 1432-0878
    Keywords: Basement membrane ; Brain ventricles ; Rat ; Subependymal labyrinths ; Light- and electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Mit der Perjodsäure-Bisulfit-Aldehydthionin-Färbung (Specht) lassen sich am ganzen Ventrikelsystem des Rattengehirns sub- und interependymale Strukturen lichtmikroskopisch nachweisen, die elektronenmikroskopisch als Basalmembranlabyrinthe (BML) identifiziert werden können. Verschiedene Formen dieser BML lassen sich unterscheiden. Sie stehen in Verbindung mit subependymalen Kapillaren. Die Verteilung der BML wird in Ventrikelkarten dokumentiert.
    Notes: Summary Sub- und interependymal structures, being demonstrable by a period-acid-bisulfit-aldehydthionine-method (Specht), occur in the whole ventricular system by light microscopy. They can be identified electron microscopically as basement membrane labyrinths (BML). Different shapes of these BML can be distinguished. They are in communication with the basement membrane of subependymal capillaries. The distribution of BML is documented in “ventricular maps”.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00306641
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    Ultrastructural studies on the hypothalamic neurosecretory neurons of the rat (1972)
    Springer
    Cell & tissue research 134 (1972), S. 205-225 
    Details
    ISSN: 1432-0878
    Keywords: Diabetes insipidus ; Rat ; Hypothalamo-neurohypophysial system ; Synthesis of hormones ; Light- and Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The general ultrastructural features of the hypothalamo-neurohypophysial system in rats with hereditary hypothalamic diabetes insipidus (DI-rats, Brattleboro strain) are described. There is no decisively distinguishing difference between the neurons of the supraoptic and paraventricular nuclei. The neurons of both nuclei show signs of active protein synthesis. The perikarya of the neurons are markedly hypertrophic, the nuclei are large and the nucleoli prominent. In the cytoplasm there are numerous ribosomes, abundant rough-surfaced endoplasmic reticulum and extensive Golgi complexes. However, very few neurosecretory granules are to be seen. The axons of the hypothalamo-neurohypophysial tract are likewise enlarged and the paucity of neurosecretory granules is a striking feature also in the area of the tract. The majority of nerve endings in the posterior pituitary of DI-rats are devoid of neurosecretory granules. Microvesicles are abundant in the nerve endings and there are findings which suggest that microvesicles are involved either in endoor exocytosis. The signs of active protein synthesis and the concomitant paucity of neurosecretory granules are interpreted to imply transportation of the secretory proteins in an extragranular phase. The possible mode of release of the secretory proteins from the nerve endings and the role of microvesicles therein are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307154
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    On the fine structure of the perivascular cells in the neural lobe of rats (1972)
    Springer
    Cell & tissue research 132 (1972), S. 25-34 
    Details
    ISSN: 1432-0878
    Keywords: Neurohypophysis ; Rat ; Perivascular cells ; Neurosecretion ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The purpose of this study is to investigate the ultrastructural features of perivascular cells as found in the neural lobe of the hypophysis. In particular, an inquiry was made into the nature of the relationship of such cells to neurosecretory fibers and endings. The latter, in fact, are often invaginated within the perivascular cells and enveloped by their processes; furthermore, they often reveal a certain number of empty granules as well as characteristics of degenerative nature. In the course of this study the localization of the perivascular cells has been investigated as well as that of their processes within the extensive interlobular network typical of the hypophysial neural lobe of rodents. Based on the data gathered, the hypothesis is put forward that the perivascular cells play an important role in the turnover of neurosecretory endings, both under physiological and experimental conditions, contributing thereby to the release of post-hypophysial hormones.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00310294
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    Elementary granules, small vesicles and exocytosis in the rat neurohypophysis after acute haemorrhage (1972)
    Springer
    Cell & tissue research 125 (1972), S. 277-288 
    Details
    ISSN: 1432-0878
    Keywords: Neurohypophysis ; Rat ; Exocytosis ; Freeze-etching ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Neural lobes of rats subjected to severe acute haemorrhage under sodium pentobarbitone anaesthesia were examined electron microscopically and the ultrastructure compared with that in anaesthetised and unanaesthetised controls. Changes in the localisation and numerical distribution of elementary granules and small vesicles in the neurohypophysial nerve endings of bled rats were consistent with the occurrence of exocytosis. The occurrence of “exocytotic profiles” was observed more frequently in freeze-etched tissue samples as compared with the material fixed for conventional electron microscopy. The ratio of small vesicles: elementary granules was shown to be significantly increased (P〈0.005) in the nerve endings of neural lobes from bled rats. Equally, the numbers of exocytotic profiles related to 1000 μm2 of neurohypophysial tissue area were significantly greater (P〈0.005) in bled rats.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00306626
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    Extracellular perivascular connective tissue space in the medial terminal nucleus of the accessory optic system in rats (1972)
    Springer
    Cell & tissue research 125 (1972), S. 289-294 
    Details
    ISSN: 1432-0878
    Keywords: Accessory optic system ; Rat ; Blood vessels ; Extracellular space ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Two types of capillaries were found in the medial terminal nucleus of the rat accessory optic system. Type one capillaries are surrounded by glial processes and lack a perivascular space, whereas the type two capillaries and arterioles show a distinct extracellular perivascular space often filled with collagen fibrils. An internal as well as an external basal lamina lines these spaces. No fenestration of the endothelium was observed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00306627
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    Further studies on synaptic junctions (1972)
    Springer
    Cell & tissue research 125 (1972), S. 415-431 
    Details
    ISSN: 1432-0878
    Keywords: Synapses ; Rat ; Cerebral cortex ; Glutaraldehyde/E-PTA ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Synaptic junctions in intact rat cerebral cortex have been examined following glutaraldehyde fixation and phosphotungstic acid (PTA) staining. In the presynaptic ending the network has a hexagonal arrangement, while the dense projections are regularly placed along the presynaptic membrane. Cleft densities occupy the intracleft region. The postsynaptic thickening extends uninterrupted along the length of the junction. Qualitatively, the majority of junctions fall into the ‘discontinuous-continuous’ category, in which the internal coat of the presynaptic membrane together with its associated dense projections is discontinuous along the length of the junction, whereas the postsynaptic thickening is continuous. By contrast, a small number of junctions are ‘continuous-continuous’. In an attempt to analyze the junctions quantitatively, nine indices were measured. Histograms of the size distributions of seven of these appear to be bimodal, and from this it is concluded that two junction populations may be distinguishable on quantitative grounds. It is also shown that the distance separating dense projections at the presynaptic membrane is of the order of 10–15 nm. This surprisingly low value has consequences for current ideas on the relationship between synaptic vesicles and dense projections, and these are discussed at length.
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    Die Feinstruktur der interstitiellen Grundsubstanz im inneren Nierenmark der Ratte bei verschiedenen Fixierungsarten (1972)
    Springer
    Cell & tissue research 125 (1972), S. 404-414 
    Details
    ISSN: 1432-0878
    Keywords: Renal medulla ; Rat ; Interstitial ground substance ; Fixation ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Das Interstitium des inneren Nierenmarkes von Ratten wurde nach Anwendung verschiedener Fixierungsarten und Versuchsanordnungen elektronenmikroskopisch untersucht. Neben Bindegewebszellen und kollagenen Fibrillen enthält der interstitielle Raum bandförmige, basalmembranartige Strukturen, in deren weiträumigen Maschen zwei weitere Komponenten, nämlich ein feingranuläres und feinfilamentäres Material sowie kontrastreiche, globuläre Partikel liegen. Diese beiden Formelemente stellen sich bei den verschiedenen Fixierungsarten und Versuchsanordnungen unterschiedlich dar. Bei der feingranulären und feinfilamentären Komponente handelt es sich wahrscheinlich um interstitielles Blutplasma. Diese Deutung wird gestützt durch die morphologische Ähnlichkeit mit dem Blutplasma der Blutgefäße, die unterschiedliche Darstellbarkeit nach Glutardialdehyd-und Formalinfixierung sowie durch die Befunde nach Gefäßperfusion und Nierenvenenstauung. Das Verhalten der globulären Partikel nach OsO4-Einwirkung steht in Einklang mit der schon früher gegebenen Interpretation als präzipitierte saure Mukopolysaccharide. Ihr elektronenoptisches Bild wird im Vergleich zur bekannten makromolekularen Struktur der Mukopolysaccharid-Protein-Moleküle diskutiert.
    Notes: Summary The interstitial ground substance in the inner medulla of the rat kidney is examined electron microscopically after fixation with various fixation agents and under different experimental conditions. The papillary interstitium contains besides interstitial cells and few collagen fibrils basement membrane like material and two other components, fine granular-fibrillar elements and globular particles with heavy contrast. The appearance of the two latter interstitial components varies according to the different fixation agents used and to the experimental conditions. The fine granular and fine fibrillar material surrounds the globular particles numerously after glutardialdehyde-fixation but scarcely after formaldehyde-fixation. It increases after renal vein occlusion, but is diminished or absent, if the kidney is rinsed bloodfree. These results allow to conclude that this material represents interstitial bloodplasma. The behaviour of the globular particles after OsO4-fixation agrees well with the earlier interpretation according to which they represent precipitated acid mucopolysaccharides. Their electron microscopic appearance is discussed in comparison with the known macromolecular structure of the acid mucopolysaccharides.
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    URL: http://dx.doi.org/10.1007/BF00306634
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    Further studies on synaptic junctions (1972)
    Springer
    Cell & tissue research 125 (1972), S. 432-447 
    Details
    ISSN: 1432-0878
    Keywords: Synaptosomes ; Synapses ; Rat ; Cerebral cortex ; Glutaraldehyde/E-PTA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Working with glutaraldehyde fixed, PTA stained rat cerebral cortex, the authors compared the ultrastructural features of synaptosomes with those of intact synaptic junctions. In general there is close correspondence between the two, although the cleft densities and postsynaptic focal densities of synaptosomes show a greater degree of focalization than their counterparts in synaptic junctions. The dense projections have similar profiles in both preparations, but are more difficult to distinguish clearly in synaptosomes on account of the closer packing of the presynaptic network around their apices. The limiting membrane of the presynaptic terminal is usually visible in synaptosomes, but not in synaptic junctions. Comparing the preparations quantitatively reinforces the qualitative findings, and points to their overall similarity. However a number of the indices in synaptosomes are significantly smaller than the corresponding ones in synaptic junctions, and this points to the operation of a shrinkage factor during fractionation procedures. This is confined to the pre- and post-synaptic components and does not affect the intervening contact region. Histograms of the size distributions of the indices are similar to those obtained for intact synaptic junctions, the majority displaying two peaks. It is concluded that synaptosomes accurately reproduce the major ultrastructural features of synaptic junctions.
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    URL: http://dx.doi.org/10.1007/BF00306652
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    On the fine structure of alteration of the adrenal cortex in hypophysectomized rats (1972)
    Springer
    Cell & tissue research 125 (1972), S. 480-496 
    Details
    ISSN: 1432-0878
    Keywords: Adrenal cortex ; Rat ; Normal ; Hypophysectomy ; Structural alteration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The adrenal cortex of normal and hypophysectomized rats was observed by electron microscopy 5–90 days after the operation and, chiefly, the degeneration mechanism of the cortical tissue was discussed. In normal rats mitochondrial inner structures are generally tubular in shape in glomerulosa cells, vesicular (and tubulo-vesicular) in fasciculate cells and tubulo-vesicular (and vesicular) in reticularis cells. Some mitochondria in a few glomerulosa cells show long straight tubular crests in normal as well as hypophysectomized rats. Usually lipid droplets in normal glomerulosa, fasciculate and reticularis cells are surrounded by elements of smooth endoplasmic reticulum, which are very closely associated with mitochondria. About 10–25 days after hypophysectomy, most mitochondria in fasciculate and reticularis cells show tubular crests, and numerous electron lucent lipid droplets are accumulated in these cells. These droplets occupy most parts of the cytoplasm and are sometimes in contact with one another or with mitochondria. The elements of smooth endoplasmic reticulum and Golgi apparatus are markedly reduced in number and size. About 10–90 days after the hypophysectomy, the degenerative cortical cells containing irregularly shaped nuclei, heterogeneously dense bodies in various sizes, and a large number of electron lucent lipid droplets are seen. The author speculates that most fasciculate and reticularis cells degenerate because of a disorder in the lipid metabolism, since ACTH release, which is necessary for synthesis of corticosteroid hormones from cholesterol, is absent in hypophysectomy. The fatty degeneration of the fasciculate and reticularis cells is considered to be a main cause for attenuation of the cortical tissue. Sometimes atrophied cortical cells without lipid droplets are also found 60–90 days after hypophysectomy.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00306655
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    The manchette in Stage 14 rat spermatids: A possible structural relationship with the redundant nuclear envelope (1972)
    Springer
    Cell & tissue research 124 (1972), S. 1-11 
    Details
    ISSN: 1432-0878
    Keywords: Spermiogenesis ; Rat ; Spermatids ; Manchette ; Microtubules
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The manchette or caudal tube has been examined in Stage 14 rat spermatids. The microtubules of the caudal tube have been found to be partially sheathed by smooth endoplasmic reticulum which appears to be continuous with the outer nuclear membrane of the redundant nuclear envelope. The microtubules in caudal regions of the manchette have been noted to be interconnected by links of unusual size and morphology. It is suggested that the caudal tube consists at this stage of development of two structures, membrane and microtubules and that the links between the microtubules appear to play a role in the structural order noted in the position of the tubules of the manchette. The possible significance of these links in relation to motility is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00981937
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    Ultrastructural study of neurovascular contacts in the median eminence of the rat (1972)
    Springer
    Cell & tissue research 124 (1972), S. 311-319 
    Details
    ISSN: 1432-0878
    Keywords: Median eminence ; Rat ; Neurovascular contacts ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In the median eminence of the rat axons of the supraoptic-paraventricular-hypophyseal tract with elementary neurosecretory granules (150–200 mμ) traverse the internal zone. Terminals containing dense core vesicles 60–120 mμ in diameter end on the portal capillaries of the median eminence. A unique organisation of the primary portal capillaries is shown. Endothelial cells have many fenestrae. The pericapillary space has numerous extensions all of which represent a special zone around the endothelial tube. The fine structural organisation and function of the neurovascular contacts in the median eminence are discussed.
