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  • 1995-1999  (1,064)
  • 1995  (1,064)
  • Engineering General  (737)
  • Genetics  (327)
  • Nuclear reactions
  • 1
    ISSN: 1432-1440
    Keywords: Adrenergic receptors ; Human genetics ; Restriction fragment length polymorphism ; Chromosome mapping ; Linkage ; Genetics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract We have genetically mapped the genes encoding four human adrenergic receptors (ARs) of subtypes α1C, α2A, α2B, and β1, which are prototypic G protein coupled receptors that mediate the physiological effects of neurotransmitters, hormones, and drugs. We placed these genes onto the Cooperative Human Linkage Center (CHLC) and Genethon framework maps, within confidence intervals with greater than 1000∶1 odds. With multipoint analysis the α1C gene (locus ADRA1C) mapped to the interval between NEFL and D8S283; α2-C4, the gene encoding the α2C AR (locus ADRA2C), mapped to the interval between D4S126 and D4S62; and the α2-C10 (α2A AR)/β1 haplotype (loci ADRA2A/ ADRB1) mapped to the interval between D10S259 and D10S187. A fifth AR gene, β2, yielded significant LOD scores with markers on the long arm of chromosome 5; however, this locus (ADRB2) could not be mapped to any specific interval with odds of greater than 1000∶1. The two AR genes that are completely linked, α2-C10 and β1, were oriented on their shared 225-kb genomic fragment relative to the direction of transcription, with β1 being 5′ to α2-C10. The positioning of these genes on high-density framework maps allows them to be tested as candidates in a spectrum of diseases that might involve AR dysfunction.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Journal of neurology 242 (1995), S. 508-511 
    ISSN: 1432-1459
    Keywords: Dystonia ; Torticollis ; Blepharospasm ; Genetics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The inheritance of focal dystonias was investigated in 43 families containing 43 index cases with torticollis (n = 21), blepharospasm (n = 18) and writer's cramp (n = 4). They generated a potential population of 235 first-degree relatives, and 168 out of 179 living first-degree relatives were examined. Ten relatives with dystonia were identified in ten families. Another two parents from two of the same group of ten families were affected according to the family history. The majority of the secondary cases (six patients, five siblings, and one child) were not aware of any dystonia. The tendency for affected relatives to have the same type of dystonia as index patients was observed only for torticollis. Overall, 23% of index patients had relatives with dystonia. Segregation analysis suggested the presence of an autosomal dominant gene or genes with reduced penetrante underlying focal dystonia.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-1440
    Keywords: Atherosclerosis ; Hypertension ; Type 2 diabetes ; Genetics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract In the search for new risk factors for diabetic macroangiopathy the insertion/deletion (I/D) polymorphism in the angiotensin-converting enzyme gene was studied in 237 consecutive patients (125 men and 112 women) with non-insulin-dependent diabetes. The female population showed an excess of ischemic electro-cardiographic changes or definite myocardial infarctions in the patients homozygous for the deletion [D/D; odds ratio (OR) 2.8; 95% confidence interval (CI) 1.4–5.3] and in the insertion/deletion heterozygotes (I/D; OR 1.8; CI 1.1–3.1) compared with the patients homozygous for the insertion (I/I). In the total series coronary heart disease, cerebrovascular disease, and claudication were more often observed in the patients with I/D (OR 1.5; CI 1.0–2.2) or the D/D genotype patients (OR 1.7; CI 1.1–2.6) than in those with the genotype I/I. The systolic blood pressure was lower in patients with genotype I/I (138±19 mmHg) than in those with the genotype I/D (149±22 mmHg) or D/D (150±21 mmHg; P〈0.02). The prevalence of hypertension and the median urinary albumin excretion rate also tended to be lowest in the I/I genotype patients. Multiple logistic analysis revealed that in women the angiotensin-converting enzyme D/D genotype is independently associated with coronary heart disease. Our findings suggest that variation at the angiotensin-converting enzyme gene locus is one of the factors involved in the predisposition of diabetic patients to the development of arterial disease and hypertension.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Der Hautarzt 46 (1995), S. 394-399 
    ISSN: 1432-1173
    Keywords: Schlüsselwörter Malignes Melanom ; Genetische Instabilität ; Genetik ; Syndrom der dysplastischen Nävi ; Xeroderma pigmentosum ; Key words Malignant melanoma ; Genetic instability ; Genetics ; Dysplastic nevus syndrome ; Xeroderma pigmentosum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary Exposure of the skin to ultraviolet irradiation is an important risk factor for the development of malignant melanoma, with UVA possibly playing an important role. Hereditary factors are also relevant. In the dysplastic nevus syndrome a genetic instability has been shown by different methods. In xeroderma pigmentosum the DNA repair defect is thought to be responsible for the high incidence of malignant melanoma. Frequent and non-random changes in certain chromosomes have been demonstrated in melanoma cells. These might contain sequences that control melanoma growth or melanoma suppressor genes. Especially the short arm of chromosome 9 is thought to contain one of these genes. This hypothesis is supported by a genetic linkage analysis in melanoma families and the demonstration of a germ line deletion of the locus 9p21 in a patient with eight primary melanomas. Changes in known tumor suppressor genes and oncogenes have also been reported in melanoma, but no consistent sequence of genetic events is known.
    Notes: Zusammenfassung Die Exposition der Haut mit ultravioletten Strahlen ist ein wichtiger Risikofaktor für die Entwicklung eines malignen Melanoms. Möglicherweise spielt hierbei UVA-A eine besondere Rolle. Daneben sind hereditäre Faktoren von Bedeutung. Während beim Syndrom der dysplastischen Nävi eine genetische Instabilität mit verschiedenen Methoden nachgewiesen wurde, wird bei Xeroderma pigmentosum der DNA-Reparaturdefekt für die hohe Melanominzidenz verantwortlich gemacht. In Melanomzellen sind überzufällig häufig karyotypische Veränderungen in bestimmten Chromosomen gefunden worden. Diese enthalten möglicherweise Melanomwachstumsregulierende Sequenzen oder Melanom-Suppressorgene. Insbesondere der kurze Arm des Chromosoms 9 steht in Verdacht, eines dieser Gene zu enthalten. Diese Hypothese wird auch unterstützt durch eine genetische Kopplungsanalyse an Melanomfamilien und dem Nachweis einer Keimbahndeletion des Lokus 9p21 bei einer Patientin mit 8 primären Melanomen. Veränderungen an bereits bekannten Tumorsuppressorgenen oder Onkogenen sind ebenfalls in Melanomen beschrieben worden, ohne daß jedoch eine konsistente Reihenfolge von genetischen Ereignissen bekannt wäre.
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  • 5
    ISSN: 1432-1076
    Keywords: Key words Osteogenesis imperfecta ; Collagen I ; Mosaicism ; Genetics ; Recurrence risk
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Osteogenesis imperfecta (OI), a dominantly inherited connective tissue disorder, is usually caused by defects in collagen I. There is growing evidence for parental mosaicism that results in affected children born to unaffected parents. This situation poses a difficult task for the geneticist because a mosaic parent may appear clinically healthy while carrying the mutation in a fraction of her or his gonadal cells. To illustrate this problem, we report a Swiss couple whose first child was affected with severe OI. The unexpected recurrence of the disorder in the second child raised the suspicion of a recessive trait or, rather, of parental mosaicism. We identified the responsible collagen mutation in the COL1A2 gene (Gly688Ser in the α2(I)-chain) in both children and demonstrated the father to be a somatic mosaic for this mutation and to have subtle clinical signs such as soft skin and short stature that may be a result of his mosaic state.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    European journal of pediatrics 154 (1995), S. 654-657 
    ISSN: 1432-1076
    Keywords: Key words Fetal development ; Brain diseases ; Genetics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract We report two female siblings with the fetal brain disruption sequence. Extensive investigation of both children failed to define a definitive aetiology but clinical and laboratory findings are consistent with a hitherto unknown storage disease. We postulate that the accumulation of a neurotoxic metabolite may be responsible for the disease phenotype observed. This is the first report of recurrence of the fetal brain disruption sequence and supports the existence of a genetic form of this condition. Previous reports have emphasized possible environmental aetiologies. Infants with fetal brain disruption sequence should be investigated exhaustively and, in the absence of definitive evidence of an environmental cause, the possibility of a genetic aetiology should be considered. In some families the recurrence risk may be as high as one in four.
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  • 7
    ISSN: 1432-1076
    Keywords: Floating-Harbor syndrome ; Growth retardation ; Dysmorphology ; Genetics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Abstract The Floating-Harbor syndrome is a growth retardation syndrome with delayed bone age, speech development, and typical facial features. The face is triangular with deep-set eyes, long eyelashes, bulbous nose, wide columella, short philtrum, and thin lips. We present an additional patient and review 16 cases from the literature. The possible phenotype in the patient's mother suggests a dominant mode of inheritance for the syndrome. Conclusion The Floating Harbor syndrome is a growth deficiency syndrome characterized by proportionate short stature, characteristic face and delayed speech development. Inheritance is possibly autosomal dominant.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    European journal of pediatrics 154 (1995), S. 654-657 
    ISSN: 1432-1076
    Keywords: Fetal development ; Brain diseases ; Genetics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract We report two female siblings with the fetal brain disruption sequence. Extensive investigation of both children failed to define a definitive aetiology but clinical and laboratory findings are consistent with a hitherto unknown storage disease. We postulate that the accumulation of a neurotoxic metabolite may be responsible for the disease phenotype observed. This is the first report of recurrence of the fetal brain disruption sequence and supports the existence of a genetic form of this condition. Previous reports have emphasized possible environmental aetiologies. Infants with fetal brain disruption sequence should be investigated exhaustively and, in the absence of definitive evidence of an environmental cause, the possibility of a genetic aetiology should be considered. In some families the recurrence risk may be as high as one in four.
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 1432-1076
    Keywords: Key words Floating-Harbor ; syndrome ; Growth retardation ; Dysmorphology ; Genetics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The Floating-Harbor syndrome is a growth retardation syndrome with delayed bone age, speech development, and typical facial features. The face is triangular with deep-set eyes, long eyelashes, bulbous nose, wide columella, short philtrum, and thin lips. We present an additional patient and review 16 cases from the literature. The possible phenotype in the patient's mother suggests a dominant mode of inheritance for the syndrome. Conclusion The Floating Harbor syndrome is a growth deficiency syndrome characterized by proportionate short stature, characteristic face and delayed speech development. Inheritance is possibly autosomal dominant.
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 1432-0533
    Keywords: Primitive neuroepithelial tumor ; Desmoplastic small cell tumor ; Brain tumor of infancy Immunocytochemistry ; Genetics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract We describe a case of a desmoplastic brain tumor which was initially resected from the right fronto-temporal region in a 2 year-old boy. This nodular, calcified tumor was vascularized by the internal carotid artery and the middle meningeal artery branches. Grossly, it contained several mucoid cysts. Light microscopy showed cords or nests of small cuboidal cells surrounded by a loose connective tissue and desmoplasic areas containing fibers and spindle cells. The cuboidal cells expressed epithelial, neuronal and neuroendocrine markers. Some foci of spindle cells showed glial differentiation. The tumor recurred 16 months later and displayed some characteristics of the small cell neuroepithelial component, mitoses being conspicuous. Electron microscopy revealed undifferentiated clear cells, some containing neurosecretory granules. Karyotyping demonstrated the following formula: 〈 15 〉 46, t(8;11) (a13; q11). The chromosome 11 breakpoint was different from that described in Ewing's sarcoma. This isolated translocation has not been previously reported to our knowledge. These unusual features lead us to report this case and to discuss its pathogenesis.
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  • 11
    ISSN: 1432-2072
    Keywords: Opioid ; Genetics ; Self-administration ; CXBK/ByJ ; Reinforcement
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract It is commonly thought thatμ-receptors play an important role in the reinforcing effects of opioids. In the present study, inbred strains widely divergent in CNS opiate receptor densities were used to investigate the influence of genetic variation in receptor concentration on opioid-reinforced behavior. In particular, the CXBK/ByJ mice were used as an investigative tool because of their significantly lower number of CNSμ opioid receptors. The behavioral pharmacology of opioids in theμ-deficient CXBK/ByJ mice was compared to other commonly used inbred mouse strains, C57BL/6J and BALB/cJ, and the opiate receptor rich CXBH/ByJ mice. Operant opioid reinforced behavior, opioid-induced locomotor stimulation, analgesia and respiratory depression were investigated in all four inbred strains. To assess the acquisition and maintenance of opioid reinforced behavior, oral self-administration of the potent benzimidazole opioid, etonitazene, was determined using an operant fixed-ratio schedule of reinforcement (FR 8). Acquisition of etonitazene-reinforced behavior was established in all four strains including theμ-deficient CXBK/ByJ mice. However, there were significant genetic differences in the amount of drug intake during the maintenance of opioid-reinforced behavior and extinction behavior following vehicle substitution. For example, drug intake was significantly greater in the BK versus BH mice during the maintenance phase and an extinction burst was seen in the BH but not the BK mice following vehicle substitution. Thus,μ-receptor density may not account for individual variability in the acquisition of opioid-reinforced behavior under these conditions. Sensitivity to etonitazene-induced respiratory depression, stimulation of locomotor activity and analgesia were unrelated to drug intake during self-administration sessions across these four inbred strains. These data indicate that inherited differences in CNSμ-opiate receptor concentrations do not affect acquisition of etonitazene-reinforced behavior.
