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  • 1990-1994  (878)
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  • 2015-2019
  • 2010-2014
  • 1990-1994  (878)
  • 1975-1979  (502)
  • 1890-1899
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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Diseases of the colon & rectum 33 (1990), S. 773-777 
    ISSN: 1530-0358
    Keywords: Giant inflammatory polyp ; Idiopathic inflammatory bowel disease ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Five cases of giant inflammatory polyps associated with idiopathic inflammatory bowel disease are reported. Polyps produced intestinal obstruction in three cases; consequently, surgery was performed. In a further two cases, intestinal bleeding was improved by endoscopic polypectomy. Electron microscopy showed fibroblasts, myofibroblasts, mast cells, lymphocytes, collagen fibers, capillaries, and venules. Remnants of the original mucosal epithelial cells, smooth muscle cells, and hypertrophic autonomous nerve plexuses were noted. Nerve fibers were interwoven with the matrix of the polyps. Mast cells were closely linked with vessels, nerves, and collagen fibers. They may have an important role in the excessive granulation, angiogenesis, and fibrotic process in giant inflammatory polyps.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Rheumatology international 10 (1990), S. 103-106 
    ISSN: 1437-160X
    Keywords: Synovium ; Synoviocytes ; Monoclonal antibody ; Immunohistochemistry ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The functionally important lining cells of the synovium (types A and B synoviocytes) are the subjects of much study but have presented problems with their characterization and microscopical identification, particularly at the light level. Type A (macrophage-like) synoviocytes, however are more easily localized than the type B (fibroblast-like) variety because of the greater availability of antimacrophage antisera. We describe, using light and electron microscopy, a monoclonal antibody which in the synovial intimal layer is specific for type B synoviocytes.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Journal of insect behavior 3 (1990), S. 579-587 
    ISSN: 1572-8889
    Keywords: Genetics ; polymorphism ; reproductive isolation ; hovering behavior ; Tabanus nigrovittatus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The salt marsh horse fly, Tabanus nigrovittatusMacquart, exhibits two nonoverlapping daily periods of hovering and mating activity, which are correlated with different environmental temperatures. Allelic and genotypic frequencies of hovering males collected during the two periods were compared by electrophoresis of three polymorphic enzyme loci. Approximately 26% of early-hovering males possessed a Pgmallozyme that was absent in our sample of late-hovering males. However, based on other allozyme loci, we found no evidence for reproductive isolation between early and late hoverers. All the genetic data are consistent with the hypothesis that the Pgmpolymorphism is associated with behaviorally and physiologically distinct groups of males that, by all other criteria, form a single Mendelian population.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Pediatric surgery international 5 (1990), S. 359-360 
    ISSN: 1437-9813
    Keywords: Hirschsprung's disease ; Genetics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Three male children with identical short-segment Hirschsprung's disease born to a young married couple are reported. There was no positive family history despite an extensive search. There were no associated abnormalities. Although sex-modified multifactorial inheritance, with males having a lower threshold of genes for expression of Hirschsprung's disease, is accepted, the identical expression of the disorder in the three siblings presented suggests a dominant, possibly X-linked gene with variable penetrance. Another possibility is that an identical micro-environmental factor was present prenatally resulting in all three boys having Hirschsprung's disease. This is the first report of three siblings with identical short-segment Hirschsprung's disease.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Virchows Archiv 416 (1990), S. 237-248 
    ISSN: 1432-2307
    Keywords: Neurilemoma ; Pseudosarcoma ; Electron microscopy ; Immunohistochemistry ; Cytogenetics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A series of 29 cellular schwannomas is described in terms of their clinical presentation and course, light and electron-microscopic appearance, immunohistochemical properties and cytogenetics. The study indicates that cellular schwannoma can be defined as a subtype of classical schwannoma, characterized by spindle cells forming a compact fascicular, sometimes fibrosarcoma-like growth pattern, a low mitotic activity, a generally moderate nuclear and cellular polymorphism and a high degree of Schwann cell differentiation as seen by electron microscopy and immunohistochemistry. The tumour is characteristically located close to the vertebral column, in the mediastinum or retroperitoneum and has a benign course. Occasionally bone destruction and neurological symptoms develop. The clinical appearance together with the high cellularity, fascicular pattern and mitotic activity had led to the erroneous diagnosis of a soft tissue sarcoma in a few cases, and cellular schwannoma may thus be considered to be a pseudosarcoma. Immunohistochemically, cellular schwannomas appear to deviate from classical schwannomas and malignant peripheral nerve sheath tumours by their expression of glial fibrillary acidic protein. The chromosome analysis revealed a normal diploid stemline karyotype, with a variety of abnormal clones, including one with monosomy 22.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Virchows Archiv 417 (1990), S. 261-266 
    ISSN: 1432-2307
    Keywords: Stomach ; Paraganglioma ; Immunohistochemistry ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary We report the second case of a malignant paraganglioma of the stomach in a 56-year-old female patient. However, our case is the first investigated by immunohistochemistry and electron-microscopy. The tumour was characterized immunohistochemically by the presence of neurofilament protein, glial fibrillary acidic protein, S-100 protein, neuron-specific enolase, chromogranin A, ACTH, leu-enkephalin and vasoactive intestinal polypeptide, and ultrastructurally by demonstration of neurosecretory granules and paranuclear intermediate filament whorls. Despite massive metastatic spread in the abdominal cavity, the patient is still alive 4 years after initial diagnosis.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1432-2307
    Keywords: Mouse ; Nephrotic syndrome ; Hereditary nephritis ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Glomerular lesions in hereditary nephrotic mice (ICGN strain) were investigated by electron microscopy. The glomeruli of unaffected animals, which appeared normal by light microscopy, had developed an ultrastructural change in the glomerular capillary basement membrane (GCBM). There was a partial thickening of the GCBM with bilaminar splitting of the lamina densa and an electron-dense fibrillar material exhibiting cross-striations. In affected animals, light microscopy revealed a marked thickening of GCBM and an increase of mesangial matrix without cellular proliferaton. By electron microscopy, multilaminar splitting of the lamina densa in the thickened GCBMs and fusion of the epithelial foot processes were observed. In some severely affected animals, immune complex deposition was found in GCBM, but little if any was observed in other animals. In the end, the glomeruli were globally sclerosed. Our findings suggest that initial structural abnormalities in GCBM may play an important role in the onset and development of the disease, though subsequent events such as immune complex deposition would modify the disease.
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1432-2307
    Keywords: Cyclosporin A ; Human skin xenografts ; Histology ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Cyclosporin A (CsA) is a potent immunosuppressant with a selective activity on T-helper lymphocytes. However, CsA also exerts biological effects on non-lymphoid cells (fibroblasts, endothelial and epithelial cells). CsA can inhibit in vivo and in vitro DNA synthesis of epidermal keratinocytes (EK) and induces in vivo morphological alterations of kidney epithelial cells. In the present study we investigated the in vivo effects of a short-term CsA treatment (50 mg/kg per day) on DNA synthesis (evaluated through 5-bromo-2′-deoxyuridine incorporation) and on the histological features of normal human skin xenografted (NHSX) on to congenitally athymic nude mice. When compared with control NHSX, CsA induced a statistically significant inhibition of DNA synthesis of NHSX EK. At the light- and electron-microscopic level, apart from a decrease in the thickness of the viable epidermis of NHSX (statistically non-significant), no noticeable differences between treated and control NHSX could be detected. EK, Langerhans cells and melanocytes appeared morphologically unaffected by CsA and no signs of acute toxicity (giant mitochondria, vacuolization, microcalcifications) were seen. These results suggest that CsA exerts a subtle effect on human EK; indeed, despite an unequivocal antiproliferative activity, no significant histological changes related to the acute CsA toxicity seem to be induced on the various epidermal cell types.
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 1432-2307
    Keywords: Salivary gland ; Neoplasm ; Mucoepidermoid carcinoma ; Electron microscopy ; Histogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Current classifications of salivary gland tumors separate mucoepidermoid carcinoma from other neoplasms on the basis of a number of histological features, in particular the lack of participation of neoplastic myoepithelial cells. However, ultrastructural examination of low- and intermediate-grade mucoepidermoid carcinomas and pleomorphic adenomas reveals many common organizational and cellular features. Of prime importance is the relationship of intermediate cells to the luminal cells in mucoepidermoid carcinomas, which is remarkably similar to that seen between modified myoepithelial cells and luminal cells in pleomorphic adenomas. The results suggest that intermediate cells of mucoepidermoid carcinoma are the counterpart of the modified myoepithelial cells of pleomorphic adenoma. The generally accepted hypothesis that the former tumor develops from an excretory duct reserve cell, while the latter originates from an intercalated duct stem cell does not seem to be valid; pleomorphic adenoma and mucoepidermoid carcinoma appear to be closely related morphologically.
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 1432-2307
    Keywords: Atubular glomeruli ; Chronic nephropathy ; Electron microscopy ; Lithium ; Stereology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The very heterogeneous population of glomeruli in rats with lithium-induced chronic nephropathy which includes small glomeruli without connection to a proximal tubule (atubular glomeruli) and large hypertropic glomeruli with connection to a normal proximal tubule, was studied at the ultrastructural level, using stereological methods. After 8 weeks of lithium treatment followed by 8 weeks without lithium the hypertrophic glomeruli showed no changes in their relative ultrastructural composition, including normal mesangium, basement membrane-like material and peripheral basement membrane. The absolute quantities of each component were, however, increased due to the increased volume of the glomeruli. The atubular glomeruli had increased volume fractions of mesangium, peripheral basement membrane, basement membrane-like material and epithelium, whereas the absolute quantities were decreased due to the decreased volume. The thickness of the basement membrane was within normal limits in the group of hypertrophic glomeruli but increased by 31% above controls in the group of atubular glomeruli. Both groups of glomeruli in lithium-treated animals showed normal mean foot process width, but with a slightly abnormal distribution. The atubular glomeruli showed a disproportionate large decrease in peripheral filtration surface and capillary length, compared with the reduction in glomerular volume, whereas the hypertrophic glomeruli showed changes in proportion with the increased volume.
    Type of Medium: Electronic Resource
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  • 11
    ISSN: 1432-0568
    Keywords: Neuropeptide Y ; Substance P ; Immunocytochemistry ; C-fibers ; Dura mater ; Dural sinus ; Meningeal arteries ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Density and pattern of nerve fibers with neuropeptide Y-like immunoreactivity (NPY-LI) and substance P-like immunoreactivity (SP-LI) in the rat dura mater encephali were investigated by light and electron microscopy using whole-mount preparations. NPY-LI fibers are observed throughout the encephalic dura mater. A remarkable net of NPY-LI nerve fibers is located in the walls of the sagittal and transverse sinuses. Beyond that NPY-LI network, distinct NPY-LI nerve fibers or plexus occur in the rostral falx, parietal dura mater of the olfactory bulb, supratentorial dura mater, parietal dura mater of the cerebellum, tentorium cerebelli and the ventral dura mater. Electron microscopic studies reveal that NPY-LI is exclusively located in unmyelinated axons of small and large nerve fiber bundles, with or without a perineural sheath. Immunopositive C-fibers are predominantly associated with the vascular bed. SP-LI nerve fibers have a moderate and more uniform distribution in the encephalic dura mater. A distinct plexus of SP-LI fibers follows the branches of the middle meningeal artery and the adjacent dura mater. SP-LI fibers are most prominent in the parietal dura mater of the cerebellum. Fine beaded SP-LI fibers, arising from larger SP-LI fiber bundles, are observed in close association to the capillary bed. SP-LI axons are all unmyelinated. They are found in larger nerve fiber bundles with a perineural sheath or in Schwann cells lacking any perineural sheath. The function of NPY-LI and SP-LI nerve fibers in the rat dura mater is discussed in relation to their topography, density and termination.