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    URL: http://dx.doi.org/10.1007/BF00355033
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    Pituitaries of rats neonatally treated with oestrogen (1972)
    Springer
    Cell & tissue research 126 (1972), S. 41-52 
    Details
    ISSN: 1432-0878
    Keywords: Pituitary ; Rat ; Oestradiol influence ; STH-cells ; Leucine incorporation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Pituitaries of male and female rats neonatally treated with oestradiol dipropionate were examined with light and electron microscopy, disc electrophoresis and in vitro C14-leucine incorporation. Single dose of oestradiol administered shortly after birth to male rats has an prolonged stimulative effect on LTH synthesis and release. This is expressed in the cell hypertrophy, proliferation of ER, increase of corresponding band density and higher rate of 14C-leucine incorporation. In females this effect was less pronounced. STH cell in the treated male pituitaries were degranulated, corresponding hormone band density significantly lowered and incorporation of 14C-leucine was reduced. This could be considered as an inhibitory effect of oestradiol on STH synthesis and release. Such effect was more expressed two than four months after treatment and is more pronounced in male than in female rats.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00306779
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    Autoradiographische Untersuchungen zum Wachstum der Nebennierenrinde der Ratte (1972)
    Springer
    Cell & tissue research 126 (1972), S. 421-430 
    Details
    ISSN: 1432-0878
    Keywords: Adrenal cortex ; Rat ; Growth ; Sex-dimorphism ; Autoradiography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Anhand des Verlaufs des 3H-Index und der Mitoserate in Anhängigkeit vom Lebensalter wurde die Proliferationsaktivität in den Zonen der Nebennierenrinde untersucht. 84 SPF-Ratten erhielten 2 μCi/g 3H-Thymidin i.p.; der Untersuchungszeitraum erstreckte sich vom 18. Trächtigkeitstag bis zur 12. Lebenswoche. Alle Zonen zeigten in der Rangfolge Glomerulosa — Fasciculata — Reticularis eine Abnahme DNS-synthetisierender Zellkerne. Geschlechtsspezifische Unterschiede im Ausmaß der Proliferationsaktivität konnten zu keiner Zeit nachgewiesen werden. Aus der Dissoziation der Kurven des 3H-Index und der Mitoserate bei vergleichbaren DNS-Syntheseraten wird auf eine Änderung der G2-Phase des Generationszyklus der NNR-Zellen in der teilexponentiellen Wachstumsphase geschlossen.
    Notes: Summary In 84 SPF-rats the poliferative activity of the adrenal cortical cells was studied from the 18th day of pregnancy up to 12 weeks post partum. Rats were given 2 μC/g tritiated thymidine and killed 1 hour thereafter. It was shown that there was no sex-related difference in the degree of proliferation that could explain the sexual dimorphism in adrenal weights. In all cortical zones a decrease in the number of labeled cells was seen during the obvservation period. The highest percentage of labeled cells was found in the glomerulosa. There exists no parallelism of the mitotic and labeling index, which gives evidence for a change in length of the G2-phase of the cell generation cycle.
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    URL: http://dx.doi.org/10.1007/BF00306854
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    Ultrastructural features of the lateral preoptic area, median eminence and arcuate nucleus of the rat (1972)
    Springer
    Cell & tissue research 127 (1972), S. 483-491 
    Details
    ISSN: 1432-0878
    Keywords: Lateral preoptic area ; Hypothalamus ; Rat ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The arcuate nucleus, median eminence, and the lateral preoptic area from the brains of aldehyde-perfused male and female rats were examined by electron microscopy. In the lateral preoptic area, three neuronal types are described: a small light neuron, a larger light one, and a dark neuron resembling the larger light one in size and nuclear shape. Many myelinated axons are interposed among single neurons or neuronal pairs. The relationship of structures to each other is discussed. Several observations not previously reported are illustrated from tissue of the arcuate nucleus and median eminence.
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    URL: http://dx.doi.org/10.1007/BF00306867
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    Distribution of monoamine oxidase in the hippocampal region of the rat (1972)
    Springer
    Cell & tissue research 124 (1972), S. 354-366 
    Details
    ISSN: 1432-0878
    Keywords: Hippocampal region ; Rat ; Monoamine oxidase ; Chemoarchitectonics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary 1. The distribution of monoamine oxidase (MAO) in the hippocampal region of the rat has been studied in cryostat sections with the tryptamine-tetrazolium method of Glenner et al. 2. The staining pattern was rather diffuse as compared with the distribution of MAO in the guinea pig and of other enzymes in the rat hippocampal region. 3. However, there were differences in enzyme activity between different areas and layers which could be correlated to some degree with results obtained with other methods, e.g., silver impregnation, catecholamines, and acetylcholinesterase.
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    URL: http://dx.doi.org/10.1007/BF00355036
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    Monoamines in rat thyroid parafollicular cells and the effect of vitamin D2-induced degranulation (1972)
    Springer
    Cell & tissue research 128 (1972), S. 406-425 
    Details
    ISSN: 1432-0878
    Keywords: Parafollicular Cells ; Rat ; Normalcalcemia ; Vitamin D2 ; Electronmicroscopy ; Histochemical fluorescence method
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Thyroid parafollicular cells of normocalcemic and vitamin D2-treated rats were investigated by electron microscopy and with the histochemical fluorescence technique of Hillarp and Falck. Administration of high doses of vitamin D2 caused hypercalcemia and an extensive degranulation of the parafollicular cells. The formation and storage of monoamines in granulated and degranulated parafollicular cells was investigated by fluorescence microscopy after injection of monoamine precursors (DOPA, 5-HTP), alone or in combination with Ro 4-4602, nialamide or reserpine. No fluorescence was observed in parafollicular cells of untreated rats. l-DOPA and l-5-HTP (but not the corresponding D-amino acids) were taken up by a process closely linked to the decarboxylation of the amino acids to the corresponding amines (dopamine and 5-hydroxytryptamine). Treatment with vitamin D2 did not seem to affect the formation of amines in the parafollicular cells or the formation and storage of amines in other cell systems investigated. The amine itself (dopamine) was not taken up by the parafollicular cells. In normocalcemic rats, the amine formed was retained in the cytoplasm of the parafollicular cells by a partially reserpine-resistant mechanism. The storage of amines is concluded to occur in association with the calcitonin-containing granules. In parafollicular cells of vitamin D2-treated rats, a certain amount of amine was bound in the cytoplasm in the absence of typical granules. As a considerable amount of calcitonin is known to remain in the thyroid of vitamin D2-treated rats, the present observations may indicate an association between the amine and the polypeptide hormone calcitonin, whether the latter is confined to typical granules or not.
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    URL: http://dx.doi.org/10.1007/BF00306979
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    Electron microscope study of the myofibril partial disintegration and recovery in the mitotically dividing cardiac muscle cells (1972)
    Springer
    Cell & tissue research 129 (1972), S. 471-499 
    Details
    ISSN: 1432-0878
    Keywords: Cardiac muscle cells ; Rat ; Mitotic division ; Myofibril changes ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The ultrastructure of the myocyte at all phases of mitosis as well as of early postmitotic cells has been studied in the myocardia of 14- and 18-day rat embryos and 5- and 7-day old rats. The myofibrils remain unchanged up to the late prophase. In prometaphase the majority of Z-disks in embryo myocyte myofibrils and considerable part of these disks in myofibrils of suckling rats are drastically disintegrated. This is followed by a progressive isolation and scattering of the myofilament bundles and of the whole sarcomeres during the subsequent phases of mitosis. Thick myofilaments seem to be unchanged but thin ones become frequently poorly outlined (mainly in embryos). The sarcoplasmic reticulum, including its typically differentiated subsarcolemmal cisternae, exhibits relatively few changes during mitosis. In the early postmitotic period there is a gradual restoration of contrast-rich Z-bands, interconnecting the previously isolated sarcomeres. Patterns of this process have much in common with early stages of myofibrillogenesis (appearance of subsarcolemmal “Z-bodies”, formation of skeins of thin filaments etc.). The cleavage furrow formation is either absent or considerably retarded up to the postmitotic period. Behaviour of some other organelles during myocyte mitosis has been described. Possible mechanisms and significance of the observed phenomena are discussed.
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    URL: http://dx.doi.org/10.1007/BF00316744
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    Zur Ultrastruktur der Gefäßfortsätze von Ependymund Gliazellen im Infundibulum der Ratte (1972)
    Springer
    Cell & tissue research 130 (1972), S. 58-69 
    Details
    ISSN: 1432-0878
    Keywords: Infundibulum ; Rat ; Ependymal and glial cells ; Inclusions ; Neuroglial synapses
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Zahlreiche Ependymzellen des Recessus infundibularis und Gliazellen der Zona externa besitzen Fortsätze, die bis an den perivaskulären Raum der Kapillarschlingen des Portalplexus heranreichen. Die Ultrastruktur dieser Gefäßfortsätze wird beschrieben. Sie können verschiedenartige Einschlüsse enthalten: 1. große, runde osmiophile Einschlüsse (0,5–2 μ im Durchmesser), die als „lipid inclusions“ bezeichnet werden; 2. unregelmäßig geformte, granuläre Einschlüsse mit gleichmäßiger Elektronendichte (500 bis 2000 Å im Durchmesser); 3. rundliche Granula mit einem hellen Zentrum und ringartig um dieses Zentrum gelagerten elektronendichten Körnchen (1200–1600 Å im Durchmesser). Der letztgenannte Granulatyp scheint ein charakteristisches Merkmal der Gefäßfortsätze in der Zona externa der Wistar-Ratte zu sein. Meist häufen sich die Körnchen gemeinsam mit den anderen beschriebenen Einschlüssen in den kolbenförmigen Endigungen der Gefäßfortsätze. Exocytosevorgänge an der dem perivaskulären Raum zugewandten Oberflächenmembran der Fortsätze weisen auf eine Abgabe von Substanzen an die Blutbahn hin. Neurogliöse Synapsen finden sich im Infundibulum vorwiegend zwischen Nervenfasern des Tractus tuberohypophyseus und Gefäßfortsätzen der Ependym- und Gliazellen. Der synaptische Spalt enthält häufig fädige oder körnige Strukturen. Solche Partikel finden sich zusammen mit tubulären oder vesikulären Membranprofilen auch im synapsennahen Bereich des Glia- oder Ependymfortsatzes. Die funktionelle Bedeutung dieser Synapsen insbesondere für die Abgabe neuronaler Substanzen wird diskutiert.
    Notes: Summary Many ependymal cells of the infundibular recess and many glial cells of the external layer send their processes to the perivascular space of capillary loops of the portal plexus. The ultrastructure of these vascular processes is described. They may contain various inclusions: (1) large, osmiophilic globules (0,5–2 μ in diameter), mostly termed “lipid inclusions”; (2) irregularly formed, granular inclusions with an evenly distributed content (500–2000 Å in diameter); (3) circular granules comprising an electron-lucent centre surrounded by an annular wall of electron-dense particles (1200–1600 Å in diameter). This type of granule seems to be characteristic for the vascular processes in the external layer of the Wistar rat. Frequently accumulations of these granules are found together with other inclusions in the widened end-feet of the vascular processes. The occurrence of exocytosis at the perivascular surface membrane of the vascular processes indicates a release of substances into the blood. Neuro-glial synapses in the infundibulum are mainly found between nerve fibres of the tuberohypophyseal tract and the vascular end-feet of ependymal and glial cells. The synaptic cleft often contains filamentous or granular material. Together with tubular and vesicular membrane profiles such particles are also found in the postsynaptic area of the ependymal and glial processes. The functional significance of these contacts is discussed particularly with respect to the release of neuronal substances.