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  • 12
    Electronic Resource
    Electronic Resource
    Springer
    Child's nervous system 11 (1995), S. 453-455 
    ISSN: 1433-0350
    Keywords: Myotonic ; Dystrophy ; Muscle disease ; Genetics ; Case report
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract A large Sicilian kinship in which myotonic dystrophy (DM) affected spanning four generations is presented. The pedigree clearly illustrates the phenomenon of anticipation, and illustrates that this phenomenon is more marked when transmission occurs through an affected female rather than an affected male. The pedigree is interpreted in light of recent genetic advances in DM. Neurosurgeons and neurologists should consider a diagnosis of DM when asked to evaluate a floppy infant with enlarged lateral ventricles, and should be aware of special features regarding its inheritance pattern.
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  • 13
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 90 (1995), S. 380-388 
    ISSN: 1432-2242
    Keywords: Genetics ; Breeding ; Sorghum bicolor Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The objective of this study was to use restriction fragment length polymorphisms (RFLPs) to determine the genetic location and effects of genomic regions controlling plant height in sorghum. F2 plants (152) from the cross CK60 x PI229828 were used. Genomic and cDNA clones (106) identified 111 loci distributed among ten linkage groups covering 1299 cM. Interval mapping identified four regions, each in a separate linkage group. These regions may correspond to loci (dw) previously identified by alleles with qualitative effects. Also, these regions identified in sorghum may be orthologous to those previously reported for plant height in maize. Gene effects and gene action varied among genomic regions. In each region, PI229828 alleles resulted in increased plant height. Each region accounted for 9.2–28.7% of the phenotypic variation. Positive, additive effects ranged from 15 to 32cm. Tallness was dominant or overdominant and conferred by alleles from PI229828 for three quantitative trait loci (QTL). At the fourth QTL, PI229828 contributed to increased plant height, but short stature was partially dominant. One digenic interaction was significant. The presence of a PI229828 allele at one region diminished the effects of the other region. A multiple model indicated that these four regions collectively accounted for 63.4% of the total phenotypic variation. The utility of this information for germplasm conversion through backcross breeding is discussed.
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  • 14
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 90 (1995), S. 707-713 
    ISSN: 1432-2242
    Keywords: Mineral stress ; Nutrient efficiency ; Aluminium tolerance ; Inheritance ; Genetics ; Breeding
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The Brazilian tropical adapted soybeans contains, in addition to superior morphological characters, genetic factors for tolerance to cultivation in acidic, mineral-stressed soils. However, the selection process for these hindrances has been empirical, and information on the genetics of mineral element uptake by the plant is necessary. The objective of this investigation was to identify the mode of inheritance for the absorption of phosphorus, potassium, calcium, magnesium, iron, aluminium, manganese, zinc and copper in a 9 × 9 diallel cross. General combining ability (GCA) was higher than specific combining ability (SCA), with the exception of copper, manganese and zinc, indicating predominantly additive effects. The ratios of GCA/SCA varied between 3.4 (calcium) and 8.5 (magnesium). The regression of covariance (Wr) on variance (Vr) showed that the additive-dominance model explained the genetic differences in this germ plasm. However, the detection of overdominance could be related to possible heterozygosity in the parental varieties for mineral absorption. Broad-sense heritability values were higher than narrow sense heritability values for aluminium, iron, potassium, calcium and magnesium, being in the range of 67.9–86.9% and 42.0–56.6%, respectively. This is an indication that soybeans can be further improved to efficient utilisation of nutrients and to tolerate toxic factors in the soil.
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  • 15
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 90 (1995), S. 146-149 
    ISSN: 1432-2242
    Keywords: Capsicum chinense ; Resistance gene ; Genetics ; Pepper ; Tomato spotted wilt virus ; Tospoviruses
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Pepper (Capsicum chinense Jacq.) has been reported to be an important reservoir of resistance genes to tomato spotted wilt virus (TSWV). The genes for TSWV resistance present in three C. chinense lines (‘PI 152225’, ‘PI 159236’ and ‘Panca’) were investigated for allelism. All resistant lines were crossed with each other. Parents, F1, backcrosses and F2 populations (including reciprocals) developed from those crosses were mechanically inoculated with a highly virulent TSWV isolate. Susceptible C. annuum cv ‘Magda’ was used to check inoculum virulence. Fifty plants of the F1 hybrids; ‘Magda’ x ‘PI 152225’, ‘Magda’ x ‘PI 159236’, and ‘Magda’ x 'Panca, were also inoculated with the TSWV isolate. The resistance response in all C. chinense sources was associated with a localized, hypersensitive-like reaction that was phenotypically expressed as a prompt formation of large local lesions accompanied by premature leaf abscission. All F1 generations presented a final score of resistant; indicating that the expression of resistance to TSWV is conditioned by a dominant gene regardless of the source. The absence of segregation for resistance to TSWV that was observed in all generations of the crosses between C. chinense lines indicated that either a tightly linked group of genes exists or that the resistance is governed by the same single major gene (probably the already described Tsw gene). Previous reports have indicated that the Tsw gene is not effective against tospovirus members of serogroup II, i.e. tomato chlorotic spot virus (TCSV) and groundnut ring spot virus (GRSV). In the assay described here, all of the C. chinense lines showed, after mechanical inoculation, an identical susceptibility response to the TCSV and GRSV isolates.
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  • 16
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 91 (1995), S. 639-646 
    ISSN: 1432-2242
    Keywords: Gene introgression ; Genetics ; Linkage ; Taxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The wild tetraploid (2n=28) oat species Avena magna and A. murphyi have been domesticated by having been transferred from the common oat, A sativa (2n=42), the characteristics of non-shedding spikelets glabrous and yellow lemma, and reduced awn formation. Domestication has been achieved by crossing the common oat with either of the tetraploid species and then backcrossing the pentaploid hybrids with pollen of the tetraploid wild parent. Among the BC plants obtained only a few produced some seeds. Fertile tetraploids exhibiting the domesticated syndrome have been selected for in the F2 generation. Although morphologically they were almost indistinguishable from the common oat, they were tetraploids. Wild x domesticated A. magna hybrids were vigorous and fertile. They retained their spikelets at maturity, lemma color and pubescence were intermediate between the parental lines, and awns were formed only on the lower floret of the spikelet. Each of these characteristics segregated in a 3∶1 fashion, indicating single gene control, as in the common oat. These four characteristics formed a linkage group in one F2 family and two linkage groups in the other two families. The usefulness of the domesticated tetraploids for oat research and production has been discussed. Taxonomically, the domesticated tetraploids were ranked as subspecies: A. magna ssp. domestica, and A. murphyi ssp. rigida.
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  • 17
    ISSN: 1432-5233
    Keywords: Maturity-onset diabetes of the young (MODY) ; Genetics ; Diabetes mellitus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Genetic linkage studies of families with earlyonset type 2 diabetes have facilitated the identification of diabetes-susceptibility genes. In order to assess the feasibility of using linkage approaches to identify genes responsible for the development of type 2 diabetes in Japanese subjects, we examined our clinical records for multigenerational families suitable for genetic studies. We identified 16 families in which at least one subject was diagnosed with type 2 diabetes before 25 years of age. Seven of these families had a pattern of inheritance consistent with a diagnosis of maturity-onset diabetes of the young (MODY) and nine families showed a complex pattern of inheritance of type 2 diabetes with transmission of diabetes-susceptibility genes from both parents. The glucokinase and mitochondrial tRNALeu(UUR) genes were screened for mutations in at least one affected subject from each family in order to assess the contribution of mutations in these genes to the development of the diabetes. No mutations were found, which suggests that the diabetes in these families resulted from mutations in other genes.
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  • 18
    Electronic Resource
    Electronic Resource
    Springer
    Sexual plant reproduction 8 (1995), S. 129-132 
    ISSN: 1432-2145
    Keywords: Sex control ; Disomic segregation ; Dioecy Kiwifruit ; Genetics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The sex segregation ratio was checked in bi-parental families of Actinidia deliciosa (2n=6x=174) obtained by crossing four females (A12, Mo3, Br4, Hw1) with two males (T2, M1) and one fruiting male (M3h, subandroecious) according to a factorial mating design. The M3h fruiting male was also selfed. The sex ratio was checked in maternal families of A. kolomikta (2n=2x) and A. chinensis (2n=2x) as well as in A. deliciosa. Seedlings of both diploid species took 3–4 years to progress beyond juvenility, whereas a noticeable number of seedlings from biparental crosses of A. deliciosa involving A12 and Hw1 as seed parents were still non-flowering after seven growing seasons. Open-pollinated families of both diploid and hexaploid species as well as most families from biparental crosses showed a sex segregation ratio approaching 1∶1. Subandroecious lines with different degrees of ovary and pistil development appeared in proportions of 0–4.2%, depending on the cross, but only 6 of the 2567 male vines checked were capable of setting fruit. No case of self-fertility or apomixis was detected among 1866 bagged female vines. Selfed M3h progenies gave only female and male phenotypes in a ratio of 1 female to 3 males. No off-type vines were found among these progenies. The same disomic sex segregation ratio seems to be operating at different ploidy levels in the genus Actinidia. Since selfed fruiting males produced both female and male individuals, the male sex appears to be the heterogametic one. Such evidence indicates that a monofactorial system based on one or more linked genes or on an X/Y chromosome set must be controlling sex expression. How a monofactorial sex-determining mechanism could operate in polyploids to give a 1∶1 female: male ratio is discussed. Minor modifying gene(s) seem to be responsible for the feminization of males, and their expression appears enhanced by environmental conditions. Masculinizing gene(s) seem to be lacking in female genotypes.
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  • 19
    ISSN: 1432-2072
    Keywords: Cocaine ; Quantitative trait loci ; Seizure ; Recombinant inbred strains ; Genetics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Among inbred mice, genetic factors mediate differences in sensitivity to the convulsant properties of cocaine; however, the gene(s) underlying cocaine's effects have not been identified. To help elucidate the gene(s) responsible for cocaine seizure susceptibility, we used recombinant inbred-quantitative trait loci (RI-QTL) analyses to identify chromosomal loci associated with cocaine-induced seizures. RI-QTL analyses seek to identify associations between a quantitative measure of a particular phenotype and one or more previously mapped marker genes across a panel of RI strains. This report describes an RI-QTL analysis of cocaine seizure susceptibility among 26 BXD RI strains. These strains showed a skewed, bimodal range of seizure susceptibility which could be the result of one or more modifying genes acting in concert with a major gene to influence cocaine sensitivity. Correlating the percent seizures displayed by each strain following 60 mg/kg cocaine with chromosomal marker data for these strains revealed a number of significant correlations clustered in two regions on chromosomes 12 and 6. This is the first identification of putative chromosomal loci associated with a cocaine-related phenotype and should facilitate identification of the gene(s) underlying cocaine toxicity and other cocaine-related phenotypes.
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  • 20
    Electronic Resource
    Electronic Resource
    Springer
    Rheumatology international 15 (1995), S. 89-93 
    ISSN: 1437-160X
    Keywords: SLE Lupus ; BBV transformation ; Genetics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Epstein-Barr-virus (EBV)-transformed lymphoblastoid B-cell lines were generated from peripheral blood lymphocytes of 55 patients with systemic lupus erythematosus (SLE) and 44 healthy relatives. All donors have previously been extensively characterized with regard to clinical, serologic, and genetic parameters. Here, peripheral blood lymphocytes and lines were characterized for cell surface antigens. Furthermore, autoantibody production and proliferation rate of the cell lines were monitored. A significant difference between patients and relatives was the lower proliferation rate of EBV-transformed cell lines of the SLE patients. All SLE cell lines are available for interested researches and can be obtained from the European Cell Bank, Salisbury, UK.
