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  • 1990-1994  (325)
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  • 1
    Digitale Medien
    Digitale Medien
    No direct correlation between HLA-DPB1 and antibodies against recombinant Ro (SS-A)/La (SS-B) proteins in systemic lupus erythematosus (1993)
    Springer
    Rheumatology international 13 (1993), S. 155-158 
    Details
    ISSN: 1437-160X
    Schlagwort(e): Systemic lupus erythematosus ; HLA-DP ; Ro (SS-A) autoantibodies ; La (SS-B) autoantibodies ; Genetics
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary We investigated the association of HLA-DPB1 alleles with the occurrence of autoantibodies against Ro (SS-A) or La (SS-B) using recombinant 52kD-Ro, 60 kD-Ro and La proteins in 177 German patients with systemic lupus erythematosus (SLE). A significant increase in the frequency of DPB1 *0101 is observed in SLE patients compared to healthy controls (P corr.〈0.004). Antibodies against 52 kD-Ro, 60 kD-Ro and La are tested by ELISA and are found with a frequency of 25.4%, 33.9% and 17.5% in the patients, respectively. An association with HLA-DPB1 *0101 is observed for antibodies against La (P〈0.01) and 52 kD-Ro (P〈0.01), but not for 60 kD-Ro in the absence of La/52 kD-Ro. Since there is a strong linkage disequilibrium between DPB1 *0101 and DR3 in the normal population and in SLE patients, and since there is an association between DR3 and SLE, as well as between DR3 and the occurrence of recombinant Ro/La antibodies in SLE patients, we investigated whether DPB1 *0101 is associated per se or via linkage disequilibrium with DR3. DPB1 *0101 in the absence of DR3 is not more common in patients than in controls and not in patients with autoantibodies to Ro and La than without antoantibodies. We conclude that there is no evidence for a direct involvement of DPB1 *0101 in the production of Ro/La autoantibodies in SLE patients.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00301263
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  • 2
    Digitale Medien
    Digitale Medien
    The clinical spectrum of Friedreich's ataxia in German families showing linkage to the FRDA locus on chromosome 9 (1993)
    Springer
    Journal of molecular medicine 71 (1993), S. 109-114 
    Details
    ISSN: 1432-1440
    Schlagwort(e): Hereditary ataxias ; Friedreich's ataxia ; Genetics ; FRDA locus ; Chromosome 9
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary The clinical features of Friedreich's ataxia are described and reevaluated in a group of 14 German patients from 9 independent families. In contrast to previous studies, demonstration of linkage to the Friedreich's ataxia locus (FRDA) on chromosome 9p allowed confirmation of the genetic homogeneity of the disease in the patients under study. Marked variability within families was observed for age of onset of the disease (4–24 years) and for age of becoming wheelchair bound (17–37 years). Electrocardiographic changes were present in all and echocardiographic changes in 50% of the patients. Pathological changes of visual evoked potentials were detected in only 50% of the patients while brainstem auditory evoked potentials and somatosensory evoked potentials were always abnormal.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00179990
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  • 3
    Digitale Medien
    Digitale Medien
    On the genetics of complex partial seizures: waking and sleep EEGs in siblings (1993)
    Springer
    Journal of neurology 240 (1993), S. 151-155 
    Details
    ISSN: 1432-1459
    Schlagwort(e): Genetics ; Complex partial seizures ; Waking and sleep EEGs ; Siblings
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract Waking and sleep EEGs were recorded in 29 siblings of 19 patients with complex partial seizures. At least 1 sibling with epileptic activity (EA) was found for 36.8% of the patients. Taking the 29 siblings as a basis, in 7 EA was recorded. Most EA was seen during sleep in stage C (29%). More EA was recorded in female siblings (28% :18%) and in siblings of female patients (56% :20%). All EA was seen in the age range 5–14 years. Siblings with occipital theta-delta activity with a generalization tendency showed more EA (59%) than those without this pattern (8%). Of the siblings of patients with generalized EA 50% showed EA, but only 25% of those of patients with localized EEG patterns.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00857520
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  • 4
    Digitale Medien
    Digitale Medien
    Rhabdoid tumours of the central nervous system (1993)
    Springer
    Virchows Archiv 422 (1993), S. 81-85 
    Details
    ISSN: 1432-2307
    Schlagwort(e): Rhabdoid tumour ; Central nervous system ; Immunocytochemistry ; Ultrastructure
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary Three cases of rhabdoid tumour of the central nervous system arising in a supratentorial location are reported. The patients were 18, 14, and 7 years old. All three tumours showed a common morphology. The neoplastic cells were usually globoid with round nuclei and prominent nucleoli and large acidophilic, cytoplasmic inclusions were present in many of them. These inclusions showed strong immunoreactivity for vimentin, weak immunoreactivity for epithelial membrane antigen and focal immunoreactivity for cytokeratins. Ultrastructurally they were made up of whorls of intermediate filaments, 8–10 nm in thickness. Rhabdoid tumours of the central nervous system, whatever the cell of origin, appear to be an independent entity with identifiable histology and aggressive behaviour.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01605137
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  • 5
    Digitale Medien
    Digitale Medien
    Localized amyloidosis in squamous cell carcinoma of uterine cervix: Electron microscopic features of nodular and star-like amyloid deposits (1993)
    Springer
    Virchows Archiv 422 (1993), S. 225-231 
    Details
    ISSN: 1432-2307
    Schlagwort(e): Amyloid ; Amyloidosis ; Squamous cell carcinoma ; Keratin ; Ultrastructure
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract An ultrastructural study of amyloid deposits in four cases of squamous cell carcinoma of uterine cervix was performed. The amyloid deposits reacted with anti-keratin antiserum on frozen sections. Amyloid deposits showed nodular (4 cases) and star-like forms (3 cases). Nodular amyloid deposits were composed of slightly whorled fibrils, measuring 7–10 nm in width. Some of them contained cellular debris and thicker, more electron-dense filaments than amyloid fibrils. In three cases, filamentous tumour cells and filamentous masses were observed together with amyloid. Star-like amyloid deposits were composed of bundles of straight amyloid fibrils. Some of the tumour cells in contact with star-like amyloid deposits had deep cytoplasmic invaginations, where closely packed amyloid fibrils were arrayed in parallel fasion. In addition, a few tumour cells had membrane-bound amyloid fibrils in the cytoplasm. It is suggested that nodular amyloid deposits are derived from the tumour cells through filamentous degeneration. Amyloid fibrils in star-like amyloid deposits are thought to be formed within the cytoplasm or in the vicinity of invaginated cytoplasmic membranes of the tumour cells.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01621806
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  • 6
    Digitale Medien
    Digitale Medien
    Widening of capillary neck and alteration of extracellular matrix ultrastructure in diabetic rat glomerulus as revealed by computer morphometry and improved tissue processing (1993)
    Springer
    Virchows Archiv 423 (1993), S. 121-129 
    Details
    ISSN: 1432-2307
    Schlagwort(e): Ultrastructure ; Morphometry ; Diabetic nephropathy ; Rat
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract Morphological and morphometric studies of glomeruli were carried out in streptozotocin-induced diabetic rats using improved tissue processing and computerized morphometry. Increased mesangial matrix, occupying the enlarged diabetic mesangium, contained an abundance of dark granular material in addition to the microfibrils which were usually found in the control glomeruli. In the diabetic glomeruli, the lamina densa was thick and heterogeneous showing a dense layer both on its epithelial and endothelial aspects, and the lamina rara externa contained more fibrils than in control rats. Detailed estimation of the absolute values of the various compartments of the diabetic glomeruli by using perfusion-flxed materials and a computer-assisted digitizer revealed that the volume and surface area of the mesangium were increased more extensively than those of the capillary; the enlargement of the mesangial-capillary interface area was the most pronounced among the morphometric changes of the diabetic glomeruli; and that the moderate increase in capillary volume was associated with an increased radius. Our quantitative results showed that capillaries in the diabetic glomeruli had an extensively wider neck which may be the first sign of structural damage to the glomerular tuft.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01606586
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  • 7
    Digitale Medien
    Digitale Medien
    Altered intestinal development after jejunal ligation in fetal sheep (1993)
    Springer
    Virchows Archiv 423 (1993), S. 45-50 
    Details
    ISSN: 1432-2307
    Schlagwort(e): Fetus ; Small intestine ; Ultrastructure ; Intestinal atresia
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract Experimental obstruction of the fetal small intestine resulted in massive hypertrophy of the segment proximal to the site of obstruction. Villus morphology was grossly abnormal. Enterocytes developed many irregular features, most notably cytoplasmic extensions (pseudopods, or blebs) from their apical surface. Distal to the site of obstruction, morphological anomalies which resembled those seen after experimental oesophageal ligation were found. These included delayed disappearance of the apical endocytic network, disrupted or absent microvilli, glycogen accumulation and inappropriate cell extrusion. Proximal to the obstruction, where stasis of swallowed fluid occurs, distension and abnormal intestinal development ensues. Distal to the obstruction where the intestine develops in the absence of swallowed fluid, development is also abnormal. The anomalies resemble those noted after oesophageal ligation in utero, and possibly are the results of reduced cellular nutrition. These results suggest that fetal ingestion provides the developing gastrointestinal tract with an important stimulus for normal growth.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01606431
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  • 8
    Digitale Medien
    Digitale Medien
    Comparative study of histopathological alterations during intestinal infection of mice with pathogenic and non-pathogenic strains ofYersinia enterocolitica serotype O:8 (1993)
    Springer
    Virchows Archiv 423 (1993), S. 97-103 
    Details
    ISSN: 1432-2307
    Schlagwort(e): Yersinia enterocolitica ; Peyer's patches ; Ultrastructure ; M-cell ; Invasiveness
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract Yersinia enterocolitica is an invasive pathogen capable of causing a wide spectrum of gastrointestinal diseases in man. While there is a considerable body of data on the invasiveness ofY. enterocolitica in vitro, little is known about the events in vivo leading to the translocation of the bacteria from the intestinal lumen into the ileal tissue. There is no detailed ultrastructural information describing the course of infection of pathogenicY. enterocolitica in comparison with an avirulent strain. We compared a virulent plasmid-bearing strain and an isogenic avirulent plasmid-free derivative strain ofY. enterocolitica serotype O∶8 at the ultrastructural level, in the established model of murine yersiniosis. At 12 h postinoculation we found no indications of an active invasion of the intestinal epithelium, although microcolonies of the pathogenic strain were detectable closely under the follicle-associated epithelium of the Peyer's patches. The plasmid-bearing strain ofY. enterocolitica affected the gut-associated lymphoid tissue which was destroyed 36 h post-infection. Unlike the pathogenic strain ofY. enterocolitica, the nonpathogenic plasmid-free strain caused no detectable morphological alterations in the ileal tissue by this time. Morphological evidence is provided thatYersinia does not invade the ileal epithelium in an active manner, as has been observed in vitro, but appears to be transported across the epithelial barrier by M-cells.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01606583
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  • 9
    Digitale Medien
    Digitale Medien
    Cytogenetic study of botryoid rhabdomyosarcoma of the uterine cervix (1993)
    Springer
    Virchows Archiv 422 (1993), S. 87-91 
    Details
    ISSN: 1432-2307
    Schlagwort(e): Botryoid rhabdomyosarcoma ; Uterine cervix ; Immunohistochemistry ; Ultrastructure ; Chromosomal analysis
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary We report a case of sarcoma botryoides of the uterine cervix occurring in a 19-year-old woman. By light microscopy the tumor showed round and spindle cells with hyperchromatic nuclei and, focally, a cambium layer subjacent to the surface epithelium and surrounding endocervical glands. Strap-shaped cells with and without cross-striations and small foci of immature cartilage were also present. Immunohistochemical studies showed positive staining within the tumor cells for myoglobin, desmin, vimentin, muscle-specific actin and CD56. By electron microscopy, tumor cells showed cytoplasmic filaments in an alternating pattern of thick and thin filaments. Chromosomal analysis demonstrated deletion of the short arm of chromosome 1, and trisomies 13 and 18. To our knowledge, this is the first reported case of sarcoma botryoides of the endocervix with chromosomal analysis.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01605138
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  • 10
    Digitale Medien
    Digitale Medien
    Cellular and matrix changes in drug abuser liver sinusoids: A semiquantitative and morphometric ultrastructural study (1993)
    Springer
    Virchows Archiv 422 (1993), S. 145-152 
    Details
    ISSN: 1432-2307
    Schlagwort(e): Heroin ; Sinusoids ; Fibrosis ; Ultrastructure ; Morphometry
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract The aim of the present work was to analyse, at the ultrastructural level, the action of heroin of 150 centrilobular sinusoids from liver biopsies of five intravenous drug abusers, who presented clinical and biological manifestations of hepatic impairment. A comparative study of 90 sinusoids from liver biopsies of three control patients was performed. Electron microscopic observations showed a thickening of the sinusoidal wall related to endothelial cell hypertrophy and to fibrosis of the space of Disse. This was generally associated with basement-membrane-like material and hepatocyte microvilli flattening. In addicts, hepatic vascular pole changes were a constant finding, accompanied by interhepatocyte space disjunction and perisinusoidal collagenization. Morphometric assessment confirmed a significant increase of sinusoidal wall surface, endothelial cell body and processes and Ito cell process surface was significantly different between the patient groups. This cellular hypertrophy may represent hyperactivation of the sinusoidal cell functional capacity, triggering the fibrogenesis in the space of Disse. While this mechanical barrier might hinder the free exchange through the space of Disse, it may equally well protect the liver against heroin toxicity.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01607166
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  • 11
    Digitale Medien
    Digitale Medien
    Ultrastructural study ofCryptococcus neoformans by quick-freezing and deep-etching method (1993)
    Springer
    Mycopathologia 121 (1993), S. 133-141 
    Details
    ISSN: 1573-0832
    Schlagwort(e): Capsule ; Cryptococcus neoformans ; Deep-etching ; Quick-freezing ; Ultrastructure
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Abstract The three-dimensional ultrastructure ofCryptococcus neoformans was studied by quick-freezing and deep-etching (QF-DE) method.C. neoformans, strain CDC551, was cultured on agar. The viable yeast cells (107 cells) were inoculated into each mouse from the tail vein. Three weeks after the inoculation, the brains of the mice were perfused with fixatives, quickly frozen, freeze-fractured, deeply etched and rotary shadowed with platinum and carbon. In addition, the viable cells ofC. neoformans on agar were picked up and quickly frozen, and replica membranes were prepared as described above. The ultrastructure ofC. neoformans was three-dimensionally demonstrated by the QF-DE method. The capsule was composed of fine meshworks of microfibrils (10–13 nm in diameter), which were directly attached to the cell walls. The capsule of the in vivo yeasts (yeast cells in the brain lesion) was thicker than that of the in vitro yeasts (yeast cells on agar culture). At the outer part of the cell wall, a particle-accumulating layer was observed. This layer in vivo was thicker than that in vitro. Occasionally, the yeast cells were ingested by phagocytes in the mouse brain. Although the cytoplasm of such yeast cells was destroyed, the capsular meshworks were well preserved. The ultrastructure of the capsule was the same both in cultured and phagocytized yeasts in the cystic lesions of the brains. This lack of morphological changes of the capsular meshworks suggests that they are resistant to the digestion by phagocytes. This stability of capsular structures may provide one of the important pathogenic factors in cystic lesions byC. neoformans.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01104068
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  • 12
    Digitale Medien
    Digitale Medien
    Morphological assessment of ethyl choline mustard aziridinium-induced neurotoxicity in rat brain reaggregate cultures (1993)
    Springer
    Acta neuropathologica 86 (1993), S. 154-162 
    Details
    ISSN: 1432-0533
    Schlagwort(e): Ethylcholine mustard aziridinium (ECMA, AF64A) ; Brain reaggregate cultures ; Ultrastructure
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary Foetal rat brain reaggregate cultures have been employed to investigate the morphological changes associated with the neurotoxic action of ethylcholine mustard aziridinium (ECMA). In a companion study we provided evidence for apparent selective cholinergic neurotoxicity. Exposure of 9-day-old cultures to 12.5 μM ECMA for 3 days produced dilatation of selected axon preterminals and terminals in the outer core tissue layer. Axoplasm in these dilated terminals was electron lucent and contained a flocculent, plasma-like material with remnants of the smooth endoplasmic reticulum. Their synaptic vesicle content was much reduced or, absent. Microglial cells were engaged in phagocytosis of these effete structures and a few necrotic neurons were enveloped by glial processes. Exposure to 50 μM ECMA produced widespread necrosis with some surviving neurons, surrounded by the still-persisting capsular layer. Treatment with 100 μM ECMA generated a greater extent of tissue necrosis, with only a few surviving neurons and glial cells being contained within the necrotic tissue mass. Reaggregates frequently disintegrated following capsule loss. Our results indicate that the initial morphological manifestation of ECMA-induced toxicity is dilatation of axon terminals, that are probably of cholinergic origin and are targeted due to their possession of the high-affinity choline transport system which is unique to these neurons.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00334882
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  • 13
    Digitale Medien
    Digitale Medien
    Nephrosialidosis: ultrastructural and lectin histochemical study (1993)
    Springer
    Acta neuropathologica 86 (1993), S. 198-205 
    Details
    ISSN: 1432-0533
    Schlagwort(e): Nephrosialidosis ; Sialidosis ; α-neuraminidase deficiency ; Ultrastructure ; Lectin histochemistry
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary The neuropathological findings in a Japanese male with nephrosialidosis are reported. Clinically, coarse face, psychomotor retardation, macular cherryred spot and proteinuria were noted at 1 year and 7 months. He was diagnosed to have nephrosialidosis on the basis of a deficiency of α-neuraminidase activity in both lymphocytes and cultured skin fibroblasts, and of severe glomerular and tubular involvement on renal biopsy. He died of multiple organ failure at 8 years and 6 months. There were numerous vacuoles and storage materials in visceral organs, particularly in the glomerular and tubular epithelial cells of the kidney and Kupffer cells as well as hepatocytes in the liver. Neuropathological examination revealed severe neuronal storage in the selected part of the central nervous system; lower motor neurons of the brain stem and spinal anterior horn cells, as well as neurons in the basal nucleus of Meynert. In the peripheral nervous system, sympathetic ganglia were severely affected. There was little or no neuronal storage in the basal ganglia, cerebral cortex or cerebellum, and demyelination was not found. Electron microscopic examination showed fine wavy multilamellar structures in the spinal anterior horn cells or Zebra body-like structures in the neurons of the Meynert's basal nucleus. Lectin histochemistry was positive for wheat germ agglutinin, Ricinus communis agglutinin-1 and peanut agglutinin within distended neurons. We conclude that the neuropathological feature in nephrosialidosis is not specific except for the selectiveness of the anatomical sites of involvement. It shares some aspects found in other types of sialidosis or galactosialidosis.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00334891
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  • 14
    Digitale Medien
    Digitale Medien
    A simple method of rapid freezing adequately preserves brain tissue for immunocytochemistry, light and electron microscopic examination (1993)
    Springer
    Acta neuropathologica 86 (1993), S. 645-650 
    Details
    ISSN: 1432-0533
    Schlagwort(e): Brain ; Freezing ; Immunocytochemistry ; Microscopy ; Ultrastructure
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract A simple and reproducible method for cryo-preservation of brain tissue from patients with Alzheimer's disease is described. Fresh brain slices (1 cm thick) obtained less than 6 h postmortem are placed in sealed plastic bags, sandwiched between 0.3-cm-thick aluminium sheets, and frozen by placing the entire “sandwich” between layers of dry ice pellets. The frozen brain slices are stored at −85 °C. Specific anatomic areas can be retrieved at any time for light and electron microscopic, immunocytochemical, autoradiographic and neurochemical studies.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00294305
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  • 15
    Digitale Medien
    Digitale Medien
    Stereological and morphometric analysis of dermal fibroblasts before and after bone marrow transplantation in a case of mucopolysaccharidosis I Scheie phenotype (1993)
    Springer
    Acta neuropathologica 86 (1993), S. 21-28 
    Details
    ISSN: 1432-0533
    Schlagwort(e): Mucopolysaccharidosis I Scheie phenotype ; Bone marrow transplantation ; Fibroblasts ; Stereologic analysis ; Ultrastructure