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    URL: http://dx.doi.org/10.1007/BF00306994
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    Isolation and characterization of neurosecretory granules of the rat posterior pituitary gland (1972)
    Springer
    Cell & tissue research 129 (1972), S. 92-113 
    Details
    ISSN: 1432-0878
    Keywords: Neurosecretion ; Neurohypophysis ; Neurosecretory granule ; Rat ; Membranes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Neurosecretory granules (NSG) of rat posterior pituitary glands were prepared by differential centrifugation techniques mainly according to the procedure as described by Barer, Heller and Lederis (1963). As revealed by electron microscopy, the recovery of neurophysin and the contents of enzymes, purified NSG were obtained in a pellet at 30 000 g/60 min (0.44 M sucrose). Eighteen h after injection of (35S) cysteine into the supraoptic nucleus 60% of the recovered radioactivity in the neural lobe was found in the NSG, whereas 20% was found in the final supernatant (100 000 g/120 min). Sixteen days after injection the NSG and the final supernatant fraction contained fairly equal amount of (35S) cysteine (approximately 40%). It is suggested that after a period of intragranular maturation neurophysin is extruded into an extragranular pool of neurosecretory material. With the use of conventional polyacrylamide-gel electrophoresis it was shown that the predominating proportion of radioactivity in the NSG after a hypothalamic injection of (35S) cysteine was located within the neurophysin fraction A and in fraction B. Fraction B is suggested to be partly bound to the NSG membranes. When the NSG soluble and NSG insoluble proteins, obtained after lysis of NSG, were separated on polyacrylamide gels in the presence of sodium dodecylsulphate, the highly radioactive soluble protein was shown to consist of two components with average molecular weights of 12 300 and 14 600. Most of the proteins in the lysate were found in the NSG membranes, though less radioactive. A component with a mol.wt. of 37 000 was enriched in the membrane fraction. At longer times after isotope injection the high mol.wt. proteins, particularly those of the NSG membranes, contained increased amounts of radioactivity.
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    URL: http://dx.doi.org/10.1007/BF00307113
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    Electron microscopy of filaments in the basal part of rat kidney tubule cells, and their in situ interaction with heavy meromyosin (1972)
    Springer
    Cell & tissue research 132 (1972), S. 497-521 
    Details
    ISSN: 1432-0878
    Keywords: Kidney ; Rat ; Actin- and myosin-like filaments ; Tubule and interstitial cells ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary By electron microscopy, the prominent bundles of filaments occurring in the basal part of proximal and distal tubule cells and in interstitial cells of rat kidney cortex were studied in cells fixed by vascular perfusion, in glycerol-extracted cells and in glycerol-extracted cells treated with heavy meromyosin (HMM). The studies of perfusion-fixed tissue showed that the proximal tubule cells contained in their most basal part filamentous bundles oriented transversely around the tubule. The bundles consisted of trightly packed thin filaments (50–80 Å in diameter). Similar but less prominent bundles were found in distal tubule cells and in interstitial cells. The dimension of these filaments was similar to that of actin filaments and their insertion in the basal cell membrane of the tubule epithelial cells resembled the insertion of actin filaments in the cell membrane of smooth muscle cells. The studies on glycerol-extracted cells revealed that some tubule cells contained two types of filaments (60–80 Å and 130–170 Å in diameter) located side by side in the basal filamentous bundles. The dimension of the thick filaments corresponds well to the values for myosin filaments in glycerinated smooth and skeletal muscle. The studies on HMM-reacted renal tissue revealed that the thin filaments (60–80 Å) described in tubule and interstitial cells are probably actin filaments, as they formed characteristic arrowhead complexes morphologically indistinguishable from the complexes of HMM with actin filaments in smooth and striated muscle cells. Our results provide strong evidence that a two-filament contractile system, based on interaction of actin and myosin filaments, exists in renal tubule and interstitial cells. As a hypothesis it is proposed that it is changes in tonus of the basal filamentous system in the proximal tubule cells which stabilize the intratubular pressure, possibly via angiotensin.
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    URL: http://dx.doi.org/10.1007/BF00306638
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    The manchette in Stage 14 rat spermatids: A possible structural relationship with the redundant nuclear envelope (1972)
    Springer
    Cell & tissue research 124 (1972), S. 1-11 
    Details
    ISSN: 1432-0878
    Keywords: Spermiogenesis ; Rat ; Spermatids ; Manchette ; Microtubules
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The manchette or caudal tube has been examined in Stage 14 rat spermatids. The microtubules of the caudal tube have been found to be partially sheathed by smooth endoplasmic reticulum which appears to be continuous with the outer nuclear membrane of the redundant nuclear envelope. The microtubules in caudal regions of the manchette have been noted to be interconnected by links of unusual size and morphology. It is suggested that the caudal tube consists at this stage of development of two structures, membrane and microtubules and that the links between the microtubules appear to play a role in the structural order noted in the position of the tubules of the manchette. The possible significance of these links in relation to motility is discussed.
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    URL: http://dx.doi.org/10.1007/BF00335450
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    Ribosomes in myelinated axons of dorsal root ganglia (1972)
    Springer
    Cell & tissue research 124 (1972), S. 217-229 
    Details
    ISSN: 1432-0878
    Keywords: Sensory axons ; Dorsal root ganglia ; Rat ; Ribosomes ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In the dorsal root ganglia of the rat, ribosomes were found not only in the initial segment, but they were also observed in the axoplasm of intraganglionar myelinated fibres and in the sensory portion of spinal nerves. Axons of seven-days-old rats contained more ribosomes than those of adult animals. The amount of particles decreased gradually from the initial segment trough intraganglionar internodes to the axons of spinal nerves. No ribosomes were found in axons of dorsal roots. In intraganglionar fibres, ribosomal particles were usually observed near the nodes of Ranvier, in the vicinity of Schmidt-Lantermann clefts and in axons near the Schwann cell nuclei. They were arranged in tetrads, pentads or in larger polysomes, and they were often observed adjacent to a group of mitochondria. The particles had invariably a stable size, their average diameters measuring 234 ± 2 × 197 ± 3 Å, which is practically equal to the diameters of 232 ± 2 × 203 ± 3 Å of ribosomes in the Schwann cell cytoplasm. These values fall within the range of diameters of ribosomes isolated from various cells of eukaryotic organisms as given in the literature. Since no other granular component of the cytoplasm has similarly stable dimensions, the measurements are considered to prove that the axonal particles described here are ribosomes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00335680
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  • 252
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    Cellules riches en glycogène dans l'épendyme du ventricule latéral du cerveau de rat (1972)
    Springer
    Cell & tissue research 123 (1972), S. 356-368 
    Details
    ISSN: 1432-0878
    Keywords: Ependyma ; Brain ; Rat ; Glycogen-rich cells ; Electronmicroscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The ependyma of the lateral ventricle of the rat brain was investigated at different ages from 20 days to adulthood. A particular cell type occurs in the external wall of the ventricle, where the proliferative subependymal layer is present. These cells found at all ages studied, are characterized by a high content of glycogen, and a structure different of typical ependymal cells. A large number of nerve endings is situated in close vicinity of these cells, either free in the ventricle lumen, or sometimes ensheathed in the cells. No synapse was found between these endings an the glycogen-rich cells. These glycogen-rich cells undergo several modifications with age: their glycogen content is reduced in the adult, and they acquire a few cilia and gliofilaments. It is suggested that they represent a transitory differentiation of the ependyma, functionally linked with the proliferative subependymal layer.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00335637
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  • 253
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    Peripheral sympathetic innervation and serotonin cells in the habenular region of the rat brain (1972)
    Springer
    Cell & tissue research 127 (1972), S. 570-579 
    Details
    ISSN: 1432-0878
    Keywords: Sympathetic nerve fibres ; Rat ; 5-HT-cells ; Habenular region (Lamina intercalaris ; Medial habenular nucleus)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The pineal gland of the rat is located near the brain surface and is via a slender stalk connected to lamina intercalaris which constitutes a cell formation between the habenular and posterior commissures, continuing to the subcommissural organ. The stalk and lamina intercalaris, like the pineal proper, exhibited a yellow, formaldehyde-induced fluorescence which showed the histochemical and pharmacological properties of 5-HT. All these structures were richly supplied with catecholamine-fluorescent nerves which could be further followed rostrally from lamina intercalaris, mixing with the non-fluorescent commissural fibres and stria terminalis, into the medial habenular nucleus in which they extensively supplied both blood vessels and non-fluorescent nerve cells. Cytospectrofluorometric and chemical analysis suggested that the fluorescent nerves stored noradrenaline. This was supported by the finding that they disappeared after bilateral cervical sympathectomy (as did the fluorescent nerves in the pineal complex). In the medial habenular nucleus also catecholamine-containing and 5-HT-containing nerves of central origin were present. The occurrence of a rich, peripheral sympathetic innervation in the medial habenular nucleus of the brain offers possibilities for a previously not observed sympathetic influence on this nucleus. Also the arrangement, and the apparent continuity of the sympathetic innervation in the pineal gland, the lamina intercalaris, and the medial habenular nucleus, suggests some functional interconnection or coordination between these structures.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00306872
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  • 254
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    Release in vitro of neurohypophysial proteins from neural lobe tissue slices and from isolated neurosecretory granules of the rat (1972)
    Springer
    Cell & tissue research 129 (1972), S. 114-139 
    Details
    ISSN: 1432-0878
    Keywords: Neurosecretion ; Pituitary gland ; Rat ; Neurophysin ; Vasopressin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The release of neurophysin from neural lobe tissue slices and isolated neurosecretory granules of the rat was studied at various time intervals after injection of (35S) cysteine into the supraoptic nucleus. For hours after isotope injection the release of radioactive neurophysin from neural lobe tissue was increased by depolarizing concentration of potassium in the presence of calcium ions. Fourteen and 30 days after isotope injection the release of radioactive neurophysin was relatively decreased in a medium of high potassium concentration which might be explained by the heterogeneity of the pool of neurophysin within the neural lobe. Four h after isotope injection the “spontaneous” release of neurophysin from neural lobe tissue was higher in dehydrated rats than in controls, and the neural lobes of these animals did not respond with an increased release of radioactive neurophysin when exposed to high potassium concentration. Eighteen h after isotope injection the predominating proportion of neurophysin-bound radioactivity was found in the neurosecretory granule fraction, whereas 14 days after injection a fairly equal amount of radioactivity was found in this fraction and in the “soluble” protein fraction. This indicates that with time an increasing amount of radioactive neurophysin passes from an intragranular to an extragranular pool. The spontaneous release of radioactive neurophysin from isolated neurosecretory granules was higher, and the increase of release upon exposure to an ATP-regenerating system was higher 14 days after isotope injection than 18 h after injection. This may imply that the neurosecretory material undergoes an intragranular “maturation” process to become more easily releasable. The release of radioactive neurophysin was inhibited in the presence of AMP and EDTA, which demonstrates the dependence of the release process of an ATPase and, probably, of calcium ions. Neurosecretory granules from dehydrated rats possessed a higher spontaneous release than those of control rats 18 h after injection, which may indicate an enhanced intragranular “maturation” process of the neurosecretory material due to osmotic stimulation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307114
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  • 255
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    Zur Größe der Fettpartikel in mitochondrienreichen Skelettmuskelfasern der Ratte in Abhängigkeit von der Nahrungsaufnahme (1972)
    Springer
    Cell & tissue research 127 (1972), S. 374-381 
    Details
    ISSN: 1432-0878
    Keywords: Skeletal muscle ; Rat ; Fat droplets ; Fat resorption ; Quantitative electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Elektronenmikroskopisch nachweisbare Neutralfettpartikel kommen im M. tibialis anterior der Ratte vorwiegend in den mitochondrienreichen Fasern vom Typ C in der Nachbarschaft von Mitochondrien vor. Nach Glutaraldehyd-Osmium-Fixierung und Epon-Einbettung sind diese Partikel meist dielektronisch, seltener teilweise oder ganz adielektronisch. Auf 100 μm2 Schnittfläche durch C-Fasern findet man 3–4 Anschnitte von Fettpartikeln. Der mittlere Durchmesser der Anschnitte (d) ist 0,69 μm, der mit der graphischen Methode von Hennig bestimmte mittlere Durchmesser der Fettpartikel (d′) 0,80 μm. 2 h Narkose mit Pentobarbital beeinflussen weder Anzahl noch Durchmesser der Anschnitte. Nach 2 h Resorption von Maiskeimöl ist die Zahl der Anschnitte unverändert, der mittlere Durchmesser der Anschnitte bzw. der Partikel hat um etwa 20 % zugenommen (d=0,83 μm; d′=0,95 μm). Das entspricht einer Zunahme des morphologisch nachweisbaren Neutralfettes um 70 %. Da keine Pinocytose von Chylomikronen zu beobachten ist, wird angenommen, daß freie Fettsäuren durch das Plasmalemm in die Muskelfaser eindiffundieren und im Bereich vorhandener Fettpartikel als Triglyceride gespeichert werden.