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  • 21
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical medicine and bioethics 16 (1995), S. 347-373 
    ISSN: 1573-1200
    Keywords: Genetics ; human research ; adolescence ; child ; informed consent ; decision making ; medical ethics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Philosophy
    Notes: Abstract The participation of adolescents in genetic research engenders unusual problems concerning the nature of their informed consent. In this study we analyze 70 consent documents collected from genetics investigators in the United States who conduct research with children and adolescents. We find that many consent documents do not reflect either the current or the developing ethical and legal standards for research with adolescents and that in many cases the documents are simply confusing or unclear. We make recommendations for change to reflect more adequately the changing perspective concerning the autonomous decision-making capacity of adolescents.
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  • 22
    Electronic Resource
    Electronic Resource
    Springer
    Environmental biology of fishes 43 (1995), S. 1-27 
    ISSN: 1573-5133
    Keywords: Conservation ; Extinction ; Rarity ; Biodiversity ; Breeding guilds ; Endemism ; Speciation ; Habitat degradation ; Environmental management ; Invasive fishes ; Genetics ; Ecology ; Stenotopy ; Captive propagation ; Legislation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Synopsis The conservation status and factors threatening fishes worldwide are reviewed in order to introduce a series of one-page articles on ‘Threatened fishes of the world’, and to encourage the incorporation of information on threatened fishes into international conservation programmes. Information on fish extinction and threat rates are compared with those of other animal groups, and the unique characteristics of fish conservation problems are highlighted. At present 979 species of fishes are listed as threatened in the IUCN Red List and at least 36 species and three subspecies are listed as recently extinct. It is argued that these figures are probably gross underestimates and that they may mislead conservation authorities and resource users about the seriousness of the situation. Freshwater fishes may be the most threatened group of vertebrates after the Amphibia. Urgent action is required to save many narrowly endemic, stenotopic species from extinction, especially in Africa, Asia and South America. The conservation of common species that drive essential ecological processes is also important. Anthropogenic pressures, especially habitat degradation, the introduction of invasive species and pollution, on inland and coastal waters are particularly severe and many major fish communities are threatened with elimination throughout the world. The conservation of marine fishes is complicated by the fact that it is difficult to ascertain their rarity. The importance of the retention of genetic variation is highlighted, and both orthodox and innovative conservation measures are encouraged. Further research on minimum viable populations, genetics, and the factors that cause fishes to become vulnerable to extinction, is urgently required.
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  • 23
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    Springer
    Journal of industrial microbiology and biotechnology 14 (1995), S. 355-364 
    ISSN: 1476-5535
    Keywords: Transformation ; Fungi ; Yeast ; Genetics ; Biotechnology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The genetic investigation of fungi has been extended substantially by DNA-mediated transformation, providing a supplement to more conventional genetic approaches based upon sexual and parasexual processes. Initial transformation studies with the yeastSaccharomyces cerevisiae provided the model for transformation systems in other fungi with regard to methodology, vector construction and selection strategies. There are, however, certain differences betweenS. cerevisiae and filamentous fungi with regard to type of genomic insertion and the availability of shuttle vectors. Single-site linked insertions are common in yeast due to the high level of homology required for recombination between vectored and genomic sequences, whereas mycelial fungi often show a high frequency of heterologous and unlinked insertions, often in the form of random and multiple-site integrations. While extrachromosomally-maintained or replicative vectors are readily available for use with yeasts, such vectors have been difficult to construct for use with filamentous fungi. The development of vectors for replicative transformation with these fungi awaits further study. It is proposed that replicative vectors may be inherently less efficient for use with mycelial fungi relative to yeasts, since the mycelium, as an extended and semicontinuous network of cells, may delimit an adequate diffusion of the vector carrying the selectable gene, thus leading to a high frequency of abortive or unstable transformants.
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  • 24
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    International Journal for Numerical Methods in Fluids 20 (1995), S. 319-334 
    ISSN: 0271-2091
    Keywords: Chemically reactive flows ; Finite element analysis ; Polymeric fluids ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: We consider steady state and time-dependent flows of chemically reactive polymeric systems in two-dimensional geometries. A numerical simulation tool is proposed for predicting the evolution of the macroscopic velocity, temperature, stress and species concentration fields in such flows. We formulate a general mathematical model on the basis of the first principles of continuum mechanics, which includes a description of the non-liner coupling between kinematics, heat transfer and chemical kinetics. The resulting set of non-linear partial differential equations is solved numerically by means of appropriate finite element techniques. We have implemented the resulting numerical model in the general-purpose POLYFLOWR software developed in Louvain-la-Neuve, Belgium. Simulation results for various steady state and time-dependent reactive flows are reported.
    Additional Material: 18 Ill.
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  • 25
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    International Journal for Numerical Methods in Fluids 20 (1995), S. 337-339 
    ISSN: 0271-2091
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
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  • 26
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    International Journal for Numerical Methods in Fluids 20 (1995), S. 341-361 
    ISSN: 0271-2091
    Keywords: Navier-Stokes ; unsteady ; composite multigrid ; incompressible ; non-staggered grid ; semi-implicit ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: A time-accurate, finite volume method for solving the three-dimensional, incompressible Navier-Stokes equations on a composite grid with arbitrary subgrid overlapping is presented. The governing equations are written in a non-orthogonal curvilinear co-ordinate system and are discretized on a non-staggered grid. A semi-implicit, fractional step method with approximate factorization is employed for time advancement. Multigrid combined with intergrid iteration is used to solve the pressure Poisson equation. Inter-grid communication is facilitated by an iterative boundary velocity scheme which ensures that the governing equations are well-posed on each subdomain. Mass conservation on each subdomain is preserved by using a mass imbalance correction scheme which is secondorder-accurate. Three test cases are used to demonstrate the method's consistency, accuracy and efficiency.
    Additional Material: 16 Ill.
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  • 27
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    International Journal for Numerical Methods in Fluids 20 (1995), S. 393-414 
    ISSN: 0271-2091
    Keywords: shallow water equations ; spectral element ; implicit scheme ; GMRES solver ; staggered mesh ; North Atlantic ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: A staggered spectral element model for the solution of the oceanic shallow water equations is presented. We introduce and compare both an implicit and an explicit time integration scheme. The former splits the equations with the operator-integration factor method and solves the resulting algebraic system with generalized minimum residual (GMRES) iterations. Comparison of the two schemes shows the performance of the implicit scheme to lag that of the explicit scheme because of the unpreconditioned implementation of GMRES. The explicit code is successfully applied to various geophysical flows in idealized and realistic basins, notably to the wind-driven circulation in the North Atlantic Ocean. The last experiment reveals the geometric versatility of the spectral element method and the effectiveness of the staggering in eliminating sprious pressure modes when the flow is nearly non-divergent.
    Additional Material: 12 Ill.
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  • 28
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    International Journal for Numerical Methods in Fluids 20 (1995), S. 597-598 
    ISSN: 0271-2091
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
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  • 29
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    International Journal for Numerical Methods in Fluids 20 (1995), S. iii 
    ISSN: 0271-2091
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
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  • 30
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    International Journal for Numerical Methods in Fluids 20 (1995), S. 459-468 
    ISSN: 0271-2091
    Keywords: heat transfer ; turbulent flow ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: The accurate modelling of heat transfer to turbulent flow and the prediction of the temperature distribution in the flow remain one of the problem areas of numerical simulations. Traditional turbulence closure models, like the k-ε model, effectively only increase the viscosity of the fluid and introduce wall functions close to boundaries to obtain the correct velocity distribution. These turbulence models do not model the small-scale mixing that occurs in turbulent flow. When solving the energy equation these small-scale mixings dominate the heat transfer rate at the boundaries as well as the temperature distribution in the flow. This paper outlines a revised method, based on the k-ε turbulence model, that can be used to predict heat transfer in turbulent flow. A single turbulent conductivity term is introduced that can be used over the complete flow field including the boundaries. A detailed description of the mathematical model and boundary conditions used for the turbulence model are included in the paper. The effective turbulent conductivity method was evaluated in several finite difference simulations of water flowing through a smooth pipe while being heated. Simulation and verification were performed over a range of Reynolds numbers. Verification of the model is accomplished by comparing the numerically predicted centre temperature of the fluid as well as the heat flux to the fluid to measured temperatures in a similar pipe. From these results it is concluded that the revised turbulent conductivity model holds great potential to obtain accurate simulated heat transfer rates for general applications.
    Additional Material: 7 Ill.
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  • 31
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    International Journal for Numerical Methods in Fluids 20 (1995), S. 541-557 
    ISSN: 0271-2091
    Keywords: electromagnetic induction heating ; inverse method ; finite element ; coupled fields ; experiment simulation ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: The induction heating model described herein couples the standard heat conduction equation with electro-magnetic proximity-skin equations. An Inverse Finite Element procedure, which is based on prior deterministic and probabilistic concepts, has been designed to solve the inherent inverse equation model with respect to the unknown coil current parameter. Simulated experiments using different noises in the input data have been performed in order to determine their influence on the estimated parameter. The IFEM has shown its capability to predict the optimal location for the temperature sensors, together with their numbers, consistently with a pre-specified estimate accuracy. Specifically, only one temperature sensor, located in the middle of the two turns of the coil, results to be sufficient to estimate the unknown parameter to a satisfactory accuracy degree. This, may significantly help to design optimal experiments.
    Additional Material: 10 Ill.
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  • 32
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    International Journal for Numerical Methods in Fluids 20 (1995), S. 507-522 
    ISSN: 0271-2091
    Keywords: radiative ignition ; pyrolysing solid fuels ; coal ; numerical model ; numerical method of lines ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: A numerical model of radiative ignition of pyrolysing solid fuels is developed. The model is one-dimensional and transient. The following mechanisms are simultaneously accounted for: (i) the surface heat and mass transport, (ii) the surface oxidation chemical reaction, (iii) the in-depth pyrolysis, (iv) the gas-phase heating by absorption of the radiation and by heat conduction/convection from the solid surface, and (v) the gas-phase chemical reaction. The solutions are obtained numerically with the method of lines. Using lignite and bituminous coal for the simulations, the results confirm that the pyrolysis products absorb a significant amount of the external radiation. Predictions of the ignition times show that both the surface ignition time and the gas-phase ignition time decrease rapidly with increasing radiation intensities. A good agreement between predictions and experiments is obtained.A sensitivity analysis is also carried out with the key kinetic parameters. This analysis establishes an upper limit for surface and pyrolysis activation energies and a lower limit for gas-phase activation energy. Within these limits, the radiative ignition of coals appears as an integration of two consecutive ignition modes: the surface ignition occurs first, which is then followed by the gas-phase ignition. Beyond these limits, the single gas-phase ignition mode is the only ignition mode to prevail.
    Additional Material: 8 Ill.
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  • 33
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    International Journal for Numerical Methods in Fluids 20 (1995), S. 559-572 
    ISSN: 0271-2091
    Keywords: melting ; natural convection ; body-fitted co-ordinates ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: A numerical study of natural convection melting of a phase change material within an isothermal vertical cylinder was conducted. The governing conservation equations are formulated in terms of a stream function, vorticity and temperature. Body-fitted co-ordinates are employed for tracking the irregular shape of the timewise changing solid-liquid phase front. Results show that the convective flow patterns and time evolution of the phase front, resulting from simultaneous bottom, side and top heating, are far more complicated than those for the melting from a single isothermal boundary. The heat transfer rate at the top surface is found to decrease monotonically to zero as convection is fully developed in the melt. The highest heat transfer rates are observed at the bottom surface where Bénard convective cells develop. Due to the convective motion of the melt along the vertical heated wall, the onset of Bénard convection occurs at a much earlier time than that for the case of melting within a cylinder heated from below.
    Additional Material: 6 Ill.
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  • 34
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    International Journal for Numerical Methods in Fluids 20 (1995) 
    ISSN: 0271-2091
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
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  • 35
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    International Journal for Numerical Methods in Fluids 20 (1995), S. 641-648 
    ISSN: 0271-2091
    Keywords: impinging jet ; uniform suction ; turbulence model ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: The paper presents numerical predictions of a turbulent axisymmetric jet impinging onto a porous plate, based on a finite volume method of solving the Navier-Stokes equations for an incompressible air jet with the K-ε turbulence model. The velocity and pressure terms of the momentum equations are solved by the SIMPLE (semi-implicit method for pressure-linked equation) method. In this study, non-uniform staggered grids are used. The parameters of interest include the nozzle-to-wall distance and the suction velocity. The results of the present calculations are compared with available data reported in the literature. It is found that suction effects reduce the boundary layer thickness and increase the velocity gradient near the wall.
    Additional Material: 6 Ill.