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary Bone marrow transplantation (BMT) has been used therapeutically in several types of mucopoly-saccharidoses (MPS) and other inherited metabolic disorders. Fibroblasts are severely affected in MPS due to the intralysosomal accumulation of glycosaminoglycans. We report a stereological and morphometric study at light and electron microscopy levels of dermal fibroblasts before and 21 months after BMT in a young girl with MPS I Scheie phenotype (MPS I-S). Dermal fibroblasts showed remarkable morphological changes although their density, expressed as number of fibroblasts per unit volume of dermis (number density), was not modified in the post-BMT samples as compared to pre-BMT ones. Stereological and morphometric parameters referring to cell characteristics of post-BMT fibroblasts (nuclear and cell surface densities, and both nucleus/cell and cell/nucleus volume densities) showed significant differences when compared with pre-BMT fibroblasts, and non-significant differences regarding control cells. On the other hand, quantitative parameters of the lysosomal compartment from post-BMT fibroblasts showed intermediate values between pre-BMT and control fibroblasts. These results, at cellular level, are in agreement with previous biochemical and clinical results, and clearly showed a progressive course to a non-pathological state. All parameters estimated may be considered useful tools in evaluating the success of BMT. These parameters provide quantitative data which can be statistically compared, showing the changes due to the reduction of storage material after BMT. Cell/nucleus volume density is especially interesting since not only is it easy to estimate, even by automatic procedures, but it could also constitute a numerical expression of skin anatomopathological analyses performed post-BMT.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00454894
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  • 16
    Digitale Medien
    Digitale Medien
    Rosenthal fibers producing a granular cell appearance in a glioblastoma (1993)
    Springer
    Acta neuropathologica 86 (1993), S. 100-104 
    Details
    ISSN: 1432-0533
    Schlagwort(e): Glioblastoma ; Granular cell tumor ; Intermediate filaments ; Rosenthal fibers ; Ultrastructure
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary In this report we describe a glioblastoma multiforme with focal granular cell change. In most astroglial tumors with granular cells, the granular appearance is due to the presence of periodic acid-Schiff-positive, membrane-bound cellular debris. In the present case the granular appearance was due to the presence of many small Rosenthal fibers, which were immunoreactive for glial fibrillary acidic protein, vimentin, ubiquitin, and heat-shock protein 27, but not for α-B crystallin. The ultrastructural characteristics are described. These findings demonstrate that granulofilamentous inclusions with the appearance of Rosenthal fibers in glial tumors are a structurally heterogeneous feature.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00454907
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  • 17
    Digitale Medien
    Digitale Medien
    Intracranial extraskeletal myxoid chondrosarcoma with special reference to lamellar inclusions in the rough endoplasmic reticulum (1993)
    Springer
    Acta neuropathologica 86 (1993), S. 525-528 
    Details
    ISSN: 1432-0533
    Schlagwort(e): Extraskeretal myxoid chondrosarcoma ; Ultrastructure ; Collagen type II ; Rough endoplasmic reticulum ; Lamellar inclusion
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract An extraskeletal myxoid chondrosarcoma in the pineal region was studied by light and electron microscopy. The immunohistochemical positivity for S-100 protein, vimentin and collagen type II favored a chondrocytic origin of the tumor. In addition to the well-described ultrastructural features suggestive of a cartilagenous nature, this tumor had unusual lamellar inclusions in the rough endoplasmic reticulum. To the best of our knowledge, this is the first report of these special inclusion bodies in a myxoid chondrosarcoma.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00228591
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  • 18
    Digitale Medien
    Digitale Medien
    Uncommon types of polyglucosan bodies in the human brain: distribution and relation to disease (1993)
    Springer
    Acta neuropathologica 86 (1993), S. 484-490 
    Details
    ISSN: 1432-0533
    Schlagwort(e): Polyglucosan bodies ; Bielschowsky bodies ; Adult polyglucosan body disease ; Immunohistochemistry ; Ultrastructure
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract The significance of the development of polyglucosan bodies (PBs) in the CNS is incompletely understood. We present the clinicopathological features of three autopsy cases with numerous PBs other than the common corpora amylacea or Lafora bodies. The first patient had pleomorphic PBs in the neuronal processes of pallidum and substantia nigra which, thus, are consistent with Bielschowsky bodies. Bielschowsky bodies involved also the hypothalamus and tegmentum of midbrain and medulla. The present case was the first not associated with any clinical symptoms. The second patient also had incidental Bielschowsky bodies in the external pallidum, substantia nigra, and pallidothalamic, pallidonigral and nigrostriatal tracts. Additionally, unique clusters of small PBs appeared in the cerebral cortex, putamen, pallidum, and caudate nucleus. Immunostaining suggested that these small clustered PBs were located in the cytoplasm and processes of astrocytes. Ultrastructurally, these clustered PBs were in agreement with previous descriptions of PBs. The third patient had adult polyglucosan body disease. Most PBs in the white matter were corpora amylacea situated in astrocytic processes or axons. In the gray matter, many pleomorphic PBs resembling Bielschowsky bodies occurred in neuronal processes. In the peripheral nervous system, a few PBs were seen in myelinated axons. The following conclusions may be drawn from this study: (1) each type of PBs develops in distinct cell types of the human brain in variable distribution; (2) Bielschowsky bodies may not manifest clinically; (3) PBs other than corpora amylacea or Lafora bodies may be distributed in localized or diffuse patterns; (4) in the localized pattern (patients 1 and 2), PBs occur as Bielschowsky bodies or clustered PBs, and tend to involve certain systems (pallidum, striatum, and substantia nigra); and (5) in the diffuse pattern (patient 3), PBs develop as corpora amylacea and Bielschowsky-like bodies of adult polyglucosan body disease.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00228584
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  • 19
    Digitale Medien
    Digitale Medien
    Ontogenesis of the rabbit pituitary intermediate lobe (1993)
    Springer
    Anatomy and embryology 187 (1993), S. 87-97 
    Details
    ISSN: 1432-0568
    Schlagwort(e): Pituitary intermediate lobe ; Rabbit ; Ontogenesis ; Immunocytochemistry ; Ultrastructure
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary The development of the intermediate lobe of the rabbit pituitary was investigated by light and electron microscopy and by using immunocytological techniques. The first immunoreactive melanotrophic cells were detected at the fetal day 17 in the dorsal zone of Rathke's pouch epithelium facing the neural lobe; this coincided ultrastructurally with the appearance in this area of a few cells exhibiting secretory vesicles and granular condensations in the Golgi saccules. The differentiation of the gland probably required an infundibular inductive effect. Secretory cells increased in number following a dorsoventral gradient during the next fetal and neonatal stages until postnatal day 20, the stage at which the intermediate lobe exhibited its definitive organization. The gland innervation occurred during the first days after birth. The advent of these oxytocin- and neurophysin-immunoreactive fibres coincided with an obvious stimulation of the synthetic activity of the melanotrophic cells. The possible neurotrophic effect of these cells on their innervating system remains to be established.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00208199
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  • 20
    Digitale Medien
    Digitale Medien
    The ciliary ganglion of the cat: a light and electron microscopic study (1993)
    Springer
    Anatomy and embryology 187 (1993), S. 591-599 
    Details
    ISSN: 1432-0568
    Schlagwort(e): Ciliary ganglion ; Quantitative study ; Ultrastructure ; Cat
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract The ciliary ganglia of eight healthy adult cats were studied by light and electron microscopy. The ganglion, measuring about 2 mm in length, was consistently found to be attached to the branch from the oculomotor nerve supplying the inferior oblique muscle. The number of neurons varied from 2773 to 3794 after applying Abercrombie's correction. The mean of average somal diameter of the neurons was 36.5 μm (SD = 5.0 μm) and the mean of somal cross-sectional area was 904.2 μm2 (SD = 262.8 μm2). The mean of average nuclear diameter was 13.9 urn (SD = 1.8 μm) and the mean of nuclear cross-sectional area was 142.2 μm2 (SD = 37.1 μm2). The mean of the aspect ratios of the soma and nucleus were 1.2 (SD = 0.1) and 1.1 (SD = 0.1) respectively. The frequency distributions of these parameters were all unimodal. Under the light microscope, the Nissl granules in the neurons were prominent and were distributed peripherally, perinuclearly or randomly in the cytoplasm. Under the electron microscope, the rough endoplasmic reticulum showed a similar pattern of distribution in the cytoplasm. In some neurons, glycogen-like granules were present; these were either distributed randomly throughout the cell, or aligned in single rows in relation to sub-surface cisterns and between the cisterns of smooth and rough endoplasmic reticulum. Most of the dendrites were short protrusions from the cell body; some contained glycogen-like granules. Occasionally, the dendritic protrusions were electron-dense. All the synapses encountered were axodendritic. In most axon terminals, the synaptic vesicles were spherical and measured 30–50 nm in diameter; in some, they were flattened, measuring 50 nm by 20 nm. Some axon terminals containing either spherical or flattened synaptic vesicles also contained large dense-cored vesicles that measured 80–100 nm, while their dense core measured 40–60 nm.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00214438
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  • 21
    Digitale Medien
    Digitale Medien
    Morphological analysis of in vitro human hair growth (1993)
    Springer
    Archives of dermatological research 285 (1993), S. 158-164 
    Details
    ISSN: 1432-069X
    Schlagwort(e): Hair follicle ; Culture ; Ultrastructure
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract The histological and ultrastructural aspect of normal human hair follicles maintained ex vivo for 12 days was evaluated. Anagen hair follicles, dissected free of contaminating connective tissue, were maintained for up to 12 days in a serum-free medium. Macroscopic observations revealed continued viability for 12 days, at which time some follicles involuted in a manner morphologically similar to catagen. Increased growth of maintained follicles was measured from the abrupt ending of the connective tissue sheath (CTS), as no increase in this component was observed from initiation of culture. In general follicles maintained up to 8 days exhibited little divergence from normal in vivo morphologies including the persistence of functional hair bulb melanocytes — a marker of anagen. After this time melanin granules were present in dermal papilla cells, as occurs during impending involution in vivo. Heterotypic cell contact occurred in the middle to upper follicle between outer root sheath (ORS) keratinocytes and disorganized CTS. Herniation of some ORS cells away from the follicle and the occurrence of loose desmosomal junctions between ORS keratinocytes reflected loss of normal follicular cell interactions in upper follicles maintained after 8 days. Continued follicle growth correlated with the presence of mitotic matrix keratinocytes even at 12 days. After 12 days in culture most follicles involuted displaying apoptotic-like keratinocytes and hair bulb melanocytes and the presence of highly keratinized hair ‘club’ structures. While most follicles exhibited this orderly sequence of events, a few follicles involuted after 24 h with synchronous degeneration of all cells. Two follicles exhibited upregulated cortical cell differentiation at the level of the dermal papilla (DP). Normal cell cytoplasmic constituents were replaced with ribosomes and keratin bundles. This study reveals for the first time the histology and ultrastructure of normal hair follicles in culture for up to 12 days in serum-free medium. Although involuted hair follicles may exhibit some features of catagen, it is possible that the mechanisms involved are entirely different.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01112919
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  • 22
    Digitale Medien
    Digitale Medien
    Human hexokinase II: localization of the polymorphic gene to chromosome 2 (1993)
    Springer
    Diabetologia 36 (1993), S. 1299-1302 
    Details
    ISSN: 1432-0428
    Schlagwort(e): Genetics ; DNA polymorphism ; glucose ; phosphorylation ; glycolysis ; chromosome 2 ; insulin resistance
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary Type 2 (non-insulin-dependent) diabetes mellitus is characterized by decreased levels of glucose 6-phosphate in skeletal muscle. It has been suggested that the lower concentrations of glucose 6-phosphate contribute to the defect in glucose metabolism noted in muscle tissue of subjects with Type 2 diabetes or subjects at increased risk of developing Type 2 diabetes. Lower levels of glucose 6-phosphate could be due to a defect in glucose uptake, or phosphorylation, or both. Hexokinase II is the isozyme of hexokinase that is expressed in skeletal muscle and is responsible for catalysing the phosphorylation of glucose in this tissue. The recent demonstration that mutations in another member of this family of glucose phosphorylating enzymes, glucokinase, can lead to the development of Type 2 diabetes prompted us to begin to examine the possible role of hexokinase II in the development of this genetically heterogeneous disorder. As a first step, we have cloned the human hexokinase II gene (HK2) and mapped it to human chromosome 2, band p13.1, by fluorescence in situ hybridization to metaphase chromosomes. In addition, we have identified and characterized a simple tandem repeat DNA polymorphism in HK2 and used this DNA polymorphism to localize this gene within the genetic linkage map of chromosome 2.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00400809
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  • 23
    Digitale Medien
    Digitale Medien
    HLA-associated susceptibility to Type 2 (non-insulin-dependent) diabetes mellitus: the Wadena City Health Study (1993)
    Springer
    Diabetologia 36 (1993), S. 234-238 
    Details
    ISSN: 1432-0428
    Schlagwort(e): Genetics ; Type 2 (non-insulin-dependent) diabetes mellitus ; HLA
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary Epidemiologic data suggest that a parental history of Type 2 (non-insulin-dependent) diabetes mellitus increases the risk of Type 1 (insulin-dependent) diabetes in siblings of a Type 1 diabetes proband. This increase in risk is consistent with a shared genetic susceptibility between Type 1 and Type 2 diabetes. We have previously reported evidence that HLA-DR4-linked factors may represent a homogeneous subset of diabetes susceptibility. First, HLA-DR4 frequency was higher in Type 1 diabetic study subjects with a Type 2 diabetic parent than in Type 1 diabetic subjects whose parents were not diabetic. Second, a DR4-haplotype was transmitted from the Type 2 diabetic parent to the Type 1 offspring more often than expected. These data are consistent with the hypothesis that families with a Type 2 diabetic parent and Type 1 diabetic child, heavily determined by HLA-DR4 linked factors, may represent a homogeneous subset of diabetes susceptibility. In this report, we further explore the relationship between the high-risk HLA antigen (HLA-DR4) in study subjects with differing glycaemic status (National Diabetes Data Group criteria). In this community-based study, we find evidence that HLA-DR4 is increased in study subjects with Type 2 diabetes and may be a marker for Type 2 diabetes susceptibility.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00399956
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  • 24
    Digitale Medien
    Digitale Medien
    Mayflies (ephemeroptera), the most “primitive” winged insects, have telotrophic meroistic ovaries (1993)
    Springer
    Development genes and evolution 203 (1993), S. 18-27 
    Details
    ISSN: 1432-041X
    Schlagwort(e): Oogenesis ; Germ line cell cluster ; Oocyte determination ; Ultrastructure ; Mayflies
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Germ line cell cluster formation in ovarioles of three different stages, each from a different mayfly species, was studied using ultra-thin serial sectioning. In the analysed ovariole of Cloeön sp., only one linear, zigzag germ line cell cluster was found, consisting of sibling cells connected by intercellular bridges which represent remnants of preceding synchronized mitotic cycles followed by incomplete cytokinesis. A polyfusome stretched through all sibling cells. At the tip of the ovariole, cytokinesis occurred without preceding division of nuclei; thus, intercellular bridges were lined up but the remaining cytoplasm between the bridges had no nuclei. The analysed Siphlonurus armatus vitellarium contained five oocytes at different stages of development. Each oocyte in the vitellarium was connected via a nutritive cord to the linear cluster of its sibling cells in the terminal trophic chamber. Each cluster had the same architecture as was found in Cloëon. The 3-dimensional arrangement and distribution of closed intercellular bridges strongly suggest that all five clusters are derived from a single primary clone. The position of oocytes within each cluster is random. However, each oocyte is embraced by follicular or prefollicular cells whilst all other sibling cells are enclosed by somatic inner sheath cells, clearly distinguishable from prefollicular cells. In the analysed ovariole of Ephemerella ignita, two small linear clusters were found in the tropharium beside two single cells, two isolated cytoplasmic bags with intercellular bridges but no nuclei, and some degenerating aggregates. One cluster was still connected to a growing oocyte via a nutritive cord. In all species the nurse cells remained small and no indications of polyploidization were found. We suggest that this ancient and previously unknown telotrophic meroistic ovary has evolved directly from panoistic ancestors.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00539886
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  • 25
    Digitale Medien
    Digitale Medien
    Ultrastructural expression of cytoplasmic male sterility in sugar beet (Beta vulgaris L.) (1993)
    Springer
    Sexual plant reproduction 6 (1993), S. 22-32 
    Details
    ISSN: 1432-2145
    Schlagwort(e): Cytoplasmic male sterility ; Ultrastructure ; Mitochondria morphometry ; Beta vulgaris L
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The development of microspore mother cells (MMC) and tapetum in male-fertile and male-sterile anthers of Beta vulgaris L. was compared at the electron microscope level. These studies were complemented by morphometric analyses of mitochondria in both tissues through successive stages of microsporogenesis. The earliest irregularities in the ultrastructure of male-sterile anthers were noted within the tapetum at the tetrad stage. These disturbances were initially expressed by a slight reduction in mitochondrial size and the appearance of concentric configurations of endoplasmic reticulum. As development proceeded, a further decrease in mitochondrial size become more conspicuous and was accompanied by a reduction in ribosome population and a failure of the tapetum to produce Ubisch bodies. This failure to produce Ubisch bodies is reflected in the underdevelopment of sterile microspore exine.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00227579
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  • 26
    Digitale Medien
    Digitale Medien
    Megaspore development in Selaginella. I. “Wicks”, their presence, ultrastructure, and presumed function (1993)
    Springer
    Sexual plant reproduction 6 (1993), S. 98-107 
    Details
    ISSN: 1432-2145
    Schlagwort(e): Selaginella ; Megaspore ; Exospore ; Ultrastructure ; Tapetal cells ; Plasmodesmata
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Structures have been found in the locular space between the tapetal cells and megaspores in Selaginella argentea and S. kraussiana that enter the megaspore wall and extend to the plasma membrane of the megaspore cytoplasm. We have called these structures “wicks”. Unless special fixation procedures are used wicks are either very poorly preserved or not apparent. Wicks appear to be routes for the transport of materials from the tapetum to developing megaspores. The entry of the wicks into the megaspore wall and their passage throughout the wall implies that the megaspore wall of Selaginella is a three-dimensional mesh-work of inter-connecting spaces. Wicks have several macromolecular-sized subunits, and the results of our histochemical reactions indicated the presence of glycoprotein and/or mucopolysaccharide. X-ray microanalysis of the S. convoluta exospore showed that silicon is present in rod-shaped structures between units of the exospore in mature megaspores. Because of the size and form of the structures between the exospore units we consider that they are remnants of wicks stabilized by silicon.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00227654
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  • 27
    Digitale Medien
    Digitale Medien
    Chemical agents that inhibit pollen development: effects of the phenyl-cinnoline carboxylates SC-1058 and SC-1271 on the ultrastructure of developing wheat anthers (Triticum aestivum L. var Yecora rojò) (1993)
    Springer
    Sexual plant reproduction 6 (1993), S. 108-212 
    Details
    ISSN: 1432-2145
    Schlagwort(e): Wheat pollen ; Chemical hybridizing agents ; Male sterility ; Ultrastructure
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Phenylcinnoline carboxylate compounds SC-1058 and SC-1271 cause complete male sterility in wheat when applied at suitable dosages at the pre-meiotic stage of anther development. Anthers from treated and untreated plants were compared using light and electron microscopy from the pre-meiotic stage through the formation of nearly mature pollen. Overall anther development is gradually slowed in treated plants and pollen development is generally arrested in the late prevacuolate or early vacuolate microspore stage, although the first pollen mitosis does sometimes occur. The sporopollenin-containing exine walls are thinner, and show abnormally developed foot and tectum layers with sparse connecting baculi. Microspore cytoplasm degenerates and the cells eventually collapse. At the early, prevacuolate, free microspore stage treated tapetal cells hypertrophy, expanding into the locule. They contain abnormally large vacuoles that appear to form from the fusion of secretory vesicles, and some vacuoles contain electrondense deposits. The sporopollenin-containing orbicular wall and Ubisch bodies are retarded in their development and are structurally deformed. Acetolysis of whole anthers and of thick sections shows that the sporopollen-in-containing structures of treated materials are greatly reduced in thickness and are less rigid than in the control. We conclude that application of these compounds causes interference with the secretory function of tapetal cells which supplies sporopollenin cell-wall polymers to the exine of the microspores and to the tapetal orbicular wall and associated Ubisch bodies. Interference with the tapetal secretion of other nutrients required for microspore development is strongly suggested.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00227655