    Type of Medium: Electronic Resource
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  • 12
    Electronic Resource
    Electronic Resource
    Springer
    Acta neuropathologica 79 (1990), S. 387-394 
    ISSN: 1432-0533
    Keywords: Triosephophosphate isomerase (TPI) ; Mitochondrial myopathy ; Muscle tissue ; Electron microscopy ; Enzyme histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Morphological changes are shown in the muscle biopsy specimens of an 8-year-old girl who suffered from a triosephosphate isomerase (TPI) deficiency, resulting in a chronic, nonspherocytic, hemolytic anemia, mental retardation and neuromuscular impairment. The newly introduced enzyme histochemical reaction for TPI demonstrated a total lack of histochemically detectable enzyme activity, whereas biochemical analysis of muscle tissue revealed less than 10% of the normal enzyme activity. Electron microscopy showed a degenerative myopathy with an increase in the amount of intracellular glycogen. Additionally, mitochondrial changes within the muscle fibers were observed to be similar to those in mitochondrial myopathies. The disturbed balance between glycerinaldehyde phosphate and dihydroxy-acetone phosphate, due to the deficiency of the TPI enzyme, is interpreted as the biochemical background of an impaired electron transport across the mitochondrial membrane, resulting in the coexistence of an impaired glycolytic pathway and an impaired mitochondrial metabolism of muscle cells.
    Type of Medium: Electronic Resource
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  • 13
    Electronic Resource
    Electronic Resource
    Springer
    Acta neuropathologica 80 (1990), S. 123-128 
    ISSN: 1432-0533
    Keywords: Marinesco-Sjögren syndrome ; Muscle biopsy ; Electron microscopy ; Autophagocytosis ; Double-membrane structure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Seven muscle biopsies from patients with the clinical characteristics of Marinesco-Sjögren syndrome (MSS) revealed myopathic changes of two types; muscle fiber necrosis followed by regeneration and focal myofibrillar degeneration inducing autophagocytosis with rimmed vacuole formation. In two young patients, massive muscle fiber necrosis with phagocytic invasion was the predominant feature and autophagic phenomenon was minimal, resembling the findings in progressive muscular dystrophy. Myofibrillar degeneration with autophagic phenomenon was prominent in five adult patients. The coexistence of these two degenerative processes and the secondarily induced reactive changes of muscle fiber hypertrophy, interstitial fibrosis, occasional ragged-red fibers and type 1 fiber predominance, are responsible for the wide spectrum of muscle pathology in MSS. The dense double-membrane structure surrounding myonuclei, previously reported as being specific to MSS, was present in only one biopsy.
    Type of Medium: Electronic Resource
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  • 14
    ISSN: 1432-0533
    Keywords: Global ischemia ; Cerebellum ; Hippocampus ; Electron microscopy ; Synapse
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary We have developed a functional vegetative model by an 18-min clamping of the ascending aorta combined with a bypass formation between the aorta to right atrium and the aorta to femoral vein. Complete global brain ischemia (CGBI) induced for 18 min with this model provided the following distinct advantages: cardiopulmonary functions were well preserved during postischemic recirculation, and all dogs survived without serious extracerebral complications. Neuronal damage in vegetative dog induced by an 18-min CGBI was studied by light and electron microscopy. The Purkinje cells and the hippocampal CA1 pyramidal cells showing clumping of nuclear chromatin and slightly increased stainability were observed after CGBI without recirculation. All these neurons showed transient increased stainability with microvacuolation 15 min after recirculation. Over 50% of these neurons showed virtually normal features 1 h after recirculation. Damage to these neurons progressed again slowly up to 6 h after recirculation. However, all these neurons had disintegrated 2–3 days after recirculation. A decrease in synaptic vesicles was observed in many presynaptic terminals in the molecular layers of the cerebellum after CGBI without recirculation. These changes in the presynaptic terminals progressed 15 min after recirculation. These results indicated that the damage to the Purkinje cells and the CA1 pyramidal cells induced by CGBI consisted of two phases, and that the change in the early phase was reversible. We speculate that the damage to the Purkinje cells in the early stage is related to the decrease of the synaptic vesicles in the presynaptic terminals.
    Type of Medium: Electronic Resource
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  • 15
    Electronic Resource
    Electronic Resource
    Springer
    Acta neuropathologica 81 (1990), S. 162-177 
    ISSN: 1432-0533
    Keywords: Infusion edema ; Immunocytochemistry ; Clearance ; Electron microscopy ; Brain edema
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The pathways and mechanisms by which edematous fluid accumulation in the extracellular space (ECS) clears from brain are poorly understood. The objective of this study was to explore, using immunocytochemical technique, the fate of a proteinaceous fluid added to the brain ECS and to study the clearance pathways. The protein movement of this edema fluid was investigated using the direct infusion model on rats. Rat albumin (20 μl) was slowly infused into the caudate-putamen of anesthetized adult rats and the spread and clearance of the edema was followed in various brain regions using immunocytochemical and conventional light and electron microscopy at 0, 1, 2, 3, 4, 6, and 8 days post-infusion. Our studies showed that protein-rich edema fluid cleared slowly from the brain, with 8 days required for the infusion albumin to exit completely from the brain parenchyma. Immediately following infusion, the albumin was distributed in the ECS of the white matter and the overlying deep cortical layers related to the infusion site. During the next 24 h, more of the infused albumin traveled through the ECS to the cortical surface where the albumin passed through the glia limitans to reach the subarachnoid front. Additionally, at 48 h post-infusion, that albumin, which had migrated to the ventricular wall, cleared from the ECS of the subependymal white matter and the ependymal clefts to reach the ventricular cerebrospinal fluid (CSF). In edematous regions, the perivascular spaces of venules and veins were filled with reaction product. Continuity of this perivascular reaction product existed from the deep edematous area to the temporobasal subarachnoid space from where the reaction product gradually disappeared from the parenchyma. From these studies we infer that during the late state of the resolution process the edema front moves toward both the ventricle and the cortical surface to reach the CSF. Thus, among the potential routes for edema clearance, the pathways leading to CSF clearance of fluid predominated. During this clearance process, neither neurons, glia nor the vascular endothelium showed any endocytotic response to the infused albumin throughout the 8-day course. We conclude from these observations that the CSF pathway is the major route of protein-rich edema clearance, when such clearance is not complicated by any concomitant CNS perturbation.
    Type of Medium: Electronic Resource
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  • 16
    Electronic Resource
    Electronic Resource
    Springer
    Anatomy and embryology 182 (1990), S. 539-545 
    ISSN: 1432-0568
    Keywords: Corticotrophs ; Pituitary gland ; Golden hamster ; Immunocytochemistry ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The ultrastructure of corticotrophs in the pituitary of golden hamsters was studied by immunocytochemistry. Corticotrophs were classified into three types according to the different size of the secretory granules. Type A and B cells were oval or polygonal in shape containing small (158±38 nm) and medium-sized (250±53 nm) secretory granules, respectively. Type C cells were usually pyramidal or irregular in shape, and contained large secretory granules (380±78 nm). The cytoplasmic organelles of type B and C cells were fairly well developed. In all types of corticotrophs, the secretory granules varied in electron density, and were either arranged in a single row along the cell membrane, or concentrated in the vascular pole of the cytoplasm. Many of the corticotrophs sent processes to encircle neighboring somatotrophs. In the female, the proportions of type A and B cells were higher then those in the male, whereas that of the type C cells was lower. All three types of corticotrophs were observed in the early postnatal stage. The population of type A cells decreased with the advancement of postnatal development, with a concomitant increase of that of the type C cells. Thus, type A cells might represent the immature type of corticotrophs which would evolve to become the type C cells. One to 2 weeks after adrenalectomy, the number of type C cells was drastically increased. This was accompanied by their prominent changes in ultrastructure as in the type B cells. The cytoplasm became hypertrophic with extensive dilated cisternae of rough endoplasmic reticulum. The large Golgi apparatus displayed profiles seemingly involved in the formation of secretory granules. The number of type A cells was moderately decreased, and they showed little morphological alteration. Since type B and C corticotrophs exhibited a remarkable response to adrenalectomy, it is concluded that they represent the active state of corticotrophs.
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  • 17
    Electronic Resource
    Electronic Resource
    Springer
    Acta neuropathologica 79 (1990), S. 473-479 
    ISSN: 1432-0533
    Keywords: Central neurocytoma ; Neuronal differentiation ; Glial differentiation ; Synaptophysin ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Central neurocytoma has been characterised by its intraventricular localisation, predominant occurrence in young adults, oligodendroglioma-like histology, benign course and ultrastructural evidence for neuronal differentiation. Eleven intraventricular central neurocytomas were studied histopathologically, employing cell type-specific immunocytochemical markers and electron microscopic analysis. In the past, these lesions have caused diagnostic problems since central neurocytomas share basic histopathological features with other periventricular neoplasms. Accordingly, several tumours of this series had previously been classified as ependymomas of the foramen of Monro or oligodendrogliomas. Although generally regarded as benign lesions, two central neurocytomas of this series showed histopathological evidence of anaplasia, with focal necrosis, mitotic activity and vascular proliferation. All central neurocytomas exhibited immunoreactivity for neuronspecific enolase and synaptophysin, indicating consistent neuronal differentiation. Three tumours were studied by electron microscopy and contained synaptic vesicles, neuritic processes and neurosecretory granules. In addition, one tumour contained ganglioid cells and this was associated with focal immunoreactivity for neurofilament protein, suggesting that some central neurocytomas may, at least focally, continue to differentiate towards the formation of mature neurons. Two of the tumours expressed glial fibrillary acidic protein in a considerable percentage of neoplastic cells which demonstrates a capacity for bipotential, i.e. glial and neuronal differentiation. We conclude that the central neurocytoma can be reliably diagnosed using antibodies to neuron-specific enolase and synaptophysin, and that histogenetically, this neoplasm is derived from a neuroectodermal precursor cell capable of both, neuronal and glial differentiation. The hypothesis is proposed that the central neurocytoma originates from the subependymal plate of the lateral ventricles, an embryonal matrix cell layer which postnatally maintains a limited proliferative potential.
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  • 18
    Electronic Resource
    Electronic Resource
    Springer
    Acta neuropathologica 81 (1990), S. 228-232 
    ISSN: 1432-0533
    Keywords: Cerebral tumor ; Polar spongioblastoma ; Astrocytoma ; Immunohistochemistry ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A case is reported of a 9-year-old boy with a cerebral polar spongioblastoma. This neoplasm, first described by Russell and Cairns in 1947, is morphologically a distinct entity characterized by bipolar tumor cells with palisading nuclei. In the case under study immunoreactivity for neuron-specific enolase was found and ultrastructural features of developing neuronal elements were present. A neuro-endocrine nature was suggested by de Chadarévian et al. (1984) in a morphologically similar case. These findings are in contrast with the longheld view that the polar spongioblastoma is cytogenetically related to the embryonal radial glial cells.
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  • 19
    ISSN: 1432-0533
    Keywords: Creutzfeldt-Jakob disease ; Electron microscopy ; Scrapie ; Tubulovesicular structures
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary We have consistently observed tubulovesicular structures in brain tissues during the terminal stages of naturally occurring and experimentally induced spongiform encephalopathies, irrespective of the host species and virus strain. In NIH Swiss mice inoculated intracerebrally or intraocularly with the Fujisaki strain of Creutzfeldt-Jakob disease (CJD) virus, tubulovesicular structures, measuring 20–50 nm in diameter, were particularly prominent in dilated, pre-and postsynaptic neuronal processes, occasionally being mixed with synaptic vesicles. These structures appeared 13 weeks following intracerebral inoculation, 5 weeks before the onset of clinical signs, when spongiform changes were also detected. The number and density of tubulovesicular structures increased steadily during the course of clinical disease, and were particularly abundant in mice 47 to 51 weeks after intraocular inoculation. In hamsters infected with the 263 K strain of scrapie virus, these structures were initially detected 3 weeks following intracerebral inoculation and increased dramatically at 10 weeks postinoculation. The appearance of tubulovesicular structures before the onset of overt disease in mice inoculated with CJD virus by either the intracerebral or intraocular route, and before the appearance of other neuropathological changes in hamsters infected with scrapie virus, indicate that they represent either a part or aggregate of the infectious virus or a pathological product of the infection.