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  • 36
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    International Journal for Numerical Methods in Fluids 20 (1995) 
    ISSN: 0271-2091
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
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  • 37
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    International Journal for Numerical Methods in Fluids 20 (1995), S. 1153-1161 
    ISSN: 0271-2091
    Keywords: finite element ; arterial flow ; magnetic field ; co-ordinate transformation ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: A finite element solution of the Navier-Stokes equations for steady flow under the magnetic effect through a double-branched two-dimensional section of a three-dimensional model of the canine aorta is discussed. The numerical scheme involves transforming the physical co-ordinates to a curvilinear boundary-fitted co-ordinate system. The shear stress at the wall is calculated for a Reynolds number of 1000 with the branch-to-main aortic flow rate ratio as a parameter. The results are compared with earlier works involving experimental data and found to be in reasonable qualitative agreement. The steady flow, shear stress and branch flow under the effect of a magnetic field have been discussed in detail.
    Additional Material: 9 Ill.
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  • 38
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    International Journal for Numerical Methods in Fluids 20 (1995), S. 1179-1197 
    ISSN: 0271-2091
    Keywords: adaptive grids ; equidistribution ; compressible viscous aerodynamics ; CFD modelling ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: A technique is described for the adaptation of a structured control volume mesh during the iterative solution process of the Navier-Stokes equations. The scalar equidistribution method is adopted, in conjunction with a Laplace-like grid solver to make a curvilinear body-fitted grid sensitive to local flow gradients. Hence, whilst the total number of grid nodes remains constant during a computation, their relative position is continuously adjusted to promote clustering of cells in regions where gradients are high. The focus of this work is in compressible aerodynamics, where such clustering would be desirable in regions containing shocks but also in boundary layers. The technique is three-dimensional and operates in a series of user-defined grid subdomains or patches. These patches act as reference frames within which grid activity takes place. Bi-cubic splines are extensively used to define the aerodynamic surfaces forming the calculation boundaries and to ensure that grid movement does not compromise surface integrity. The technique is applied to aerofoils, wing surfaces, transonic ducts and nozzles and a supersonic wedge cascade. Significant sharpening of both normal and oblique shock discontinuities is demonstrated over static grid simulations and with fewer overall grid nodes. The technique is successful in both inviscid and viscous (turbulent) simulations.
    Additional Material: 16 Ill.
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  • 39
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    International Journal for Numerical Methods in Fluids 20 (1995), S. 1215-1236 
    ISSN: 0271-2091
    Keywords: square obstruction ; channel flow ; vortex shedding ; sliding walls ; numerical calculation ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Results are presented for the unsteady, two-dimensional flow and heat transfer due to a square obstruction of diameter d located asymmetrically between the parallel sliding walls of a channel with length-to-height ratio W/H = 6·44. Analysis is based on the numerical solution of spatially and temporally second-order accurate finite difference approximations of the transport equations expressed in curvilinear co-ordinates. Laminar, constant property flow is assumed for obstruction configurations in which the blockage ratio is d/H = 0·192, the nearest-wall distances are g/d = 0·2, 0·5 and 1, the orientation angles are α=0°, 10° and 20° and the Reynolds numbers are Re=100, 500, and 1000. Preparatory testing of the numerical procedure was performed for a variety of documented flows to verify its physiconumerical accuracy and obtain estimates of the residual grid-dependent uncertainties in the variables calculated. Heat transfer, drag and lift coefficients and Strouhal numbers for the present flow were finally calculated to within 4%-7% of their grid-dependent values using non-uniformly spaced grids consisting of (x=99, y=55) nodes. Above a critical value of the Reynolds number, which depends on the geometrical parameters, the flow is characterized by alternate vortex shedding from the obstruction top and bottom surfaces. Streamline, vorticity and particle streakline plots provide qualitative impressions of the unsteady vortical flow. Especially noteworthy are the extremes in the lift coefficient which ranges from large positive values for an obstruction with g/d=0·2 and α=10° to negative values for one with g/d=0·5 and α=0°. Both the drag and lift coefficients as well as the Strouhal number exhibit non-monotonic variations with respect to the parameters explored. Asymmetries in the obstruction location and orientation account for relatively large vortex-induced periodic variations in heat transfer, especially along the wall nearest the obstruction. Notable differences are also predicted for the heat transfer coefficients of the individual obstruction surfaces as a function of the orientation angle.
    Additional Material: 13 Ill.
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  • 40
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    International Journal for Numerical Methods in Fluids 20 (1995), S. 1293-1314 
    ISSN: 0271-2091
    Keywords: chemical reactors ; annular liquid jets ; grid generation ; mass absorption ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: A numerical study of Hopf bifuractions in annular liquid jets with mass transfer is presented. The study is based on the asymptotic equations which govern the dynamics of inviscid, incompressible, thin, annular liquid jets and on equilibrium conditions for mass transfer at the jet's inner and outer interfaces. It is shown that the amplitude of the time-periodic motion that results from the Hopf bifurcation increases whereas its frequency decreases as the solubility ratio is increased.
    Additional Material: 19 Ill.
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  • 41
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    International Journal for Numerical Methods in Fluids 20 (1995), S. 1363-1380 
    ISSN: 0271-2091
    Keywords: volume-of-fluid ; free surface flows ; interface advection ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: A numerical technique is developed for the simulation of free surface flows and interfaces. This technique combines the strength on the finite element method (FEM) in calculating the field variables for a deforming boundary and the versatility of the volume-of-fluid (VOF) technique in advection of the fluid interfaces. The advantage of the VOF technique is that it allows the simulation of interfaces with large deformations, including surface merging and breaking. However, its disadantage is that is solving the flow equations, it cannot resolve interfaces smaller than the cell size, since information on the subgrid scale is lost. Therefore the accuracy of the interface reconstruction and the treatment of the boundary conditions (i.e. viscous stresses and surface tension forces) become grid-size-dependent. On the other hand, the FEM with deforming interface mesh allows accurate implementation of the boundary conditions, but it cannot handle large surface deformations occurring in breaking and merging of liquid regions. Combining the two methods into a hybrid FEM-VOF method eliminates the major shortcomings of both. The outcome is a technique which can handle large surface deformations with accurate treatment of the boundary conditions. For illustration, two computational examples are presented, namely the instability and break-up of a capillary jet and the coalescence collision of two liquid drops.
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  • 42
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    International Journal for Numerical Methods in Fluids 20 (1995), S. 671-693 
    ISSN: 0271-2091
    Keywords: airfoil ; artificial viscosity ; upwinding ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: The numerical solution of the compressible Euler and Navier-Stokes equations in primitive variables form requires the use of artificial viscosity or upwinding. Methods that are first-order-accurate are too dissipative and reduce the effective Reynolds number substantially unless a very fine grid is used. A first-order finite element method for the solution of the Euler and Navier-Stokes equations can be constructed by adding Laplacians of the primitive variables to the governing equations. Second-order schemes may require a fourth-order dissipation and higher-order elements. A finite element approach is proposed in which the fourth-order dissipation is recast as the difference of two Laplacian operators, allowing the use of bilinear elements. The Laplacians of the primitive variables of the first-order scheme are thus balanced by additional terms obtained from the governing equations themselves, tensor identities or other forms of nodal averaging. To demonstrate formally the accuracy of this scheme, an exact solution is introduced which satisfies the continuity equation identically and the momentum equations through forcing functions. The solutions of several transonic and supersonic inviscid and laminar viscous test cases are also presented and compared to other available numerical data.
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  • 43
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    International Journal for Numerical Methods in Fluids 20 (1995), S. 743-776 
    ISSN: 0271-2091
    Keywords: computational aerodynamics ; shock capturing ; positive schemes ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: A unified theory of non-oscillatory finite volume schemes for both structured and unstructured meshes is developed in two parts. In the first part, a theory of local extremum diminishing (LED) and essentially local extremum diminishing (ELED) schemes is developed for scalar conservation laws. This leads to symmetric and upstream limited positive (SLIP and USLIP) schemes which can be formulated on either structured or unstructured meshes. The second part examines the application of similar ideas to the treatment of systems of conservation laws. An analysis of discrete shock structure leads to conditions on the numerical flux such that stationary discrete shocks can contain a single interior point. The simplest formulation which meets these conditions is a convective upwind and split pressure (CUSP) scheme, in which the coefficient of the pressure differences is fully determined by the coefficient of convective diffusion. Numerical results are presented which confirm the properties of these schemes.
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  • 44
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    International Journal for Numerical Methods in Fluids 20 (1995), S. 803-817 
    ISSN: 0271-2091
    Keywords: unstructured grids ; finite element ; turbulence ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: An unstructured finite element method is presented for calculation of turbulent flow fields about aerospace configurations. Algebraic, one-equation, and two-equation turbulence models are implemented and compared. A new procedure for implementing an unstructured algebraic model without an auxiliary structured grid is presented. The overall procedure is applied to simulation of flow about launch vehicle configurations. The turbulence models are evaluated for calculation of flow fields about a forebody with shock induced separation. For this case. the one-equation model gives better predictions. An inviscid flow field about a complete launch vehicle with multiple boosters is also evaluated to demonstrate the overall procedure.
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  • 45
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    International Journal for Numerical Methods in Fluids 20 (1995), S. 853-868 
    ISSN: 0271-2091
    Keywords: numerical schemes ; non-linear hyperbolic systems ; Reimann solvers ; adaptive meshes ; unsteady flows ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: This paper discusses the different sources of non-physical entropy production which occur in the numerical resolution of the Euler equations for compressible inviscid flow and proposes several ways of correcting these effects. In particular a hybrid corrected centred, augmented by an accurate upwind scheme near singular boundaries is proposed which satisfies the mathematical entropy condition, and which solves the flow accurately within regions near non-smooth boundaries of the computational domain. A coupled new dynamic auto-adaptive mesh algorithm which produces highly accurate solutions is also introduced. This algorithm is non-hierarchical. i.e. it does not depend on a fixed background mesh, which allows structural and geometrical changes and generates extremely precise discretizations for steady and unsteady flow.
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  • 46
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    International Journal for Numerical Methods in Fluids 20 (1995), S. 915-934 
    ISSN: 0271-2091
    Keywords: optimum design ; mesh adaptivity ; adaptive remeshing ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: This paper presents a methodology for solving shape optimization problems in the context of fluid flow problems including adaptive remeshing. The method is based on the computation of the sensitivities of the geometrical design parameters, the mesh, the flow variables and the error estimator to project the refinement parameters from one design to the next. This sensitivity analysis is described for the incompressible potential equations and the Euler equations. The efficiency of the proposed method is checked by means of two 2D inverse problems.
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  • 47
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    International Journal for Numerical Methods in Fluids 20 (1995), S. 989-1001 
    ISSN: 0271-2091
    Keywords: streamline diffusion ; finite element ; Lagrangian method ; space-time discretization ; 3D flow ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: In this paper we describe a space-time finite element method, with elements aligned along the computed characteristics in space-time, for the computation of incompressible free surface flows in three dimensions.
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  • 48
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    International Journal for Numerical Methods in Fluids 20 (1995), S. 443-458 
    ISSN: 0271-2091
    Keywords: finite elements ; advection-diffusion ; diffusion errors ; dispersion errors ; stability ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Various finite element schemes of the Bubnov-Galerkin and Taylor-Galerkin types are analysed to obtain the expressions of truncation errors. This way, dispersion errors in the transient, and diffusion errors both in the transient and in the steady state, are identified. Then, with reference to the transient advection-diffusion equation, stability limits are determined by means of a general von Neumann procedure. Finally, the operational equivalence between Taylor-Galerkin methods, utilized for pseudo-transient calculations, and Petrov-Galerkin methods, derived for the steady state forms of the advection-diffusion equation, is illustrated. Theoretical conclusions are supported by the results of numerical experiments.
    Additional Material: 2 Ill.
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  • 49
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    International Journal for Numerical Methods in Fluids 20 (1995), S. 469-492 
    ISSN: 0271-2091
    Keywords: boundary layer ; heat transfer ; turbulence ; CFD ; finite elements ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: This paper presents an efficient finite element method for solving the unsteady Navier-Stokes equations for turbulent incompressible flow coupled with thermal problems. This method has been implemented in the N3S code, developed at Electricité de France. The time discretization is first described. We precise then the Chorin and the ‘projected Uzawa’ algorithms used for the Stokes problem. Recent improvements concerning the optimization of finite element calculations are also detailed. The second part deals with the modelling of the thermal boundary layer used to simulate walls with fixed temperature in turbulent flows. The differences with other modelling suggested in the literature are discussed. The last part presents some applications. EDF is involved in the conception of heating or cooling systems and numerical methods constitute a very useful tool to study the movements of air in habitations. The calculations are validated by comparisons with measurements.
    Additional Material: 14 Ill.