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  • 28
    Digitale Medien
    Digitale Medien
    The embellum: a newly defined structure in the grass ovule (1993)
    Springer
    Sexual plant reproduction 6 (1993), S. 191-198 
    Details
    ISSN: 1432-2145
    Schlagwort(e): Micropyle ; Transfer cells ; Ultrastructure ; Nucellus ; Poaceae
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Several kinds of outgrowth from the grass ovule are known. Attention is focused here on one outgrowth that occurs within or around the micropyle and is of nucellar origin. Grass species in which it is currently known to occur are listed and examples of variants briefly described. Attention is concentrated upon Pennisetum, where the cell structure is described in detail with a series of electron photomicrographs. The tissue representing an aggregation of these transfer cells is newly named with the term ‘embellum’, and its significance for pollen tube growth is considered.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00228648
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  • 29
    Digitale Medien
    Digitale Medien
    Ultrastructural changes in the appendix of the Sauromatum guttatum inflorescence during anthesis (1993)
    Springer
    Sexual plant reproduction 6 (1993), S. 153-170 
    Details
    ISSN: 1432-2145
    Schlagwort(e): Appendix ; Sauromatum guttatum ; Ultrastructure ; Mitochondrion ; Amyloplast ; Peroxisome
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The ultrastructure of the epidermal and sub-epidermal cells of the appendix of the Sauromatum guttatum inflorescence reveals developmental changes during anthesis. These changes precede, and probably make possible, heat and odor production. Two days before D-day (the day of heat production and inflorescence-opening) the mitochondria of the epidermis divide; apparent division of the amyloplasts was observed at the same time. The presence of lipid bodies and peroxisomes in the epidermis was clearly evident. On D-day, the epidermis becomes a continuous layer in which the cell walls separating two adjacent cells disappear. At the same time, in the sub-epidermal cells, the mitochondria and the amyloplasts undergo division. The mitochondria become electron-dense, and their DNA is clearly visible. On that day, lipids as well as starch are being depleted. The peroxisomes change in structure every day, from D-2 to D-day. It has also been demonstrated by histochemical techniques that during anthesis the activity of cytochrome c oxidase (3,3-diaminobenzidine as a substrate) decreases whereas the activity of NADH dehydrogenase [tetrazolium salts: nitro-blue tetrazolium chloride (NBT) or neotetrazolium chloride (NT) in the presence of NADH], increases. Oxygen consumption of isolated mitochondria from the D-day appendix was inhibited in the presence of the two tetrazolium salts to a different degree: oxidation of NADH in the presence of NBT was the most sensitive to inhibition, more so than the oxidation of malate and succinate. NT was less effective as an inhibitor in the presence of those three respiratory substrates.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00228644
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  • 30
    Digitale Medien
    Digitale Medien
    Hirschsprung disease: Paternal transmission to a son (1993)
    Springer
    European journal of pediatrics 152 (1993), S. 467-468 
    Details
    ISSN: 1432-1076
    Schlagwort(e): Hirschsprung disease ; Familial occurrence ; Genetics
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract Hirschsprung disease (HD) is genetically heterogeneous with approximately 4% familial occurrence. The recurrence risk is higher in patients with severe involvement. We describe the transmission of histotopochemically proven HD from a father with long aganglionic segment disease to a son with ultrashort segment disease. This observation suggests that the length of involvement in HD is related to the variable expression of the gene defect. It also suggests autosomal dominant inheritance of HD.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01955050
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  • 31
    Digitale Medien
    Digitale Medien
    Ultrastructure of the cortical epithelium of the rat thymus after in vivo exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) (1993)
    Springer
    Archives of toxicology 67 (1993), S. 558-564 
    Details
    ISSN: 1432-0738
    Schlagwort(e): 2,3,7,8-Tetrachlorodibenzo-p-dioxin ; TCDD ; Thymus ; Epithelium ; Ultrastructure ; Rat
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) is known for inducing cortical atrophy in the rat thymus. The present study was conducted to provide ultrastructural evidence for the cortical epithelium to be a target for TCDD in vivo. Juvenile male Wistar rats were orally intubated once with either 50 or 150 μg/kg TCDD and killed 4 or 10 days thereafter. Major changes were found in the cortical thymic epithelium. First, a relative shift occurred from “pale” to darker cortical epithelial cell types, as judged by their nuclear and cytoplasmic electron density. This effect was most prominent at 10 days after exposure to 150 μg/kg TCDD. The increased electron density of the cortical epithelium indicates an altered state of cellular differentiation. Secondly, at the 150 μg/kg dose level focal epithelial cell aggregates were seen both at day 4 and day 10 after administration. This aggregation may either be compound induced or represent a secondary event to the collapse of the thymic stroma. Thirdly, increased vacuolation of cortical epithelial cells was apparent. This effect is interpreted as a consequence rather than a cause of thymocyte depletion from the cortex. This study indicates that TCDD exposure affects the cortical epithelium of the rat thymus at a high dose level. Electron microscopy reveals that the differentiation of epithelial cells is altered. In addition, epithelial cell aggregates are formed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01969269
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  • 32
    Digitale Medien
    Digitale Medien
    Molecular genetics of human androgen insensitivity (1993)
    Springer
    European journal of pediatrics 152 (1993), S. S62 
    Details
    ISSN: 1432-1076
    Schlagwort(e): Androgen ; Receptor ; Genetics ; Mutations ; Human
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract Androgen insensitivity syndromes represent one cause of human male pseudohermaphroditism related to defects in the androgen receptor. The formation of a biologically active androgen receptor complex with testosterone and 5α-dihydrotestosterone is required for normal androgen action during fetal development and fifferentiation of the internal accessory sex glands and external genitalia. Cloning of the human androgen receptor complementary DNA and genetic screening of human subjects with the clinical and biochemical features of androgen insensitivity using the polymerase chain reaction, denaturing gradient gel electrophoresis and nucleotide sequencing techniques have led to the identification of molecular defects in the androgen receptor. The complexity of phenotypic presentation by affected subjects with the complete or partial forms of androgen insensitivity is represented by the heterogeneity of androgen receptor gene mutations which include deletions and point mutations, with the latter causing, inappropriate splicing of RNA, premature termination of transcription and amino acid substitutions. The naturally occurring mutations in the androgen receptor of subjects with androgen insensitivity represent a base upon which we can increase our understanding of the structure and function of the androgen receptor in normal physiology, and disease.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02125442
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  • 33
    Digitale Medien
    Digitale Medien
    Identification of Naemacyclus minor hyphae within needle tissues of Pinus sylvestris by immunoelectron microscopy (1993)
    Springer
    Archives of microbiology 160 (1993), S. 265-272 
    Details
    ISSN: 1432-072X
    Schlagwort(e): Pinus sylvestris ; Naemacyclus minor ; Immunocytochemical identification ; Ultrastructure ; Plant-fungus interactions
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Cultural investigations revealed that Naemacyclus minor, Lophodermium seditiosum and Cenangium ferruginosum were the most frequent colonizers of asymptomatic and symptomatic Pinus sylvestris needles. Since ultrastructural observations showed that morphological features were not suitable to differentiate hyphae of N. minor from hyphae of other isolates, the on-section immunogold labelling technique was applied in combination with an anti-N. minor specific immunoserum. The specificity of this serum was tested against culture hyphae of all isolates. Anti-N. minor specific immunoserum was then used to identify N. minor hyphae in thin sections of green P. sylvestris needles. The infection loci identified were restricted to small tissue areas located in the vicinity of stomata. In the hypodermis, hyphae and endocell-containing hyphae were located within the lumina of host cells but outside the protoplast. The growth of hyphae from cell to cell occurred through pits. The hyphae spreat into the mesophyll intercellularly and continued with the intracellular colonization of moribund and dead mesophyll cells in a later stage of infection. The observed host-parasite interactions at cellular and ultrastructural level are discussed in connection with the still controversial interpretation of the pathogenicity of N. minor.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00292075
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  • 34
    Digitale Medien
    Digitale Medien
    Ultrastructure of Bacillus pulvifaciens (1993)
    Springer
    Archives of microbiology 159 (1993), S. 114-118 
    Details
    ISSN: 1432-072X
    Schlagwort(e): Bacillus pulvifaciens ; Vegetative cells ; Spotes ; Ultrastructure ; Electron microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The ultrastructure of vegetative cells and spores of Bacillus pulvifaciens was studied by CTEM and SEM methods. The vegetative cells are rods, 1.6–4.5 μm long and 0.4–0.6 μm wide, exhibiting typical ultrastructural features of Gram-positive bacteria. The spores are of ellipsoidal shape, 0.6×1.2 μm in size, with six longitudinal ribs reaching up to 130 nm in height. There are satelite ribs on both sides of the longitudinal ribs, reaching up to 20 nm in height. Between the longitudinal ribs, additional transversal ribs were observed in SEM. A special tubular layer, separating the outer and inner coat of the spores, was revealed in ultrathin sections. This layer seems to be a typical ultrastructural feature of Bacillus pulvifaciens spores.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00250269
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  • 35
    Digitale Medien
    Digitale Medien
    Development of quasi-multicellular bodies of Treponema denticola (1993)
    Springer
    Archives of microbiology 160 (1993), S. 206-213 
    Details
    ISSN: 1432-072X
    Schlagwort(e): Treponema denticola ; Spirochetes ; Ultrastructure ; Electron microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The formation of quasi-multicellular bodies of Treponema denticola was analysed using different electron microscopical methods. These bacteria could develop four different conformations: (i) normal helical forms; (ii) twisted spirochetes, forming plaits; (iii) twisted spirochetes, forming club-like structures; (iv) spherical bodies in different size. Treponemes within spherical bodies, plaits, and clubs proved to be enclosed in a common outer sheath in which the normal arrangement of their axial flagella was lost. The development of the quasi-multicellular bodies starting from the monoforme spirochetes was elucidated and this morphogenetic process is illustrated by a schematic drawing. Factors which might be involved in the induction of the structures are discussed and their possible pathogenetic importance is considered.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00249126
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  • 36
    Digitale Medien
    Digitale Medien
    Differences in morphine reinforcement property in two inbred rat strains: associations with cortical receptors, behavioral activity, analgesia and the cataleptic effects of morphine (1993)
    Springer
    Psychopharmacology 112 (1993), S. 183-188 
    Details
    ISSN: 1432-2072
    Schlagwort(e): Morphine ; Behavioral activity ; Analgesia ; Rat ; Self-administration ; Genetics
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract The purpose of the current study was to investigate genetic differences between two inbred strains of rats, Fisher-344 (F344/N) and Wistar Albino Glaxo (WAG/GSto), in a number of drug-naive and drug-related behaviors, including oral and intravenous morphine self-administration. F344/N and WAG/GSto rats differed in drug-naive behaviors such as nociception, rearing and sensitivity to lick suppression tests but did not differ in locomotor activity, ambulation or grooming behavior. F344/N rats were less sensitive to thermal stimuli as measured via tail-flick response, and more sensitive to the suppressive effects of intermittent shock in a lick suppression test. The F344/N rats demonstrated a significantly greater amount of rearing in open field tests but did not differ from WAG/GSto rats in locomotor activity, ambulation or grooming behavior. In addition to the behavioral results, naive F344/N and WAG/GSto rats were found to differ in μ and α2 receptor concentrations (F344/N〉WAG/GSto) and in 5HT2 and D2 affinity constants (WAG/GSto〉F344/N). These two inbred rat strains also differed in drug-related behaviors. F344/N rats showed significantly greater depression of locomotor activity at morphine 3 mg/kg than WAG/GSto rats. In addition, F344/N rats consumed significantly greater amounts of morphine in a two-bottle choice procedure and morphine maintained significantly greater amounts of behavior during intravenous self-administration sessions. Importantly, drug maintained behavior was significantly greater than with vehicle only in the F344/N rats during operant self-administration sessions.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02244908
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  • 37
    Digitale Medien
    Digitale Medien
    Linkage studies of psychiatric disorders (1993)
    Springer
    European archives of psychiatry and clinical neuroscience 243 (1993), S. 143-149 
    Details
    ISSN: 1433-8491
    Schlagwort(e): Genetics ; Linkage ; Psychiatric disorders ; Genetic epidemiology
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary Linkage analysis has been successful in identifying the genetic basis of numerous Mendelian diseases. These successes were due in part to the rapid developments in molecular biology, which have yielded a plethora of informative genetic markers. Although there is strong evidence that the manifestation of schizophrenia and bipolar affective disorders is controlled by genes, no evidence for linkage has been established. For psychiatric disorders, the most important limiting factor is likely to be the lack of single loci with very large effects that occur with any relevant frequency. The difficulties of linkage studies in psychiatric disorders are discussed with reference to non-psychiatric genetic diseases for which linkage to genetic markers has been successful. Recommendations for collecting information to clarify the patterns of transmission of the psychiatric disorders are described.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02190720
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  • 38
    Digitale Medien
    Digitale Medien
    Identification of the phenotype in psychiatric genetics (1993)
    Springer
    European archives of psychiatry and clinical neuroscience 243 (1993), S. 131-142 
    Details
    ISSN: 1433-8491
    Schlagwort(e): Genetics ; Nosology ; Methodology ; Linkage analysis
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary Statistical procedures and molecular genetic techniques have attained a fine degree of resolution. Their ability to find disease genes has revolutionized medicine and raised hopes for breakthroughs in psychiatry. However, such breakthroughs may require an equally discriminating nosology. A psychiatric genetic nosology seeks to classify patients into categories that correspond to distinct genetic entities by addressing the problem of diagnostic accuracy: the degree to which a diagnosis correctly classifies people with and without a putative genetic illness. We review methods that deal with misclassification in genetic studies. These are clinical and epidemiological approaches that deal directly with how to define the observable manifestation of a putative genotype. We discuss two groups of methods: those that use known phenotypes and those that design new phenotypes.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02190719
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  • 39
    Digitale Medien
    Digitale Medien
    Genetic analysis of salinity tolerance in rice (Oryza sativa L.) (1993)
    Springer
    Theoretical and applied genetics 86 (1993), S. 333-338 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Genetics ; Rice ; Salinity ; Tolerance ; Na-Kratio ; Diallel
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The genetics of salinity tolerance in rice was investigated by a nine-parent complete diallel including reciprocals. Test materials involved susceptible (IR28, IR29, and MI-48), moderately tolerant (IR4595-4-1-13, IR9884-54-3-1E-P1, and IR10206-29-2-1), and tolerant (“Nona Bokra”, “Pokkali”, and SR26B) parents. Twoweek-old seedlings were grown in a salinized (EC = 12 dS/m) culture solution for 19 days under controlled conditions in the IRRI phytotron. Typical characteristics of salinity tolerance in rice were found to be Na+ exclusion and an increased absorption of K+ to maintain a good Na-K balance in the shoot. Genetic component analysis (GCA) revealed that a low Na-K ratio is governed by both additive and dominance gene effects. The trait exhibited overdominance, and two groups of genes were detected. Environmental effects were large, and the heritability of the trait was low. Our findings suggest that when breeding for salt tolerance, selection must be done in a later generation and under controlled conditions in order to minimize environmental effects. Modified bulk and single-seed descent would be the suitable breeding methods. Combining ability analysis revealed that both GCA and specific combining ability (SCA) effects were important in the genetics of salt tolerance. Moderately tolerant parents — e.g., IR4595-4-1-13 and IR9884-54-3-1E-P1 — were the best general combiners. Most of the best combinations had susceptible parents crossed either to moderate or tolerant parents. The presence of reciprocal effects among crosses necessitates the use of susceptible parents as males in hybridization programs. Large heterotic effects suggest the potential of hybrid rice for salt-affected lands.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00222098
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  • 40
    Digitale Medien
    Digitale Medien
    Quantitative and qualitative trait loci affecting host-plant response to Exserohilum turcicum in maize (Zea mays L.) (1993)
    Springer
    Theoretical and applied genetics 87 (1993), S. 537-544 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Genetics ; Disease ; Mapping ; Breeding
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Molecular markers at 103 loci were used to identify the location of quantitative sources of resistance to Exserohilum turcicum in 150 F2∶3 lines of a B52/Mo17 maize population. Host-plant response was measured in terms of the average number of lesions per leaf, the average percent leaf tissue diseased (severity), and the average size of lesions. The location of quantitative trait loci were compared with three loci having known qualitative effects, namely Ht1, Ht2 and bx1. Chromosomal regions containing the Ht1 and Ht2 loci showed a small contribution in determining lesion size, even though alleles with dominant, qualitative effects at these loci have never been reported in either inbred parent. Similar effects were not observed for the number of lesions or for disease severity. Likewise, some contribution was observed for chromosomal regions encompassing the bx1 locus in determining lesion size but not the number of lesions or disease severity. Overall the contribution of loci in the vicinity of Ht1, Ht2 and bx1 was small relative to variation attributable to loci with quantitative effects identified in this study. Molecular-marker-facilitated mapping concurred with previous reciprocal translocation mapping studies on the importance of chromosomes 3, 5 and 7, despite the fact that these studies utilized diverse sources of resistant germplasm.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00221876
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  • 41
    Digitale Medien
    Digitale Medien
    Avian epidermis contains ATPase- and Ia-positive Langerhans-like cells (1993)
    Springer
    Cell & tissue research 271 (1993), S. 103-106 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Skin ; Langerhans cell ; ATPase-histochemistry ; Ultrastructure ; Ia antigen ; Four avian species
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The occurrence of cells resembling mammalian Langerhans cells in the avian epidermis was studied by ATPase histochemistry, Ia immunoreactivity and electron microscopy. The existence of MHC class II antigen-(Ia) expressing, ATPase-positive dendritic cells, which are ultrastructurally similar to mammalian Langerhans cells except for the absence of Birbeck granules, was demonstrated. These cells may be a basic component of the immune system of birds.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00297547
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  • 42
    Digitale Medien
    Digitale Medien
    Development of the diffuse endocrine system in the chicken proventriculus (1993)
    Springer
    Cell & tissue research 271 (1993), S. 107-113 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Proventriculus ; Endocrine ontogenesis ; Ultrastructure ; Regulatory peptides ; Immunocytochemistry ; Silver impregnations ; Chicken
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The development of endocrine cells in the chicken proventriculus has been investigated using light-and electron-microscopy in conjunction with silver and immunocytochemical techniques. The first morphologically detectable endocrine cells were found in 5-day-old embryos by electron microscopy. From the 9th to the 13th day, endocrine cells in contact with the lumen of the organ could be detected both by electron and light (silver impregnation) microscopy. The number of open-type endocrine cells progressively decreased and the number of closed-type increased after this stage. Until the 16th day, endocrine cells were located exclusively in the luminal epithelium, but afterwards they appeared in progressively greater numbers in the compound glands. After hatching, long cytoplasmic processes could be seen in the endocrine cells. Immunoreactivities to regulatory substances appeared in the following order: serotonin (day-14), avian pancreatic polypeptide, glucagon and somatostatin (day-16), bombesin and neurotensin (day-18), and finally, met-enkephalin (day-21).