    Type of Medium: Electronic Resource
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  • 20
    ISSN: 1432-0533
    Keywords: Brain cell culture ; Canine distemper virus ; Cerebroside sulfotransferase ; Electron microscopy ; Oligodendrocytes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary To study the pathomechanism of demyelination in canine distemper (CD), dog brain cell cultures were infected with virulent A75/17-CD virus (CDV) and examined ultrastructurally. Special attention was paid to the oligodendrocytes, which were specifically immunolabelled. In addition, cerebroside sulfotransferase (CST), an enzyme specific for oligodendrocyte activity was assayed during the course of the infection. Infection and maturation as well as CDV-induced changes were found in astrocytes and brain macrophages. Infection of oligodendrocytes was rarely seen, although CST activity of the culture markedly decreased and vacuolar degeneration of these cells occurred, resulting in their complete disappearance. We concluded that the degeneration of oligodendrocytes and demyelination is not due to direct virus-oligodendrocyte interaction, but due to CDV-induced events in other glial cells.
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  • 21
    ISSN: 1432-0533
    Keywords: Composite ganglioneuroblastoma ; Electron microscopy ; Cerebrum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary An unusual cerebral tumor is reported in a 14-year-old boy. On light and electron microscopy, the constituent cells were very complex; the majority of the neoplastic cells were primitive neuroectodermal cells dispersed in myxomatous or fibrous stroma. Neoplastic neuronal cells and hypertrophic astrocytes were also observed in these areas. The neuronal cells showed a continuous spectrum of differentiation from very primitive to mature ganglion cells. Furthermore, the tumor contained a highly cellular discrete area consisting of neuroblasts and their precursor cells. From these findings, a diagnosis of composite ganglioneuroblastoma was made.
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  • 22
    Electronic Resource
    Electronic Resource
    Springer
    Acta neuropathologica 80 (1990), S. 103-107 
    ISSN: 1432-0533
    Keywords: Brain neoplasms ; Medulloepithelioma ; Primitive neuro-ectodermal tumor ; Immunohistochemistry ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A case is reported of a boy, 3 years of age, with a large medulloepithelioma in the left cerebral hemisphere. Medulloepitheliomas are rare tumors of the primitive medullar epithelium. Histological, immunohistochemical and electron microscopical findings are presented. We discuss previously reported cases, the ontogeny of this type of tumor and the relation to the socalled primitive neuro-ectodermal tumors (PNET).
    Type of Medium: Electronic Resource
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  • 23
    Electronic Resource
    Electronic Resource
    Springer
    Acta neuropathologica 80 (1990), S. 671-679 
    ISSN: 1432-0533
    Keywords: Peroneal muscular atrophy ; Rigidity and tremor ; Autopsy ; Morphometry ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary An autopsy case of hereditary peroneal muscular atrophy (PMA) with rigidity and static tremor is presented. The patient developed slowly progressive distal muscular atrophy of the legs at the age of 15 years. By the age of 52 years, PMA became marked associated with pes cavus, and tremor and rigidity of the extremities were noted. Motor and sensory conduction velocities gradually depressed and lost near the end of his life. At autopsy, the major neuropathological abnormalities involved the peripheral nervous systems, and were characterized by axonal atrophy and loss of myelinated fibres. These changes involved both the proximal and distal nerves, being more severely affected in the distal. The pathological changes in other regions of the nervous systems were mainly confined to the spinal cord, dorsal ganglia and spinal nerve roots, and pigmented neurons in the brain stem. Morphometrically, the total fascicular area was much smaller than in control, but the total number of myelinated fibers greatly outnumbered that of control 75 200 to 48 200 at the proximal sciatic nerve and then gradually decreased towards the periphery; however, even in the distal sural nerve, the total number of myelinated fibers exceeded that of control (6820 to 5469). Thus, the density of myelinated fibers were much higher, being 1.5 to 2 times greater, than in control. Its abrupt decline at the distal nerve might account for neurogenic atrophy of the distal musculature. Unmyelinated fibers were slightly increased in density and not atrophic. This case is unique in its clinicopathology and does not belong to any subtypes of PMA including “neuronal plus”.
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  • 24
    ISSN: 1432-0533
    Keywords: HTLV-I-associated myelopathy ; Spinal cord lesion ; Electron microscopy ; Primary demyelination ; Remyelination by oligodendrocytes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary We describe postmortem findings in a patient with human T lymphotropic virus type I (HTLV-I)-as-sociated myelopathy (HAM). The patient developed the disease 8 years after blood transfusion and showed good response to corticosteroid treatment but died of cardiac failure. Histologically, chronic, mild meningoence-phalomyelitis was noted predominantly involving the bilateral lateral and anterior columns of the middle to lower thoracic segments. The spinal cord lesions showed obvious loss of myelinated nerve fibers and fibrillary gliosis with minimal inflammatory cell infiltration. Electron microscopy of the lesion revealed disintegration of the myelin sheaths, regular separation of the minor dense line of the myelin sheaths, and completely demyelinated axons. In addition, remyelinated fibers with thin central myelin sheaths and disproportionately large axons were seen frequently. These findings indicate that primary demyelination and remyelination by oligodendrocytes occur in the spinal cord lesions of HAM.
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  • 25
    ISSN: 1432-0568
    Keywords: In vitro culture ; Electron microscopy ; Fluorography ; Blastocyst ; Rabbit
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Day-6 rabbit blastocysts were cultured in Ham's F10 medium supplemented with polyvinylpyrrolidone as a macromolecular component, for 4 to 12 h. The integrity of the blastocyst cells was demonstrated by electron microscopy. Expansion and biosynthesis of proteins and of DNA were studied after culturing in the presence of 35S-methionine and 3H-thymidine. Polyvinylpyrrolidone did not interfere with the subsequent protein analysis, which was performed by two dimensional gel electrophoresis followed by silver staining and fluorography. More than 600 labelled proteins were found in the blastocyst tissue, many of them were also present in the blastocyst fluid and in the blastocyst coverings. Several proteins seemed to be produced for incorporation into the blastocyst coverings; others, only detected in the culture medium, might have been synthesized for secretion into the environment.
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  • 26
    ISSN: 1432-0568
    Keywords: Immunocytochemistry ; Electron microscopy ; CR3 receptors ; Amoeboid microglia ; Rats
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The present study described the labelling of amoeboid microglial cells in the postnatal rat brain with OX-42, an antibody that recognizes type 3 complement receptors CR3 in mononuclear phagocytes. Of the diverse morphological forms of amoeboid microglia present in the corpus callosum in early postnatal (2–5 days) rats, cells with a round regular outline, or showing short stout processes, were the most intensely stained. When traced from the main cell colony into the borderline zone with the cortex, the immunoreactivity of amoeboid microglia that assumed a ramified form was drastically reduced. Examination of materials from the late postnatal (8–12 days) age group showed that the majority of the OX-42 positive cells in the corpus callosum became oval, elongated and ramified. Immunoelectron microscopy confirmed the above observations, and also showed that the immunoreactivity in the round amoeboid microglia was localized in their plasma membrane, surface projections and invaginations, as well as in some of the subsurface vacuoles. The immunoreactivity was reduced in the oval cells, and diminished in the elongated or ramified form. It is proposed that the presence of CR3 membrane receptors in amoeboid microglial cells is related to their active role in endocytosis. These, however, diminish with the growth of the brain.
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  • 27
    ISSN: 1432-0568
    Keywords: Astrocyte ; Radial astrocyte ; GFAP ; Electron microscopy ; Phylogeny
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The astroglial cells have been studied in the lizard spinal cord by means of metallic impregnations, immunohistochemical (glial fibrillary acidic protein) and ultrastructural methods. Three astroglial cell types have been immunohistochemically identified: ependymocytes, radial astrocytes and astrocytes. Transitional forms have also been observed. Scarce immunopositive ependymocytes were located in the dorsal and ventral regions of the ependyma. The radial astrocytic somata were located around the ependymal layer and their processes reached the subpial glia limitans. Typical astrocytes were the most abundant astroglial cell type; astrocytes located in the ventral horn showed a greater development than those of the dorsal horn. In the white matter, the astrocytes were large and their processes formed part of the subpial glia limitans; on some occasions, astrocytic cell bodies also formed part of this subpial limitans. Transitional elements between astrocytes and radial astrocytes were observed in both grey and white matter. The perivascular and subpial glia limitans were continuous and showed a strong immunoreactivity. The comparative analysis of our results in the lizard spinal cord with those in other vertebrate groups leads us to conclude that reptiles could represent the key group in the phylogenetic evolution of the astroglial cells in vertebrates.
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  • 28
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    Archives of dermatological research 282 (1990), S. 363-370 
    ISSN: 1432-069X
    Keywords: Autosomal recessive palmoplantar keratosis ; Electron microscopy ; Keratinization ; Keratohyalin ; Keratohyaline proteins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A new kind of diffuse palmoplantar keratoderma with autosomal recessive inheritance and without associated symptoms was described in Norrbotten, Sweden by Gamborg Nielsen in 1985. Clinically, it ranges between the less severe dominant Unna-Thost type and the more severe recessive Meleda type, as it is milder than the latter. Skin biopsies of five patients from three different families with this new palmoplantar keratoderma, as well as five obligatory heterozygotes from one family, were investigated ultrastructurally in order to characterize this new entity and to differentiate it from the Meleda type. Several features are common to both autosomal recessive palmoplantar keratoses. They show a broadened granular layer, a transit region consisting of cells with a marginal envelope, and considerable hyperkeratosis. Morphologically, this transformation delay is less pronounced in the Gamborg Nielsen type than in the classical Meleda type. As is typical for ridged skin, both types of palmoplantar keratoses possess composite keratohyaline granules. In contrast to the normal appearance of keratohyaline granules in the Meleda type, the Gamborg Nielsen type also shows qualitative deviations of keratohyaline granules with different degrees of spongiosity and electron density and sometimes with a granular border. It seems that abnormal keratohyaline proteins are synthesized that behave differently. The sudden transformation of a granular into a horny cell is physiologically regulated by different enzymes. A delay in this process may be caused by a mutation that reduces or alters the enzymes concerned. We assume the palmoplantar keratoderma of the Gamborg Nielsen type to be a variant of the heterogeneous group of the Meleda type of palmoplantar keratoderma with autosomal recessive inheritance.
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  • 29
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    Archives of dermatological research 282 (1990), S. 402-407 
    ISSN: 1432-069X
    Keywords: Electron microscopy ; Culture ; Hair cells ; Growth ; Differentiation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The cultured hair cells from 4-day-old C3H mice were studied by electron microscopy. The hair roots isolated from the skin by collagenase digestion were dispersed into a cell suspension by treatment with a mixture of trypsin and ethylenediaminetetraacetate. The cells were cultured in MCDB-153 (a medium containing seven growth factors) for 1, 3, 6 or 13 days. The number of cultured cells on day 3 was twice that on day 1, and stayed at the same level until day 13. By electron microscopy, some of the cells cultured for 1 day were seen to be undifferentiated and others already showed differentiation into various hair structures. Such differentiated cells disappeared on day 3 and most of the cells cultured for 3 days were undifferentiated. Cells cultured for 6 days were differentiated showing inner root sheath cell, hair cortical cell and medulla cell structures. The characteristics of these cultured cells corresponded well to those of in vivo cells of the hair tissues from the back skin of 7-day-old C3H mice. On day 13 degeneration occurred in the cultured cells. In none of these cultures were mesenchymal cells, such as fibroblasts, found. The present electron microscopic study reveals that immature cells obtained from mouse hair tissues proliferate in vitro and differentiate into several subpopulations corresponding to those of in vivo cell layers of hair tissues. The present culture technique may be useful for studies of hair cell growth and differentiation.