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  • 50
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    International Journal for Numerical Methods in Fluids 20 (1995), S. 493-506 
    ISSN: 0271-2091
    Keywords: finite element method ; mould filling ; explicit Taylor-Galerkin ; pseudo-concentration ; interface elements ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: A model for simulating the process of mould filling in castings is presented. Many defects in a casting have their origins at the filling stage. Numerical simulation of this process can be of immense practical benefit to the foundry industry, however a rigorous analysis of this process must model a wide range of complex physical phenomena. In order to contain the costs and complexity that would be necessary for such a model, certain simplifying assumptions have been made. These assumptions limit the scope of this model to only predicting realistic thermal fields during the filling process.A laminar regime has been assumed for the flow field, which is obtained by solving the incompressible Navier-Stokes equations using a velocity-pressure segregated semi-implicit finite element method. The free metal surface is predicted by advecting a pseudo-concentration function via the computed flow field. This involves an explicit finite element solution of a pure advection equation. The thermal field is calculated by solving the convective-diffusive energy equation by an explicit finite element method using the computed flow field and the location of the free surface. All the advection terms are discretized using a Taylor-Galerkin method. The interface between the metal and mould is modelled using special interface elements.The model is demonstrated by solving practical example problems. The results show that a sharp thermal front is maintained during the course of filling without excessive diffusion. The heat diffusion in the mould can be controlled by varying the metal mould heat transfer coefficient.
    Additional Material: 6 Ill.
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  • 51
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    International Journal for Numerical Methods in Fluids 20 (1995), S. 599-601 
    ISSN: 0271-2091
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
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  • 52
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    International Journal for Numerical Methods in Fluids 21 (1995), S. 91-92 
    ISSN: 0271-2091
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
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  • 53
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    International Journal for Numerical Methods in Fluids 21 (1995), S. 93-111 
    ISSN: 0271-2091
    Keywords: smallest drag ; first-order necessary condition ; second-order necessary condition ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: The problem of finding the shape of a body with smallest drag in a flow governed by the two-dimensional steady Navier-Stokes equations is considered. The flow is expressed in terms of a streamfunction which satisfies a fourth-order partial differential equation with the biharmonic operator as principal part. Using the adjoint variable approach, both the first- and second-order necessary conditions for the shape with smallest drag are obtained. An algorithm for the calculation of the optimal shape is proposed in which the first variations of solutions of the direct and adjoint problems are incorporated. Numerical examples show that the algorithm can produce the optimal shape successfully.
    Additional Material: 7 Ill.
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  • 54
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    International Journal for Numerical Methods in Fluids 21 (1995), S. 129-139 
    ISSN: 0271-2091
    Keywords: numerical technique ; boundary layer equations ; reacting flow ; full coupling ; finite rate chemistry effects ; thermal radiation effects ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: A new numerical scheme for reacting axisymmetric jet flows formed between a fuel jet and co-flowing air has been developed. The model is mathematically described by a set of non-linear parabolic partial differential equations in two space dimensions, i.e. the boundary layer equations. The numerical scheme that the programme uses for solving the fully coupled conservation equations of mass, momentum, energy and species is a generaliztion of the discretization technique recently developed by Villasenor (J. Math. Comput. Simul., 36, 203-208 (1994)). Chemical production (and destruction) of the species is allowed to occur through N elementary reversible (or irreversible) reactions involving k species, although in the present model the reaction rates are evaluated with a simplified kinetic mechanism for a one-step global reaction. Thermal radiation is considered assuming an optically thin limit and adopting the grey medium approximation. Allowances are made for natural convection effects and variable thermodynamic and molecular transport properties. The performance of the model in solving the coupled aerodynamic and finite rate chemistry effects is tested by comparing model predictions with experimental data of Mitchell et al. (Combust. Flame, 37, 227-244 (1980)) for a buoyant, laminar, diffusion axisymmetric methane-air flame.
    Additional Material: 4 Ill.
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  • 55
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    International Journal for Numerical Methods in Fluids 21 (1995), S. 155-180 
    ISSN: 0271-2091
    Keywords: computational fluid dynamics ; finite-difference method ; Navier-Stokes equations ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: The disarrangement of a perturbed lattice of vortices was studied numerically. The basic state is an exponentially decaying, exact solution of the Navier-Stokes equations. Square arrays of vortices with even numbers of vortex cells along each side were perturbed and their evolution was investigated. Whether the energy in the perturbation grows somewhat before it decays or decays monotonically depends on the initial strength of the vortices of the basic state, the extent of lateral confinement and the structure of the perturbation. The critical condition for temporally local instability, i.e. the critical amplitude of the basic state that must be exceeded to allow energy transfer from the basic state to the perturbation, is discussed. In the strongly confined case of a square lattice of four vortices the appearance of enchancement of global rotation is the result of energy transfer from the basic state to a temporally local unstable mode. Energy is transferred from the basic state to larger-scaled structures (inverse cascade) only if the scales of the larger structures are inherently contained in the initial structure of the perturbation. The initial structure of the double array of vortices is not maintained except for a very special form of perturbation. The facts that large scales decay more slowly than small scales and that, when non-linearities are sufficiently strong, energy is transferred from one scale to another explain the differences in the disarrangement process for different initial strengths of the vortices of the basic state. The stronger vortices, i.e. the vortices perturbed in a manner that increases their strength, tend to dominate the weaker vortices. The pairing and subsequent merging (or capture) of vortices of like sense into larger-scale vortices are described in terms of peaks in the evolution of the square root of the palinstrophy divided by the enstrophy.
    Additional Material: 21 Ill.
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  • 56
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    International Journal for Numerical Methods in Fluids 21 (1995), S. 369-370 
    ISSN: 0271-2091
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
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  • 57
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    International Journal for Numerical Methods in Fluids 21 (1995), S. 401-411 
    ISSN: 0271-2091
    Keywords: unsteady incompressible flow ; vorticity-velocity formulation ; numerical simulation ; staggered grid ; spherical Couette flow ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: A new numerical method for solving the axisymmetric unsteady incompressible Navier-Stokes equations using vorticity-velocity variables and a staggered grid is presented. The solution is advanced in time with an explicit two-stage Runge-Kutta method. At each stage a vector Poisson equation for velocity is solved. Some important aspects of staggering of the variable location, divergence-free correction to the velocity field by means of a suitably chosen scalar potential and numerical treatment of the vorticity boundary condition are examined. The axisymmetric spherical Couette flow between two concentric differentially rotating spheres is computed as an initial value problem. Comparison of the computational results using a staggered grid with those using a non-staggered grid shows that the staggered grid is superior to the non-staggered grid. The computed scenario of the transition from zero-vortex to two-vortex flow at moderate Reynolds number agrees with that simulated using a pseudospectral method, thus validating the temporal accuracy of our method.
    Additional Material: 4 Ill.
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  • 58
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    International Journal for Numerical Methods in Fluids 20 (1995), S. 1315-1336 
    ISSN: 0271-2091
    Keywords: numerical wave tank ; multi-subdomain approach ; non-linear water waves ; wave breaking ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: An efficient 2D non-linear numerical wave tank called LONGTANK has been developed based on a multi-subdomain (MSD) approach combined with the conventional boundary element method (BEM). The multi-subdomain approach aims at optimized matrix diagonalization, thus minimizing the computing time and reserved storage. The CPU per time step in LONGTANK simulation is found to increase only linearly with the number of surface nodes, which makes LONGTANK highly efficient especially when simulating long-time wave evolutions in space.Appropriate treatment of special points on the boundary ensures high resolution in LONGTANK simulation beyond initial deformation and breaking, which allows detailed study of breaking criterion, breaker morphology, breaking dissipation, vorticity generation, etc.Detailed numerical implementation has been given with demonstration of LONGTANK simulations.
    Additional Material: 17 Ill.
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  • 59
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    International Journal for Numerical Methods in Fluids 20 (1995), S. 1381-1382 
    ISSN: 0271-2091
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
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  • 60
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    International Journal for Numerical Methods in Fluids 21 (1995) 
    ISSN: 0271-2091
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
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  • 61
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    International Journal for Numerical Methods in Fluids 21 (1995), S. 571-590 
    ISSN: 0271-2091
    Keywords: CDF ; predictions ; laser Doppler anemometry ; inlet port ; cylinder ; turbulence ; steady flow ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: This paper presents a combined experimental and computational study of the steady flow through an internal combustion engine inlet port. The port was of generic design with a straight centreline. The three-dimensional velocity and turbulence fields in the port and cylinder were simulated using a computational fluid dynamics programme. Laser sheet flow visualization and laser Doppler anemometry were also employed to investigate the flows and assess the predictions. The results show that a large-scale flow structure is created in the cylinder by the inlet jet and its interaction with the valve and cylinder walls. Both predictions and measurements show that the flow is strongly dependent on the valve lift but is not affected by the flow rate. Comparisons of the numerical predictions with the experimental data indicated that the mean flow features are accurately predicted in many parts of the flow field; some discrepancies are evident and stem primarily from the failure of the simulation to predict a small recirculation region in the port which affects the trajectory of the annular jet entering the cylinder. Calculations were also made without modelling the port shape by using simplified inlet conditions upstream of the valve seat. It was found that this approximation can provide a reasonable, albeit less accurate, description of the flow, but modelling of the port shape is necessary for accurate flow predictions.
    Additional Material: 18 Ill.
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  • 62
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    International Journal for Numerical Methods in Fluids 20 (1995), S. 831-851 
    ISSN: 0271-2091
    Keywords: error estimation ; adaptivity ; hp-methods ; Navier-Stokes ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Recently, a rigorous a posteriori error estimate, based on the element residual method, for the steady-state Navier-Stokes equations has been derived. In this paper, by using this error estimate, we construct an hp adaptive strategy to minimize the total computation costs while achieving a targeted accuracy for steady incompressible viscous flow problems. The basic hp adaptive strategy is to solve the approximate problem in three consecutive stages corresponding to three different meshes, i.e. an initial mesh, an intermediate adaptive h-mesh, and a final adaptive hp mesh. Our numerical result shows that the three-step hp adaptive strategy for the incompressible flow problems indeed provides an accurate approximate solution while keeping the computational costs under control.
    Additional Material: 13 Ill.
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  • 63
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    International Journal for Numerical Methods in Fluids 21 (1995), S. 697-698 
    ISSN: 0271-2091
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
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  • 64
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    International Journal for Numerical Methods in Fluids 21 (1995), S. 699-722 
    ISSN: 0271-2091
    Keywords: domain decomposition ; free-surface ; ship flow ; viscous flow ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: An application of multidomain decomposition to the computation of the steady free surface flow past a ship hull is presented. Viscous effects are taken into account in the neighbourhood of solid walls and in the wake by the Reynolds averaged Navier-Stokes equations, whereas the assumption of irrotationality in the external flow allows a description by a potential model. Free surface boundary conditions have been implemented in a linearized form at the undisturbed waterplane. Suitable matching conditions are enforced at the interface between the viscous and the potential regions. The numerical results obtained for two merchant ship forms (the HSVA tanker and the Series 60 hull) are compared with experimental data available in the literature.
    Additional Material: 23 Ill.
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  • 65
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    International Journal for Numerical Methods in Fluids 21 (1995), S. 763-780 
    ISSN: 0271-2091
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: This paper describes a calculation technique to determine the linear instability characteristics of jets of arbitrary exit geometry. In particular, elliptic and rectangular jets are considered. The numerical procedure involves both a conformal transformation between the computational domain and the physical plane and a solution of the transformed stability equation in the computational domain. Modern, efficient, conformal mappings are used for both simply and doubly connected domains. The numerical solution is based on a hybrid finite difference/pseudospectral discretization of the stability equation. The technique is validated by comparison with previous stability calculations for circular and elliptic jets. Calculations are performed for the stability characteristics of elliptic and rectangular jets of aspect ratio 2:1. Growth rates, phase velocities, and pressure eigenfunctions are presented.
    Additional Material: 9 Ill.
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  • 66
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    International Journal for Numerical Methods in Fluids 21 (1995), S. iii 
    ISSN: 0271-2091
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
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  • 67
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    International Journal for Numerical Methods in Fluids 21 (1995), S. 837-856 
    ISSN: 0271-2091
    Keywords: stabilized finite elements ; projection method ; approximate projections ; equal-order interpolation ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: In an attempt to overcome some of the well-known ‘problems’ with the Q1P0 element, we have devised two ‘stabilized’ versions of the Q1Q1 element, one based on a semi-implicit approximate projection method and the other based on a simple forward Euler technique. While neither one conserves mass in the most desirable manner, both generate a velocity field that is usually ‘close enough’ to divergence-free. After attempting to analyse the two algorithms, each of which includes some ad hoc ‘enhancements’, we present some numerical results to show that they both seem to work well enough. Finally, we point out that any projection method that uses a ‘pressure correction’ approach is inherently limited to time-accurate simulations and, even if treated fully implicitly, is inappropriate for seeking steady states via large time steps.