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00297548
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  • 43
    Digitale Medien
    Digitale Medien
    Immunocytochemical effects of thyroxine stimulation on the adenohypophysis of dwarf (dw) mutant mice (1993)
    Springer
    Cell & tissue research 274 (1993), S. 579-585 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Thyroxine ; Pituitary gland, pars distalis ; Immunocytochemistry ; Ultrastructure ; Mouse (Snell dwarf)
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Abstract The effects of dietary thyroxine on the immunoreactivity of cells in the pars distalis of the adenohypophysis in dwarf (dw/dw) mice were determined by ultrastructural immunocytochemistry. In nontreated dwarfs only adrenocorticotropic hormone (ACTH) cells and luteinizing hormone (LH) cells showed positive reactions to their respective antibodies, whereas no cells showed immunoreactivity to antibodies to growth hormone (GH), thyroid-stimulating hormone (TSH), or prolactin (Prl). In dwarfs supplemented postnatally with dietary thyroxine for 9 wks, the treatment failed to produced immunoreactive GH, TSH or Prl cells. However, LH cells became more prominent and fully developed, with denser concentrations of immunoreactive particles overlying the secretory granules than occurred in nontreated dwarfs. In thyroxine-treated dwarfs, ACTH cells were similar in ultrastructural features and immunoreactivity to those in nontreated dwarfs.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00314556
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  • 44
    Digitale Medien
    Digitale Medien
    Biphasic effect of ACTH on growth of rat adrenocortical cells in primary culture (1993)
    Springer
    Cell & tissue research 271 (1993), S. 169-176 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Adrenal cortex ; Differentiation ; Tissue culture ; Proliferation ; Ultrastructure ; Steroids ; Rat (Sprague Dawley)
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The proliferation rate of differentiating fetal rat adrenocortical cells was studied in primary culture. In this system, stimulation with ACTH induces differentiation of zona glomerulosa-like cortical cells into zona fasciculata-like cells. Incorporation of bromodeoxyuridine (BrdU) was studied immunocytochemically by use of anti-BrdU antibody, and the proliferation rate was counted from the monolayer colonies of adrenocortical cells. After 21 days of cultivation in the absence of ACTH, the proliferation rate of zona glomerulosa-like cells was 10%. The rate slowly declined to 1% at the age of 100 days during continuous cultivation in the absence of ACTH. Stimulation with ACTH induced a strong inhibition in the proliferation rate (down to 2% during the first 24 h). Treatment with ACTH during the following 48 h led to an extremely intense proliferation of adrenocortical cells at a proliferation rate of 25%. Continuous treatment with ACTH up to 100 days led to a persistent growth of adrenocortical cells, and a proliferation rate over 2-fold higher than in control cells cultivated in the absence of ACTH. Thus, ACTH is the principal growth-promoting factor also in vitro, as has been found in in vivo studies. This growth effect is mediated by a biphasic course; at the beginning of differentiation the effect is inhibitory and is followed by a persistent stimulation of the growth of adrenocortical cells.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00297555
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  • 45
    Digitale Medien
    Digitale Medien
    Ultrastructure and morphometry of testicular Leydig cells and the interstitial components correlated with testosterone in aging rats (1993)
    Springer
    Cell & tissue research 271 (1993), S. 241-255 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Leydig cell ; Testis ; Interstitium ; Aging ; Ultrastructure ; Morphometry ; Testosterone ; Rat (Wistar)
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Abstract The ultrastructure of testicular interstitium in young and aged adult rats was analysed using morphometric methods, and the plasma testosterone concentration was measured. With increasing age there was an augumentation in the volume of collagen fibrils in the intercellular matrix and in blood vessels. During the aging process (approximately two years) the average volume of the Leydig cell decreased from 1364 μm3 to 637 μm3, but the number of Leydig cells in paired testes increased from 53x106 to 113x106. The absolute volume of smooth surfaced endoplasmic reticulum (SER) per Leydig cell amounted in aged rats to 78% of that in young adult rats. The total amount of SER in paired testes increased by 62% with aging. The present analysis suggests that the ability of SER to maintain peripheral testosterone concentration decreases with age. In young adult rats the absolute volume of peroxisomes per Leydig cell correlated significantly with the concentration of testosterone in blood and also with the absolute volume of SER per Leydig cell. These results combined with ultrastructural observations of close apposition of peroxisomes and SER suggest that peroxisomes have a role in testosterone secretion by Leydig cells.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00318610
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  • 46
    Digitale Medien
    Digitale Medien
    Effect of omeprazole-evoked hypergastrinemia on ultrastructure of enterochromaffin-like cells in the stomach of portacaval-shunted rats (1993)
    Springer
    Cell & tissue research 272 (1993), S. 71-77 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Enterochromaffin-like cells ; Hypergastrinemia ; Hypertrophy ; Ultrastructure ; Omeprazole ; Portacaval shunt ; Rat (Sprague Dawley)
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Abstract The histamine-producing enterochromaffinlike (ECL) cells in the acid-producing portion of the rat stomach responded to long-standing hypergastrinemia (omeprazole treatment daily for 8–10 weeks) with hypertrophy (and hyperplasia) and with a reduced number of granules and vesicles per unit cytoplasm. There was a reduction in the ratio of electron-dense granules versus vesicles and an increase in the profile diameter of the vesicles. Also, portacaval shunting (PCS) induced changes in the ECL cells, manifesting (i) as an increase in cell number and size, and (ii) as a reduced number of granules and vesicles per unit area. The cytoplasmic granules and vesicle profiles were enlarged, and the ratio of granules versus vesicles was reduced. The combination of PCS and long-standing hypergastrinemia (omeprazole treatment) produced a greatly enhanced ECL cell hypertrophy (and hyperplasia) and a marked reduction in the number of granules. The ratio of granules versus vesicles was markedly reduced while the profile diameters of both granules and vesicles were increased. The relative predominance of very large vesicles (vacuoles) was a prominent feature of the ECL cells in these rats.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00323572
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  • 47
    Digitale Medien
    Digitale Medien
    Ultrastructure of free nerve endings in respiratory and squamous epithelium on the rat nasal septum (1993)
    Springer
    Cell & tissue research 274 (1993), S. 329-335 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Intraepithelial axons ; Free nerve endings ; Nasal mucosa ; Ultrastructure ; Enzyme histochemistry ; Immunocytochemistry ; Rat (Wistar)
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Abstract The distribution of nerve fibres in the mucosa of the nasal septum of the rat was investigated by means of transmission electron microscopy on transverse and tangential ultrathin sections. Near the basement membrane of respiratory and squamous epithelium, a rather dense network of unmyelinated nerve fibres occurs. Some fibres in the respiratory epithelium ascend between the epithelial cells to reach up to the tight junctions. These fibres appeared in transverse sections to end as hooks or boutons, sometimes with branches. These shapes resemble the free nerve endings that are considered to act as nociceptors. The small intraepithelial fibres, with diameters of about 0.5–1 μm, contain both dense granules and clear vesicles comparable to synaptic vesicles. Substance P was found in dense granules in basal fibres; vasoactive intestinal peptide was absent throughout the epithelium. Acetylcholinesterase activity was observed closely associated with the basal fibres; the apical fibres showed little if any activity. Membrane specializations pointing to an efferent function as well as structures usually associated with mechanoreceptive functions were lacking in both respiratory and squamous epithelium.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00318751
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  • 48
    Digitale Medien
    Digitale Medien
    The effects of gonadectomy and testosterone treatment on the Harderian gland of the green frog, Rana esculenta (1993)
    Springer
    Cell & tissue research 273 (1993), S. 201-208 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Harderian gland ; Castration ; Testosterone ; Ultrastructure ; Rana esculenta (Anura)
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Abstract The effects of gonadectomy and testosterone treatment on the fine structure of the Harderian gland in male and female green frogs were investigated in different periods of the year. Gonadectomy, carried out when the glands are in the lowest secretory phase (September), causes degenerative changes consisting of a reduction of the rough endoplasmic reticulum, the appearance of autolysosomes, and an increase of nuclear heterochromatin. These effects can be prevented by testosterone treatment. No castration effects are found during the recovery (November) and enhancement (April-May) phases of secretory activity. The results suggest that the frog Harderian gland's sensitivity to testosterone changes during the annual cycle. The androgen dependence of the Harderian gland is correlated with the presence of androgen receptors in both male and female forgs.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00312821
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  • 49
    Digitale Medien
    Digitale Medien
    Carbamylcholine-induced morphological changes and spatial dynamics of [Ca2+]c in Harderian glands of guinea pigs: calcium-dependent lipid secretion and contraction of myoepithelial cells (1993)
    Springer
    Cell & tissue research 274 (1993), S. 1-14 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Harderian gland ; Ultrastructure ; Fura-2/AM digital imaging ; Calcium ion ; Carbamylcholine ; Myoepithelial cells ; Guinea pig
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Abstract To determine whether lipid-secreting cells have cytosolic Ca2+ concentration ([Ca2+]c)-related secretory mechanisms, morphological changes and intracellular calcium dynamics of Harderian glands of guinea pigs stimulated by secretagogs were studied by electron microspy and Fura-2/AM digital image analysis. Control glandular cells contained large lipid vacuoles that were bordered by multi-layered membranes. Rough-surfaced endoplasmic reticulum, mitochondria, and smooth-surfaced endoplasmic reticulum may be involved in lipid vacuole formation. Myoepithelial cells surrounded alveoli. After carbamylcholine (CCh, 10−6, 10−5, and 10−3 M) stimulation, lipid materials within the membranous structures were frequently discharged by an exocytotic mechanism. Conspicuous deformation of glandular cells caused by vigorous contraction of myoepithelial cells was observed in isolated alveoli after 10−6M CCh stimulation, whereas the deformaties of glandular tissues perfused via vessels were small even after 10−3M CCh stimulation. Connective tissue between glandular alveoli inhibited unbridled myoepithelial-cell contraction. Fura-2/AM digital imaging analysis revealed that CCh stimulation caused an increase in [Ca2+]c in isolated alveoli. The morphological reactions and changes in [Ca2+]c were prevented by atropine. When extracellular calcium ions were absent, enhanced extrusion of lipid vacuoles, myoepithelial-cell contraction, and a rise in [Ca2+]c after CCh stimulation were not observed. Nicotine and catecholamines had no effect on the secretion or on the dynamics of [Ca2+]c. It can be concluded that acetylcholine elicits exocytosis in glandular cells and contraction of the myoepithelial cells of Harderian glands, accompanied by an increase in [Ca2+]c. The dynamics of [Ca2+]c of the gland alveoli are mostly dependent on extracellular Ca2+.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00327979
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  • 50
    Digitale Medien
    Digitale Medien
    Electron-microscopic immunocytochemical study of the endocrine pancreas of sea bass (Dicentrarchus labrax) (1993)
    Springer
    Cell & tissue research 274 (1993), S. 303-314 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Endocrine pancreas ; Peptides ; Ultrastructure ; Immunogold labeling ; Sea bass ; Dicentrarchus labrax ; (Teleostei)
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Abstract Insulin (B)-, somatostatin 25 (SST-25) (D1)-, somatostatin 14 (SST-14) (D2)-, glucagon (A)-, and glucagon PP/PYY/NPY (PP-like)-immunoreactive cells in islets of sea bass (Dicentrarchus labrax) were characterized according to their ultrastructure and immunogold labeling. Cells labeled with antisera to bonito and salmon insulin had numerous secretory granules with a small halo and round core, and a few with wide halo and round or crystalloid core. Gold particles were found throughout the granule in tissue labeled with the former but only in the core in tissue labeled with the latter. D1 cells had large granules with a medium electron-dense content and some with a darker core. D2 had smaller medium or high electron-dense secretory granules than D1 cells, located mainly in cell periphery. Glucagon-immunoreactive cells contained some granules with a polygonal core that was heavily labeled and other granules with a round core with no or hardly any labeling. Glucagon and PP-like immunoreactivity were co-localized in secretory granules, in which the gold particles showed no different distribution with the various antisera used. PYY-immunoreactive granules were also found in nerve endings. All the pancreatic endocrine cell types showing involutive characteristics are found.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00318749
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  • 51
    Digitale Medien
    Digitale Medien
    Cutaneous and subcutaneous sensory receptors of the hagfish Myxine glutinosa with special respect to the trigeminal system (1993)
    Springer
    Cell & tissue research 274 (1993), S. 353-366 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Trigeminal nerve ; Mechanoreceptors ; Skin ; Peripheral autonomic ganglia ; Ultrastructure ; Myxine glutinosa ; Cyclostomata
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Abstract The integument of the hagfish Myxine glutinosa is described with respect to the topography and the fine structural organization of the dermal and hypodermal nerve fiber plexus. Both nerve fiber plexuses contain small ganglion cells with axodendritic and axosomatic synapscs. The six barbels of the head (4 nasal and 2 oral barbels) are supplied with about 5600 afferent trigeminal nerve fibers via the right and left ophthalmic nerve. With respect to the topography of the sensory nerve terminals in the barbels different types of receptors are termed the external cuff receptor, internal cuff receptor, and perichondrial receptor. Free nerve terminals occur within the epidermal layer, especially at the tip region of the barbels and in the glassy membrane of the dermis. The hypodermal edge receptor organ extends from the ventral nasal barbel to the oral barbel. A mechanoreceptive function of the different receptor types is discussed. The innervation pattern of the barbel is similar to the innervation of the mammalian sinus hair. In this context, the barbel is a highly differentiated receptor organ able to explore the nearest surroundings with high stereognostic perception. The ganglion cells of the skin seem to represent a part of the peripheral autonomic nervous system, which is involved in the control of secretion mechanisms.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00318754
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  • 52
    Digitale Medien
    Digitale Medien
    Morphology and distribution of Müller cells in the retina of the toad Bufo marinus (1993)
    Springer
    Cell & tissue research 272 (1993), S. 183-192 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Retina ; Müller cells ; Neuron-specific enolase ; Immunocytochemistry ; Quantitative analysis ; Ultrastructure ; Bufo marinus (Anura)
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Abstract We have previously shown that an antibody against neuron-specific enolase (NSE) selectively labels Müller cells (MCs) in the anuran retina (Wilhelm et al. 1992). In the present study the light- and electron-microscopic morphology of MCs and their distribution were described in the retina of the toad, Bufo marinus, using the above antibody. The somata of MCs were located in the proximal part of the inner nuclear layer and were interconnected with each other by their processes. The MCs were uniformly distributed across the retina with an average density of 1500 cells/mm2. Processes of MCs encircled the somata of photoreceptor cells isolating them from each other by glial sheath, except for those of the double cones. Some of the photoreceptor pedicles remained free of glial sheath. Electron-microscopic observations confirmed that MC processes provide an extensive scaffolding across the neural retina. At the outer border of the ganglion cell layer these processes formed a non-continuous sheath. The MC processes traversed through the ganglion cell layer and spread beneath it between the neuronal somata and the underlying optic axons. These processes formed a continuous inner limiting membrane separating the optic fibre layer from the vitreous tissue. Neither astrocytic nor oligodendrocytic elements were found in the optic fibre layer. The significance of the uniform MC distribution and the functional implications of the observed pattern of MC scaffolding are discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00323585
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  • 53
    Digitale Medien
    Digitale Medien
    Structure of the tertiary component of the myenteric plexus in the guinea-pig small intestine (1993)
    Springer
    Cell & tissue research 272 (1993), S. 509-516 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Myenteric plexus ; Enteric nervous system ; Intestine, small ; Ultrastructure ; Innervation, of intestinal muscle ; Guinea-pig