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  • 30
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    Archives of dermatological research 282 (1990), S. 12-16 
    ISSN: 1432-069X
    Keywords: Immunogold ; Electron microscopy ; Eccrine sweat glands ; Keratin ; Fucose
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Human eccrine sweat glands were embedded in Lowicryl K4M. Cytokeratin proteins and blood group H antigen were localized by applying a postembedding immunogold method using a monoclonal antikeratin antibody and the lectin Ulex europaeus I. The antikeratin antibody labeled intermediate filaments in the secretory coil and dermal duct. Within dark secretory cells bundles of filaments criss-crossing the cell were labeled. Within the luminal cells of the dermal duct filaments arranged parallel to the cell surface and lying in the apex of the cell were labeled, too. The association of keratin filaments with desmosomes was visualized demonstrating their subcellular connection with other cell organelles. The desmosomes themselves remained unlabeled. The lectin Ulex europaeus I is a blood group H specific lectin and binds to α-l-fucosyl-containing glycoproteins. Dark cells of the secretory coil reacted with the lectin. Here the secretory granules, the lateral cell membranes, and the microvilli membranes were labeled. The endoplasmatic reticulum, the Golgi complex, and transport vesicles were not labeled, although the glycoprotein synthesis is considered to be located in the Golgi complex. Thus, either the number of α-l-fucose molecules in the Golgi is too low to be detected by the technique employed or the determinant of blood group H antigen is released after the secretory granules and transport vesicles leave the Golgi complex.
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  • 31
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    Archives of dermatological research 282 (1990), S. 71-75 
    ISSN: 1432-069X
    Keywords: Bicomponent keratohyalin ; Ridged skin ; Keratinization ; Sweat ducts ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Up to now, bicomponent keratohyalin has only been described for rat epithelium and human intraepidermal sweat ducts and fetal nail organ cells. In normal human interductal epidermis, the keratohyalin appears homogeneous, osmiophilic and stellate in shape. Under pathological conditions, bicomponent keratohyalin has been observed in different palmoplantar keratoses and has therefore been thought to be associated with abnormal keratosis. We studied the keratinization process in normal human plantar epidermis, in which keratohyalin was found to exhibit several morphological differences as compared to that seen in non-ridged skin. The most striking feature was seen in upper granular cells, where the keratohyalin granules consisted of two components of differing electron density. The electron-dense component formed the main part of the composite granule and was found in the cytoplasm of lower and upper granular cells. The less-electron-dense component was attached to the main component and appeared in the cytoplasm of upper granular cells, forming the convex contact zone. No intranuclear osmiophilic inclusions were present. The respective electron densities of the two keratohyalin components of ridged skin were obviously different to that of the bicomponent keratohyalin granules seen in the epidermal sweat-duct cells of the same specimen. These findings indicate the presence of at least two different types of keratohyalin proteins in normal human ridged skin. They can be distinguished at the electron-microscope level and differ from the keratohyalin of human non-ridged skin as well as from bicomponent keratohyalin granules derived from human epidermal sweat-duct cells or from rat epithelium.
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  • 32
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    Archives of dermatological research 282 (1990), S. 227-233 
    ISSN: 1432-069X
    Keywords: Genodermatoses ; Keratinization disorders ; Fetal cytokeratins ; Immunohistochemistry ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The pathogenesis of a rare form of the ichthyotic diseases, ichthyosis hystrix Curth-Macklin, was investigated by immunohistochemistry and electron microscopy. Monoclonal antibodies (Mabs) against keratins expressed in normal basal cells (PKK2 and KA1), Mabs against keratins only present in normal fetal skin (PKK1), and Mabs against keratins 1, 2, 10, and 11 (KA5 and K8.60) were used. The Mabs reacting with normal basal cells showed an increased reaction with many cell layers. The Mab PKK1 distinctly reacted with the basal cell layer, suggesting an expression of fetal keratins. Electron microscopic study of both normal-looking and involved skin revealed the keratinization disorder characterized by tonofilament shells, perinuclear vacuoles, and binuclear keratinocytes. The results suggest that there is no prematurity of keratinization, but rather a pathological expression of specific keratin genes leading to expression of fetal keratins in this form of ichthyosis hystrix.
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  • 33
    ISSN: 1432-0584
    Keywords: Electron microscopy ; Leukemia ; Minimally differentiated leukemia ; Childhood
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The ultrastructural, light microscopical and immunological features of twelve cases of acute childhood leukemia are described. Nine cases were unclassifiable by light microscopy, morphology and cytochemistry, and three were difficult to classify because of a low percentage of Sudan-Black B positive blasts. By means of electron microscopy (including peroxidase cytochemistry), two main groups were seen: 1. Acute myeloid leukemia, in which could be distinguished a) a more differentiated myeloid leukemia, b) a leukemia with megakaryoblastic involvement and c) a minimally differentiated acute myeloid leukemia with granules present and 2. lymphoblastic leukemia. One case could not be classified. The first group included two possible cases of a hybrid leukemia with CD19 or CD10 positivity as well as ultrastructural peroxidase activity. We conclude that electron microscopy aids to further classification of minimally differentiated and hybrid acute leukemias.
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  • 34
    ISSN: 1432-1106
    Keywords: Hypoglossal nucleus ; Catecholamines ; Norepinephrine ; Immunocytochemistry ; Electron microscopy ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A correlative light and electron microscopic investigation was undertaken to determine the morphology and distribution of catecholamine (CA)-containing axon terminals in the hypoglossal nucleus (XII) of the rat. This was accomplished immunocytochemically with antibody to tyrosine hydroxylase (TH). The major findings in this study were the following: 1) Immunoreactive profiles were found throughout XII and included unmyelinated axons, varicosities, axon terminals and dendrites; 2) Nonsynaptic immunoreactive profiles (preterminal axons, varicosities) were more frequently observed (55.2%) than synaptic profiles (43.5%); 3) CA-containing axon terminals ending on dendrites were more numerous (71.8%) than those synapsing on somata (25.4%) or nonlabeled axon terminals (2.7%); 4) The morphology of labeled axon terminals was variable. Axodendritic terminals typically contained numerous small, round agranular vesicles, a few large dense-core vesicles and were associated with either a symmetric or no synaptic specialization, axosomatic terminals were often associated with a presynaptic membrane thickening or a symmetric synaptic specialization and contained small, round and a few elliptical-shaped vesicles, while axoaxonic synapses formed asymmetric postsynaptic specializations; and 5) CA-positive dendritic processes were identified in XII. These findings confirm the CA innervation of XII, and suggest a complex, multifunctional role for CA in controlling oro-lingual motor behavior.
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  • 35
    ISSN: 1432-198X
    Keywords: Juvenile chronic arthritis ; Renal amyloidosis ; Chlorambucil ; Electron microscopy ; Protein AA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Over a 22-year period, eith patients affected with severe systemic or polyarticular juvenile chronic arthritis (JCA) developed systemic amyloidosis with nephrotic syndrome. They were treated with chlorambucil over 5–192 months (mean=44 months). With treatment, an abrupt decrease in the severity of JCA was observed in six patients but two patients were chlorambucil resistant. After a mean follow-up period of 10 years from onset of renal symptoms, one chlorambucil-resistant patient died of end-stage renal failure; two patients have a persistent nephrotic syndrome; and five patients are free from proteinaria, of whom one has developed hypertension. A good correlation was observed between the response of the rheumatic disease to chlorambucil treatment and the clinical course of renal symptoms. Fourteen renal biopsies were performed in these eight patients. In all, amyloid deposits were of the AA type, which persisted on repeat biopsies. In addition, 15%–60% of glomeruli had become globally sclerotic by the second or third biopsies. At the ultrastructural level, modifications in the structure of amyloid deposits and reparative changes of the glomeruli, characterized by partial restoration of glomerular architecture, were observed in three patients with a favourable clinical course.
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  • 36
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    Pediatric nephrology 4 (1990), S. 533-541 
    ISSN: 1432-198X
    Keywords: Henoch-Schönlein purpura ; Pathogenesis ; Genetics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Laboratory studies of the pathophysiology of Henoch-Schönlein purpura (HSP) have become more numerous in recent years with the recognition of the disease's links with the mucosal immune system in general and IgA nephropathy in particular. There are weak genetic associations with C4 null phenotypes and with HLA B35 and DR4. Studies of plasma proteins in HSP patients show an increased IgA concentration, activation of the alternative pathway of complement and consumption of factor XIII. High molecular weight (polymeric) IgA has been detected in affected individuals, which some investigators have called “immune complexes”. Many patients synthesise an IgA rheumatoid factor in the acute phase, but other autoantibodies are largely absent. In vitro studies of lymphocytes from HSP patients have demonstrated an increased number of IgA-bearing and secreting B-cells, with altered T-cell regulation of antibody synthesis. While these observations point to immune dysregulation — primarily of IgA production — as a consistent feature of acute HSP, there is as yet insufficient information available to allow a consistent theory of pathogenesis to be formulated.
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  • 37
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    Sexual plant reproduction 3 (1990), S. 31-34 
    ISSN: 1432-2145
    Keywords: Mucoraceae ; Zygomycetes ; Homothallic ; Genetics ; Nutritional complementation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Auxotrophic strains of Mucor genevensis and Zygorhynchus exponens were crossed and the resulting zygospores germinated. The presence of a true sexual cycle in both species was demonstrated by the recovery of recombinant genotypes. Expected Mendelian ratios were not realized, however. The presence of selfed zygospores among those isolated makes this observation understandable. It was possible to demonstrate nutritional complementation when young mating mycelium was transferred to minimal medium and forced heterokaryons were recovered.
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  • 38
    ISSN: 1432-1076
    Keywords: Electron microscopy ; Liver ; MCAD deficiency ; Reye syndrome
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Inborn errors involving the oxidative metabolism of fatty acids may present clinically with a Reye syndrome-like picture. This case report of a patient with medium-chain acyl CoA dehydrogenase (MCAD) deficiency illustrates that electron microscopy may help to differentiate this disorder from Reye syndrome even if a liver biopsy is performed in a patient who recovered from an acute metabolic decompensation. Together with this case, a review of the few reports in the literature of pathological findings in MCAD deficiency is given. Changes uncharacteristic for Reye syndrome are a largedroplet steatosis and the presence of distinctive mitochondrial abnormalities on electron microscopy. The detection of an electron dense mitochondrial matrix and a widened space of inner mitochondrial membranes rules out Reye syndrome and is suggestive of a disorder of mitochondrial fatty acid oxidation.
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  • 39
    ISSN: 1432-1076
    Keywords: Osteogenesis imperfecta ; Collagen type I ; Radiology, classification ; Genetics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Congenital osteogenesis imperfecta (OI) was diagnosed by ultrasound in a 31-week-old fetus, and the diagnosis confirmed after delivery by caesarean section at week 36. The baby survived the neonatal period, but failed to thrive, had recurrent respiratory infections and ultimately died at 8 months. Cultured fibroblasts synthesized both normal type I collagen and unstable type I collagen harbouring a structural defect in the α1(I) cyanogen bromide-derived peptide number 8 (CB8) region of the molecule, indicating a heterozygous dominant mutation. A+ birth, the radiological picture was that of the “thin bone”-type of congenital OI (OI type IIB/III in the Sillence classification); at the age of 12 weeks ribs and long bones had undergone a marked expansion giving a very different picture, that of the “thick bone”-type congenital OI (OI type IIA). The mechanism responsible for this change in bone structure is not known, but fractures and callus formation are unlikely to be the only factors. Caution is needed in the interpretation of radiographs of newborns with OI for prognostic or genetic purposes.