    Additional Material: 8 Ill.
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  • 68
    ISSN: 0271-2091
    Keywords: wavelet ; multiple scale methods ; optimal dilation parameter ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Multiple scale methods based on reproducing kernel and wavelet analysis are developed. These permit the response of a system to be separated into different scales. These scales can be either the wave numbers corresponding to spatial variables or the frequencies corresponding to temporal variables, and each scale response can be examined separately. This complete characterization of the unknown response is performed through the integral window transform, and a space-scale and time-frequency localization process is achieved by dilating the flexible multiple scale window function. An error estimation technique based on this decomposition algorithm is developed which is especially useful for local mesh refinement and convergence studies. This flexible space-scale window function can be constructed to resemble the well-known unstructured multigrid and hp-adaptive finite element methods. However, the multiple scale adaptive refinements are performed simply by inserting nodes into the highest wavelet scale solution region and at the same time narrowing the window function. Hence hp-like adaptive refinements can be performed without a mesh. An energy error ratio parameter is also introduced as a measure of aliasing error, and critical dilation parameters are determined for a class of spline window functions to obtain optimal accuracy. This optimal dilation parameter dictates the number of nodes covered under the support of a given window function. Numerical examples, which include the Helmholtz equation and the 1D and 2D advection-diffusion equations, are presented to illustrate the high accuracy of the methods using the optimal dilation parameter, the concept of multiresolution analysis and the meshless unstructured adaptive refinements.
    Additional Material: 17 Ill.
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  • 69
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    International Journal for Numerical Methods in Fluids 21 (1995), S. 955-966 
    ISSN: 0271-2091
    Keywords: Laplace transform ; combined method ; transient problem ; chemical reaction ; convective diffusion ; infinite region ; finite element method ; boundary element method ; coupling method ; unsteady problem ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: A numerical method for solving the problem of transient convective diffusion with a first-order chemical reaction is presented in this paper. The method is applicable over an infinite region. For steady problems the combined method of finite and boundary elements is recognized as a successful numerical technique for dealing with an infinite region. The present method is also useful in transient problems. In order to formulate the combined method for transient problems, we have developed a new method. In this paper the Laplace transform method incorporating the combined finite and boundary element methods will be considered. This transformation, holding complex values, transforms the transient problem into a steady state form. We also consider the present numerical solution which is obtained by using the numerical inverse Laplace transform as presented by Hosono. In numerical experiments the present method gives us an extremely accurate solution.
    Additional Material: 11 Ill.
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  • 70
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    International Journal for Numerical Methods in Fluids 21 (1995), S. 1007-1014 
    ISSN: 0271-2091
    Keywords: finite element ; massively parallel ; coupled flow ; baroclinic annulus waves ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Coupled, three-dimensional, time-dependent, incompressible flows in a differentially heated, rotating annulus are simulated using a parallel implementation of the Galerkin finite element method on the Connection Machine 5 (CM-5) supercomputer. The development of baroclinic annulus waves is computed and found to be consistent with previous experimental reseults. The implementation of a repeated spectral bisection element-partitioning technique significantly increases the computation speed over a strategy which randomly maps elements to processors, yielding sustained calculation rates of 8.1 GFLOPS on 512 processors of the CM-5.
    Additional Material: 2 Ill.
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  • 71
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    International Journal for Numerical Methods in Fluids 21 (1995), S. 141-153 
    ISSN: 0271-2091
    Keywords: incompressible flow ; artificial compressibility ; artificial bulk viscosity ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Peyret (J. Fluid Mech., 78, 49-63 (1976)) and others have described artificial compressibility iteration schemes for solving implicit time discretizations of the unsteady incompressible Navier-Stokes equations. Such schemes solve the implicit equations by introduing derivatives with respect to a pseudo-time variable τ and marching out to a steady state in τ. The pseudo-time evolution equation for the pressure p takes the form ∂p/∂ = -a2∂∇.u, where a is an artificial compressibility parameter and u is the fluid velocity vector. We present a new scheme of this type in which convergence is accelerated by a new procedure for setting a and by introducing an artificial bulk viscosity b into the momentum equation. This scheme is used to solve the non-linear equations resulting from a fully implicit time differencing scheme for unsteady incompressible flow. We find that the best values of a and b are generally quite different from those in the analogous scheme for steady flow (J. D. Ramshaw and V. A. Mousseau, Comput. Fluids, 18, 361-367 (1990)), owing to the previously unrecognized fact that the character of the system is profoundly altered by the pressence of the physical time derivative terms. In particular, a Fourier dispersion analysis shows that a no longer has the significance of a wave speed for finite values of the physical time step δt,. Inded, if on sets a ˜ |u| as usual, the artificial sound waves cease to exist when δt is small and this adversely affects the iteration convergence rate. Approximate analytical expressions for a and b are proposed and the benefits of their use relative to the conventional values a ∼ |u| and b = 0 are illustrated in simple test calculations.
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  • 72
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    International Journal for Numerical Methods in Fluids 21 (1995) 
    ISSN: 0271-2091
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
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  • 73
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    International Journal for Numerical Methods in Fluids 21 (1995), S. 205-222 
    ISSN: 0271-2091
    Keywords: two-phase ; Van Leer ; convective transport ; volume fractions ; momentum ; interface tracking ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: The Van Leer method for the computation of convective fluxes is extended to two-phase flow. By preventing spurious undershoots and overshoots, the scheme preserves physical realism while maintaining high-order accuracy. This is particulary important for two-phase flows, since phase exchange terms are typically a function of volume fraction products and numerical diffusion can incorrectly mix the two phases. The scheme described here is constructed to guarantee that the sum of the volume fractions is always unity and that the volume fractions are always greater than or equal to zero. Various test problems are computed to demonstrate the accuracy of the method and to show how the scheme might be incorporated in existing computational methods. In addition to multiphase flow applications, setting equal phase velocities results in a volume marker scheme that is well suited to single-phase interface tracking problems.
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  • 74
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    International Journal for Numerical Methods in Fluids 21 (1995), S. 237-251 
    ISSN: 0271-2091
    Keywords: finite volume ; multigrid ; unsteady flow ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: This paper presents results of the numerical study of a piston-driven unsteady flow in a pipe with sudden expansion. The piston closes the larger-diameter pipe and moves between two limiting positions with strong acceleration or deceleration at the beginning and end of each stroke and constant velocity in between. The piston velocity in the exhaust stroke is about four times higher than in the intake stroke. Periodic piston movement in this fashion creates a complex unsteady flow between the piston head and the plane of sudden expansion. The numerical method is implicit and of finite volume type, using a moving grid and a collocated arrangement of variables. Second-order spatial discretization, fine grids and a multigrid solution method were used to ensure high accuracy and good efficiency. Spatial and temporal discretization errors were of the order of 1% and 0.1% respectively. The features of the flow are discussed and the velocity profiles are compared with experimental data, showing good qualitative and quantitative agreement.
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  • 75
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    International Journal for Numerical Methods in Fluids 21 (1995), S. 253-278 
    ISSN: 0271-2091
    Keywords: volcanic conduits ; homogeneous flow ; exsolution ; total-variation-diminishing techniques ; method of characteristics ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: A one-dimensional, time-dependent, isothermal, homogeneous, two-phase flow model was developed to study magma ascent in volcanic conduits. The physical modeling equations were numerically solved by means of a TVD (total variation diminishing) predictor-corrector procedure and by means of a predictor-corrector technique based on the method of characteristics. The results from the transient model were verified with an analytical solution for wave propagation in conduits without friction and gravitational effects. The numerical solutions were also compared with those of a steady-state, homogeneous, two-phase model for basaltic and rhyolitic magma ascents in the fissures and circular conduits of Vesuvius and Mt St. Helens. An application of the model to magma decompression in conduits indicates very short times for gas exsolution, fragmentation, and shock wave propagation, implying that the modelling of gas exsolution should involve non-equilibrium kinetics effects. Future coupling of the transient magma ascent model with magma chamber and pyroclastic dispersion models should allow for more realistic simulations of the time-dependent behavior of real volcanic eruptions.
    Additional Material: 14 Ill.
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  • 76
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    International Journal for Numerical Methods in Fluids 21 (1995), S. 967-972 
    ISSN: 0271-2091
    Keywords: lattice gas model ; cellular automaton ; shear layer ; diffusion ; Kelvin-Helmholtz instability ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: The ‘two-colour lattice gas model’ is applied to the analysis of shear layers between two parallel flows with different velocities U1 and U2. Two cases, (a) U1 = 0.4, U2 = 0.2 and (b) U1 = 0.4, U2 = 0.0, are calculated and compared with the theoretical solutions. We obtain good agreement between theory and calculations in the velocity profiles of the shear layers. It is found that this model can simulate complicated physical phenomena of shear layers at the microscopic level.
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  • 77
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    International Journal for Numerical Methods in Fluids 21 (1995), S. 973-979 
    ISSN: 0271-2091
    Keywords: finite element method ; transient flow ; power-law fluid ; extrudate swell ; marker particles ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: A simple extrusion process for Newtonian and power-law fluids is analysed. Marker particles are introduced to analyse the fluid flow motions. Area co-ordinates of six-node triangular element are used to determine the marker position in the element. With this element, the solution algorithm becomes simple compared with the one using the linear triangular element. The differences in flow and swell patterns between the two fluids are described.
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  • 78
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    International Journal for Numerical Methods in Fluids 21 (1995), S. 1015-1016 
    ISSN: 0271-2091
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
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  • 79
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    International Journal for Numerical Methods in Fluids 21 (1995), S. 1017-1039 
    ISSN: 0271-2091
    Keywords: turbulence models ; flux-difference splitting ; multigrid method ; transonic turbulent flow ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: In this paper Roe's flux-difference splitting is applied for the solution of Reynolds-averaged Navier-Stokes equations. Turbulence is modelled using a low-Reynolds number form of the k-∊ tubulence model. The coupling between the turbulence kinetic energy equation and the inviscid part of the flow equations is taken into account. The equations are solved with a diagonally dominant alternating direction implicit (DDADI) factorized implicit time integration method. A multigrid algorithm is used to accelerate the convergence. To improve the stability some modifications are needed in comparison with the application of an algebraic turbulence model. The developed method is applied to three different test cases. These cases show the efficiency of the algorithm, but the results are only marginally better than those obtained with algebraic models.
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  • 80
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    International Journal for Numerical Methods in Fluids 21 (1995), S. 1067-1086 
    ISSN: 0271-2091
    Keywords: laminar flows ; incompressible flows ; pressure correction ; Krylov subspace methods ; approximate factorization ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: A segregated algorithm for the solution of laminar incompressible, two- and three-dimensional flow problems is presented. This algorithm employs the successive solution of the momentum and continuity equations by means of a decoupled implicit solution method. The inversion of the coefficient matrix which is common for all momentum equations is carried out through an approximate factorization in upper and lower triangular matrices. The divergence-free velocity constraint is satisfied by formulating and solving a pressure correction equation. For the latter a combined application of a preconditioning technique and a Krylov subspace method is employed and proved more effecient than the approximate factorization method. The method exhibits a monotonic convergence, it is not costly in CPU time per iteration and provides accurate solutions which are independent of the underrelaxation parameter used in the momentum equations. Results are presented in two- and three-dimensional flow problems.
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  • 81
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    International Journal for Numerical Methods in Fluids 21 (1995), S. 1087-1107 
    ISSN: 0271-2091
    Keywords: two-layer ; finite difference ; time splitting ; orthogonal curvilinear co-ordinates ; grid ‘block’ ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: A new two-layer, two-dimensional mathematical model employing a finite difference method based on numerically generated boundary-fitted orthogonal co-ordinates and a grid ‘block’ technique for unsteady boundary problems is developed which can be used to simulate flows with density stratification in a natural water-body with complicated topography. In the model the turbulent exchange across the interface is treated empirically and a time-splitting finite difference method with two fractional steps is employed to solve the governing equations. The model is calibrated and verified by comparing the computational results with data measured in Tolo Harbour, Hong Kong. The simulation results mimie the field measurements very closely. The computation shows that the model reproduces the two-layer, two-dimensional tidal flow with density stratification in Tolo Harbour very well. The computed velocity hodographs show that the tidal circulations at various positions in each layer have different patterns and that the features of the patterns are independent of the tidal type except for their scales. The computed Lagrangian pathlines show that the tidal excursion is dependent on the tidal type, especially in the inner harbour and side-coves.