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Abstract The tertiary component of the myenteric plexus consists of interlacing fine nerve fibre bundles that run between its principal ganglia and connecting nerve strands. It was revealed by zinc iodide-osmium impregnation and substance P immunohistochemistry at the light-microscope level. The plexus was situated against the inner face of the longitudinal muscle and was present along the length of the small intestine at a density that did not vary markedly from proximal to distal. Nerve bundles did not appear to be present in the longitudinal muscle as judged by light microscopy, although numberous fibre bundles were encountered within the circular muscle layer. At the ultrastructural level, nerve fibre bundles of the tertiary plexus were found in grooves formed by the innermost layer of longitudinal smooth muscle cells. In the distal parts of the small intestine, some of these nerve fibre bundles occasionally penetrated the longitudinal muscle coat. Vesiculated profiles in nerve fibre bundles of the tertiary plexus contained variable proportions of small clear and large granular vesicles; they often approached to within 50–200 nm of the longitudinal smooth muscle cells. Fibroblast-like cells lay between strands of the tertiary plexus and the circular muscle but were never intercalated between nerve fibre varicosities and the longitudinal muscle. These anatomical relationships are consistent with the tertiary plexus being the major site of neurotransmission to the longitudinal muscle of the guinea-pig small intestine.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00318557
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  • 54
    Digitale Medien
    Digitale Medien
    Effects of elastase on contractility and morphology of elastic tissue in isolated guinea pig papillary muscles (1993)
    Springer
    Heart and vessels 8 (1993), S. 71-78 
    Details
    ISSN: 1615-2573
    Schlagwort(e): Elastase ; Elastin ; Contractile tension ; Guinea pig papillary muscle ; Ultrastructure
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary Elastase (ELA) is an enzyme catalyzing the digestion of elastin, an essential constituent of elastic fibers. Using isolated guinea pig papillary muscles, we examined the effect of ELA (3 × 10−7 −3 × 10−4 g/ml) on resting tension (RT) and twitch tension (TT). The effects of ELA on elastic fibers located in the subendocardium were examined histologically. A relatively high concentration of ELA (3 × 10−4 g/ml) increased TT transiently, with progressive decreases in RT. In contrast, a relatively low concentration (3 × 10−5 g/ml) decreased both TT and RT straightforwardly. Much lower concentrations (3 × 10−6 −3 × 10−7 g/ml) did not reveal significant effects. The ELA-induced increases in TT were unaffected in the presence of atenolol (10−5 g/ml), ouabain (10−7M) or ryanodine (10−6M). ELA did not increase the maximum rate of rise of slow action potentials recorded using standard microelectrodes.ELA (3 × 10−5 g/ml) decreased the maximum TT obtained at optimal RT or Lmax, and decreased the slope of the ascending and descending limbs of the TT-RT relation curve (Frank-Starling's). Electronmicroscopic findings revealed that subendocardial elastin was mostly digested at ELA concentrations of 3 × 10−5 −3 × 10−4 g/ml. These findings suggest that the decrease of RT by ELA may be, at least in part, caused by a decomposition of the elastic fibers. On the other hand, the increase of TT by ELA could not be attributed to a release of endogenous catecholamine, an inhibition of Na+, K+-pump, a release of Ca2+ from sarcoplasmic reticulum, or an increase of slow inward current.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01744386
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  • 55
    Digitale Medien
    Digitale Medien
    Sub-cellular distribution of nitrogen compounds inAzolla andAnabaena by ESI and EELS analysis (1993)
    Springer
    Protoplasma 173 (1993), S. 158-169 
    Details
    ISSN: 1615-6102
    Schlagwort(e): Azolla-Anabaena symbiosis ; Ultrastructure ; Nitrogen distribution ; Electron spectroscopic imaging ; Electron energy loss spectroscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The sub-cellular localization of some nitrogen compounds within the leaf cavities ofAzolla filiculoides Lam. was obtained by means of electron spectroscopic imaging (ESI) and electron energy loss spectroscopy (EELS). The analyses were performed on ultrathin unstained sections of differentAzolla leaf cavities which contain epidermal hairs,Anabaena azollae Strasb. and bacteria. Net nitrogen distributions were visualized by image analysis, and nitrogen peaks were evidenced in spectra recorded in the same areas. Different distributions of nitrogen compounds were observed within the leaf cavities along the stem, in particular inside the epidermal hairs ofAzolla and the vegetative cells and heterocysts ofA. azollae.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01379004
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  • 56
    Digitale Medien
    Digitale Medien
    Effect of cytochalasin A on actin distribution in the fission yeastSchizosaccharomyces pombe studied by fluorescent and electron microscopy (1993)
    Springer
    Protoplasma 176 (1993), S. 24-32 
    Details
    ISSN: 1615-6102
    Schlagwort(e): Actin ; Cytoskeleton ; Schizosaccharomyces pombe ; Freeze substitution ; Ultrastructure
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Actin distribution and ultrastructure of the fission yeastSchizosaccharomyces pombe treated with cytochalasin A (CA) were investigated by fluorescence microscopy using rhodamine-conjugated phalloidin (rh-ph) and freeze substitution electron microscopy. Among the cytochalasins tested, CA was most effective and at 5 μg/ ml inhibited the appearance of the actin ring at the cell equator at the stage prior to septum formation and the accumulation of actin dots at the septum-forming site both in wild-type cells and the mutantcdc 11, which is defective in septum formation at restrictive temperature. Freeze substitution electron microscopy of CA-treated cells revealed the displacement and morphological alteration of cytoplasmic vesicles and dictyosomes within 30 min and the appearance of dense bodies in the cytoplasm. A sub-population of cytoplasmic vesicles and dictyosomes were insensitive to CA and maintained their original structure. An electron less dense layer containing filamentous material was noted beneath the plasma membrane and thought to be the area of heavy actin patches stained with rh-ph at the cells ends. These results suggest that CA disrupted an actin network that normally maintains the organization of the secretory pathway involving dictyosomes and vesicles.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01378936
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  • 57
    Digitale Medien
    Digitale Medien
    Membrane coatings on the generative cell surface of freeze-substituted monocotyledon pollen (1993)
    Springer
    Protoplasma 176 (1993), S. 84-88 
    Details
    ISSN: 1615-6102
    Schlagwort(e): Pollen (high-pressure freezing) ; Freeze-substitution ; Generative cell ; Plasma membrane ; Ultrastructure
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary High-pressure freezing/freeze-substitution/TEM were employed for studying plasma membrane coatings in mature pollen grains. Data are presented on 6 species belonging to 4 not closely related orders. Two sorts of coatings were observed at the cytoplasmic face of the vegetative plasma membrane bordering the generative cell: (i) A dense, uniform layer with an average thickness of 10 nm (“fluffy coating”). (ii) “Strip-shaped projections” protruding rectangularly into the vegetative cytoplasm. They are 25 to 35 nm in height and set at a constant distance of about 30 nm from each other. Their maximum length, estimated from grazing sections, is about 400 nm. This is the first description of periodically arranged plasma membrane protrusions in freeze-substituted plant cells. Both kinds of membrane coatings are species-and age-dependent features. Especially “strip-shaped projections” are frequently intimately associated with polymorphic endoplasmic reticulum surrounding the generative cell, a hitherto unreported pattern. It is presumed that “strip-shaped projections” are similar to, or even identical with, “ordered ridges” observed in freeze-fracturedPhoenix dactylifera pollen.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01378942
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  • 58
    Digitale Medien
    Digitale Medien
    The generative cell and its close association with the endoplasmic reticulum of the vegetative cell in pollen ofCyrtandra pendula (Gesneriaceae) (1993)
    Springer
    Protoplasma 177 (1993), S. 73-81 
    Details
    ISSN: 1615-6102
    Schlagwort(e): Cyrtandra pendula ; Generative cell ; Endoplasmic reticulum ; Pollen grain ; Ultrastructure
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The development of the generative cell (GC) was examined in the bicellular pollen ofCyrtandra pendula. The essential stages are: (1) GC attached to the inline, (2) GC detached and spheroidal in shape, (3) GC mature and elongated. Cisternae of the endoplasmic reticulum (ER) of the vegetative cell are in close contact with the GC at all stages of development. In stages (1) and (2) the entire, slightly undulated surface of the GC is surrounded by tightly appressed, single ER tubules or short stacks. In mature pollen grains (stage 3) the shape of the GC as well as the arrangement of the surrounding ER changes conspicuously. The GC is now spindleshaped and its surface is wrinkled. An ER tubule is present in each invagination. These ER tubules form a cage-like framework around the GC. In the cytoplasm of the generative cell, 6 to 7 microtubular bundles with longitudinal orientation can be observed. They seem to be responsible for maintaining the elongate shape of the GC. During all stages of development vegetative ER cisternae are the only elements intimately associated with the GC wall. This feature indicates that the ER may contribute to the formation of the undulated outer shape of the GC. Also discussed is the possibility that energy-carrying substances are conveyed into the GC through the channels of ER.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01403401
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  • 59
    Digitale Medien
    Digitale Medien
    Microspore development inBrassica napus and the effect of high temperature on division in vivo and in vitro (1993)
    Springer
    Protoplasma 172 (1993), S. 154-165 
    Details
    ISSN: 1615-6102
    Schlagwort(e): Brassica napus ; Cell division ; High temperature ; Microspore ; Embryogenesis ; Ultrastructure
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Brassica napus cv. Topas microspores isolated and cultured near the first pollen mitosis and subjected to a heat treatment develop into haploid embryos at a frequency of about 20%. In order to obtain a greater understanding of the induction process and embryogenesis, transmission electron microscopy was used to study the development of pollen from the mid-uninucleate to the bicellular microspore stage. The effect of 24 h of high temperature (32.5 °C) on microspore development was examined by heat treating microspore cultures or entire plants. Mid-uninucleate microspores contained small vacuoles. Late-uninucleate vacuolate microspores contained a large vacuole. The large vacuole of the vacuolate stage was fragmented into numerous small vacuoles in the late-uninucleate stage. The late-uninucleate stage contained an increased number of ribosomes, a pollen coat covering the exine and a laterally positioned nucleus. Prior to the first pollen mitosis the nucleus of the lateuninucleate microspore appeared to be appressed to the plasma membrane; numerous perinuclear microtubules were observed. Microspores developing into pollen divided asymmetrically to form a large vegetative cell with amyloplasts and a small generative cell without plastids. The cells were separated by a lens-shaped cell wall which later diminished. At the late-bicellular stage the generative cell was observed within the vegetative cell. Starch and lipid reserves were present in the vegetative cell and the rough endoplasmic reticulum and Golgi were abundant. The microspore isolation procedure removed the pollen coat, but did not redistribute or alter the morphology of the organelles. Microspores cultured at 25 °C for 24 h resembled late-bicellular microspores except more starch and a thicker intine were present. A more equal division of microspores occurred during the 24 h heat treatment (32.5 °C) of the entire plant or of cultures. A planar wall separated the cells of the bicellular microspores. Both daughter cells contained plastids and the nuclei were of similar size. Cultured embryogenie microspores contained electron-dense deposits at the plasma membrane/cell wall interface, vesicle-like structures in the cell walls and organelle-free regions in the cytoplasm. The results are related to embryogenesis and a possible mechanism of induction is discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01379373
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  • 60
    Digitale Medien
    Digitale Medien
    Improved mating efficiency inAcetabularia acetabulum: recovery of 48–100% of the expected zygotes (1993)
    Springer
    Protoplasma 176 (1993), S. 53-63 
    Details
    ISSN: 1615-6102
    Schlagwort(e): Acetabularia acetabulum ; Gamete release ; Mating efficiency ; Mating physiology ; Gamete half-life ; Genetics
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary We have improved zygote recovery 11–1,000 fold by optimizing the physiology of gamete release and mating inAcetabularia acetabulum. Gamete release was affected by agar purity, concentration, and volume/gametangial pair. Cold pre-treatment of gametangia (14–30 d at 10°C in the dark) synchronized subsequent gamete release at 21°C in the light. Cold pre-treatment was nearly twice as effective in synchronizing subsequent gamete release when intact, gametangia-bearing caps rather than isolated gametangia were pretreated. Synchronizing gamete release doubled mating efficiency. In a wild-type laboratory strain ofA. acetabulum, there were 1,561±207 gametes/gametangium which had half-lives of 14.5 d in 0.1% seawater-agar. We recovered 48–93% of the expected numbers of zygotes from a mass mating of 8 to 1,226 gametangia and 11–128% of the expected numbers of zygotes from mating single gametangial pairs: the large range in the calculated mating efficiency may be attributable to the variation in the numbers of gametes made per gametangium. Zygote recovery from single gametangial pairs was highly dependent on the volume of mating matrix. In addition, most zygotes recovered were unattached to any other zygotes in the subsequent generation (〉 95% single cells from matings of 1–500 gametangial pairs). Our improvements in mating conditions and zygote recovery (1) have facilitated cell manipulation and culture ofA. acetabulum in the laboratory; and (2) have made controlled crosses for selection and genetic analysis of mutants feasible. These advances have removed a major barrier to genetic analysis of development inAcetabularia.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01378939
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  • 61
    Digitale Medien
    Digitale Medien
    Early events in division of the generative cell ofOrnithogalum virens (1993)
    Springer
    Protoplasma 172 (1993), S. 77-83 
    Details
    ISSN: 1615-6102
    Schlagwort(e): Ornithogalum virens ; Generative cell ; Mitosis ; Pollen ; Ultrastructure
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Ornithogalum virens is a bicellular pollen species. In mature pollen, the generative nucleus is at advanced prophase. Mitosis of the generative cell is resumed just after pollen rehydration and prometaphase occurs within 10 min of germination. Prometaphase is manifested by nuclear envelope breakdown and the appearance of spindle microtubules in the nucleoplasm region. At this stage the number of cytoplasmic microtubules located in the generative cell periphery appears to decrease. Endoplasmic reticulum-like cisternae originating from the nuclear envelope tend to be spaced around the chromosomes, outside the area of the forming mitotic spindle. Some also begin to penetrate the spindle area. The results are discussed in terms of the generative cell cycle in bicellular pollen.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01379365
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  • 62
    Digitale Medien
    Digitale Medien
    Ultrastructure of transfer cells in spontaneous nodules of alfalfa (Medicago sativa) (1993)
    Springer
    Protoplasma 172 (1993), S. 64-76 
    Details
    ISSN: 1615-6102
    Schlagwort(e): Alfalfa ; Spontaneous nodules ; Development ; Rhizobium meliloti ; Transfer cells ; Ultrastructure
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Spontaneous nodules were formed on the primary roots of alfalfa plants in the absence ofRhizobium. Histologically, these white single-to-multilobed structures showed nodule meristems, cortex, endodermis, central zone, and vascular strands. Nodules were devoid of bacteria and infection threads. Instead, the larger cells were completely filled with many starch grains while smaller cells had very few or none. Xylem parenchyma and phloem companion cells exhibited long, filiform and branched wall ingrowths. The characteristic features of both types of transfer cells were polarity of wall ingrowths, high cytoplasmic density, numerous mitochondria, abundant ribosomes, well-developed nucleus and nucleolus, and vesicles originated from rough endoplasmic reticulum. These results were compared with normal nodules induced byRhizobium. Our results suggest that xylem parenchyma and phloem companion transfer cells are active and probably involved in the short distance transport of solutes in and out of spontaneous nodules. Since younger nodules showed short, papillate, and unbranched wall ingrowths, and older tissue showed elongated, filiform and branched wall ingrowths, the development of wall ingrowths seemed to be gradual rather then abrupt. The occurrence of both type-A and -B wall ingrowths suggests that phloem companion transfer cells may be active in loading and unloading of sieve elements. Since there were no symbiotic bacteria and thus no fixed nitrogen, it is tempting to speculate that xylem parenchyma transfer cells may be re-transporting accumulated carbon from starch grains to the rest of the plant body by loading xylem vessels. Fusion of ER-originated vesicles with wall ingrowth membrane indicated the involvement of ER in the membrane formation for elongating wall ingrowths. Since transfer cells were a characteristic feature of both spontaneous andRhizobium-induced nodules, their occurrence and development is controlled by the genetic make-up of alfalfa plant and not by a physiological source or sink emanating from symbiotic bacteria.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01379364
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  • 63
    Digitale Medien
    Digitale Medien
    The effects of abscisic acid on the maturation ofBrassica napus somatic embryos (1993)
    Springer
    Protoplasma 174 (1993), S. 147-157 
    Details
    ISSN: 1615-6102
    Schlagwort(e): Abscisic acid ; Brassica napus ; Embryo maturation ; Reserves metabolism ; Somatic embryos ; Ultrastructure