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  • 40
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    Journal of comparative physiology 166 (1990), S. 545-552 
    ISSN: 1432-1351
    Keywords: Honeybees ; Learning ; Classical conditioning ; Selection ; Genetics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Four strains of the honeybee (Apis mellifera capensis), which were selected for high (N=2) or low (N=2) performance levels in classic conditioning of olfactory and mechanosensory stimuli, were examined in two instrumental visual learning tasks. Bees were trained to coloured cardboards either at the hive entrance or at the feeding station. Positive correlations were detected between olfactory/mechanosensory conditioning and visual learning. Good and poor learners from strains selected for olfactory conditioning differed significantly in their visual learning values. These strain differences reflect genetic differences in a common learning system rather than task specific differences in sensory, motor or motivational components. Parameters that were influenced by activity of the colony (duration of stay at the feeding place, time between visits) also differed among selected strains. These effects were not due to selection. Instead, they reflect a specific genetic background produced in each strain independently of selection. The results indicate that associative learning has a genetic basis which is independent of the sensory stimuli associated with reward, the learning procedure (classical conditioning or instrumental learning) or the motor patterns used to execute the learned behavior (proboscis extension, control for flight behavior, open field orientation).
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  • 41
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    Journal of neurology 237 (1990), S. 39-41 
    ISSN: 1432-1459
    Keywords: Progressive subcortical vascular encephalopathy ; Electron microscopy ; Nerve fibres ; Corpus callosum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The nerve fibres of the corpus callosum were studied by electron microscopy in five elderly patients with progressive subcortical vascular encephalopathy (PSVE) and compared with those in six age-matched controls. The number of nerve fibres per unit area of the corpus callosum was decreased in PSVE by 18–26%. The loss of nerve fibres in the corpus callosum can play a role in inducing the cognitive deficit of PSVE, on the basis of the loss of nerve fibres in the cerebral hemispheres.
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  • 42
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    Journal of neurology 237 (1990), S. 88-93 
    ISSN: 1432-1459
    Keywords: Amyloid-rich primitive plaque ; Diffuse plaque ; Amyloid ; Periodic-acid methenamine silver method ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In Alzheimer-type dementia brains, numerous “amyloid-rich primitive plaques (PPs)” were observed with β-protein immunostaining and periodicacid methenamine (PAM) staining. These amyloid-rich primitive plaques were accompanied by various degrees of small argyrophilic rod-like, granular or filamentous structures. Routine and modified-PAM electron microscopy revealed many bundles and flecks consisting of amyloid fibrils scattered widely throughout the plaques. Degenerate neurites, astrocytic processes and bundles of glial fibres also participated in the formation of the plaques. The similarities and differences between these amyloid-rich primitive plaques and diffuse plaques are described.
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  • 43
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    Journal of neurology 237 (1990), S. 382-384 
    ISSN: 1432-1459
    Keywords: Intracerebral sarcoma ; Meningeal sarcoma ; Light microscopy ; Immunohistochemistry ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A case of a primary intracerebral sarcoma is described in a 5-year-old girl. Histology and immunohistochemistry excluded the diagnosis of a leiomyosarcoma, a malignant haemangiopericytoma or a fibrosarcoma; electron-microscopical findings indicated that the origin of the sarcoma was in the pia mater.
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  • 44
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    Journal of cancer research and clinical oncology 116 (1990), S. 517-524 
    ISSN: 1432-1335
    Keywords: Tumor vascularization ; Tumor blood flow ; Xenograft ; Nude mouse ; Vascular corrosion cast ; Ultrastructure ; Electron microscopy ; Heterogeneity ; Vessel morphology ; Morphometry ; Necrosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary This study was designed to examine the vascular system of human xenotransplanted tumors on nude mice with different complementary morphometrical and morphological methods. The vascular system shows a chaotic arrangement. There is an extreme heterogeneity in the vascular distribution and density. Large avascular regions could be identified in several non-necrotic tumors. There was no clear difference in the vascular density between the center and the periphery of the tumors, nor was there any zonal correlation for the distribution of the necrosis. With three-dimensional corrosion casts it could be demonstrated that clusters of vessels were directly beneath areas almost free of vessels. In the center, vessels often form a sinusoidal system with numerous blind ends without clearly discernible endothelial cells. Numerous irregular tumor-cell-lined sinusoids are visible next to endothelial-lined vessels with transmission electron microscopy. With scanning electron microscopy it could be demonstrated that large-calibre endotheliazed vessels were found in the direct vicinity or in the center of non-viable zones. Even large-calibre vessels have a capillary wall structure. Sometimes, a basement membrane cannot be observed at all or only incompletely. There are numerous indications of vascular discontinuities and leaks with a widespread intercellular occurrence of blood cells.
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  • 45
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    European archives of psychiatry and clinical neuroscience 239 (1990), S. 290-292 
    ISSN: 1433-8491
    Keywords: Schizophrenia ; Linkage ; Genetics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary We analysed six multiplex pedigrees of schizophrenia for linkage to two DNA probes mapping to the chromosome 5q11–q13 region where linkage to a gene for schizophrenia was recently reported. Analyses were conducted using three penetrance models and considering the affected state to be schizophrenia, the schizophrenia spectrum, and all psychiatric diagnoses. All analyses gave consistently negative lod scores. Although the region was not formally excluded, no evidence for linkage was found.
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  • 46
    ISSN: 1432-1459
    Keywords: Spinocerebellar degeneration ; Friedreich's disease ; Diagnostic criteria ; Genetics ; Natural history
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The clinical and genetic features of 80 patients with Friedreich's disease from 64 families are described. Diagnostic criteria were: no evidence of dominant inheritance, onset by the age of 20 years, progressive unremitting ataxia of limbs and gait, and absence of knee and ankle jerks. Furthermore, at least one of the following accessory signs was present: dysarthria, extensor plantar response and echocardiographic evidence of hypertrophic cardiomyopathy. Two peaks of onset age were evident at 6–9 and 12–15 years. Analysis of intrafamily variation of onset age and absence of clustering of cardiomyopathy and diabetes did not suggest genetic heterogeneity. Peripheral nerve impairment was an early finding and showed slight further progression, whereas involvement of the cerebellar and corticospinal pathways appeared later and mainly accounted for the progressive worsening of the disease.
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  • 47
    ISSN: 1432-1343
    Keywords: Analysis of variance ; Choropleth map ; Ecology ; Genetics ; Geography ; Permutation test ; Spatial autocorrelation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Description / Table of Contents: Résumé Cet article présente une solution au problème de l'analyse de variance, pour certains cas où la variable à analyser est spatialement autocorr élée alors que le critère de classification représente des sous-régions connexes du territoire à l'étude. On sait que les méthodes classiques d'analyse de variance ne sont pas applicables dans ce type de situation puisque la condition d'indépendance des échantillons n'est pas respectée; l'autocorrélation positive réduit la variabilité intragroupe, si bien que la quantité relative de variabilité intergroupe s'en trouve artificiellement augmentée. Cette situation correspond en réalité à une vaste catégorie de problèmes en génétique des populations, en écologie et dans d'autres branches de la biologie, ainsi qu'en épidémiologie, en géographie, en géologie, en science économique, en science politique et en sociologie. Ce nouveau test appartient à la famille des tests par permutation. Nous calculons la somme des dispersions intragroupes et testons contre une distribution de référence obtenue en permutant les régions géographiques un grand nombre de fois sur la carte. La véritable difficulté de ce test est d'ordre algorithmique, puisqu'il n'est pas facile de permuter des régions sur une carte, de façon à ce que chaque groupe demeure connexe, et que la carte permutée occupe le même espace total que la carte d'origine. Cet article présente la théorie, les algorithmes, ainsi que des résultats obtenus par cette méthode. Un programme écrit en PASCAL est disponible.
    Notes: Abstract The classical method for analysis of variance of data divided in geographic regions is impaired if the data are spatially autocorrelated within regions, because the condition of independence of the observations is not met. Positive autocorrelation reduces within-group variability, thus artificially increasing the relative amount of among-group variance. Negative autocorrelation may produce the opposite effect. This difficulty can be viewed as a loss of an unknown number of degrees of freedom. Such problems can be found in population genetics, in ecology and in other branches of biology, as well as in economics, epidemiology, geography, geology, marketing, political science, and sociology. A computer-intensive method has been developed to overcome this problem in certain cases. It is based on the computation of pooled within-group sums of squares for sampled permutations of internally connected areas on a map. The paper presents the theory, the algorithms, and results obtained using this method. A computer program, written in PASCAL, is available.
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  • 48
    ISSN: 1432-2242
    Keywords: Genetics ; Symbiosis ; Nitrogen fixation ; Coevolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary To determine the relationship between nodulation restriction by the Rj4 allele of soybean, rhizobitoxine-induced chlorosis, and taxonomic grouping of bradyrhizobia, 119 bradyrhizobial isolates were tested in Leonard jar culture for nodulation response and chlorosis induction. In addition to strain USDA 61, the strain originally reported as defining the Rj4 response, eight other isolates (i.e., USDA 62, 83, 94, 238, 252, 259, 260, and 340) were discovered to elicit the nodulation interdiction of the Rj4 allele. Only 16% of all the bradyrhizobial strains tested induced chlorosis, but seven of the nine strains (78%) interdicted by the Rj4 allele were chlorosis-inducing strains. Furthermore, in tests for antibiotic resistance profile, eight of the nine interdicted strains (89%) were classed in DNA homology group II. This evidence suggests that the Rj4 allele has a positive value to the host plant in shielding it from nodulation by certain chlorosis-inducing bradyrhizobia of a DNA homology group with impaired efficiency of nitrogen fixation with soybean.
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  • 49
    ISSN: 1432-2242
    Keywords: Beta vulgaris ; Sugar beet ; Isozymes ; Genetics ; Linkage ; Pollen fertility restorer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The NADP-specific malate dehydrogenase isozymes were controlled by multiple gene systems. Three genes coding for dimeric enzymes segregated in a dependent fashion (NADP-Mdh 1, NADP-Mdh 2, NADP-Mdh 3). A fourth gene (NADP-Mdh 4), also coded for dimers, but was not polymorphic in B. vulgaris. A fifth gene (NADP-Me 1) coded for enzymes active as monomers. Two genes were found to control the main zone of NAD-specific malate dehydrogenase: one coded for dimers (Mdh 1), while a second (Mdh 2) was not polymorphic in the assessions studied. 6-P-Gluconate dehydrogenase was not polymorphic in B. vulgaris; the two types detected on SGE1 electrophoresis were due to developmental expression of the different systems. No genetical segregations could be detected in progeny of crosses of the distinct phenotypes. A shikimate dehydrogenase gene (Skdh 1) that coded for monomers was identified. The diaphorase system was rather complex, but one gene (Dia 1) coding for monomeric enzymes could be identified. Aconitase was found to be controlled by two independent genes (Aco 1, Aco 2), both polymorphic and coding for proteins active as monomers. Tight linkage was found between the genes NADP-Mdh 1, NADP-Mdh 2 and NADP-Mdh 3. Linkage was also found between a pollen fertility restorer (Z) and the Mdh 1 gene. The identification of linkage with Aco 1 needs further investigation. R segregated independently from Mdh 1, Aco 1 and Dia 1. Independent segregations were scored for isozyme genes Pgm 2, Icd 1, Ak 1, Gpi 1, Aco 1 and Dia 1.
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  • 50
    ISSN: 1432-0878
    Keywords: Testis ; Mast cells ; Estrogen ; Cytochemistry ; Electron microscopy ; Rat, Wistar
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The accumulation of mast cells in the testicular interstitium of neonatally estrogen-treated rats was studied from 15 to 90 days of age. The maturation of these cells was assessed by ultrastructural analysis and their histochemical properties were examined with the sequential alcian blue-safranin staining method. The first identifiable mast cells appeared in the testis at 17–20 days of age, as immature cells with proliferative capacity. The density of mast cells increased up to 45 days of age, showing a slight decrease from 45 to 90 days of age. Before 45 days of age, most mast cells showed alcian blue-stained granules, whereas at 45 days of age, most cells presented a mixture of alcian blue and safranin-stained granules. From this age onward, most cells were stained with safranin. These maturational changes were well-correlated with their ultrastructural features. Mast cells presented few and heterogeneous immature granules up to 45 days of age, and many uniform electron-dense granules at 90 days of age. These results indicate that the testicular interstitium of neonatally estrogen-treated rats provides an adventageous environment for the recruitment, proliferation and maturation of connective tissue mast cells.