    Notes: Summary In the anterior tibial muscle of rats triglyceride droplets predominantly appear within fibres rich in mitochondria (type C). In electron micrographs 3–4 of such droplets are present per 100 μm2 sectional area of C-fibres. The mean diameter of sections through fat droplets (d) is 0.69 μm, the mean diameter of the droplets (d′) as determined by Hennig's graphical method is 0.80 μm. Anaesthesia with pentobarbital (2 h) does affect neither number nor size of the particles. 2 h after application of corn oil into the stomach the number of fat droplets is unaltered, but the mean diameter has increased by 20 % (d=0.83 μm; d′=0.95 μm), corresponding to an increase of the volume of about 70 %. Pinocytosis of chylomicrones by muscle fibres has never been observed. Therefore it is supposed, that free fatty acids have passed the plasmalemma by diffusion and are incorporated into triglycerides and stored within pre-existing fat droplets.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00306880
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  • 256
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    An ultrastructural study of the neurosecretory cells of the medial vascular prechiasmatic gland (1972)
    Springer
    Cell & tissue research 127 (1972), S. 439-461 
    Details
    ISSN: 1432-0878
    Keywords: Prechiasmatic gland (Organon vasculosum laminae terminalis) ; Rat ; Nerve endings ; Dense-cored vesicles ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary On the basis of the present electron microscopic study of the rat median vascular prechiasmatic gland, the unmyelinated nerve fibers containing dense-cored vesicles may be classified in three categories. (1) Nerve endings containing isomorphic rounded dense-cored vesicles, 800–1600 Å in diameter with a predominance of vesicles between 1100 and 1300 Å. These vesicles are synthesized in the nerve cell perikarya which are localized principally in the lamina profunda of the outer subpial layer and also in the neuropil of the inner glial covering layer. One or more “cholinergic” axon terminals are in contact with the surface of these nerve cell perikarya. The dense-cored vesicles travel in the axonal process which branches into an extensive terminal network characterized by the presence of small varicosities. They abut on the pericapillary spaces but never against another neuron or effector cell. A few nerve processes course between the ependymal cells and terminate in the preoptic recess of the third ventricle. The dense-cored vesicles may contain one of the primary monoamines. (2) Nerve endings containing pleomorphic dense-cored vesicles, 500–1600 Å in diameter with a predominance of vesicles between 800 and 1100 Å. The latter granular vesicles are associated with small, clear-centered vesicles, 260–550 Å in diameter, which are often aggregated in the vicinity of thickenings of the plasma membrane and of cytoplasmic dense projections. The intercellular space and the cell plasma membrane, however, do not display any modification facing these structures. Synapse-like contacts are observed between these nerve endings and cells containing a great amount of microfilaments and glycogen particles in their cytoplasm. The latter cells possibly represent processes of ependymal cells. (3) Nerve endings containing a few large dense-cored vesicles in addition to a majority of synaptic vesicles.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00306865
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  • 257
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    Adrenocorticotropin production by the intermediate lobe of the rat pituitary (1972)
    Springer
    Cell & tissue research 132 (1972), S. 1-14 
    Details
    ISSN: 1432-0878
    Keywords: Pituitary ; Rat ; Pars intermedia ; Adrenocorticotropin ; Immunohistochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Adrenocorticotropin (ACTH) was localized in the intermediate lobes of normal rat pituitaries with unlabeled antibody and the soluble peroxidase-antiperoxidase complex at the electron microscopic level. All of the intermediate lobe cells (light and dark cells) stained positively for ACTH. Stain of varying intensity was found on secretory granules and vesicles. Structures which resembled rough endoplasmic reticulum stained where the secretory granules and vesicles were most numerous. No stain was on mitochondria, in nuclei, in cisternae and granules of Golgi complexes, or in rough endoplasmic reticulum where there were few secretory granules. There were distinct immunohistochemical differences between the pars intermedia and anterior lobe ACTH cells. In the ACTH cells of the anterior lobe, the Golgi complex and its granules stained strongly, but not at all in the pars intermedia cells. This suggests that ACTH may not be packaged or condensed in the Golgi region of the latter.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00310292
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  • 258
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    Sub- und interependymale Basalmembranlabyrinthe am Zentralkanal der weißen Ratte (1972)
    Springer
    Cell & tissue research 132 (1972), S. 217-229 
    Details
    ISSN: 1432-0878
    Keywords: Basement membrane labyrinths ; Spinal cord ; Central canal ; Rat ; Light- and Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Die Wand des Zentralkanals im Rückenmark der weißen Ratte enthält zahlreiche Basalmembranlabyrinthe; ihre Zahl nimmt in caudaler Richtung deutlich zu. Die Perjodsäure-Bisulfit-Aldehydthionin-Färbung (Specht) gestattet ihren lichtmikroskopischen Nachweis. Die Labyrinthe stehen in Verbindung mit den Basalmembranen der kanalnahen Kapillaren. Sie können auch elektronenmikroskopisch identifiziert werden; ihre Struktur gleicht der der Basalmembranen der Gefäße. Die funktionelle Bedeutung der Basalmembranlabyrinthe wird diskutiert.
    Notes: Summary The wall of the central canal of the spinal cord of the white rat contains numerous basement membrane labyrinths, their number increasing towards the end of the canal. The period-acid-bisulfit-aldehydethionine-method (Specht) permits their light microscopic finding. They are in connection with the basement membranes of the capillaries situated near the canal. The basement membrane labyrinths can be identified by electron microscopy; their structure is similar to that of basement membranes of the capillaries. The functional significance of these structures is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307012
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    Surface multivesicular structures associated with maturing erythrocytes in rats (1972)
    Springer
    Cell & tissue research 133 (1972), S. 181-186 
    Details
    ISSN: 1432-0878
    Keywords: Surface multivesicular structures ; Erythrocyte maturation ; Rat ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Surface multivesicular structures associated with the plasmalemma of erythrocytes were observed in the peripheral blood of rats which have a significant number of circulating reticulocytes. These surface structures appear as ovoid evaginations (0.2 to 0.7 μ in diameter) of the plasma membrane and contain numerous small vesicles ranging from 0.05 to 0.1 μ in diameter. The structures were present during the final stages of maturation of erythrocytes, after nuclei and mitochondria had been extruded and only a few polysomes and small vesicles remained. They appear quite distinct from the autophagic vacuoles which have been described in association with degeneration and extrusion of mitochondria from erythrocytes. The exact origin of the small internal vesicles of these surface multivesicular structures is unknown; however, similar vesicles have been observed in the cytoplasm of the maturing erythrocyte especially in the vicinity of the Golgi body. These structures suggest a process by which Golgi elements and other small cytoplasmic vesicles are extruded during the late stages of maturation of rat erythrocytes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307140
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  • 260
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    Zur Morphologie und Funktion der Calcitoninzellen (C-Zellen) der Ratte (1972)
    Springer
    Cell & tissue research 134 (1972), S. 13-30 
    Details
    ISSN: 1432-0878
    Keywords: Rat ; Thyroid C-cells ; Ultrastructure ; Tocopherol and Ubiquinone deficiency ; Substitution (d,1-α-Tocopherol, α-Tocopherolquinone)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung An der Schilddrüse von Wistar-Ratten wurde der Einfluß der Kost (Laborstandardkost, halbsynthetische-tocopherolhaltige und halbsynthetische-tocopherol-freie Kost) und der subkutanen Injektion von Tocopherol und Tocopherolchinon auf die Ultrastruktur der C-Zellen untersucht. Während nach Standardfütterung die Aktivität der C-Zellen der Norm entspricht, werden Syntheseleistung und Sekretabgabe dieser Zellelemente durch die halbsynthetische-tocopherolhaltige Kost gesteigert und im alimentären Tocopherol-Ubichinonmangel wieder gedämpft. Der elektronenmikroskopisch erkennbare Gehalt der C-Zellen an Sekretgranula entspricht der in vitro ermittelten Calcitoninaktivität. Die Beziehungen zwischen den Funktionskreisen von Thyreozyten und Calcitoninzellen werden diskutiert.
    Notes: Summary The influence of different diets (standard laboratory diet, semisynthetic tocopherol-containing and semisynthetic tocopherol-free diet) and the influence of subcutaneous injections of tocopherol and tocopherolquinone upon the ultrastructure of the C-cells in the thyroid gland of Wistar rats were investigated. While the activity of the C-cells is quite normal after feeding a standard diet, the synthetic and secretory activities of these cells show an increase with a semisynthetic diet containing tocopherol, but a decrease during alimentary lack of tocopherol and tocopherolquinone. The amount of secretory granules in the C-cells as seen with the electron microscope corresponds with the in vitro-activity of extracted calcitonin. The relations between the functional system of the thyrocytes and that of the C-cells are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307233
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  • 261
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    Effect of foreign innervation on the androgen-sensitive levator ani muscle of the rat (1972)
    Springer
    Cell & tissue research 135 (1972), S. 165-174 
    Details
    ISSN: 1432-0878
    Keywords: Musculus levator ani ; Rat ; Androgen sensitivity ; Foreign innervation ; Histochemical Muscle fibre pattern
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Cross-union of the tibial with the pudendal nerve innervating the androgen-sensitive levator ani (LA) muscle of male rats, results in reversal of the histochemical muscle fibre pattern concerning myofibrillar ATPase, succinate dehydrogenase and phosphorylase enzyme activities. The homogeneous muscle fibre pattern of the LA muscle is changed to a mosaic pattern of muscles normally innervated by the tibial nerve. The success of the hetero-reinnervation is shown by practically full recovery of muscle weight and of isometric twitch-contraction properties of the LA muscle. Castration of 2-months duration, i. e. lack of the male sex hormone, leads to marked atrophy but no change in histochemical muscle fibre pattern. Hetero-reinnervation of the LA muscle results in change of histochemical enzyme pattern even if the cross-union of nerves is performed after long periods of castration leading to very marked decrease of muscle fibre size. However, testosterone application alone after castration increases markedly muscle fibre size but does not lead to reversal of muscle fibre pattern. The myotropic hormonal influence on the target (LA) muscle is therefore primarily of myogenic origin and specificity of hormonal action is maintained even with a foreign nerve innervating the muscle. The experiments, thus, provide evidence for the differentiation of specific neural influences affecting muscle fibre pattern and hormonal influences in respect to the myotropic action of the sex hormone on the androgen-sensitive LA muscle.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00315124
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  • 262
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    Autoradiographische Bestimmung der S-Phasen-Dauer der Gonocyten bei der Wistarratte durch Einfach- und Doppelmarkierung (1972)
    Springer
    Cell & tissue research 125 (1972), S. 229-251 
    Details
    ISSN: 1432-0878
    Keywords: Gonocytes ; Rat ; Mitotic activity ; Autoradiography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Die Gonocyten der Ratte können in 2 hintereinander geschaltete Keimzellarten gegliedert werden, die I-Gonocyten und II-Gonocyten. Die I-Gonocyten proliferieren bei der Wistarratte zwischen 15. und 18. Fetaltag, die Tochterzellen der I-Gonocyten, die II-Gonocyten treten nach einer Zeitdauer von 7–8 Tagen zwischen 4. und 6. Lebenstag in die Mitose. Auf Grund der gewonnenen Daten erschien es sinnvoll, die mitotische Aktivität der II-Gonocyten und die Bestimmung der Dauer ihrer S-Phase an 5 Tage alten Ratten durchzuführen. Untersuchungen von 50 Zentren mitotischer Aktivität in einem in Serie geschnittenen Hoden einer 5 Tage alten Ratte ergaben, daß 148 von 190 Mitosen, d.s. 78%, in Gruppen und 122, d.s. 64% der Mitosen in „Paaren“ vorkommen. Mit der Methode der markierten Mitosen (Quastler u. Sherman, 1959) und der Methode der Doppelmarkierung (Hilscher u. Maurer, 1962) wurde die Dauer der S-Phase der II-Gonocyten bei 5 Tage alten Ratten bestimmt. Es ergab sich eine gute Übereinstimmung der nach beiden Methoden bestimmten Werte. Die S-Phasen-Dauer der II-Gonocyten dürfte danach am 5. Lebenstag bei 11,0–11,5 Std liegen.
    Notes: Summary The gonocytes of the rat are of two types: I-gonocytes and II-gonocytes. In Wistar rat I-gonocytes proliferate at the beginning of prespermatogenesis between the 15th and 18th day of gestation. Their multiplication stops between the 18th and 19th day. Starting on the 4th postnatal day, II-gonocytes, the daughter cells of I-gonocytes, begin to proliferate. The 5th postnatal day proved to be favourable for studying the mitotic activity and for determing the S-phase of II-gonocytes. In one serially sectioned testis of a 5 days old rat 25 sex cords were reconstructed. Till now 50 centres of mitotic activity of II-gonocytes with 190 mitoses were localized. Only 42 out of the 190 mitoses were isolated, 148 occur in groups. 122 out of the grouped mitoses are in “pairs”. That means that 78% of the grouped and 64% of all mitoses were to be found in pairs. By the method of labeled mitoses (Quastler and Sherman, 1959) and by the method of double labeling with C-14- and H-3-thymidine (Hilscher and Maurer, 1962) the duration of the S-phase of II-gonocytes were determined in 5 days old rats. The results of both methods show that the S-phase is 11.0 to 11.5 hours.