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary A comparison of embryos, cultured for increasing periods of time with and without abscisic acid (ABA), was undertaken to investigate, at the ultrastructural level, the influence of this growth regulator on the maturation of rapeseed (Brassica napus) somatic embryos. In the absence of ABA, the embryos germinated precociously while lipid bodies (LB), which were not numerous, soon degraded, as revealed by a depletion process associated with the appearance of morphologically mature glyoxysomes and an increase in the number of mitochondria. Moreover, a lack of protein bodies indicated that storage protein accumulation was not initiated under these conditions. On the contrary, the addition of ABA (10 μM) induced marked modification of embryo metabolism. Indeed, ABA completely prevented precocious embryo germination and inhibited lipid reserve catabolism. Moreover, the formation of small vacuoles and proliferation of rough endoplasmic reticulum in their vicinity suggested the onset of storage protein accumulation. After 15 days in the presence of ABA, the embryos contained abundant lipid and protein bodies. Nevertheless, these somatic embryos were not exactly the same as their mature zygotic counterparts since differences were found in chloroplasts, amyloplasts, and nuclear structures. These observations suggest that additional factors might be required to obtain fully mature somatic embryos.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01379047
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  • 64
    Digitale Medien
    Digitale Medien
    Skeletal muscle damage during tourniquet-induced ischaemia (1993)
    Springer
    European journal of applied physiology 67 (1993), S. 342-347 
    Details
    ISSN: 1439-6327
    Schlagwort(e): Tourniquet ; Ischaemia ; Skeletal muscle ; Damage ; Lysosomes ; Oedema ; Capillaries ; Ultrastructure
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary Muscle biopsies from the vastus lateralis muscle of patients who had undergone anterior cruciate ligament surgery under conditions of tourniquet-induced ischaemia were examined under the electron microscope at different periods of time up to 90 min of ischaemia. The severity of the alterations in ultrastructure appeared to depend on the period of ischaemia. The pathological changes consisted of accumulation of lysosomes, persistent intrafibre oedema, and some extracellular oedema. Signs of fibre necrosis were found after 90 min of ischaemia. Capillary ultrastructure was only altered with regard to some swelling of the endothelium and marked thickening of the basement membrane. It was concluded that skeletal muscle could be severely affected even during relatively short periods of ischaemia, which might facilitate the development of muscle atrophy during immobilization after orthopaedic surgery.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00357633
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  • 65
    Digitale Medien
    Digitale Medien
    Genetic counseling and evaluation of recurrence for people with physical disabilities (1993)
    Springer
    Sexuality and disability 11 (1993), S. 221-228 
    Details
    ISSN: 1573-6717
    Schlagwort(e): Genetics ; disability ; reproduction
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin , Psychologie
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01102581
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  • 66
    Digitale Medien
    Digitale Medien
    Ultrastructural changes in salivary glands after different adrenergic and cholinergic stimulations (1993)
    Springer
    European archives of oto-rhino-laryngology and head & neck 250 (1993), S. 33-39 
    Details
    ISSN: 1434-4726
    Schlagwort(e): Rat salivary gland ; Ultrastructure ; Adrenergic stimulation ; Cholinergic stimulation ; Sialagogues
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary We have compared four different sialagogues and their degranulating effect on serous and mucous cells, and their long-term effects. From this and earlier experiments, even within the groups of α- and β-adrenergic agents used, the effects varied on the serous and mucous cells. Previous studies have shown that cyclocytidine effectively degranulates serous cells without signs of cellular damage, while carbachol predominantly affects mucous acinar cells but gives early rise to permanent gland damage. Noradrenaline affects both serous and mucous cells, predominantly affecting serous cells with initial mitochondrial damage. Clonidine partially depletes both serous and mucous cells of their granules, producing permanent cellular damage. One month after a single injection of cyclocytidine the early findings described had disappeared. Carbachol showed permanent damage to salivary gland parenchyma, and both noradrenaline and clonidine demonstrated a long-term effect on acinar mucinous cells.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00176946
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  • 67
    Digitale Medien
    Digitale Medien
    Ultrastructural observation on hepatocellular carcinoma (1993)
    Springer
    Medical molecular morphology 26 (1993), S. 19-28 
    Details
    ISSN: 1860-1499
    Schlagwort(e): Hepatocellular carcinoma ; Tumor grade ; Ultrastructure ; Morphometry ; Cell organelles
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract In 31 cases of hepatocellular carcinoma, electron microscopic observation and morphometry on the cell organelles were carried out to evaluate the usefulness of electron microscopy for the diagnosis of well differentiated hepatocellular carcinoma. The cell organelles in well differentiated tumor cells were very similar to those in normal hepatocytes or hepatocytes with liver cirrhosis (LC). We found that in poorly differentiated tumor cells, the nuclear area, N/C ratio, nucleolar area, the amount of dispersed chromatin, and the number of free ribosomes had increased, but the cellular area, degree of nuclear roundness, and mitochondrial area had decreased. These results seem to indicate that electron microscopy is not as useful as light microscopy in the diagnosis of well differentiated hepatocellular carcinoma, but is useful in the study of poorly differentiated tumor cells, which indicated that the cell proliferation through mitoses was activated.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02348832
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  • 68
    Digitale Medien
    Digitale Medien
    Ultrastructural effects of immobilization on rat muscle spindles (1993)
    Springer
    Medical molecular morphology 26 (1993), S. 65-75 
    Details
    ISSN: 1860-1499
    Schlagwort(e): Ultrastructure ; Rats ; Muscle spindles ; Immobilization
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract The ultrastructure of rat muscle spindles was examined after the anterior tibial muscles had been immobilized in a plaster cast. There was an increase in the number of collagen fibrils and external laminae around the outer capsules and in the intracapsular space 2 weeks after immobilization. The changes in the intrafusal muscle fibers within 4 weeks included disorientation of myofilaments. After 6 weeks, Z bands had become disarranged, and there was vacuolar degeneration of the sarcoplasmic reticulum in some fibers. Myelin sheaths of many of the myelinated nerve fibers (especially the thick ones, which were probably sensory nerve fibers) had degenerated within 2 weeks. These results indicate that immobilization of skeletal muscles affects not only extrafusal muscle fibers but also the structure of the muscle spindle.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02348837
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  • 69
    Digitale Medien
    Digitale Medien
    Scanning electron microscopic study of the adhesion of a human megakaryocytic leukemia cell line (CMK11-5) (1993)
    Springer
    Medical molecular morphology 26 (1993), S. 81-87 
    Details
    ISSN: 1860-1499
    Schlagwort(e): Human megakaryocytic leukemia cell line ; Adhesion ; Glycoprotein Ib ; Ultrastructure
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract To characterize the CMK11-5 subclone of a human megakaryocytic leukemia cell line (CMK), an ultrastructural study on adhesion was performed. The CMK11-5 cells showed irreversible attachment to rabbit aortic subendothelium accompanied by several morphological changes such as flattening and the spreading out of pseudopodia. These morphological changes are similar to those observed in normal platelets during attachment. The attachment of CMK11-5 cells to rabbit aortic subendothelium indicates that human megakaryocytes may possess an adhesion function like that of platelets. The CMK cell line and its CMK11-5 subclone with megakaryocytic properties appear to be useful for studying the function of megakaryocytes.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02348032
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  • 70
    Digitale Medien
    Digitale Medien
    Ultrastructural localization ofcalcium dependent adenosine triphosphatase (ATPase) in the myocardium of endotoxin-administered rats (1993)
    Springer
    Medical molecular morphology 26 (1993), S. 151-155 
    Details
    ISSN: 1860-1499
    Schlagwort(e): Endotoxemia ; Enzyme histochemistry ; Ca++-ATPase ; Ultrastructure ; Myocardium
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract Enzyme-cytochemical studies were performed to clarify the pathogenesis of myocardial damage in endotoxemia. The reaction product of Ca++-ATPase was localized on the pinocytotic vesicles and plasma membrane of endothelial cells of capillaries and sarcolemma, sarcoplasmic reticulum and mitochondria of cardiomyocytes in control rats. However, the enzyme reaction product was reduced in amount and irregularly distributed on the plasma membrane, and on the increased number of pinocytotic vesicles of the endothelial cells of capillaries and the sarcolemma and sarcoplasmic reticulum of edematous cardiomyocytes after endotoxin injection. It is concluded that endotoxin induces structural changes and decreased activity in Ca++-ATPase in the vascular endothelium and myocytes of the heart.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02348041
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  • 71
    Digitale Medien
    Digitale Medien
    Ultrastructure of experimental candidial lesions in the rabbit esophagus (1993)
    Springer
    Medical molecular morphology 26 (1993), S. 215-217 
    Details
    ISSN: 1860-1499
    Schlagwort(e): Ultrastructure ; Esophagus ; Candida albicans ; Candidial lesion
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract Although the esophagus is the most frequent site ofCandida infections in the gastrointestinal tract, and many clinical studies about it have been reported, little attention has been directed toward experimental candidiasis of the esophagus, especially with regard to its ultrastructure. Using transmission electron microscopy, this study was performed to clarify the ultrastructure of experimental lesions, obtained from five New Zealand white male rabbits which were given a suspension ofCandida albicans cells (107/ml) for 13 days. The results showed that the lesions consisted of exfoliating, squamous epithelial cells with mycelial elements ofCandida albicans cells penetrating through them, and that a widened intercellular space between individual cells in the area of candidial invasion seems to be a characteristic finding of candidial infection.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02348004
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  • 72
    Digitale Medien
    Digitale Medien
    Salmon roe-like amyloid deposition in a prolactinoma: a case report (1993)
    Springer
    Brain tumor pathology 20 (1993), S. 89-92 
    Details
    ISSN: 1861-387X
    Schlagwort(e): Amyloid ; Magnetic resonance imaging ; Pituitary adenoma ; Prolactinoma ; Ultrastructure
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract An unusual case of prolactin-producing adenoma with extensive amyloid deposition is reported to clarify its radiological, intraoperative, and light- and electron-microscopic findings. A 41-year-old female patient complained of amenorrhea persisting for 20 years. Magnetic resonance imaging (MRI) revealed a pituitary adenoma, which included low-intensity spots on T1- and T2-weighted images. Intraoperative examination found multiple small, yellowish, spherical masses resembling salmon roe within the adenoma. Light and electron microscopy revealed the presence of immunoreactive cells for prolactin intermingled with concentric lamellar bodies of radially arranged amyloid fibrils originating from the endoplasmic reticulum in prolactinoma cells. The extracellular lamellar amyloid deposits were apparently due to degradation of prolactin-producing cells, but the reason for the production and radially arranged accumulation of amyloid remains to be identified.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02483453
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  • 73
    Digitale Medien
    Digitale Medien
    A fourth ventricle atypical teratoid/rhabdoid tumor in an infant (1993)
    Springer
    Brain tumor pathology 20 (1993), S. 47-52 
    Details
    ISSN: 1861-387X
    Schlagwort(e): Atypical teratoid/rhabdoid tumor ; Fourth ventricle ; Immunohistochemistry ; Ultrastructure ; Basal lamina
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract Atypical teratoid/rhabdoid tumor (AT/RT), a recently established central nervous system tumor entity, occurs in children and is more malignant than medulloblastoma/primitive neuroectodermal tumors (PNET). We report here a case of AT/RT in a male infant who was 9 months old at the time of diagnosis. Magnetic resonance imaging revealed that the tumor occupied the fourth ventricle, and at surgery it was found to adhere to the floor of the fourth ventricle. After subtotal removal of the tumor mass, chemotherapy and radiotherapy were performed, but the patient died about 8 months after the diagnosis following rapid regrowth of the residual tumor. Light-microscopically, the tumor was composed mainly of nests of rhabdoid cells with fields of PNET. Occasional mesenchymal and epithelial fields were also evident. Immunohistochemically, these rhabdoid cells were positive for vimentin, epithelial membrane antigen, smooth-muscle actin, cytokeratin, and S-100 protein, and less frequently for glial fibrillary acidic protein. Electron-microscopically, the typical rhabdoid cells contained whorled bundles of intermediate filaments in their cytoplasm. Occasionally, such rhabdoid cells were covered partially by basal lamina at their stromal interface. These findings are typical of AT/RT. Although it is well known that AT/RT often arises in the posterior fossa, detailed reports of cases affecting the fourth ventricle are rare. In this case, the ultrastructural relationship between rhabdoid cells and the basal lamina, which has not so far been described in AT/RT, was of great interest when the nature of the rhabdoid cells was considered.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02483446
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  • 74
    Digitale Medien
    Digitale Medien
    Non-invasive genotyping of primates (1993)
    Springer
    Primates 34 (1993), S. 333-346 
    Details
    ISSN: 0032-8332
    Schlagwort(e): Genetics ; Pedigrees ; Molecular evolution ; Pan ; Hylobates ; Macaca ; DNA sequences ; Microsatellite loci
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Using DNA amplified from shed or plucked hair follicles it is now possible to genotype individual primates at many nuclear and mitochondrial gene loci. Sequence specific primers and the polymerase chain reaction permit the rapid production of sufficient DNA from a single hair for numerous analyses. The direct sequencing of relatively conservative mtDNA sequences like cytochromeb is proving useful in establishing species and subspecies-level relationships. More variable sequences (e.g. the mtDNA control region or D-loop) are useful at the population and social community levels. Paternity exclusion, pedigree relationships, and community structure can be determined using simple sequence length polymorphisms (SSLPs) of multiple hypervariable nuclear microsatellite or simple sequence repeat (SSR) loci. Studies involving captive and free-ranging chimpanzees, gibbons, and macaques illustrate the resolving power of these new non-invasive molecular genetic genotyping techniques.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02382629
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  • 75
    Digitale Medien
    Digitale Medien
    Immunocytochemistry of ambiguous cells in adult and embryonic dwarf (dw) mouse pituitaries (1993)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 236 (1993), S. 671-678 
    Details
    ISSN: 0003-276X
    Schlagwort(e): Dwarf Mouse ; Pituitary Gland ; Embryo ; Immunocytochemistry ; Ultrastructure ; Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Medizin
    Notizen: In the pars distalis of the pituitary gland in adult and embryonic dwarf (dw/dw) mutant mice, ambiguous cells exhibiting ultrastructural features common to growth hormone (GH) cells and prolactin (Prl) cells were analyzed by means of colloidal gold ultrastructural immunocytochemistry in order to define the functional nature of these peculiar cells. Adult and 18-day embryonic pituitaries from normal (+/+; dw/+) and dwarf (dw/dw) mice were processed with antibodies to GH, Prl, TSH (thyroid-stimulating hormone), ACTH (adrenocorticotropic hormone), LH (luteinizing hormone), FSH (follicle-stimulating hormone), and HCG (chorionic gonadotropic hormone). In the adult and embryonic dwarf pituitaries, the ambiguous cells reacted negatively to all of the antibodies except for anti-ACTH, which labeled them well. In addition, the ACTH-positive cells showed a much wider variety of shapes and granule size and distribution, as compared with normal adults. In the embryos, this variability in ACTH cell morphology occurred not only in dwarf embryos, but in their normal counterparts as well. The results thus suggest that adult dwarf pituitaries may retain an embryonic or incompletely differentiated form of ACTH cells. © 1993 Wiley-Liss, Inc.
    Zusätzliches Material: 9 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/ar.1092360411
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  • 76
    Digitale Medien
    Digitale Medien
    Ultrastructure of the human anogenital “sweat” gland (1993)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 235 (1993), S. 583-590 
    Details
    ISSN: 0003-276X
    Schlagwort(e): Skin ; Vulva ; Sweat glands ; Histology ; Ultrastructure ; Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Medizin
    Notizen: A newly described type of cutaneous gland occurring in the human anogenital region was investigated in specimens from the vulva by electron microscopy. This gland, which is characterized by a long excretory duct opening at the skin surface, by a wide coiled secretory part with multiple lateral extensions in the form of diverticula and branches lined by a two-layered pseudostratified of myoepithelium, and by a luminal layer of tall columnar cells with conspicuous “snouts”, could not be categorized as an eccrine, apocrine, or mammary gland. Electron microscopy confirmed its separate position by showing that the luminal layer of secretory cells with prominent cytoplasmic caps had elaborately folded lateral membranes, occasional canaliculi, and a large number of uniform electron-lucent to moderately electron-dense secretory granules as part of a probable merocrine secretion. The excetory duct showed a poorly developed cuticular border. This combination of ultra-structural features is alien to the other tubular cutaneous glands. The function of this anogenital “sweat” gland remains obscure, but the presence of these granules suggests a secretion product that is different from that of other cutaneous glands. © 1993 Wiley-Liss, Inc.