    Additional Material: 16 Ill.
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  • 82
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    International Journal for Numerical Methods in Fluids 21 (1995), S. 1137-1151 
    ISSN: 0271-2091
    Keywords: transient planar flow ; viscoelastic material ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: In this study, we examine the numerical simulation of transient viscoelastic flows with two moving free surfaces. A modified Galerkin finite element method is implemented to the two-dimensional non-steady motion of the fluid of the Oldroyd-B type. The fluid is initially placed between two parallel plates and bounded by two straight free boundaries. In this Lagrangian finite element method, the spatial mesh deforms in time along with the moving free boundaries. The unknown shape of the free surfaces is determined with the flow field u, v, τ, p by the deformable finite element method, combined with a predictor-corrector scheme in an uncoupled fashion. The moving free surfaces and fluid motion of both Newtonian and non-Newtonian flows are investigated. The results include the influence of surface tension, fluid inertia and elasticity.
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  • 83
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    International Journal for Numerical Methods in Fluids 21 (1995), S. 1171-1200 
    ISSN: 0271-2091
    Keywords: implicit schemes ; non-symmetric matrices ; iterative procedures ; relaxation methods ; gradient methods ; quasinewton methods ; convection-diffusion equation ; Euler equation ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Various tests have been carried out in order to compare the performances of several methods used to solve the non-symmetric linear systems of equations arising from implicit discretizations of CFD problems, namely the scalar advection-diffusion equation and the compressible Euler equations. The iterative schemes under consideration belong to three families of algorithms: relaxation (Jacobi and Gauss-Seidel), gradient and Newton methods. Two gradient methods have been selected: a Krylov subspace iteration method (GMRES) and a non-symmetric extension of the conjugate gradient method (CGS). Finally, a quasi-Newton method has also been considered (Broyden). The aim of this paper is to provide indications of which appears to be the most adequate method according to the particular circumstances as well as to discuss the implementation aspects of each scheme.
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  • 84
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    International Journal for Numerical Methods in Fluids 21 (1995), S. 1041-1048 
    ISSN: 0271-2091
    Keywords: finite difference method (FDM) ; computational fluid dynamics ; transport equation ; numerical stability ; numerical oscillations ; characteristic equation ; LECUSSO scheme ; QUICK scheme ; LENS scheme ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: In order to obtain stable and accurate numerical solutions for the convection-dominated steady transport equations, we propose a criterion for constructing numerical schemes for the convection term that the roots of the characteristic equation of the resulting difference equation have poles.By imposing this criterion on the difference coefficients of the convection term, we construct two numerical schemes for the convection-dominated equations. One is based on polynomial differencing and the other on locally exact differencing.The former scheme coincides with the QUICK scheme when the mesh Reynolds number (Rm) is \documentclass{article}\pagestyle{empty}\begin{document}$\mathop \[{\textstyle{{\rm 8} \over {\rm 3}}}\] $\end{document}, which is the critical value for its stability, while it approaches the second-order upwind scheme as Rm goes to infinity. Hence the former scheme interpolates a stable scheme between the QUICK scheme at Rm = \documentclass{article}\pagestyle{empty}\begin{document}$\mathop \[{\textstyle{{\rm 8} \over {\rm 3}}}\] $\end{document} and the second-order upwind scheme at Rm = ∞. Numerical solutions with the present new schemes for the one-dimensional, linear, steady convection-diffusion equations showed good results.
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  • 85
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    International Journal for Numerical Methods in Fluids 21 (1995) 
    ISSN: 0271-2091
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
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  • 86
    ISSN: 0192-253X
    Keywords: Testis ACE ; positive promoter element ; in vitro transcription ; tissue-specificity ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Testis angiotensin-converting enzyme (testis ACE) is an isozyme of ACE only expressed by male germ cells during spermiogenesis. It is the result of a strong sperm-specific promoter found within the 12th intron of the somatic ACE gene. Previous studies have localized the boundaries of the mouse testis ACE promoter as being from -91 to -9, relative to the transcriptional start site, and have suggested two important DMA regulatory elements starting at positions -55 and -32. DNA constructs were made in which these motifs were either eliminated or substituted. Each construct was tested for its ability to promote transcription in vitro, using a rat testis nuclear extract. Disruption of either motif reduced in vitro transcription to about 30% of control levels, while mutations of both elements abolished transcription. Two sites were selected inside each motif and altered by point mutation. Each of four constructs, containing a mutation at -51, -48, -30, or -28, transcribed at 29% or less the efficiency of the parent construct. The DNA element at -55, TGAGGTCA, is homologous to a consensus cyclic AMP response element. The motif at -32, TCTTAT, is located at a position analogous to a TATA box. Substitution of the -32 motif with a consensus TATA box sequence, TATAAA, stimulated transcriptional activity about 3-fold. As measured by gel mobility shift, oligonucleotides encompassing the -32 motif and the consensus TATA box formed different DNA-protein complexes. However, the -32 motif oligonucleotide was recognized by nuclear proteins prepared from either liver or testis nuclei. In this example of a tissue-specific promoter that functions only during spermatogenesis, at least two DNA elements act synergistically during in vitro transcription. © 1995 Wiley-Liss, Inc.
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  • 87
    ISSN: 0192-253X
    Keywords: Human serum albumin ; β-lactoglobulin ; casein ; mammary gland ; transgenic mice ; developmental regulation ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: We compared the developmental pattern of expression of the sheep β-lactoglobulin (BLG), the chimeric BLG/human serum albumin (HSA), and the endogenous murine β-casein genes in the mammary gland of virgin, pregnant and lactating transgenic mice, both at the RNA (expression) and protein (synthesis and secretion) levels. The BLG and casein genes were expressed at very low levels in virgin animals and during early stages of pregnancy. The increase in the expression of these genes started at the second half of pregnancy and reached a peak between the end of pregnancy and day 10 of lactation. The accumulation of their RNA coincided with that of the corresponding proteins, indicating a transcriptional control of expression of these genes. The expression and secretion patterns of the endogenous casein gene in transgenic and nontransgenic mice were indistinguishable. The hybrid BLG/HSA gene constructs displayed distinct patterns of expression in virgin animals and at early stage of pregnancy, from that of the BLG transgene or the endogenous mouse milk protein gene. High levels of expression (17-60% of that on day 18 of pregnancy) were detected in the mammary gland of virgin animals. At day 5 of pregnancy there was a dramatic decrease in HSA synthesis and secretion in all transgenic strains tested. The down-regulation, revealed by immunoprecipitation and immunohistochemical studies, demonstrated that at that stage of pregnancy only 10-18% of ductal structures contained HSA expressing cells in contrast to the majority of ducts expressing HSA in virgin animals. These morphological studies also demonstrated that the down-regulation in HSA synthesis and secretion was correlated with the transition from ducts comprised of a single layer of epithelial cells (characteristic of the virgin state) to ducts composed of multilayers of such cells. In two of the three transgenic strains tested, the down-regulation at the protein level was associated with a similar decrease in HSA transcripts. In the exceptional strain no. 23, HSA transcripts continued accumulating even at this stage. The differences in the control of expression at the RNA level between these transgenic strains were also confirmed by in situ hybridization. Our results suggest the involvement of at least two regulatory mechanisms effective at early stages of gestation in the control of expression/secretion of the HSA transgene targeted for expression in the mammary gland by the BLG milk protein promoter. These putative mechanisms may play key roles in the interplay between normal mammogenesis and lactogenesis. Thus, transgenic mice expressing BLG/HSA gene constructs at early stages of gestation would be valuable in further dissecting these mechanisms. © 1995 Wiley-Liss, Inc.
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  • 88
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    Developmental Genetics 16 (1995), S. 298-310 
    ISSN: 0192-253X
    Keywords: Embryogenesis ; rice ; mutation ; phenotypic diversity ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: We have identified 188 embryo mutants of rice and characterized them into six groups based on their phenotypes: (1) embryoless in mature seed, (2) deletion of embryonic organ(s), (3) abnormal position of embryonic organs, (4) abnormal embryo size, (5) defect in organ morphology, and (6) variable abnormal phenotypes in spite of single mutations. Three types of organless mutants are obtained: small globular embryo, club-shaped embryo, and large embryo. Although 12 shootless mutants derived from at least three loci are identified, only three radicleless mutants are recovered, which produce normal adventitious roots after germination. In reduced embryo mutants, every embryonic organ is reduced, in contrast to giant embryo mutants in which only scutellum is enlarged. Considerable number of mutants are categorized into (5) and (6) in the above. These diverse embryo mutants would serve as promising materials for genetic study of embryogenesis. © 1995 Wiley-Liss, Inc.
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  • 89
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    Developmental Genetics 16 (1995), S. 344-348 
    ISSN: 0192-253X
    Keywords: knotted1 ; embryogenesis ; shoot apical meristem ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The formation of shoot and root meristems that ultimately give rise to all tissues of the plant body occurs for the first time during embryogenesis. Meristem formation has traditionally been defined in terms of the appearance of histological features of meristems; this approach has led to varying interpretations of the timing of meristem formation relative to other events in embryogenesis. Markers that would provide more objective criteria for the analysis of meristem formation have not been widely available. The maize homeobox gene, knotted1 (kn1), is expressed in shoot meristems throughout postembryonic stages of shoot development. In order to determine whether this gene is expressed in the shoot meristem from its earliest inception, we examined the expression of kn1 in embryos at a series of stages by in situ hybridization to kn1 mRNA and immunolocalization of KN1 protein. Our results show that the onset of kn1 expression is temporally and spatially coincident with the earliest histologically recognizable signs of shoot meristem formation in the embryo, and thus provides a valuable marker for this process. © 1995 Wiley-Liss, Inc.
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  • 90
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    Developmental Genetics 17 (1995), S. 1-5 
    ISSN: 0192-253X
    Keywords: Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
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  • 91
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    Developmental Genetics 17 (1995), S. 16-28 
    ISSN: 0192-253X
    Keywords: Mesoderm ; fate-mapping ; germ layer formation ; morphogenetic movement ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The developmental fate of cells in the epiblast of early-primitive-streak-stage mouse embryos was assessed by studying the pattern of tissue colonisation displayed by lac Z-expressing cells grafted orthotopically to nontransgenic embryos. Results of these fate-mapping experiments revealed that the lateral and posterior epiblast contain cells that will give rise predominantly to mesodermal derivatives. The various mesodermal populations are distributed in overlapping domains in the lateral and posterior epiblast, with the embryonic mesoderm such as heart, lateral, and paraxial mesoderm occupying a more distal position than the extraembryonic mesoderm. Heterotopic grafting of presumptive mesodermal cells results in the grafted cells adopting the fate appropriate to the new site, reflecting a plasticity of cell fate determination before ingression. The first wave of epiblast cells that ingress through the primitive streak are those giving rise to extraembryonic mesoderm. Cells that will form the mesoderm of the yolk sac and the amnion make up a major part of the mesodermal layer of the midprimitive-streak-stage embryo. Cells that are destined for embryonic mesoderm are still found within the epiblast, but some have been recruited to the distal portion of the mesoderm. By the late-primitive-streak-stage, the mesodermal layer contains only the precursors of embryonic mesoderm. This suggests that there has been a progressive displacement of the midstreak mesoderm to extraembryonic sites, which is reminiscent of that occurring in the overlying endodermal tissue. The regionalisation of cell fate in the late-primitive-streak mesoderm bears the same spatial relationship as their ancestors in the epiblast prior to cell ingression. This implies that both the position of the cells in the proximal-distal axis and their proximity to the primitive streak are major determinants for the patterning of the embryonic mesoderm. © 1995 Wiley-Liss, Inc.
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  • 92
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    Developmental Genetics 17 (1995), S. 65-77 
    ISSN: 0192-253X
    Keywords: Follistatin ; activin ; inhibin ; chick ; rhombomeres ; somites ; resegmentation ; neural induction ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Follistatin, a secreted glycoprotein, has been shown to act as a potent neural inducer during early amphibian development. The function of this protein during embryogenesis in higher vertebrates is unclear, and to further our understanding of its role we have cloned, sequenced, and performed an in-depth expressional analysis of the chick homologue of follistatin. In addition we also describe the expression pattern of activin βA and activin β B, proteins that have previously been shown to be able to interact with follistatin. In this study we show that the expression of follistatin and the activins do not always overlap. Follistatin was first detected in Hensen's node and subsequently in the region described by Spratt [1952] as the neuralising area. In older embryos it was also expressed in a highly dynamic manner in the hind-brain as well as in the somites. We also present evidence that follistatin may have a later role in the resegmentation of the somites. We were unable to detect the expression of activin βA during early embryogenesis, whereas activin βB was first expressed in the extending primitive streak and subsequently in the neural folds. The results from this study are consistent with a role for follistatin in neural induction but suggest it has additional functions unrelated to its inhibitory actions on activins. © 1995 Wiley-Liss, Inc.