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    URL: http://dx.doi.org/10.1007/BF00306791
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    Metabolisches Verhalten von kolloidalem Ferrihexacyanoferrat(II) (1971)
    Springer
    Naunyn-Schmiedeberg's archives of pharmacology 269 (1971), S. 48-56 
    Details
    ISSN: 1432-1912
    Keywords: Colloidal Ferrihexacyanoferrate(II) ; Potassium Hexacyanoferrate(II) ; Metabolism ; Toxicity ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Intestinal absorption, distribution, and excretion of ferrihexacyanoferrate(II), labeled by59Fe at the Fe(III)- and Fe(II)-atoms, respectively, was studied in rats. Following i.v. injection, the compound is separated at ∼60% into Fe3+ and [Fe(CN)6]4−, the residual fraction being retained by the reticuloendothelial system. [Fe(CN)6]4− is excreted rapidly and virtually completely by the kidneys. With oral administration the disintegration amounts to ∼7%, and ∼2% of [Fe(CN)6]4− is absorbed from the gut. No evidence was obtained for decomposition of [Fe(CN)6]4−. No toxic side-effects were observed after chronic administration of ferrihexacyanoferrate(II).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01422015
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    Ultrastructure of rat uterine luminal epithelium at functional states compatible with implantation (1971)
    Springer
    Anatomy and embryology 135 (1971), S. 101-107 
    Details
    ISSN: 1432-0568
    Keywords: Rat ; Uterus ; Luminal epithelium ; Ultrastructure ; Implantation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The ultrastructure of the rat uterine luminal epithelium has been examined during normal pregnancy, pseudopregnancy, lactation and lactation-pregnancy, all being conditions when a blastocyst is known to be able to implant. In all four groups of animals the epithelium passes through three characteristic and structurally similar states, namely a pre-attachment state, an attachment state and a postattachment state. The attachment state is characterized by a close contact between apposing luminal surfaces of the epithelium. It is concluded that implantation in an undamaged uterus claims a sequence of certain changes in the uterine luminal epithelium, and that the blastocyst attachment occurs within the early part of that period when the luminal epithelium is in the attachment state.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00525195
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  • 265
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    Licht-und elektronenmikroskopische Untersuchungen zum Problem der Blut-Hirn-Schranke circumventriculärer Organe der Ratte nach Behandlung mit Myofer (1971)
    Springer
    Anatomy and embryology 134 (1971), S. 278-297 
    Details
    ISSN: 1432-0568
    Keywords: Rat ; Ferro-dextran ; Blood brain barrier ; Circumventricular organs
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Nach Vorbehandlung mit Myofer (Eisen III-Dextran) wurde die Aufnahme der Testsubstanz in den circumventriculären Organen von Rattengehirnen mit dem Licht-und Elektronenmikroskop untersucht. Die Ergebnisse zeigten, daß ein partieller Schrankeneffekt zwischen Gefäß und Gehirn auch in diesen Regionen besteht. Im allgemeinen ist der Schrankeneffekt die Folge hintereinandergeschalteter Barrieren, deren morphologisches Substrat 1. das Gefäßendothel und 2. die Gliagrenzschicht sind. Im Parenchym der verschiedenen Gehirnteile ist die Endothelzelle für diese gegebene Substanz undurchlässig. Dabei wird Myofer zwar in die Zelle aufgenommen, aber total zurückgehalten. In den circumventriculären Organen des Rattengehirns (Area postrema, Epiphyse, Subfornikalorgan, Organum vasculosum laminae terminalis, Neurohypophyse, Plexus chorioidei) ist die Endothelzelle durchlässig. Daher findet man Myoferpartikel im perivasculären Raum. Der. „Hemmeffekt” der zweiten Barriere ist abhängig 1. von der Konzentration des Myofers in dem perivasculären Raum und 2. von der Zeitdauer, während der die Gliagrenzschicht beeinflußt wird. Die perivasculären Gliafortsätze nehmen als einzige Zellelemente Myofer auf. Da der Anteil von Zellen mit Gliacharakter in den circumventriculären Organen wechselt, ist in den einzelen Regionen Myofer in unterschiedlicher Menge nachweisbar. Neuronale Elemente enthalten niemals Myofer.
    Notes: Summary After pretreatment of rats with Myofer (ferro-dextran complex) the uptake of this substance into circumventricular organs of the brain has been studied by means of light and electronmicroscopy. The results show that in these regions a partial barrier effect exists between blood and brain parenchyma. Generally the barrier effect is the result of impediments, which are connected in series. The morphological substratum of them is (1) the endothelium of the capillaries and (2) the limiting layer of the glial processes. In the parenchyma of the brain (cerebellum) the endothelial cell is sealed for this substance: the Myofer is taken up by the endothelium, but retained in the cells. In the circumventricular organs of the rat's brain (area postrema, epiphysis cerebri, subfornical organ, Organum vasculosum laminae terminalis, neurohypophysis, choroid plexus) the endothelial cells are leaky. Consequently one finds particles of Myofer inside the perivascular space. The inhibitory effect of the second barrier depends (1) on the concentration of Myofer in the perivascular space and (2) on the period, during which the limiting layer of the glial processes has been affected. The perivascular glial processes are the only cell elements which take up Myofer. Since the proportion of cells characterized as glial elements changes between the circumventricular organs, Myofer is detectable in different amounts within the various regions. Neuronal elements never contain Myofer.
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    URL: http://dx.doi.org/10.1007/BF00519916
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    Electron microscopic observations on the development of the median eminence in perinatal rats (1971)
    Springer
    Anatomy and embryology 134 (1971), S. 311-327 
    Details
    ISSN: 1432-0568
    Keywords: Development ; Median eminence ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary This paper reports details of the development of the external layer of the median eminence with special reference to the appearance of the nerve terminals, containing electron dense core and electron lucent vesicles, in the outermost layer of a palisade structure (2–3 μ thick). Observations were made on perinatal rats of the Sprague-Dawley strain (16, 18, 20 day fetuses and rats 5 hours, one 4, and 8 days after birth). The palisade structure of the outermost layer of the median eminence was first clearly detectable 5 hours after birth. Concomitantly, there was a marked increase in the number of nerve terminals in this layer. The nerve terminals containing electron dense core and electron lucent vesicles were seen in 18 day fetuses in the outermost layer of the median eminence. These nerve terminals increased in number during development, especially from the 20th day of fetal life to the 4th day after birth. The size range of the majority of the electron lucent vesicles did not change during development, but that of the core vesicles increased from 600–800 Å from the 18th to 20th day of fetal life. The initiation of neurohemal control of the adenohypophysial function by the hypothalamus is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00519918
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    Effects of60Co radiation on the cellular responses in degenerating dorsal columns in the rat spinal cord (1971)
    Springer
    Acta neuropathologica 19 (1971), S. 318-322 
    Details
    ISSN: 1432-0533
    Keywords: Co-Radiation ; Spinal Cord ; Rat ; Cell Proliferation ; p-Bromophenylacetylurea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Rats were irradiated with doses of 1 000–3 000 rads to the cervical spinal cord and subsequently given a paralytic dose of p-Bromophenylacetylurea. The nuclear populations in the degenerating dorsal columns were determined and it was found that a significant suppression of cell proliferation occurred after all three dose levels. The cell populations in the shielded parts of the tracts rostral to the irradiated zone were not affected.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00692152
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    Über die akuten Veränderungen im Parietalcortex der Ratte nach spitzem Trauma (1971)
    Springer
    Acta neuropathologica 19 (1971), S. 249-264 
    Details
    ISSN: 1432-0533
    Keywords: Brain Edema ; Stab Wound ; Neocortex ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The parietal cortex of rats was examined by light and electron microscopy 1–120 min after a standardized stab wound (250×450×1800μm, constant stab velocity). The changes in the tissue are already visible qualitatively after 1 min. After about 4 min the expansion of tissue changes stops. 4 zones may be separated. Surrounding the stab canal concentrically they are relatively sharply defined. Zone I. Stab canal, haemorrhagical or “debris zone”, primary traumatic destroyed zone. The tissue units are here completely destroyed. Zone II. “Squashed” or “indirectly but irreversibly damaged” zone. It is ca. 80μm wide and contains apart from a protein-rich extracellular fluid almost exclusively swollen cells and cell fragments. Zone III. Swelling brain or “intracellular peritraumatic edema”. It is ca. 150μm wide and contains mainly dark neurones and swollen astroglia. Zone IV. Transitional zone of variable width. Here only the perivascular and perineural processes are swollen without changed neurone structure. In all swollen astrocytes mitochondria are altered typically (denser matrix, dilated cristae). Discussed are: The short latency period, Zone IV, causes of astroglial swelling, origin of extracellular fluid as well as mechanisms which limit the spread of extracellular fluid into Zone II.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00684602
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    An anatomical study of the efferent connections of the thalamic reticular nucleus (1971)
    Springer
    Experimental brain research 12 (1971), S. 435-446 
    Details
    ISSN: 1432-1106
    Keywords: Thalamic reticular nucleus ; Ascending reticular system ; Topo graphical arrangement ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A neuro-anatomical investigation into the thalamic reticular nucleus and its connections was made in the albino rat, using the method of retrograde degeneration and the Nauta-Gygax method. The results correspond to the description given by Scheibel and Scheibel and indicate connections of the neurons of the reticular which the principal thalamic nuclei and the rostral part of the intralaminar thalamic nuclei. A topographical arrangement of the efferent connections of the reticular nucleus is demonstrated. The projection of the reticular nucleus upon the thalamus duplicates the cortico-thalamic connections.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00234497
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    Effects of chronic amphetamine intoxication on the accumulation in the rat brain of labelled catecholamines synthesized from circulating tyrosine-14C and dopa-3H (1971)
    Springer
    Naunyn-Schmiedeberg's archives of pharmacology 271 (1971), S. 211-233 
    Details
    ISSN: 1432-1912
    Keywords: Amphetamine ; Synthesis of Catecholamines ; Brain Catecholamines ; Release of Catecholamines ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Accumulation of labelled noradrenaline (NA) and dopamine (DA) and endogenous levels of NA and DA in brain and heart were measured after infusion of tyrosine-14C and dopa-3H in rats. After a single dose of 20 mg/kg i.p. of dl-amphetamine sulphate a 50–65% decrease in the NA accumulation in the brain and heart was observed. The accumulation of DA was not changed. In rats treated chronically with amphetamine sulphate, 16−32 mg/kg i.p. twice daily for 4 weeks, both the brain and heart NA and brain DA levels were depleted by 40–70% at 20–24 h after amphetamine withdrawal. The accumulation of labelled NA and DA in these animals was not different from that of saline treated controls. When amphetamine sulphate, 20 mg/kg i.p., was given to chronically amphetamine treated rats there was a 45–60% decrease in the accumulation of labelled NA; labelled DA remained unchanged. It is concluded that the decrease in NA accumulation under acute and chronic amphetamine intoxication is most likely due to a preferential release of newly synthesized NA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00997217
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    Effect of chlorpromazine on conditioned avoidance as a function of CS-US interval length (1971)
    Springer
    Psychopharmacology 22 (1971), S. 144-150 
    Details
    ISSN: 1432-2072
    Keywords: Avoidance ; Chlorpromazine ; CS-US Interval ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Three groups of four rats learned to avoid electric shock (US) by turning a wheel in response to a buzzer stimulus (CS). The CS-US intervals for each group were 5, 10, and 20 sec, respectively. After each animal had learned the avoidance procedure and had achieved a stable level of performance, the effect of several doses of chlorpromazine on percent avoidance, on latency time from CS onset to termination by a response, and on response rate was determined as a function of CS-US interval length. No consistent relationship between increasing interval length and response rate was observed. Neither lengthening the CS-US interval nor the interaction of this lengthening with chlorpromazine dose was found to exert a statistically significant effect on percent avoidance. Furthermore, although a statistically significant increase in response latency was found to be associated with increasing CS-US interval length, the increases in latency noted were not of sufficient magnitude to corroborate the hypothesis that lengthening the CS-US interval contributes importantly to increased avoidance responding in animals tested with chlorpromazine. Further, the results of this study do not support induction of a locomotor deficit as the mechanism by which chlorpromazine suppresses the avoidance response.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00403622
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    The effects of chronic administration of ethanol on startle thresholds in rats (1971)
    Springer
    Psychopharmacology 19 (1971), S. 95-104 
    Details
    ISSN: 1432-2072
    Keywords: Ethanol ; Tolerance ; Dependence ; Withdrawal Reaction ; Startle Threshold ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The thresholds for startle responses to electric shock were measured in adult male Wistar strain rats given ethanol daily in doses rising from 3 to 7 g/kg over a 30-day period, and in controls receiving equicaloric doses of sucrose. Tests made 23, 36, or 47 h after ethanol (i.e., during partial or complete ethanol withdrawal) gave threshold values significantly lower than those obtained with sucrose-treated controls. The difference became greater after longer ethanol treatment and larger doses. However, when threshold measurements were made under the acute influence of ethanol in the experimental group, the mean values were virtually equal to those of the sucrose controls. This normalization, by ethanol, of a disturbance produced by absence of ethanol in a chronically treated animal is indicative of physical dependence. Following termination of ethanol treatment there was a gradual return of startle thresholds almost to control values over a relatively short period, indicating that the changes underlying the hyperexcitability are readily reversible.