    Zusätzliches Material: 5 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/ar.1092350411
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  • 77
    Digitale Medien
    Digitale Medien
    Chronology of peroxidase activity in the developing rat parotid gland (1993)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 235 (1993), S. 611-621 
    Details
    ISSN: 0003-276X
    Schlagwort(e): Peroxidase ; Parotid gland ; Salivary gland ; Rat ; Growth and development ; Biochemistry ; Cytochemistry ; Ultrastructure ; Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Medizin
    Notizen: The course of development of salivary peroxidase, an enzyme that has an important role in oral defense mechanisms, has been well documented in rat submandibular glands. However, the only report on salivary peroxidase activity in the other major salivary glands of the rat has been a cytochemical study of the adult parotid gland. In the present investigation, the accumulation of salivary peroxidase activity in developing parotid glands of rats was followed both biochemically and cytochemically. Specific activity (units per mg protein) attributable to salivary peroxidase began at 1 day after birth, then rose rapidly but unevenly, with peaks at 21 and 70 days, and no difference between the sexes at any age. Activity per gland increased progressively to 42 days in both sexes and was significantly higher in males at 70 days. The cytochemical observations on peroxidase activity localized to the rough endoplasmic reticulum and secretory granules of the developing acini were well correlated with the biochemical findings. Peroxidase-negative cells occurred in immature acini at 1 and 7 days, but only in the intercalated ducts thereafter. This observation suggests that the acini are a source of some of the ductal cells, at least during early postnatal development. The developmental pattern of specific activity differed from those of other rat parotid secretory enzymes, indicating that control of their synthesis during development is noncoordinate. The patterns of specific activity of the parotid and submandibular glands were complementary, suggesting that their combined secretions may supply biologically significant peroxidase activity to the oral cavities of rats throughout postnatal development. © 1993 Wiley-Liss, Inc.
    Zusätzliches Material: 13 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/ar.1092350414
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  • 78
    Digitale Medien
    Digitale Medien
    Ultrastructure of lipid-containing cells of the anterior pituitary gland of the domestic chicken, Gallus domesticus (1993)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 237 (1993), S. 506-511 
    Details
    ISSN: 0003-276X
    Schlagwort(e): Anterior pituitary ; Domestic chicken ; Lipid cells ; Ultrastructure ; Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Medizin
    Notizen: A combination of light and electron microscopy techniques was applied to characterize a luteinizing hormone (LH)-gonadotroph-like cell in the anterior pituitary gland of the adult domestic chicken. This cell type (mean ± sem, 91 ± 8 μm2) was larger than typical LH-gonadotrophs (71 ± 5 μm2, P 〈 0.01) and seen in pituitary glands from adult males but not those from adult females of the same age. The ultrastructural features of these cells were similar to typical LH-gnoadotrophs in the same section, except for the presence of large (1,126 ± 77 nm diameter), round, or polymorphic electron-dense inclusions (14 ± 3 per cell) in the cisternae of the endoplasmic reticulum. When resin sections of anterior pituitary gland were stained with the vapours from the fixative osmium tetroxide and a solution of potassium ferricyanide, inclusions were found that appeared to be lipid in nature and were circumscribed by the cisternal membranes of the endoplasmic reticulum. These lipid inclusions were associated occasionally with acid phosphatase and lysosome-like bodies. The cellular pigment, lipofuscin, considered to be a marker of aging, was absent from the anterior pituitary glands from these adult chickens. These observations suggest that the appearance of lipid in LH-gonadotrophs is unlikely to be age-related and an alternative explanation is discussed in relation to a sex difference in the LH response of adult chickens to gonadotrophin-releasing hormone (GnRH). © 1993 Wiley-Liss, Inc.
    Zusätzliches Material: 4 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/ar.1092370410
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  • 79
    Digitale Medien
    Digitale Medien
    Ultrastructural characteristics of primordial germ cells in the quail embryo (1993)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 236 (1993), S. 547-552 
    Details
    ISSN: 0003-276X
    Schlagwort(e): Primordial germ cells ; Ultrastructure ; Nucleolus ; Quail embryo ; Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Medizin
    Notizen: An avian species, the quail has become a desirable animal model in experimental embryology and reproductive biology. To understand the ultrastructural characteristics of primordial germ cells (PGC) of this species, we studied PGC in the Japanese quail (Coturnix coturnix japonica) embryo at various developmental stages from their appearance in the germinal crescent through migration to settlement in the gonadal ridges by means of electron microscopy. The results were compared with those of another well-known avian species, the chick. Several ultrastructural characteristics of quail PGC not described previously in chick PGC were observed as follows: (1) No glycogen particles were detected in the cytoplasm at any stage examined. (2) Electron-dense and membrane-bounded granules were found in the PGC cytoplasm during the sexually indifferent gonadal stages. (3) Quail PGC were characterized by a prominent nucleolus associated with condensed chromatin (heterochromatin), and the developmental changes of the nucleus, were noted; the nucleolus initially appeared as a compact mass at the germinal crescent stage and became dispersed at later stages during the colonization of the gonadal ridges. These findings suggest several physiological and functional differences in the cell cycle between these two avian species. This is the first report describing detailed ultrastructural characteristics of PGC in the quail embryo. © 1993 Wiley-Liss, Inc.
    Zusätzliches Material: 11 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/ar.1092360314
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  • 80
    Digitale Medien
    Digitale Medien
    Cytologic structures unique to deiters cells of the cochlea (1993)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 237 (1993), S. 421-430 
    Details
    ISSN: 0003-276X
    Schlagwort(e): Organ of Corti ; Ultrastructure ; Cytoskeleton ; Gerbil ; Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Medizin
    Notizen: Deiters cells in the gerbil cochlea disclosed unusual ultrastructural features. A sharp transition zone separated the cell body underlying outer hair cells into an upper compartment with numerous organelles and a lower part devoid of structures other than the microtubule stalk. Deiters cells exhibited a unique structure, the rosette complex, which consisted of a core of densely fibrillar trabeculae, enclosed in a filamentous meshwork and surrounded by tubulocisternal endoplasmic reticulum. The dense trabeculae radiated in columns downward from an apical translucent area toward a lucent zone around the nucleus. They also spread to the medial plasmalemma enveloping nerves and upward into the base of the phalanx. Frequent, small Golgi complexes bordered the tubular reticulum. The distinctive mitochondria of Deiters cells frequently paralleled the plasmalemma, revealed an elongated, often arched profile, and contained sparse, longitudinally aligned cristae. The stalk, composed of characteristic microtubule bundles resembling those in pillar cells, ascended from basal to apical plasmalemma of the cell body and into the phalanx and reticular lamina as previously described. The stalk appeared also to ramify into smaller microtubule bundles in apical cytosol penetrating the rosette complex. Nuclei in Deiters cells differed from those in hair cells in their location high in the cell and in showing chromatin dispersion indicative of more active protein synthesis. © 1993 Wiley-Liss, Inc.
    Zusätzliches Material: 18 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/ar.1092370316
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  • 81
    Digitale Medien
    Digitale Medien
    Ultrastructural changes in the efferent duct and epididymis of men with obstructive infertility (1993)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 237 (1993), S. 199-207 
    Details
    ISSN: 0003-276X
    Schlagwort(e): Epididymis ; Ultrastructure ; Obstructive infertility ; Vasoepididymostomy ; Spermatozoa ; Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Medizin
    Notizen: Ultrastructural changes in the efferent duct and in different regions of the epididymis in men with obstructive azoospermia were compared with corresponding tissues collected from men of proven fertility who underwent castration due to malignancy of the prostate. Major degenerative changes were seen in the efferent duct and the caput epididymidis of men with obstruction at the caput epididymidis which may have been induced by fluid pressure due to defective absorption of testicular fluid in the caput epididymidis. These degenerative changes included decrease in tubular and lumen diameter of the caput and the cauda epididymides, decrease in height of the stereocilia, reduction in-rough endoplasmic reticulum and Golgi material, and presence of lipofuscin and osmiophilic dense bodies. The degenerative changes were less when the site of obstruction was in the cauda epididymidis since fluid reabsorption would continue to take place normally in the caput epididymidis. In men who had undergone vasoepididymostomy (VEA), the ejaculated spermatozoa showed a high percentage of morphological abnormalities which may have occurred due to adverse effects of long-term obstruction on spermatogenesis. © 1993 Wiley-Liss Inc.
    Zusätzliches Material: 19 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/ar.1092370207
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  • 82
    Digitale Medien
    Digitale Medien
    Posterior pituitary of the newborn marsupial possum, Trichosurus vulpecula (1993)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 237 (1993), S. 228-235 
    Details
    ISSN: 0003-276X
    Schlagwort(e): Posterior pituitary ; Marsupial ; Trichosurus vulpecula newborn ; Ultrastructure ; Immunocytochemistry ; Mestocin ; Vasopressin ; Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Medizin
    Notizen: The fetal anterior pituitary-adrenal axis is thought to be involved in the initiation of birth in both eutherian and marsupial mammals. Little is known about the structure and function of the posterior pituitary at birth in the marsupial. Immunocytochemistry, high pressure liquid chromatography, and radioimmunoassay were used to identify vasopressin and mesotocin in the posterior pituitary of a newborn marsupial, the brushtail possum, Trichosurus vulpecula. The concentrations of vasopressin and mesotocin in the head of the newborn possum were 0.34 and 0.28 ng, respectively. The concentration of vasopressin was always greater than that of mesotocin, and the amounts of neuropeptides present in the head increased as the possum developed. © 1993 Wiley-Liss Inc.
    Zusätzliches Material: 9 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/ar.1092370210
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  • 83
    Digitale Medien
    Digitale Medien
    Immunoelectron microscope localization of snRNP, hnRNP, and ribosomal proteins in mouse spermatogenesis (1993)
    New York, NY [u.a.] : Wiley-Blackwell
    Molecular Reproduction and Development 35 (1993), S. 261-271 
    Details
    ISSN: 1040-452X
    Schlagwort(e): Immunocytochemistry ; Ultrastructure ; Perichromatin granules ; Interchromatin granules ; Mouse spermatids ; Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie
    Notizen: We have studied the ultrastructural distribution of heterogeneous nuclear ribonucleoproteins (hnRNPs), small nuclear ribonucleoproteins (snRNPs), and ribosomal proteins during mouse spermatogenesis and spermiogenesis by means of specific antibodies and immunocytochemistry.All the above components were detectable from primary spermatocytes until the spermatid elongation phase, when the RNA synthetic activity is known to cease. Ribosomal protein (P1/P2 and L7) labeling disappeared as early as during the acrosome phase, and nucleoli were no longer labeled even during the cap phase. The nucleoplasmic structures labeled with the different anti-nucleoplasmic RNP immunoprobes corresponded, until the acrosome phase, to those previously observed as targets of the same antibodies in the nucleoplasm of somatic cell nuclei. Clusters of interchromatin granules of spermatocyte and early spermatid nuclei exhibit some labeling for hnRNP when compared with nuclei of Sertoli cells or previously analyzed liver or tissue culture cells, where these structural constituents usually remain weakly labeled or unlabeled.In spermatids in step 10, another type of nuclear granule, resembling perichromatin granules, but occurring in aggregates, can be observed. These structural constituents were labeled with antibodies recognizing nucleoplasmic snRNP antigens and therefore suggesting a non-nucleolar origin of these granules.Finally, we have observed nucleoplasmic areas of fibrogranular material, occurring only in primary spermatocytes. These components were labeled with anti-ribosomal protein antibodies but did not contain either hnRNPs or snRNPs. © 1993 Wiley-Liss, Inc.
    Zusätzliches Material: 15 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/mrd.1080350308
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  • 84
    Digitale Medien
    Digitale Medien
    Comparison of hexamethyldisilazane (HMDS), Peldri II, and critical-point drying methods for scanning electron microscopy of biological specimens (1993)
    New York, NY [u.a.] : Wiley-Blackwell
    Microscopy Research and Technique 26 (1993), S. 489-495 
    Details
    ISSN: 1059-910X
    Schlagwort(e): Ultrastructure ; Tissue preparation ; Animal ; Plant ; Leaf ; Cuticle ; Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Allgemeine Naturwissenschaft
    Notizen: Three different drying methods, critical-point drying (CPD), Peldri II, and hexamethyldisilazane (HMDS), were compared using representative animal( rat kidney, trachea, duodenum, lung, and red blood cells) and plant( leaves from ten species of monocotyledons and dicotyledons) specimens. All three drying methods produced identical results with animal specimens. Plant specimens showed signs of shrinkage regardless of which drying method was employed. The order of preservation quality from best to worst for leaves was CPD 〉 Peldri II 〉 HMDS, with the CPD method providing substantially better results in all but one case. Postfixation of leaves with osmium tetroxide resulted in poorer preservation in all instances. Peldri II caused complete extraction of leaf cuticular wax, while both both CPD and HMDS showed minimal extraction compared with samples air dried directly from acetone. These results indicate that HMDS provides a time-saving and inexpensive alternative to CPD for animal specimens. Plant specimens, particularly those containing cells with large central vacuoles, are adequately preserved only with the CPD method. In addition, postfixation with osmium should be avoided when processing plant specimens for scanning electron microscopy. © 1993 Wiley-Liss, Inc.
    Zusätzliches Material: 5 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/jemt.1070260603
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  • 85
    Digitale Medien
    Digitale Medien
    Electron microscopic immunocytochemistry of glutamate-containing nerve fibers in the taste bud of mudpuppy (Necturus maculosus) (1993)
    New York, NY [u.a.] : Wiley-Blackwell
    Microscopy Research and Technique 26 (1993), S. 225-230 
    Details
    ISSN: 1059-910X
    Schlagwort(e): Gustation ; Neurotransmitters ; Ultrastructure ; Amphibia ; Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Allgemeine Naturwissenschaft
    Notizen: The presence of glutamate immunoreactivity (glu-IR) in the nerve fibers of the mudpuppy taste bud was investigated by electron microscopy. Pre-embedding staining with avidinbiotin-peroxidase complex (ABC) and post-embedding staining with 5 mm colloid gold conjugates were used separately to identify immuno-stained structures. We have found the following: 1) the majority of the nerve fibers innervating the mudpuppy taste bud are unmyelinated; 2) about 85% of nerve fibers located at the base of the taste bud and about 60% of the nerve fibers located between the taste cells show glu-IR by pre-embedding staining; 3) there is a preferential staining of the glu-IR in the nerve fibers of the mudpuppy taste bud; and 4) the distribution of the colloidal gold particles in the nerve fibers is 1.5 to 2 times denser than that of the staining in the connective tissue background or cellular profiles of taste cells. From the distribution and pattern of the nerve fibers obtained in the thick and thin sections, we conclude that the mudpuppy taste bud is innervated by glutamate-containing unmyelinated nerve fibers. © 1993 Wiley-Liss, Inc.
    Zusätzliches Material: 8 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/jemt.1070260305
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  • 86
    Digitale Medien
    Digitale Medien
    Process of nuclear envelope reduction in spermiogenesis of a mosquito, Culex tigripes (1993)
    New York, NY [u.a.] : Wiley-Blackwell
    Molecular Reproduction and Development 34 (1993), S. 416-419 
    Details
    ISSN: 1040-452X
    Schlagwort(e): Spermatid ; Ultrastructure ; Diptera ; Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie
    Notizen: When the Culex tigripes spermatid begins to elongate, the nucleus exhibits on its surface invaginations of the nuclear envelope. These invaginations have a uniform diameter of 0.3 μm. They separate from the envelope of the nucleus and form spherical intranuclear vesicles. In the old spermatids these vesicles are imprisoned in the condensed chromatin. The spermatozoon also possesses these vesicles which are then ovoid in shape. This process of vesiculation permits the diminution of the surface of the nucleus when it decreases in volume during spermiogenesis. © 1993 Wiley-Liss, Inc.
    Zusätzliches Material: 9 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/mrd.1080340410
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  • 87
    Digitale Medien
    Digitale Medien
    Electron microscopy of intracellularly labeled neurons in the hippocampal slice preparation (1993)
    New York, NY [u.a.] : Wiley-Blackwell
    Microscopy Research and Technique 24 (1993), S. 67-84 
    Details
    ISSN: 1059-910X
    Schlagwort(e): Intracellular labeling ; Horseradish peroxidase ; Lucifer Yellow ; Biocytin ; Neurobiotin ; Ultrastructure ; Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Allgemeine Naturwissenschaft
    Notizen: We have assessed the properties of three intracellular markers, horseradish peroxidase, biocytin/Neurobiotin, and Lucifer Yellow, and have compared their usefulness as neuronal markers for light and electron microscopic visualization. Neurons in the acute slice preparation of rat hippocampus were filled with one of these markers, and the marker was converted to an optical and electron-dense reaction product. Dimethylsulfoxide (DMSO) greatly facilitated penetration of recognition reagents while preserving membrane integrity. The markers were compared with respect to injection parameters, mobility and recognition, stability and visibility, and ultrastructural clarity. Horseradish peroxidase (HRP)-labeled neurons, recognized histochemically with diaminobenzedine (DAB), were easily visualized by the density of the DAB reaction product; however, the electron density was often so great as to obscure ultrastructural details. Biocytin (BC)-/Neurobiotin (NB)-labeled neurons were recognized by avidin-HRP, followed by histochemical localization of HRP with DAB. The optically dense reaction product gave complete visualization of the soma and processes at the light microscopic level. The electron density was homogeneously distributed throughout the cell, so that ultrastructural features were easily identified. Lucifer Yellow (LY), a fluorescent marker, was converted to an optical and electron-dense reaction product via immunocytochemical staining with a rabbit anti-LY antibody, followed by goat anti-rabbit IgG-HRP and DAB histochemical localization. Similar to BC/NB, the reaction product was evenly dispersed, providing good light microscopic and ultrastructural clarity. Under our experimental conditions, BC/NB and LY were superior markers that could be used routinely to label neurons, and give excellent visualization not only at the light but also at the electron microscopic level. © 1993 Wiley-Liss, Inc.
    Zusätzliches Material: 32 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/jemt.1070240107
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  • 88
    Digitale Medien
    Digitale Medien
    Characteristics of uterine aging (1993)
    New York, NY [u.a.] : Wiley-Blackwell
    Microscopy Research and Technique 25 (1993), S. 148-168 
    Details
    ISSN: 1059-910X
    Schlagwort(e): Uterus ; Endometrium ; Decidua ; Collagen ; Ultrastructure ; Rodent ; Human ; Review ; Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Allgemeine Naturwissenschaft
    Notizen: Uterine aging is in part responsible for a decline in fecundity which begins in middle age in most mammals. Characteristics of uteri from a variety of animals in middle age and old age are reviewed and the factors which may be responsible for this decline discussed. These include age-related changes in the hypothalamus, pituitary, and ovaries; loss of number or function of steroid hormone receptors; morphological changes in the uterine epithelium; the accumulation of collagen fibrils in the uterine stroma; and loss or impairment of the decidual response. The ultra-structural morphology of uterine epithelial, stromal, and decidual tissue from 4 month old and 18 month old Fischer 344 rats is presented and compared. © 1993 Wiley-Liss, Inc.