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  • 51
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    Cell & tissue research 260 (1990), S. 41-48 
    ISSN: 1432-0878
    Keywords: Keratin filament ; Circumvallate papilla ; Taste bud ; Immunocytochemistry ; Electron microscopy ; Mouse (dd)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Keratin filaments of epithelial- and taste-bud cells in the circumvallate papillae of adult and developing mice were studied by immunocytochemistry using monoclonal antikeratin antibodies (PKK2 and PKK3) and by conventional electron microscopy. Elongated cells (type-I,-II, and-III cells) of the taste buds were stained by PKK3 antibody, which reacts with 45-kdalton keratin, whereas basal cells of the taste buds and surrounding epithelial cells showed negative staining with PKK3. Such PKK3-reactive cells occurred at 0 day after birth, when taste-buds first appeared in the dorsal surface epithelium of the papillae. Thus 45-kdalton keratin seems to be an excellent immunocytochemical marker for identifying taste-bud cells. Epithelial cells in all layers of the trench wall and basal layer cells of the dorsal surface contained densely aggregated bundles of keratin filaments that reacted with PKK2 antibody, but not with PKK3. In contrast, taste-bud cells and spinous and granular layer cells of the dorsal surface possessed loose aggregated bundles of filaments that reacted with PKK3, but not with PKK2. These results suggest that the aggregation and distribution pattern of keratin filaments may reflect differences in the keratin subtypes that comprise these filaments.
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  • 52
    ISSN: 1432-0878
    Keywords: Vitamin A-deficiency ; Paneth cells ; Crystalloid lysozyme ; Tubular structures ; Intestinal local immunity ; Electron microscopy ; Rat (Sprague-Dawley)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The effect of vitamin A-deficiency on jejunal Paneth cells in rats was investigated. Crystalloid particles were observed in secretion granules of Paneth cells from 6 out of 8 rats with vitamin A-deficiency. The particles were similar to those found in Paneth cells under other experimental conditions. Using an immuno-electron-microscopic technique we demonstrated a clear lysozyme immunoreactivity of these particles. In 2 vitamin A-deficient rats tubular structures have been detected in addition to the crystalloid particles. Crystalloid particles or tubular structures were not detectable in a control group of 8 vitamin A-supplemented rats. The morphological alterations of Paneth cells may be correlated to an impaired local immunity of the intestine during vitamin A-deficiency.
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  • 53
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    Cell & tissue research 260 (1990), S. 409-414 
    ISSN: 1432-0878
    Keywords: Kidney ; Peroxisomes ; Marginal plates ; Electron microscopy ; Freeze-etching ; DAB-cytochemistry ; Bovine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The ultrastructure of peroxisomes in the proximal nephron tubules of bovine kidney cortex was studied using ultrathin-sectioning, diaminobenzidine cytochemistry for the visualization of catalase, and by freeze-fracture. Peroxisomes in this nephron segment are up to 1.5 μm in diameter and exhibit a peculiar angular shape, which is probably related to the occurrence of multiple straight plate-like inclusions (marginal plates) in the matrix of peroxisomes; they lie directly underneath the peroxisomal membranes. The peroxisomal membrane in such regions follows the outline of the marginal plate. The peculiar shape of peroxisomes allows their unequivocal identification in freeze-fracture preparations. Peroxisomal membranes are recognized by their flat, often rectangular appearance. Intramembrane particles are much more numerous on P-fracture faces than on E-fracture faces. A crystalline lattice-structure with a periodicity of approximately 10 nm can be observed on the flat rectangular areas of E-fracture faces. This lattice structure is intensified after prolonged freeze-etching. Intramembranous particles seem to be superimposed over this pattern. The crystalline pattern on the E-fracture faces of peroxisomal membranes is probably not a membrane structure but it reveals the structure of the membrane-associated marginal plates. A cast of the marginal-plate surface may be generated by a collapse of the peroxisomal membrane half onto the immediately underlying matrix inclusion.
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  • 54
    ISSN: 1432-0878
    Keywords: Neuropeptide Y ; Arginine vasopressin ; Co-storage ; Immunohistochemistry ; Electron microscopy ; Paraventricular nucleus ; Rat (Sprague-Dawley)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Certain populations of arginine vasopressin (AVP) neurons in the magnocellular paraventricular nucleus became immunoreactive for neuropeptide Y (NPY) when rats were treated with colchicine or monosodium glutamate (MSG). The co-storage of these peptides was examined by empooying a post-embedding electron-microscopic immunohistochemistry technique using goldlabeled antibodies to the two peptides. In colchicinetreated rats, the neuronal perikarya contained numerous secretory granules showing co-storage of the two peptides. The cells of the MSG-treated rats were characterized by having well-developed Golgi bodies with the granular structures also co-storing the two peptides, although the secretory granules in the perikarya were rather fewer than in the colchicine-treated rats. It is concluded that the destruction of the arcuate nucleus by MSG-treatment may potentiate the synthesis of NPY in AVP neurons, the synthesis of which is latent in intact animals.
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  • 55
    ISSN: 1615-6102
    Keywords: Anther embryogenesis ; Brassica napus ; Electron microscopy ; Histochemistry ; Microspore embryogenesis ; Pollen division
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Ultrastructural and cytochemical features of embryo development during anther and free microspore culture inBrassica napus have been followed from the late uninucleate microspore stage through the first embryonic division. On transfer to culture, the microspore cytoplasm possesses a large vacuole, often containing electron opaque aggregates, and a peripheral nucleus. Mitochondria, endoplasmic reticulum and starch-free plastids are distributed throughout the cytoplasm. The conditions of culture induce a number of major changes in the cytoplasmic organisation of the microspore. First, the central vacuole becomes fragmented allowing the nucleus to assume a central position within the cell. Secondly, starch synthesis commences in the plastids which, in turn, are seen to occupy a domain investing the nucleus. Thirdly, the cell develops a thick fibrillar wall, situated immediately adjacent to the intine of the immature pollen wall. Finally, the microspores develop large cytoplasmic aggregates of globular material. The nature of this substance remains unknown, but it remains present until the young embryos have reached the 30 cell stage. The first division of cultured microspores destined to become embryos is generally symmetrical, in contrast to the asymmetric division seen in normal development in vivo. Consideration is given to the differences observed between embryos developing from anthers and free microspores in culture.
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  • 56
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    Protoplasma 153 (1990), S. 149-156 
    ISSN: 1615-6102
    Keywords: Acid phosphatase ; Electron microscopy ; Mammary glands ; Subcellular fractions ; Substrate specificity ; Sulfhydryl agents ; Tartrate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Localization of acid phosphatase in mammary glands of lactating rats was studied by both biochemical and cytochemical methods. Cytochemically, acid phosphatase activity was detected by using lead citrate as the capture agent for the inorganic phosphate released from p-nitrophenyl phosphate. The activity was predominantly localized in the lumina of the endomembrane system and in the milk that had been secreted into the alveolar lumen. Biochemically, acid phosphatase was present in all the subcellular fractions with higher activities in the membrane-associated fractions. The localization of tartrate-resistant acid phosphatases within the endomembrane system of fully lactating rat mammary tissue suggests a possible role for these enzymes in milk secretory processes.
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  • 57
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    Protoplasma 154 (1990), S. 132-143 
    ISSN: 1615-6102
    Keywords: Vigna sinensis ; Immunofluorescence ; Electron microscopy ; Colchicine ; Tubulin reticulum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The primary leaf, epicotyl, and root cells ofVigna sinensis seedlings grown continuously in a 0.08% colchicine solution, become microtubule-free and polyploid. In meristematic root cells a tubulin transformation is detected 1–3 h after the treatment had begun. Tubulin strands are organized at the positions of the pre-existing microtubules. Frequently, the strands converge on or are organized in the cortical cytoplasmic zone where in normal cells the preprophase microtubule band (PMB) is assembled. In meristematic root cells subjected to a 6–12 h colchicine treatment, the tubulin strands become perinuclear, entering the cortical cytoplasm at regions close to the nucleus. One day after the onset of the treatment, tubulin generally forms a continuous reticulum of interconnected strands in all the organs examined. In most cells this reticulum surrounds the nucleus partly or totally or lies close to it, exhibiting variable configurations in different cells. After prolonged treatments, the organization of the tubulin reticulum changes further. Now this consists of crystal-like structures interconnected by thin strands. On thin sections of fixed tissue the tubulin strands consist of paracrystalline material. The distribution of this material in the affected cells coincides with that of tubulin reticulum visualized by immunofluorescence. In transverse planes each strand exhibits circular subunits arranged close to one another in a hexagonal pattern but in longitudinal ones variable images were observed. The paracrystalline material persists in root cells subjected to an 8-day continuous colchicine treatment. The immunolabeled strands seem to be composed of tubulin-colchicine complexes and not pure tubulin.
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  • 58
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    Biology and philosophy 5 (1990), S. 349-371 
    ISSN: 1572-8404
    Keywords: Genetics ; gene structure ; hereditary unit
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Philosophy
    Notes: Abstract Definitions of the term ‘gene’ typically superimpose molecular genetics onto Mendelism. What emerges are persistent attempts to regard the gene as a ‘unit’ of structure and/or function, language that creates multiple meanings for the term and fails to acknowledge the diversity of gene architecture. I argue that coherence at the molecular level requires abandonment of the classical unit concept and recognition that a gene is constructed from an assemblage of domains. Hence, a domain set (1) conforms more closely to empirical evidence for genetic organization of DNA regions capable of transcription and (2) has ontological properties lacking in the traditional unit definition.
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  • 59
    ISSN: 1573-5133
    Keywords: Demography ; Genetics ; Geographic variation ; Stochasticity ; Fluctuating environments ; Allele frequencies ; River ecology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Synopsis The purpose of this study was to determine the effects of unpredictable environmental fluctuations on the demographic and genetic structure of Fundulus zebrinus populations. Collections of F. zebrinus were taken from three rivers in the Arkansas River basin: the Arkansas, Chikaskia, and Ninnescah. Fish were sampled from three sites on each river on nine collection dates throughout 1984 and 1985. Totals of 2100 fish and 6000 fish were included in electrophoretic and demographic analyses, respectively. The results of the study indicate that within a limited geographic region (i.e. within rivers) spatial differences and temporal changes in both demographic and genetic population characteristics occur frequently and are primarily stochastic. However, on a larger spatial scale (i.e. across rivers), general trends emerge for demographic and especially for genetic population characteristics. These results illustrate the importance of sampling scale for conclusions of life-history evolution in fluctuating environments. In addition, it was found that regulation of Fundulus zebrinus populations includes an important density-independent component. Stochastic demographic differences across space and changes through time and spatially and temporally heterogeneous allele frequencies, are both indicative of density-independent regulation. Variation in population parameters, both demographic and genetic, was observed between populations sampled from each river. These population differences were attributed to differences between the rivers themselves.