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    URL: http://dx.doi.org/10.1007/BF00402633
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    Untersuchungen über die Stereotypien nach Amphetamin und Apomorphin sowie deren pharmakologische Beeinflussung (1971)
    Springer
    Psychopharmacology 21 (1971), S. 212-228 
    Details
    ISSN: 1432-2072
    Keywords: Amphetamine ; Apomorphine ; Stereotypy ; Behaviour ; Rat ; Mouse ; Neuroleptics ; Thymoleptics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The behaviour of mice after various doses of amphetamine or apomorphine which induce stereotypies is described in details. The induced stereotyped behaviour in mice appears more differentiated than that in rats. The amphetamine-syndrome in rats consists of an excitement phase followed by the stereotypy, whereas apomorphine elicits stereotyped behaviour directly after administration. Amphetamine-induced stereotyped behaviour is different from the apomorphine-induced stereotypy in several respects. Neuroleptics inhibit the amphetamine (12.5 mg/kg s.c.) and apomorphine-(10 mg/kg s.c.) syndrome, while thymoleptics potentiate subeffective doses of amphetamine (5 mg/kg s.c.) and apomorphine (2 mg/kg s.c.). This is demonstrated with some neuroleptics of the phenothiazine-type, haloperidol, reserpine, and a group of thymoleptics. The method opens the possibility of differentiating within several groups of neuroleptics and within several groups of thymoleptics.
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    URL: http://dx.doi.org/10.1007/BF00403860
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    The effect of two tetrahydrocannabinols, (Δ9-THC and Δ8-THC) on conditioned avoidance learning in rats and its transfer to normal state conditions (1971)
    Springer
    Psychopharmacology 22 (1971), S. 23-30 
    Details
    ISSN: 1432-2072
    Keywords: Acquisition ; CAR ; Tetrahydrocannabinols ; Transfer ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Rats trained in conditioned avoidance responding (CAR) after injections of either 7.5 mg/kg Δ 9-THC (tetrahydrocannabinol) or 15 mg/kg Δ 8-THC, showed no transfer when tested in the non-drugged state. Furthermore, these doses of the isomeric tetrahydrocannabinols exerted a disruptive effect on previously established CAR in rats, trained under normal conditions. Only the Δ 9-THC-group showed an impairment of acquisition which was statistically significant compared to the control group.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00401463
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    The effects of cholinergic agents upon fixated behavior (1971)
    Springer
    Psychopharmacology 21 (1971), S. 147-156 
    Details
    ISSN: 1432-2072
    Keywords: Scopolamine ; Pilocarpine ; Lashley Jumping Stand ; Fixated Behavior ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract A group of male albino rats were subjected to the Maier paradigm (insoluble problem followed by a soluble problem) using the Lashley jumping stand. Forty-two animals which failed the soluble problem by adopting a position stereotype were then randomly assigned to eight drug groups in a 2×2 design. Animals were guided to the correct window on odd days but received no guidance on even days. Animals received either pilocarpine nitrate (5.0 mg/kg) or scopolamine hydrobromide (1.0 mg/kg) in one of three different sequences. These sequences included drug on both odd and even days (drug-drug), only on the odd day (drug-no drug), or only on the even day (no drug-drug). One other drug group received scopolamine methylbromide (1.0 mg/kg) in a drug-no drug sequence, while the control group received saline on both days. Results indicated that animals receiving pilocarpine in the drug-drug and no drug-drug sequence solved significantly faster than the controls, while all the drugno drug groups showed significantly poorer solution rates. It was concluded that pilocarpine may enable animals to inhibit punished behavior patterns and thus hasten the extinction of fixated responses, but that due to the inconclusive scopolamine data this pilocarpine effect may not be due to its cholinomimetic properties.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00572272
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    Biochemical evidence of adrenergic interaction with cholinergic function in the central nervous system of the rat (1971)
    Springer
    Psychopharmacology 21 (1971), S. 238-246 
    Details
    ISSN: 1432-2072
    Keywords: NE ; Intraventricular ; Cholinergic Function ; Eating Behaviour ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The effect of centrally administered norepinephrine (NE) into the lateral ventricle on choline acetylase activity (ChAc), endogenous levels of NE, dopamine (DA) and serotonin (5HT) was studied in different regions of the rat brain. In normal rats, the highest activity of choline acetylase was found in the cerebral cortex, followed by the brain stem, the diencephalon, the hypothalamus and least in the cerebellum. Chronic administration of NE significantly increased the choline acetylase activity in all regions studied, whereas both the acute and the in vitro studies showed no significant change except in the brain stem. Serotonin level was increased in the cerebellum, but decreased in the diencephalon and the brain stem. There was no significant alteration in the level of NE in all areas studied except in the hypothalamus where there was an increase in the mean concentration. Tissue level of DA showed a significant increase in the cerebral cortex and the hypothalamus. Behaviourally, there was a significant increase in food intake on the first day of treatment with no significant change in water intake. The data suggests that NE may be involved in the regulation of acetylcholine synthesis. The significance of interaction between different monoamines and acetylcholine may be important in the study of drug tolerance phenomena.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00403862
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    Role of γ-aminobutyric acid (GABA) in the extrapyramidal motor system 1. Regional distribution of GABA in rabbit, rat, guinea pig and baboon CNS (1971)
    Springer
    Experimental brain research 13 (1971), S. 514-518 
    Details
    ISSN: 1432-1106
    Keywords: GABA-Regional distribution ; Rabbit ; Rat ; Guinea pig ; Baboon ; CNS ; Substantia nigra
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The regional distribution of GABA in the CNS of rabbit (17 regions), rat (14 regions), guinea pig (12 regions) and baboon (11 regions) was determined by sensitive enzymatic and fluorometric assay. In all species studied a high concentration of GABA was found in substantia nigra (8.5–10.1 mmoles/kg) and pallidum (6.5–8.2 mmoles/kg). Fairly high amounts were found in hypothalamus, superior and inferior colliculi, nucleus oculomotorius and dentate nucleus. The GABA concentration in spinal cord (white matter) had the lowest value.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00234282
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    NaK stimulated adenosinetriphosphatase: Intracellular localisation within the proximal tubule of the rat nephron (1971)
    Springer
    Pflügers Archiv 330 (1971), S. 265-270 
    Details
    ISSN: 1432-2013
    Keywords: NaKATPase ; Intracellular Localisation ; Proximal Tubule ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary With refinements of quantitative histochemistry, i.e. oilwell technique, enzymaticP ianalysis and NADP/NADPH cycling an enzymatic polarization of the tubular epithelial cell from the rat nephron to NaKATPase can be established. NaKATPase activity is limited to the basal area of the epithelial cell. Brush border fragments lack NaKATPase activity.
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    URL: http://dx.doi.org/10.1007/BF00588617
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    Influence de l'isolement sur le comportement d' agressivité intraspécifique induit par l'apomorphine chez le rat (1971)
    Springer
    Psychopharmacology 20 (1971), S. 389-394 
    Details
    ISSN: 1432-2072
    Keywords: Aggressive Behaviour ; Apomorphine ; Isolation ; Psycho pharmacology ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The apomorphine-induced aggression response of isolated rats was examined. The development of aggressive behaviour in adult rats was enhanced after isolation in cages with wire-netting and especially in cages isolated in opacified Makrolon boxes compared with aggregated rats. Young rats kept isolated for 2 months immediately after weaning showed an enhancement only if isolated in wire-netting cages. Rats kept in groups for one month after their isolation still showed an enhancement of aggression, although it was somewhat reduced.
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    URL: http://dx.doi.org/10.1007/BF00403570
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    Central action of phentolamine administered intraventricularly in the rat (1971)
    Springer
    Psychopharmacology 20 (1971), S. 348-354 
    Details
    ISSN: 1432-2072
    Keywords: Phentolamine ; Noradrenaline ; Amphetamine ; Rat ; Locomotor Activity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Phentolamine injected intraventricularly in unanaesthetized rats decreased their locomotor activity and antagonized the excitatory effect of intraventricularly injected noradrenaline, or amphetamine injected s.c. Phentolamine did not influence the level of noradrenaline and 5-hydroxytryptamine in the rat brain. The mechanism of observed action of phentolamine is discussed.
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    URL: http://dx.doi.org/10.1007/BF00403566
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    The effects of acutely administered fenfluramine on activity and eating behaviour (1971)
    Springer
    Psychopharmacology 21 (1971), S. 165-173 
    Details
    ISSN: 1432-2072
    Keywords: Anorexic Effect ; Fenfluramine ; Activity Analysis ; Time Sampling Technique ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Three experiments are reported investigating the effects of acute administration of fenfluramine over 3 doses on activity, eating and drinking behaviour. A time sampling procedure of activity analysis was used, employing six behaviour categories. Fenfluramine produced a dose related decrease in rearing behaviours, with some evidence of an increase in walking categories at the lower dose levels. Eating and drinking behaviours showed clear dose related decreases. Sniffing categories showed a clear dose related increase. Attention is drawn to some of the difficulties of interpretation and assessment of anorexic effect.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00572274
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    The influence of 1-(o-Allylphenoxy)-3-Isopropylamino-2-Propanol Hydrochloride (Alprenolol) on the central nervous system of the rat (1971)
    Springer
    Psychopharmacology 21 (1971), S. 66-73 
    Details
    ISSN: 1432-2072
    Keywords: Alperenolol ; Amphetamine ; Brain Amines ; Behaviour ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The influence of alprenolol and amphetamine alone or combined on the content of noradrenaline, dopamine and 5-hydroxytryptamine in seven discrete areas of the brain and on rat behaviour was studied. Animals were treated with drugs for 6 months. Alprenolol caused mainly a decrease of the estimated endogenous amines in different brain areas. Amphetamine caused a decrease of all three amines in some parts of the brain, and reversed some of the changes caused by alprenolol. Alprenolol had no effect on the locomotor activity of rats, but increased the activity of rats treated with amphetamine after the first week of treatment, and antagonized the excitatory effect caused by amphetamine during the following weeks of the experiment.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00403997
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    Der Einfluß der Durchblutung auf die Resorption von Polyalkoholen aus dem Jejunum der Ratte (1971)
    Springer
    Naunyn-Schmiedeberg's archives of pharmacology 270 (1971), S. 22-40 
    Details
    ISSN: 1432-1912
    Keywords: Intestinal Blood Flow ; Intestinal Absorption ; Polyalcohols ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary 1. Jejunal loops of anaesthetized rats were perfused with14C-labelled polyalcohols in buffered saline solutions at pH 7. The blood flow, the disappearance rate (from the intestinal lumen), and the appearance rate (in the intestinal venous blood) were determined simultaneously. 2. A decrease of the blood flow from about 1.8 to 0.25 ml min−1g−1 wet tissue diminished the disappearance and appearance rate of the substances and their possible metabolites. An increase of the blood flow caused the reversed results. The two transfer rates and their dependence on blood flow decreased with increasing molecular weight of the polyalcohols. 3. The appearance rate of glycerol showed a different dependence on blood flow in experiments with increasing and decreasing blood flow. This phenomenon can be explained by a decreasing permeability of the epithelium for glycerol with time. The cause for it is unknown. 4. By means of a three-compartment-model blood flow independent permeability coefficients of the polyalcohols are derived for the intestinal epithelium. They are consistent with an equivalent pore radius of 3.9±0.1 Å.