    Zusätzliches Material: 23 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/jemt.1070250207
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  • 89
    Digitale Medien
    Digitale Medien
    Cellular relations in mouse circumvallate taste buds (1993)
    New York, NY [u.a.] : Wiley-Blackwell
    Microscopy Research and Technique 26 (1993), S. 209-224 
    Details
    ISSN: 1059-910X
    Schlagwort(e): Cell types ; Ultrastructure ; Mammalian ; Ninth nerve ; Dense-cored vesicles ; Papilla ; Rabbit ; Trophic ; Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Allgemeine Naturwissenschaft
    Notizen: The fine structure of the taste buds of circumvallate papillae of two strains of mice was studied by electron microscopy. Mice anesthetized with ketamine were perfused through the heart with a double aldehyde mixture in cacodylate buffer and the tissues embedded in Epon. Semi-serial sections were employed. The morphology and relationships of cell types are consistent with the majority of descriptions of mammalian taste buds served by the ninth cranial nerve. Cells of type II are particularly well documented, as the stages in their origin, maturation and degeneration could be followed. Significant differences, however, relate to cell type I. These cells contain large dense-cored granules, contrasted with the more irregular and somewhat larger dark granules of the type I cells in the rabbit. These granules do not produce a dense homogenous product for the pore, as seen in the rabbit. Rather the pore substance consists of small, empty vesicles in a diffuse dark matrix. These granules are only moderately larger than the dense-cored vesicles of the type III cells. All features of the type III cell were demonstrated, although no instance of a complete cell was seen in any section. No significant differences were noted between the two strains of mice. Intimate proximity of a nerve to a cell nucleolus, suggestive of a trophic pathway, is illustrated. © 1993 Wiley-Liss, Inc.
    Zusätzliches Material: 13 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/jemt.1070260304
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  • 90
    Digitale Medien
    Digitale Medien
    The independent stage-specific expression of the 18-kDa heat shock protein genes during microsporogenesis in Zea mays L (1993)
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 14 (1993), S. 15-26 
    Details
    ISSN: 0192-253X
    Schlagwort(e): Heat shock protein ; maize ; mi-crosporogenesis ; gametogenesis ; Life and Medical Sciences ; Genetics
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie
    Notizen: The small (18-kDa) heat shock proteins (hsps) of maize are encoded by a complex multigene family. In a previous report, we described the genetic information from cDNAs encoding two different members of the family. In this communication, we report the isolation and characterization of cDNA and genomic clones encoding information for a third member of this hsp family (c/gMHSP18-1). DNA fragments containing nucleotide sequences common to, or specific for, each of these characterized 18-kDa genes were prepared and used as probes to assess the expression of these genes during microsporogenesis and development of the gametophyte in an inbred line of maize (Oh43). Our results demonstrate (1) that mRNA transcripts encoding the 18-kDa hsps are expressed and/or accumulate during microsporogenesis, and (2) that genes encoding two of the characterized 18-kDa hsps are expressed and/or accumulate independently, in a stage-specific manner during microsporogenesis. These observations imply that the stage-specific expression of particular 18-kDa hsp genes results from gene-specific regulation during microsporogenesis and gametophyte development rather than from an overall activation of the heat shock or stress response. © 1993Wiley-Liss, Inc.
    Zusätzliches Material: 7 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/dvg.1020140104
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  • 91
    Digitale Medien
    Digitale Medien
    Expression of endogenous and microinjected hsp 30 genes in early Xenopus laevis embryos (1993)
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 14 (1993), S. 42-50 
    Details
    ISSN: 0192-253X
    Schlagwort(e): Development ; transcnption ; heat shock protein ; microinjection ; polymerase chain reaction ; Xenopus laevis ; mRNA ; Life and Medical Sciences ; Genetics
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie
    Notizen: In the present study, we have examined the regulation of expression of a newly isolated member of the hsp 30 gene family, hsp 30C. Using RT-PCR, we found that this gene was first heat-inducible at the tailbud stage of development. We also examined the expression of two microinjected modified hsp 30C gene constructs in Xenopus embryos. One of the constructs had 404 bp of hsp 30C 5′-flanking region, whereas the other had 3.6 kb. Both gene constructs had 1 kb of 3′-flanking region. RT-PCR assays were employed to detect the expression of these microinjected genes. The presence of extensive 5′- and 3′-flanking regions of the hsp 30C gene did not confer proper developmental regulation, since heat-inducible expression of both of the microinjected constructs was detectable at the midblastula stage. The premature expression of the microinjected hsp 30 gene was not a result of high plasmid copy number or the presence of plasmid DNA sequences. These results suggest that the microinjected genes contain all the cis-acting DNA sequences required for correct heat-inducible regulation but do not contain the elements required for the proper regulation of hsp 30 gene expression during development. It is possible that regulatory elements controlling the developmental expression of the hsp30 genes may reside upstream or downstream of the entire cluster. © 1993Wiley-Liss, Inc.
    Zusätzliches Material: 7 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/dvg.1020140106
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  • 92
    Digitale Medien
    Digitale Medien
    Regulation of two different hsp70 transcript populations in steroid hormone-induced fungal development (1993)
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 14 (1993), S. 6-14 
    Details
    ISSN: 0192-253X
    Schlagwort(e): hsp70 ; heat shock ; fungus ; steroid hormone ; secretion ; mycelial branching ; sexual differentiation ; Life and Medical Sciences ; Genetics
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie
    Notizen: In the filamentous oomycete fungus Achlya, the differentiation of gamete bearing structures on vegetative hyphae of the male mating type, is induced by the Achlya steroid hormone, antheridiol. Among the several metabolically labeled intracellular proteins whose synthesis or accumulation is altered by hormone treatment are steroid-induced 85-kDa and 68- to 78-kDa proteins. The 85-kDa protein was previously shown to be the Achlya heat shock protein hsp85 [Brunt et al., 1990; Brunt and Silver, 1991], a component of the putative Achlya steroid hormone receptor. It was of interest to determine if the antheridiol-induced “70-kDa” proteins were hsp70-family heat shock proteins and if hormone treatment-induced changes in the level of hsp70 transcripts. Two different Achlya hsp70 genomic sequences were cloned and used to investigate these questions. The two hsp70 sequences recognized two different mycelial transcript populations, one of which was regulated also by decreased glucose. Of note, both of the two hsp70 transcript populations were found to be regulated by antheridiol. The hormone-induced chcnges in hsp70 transcript levels were temporally correlated with the onset of massive lateral hyphal branching and alterations in the pattern of secreted N-linked glycoproteins which occur in hormone-treated mycelia. To our kncwledge, this represents one of the first reports on changes in hsp70 proteins and transcripts during fungal differentiation. Our results may have implications for the role of heat shock proteins in hyphal branching and secretion in filamentous fungi and perhaps other cell types. © 1993Wiley-Liss, Inc. Inc.
    Zusätzliches Material: 3 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/dvg.1020140103
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  • 93
    Digitale Medien
    Digitale Medien
    Characterization of two Maize HSP90 heat shock protein genes: Expression during heat shock, embryogenesis, and pollen development (1993)
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 14 (1993), S. 27-41 
    Details
    ISSN: 0192-253X
    Schlagwort(e): Heat shock inducible promoters ; hsp90 ; Zea mays ; developmental expression ; Life and Medical Sciences ; Genetics
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie
    Notizen: We have isolated two genes from Zea mays encoding proteins of 82 and 81 kD that are highly homologous to the Drosophila 83-kD heat shock protein gene and have analyzed the structure and pattern of expression of these two genes during heat shock and development. Southern blot analysis and hybrid select translations indicate that the highly homologous hsp82 and hsp81 genes are members of a small multigene family composed of at least two and perhaps three or more gene family members. The deduced amino acid sequence of these proteins based on the nucleotide sequence of the coding regions shows 64-88% amino acid homology to other hsp90 family genes from human, yeast, Drosophila, and Arabidopsis. The promoter regions of both the hsp82 and hsp81 genes contain several heat shock elements (HSEs), which are putative binding sites for heat shock transcription factor (HSF) commonly found in the promoters of other heat shock genes. Gene-specific oligonucleotide probes were synthesized and used to examine the mRNA expression patterns of the hsp81 and hsp82 genes during heat shock, embryogenesis, and pollen development. The hsp81 gene is only mildly heat inducible in leaf tissue, but is strongly expressed in the absence of heat shock during the premeiotic and meiotic prophase stages of pollen development and in embryos, as well as in heat-shocked embryos and tassels. The hsp82 gene shows strong heat inducibility at heat-shock temperatures (37-42°C) and in heat shocked embryos and tassels but is only weakly expressed in the absence of heat shock. Promoter-GUS reporter gene fusions made and analyzed by transient expression assays in Black Mexican Sweet (BMS) Maize protoplasts also indicate that the hsp82 and hsp81 are regulated differentially. The hsp82 promoter confers strong heat-inducible expression of the GUS reporter gene in heat-treated cells (60- to 80-fold over control levels), whereas the hsp81 promoter is only weakly heat inducible (5- to 10-fold over control levels). © 1993Wiley-Liss, Inc.
    Zusätzliches Material: 9 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/dvg.1020140105
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  • 94
    Digitale Medien
    Digitale Medien
    Ferritin gene expression is developmentally regulated and induced by heat shock in sea urchin embryos (1993)
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 14 (1993), S. 58-68 
    Details
    ISSN: 0192-253X
    Schlagwort(e): Ferritin ; heat shock ; development ; sea urchin ; Life and Medical Sciences ; Genetics
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie
    Notizen: A 20-kD protein identified as a subunit of the iron-binding protein ferritin is present in S. purpuratus and L. pictus sea urchin embryos. The synthesis of the protein is stimulated by an elevation in temperature or by an increase in iron supply. The developmental expression of this protein and its regulation during normal development and upon heat shock was investigated. In L. pictus, ferritin is present in the unfertilized egg and, as determined by Western blot analysis, its concentration remains approximately constant after fertilization up to the gastrulc-pluteus stage; there is a small transient decrease in the level of the protein in the early blastula at a time coinciding with the first clear indication of its de novo synthesis. Northern blots reveal no cytoplasmic ferritin transcripts in the unfertilized egg, but there occurs a dramatic increase in the RNA level from the late morulaearly blastula stage (12-14 hr) to the mesenchyme blastula-early gastrula (25-30 hr) stage. This developmentally regulated increase in the constitutive concentration of ferritin RNA is correlatable with the normal onset of synthesis of the protein. The overall degree and nature of induction of ferritin by heat is dependent on the developmental stage: at 10-16 hr postfertilization heat shock elicits an increase in both the concentration of RNA and the synthesis of the protein; in hatched blastula (18 hr) and in later embryos heat shock increases ferritin synthesis, without a corresponding increase in the mRNA level. It appears that different mechanisms operate in the developing sea urchin embryo to regulate the expression of ferritin during normal development and on exposure to heat stress, one dependent on the concentration of ferritin transcripts and another operating at the level of translational control. © 1993Wiley-Liss, Inc.
    Zusätzliches Material: 7 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/dvg.1020140108
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  • 95
    Digitale Medien
    Digitale Medien
    Heat shock gene expression and development. II. An overview of mammalian and avian developmental systems (1993)
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 14 (1993), S. 87-91 
    Details
    ISSN: 0192-253X
    Schlagwort(e): Heat shock ; translation ; transcription ; development ; mRNA ; differentiation ; mammals ; birds ; Life and Medical Sciences ; Genetics
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/dvg.1020140202
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  • 96
    Digitale Medien
    Digitale Medien
    Development and tissue-specific distribution of mouse small heat shock protein hsp25 (1993)
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 14 (1993), S. 103-111 
    Details
    ISSN: 0192-253X
    Schlagwort(e): Mouse ; development ; small heat shock protein ; Life and Medical Sciences ; Genetics
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie
    Notizen: We have investigated the developmental and tissue-specific distribution of the mouse small hsp25 by immunohistology using an antibody that specifically identifies hsp25. Our analysis shows that the relative amount of hsp25 increases during embryogenesis. Through days 13-20 of embryogenesis, hsp25 accumulation is predominant in the various muscle tissues, including the heart, the bladder, and the back muscles. hsp25 is detectable also in neurons of the spinal cord and the purkinje cells. Furthermore analysis of the closely related α, B-crystallin shows that in several tissues, including the bladder, the notochordal sheath and the eye lens both proteins are coexpressed. Our studies demonstrate that mammalian hsp25 accumulation is developmentally regulated during mouse embryogenesis and support the view of an important functional role of small heat shock proteins in normal cell metabolism. © 1993Wiley-Liss, Inc.
    Zusätzliches Material: 6 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/dvg.1020140204
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  • 97
    Digitale Medien
    Digitale Medien
    HSP86 and HSP84 exhibit cellular specificity of expression and co-precipitate with an HSP70 family member in the murine testis (1993)
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 14 (1993), S. 119-126 
    Details
    ISSN: 0192-253X
    Schlagwort(e): Spermatogenesis ; HSP90 proteins ; HSP70 proteins ; Life and Medical Sciences ; Genetics
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie
    Notizen: This study extends to the protein level our previous observations, which had established the stage and cellular specificity of expression of hsp86 and hsp84 in the murine testis in the absence of exogenous stress. Immunoblot analysis was used to demonstrate that HSP86 protein was present throughout testicular development and that its levels increased with the appearance of differentiating germ cells. HSP86 was most abundant in the germ cell population and was present at significantly lower levels in the somatic cells. By contrast, the HSP84 protein was detected in the somatic cells of the testis rather than in germ cells. The steady-state levels of HSP86 and HSP84 paralleled the pattern of the expression of their respective mRNAs, suggesting that regulation at the level of translation was not a major mechanism controlling hsp90 gene expression in testicular cells. Immunoprecipitation analysis revealed that a 70-kDa protein coprecipitated with the HSP86/HSP84 proteins in testicular homogenates. This protein was identified as an HSP70 family member by immunoblot analysis, suggesting that HSP70 and HSP90 family members interact in testicular cells. © 1993Wiley-Liss, Inc.
    Zusätzliches Material: 4 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/dvg.1020140206
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  • 98
    Digitale Medien
    Digitale Medien
    Lack of concordance between heat shock proteins and the development of tolerance to teratogen-induced neural tube defects (1993)
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 14 (1993), S. 137-147 
    Details
    ISSN: 0192-253X
    Schlagwort(e): Heat-shock proteins ; teratogenicity, tolerance and cross-tolerance ; neural tube defects ; gene expression ; In situ transcription ; Life and Medical Sciences ; Genetics
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie
    Notizen: The present study was undertaken to examine the role of heat shock response in the development of tolerance and cross-tolerance in an in vivo murine model of teratogen-induced neural tube defects. The experimental paradigm designed to address this question was to utilize inbred mouse strains that differed in their sensitivity to hyperthermia and valproic acid induced neural tube defects, subjecting the dams to subteratogenic pretreatments with either heat or valproic acid at two different timepoints during development prior to the administration of the teratogenic insult. A statistically significant reduction in the frequency of neural tube defects and/or embryolethality following a pretreatment in dams subsequently exposed to a teratogenic treatment was considered evidence for the induction of tolerance. This was observed in the SWV embryos exposed to the 38°C pretreatment at 8:06 and to embryos exposed to either pretreatment temperature at 8:10 priorto a teratogenic heat shock at 8:12. In the LM/Bc embryos, only the 41°C pretreatment at 8:06 induced thermotolerance. There was no evidence of tolerance induced in either mouse strain using valproic acid. On the other hand, cross-tolerance was clearly demonstrated in this study, with a low temperature (41°C) pretreatment successfully protecting SWV fetuses from a subsequent teratogenic treatment with valproic acid, while valproic acid (200 mg/kg) was effective in reducing the risk of hyperthermia-induced neural tube defects in the LM/Bc fetuses. In all instances, tolerance was induced in the absence of significant induction of hsp synthesis. The lack ofconcordance between hsps and thermotolerance suggests that some other factor(s) is involved in conferring thermotolerance on developing murine embryos. © 1993 Wiley-Liss, Inc.
    Zusätzliches Material: 2 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/dvg.1020140208
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  • 99
    Digitale Medien
    Digitale Medien
    Erratum (1993)
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 14 (1993), S. 249-249 
    Details
    ISSN: 0192-253X
    Schlagwort(e): Life and Medical Sciences ; Genetics
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/dvg.1020140311
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    Digitale Medien
    Digitale Medien
    Differential induction of regulatory genes during mesoderm formation in Xenopus laevis embryos (1993)
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 14 (1993), S. 204-211 
    Details
    ISSN: 0192-253X
    Schlagwort(e): Inductive cell interactions ; diffusible molecules ; animal explants ; growth factors ; cyclo-heximide ; Life and Medical Sciences ; Genetics
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie
    Notizen: Mesoderm development in Xenopus laevis depends on inductive cell interactions mediated by diffusible molecules. The mesoderm inducer activin is capable of redirecting the development of animal explants both morphologically and biochemically. We have studied the induction of four regulatory genes, Mix. 1, goosecoid (gsc), Xlim-1 and Xbra in such explants by activin, and the influence of other factors on this induction. Activin induction of gsc is strongly enhanced by dorsalization of the embryo by LiCl, while expression of the other genes is only slightly enhanced. The protein synthesis inhibitor cycloheximide (CHX) inhibits the activin-dependent induction of Xbra partially, while induction of Mix. 1 and Xlim- 1 is essentially unaffected. In contrast, gsc shows strong superinduction in the presence of activin and CHX, and can be induced in animal explants by CHX alone. Induction and superinduction by CHX have previously been observed for immediate early genes in a variety of systems, notably for the activation of c-fos expression by serum stimulation, but have not been reported in early amphibian embryos. © 1993Wiley-Liss, Inc.
    Zusätzliches Material: 4 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/dvg.1020140307
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