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  • 60
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    Journal of chemical ecology 16 (1990), S. 2935-2946 
    ISSN: 1573-1561
    Keywords: Genetics ; sex pheromone ; Lepidoptera ; Noctuidae ; Trichoplusia ni ; cabbage looper moth ; reproductive isolation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The genetic basis of interpopulational differences in the pheromone blend emitted by the cabbage looper moth,Trichoplusia ni (Hübner), was examined by crossing individuals from a field-derived population (P1) with individuals from a long-maintained laboratory colony (P2). These colonies differed in the emission rate and relative proportions of four of the five known minor pheromone components, but not in the emission rate of the major component, (Z)-7-dodecenyl acetate (Z7-12∶Ac). These differences in pheromone blend were quantitatively small but biologically significant, because in the field, males responded preferentially to traps baited with a pheromone blend that is similar to that emitted by P1 females relative to a blend similar to that emitted by P2 females. In initial crosses, variation in the quantity and quality of pheromone blends among families of P1, P2, and F1 hybrid females was examined. In F1 females the relative proportions (quantity relative to the major component) of (Z)-5-dodecenyl acetate (Z5-12∶Ac) and (Z)-7-tetradecenyl acetate (Z7-14∶Ac) were intermediate to parental lines. In a second more extensive set of crosses, analyses included P1, P2, F1, F2, and selected backcrosses. The relative proportion of Z5-12∶Ac, Z7-14∶Ac, and Z9-14∶Ac emitted by F1 females were intermediate to parental lines. The frequency distributions of relative proportions of these components emitted by females were not consistent with those expected under a single autosomal or sex-linked gene hypothesis, suggesting that more than one gene is involved in the quantitative differences in the pheromone blend.
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  • 61
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    European archives of oto-rhino-laryngology and head & neck 247 (1990), S. 189-193 
    ISSN: 1434-4726
    Keywords: Spiral ganglion ; Lipofuscin ; Ceroid ; Vitamin E deficiency ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The purpose of the present study was to clarify morphological differences in lipofuscin or so-called age pigments observed in the spiral ganglion cells of both young and adult rat groups and to characterize the size and structure of ceroid pigment granules generated in vitamin-E-deficient rats. The results showed different patterns of lipofuscin distribution in the two groups. The adult rat group had large aggregated lipoid, dark pigment granules of irregular shape in the cytoplasm. In contrast, the young group had small numbers of small, dense homogeneous granules, suggesting higher Schwann cell phagocytic activity. The ceroid pigments apparently included numerous vesicles and droplets of more variable density and size than the lipofuscin pigments appearing in the non-treated older animals. Both lipofuscin and ceroid pigments developing in such non-dividing cells are produced as a result of peroxidation reactions, so that the more they accumulate in the cytoplasm the more likely cell function deteriorates. The present study has shown that lipofuscin/ceroid granules are generated in the spiral ganglions under either endogenous (aging) or exogenous (vitamin E deficiency) conditions.
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  • 62
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    European archives of oto-rhino-laryngology and head & neck 247 (1990), S. 237-239 
    ISSN: 1434-4726
    Keywords: Hyaluronate ; Hyaluronidase-gold ; Nasal mucosa ; Goblet cells ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The ultrastructural distribution of hyaluronate in the nasal mucosa of Wistar rats, especially in secretory cells, was examined by hyaluronidase-gold labeling using electron microscopy. The main secretor of hyaluronate in the nasal mucosa was found to be the goblet cells and not the nasal glands.
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  • 63
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    European archives of oto-rhino-laryngology and head & neck 247 (1990), S. 240-243 
    ISSN: 1434-4726
    Keywords: Organ of Corti ; Limax flavus ; Feutin-gold ; Electron microscopy ; Sialic acid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The localization of sialic acid (N-acetyl- and N-glyconylneuraminic acid) in the organ of Corti of the mongolian gerbil was examined with electron microscopy by postembedding labeling using Limax flavus agglutinin and feutin-gold. Gold labeling was observed on the fibrous structure of the tectorial membrane, the basilar membrane and the spiral limbus. The labeling was also observed on the cuticular plate of the hair cells, the head and cone of the pillar cells and the head plate of Deiters' cells and other supporting cells. Collagen or cytoskeletal glycoproteins are suggested to be the source of the sialic acid in these structures.
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  • 64
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    European archives of oto-rhino-laryngology and head & neck 248 (1990), S. 57-62 
    ISSN: 1434-4726
    Keywords: Vestibular membrane ; Reissner's membrane ; Mesothelium ; Fixation ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The mesothelial cells of Reissner's membrane in guinea pigs were found to be connected by junctional complexes. No cell discontinuities or gaps were observed by scanning or transmission electron microscopy. These results are not in accordance with previous studies. They were achieved by in vivo vascular perfusion fixation, handling of cochleae in protective specimen carriers, thiocarbohydrazide treatment and continuous dehydration. Findings in the present study indicate that the interepithelial space between the epithelial and mesothelial cell layers constitutes a specific compartment which must be considered when examining solute transport over Reissner's membrane.
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  • 65
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    Stamford, Conn. [u.a.] : Wiley-Blackwell
    Polymer Engineering and Science 30 (1990), S. 22-29 
    ISSN: 0032-3888
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Polymer blends of nylon 66 and thermotropic polyester with long flexible spacers in the main chains were prepared by melt mixing. The samples were made as single filaments by passing the polymer blend through a small and round die of a capillary rheometer. Mechanical properties of blends showed that the modulus and strength of nylon 66 could be improved without reduction of extensibility. The morphology of fractured surfaces was observed by a scanning electron microscope (SEM). It showed that the microfibrillar structure of a thermotropic polyester was formed by extensional flow while the spherical and ellipsoidal particles in the nylon 66 (matrix polymer) were produced by shear flow. The polyester particles were occasionally covered with adhering matrix polymer because of good adhesion between these two polymers. They were highly elongated by tensile stress without loss of elongational characteristics of blends. This fact was explained by very good adhesion between the two phases.
    Additional Material: 8 Ill.
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  • 66
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    Polymer Engineering and Science 30 (1990), S. 83-89 
    ISSN: 0032-3888
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: The physical behavior of the diglycidyl ether of bisphenol A, cured with different concentrations of 2-ethyl-4-methylimidazole, was examined with dynamic mechanical and dielectric analyses, differential scanning calorimetry, and solvent extraction studies, ‘The network formation process was shown to depend strongly on the imidazole concentration’. At high imidazole concentrations, the gel point was characterized by a decrease in sol fraction, the crossover of the dynamic moduli and a rapid increase in viscosity. At low imidazole concentrations, the viscosity remained low until the sol fraction approached zero. For this system, the gel point, which occurred prior to the dynamic moduli crossover, was characterized by comparing the thermal properties of the network with the viscosity and dielectric behavior of the resin system during cure.
    Additional Material: 12 Ill.
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    Polymer Engineering and Science 30 (1990), S. 108-117 
    ISSN: 0032-3888
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: We examine several practically important problems, related to the mechanical behavior of dual coated optical fibers: low temperature microbending, evaluation of spring constant due to coating layers, strength of the end portions of fibers clamped in terminal fixtures, and prediction of stresses caused by the misalignment of the openings in the frame and in the terminal fixture. The developed formulas are simple, easy-to-use, and clearly indicate the role of the major factors affecting the mechanical behavior of the fibers. The obtained results can be of help in physical design of dual coated fibers and optical interconnections.
    Additional Material: 7 Ill.
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  • 68
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    Polymer Engineering and Science 30 (1990), S. 129-133 
    ISSN: 0032-3888
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: The “Efficiency of Energy Absorption” (or “Efficiency”) and “Ideality” parameters were evaluated for several plastic foams and were found to be very useful in choosing an appropriate cushion for the protection of a specific product. The maxima in these parameters were found to be in the same range of stresses, when derived from dynamic measurements or from predicted stress-strain curves based on previously proposed “Reference” and “Modified Boltzman Superposition” Models. For the rate independent foams the maxima in these parameters do not change with strain rate and can therefore be derived from slow, constant rate (“static”) experiments. For rate dependent foams however, the maxima from “static” measurements were found to be in a lower range of stresses than those derived from dynamic ones. As a result, slow rate compressive measurements do not predict well the behavior of the foams during impact and the use of the “Reference” and “Modified Boltzman Superposition” Models is required for good predictions. It was found that the suffer PS and PE foams attain maximum Efficiency and Ideality at higher stresses than the more flexible PUR foams.
    Additional Material: 11 Ill.
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    Polymer Engineering and Science 30 (1990), S. 96-107 
    ISSN: 0032-3888
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Sudden changes of compressive strain-rate on glassy poly(methyl methacrylate) lead to the conclusion that the post-yield state under a constant stress is a state of steady flow in the polymer. Non-linearity between the stress divided by temperature and the logarithm of strain rate for this steady plastic flow can successfully be analyzed using the Eyring equation with structural factors variable depending on stress and temperature. This analysis gives a unique functional relationship between the activation entropy and the activation enthalpy, which agrees fairly well with that for the melt derived from the WLF equation. This agreement provides a direct evidence verifying structural change of the glass into liquid-like structure and enables us to recognize the conformational arrangement as an essential structural parameter controlling molecular mobility. Another experimental relation between the Eyring factors - the activation volume and the activation enthalpy - permits us to estimate the magnitude of an elementary volume for the molecular movement in the melt as a function of temperature. Moreover, the experimental finding of the steady flow in the glassy polymer motivated us to propose a transition mechanism from elastic deformation to plastic flow in which the internal viscosity or the activation volume is introduced as a single parameter representing the transient state of the structure.
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  • 70
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    Stamford, Conn. [u.a.] : Wiley-Blackwell
    Polymer Engineering and Science 30 (1990), S. 118-123 
    ISSN: 0032-3888
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: An apparatus was designed and assembled to study the solvent removal from solution-cast thin polymer films. The computer interfacing of a thermogravimetric analyzer, spectrophotometer, electronic flowmeters, and control valves for the apparatus enabled the preprogramming of the carrier gas velocity, carrier gas solvent content, and temperature profiles to simulate the environment experienced in large parallel flow industrial driers. The apparatus has also been designed and operated to enable the visual observation of the drying film with an optical microscope. Initial experimental studies conducted with the apparatus involved the effect of temperature on solvent removal. The results indicate that high dryer gas temperatures can apparently cause skinning of the film surface resulting in slower solvent removal rates. The skin formation can be suppressed by higher solvent concentration in the carrier gas. The visual observations revealed the formation of standing waves in the film surface during drying at high gas velocities (〉2OO cm/min). The wave formation at least partially overcomes the effect of skinning by increasing the surface area of the film, and may be the manifestation of flow instabilities involving circulation within the film.
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  • 71
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    Stamford, Conn. [u.a.] : Wiley-Blackwell
    Polymer Engineering and Science 30 (1990), S. 147-152 
    ISSN: 0032-3888
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Ultrasonically induced bubble formation for the production of thermoplastic foam was investigated experimentally and theoretically as a basic study. A general purpose polystyrene and blends of low density polyethylene and polyethylene wax were saturated with nitrogen gas under various pressures and the ultrasonic excitation was applied to the polymer system upon release of gas pressure. The ultrasonic nucleation of bubbles in the polymer matrix was modeled by utilizing the classical nucleation theory. The negative pressure generated by the ultrasonic excitation was considered as the environmental pressure at the moment of nucleation. The experimental results showed that the heterogeneous nucleation must be used for ultrasonic foaming of the viscous fluid and the homogeneous nucleation for the low viscosity fluid. The theoretical analysis also indicated that the ultrasonic nucleation can be applied to the production of thermoplastic foam if the ultrasonic excitation generates large enough negative pressure.
    Additional Material: 10 Ill.
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  • 72
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    Stamford, Conn. [u.a.] : Wiley-Blackwell
    Polymer Engineering and Science 30 (1990), S. 7-12 
    ISSN: 0032-3888
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Extrusion measurements have been carried out on blends of nylon 6 and a liquid-crystalline copolyesteramide (LCP). The flow curves at low temperature show a behavior similar to that of pure LCP with a rapid rise of the viscosity at low shear rates. At high shear rates the viscosity is lower than that for each of the two components. This minimum has been attributed to the lack of interactions between the two phases and to the formation of fibrils of the LCP phase. The SEM analysis shows, indeed, that fibrils of the LCP phase are produced in the convergent flow at the inlet of the capillary at high shear rates. These fibrils are lost during the flow in the long capillary.