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    URL: http://dx.doi.org/10.1007/BF00997297
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    On the innervation of the rat and pig adrenal cortex (1971)
    Springer
    Cell & tissue research 116 (1971), S. 151-156 
    Details
    ISSN: 1432-0878
    Keywords: Adrenal cortex ; Innervation ; Receptors ; Rat ; Pig
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The innervation of the adrenal cortex of the rat and the pig is investigated with the electron microscope. Nerve fibers containing synaptic and two types of dense-cored vesicles come into contact with endocrine cells. There are no specialized pre- and postsynaptic membranes. The synaptic cleft is about 200 Å wide. Generally the basement membrane between nerve and cell is absent. These observations are discussed on the base of more recent experimental findings. Small fibers having an average diameter of about 0.2 to 0.5 μ and containing only tubules and filaments are considered to represent parts of an afferent nervous system.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00332863
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    Histochemical and chemical studies on pre- and postnatal development of the different systems of “short” and “long” adrenergic neurons in peripheral organs of the rat (1971)
    Springer
    Cell & tissue research 116 (1971), S. 319-341 
    Details
    ISSN: 1432-0878
    Keywords: Adrenergic neurons ; “Short” and “long” systems ; Pre- and postnatal development ; Peripheral organs ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The adrenergic innervation in the submaxillary gland, heart, kidney, small intestine, and accessory male genital organs and the development of the adrenal chromaffin cells and the sympathetic ganglia were studied in the rat from 15 days post coitum to 16 days post partum using the fluorescence histochemical method of Falck and Hillarp. The postnatal development of the noradrenaline concentrations in the heart and vas deferens was followed by fluorometric determinations. At about 15 days post coitum, the anlagen of the sympathetic chains were well visible in the form of two dorsal segmented columns of small branching sympathicoblasts exhibiting an intense catecholamine fluorescence. In the midline, ventrally to these two anlagen, another column of sympathicoblasts developed; this seemed to give rise to the prevertebral ganglia and to the short adrenergic neurons supplying the internal genital organs. At the level of the adrenal anlagen, small intensely fluorescent chromaffin cells were collected in two bilateral groups which became enclosed by adreno-cortical cells. This enclosure was, however, not complete even at two weeks post partum. Bundles of growing sympathetic nerves were visible in the periphery of the various organs studied at 19–21 days post coitum. A terminal innervation of the organs suggestive of a functional transmitter mechanism did not start to establish until at or immediately after birth. The final pattern of innervation was usually reached at about one week post partum, and the following development proceeded largely in the form of a quantitative increase in the number of nerves participating in the innervation apparatus. The adult level of noradrenaline in the heart and vas deferens was reached three to five weeks after birth. The small intestine was an exception in that the final pattern of innervation in the wall was attained immediately after birth. There was no overt difference in the rate of development of the terminal sympathetic innervation in organs supplied by short adrenergic neurons (accessory male genital organs) compared to the innervation of the submaxillary gland, heart and kidney, which receive classical long adrenergic neurons.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00330631
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    The fine structure of the granulosa cells in the domestic fowl and the rat (1971)
    Springer
    Cell & tissue research 119 (1971), S. 58-67 
    Details
    ISSN: 1432-0878
    Keywords: Ovary ; Rat ; Fowl ; Granulosa cells ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The granulosa cells of the ovarian follicle of the rat and the domestic fowl have been studied with the light and electron microscope. The nuclei of the granulosa cells were irregular with indentations and large in proportion to the cytoplasm of the cell. The mitochondria had a dense, dark matrix with only few cristac. The Golgi apparatus was moderately developed, located towards the oocyte in a juxtanuclear position. The endoplasmic reticulum was rather sparse. Lipid droplets were only occasionally encountered. Microtubules were regularly observed. The functions of the granulosa cells are discussed. Compared with the steroid-producing cells of the theca interna of the same follicles, the granulosa cells primarily are the nursing cells for the growing oocyte and mainly have the characteristics of protein forming cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00330538
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    Ultrastructural studies on the hypothalamic neurosecretory neurones of the rat (1971)
    Springer
    Cell & tissue research 122 (1971), S. 283-300 
    Details
    ISSN: 1432-0878
    Keywords: Paraventricular nucleus ; Rat ; Synthesis of hormones ; Acid phosphatase ; Osmium impregnation ; Neurosecretion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The ultrastructural features of the paraventricular neurones of the non-treated rat are presented comparing them with those of the supraoptic neurones. No striking differences are seen between the general electron microscopic characteristics of the paraventricular and supraoptic neurones. The importance of adequate fixation to obtain good preservation of the neurones is emphasized, since inadequate fixation can cause e.g. artefactual appearance of “dark neurones”. The previously presented classification of the neurosecretory neurones into two categories (e.g. “light and dark neurones”) on the basis of the number of ribosomes is not considered justifiable, since their number can vary to a very great extent even within a single cell. The synthesis of neurosecretory products in the paraventricular neurones obviously follows the general mode of the synthesis of secretory proteins: ribosomes—RER—Golgi complex—secretory vesicles. On the basis of the localization of heavy metal deposits after osmium impregnation and demonstration of acid phosphatase the Golgi complex of the paraventricular neurones is found to be polarized. The direction of the polarity is discussed. The substructures of the dense cores of the neurosecretory granules and of the contents of the lysosomal dense bodies are nearly identical. Therefore it is considered impossible to determine positively the nature of the dark condensed material within the Golgi complex. The characteristics of the immature neurosecretory granules and the possibility of releasing neurosecretory products into the cytoplasm already within the perikarya are speculated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00935990
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    A two-filament system and interaction of heavy meromyosin (HMM) with thin filaments in smooth muscle (1971)
    Springer
    Cell & tissue research 122 (1971), S. 350-356 
    Details
    ISSN: 1432-0878
    Keywords: Smooth muscle ; Two-filament system ; Small intestine ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The structure of glycerinated smooth muscle from small intestine of adult rat was investigated by electron microscopy. In the central parts of the tissue blocks a two-filament system was found, consisting of parallel thick and thin filaments with regularly spaced interconnections, closely resembling that of striated muscle. In the peripheral parts of the blocks only thin filaments were found. The thin filaments were identified as actin by the formation of arrowhead complexes after incubation with heavy meromyosin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00935994
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    Identification of helical polyribosomes in sections of mature skeletal muscle fibers (1971)
    Springer
    Cell & tissue research 121 (1971), S. 531-547 
    Details
    ISSN: 1432-0878
    Keywords: Helical polyribosomes ; Skeletal muscle ; Polysomes ; Levator ani ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The localization and configurations of ribosomes in mature white skeletal muscle fibers of the rat were investigated. Differential visualization of ribosomes and glycogen granules was obtained by fixation with glutaraldehyde only and staining of the sections with uranyl acetate. Ribosomes are then electron dense and glycogen granules electron transparent. Their identity was ascertained by selective extractions of ribonucleic acid and polysaccharide. The vast majority of the ribosomes is not membrane-bound. They are located intermyofibrillarly (predominantly at the level of the I-bands), beneath the sarcolemma, and in the paranuclear cones of sarcoplasm. Occasionally short stretches of granular reticulum occur, often as characteristic double walled vesicles with ribosomes on the inner membrane only. Three main types of polysomal configurations are observed: rosettes of 4 to 6 ribosomes, helical arrays, and whorls of up to about 25 probably membrane-bound ribosomes. The average number of ribosomes in the extended helical configurations is estimated to be about 60. It is argued that these helices represent the polysomes instrumental in the synthesis of the large subunits of myosin. It is emphasized that helical polyribosomes are by no means typical of striated muscle, but probably represent a common configuration of large free polysomes.
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    URL: http://dx.doi.org/10.1007/BF00560158
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    Licht- und elektronenmikroskopische Untersuchungen über die Reversibilität von Kernmembranschrumpfungen des Ependyms im Hypothalamus der Ratte, hervorgerufen durch langdauernde Thiobarbiturat-Narkose (1971)
    Springer
    Cell & tissue research 112 (1971), S. 542-550 
    Details
    ISSN: 1432-0878
    Keywords: Ependyma ; Thiobarbiturate narcosis ; Cell nucleus shrinkage ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Im Ependym des Hypothalamus wurde das Verhalten der Zellkern-Membran nach ausgedehnter intraperitonealer Thiobarbiturat-Narkose untersucht. Die Kernmembran ist gefaltet, der Zellkern zeigt Zeichen von Schrumpfung. Diese Veränderung ist reversibel und wird nicht von der mit der Narkose verbundenen Entwässerung verursacht. Elektronenmikroskopische Untersuchungen lassen einen normalen Stoffwechsel vermuten, Zeichen von Pyknose fehlen.
    Notes: Summary Effects on the nuclear membrane were investigated in hypothalamic ependymal cells of the rat during prolonged intraperitoneal thiobarbiturate narcosis. The nuclear membrane of the nucleus was folded and the nucleus showed signs of shrinkage. These alterations were reversible and did not depend on the dehydration which developed during the narcosis. Ultrastructural signs and the absence of pyknosis suggested that metabolism during nuclear shrinkage may be normal.
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    URL: http://dx.doi.org/10.1007/BF01033379
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    Ultrastructural observations on the postnatal development of the rat prostate (1971)
    Springer
    Cell & tissue research 113 (1971), S. 157-173 
    Details
    ISSN: 1432-0878
    Keywords: Prostate ; Development ; Ultrastructure ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The postnatal development of the rat prostate has been studied with the electron microscope. Major developmental changes begin during the second week after birth and involve organelles associated with the formation of secretions. The amount of granular endoplasmic reticulum and the size of the Golgi complex increase greatly. Large vacuoles that probably contain secretory material are formed, and the lumen of the prostatic acini appears to contain secreted material. Large lysosomes with polymorphic interiors are present as early as 10 days after birth, and they become numerous by the end of the third week. Differences in fine structure between the different lobes of the prostate are detectable in 10–14 day old rats. The subsequent differentiation of the granular endoplasmic reticulum into the forms characteristic of the different prostatic lobes is described. The initial changes in the prostate occur in advance of sexual maturity of the animal, and the adult appearance of the gland is attained by 4–5 weeks after birth.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00339413
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    An ultrastructural study of the neurosecretory cells of the medial vascular prechiasmatic gland, the preoptic recess and the anterior part of the suprachiasmatic area (1971)
    Springer
    Cell & tissue research 114 (1971), S. 404-440 
    Details
    ISSN: 1432-0878
    Keywords: Neurosecretory cells ; Rat ; Cytoplasmic bodies resembling nucleoli ; Paranucleolar and coiled bodies
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A distinctive cytoplasmic inclusion, termed nucleolus-resembling body, was identified in the neurons, but not in the glial cells, of the medial vascular prechiasmatic gland, the preoptic recess and the anterior part of the suprachiasmatic area in the rat. This ultrastructure which lacks a limiting membrane is made of an entanglement of tightly packed filaments, 60–70 Å in diameter. Each filament consists of smaller units, 15–20 Å thick. Usually, only one such ultrastructure is present in a cell, but at time two were identified. It is present in most neurosecretory cells. It is surrounded by polyribosomes in the perikaryon of the nerve cells. Furthermore, it is linked to peripherally located ribosomes by means of filaments, 70 Å in diameter. A possible function in protein synthesis of structural components, such as neurofilaments, is hypothesized. Two distinctive fibrillar areas were identified in the nucleolus according to the tightness with which fibrils were packed together. Their significance is discussed. Nucleolar extrusions were never observed in our studies. Paranucleolar and coiled bodies were identified in the nuclear sap. The mechanism of formation and the significance of these bodies are discussed.
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    URL: http://dx.doi.org/10.1007/BF00331464
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    Long-term trophic effect of ACTH on rat adrenocortical cells (1971)
    Springer
    Cell & tissue research 115 (1971), S. 30-45 
    Details
    ISSN: 1432-0878
    Keywords: Adrenal Cortex ; ACTH ; Autoradiography ; Stereology ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The changes occurring in rat adrenocortical cells (zona fasciculata) during an 8 day period of treatment with ACTH, were investigated by morphometric and autoradiographic methods. The most important ultrastructural change consists in a conspicuous increase in the smooth endoplasmic reticulum, that accounts for about 50% of the total increase of cellular volume. Also the mitochondrial fraction shows a significant increase, which is found to be due both to the increment in the number of mitochondria per cell and to the increase in the mean volume of organelles themselves. The quantitative autoradiographic data, indicating an increment in the incorporation of 3H-orotate and 3H-leucine into adrenocortical cells of the treated animals, allow us to conclude that the ACTH-induced ultrastructural changes are the morphological expression of a stimulation of the cellular protein synthesis. Since mitochondria are largely autonomous in the synthesis of their enzymes and structural proteins, it is possible to hypothesize that ACTH also intervenes in the regulation of the mitochondrial protein synthesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00330212
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    Sur l'existence de cellules de type ≪corticotrope≫ dans la pars intermedia de l'hypophyse du rat (1971)
    Springer
    Cell & tissue research 115 (1971), S. 60-68 
    Details
    ISSN: 1432-0878
    Keywords: Adenohypophysis ; Pars intermedia ; Corticotrophic cells ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Résumé Nos observations démontrent l'existence de deux types de cellules glandulaires dans la pars intermedia de l'hypophyse du rat: des cellules propres, à M.S.H., dont les grains de sécrétion sont détruits par la fixation osmiée et relativement bien conservés par la fixation au glutaraldéhyde; des cellules à petits grains denses, osmio-résistants, qui s'apparentent morphologiquement aux éléments corticotropes de l'antéhypophyse. L'existence bien connue d'A.C.T.H. ou, plus précisément, d'un facteur ≪A.C.T.H.-like≫ dans le lobe neuro-intermédiaire nous fait considérer comme très vraisemblable la nature corticotrope de ce deuxième type cellulaire de la pars intermedia.