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  • 93
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    Developmental Genetics 17 (1995), S. 107-116 
    ISSN: 0192-253X
    Keywords: Axis formation ; fork head ; gastrulation ; neurulation ; Xenopus ; zebrafish ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Intense efforts have been devoted to the identification of genes that are causatively involved in pattern-forming events of invertebrates and vertebrates. Several gene families involved in this process have been identified. Here we focus on the Xenopus fork head domain gene family. One of its members, XFKHl/Pintallavis/XFD1, has been shown previously to be involved in axial formation, and the expression patterns of the other family members discussed below suggest that they too play a major role in the initial steps of patterning and axial organization. In this report, we describe four Xenopus fork head genes XFKH3, 4, 5, and 6) and analyze the distribution of their transcripts during early development. XFKH3 is expressed in developing somites but not notochord, XFKH4 in forebrain, anterior retina, and neural crest cells, and XFKH5 in a subset of epidermal cells and the neural floor plate. Finally, transcripts of XFKH6 are seen in neural crest-derived cranial ganglia. In addition, we show that at least some of the zebrafish fork head genes might serve a comparable function. Zebrafish zf-FKHl has a similar expression pattern as Xenopus XFKHl/Pintallavis/XFDl. It is transcribed in the notochord and neural floor plate. The polster or “pillow” also shows very high levels of zf-FKHl mRNA. © 1995 Wiley-Liss, Inc.
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  • 94
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    Developmental Genetics 17 (1995), S. 167-175 
    ISSN: 0192-253X
    Keywords: Mammary gland ; fatty acid binding protein ; mammary derived growth inhibitor ; proliferation ; differentiation ; transgenic mice ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Cardiac fatty acid binding protein (cFABP) is abundantly expressed in the nondividing, functionally differentiated mammary ephithelium. It is very closely related, if not identical to, a previously described protein termed mammary derived growth inhibitor (MDGI). In vitro studies suggest that low concentrations of diffusible cFABP/MDGI may play a hormone-like role in limiting proliferative activity and promoting functional differentiation of this tissue, but no in vivo data to support this idea have been published. To test this hypothesis, we compared the levels of cFABP mRNA with both the epithelial DNA labelling index and levels of β-casein mRNA in wild-type mice. We also investigated the effect of a precocious experimental increase of cFABP levels in the mammary gland of transgenic mice on the labelling index and β-casein mRNA levels. This was accomplished by expressing a bovine cFABP cDNA under the control of the ovine β-lactoglobulin (BLG) gene promoter. We found that although both the DNA labelling index, β-casein mRNA levels, and cFABP mRNA levels in wild-type mice are developmentally regulated, they do not correlate with each other during early pregnancy in individual mice. Moreover, a three- to fourfold increase of total cFABP mRNA in two transgenic lines did not affect the DNA labelling index or the levels of β-casein mRNA, an established marker of differentiation of the mammary epithelium, at this developmental stage. These data suggest that epithelial DNA synthesis, β-casein gene expression, and expression of the cFABP gene are regulated independently in the proliferatively active mammary gland and that the rapidly dividing mammary epithelial cells are not susceptible to the action of cFABP during early pregnancy. © 1995 Wiley-Liss, Inc.
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  • 95
    Electronic Resource
    Electronic Resource
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 17 (1995), S. 223-232 
    ISSN: 0192-253X
    Keywords: Genomic imprinting ; parthenogenetic embryos ; biallelic expression ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Uniparental embryos have been instrumental in studying imprinting because contributions from the parental genomes can be determined unambiguously. In this study, we set out to identify imprinted genes showing differential expression between parthenogenetic and fertilized embryos during preimplantation and early postimplantation stages of development. We identified three genes-apolipoprotein E, pyruvate kinase-3, and protein phosphatase 1 gamma-that represent excellent candidates for imprinted genes, based on the results of the differential screen, their function in differentiation and the cell cycle, and their location within imprinted chromosomal regions. In addition, two novel genes expressed in trophoblast were identified, 1661 and RA81. These genes, together with four known imprinted genes, H19, Igf2r, Igf2, and Snrpn, showed evidence of expression from both parental alleles in early stage embryos, indicating a role for postfertilization processes in regulating imprinted gene function. © 1995 Wiley-Liss, Inc.
    Additional Material: 4 Ill.
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  • 96
    Electronic Resource
    Electronic Resource
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 17 (1995), S. 233-239 
    ISSN: 0192-253X
    Keywords: Genome imprinting ; zebrafish ; Danio rerio ; methylation ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: We have examined the inheritance of a transgene locus in the zebrafish, Daniorerio and demonstrated that its methylation is af fected by the sex of the parent contributing the allele. This parent-of-origin effect on the zebrafish transgene appears to be identical to imprinting as seen in mammals except that in zebrafish, passage of the locus through a female tended to decreased its methylation, whereas passage through a male increased it. Methylation of the transgene in gametic tissues differed from somatic tissue with the locus being hypomethylated in sperm and hypermethylated in the unfertilized egg. The potential identification of imprinting in the zebrafish has important ramifications with respect to the evolution of the process as well as for understanding the role of imprinting in mammals. © 1995 Wiley-Liss, Inc.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
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  • 97
    Electronic Resource
    Electronic Resource
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 17 (1995), S. 272-279 
    ISSN: 0192-253X
    Keywords: Gene amplification ; hypotrichous ciliates ; macronuclear genes ; gene expression ; recognition factors ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: In hypotrich ciliates, the entire silent chromosomal genome of the germinal nucleus (micronucleus) undergoes extensive DNA rearrangements that, during the development of the somatic nucleus (macronucleus) at the beginning a new cell life cycle, eventually result in the production of linear DNA molecules. These molecules represent functional genes, each one consisting of a central coding region flanked by two shorter regions, which apparently lack canonical elements for regulation of replication and transcription. These are amplified to thousands of copies in the “adult” macronucleus of the vegetative cell. We defined the extent of this amplification for allelic codominant genes which, in the macronucleus of Euplotes raikovi, encode polypeptide cell recognition factors (pheromones). This amplification was shown to be allele-specific. The copy numbers of genes coding for pheromones Er-1, Er-2, and Er-10 were determined to be 2.5 - 2.9 × 104, 0.9 - 1.2 × 104, 1.6 - 1.85 × 104 respectively, and these numbers did not appreciably vary during the vegetative cell proliferation. This differential amplification of pheromone genes was (i) independent of whether two genes coexisted in the same heterozy-gous cell or were separated in the corresponding homozygotes, and (ii) directly correlated with quantitative variations in mRNA synthesis and pheromone secretion. On the basis of these results, it is suggested that a mechanism of gene-specific amplification may be used by hypotrich ciliates to modulate gene expression. © 1995 Wiley-Liss, Inc.
    Additional Material: 4 Ill.
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  • 98
    Electronic Resource
    Electronic Resource
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 17 (1995), S. 253-262 
    ISSN: 0192-253X
    Keywords: IGF2 ; IDDM2 ; IDDM ; immune function ; IGFs ; apoptosis ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Parental genomic imprinting is the phenomenon in which the behavior of a gene is modified, depending on the sex of the transmitting parent [Peterson and Sapienza (1993): Annu Rev Genet 27:7-31]. Recent observations have revealed that the inheritance patterns, age-of-onset, severity, and etiology of certain human diseases can be explained by aberrations in the establishment or the maintenance of the imprint. Examples include the Prader-Willi, Angelman, and Beckwith-Wiedemann syndromes [Nicholls (1994): Am J Hum Genet 54:733-740], malignancy [Sapienza (1990): Biochim Biophys Acta 1072:51-61; Feinberg (1993): Nat Genet 4:110-113], and insulin-ependent diabetes mellitus (IDDM) [Julier et al. (1994) Nature 354:155-159; Bennett et al. (1995) Nat Genet 9:284-292]. We review the evidence that implicates an imprinted gene in the INS-IGF2 region of chromosome llp15 in the etiology of IDDM (referred to as the IDDM2 locus) and show that in human fetal pancreas, INS is not imprinted, thus providing an argument against INS as the candidate gene. We also examine imprinting effects on the expression of IGF2 in components of the human immune system believed to be important in IDDM and show imprinted expression in fetal thymus as early as 15 weeks gestation. We demonstrate further that in the circulating mononuclear cells of two individuals, lectin-stimulated IGF2 transcription was biallelic, indicating relaxation of imprinting, whereas in one individual, transcription was monoallelic. Finally, we review the current available data supporting a role for insulin-like growth factor-ll (IGF-II) in the immune system and, more specifically, discuss the evidence supporting a role for the IGFs in the prevention of apoptosis. These data have led us to formulate a novel hypothesis that could mechanistically explain the involvement of the IDDM2 locus in the pathogenesis of IDDM. © 1995 Wiley-Liss, Inc.
    Additional Material: 4 Ill.
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  • 99
    ISSN: 0192-253X
    Keywords: Translation elongation factor 1α (EF-1α) ; developmental regulation ; oogenesis ; microinjection ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Transcription of the Xenopus laevis EF-1αS gene commences at the mid-blastula stage of embryonic development and then continues constitutively in all somatic tissues. The EF-1αS promoter is extremely active in the early Xenopus embryo where EF-1αS transcripts account for as much as 40% of all new polyadenylated transcripts. We have isolated the Xenopus EF-1αS gene and used microinjection techniques to identify promoter elements responsible for embryonic transcription. These in vivo expression studies have identified an enhancer fragment, located approximately 4.4 kb upstream of the transcription start site, that is required for maximum expression from the EF-1αS promoter. The enhancer fragment contains both an octamer and a G/C box sequence, but mutation studies indicate that the octamer plays no significant role in regulation of EF-1αS expression in the embryo. The presence of a G/C element in the enhancer and of multiple G/C boxes in the proximal promoter region suggests that the G/C box binding protein, Spl, plays a major role in the developmental regulation of EF-1αS promoter activity. © 1995 Wiley-Liss, Inc.
    Additional Material: 9 Ill.
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  • 100
    ISSN: 0192-253X
    Keywords: Ecdysone receptor ; Choristoneura fumiferana cDNA cloning ; developmental expression ; molting and metamorphosis ; ecdysone response element ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Degenerate oligonucleotides were designed on the basis of conserved amino acid sequences in the DNA and ligand-binding regions of the members of the steroid hormone receptor superfamily. Using these oligonucleotides in RNA-PCR, a cDNA fragment was isolated from the spruce budworm, Choristoneura fumiferana. Comparison of the deduced amino acid sequence of this cDNA fragment with the members of the steroid hormone receptor superfamily suggested that this PCR fragment is a region of the ecdysone receptor from C. fumiferana. Using this cDNA fragment as a probe, 10 clones were isolated from a cDNA library that was constructed using the RNA from 4- and 5-day old embryos of C. fumiferana. Two cDNA clones (1.3 and 3 kb) that overlap and show amino acid identity with Drosophila melanogaster ecdysone receptor B-1 isoform (DmEcR) were characterized and sequenced. The longest open reading frame had 539 codons and covered the complete EcR coding region. The deduced amino acid sequence of this open reading frame had all five of the regions typical for a steroid hormone nuclear receptor. The C domain or DNA binding region showed the highest identity with EcR proteins from D. melanogaster, Chironomus tentans, Aedes aegypti, Manduca sexta and Bombyx mori. The A/B region, D domain or hinge region, E domain, or ligand binding region also showed significant amino acid similarity with the EcR proteins from the five insects mentioned above. The C. fumiferana ecdysteroid receptor (CfEcR) cDNA probe detected a 6.0-kb mRNA that was present throughout the development of C. fumiferana. The CfEcR mRNA increases in abundance at the time of the ecdysteroid peak during the molting phase in the embryonic, larval and pupal stages but remains low during the intermolt period. In the 6th instar larvae, the 6-kb CfEcR mRNA was detected in the epidermis, fat body, and midgut and maximum expression was observed during the prepupal peak of ecdysteroids in the hemolymph. CfEcR mRNA was induced in ecdysone treated CF-203 cells as well as in the epidermis and midgut of larvae that were fed the nonsteroidal ecdysteroid agonist, RH-5992. The induction occurred within an hour and reached maximum levels around 3 hr, after which it decreased to the basal level by 6 hr. In vitro transcription and translation of the CfEcR cDNA yielded a 67-Kda protein that bound to the ecdysone response element (EcRE) as a heterodimer, along with the ultraspiracle protein.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
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