    Notes: Summary Two types of glandular cells have been shown to occur in the intermediate lobe of the rat hypophysis: 1) M.S.H. producing cells whose secretory granules are destroyed by osmium fixation and relatively well preserved by glutaraldehyde fixation, 2) cells with small dense osmium resistant granules being morphologically similar to the corticotrophic cells of the anterior lobe. The well known occurrence of A.C.T.H. or, more precisely, of an “A.C.T.H.-like” factor in the neuro-intermediate lobe makes the corticotrophic nature of this second cell type very likely.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00330214
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    Neurofilaments and microtubules in sensory neurons after peripheral nerve section (1971)
    Springer
    Cell & tissue research 117 (1971), S. 191-211 
    Details
    ISSN: 1432-0878
    Keywords: Sensory neurons ; Rat ; Nerve section ; Neurofilaments ; Neurofibrils
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Sensory neurons were examined in spinal ganglia of the rat 1 to 55 days after section of the plexus brachialis nerves. Only “light” neurons of the type A were investigated. Maximal reaction to axotomy was found 7 to 14 days after the operation. The majority of the axotomized perikarya developed central chromatolysis. In such neurons, Nissl bodies virtually disappeared from the central area of the neuron and formed a more or less continuous zone at the cell circumference. The cytocentrum became filled with large numbers of mitochondria, dense bodies and other organelles. Neurofilaments and microtubules were disarranged and ran at random among the accumulated particles. Microtubules were often more prominent in chromatolytic areas than neurofilaments. Both these organelles were rare in the peripheral areas filled with massed Nissl substance. Part of the neurons that did not show typical chromatolysis contained increased numbers of neurofilaments among Nissl bodies dispersed throughout the cytoplasm. Neurofilaments were roughly arrayed in bundles up to several microns wide; they were linked by cross-bridges and separated by distances of about 500 Å. Microtubules were rarely found in the filamentous areas. However, they were numerous in the axon hillock and in the initial segment where they formed fascicles similar to those described in normal neurons of other types. During the period from 14 to 55 days after axotomy, many perikarya recovering from chromatolysis contained enlarged bundles of neurofilaments with occasional microtubules among the restored Nissl bodies. Mean diameters of sensory neurons, measured 7 to 55 days after axotomy, in no instance exceeded those of contralateral control neurons. It thus appears that sensory perikarya do not increase in size either during the chromatolytic process or during the period of recovery.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00330737
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    Geschlechtsunterschiede in der Ultrastruktur der Nebennierenrinde der Ratte (1971)
    Springer
    Cell & tissue research 116 (1971), S. 136-150 
    Details
    ISSN: 1432-0878
    Keywords: Adrenal cortex ; Rat ; Sex difference ; ACTH ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Die Nebennierenrinde von 178 Sprague-Dawley-Ratten wurde elektronenmikroskopisch von der 1.–12. Lebenswoche unter Normalbedingungen sowie nach s.c. Injektion von 3 IE ACTH/100 g Körpergewicht innerhalb von 2 min — 24 Std untersucht. Ab der 5. Lebenswoche unterscheiden sich die Nebennieren von Weibchen im Bereich der äußeren Zona fasciculata durch Kernvergrößerung, größere Mitochondrien und kleindisperse Liposomen von denen der Männchen. Zyklusbedingte Schwankungen in der Ultrastruktur der weiblichen Nebennierenrinde bestehen nicht. Nach ACTH-Applikation nähert sich das Nebennierenbild des Männchen innerhalb von 30 min dem des unbehandelten Weibchens. Außerdem kommt es bei beiden Geschlechtern durch ACTH zu einer Dispersion und Reduktion der Liposomen, Vergrößerung des Golgiapparates und der Zellkerne, Ausweitung des endoplasmatischen Retikulums und Vermehrung der Mikrovilli. Der Geschlechtsdimorphismus wird funktionell erklärt.
    Notes: Summary 178 Sprague-Dawley-rats were studied by electron microscopy from the 1st to the 12th week of life under normal conditions and after s. c. injection of 3 I. U. ACTH/100 gbodyweight from 2 min to 24 hours. Beginning with the 5th week of life females differ from males by small dispersed liposomes and enlarged nuclei and mitochondria in the outer z. fasciculata. There is no change in the ultrastructure of the female adrenal cortex depending on the sexual cycle. After application of ACTH the adrenal cortex of males assimilates to the untreated females within 30 min. After ACTH-application both sexes show dispersion and reduction of liposomes, dilation of endoplasmatic reticulum, increasement of microvilli and enlargement of the Golgi apparatus. The sex-dimorphism is interpreted by functional differences.
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    URL: http://dx.doi.org/10.1007/BF00332862
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    Wachstumsverhältnisse des Samenblasenepithels bei der heranwachsenden weißen Ratte (1971)
    Springer
    Cell & tissue research 119 (1971), S. 378-383 
    Details
    ISSN: 1432-0878
    Keywords: Seminal vesicles ; Rat ; Epithelium ; Proliferation activity ; Autoradiography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung An 12 männlichen weißen Wistarratten unterschiedlichen Alters wurde autoradiographisch die Proliferation des Samenblasenepithels zur Zeit der Pubertät geprüft. Die Tiere waren 21, 29, 40, 50, 60 und 180 Tage alt und erhielten 1 Std vor der Tötung durch Dekapitation 2,5 μC 3H-Thymidin/g Körpergewicht i. p. appliziert. Ausgewertet wurden Autoradiogramme nach einer Expositionszeit von 18 Tagen; bestimmt wurde der Prozentsatz der markierten Zellkerne (3H-Index). Im Gegensatz zu dem bekannten exponentiellen Abfall des 3H-Indexes bei nicht-hormonabhängigen Leberepithelien jugendlicher Ratten kommt es beim Epithel der Samenblasen um den 40. Lebenstag zu einem erneuten, starken Proliferationsschub. Diese starke Wachstumszunahme ist wahrscheinlich der proliferationskinetische Ausdruck der nach Steinberger (1970) zur gleichen Zeit stattfindenden hormonellen Umschaltung in den Testes. Anschließend geht das Vermehrungswachstum des jugendlichen Tieres, durch das die Zellzahl erhöht wird, in das Erhaltungswachstum über; der 3H-Index des Samenblasenepithels erreicht den Wert des erwachsenen Tieres.
    Notes: Summary In 12 male white rats (Wistar) the proliferative activity of the epithelial cells in the seminal vesicles was studied by autoradiography. The animals were 21, 29, 40, 50, 60 and 180 days old. Each animal was given 2.5 μC/g 3H-thymidine and was killed 1 hour thereafter. We analysed the autoradiograms recording the percentage of labelled nuclei (3H-index). While the 3H-index of the non hormone-dependent epithelial cells in the liver of young rats declines continously with growing age (Stocker et al., 1964), the epithelial cells in the seminal vesicles of 40 days old rats show a remarkable rise in their proliferative activity. It is quite possible that this new peak in the proliferation is the morphological result of a preceeding switch in hormonal production of the testes as reported by Steinberger (1970). Thereafter the growth proliferation of the young rats declines into the steady state of the grown up.
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    URL: http://dx.doi.org/10.1007/BF00431294
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    Untersuchungen zur Fettresorption im Dünndarm der Ratte, unter besonderer Berücksichtigung der Tagesrhythmik (1971)
    Springer
    Cell & tissue research 119 (1971), S. 334-354 
    Details
    ISSN: 1432-0878
    Keywords: Fat absorption ; Small intestine ; Rat ; Micropinocytosis ; Circadian rhythm
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Die vorliegende Untersuchung will klären, welche Rolle die Mikropinocytose bei der Fettresorption spielt und ob die Fettresorption tagesrhythmische Schwankungen aufweist. Zu diesem Zweck wurde Fett zu verschiedenen Tages- und Nachtzeiten in abgebundene Jejunumabschnitte von Ratten injiziert, das Darmgewebe in verschiedenen Abständen licht- und elektronenmikroskopisch untersucht und die Zahl der mikropinocytotischen Invaginationen der Enterocyten bestimmt. Folgende Resultate wurden erzielt: Die Häufigkeit der Mikropinocytose (mikropinocytotische Invaginationen/100 Mikrovilli) beträgt bei Hungertieren 4,78, bei Normaltieren 5,06, 1 min nach Gabe von Mazolachymus 3,55, 1/16 m NaCl-Lösung 4,37, Butterschmalz 5,50, Rindertalg 7,16, Schweineschmalz 7,66 und Mazola 9,25; 2 1/2 min nach Mazolagabe 8,23, nach 5 min 7,67, nach 10 min 6,76. Zwischen 10 und 15 min wird der Ausgangswert erreicht. Aufgrund statistischer Auswertung der Befunde wird geschlossen, daß Fett vor allem in den Frühstadien der Fettresorption durch Mikropinocytose aufgenommen werden könnte. Ferner zeigt sich, daß die Mikropinocytosehäufigkeit entscheidend von der chemischen Zusammensetzung und dem physikalischchemischen Zustand der verabreichten Fette beeinflußt werden dürfte. — Lichtmikroskopisch wurde nachgewiesen, daß die Fettresorption charakteristische, tagesrhythmische Schwankungen aufweist. Um 2.00 Uhr ist sie am stärksten, um 14.00 Uhr am geringsten. Es wird diskutiert, worauf die tagesrhythmischen Unterschiede zurückzuführen sind.
    Notes: Summary The present study attempts to elucidate (a) to what extent micropinocytosis is involved in the process of fat absorption and (b) whether fat absorption shows a circadian rhythm. For this purpose rats were given 0.1 to 0.15 ml of fat directly into the upper jejunum at various times of the day and night. 20 sec to 20 min after administration of fat small pieces of the jejunum were rapidly dissected out, the tissue examined by means of light- and electron microscopy, and the number of micropinocytotic pits occurring in the enterocytes during fat absorption was determined. The following results were obtained: The frequency of micropinocytosis (micropinocytotic pits/100 microvilli) is as follows: in starved rats 4.78, in normal rats 5.06, 1 min after administration of corn oil chyme 3.55, 1/16 m NaCl-solution 4.37, clarified butter 5.50, ox tallow 7.16, lard 7.66, corn oil 9.25; 2 1/2 min after administration of corn oil 8.23, after 5 min 7.67, and after 10 min 6.76. Between 10 and 15 min the number of micropinocytotic pits reverses to normal. From statistical analysis of the results it is concluded that micropinocytosis can play a significant role in the early stages of fat absorption. The experiments show in addition that micropinocytosis is greatly influenced by the chemical composition and the physico-chemical state of the administered fat.—Light microscopic studies indicate that fat absorption varies considerably during the 24 hr period. The highest absorption was found at 2 a.m., the lowest at 2 p.m.
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    URL: http://dx.doi.org/10.1007/BF00431292
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    Light and electron microscopic autoradiography of substantia nigra of rat after intraventricular administration of tritium labelled norepinephrine, dopamine, serotonin and the precursors (1971)
    Springer
    Cell & tissue research 115 (1971), S. 137-148 
    Details
    ISSN: 1432-0878
    Keywords: Substantia nigra ; Rat ; Incorparation of 3H-Dopamine, 3H-Serotonin and 3H-Norepinephrine ; Axo-dendritic Synapses ; Autoradiography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In autoradiographies of substantia nigra in rat, it has been observed that after intraventricular injections of 3H-dopamine and 3H-norepinephrine respectively the silvergrains are accumulated in nigra neurons and their dendritic branches. The incorporation was more pronounced in the case of 3H-norepinephrine than 3H-dopamine. This seems to indicate that exogenous norepinephrine may have stronger affinity to nigra neurons and their dendrites than exogenous dopamine. In addition, some 3H-dopamine and 3H-norepinephrine labelled nerve terminals were observed in axo-dendritic synapses. In contrast to these data, 3H-5HTP and 3H-5HT administration showed almost all silver grains accumulated in the neuropil when observed in light microscopic autoradiography. Electron micrographs further reveal that the incorporation of 3H-5HTP and 3H-5HT was mostly within axo-dendritic boutons with more frequent dense core vesicles. These data again strongly suggest that substantia nigra receives a large number of serotoninergic fibres forming axo-dendritic synapses which may play an important role in modulation of substantia nigra function.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00330222
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    Vergleichende elektronenmikroskopische Untersuchung an entodermalen Thymus-Retikulumzellen neugeborener und alter Wistar-Ratten (1971)
    Springer
    Cell & tissue research 114 (1971), S. 271-280 
    Details
    ISSN: 1432-0878
    Keywords: Thymus ; Rat ; Involution ; Reticulum-Cells ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung 1. Entodermale Thymus-Retikulumzellen neugeborener Wistar-Ratten enthalten häufig helle Vesikel mit enger räumlicher Beziehung zum Golgi-Apparat. Bei 13 Monate alten Ratten fehlen diese Vesikel. 2. Die Tatsache, daß die Häufigkeit heller Vesikel mit fortschreitendem Lebensalter abnimmt, findet ihre Parallele in der von anderer Seite getroffenen Feststellung, daß die Bildung eines humoralen Faktors im Thymus im Laufe der Zeit eingeschränkt wird. 3. Diese Koinzidenz läßt an die Möglichkeit denken, die hellen Vesikel könnten ein morphologisches Äquivalent der Bildung eines Thymuswirkstoffes sein. 4. In entodermalen Thymus-Retikulumzellen 13 Monate alter Ratten finden sich große Vakuolen mit granulärem Inhalt. Ihre Bildung beginnt schon vor der Thymusinvolution. Sie entstehen wahrscheinlich durch Phagozytose oder Aufnahme zelleigenen Materials, das nicht weiter abbaufähig ist. 5. Da diese Einschlüsse mit fortgesetzem Alter an Zahl und Größe zunehmen, scheinen sie als Ausdruck einer Beeinträchtigung der Stoffwechselvorgänge im Zytoplasma ein erstes morphologisch faßbares Symptom der frühzeitigen physiologischen Involution des Thymus zu sein.
    Notes: Summary 1. Entodermal reticulum cells of new born Wistar rats frequently contain clear vesicles with close connection to the Golgifield. These vesicles do not occur in reticulum cells of 13 months old rats. 2. The frequency of these clear vesicles decreases with advancing age obviously in the same way as the production of a humoral thymic factor reported by several investigators. 3. This observation agrees with the hypothesis that the clear vesicles are the morphological equivalent of the production of a humoral thymus factor. 4. Entodermal reticulum cells of 13 months old Wistar rats contain large vacuoles filled with electron dense, granulated materials. The formation of these vacuoles starts already before the beginning of the thymus involution. Possibly they are produced by phagocytosis or necrobiosis and contain deposits of material that cannot undergo further degradation. 5. The increase of these vacuoles in number and size with advancing age may be considered as equivalent of the deterioration of metabolism responsible for early involution of the thymus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00334006
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