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  • 73
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    Polymer Engineering and Science 30 (1990), S. 124-128 
    ISSN: 0032-3888
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Rheological studies of an experimental liquid crystalline (LC) copolyester were carried out using a capillary rheometer and a cone and plate rheometer. Rheological characteristics of the polymer in the nematic state were observed. The nematic melt was found to be pseudoplastic and the degree of pseudoplasticity varied with shear rate. Melt viscosity was found to decrease with shear rate. Negative die swelling was observed at the exit of the capillary rheometer at temperatures marginally above the solid-nematic transition temperature of the polymer and was also found to be a function of shear rate. The dynamic mechanical properties of the polymer were studied as a function of temperature. The activation energies of flow and of dynamic mechanical deformation were calculated.
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  • 74
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    Stamford, Conn. [u.a.] : Wiley-Blackwell
    Polymer Engineering and Science 30 (1990), S. 137-141 
    ISSN: 0032-3888
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Ion beam mixing was used to improve the adhesion between deposited Cu film (400 Å) and polyimide (PI) substrate. Ar+ ion with the energy levels between 180 and 200 keV, and the dose between 1014 to 4 × 1016 ions/cm2 were used. The surface analyses were carried out by Rutherford Backscattering Spectroscopy (SEM). RBS analysis, using 2 MeV He+ ions, showed mixing of Cu and FI and the mixing depended on the Ar+ energy and dose. The X-ray study showed a very broad halo for deposited Cu film but the (111) peak appeared after the Ar+ implantation and the peak increased with Ar+ ion dose. Optical micrographs showed that Cu film formed circular bubbles after many thermal cycles when adhesion was poor and fracture cracks when adhesion was good.
    Additional Material: 7 Ill.
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  • 75
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    Polymer Engineering and Science 30 (1990), S. 153-161 
    ISSN: 0032-3888
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: The general domain in which this work resides is that of mixing in creeping flows. Mixing, in this context, refers to the stretch of an interfacial line, or area in a strain field. The advancement of mixing technology is applied to the design of continuous mixers used in polymer processing. The geometric designs included single screw extruders, static motionless mixers, and co- and counter-rotating twin screw extruders. The co-rotating twin screw extruder was chosen to be studied in detail since it enjoys wide applications and, for which, little understanding of the contribution to mixing in the different screw geometries is known. In order to evaluate the rate of mixing for the non-uniform strain history flows, the method for measuring mixing had to be reexamined and broadened. An automated method has been developed which incorporates a digital camera and a computer to analyze the cross-sections of interest. Two measures of mixing - the correlation function and the distribution function - are developed to describe mixing in these regimes. These measures are applied successfully to the mixer geometries revealing subtle differences as to the nature of mixing in each.
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  • 76
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    Polymer Engineering and Science 30 (1990), S. 175-186 
    ISSN: 0032-3888
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: This paper describes the shear imposed interfacial segregation of release systems for the facilitated attenuation of polyurethane (PU) adhesion to metal coun-terfaces using a RI-RIM system. It is shown that the migration rate of the dispersed release additives due to a shear imposed stress in the resin fluid is much greater than that arising from Fickian diffusion, thereby removing a vital constraint from conventional practice. The novel rotary injection RIM system is presented to simulate the on-line injection and shear induced interfacial segregation in model PU/abherent systems. A wide range of recipes comprising single (liquids or solids) and multicomponent (liquid-liquid and solid-liquid) release materials were injected into the polymerizing resin mixture to provide cohesively weak and friable “particle” boundary layer assemblies at the PU/metal interface. An instrumented Blister Test was employed to evaluate the quality of the molded interfaces in terms of adhesion and the concentration distribution of the injected species in the final cured moldings was determined through high pressure liquid chromatography (HPLC). A comparison of the results on the shear modified and the compounded interfaces confirm an accentuated lateral migration of the additives to the interface resulting in an appreciable diminution in the adhesion of the system. Finally, transport models are suggested to account for the observed augmented transport.
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  • 77
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    Polymer Engineering and Science 30 (1990) 
    ISSN: 0032-3888
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
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  • 78
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    Polymer Engineering and Science 30 (1990), S. 197-201 
    ISSN: 0032-3888
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: It is the purpose of the present publication to show that most of the phenomenological viscoelastic theories developed for bulk polymers can be readily adapted to plastic foams in order to evaluate their mechanical properties. In addition it will be demonstrated how the mechanical properties of open cell plastic foams can be predicted from a few simple compression and relaxation experiments.
    Additional Material: 7 Ill.
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  • 79
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    Polymer Engineering and Science 30 (1990), S. 211-218 
    ISSN: 0032-3888
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: We have extended the essential work of fracture technique to allow for the determination of the plane-strain essential work of fracture. The new technique is to measure the specific work of fracture as a function of ligament length in deeply double edge notched samples. This type of data is then experimentally corrected to remove the plastic work of fracture and leave only the essential work of fracture as a function of ligament length. By extrapolating the essential work of fracture to zero-ligament length, we claim to be measuring the plane-strain essential work of fracture. This new technique was applied to two rubber toughened nylons and to a series of polyethylenes. The plane-strain essential work of fracture was found to be independent of thickness. Where comparison can be made to J-integral testing, the plane-strain essential work of fracture was similar to the critical J-integral, JIc.
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  • 80
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    Polymer Engineering and Science 30 (1990), S. 228-234 
    ISSN: 0032-3888
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: The objective of this work has been to study composite systems in which carbon fibers are dispersed in a liquid crystal polymer matrix. The fundamental point of interest here has been the interfacial response that fiber surfaces can potentially induce in self-ordering polymers. The matrix material used was a thermotropic liquid crystal polyester synthesized in our laboratory from the monomers p-acetoxybenzoic acid, diacetoxyhydroquinone, and pimelic acid. The aromatic-aliphatic polymer was characterized by NMR as a chemically disordered polymer of the three structural units which exhibits a nematic phase at temperatures above 150°C. Breadline proton NMR above the solid to liquid crystal transition was used to measure the rate of magnetic alignment of molecules in the matrix and polarized optical microscopy was used to analyze interfacial zones in composite samples. Fiber surfaces were found to influence the orientation and orientational dynamics of a liquid crystal polymer matrix. This was revealed by enhanced rates of magnetic orientation in the polymer melt when carbon fibers are dispersed in the medium. Fiber surfaces were also found to stabilize nematic ordering of the polymer as the melt was heated towards complete isotropization. The phenomena discovered here may originate in the development of zones around fibers with a common molecular orientation anchored by the carbon surface.
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  • 81
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    Polymer Engineering and Science 30 (1990), S. 241-248 
    ISSN: 0032-3888
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: A series of impact tests are described in which the plane strain fracture toughness, Kc1, of five different polymers is measured using a three point bend specimen at striker speeds up to 5m/s. At low speeds Kc1 is determined using the maximum load and a static analysis, but at speeds greater than 1 m/s the dynamic effects render the load signal unusable. For the higher speeds the fracture is timed using contact and crack propagation gages and the analysis is performed using the striker displacement at fracture. A dynamic analysis is used to convert this measurement to the true specimen displacement and Kc1 is determined from this. The apparent downward trends in the Kc1 results obtained, especially at speeds above 3m/s, are discussed.
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  • 82
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    Stamford, Conn. [u.a.] : Wiley-Blackwell
    Polymer Engineering and Science 30 (1990) 
    ISSN: 0032-3888
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
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  • 83
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    Polymer Engineering and Science 30 (1990), S. 385-393 
    ISSN: 0032-3888
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: We have developed a new method to measure the true temperature of the polymer melt and its three-dimensional distribution of a shot stored in the reservoir during injection. By means of this method, it is demonstrated that the temperature distribution of the polymer melt stored in the reservoir is strongly dependent on the setup molding conditions, the polymer investigated, and the geometry of the screw used. Further, it is noted qualitatively that there are two major effects of screw geometry on the temperature distribution of polymer melts. One, which governs strongly the temperature in the high temperature zone, is shear heat generated within the polymer itself in the metering zone of the screw during the transporting process. The second is the ability to preheat and plasticate the polymer in the compression zone, which is indispensable for uniform plastication.
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  • 84
    ISSN: 0032-3888
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: The breaking strength, strain at break, and work to rupture of perfect fibers prepared with polymers of finite molecular weight are calculated by treating the perfect fiber as a stressed crystal undergoing a crystal-melt phase transition. In this view, a tensile load destabilizes the crystal and depresses its melting point. When the load is sufficient to lower the melting temperature to the ambient condition the fiber melts - i.e., fails. The theoretical equations (extremely simple) are applied to several common polymer fibers. The maximum tensile strength of polyethylene, for example, is calculated to be 7 to 9 GPa, in good agreement with current experimental results.
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  • 85
    ISSN: 0032-3888
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Vinyl polymers containing pendant acetal groups were synthesized using (2,2-dimethyl-l,3-dioxolan-4-yl)methyl acrylate (DMA) and (2,2-dimethyl-l,3-dioxo-lan-4-yl)methyl methacrylate (DMM), and were evaluated as negative electron beam (EB) resists. It was found that the EB sensitivity of polymers containing acetal groups in the side chain was higher than that of polymers containing acetal groups in the main chain. A high sensitivity of 3.6 × 10-8 C/cm2 was observed. Copolymers of DMA or DMM with styrene were also synthesized in order to improve the durability for dry etching process. It was found that the copolymers had an excellent dry etching durability and were adaptable to EB lithography.
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  • 86
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    Polymer Engineering and Science 30 (1990), S. 453-458 
    ISSN: 0032-3888
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: The thermal behavior of poly(etheretherketone)(PEEK) film heated in an open differential scanning calorimetry (DSC) pan at 20°C/min is distorted by relaxation of the strained film. PEEK film in a closed pan or quenched PEEK in open or closed pans shows a glass-transition temperature (Tg) around 144°C, cold crystallization (∼22 J/g) at 177°C, melt-temperature (Tm) peaking at 335-340°C, with an enthalpy of fusion of 32-34 J/g, and recrystallization on cooling at 285°C, with a crystallization exotherm of about 40 J/g. The enthalpy of fusion decreases with increasing heating rate from 2-100°C/min and approaches the enthalpy of cold crystallization. With increasing heating rate, further crystallization of PEEK during the DSC scan is suppressed. With increasing cooling rate, PEEK melt crystallizes at larger supercoolings to a lesser extent. Crystallization on cooling the melt was more complete than cold crystallization and annealing on heating.
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  • 87
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    Polymer Engineering and Science 30 (1990), S. 459-468 
    ISSN: 0032-3888
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: The electrical and dielectric properties of compression-molded segregated polyethylene-carbon black mixtures are described in the frequency range between 10 and 8000 kHz as a function of frequency, temperature, and carbon black loading. The segregated systems investigated exhibit insulator-conductor transitions in the range 0.25-0.65% (volume/volume) carbon black. The dielectric constant and the dissipation factor of the conductive samples are relatively very high in the frequency range studied. The dielectric constant increased sharply with the carbon black concentration, and then increased moderately beyond the insulator-conductor transition. The dissipation factor-concentration curves for different carbon blacks show maximum values in the vicinity of the critical concentration values. The dielectric properties of these systems are discussed in terms of interfacial Maxwell-Wagner polarization effects.
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  • 88
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    Polymer Engineering and Science 30 (1990), S. 476-479 
    ISSN: 0032-3888
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: The heat capacities of isotactic polypropylene and its composite with glass fiber have been measured at high pressure, up to 7 MPa, in the melt state by high pressure differential scanning calorimetry (HPDSC). The values also have been calculated from the data of specific volume (V) measured by dilatometry and thermal conductivity (κ) and thermal diffusivity (α) measured by a compensating hot wire method. The values of the heat capacity measured from HPDSC are consistent with those from the calculation method. The heat capacities of molten polypropylene and its composite increase linearly with temperature at a constant pressure and decrease with pressure at a constant temperature. It was found that the heat capacity of polypropylene is insensitive to molecular weight. The heat capacities of molten polypropylene composites are found to be predicted by an additive rule from the weight fractions of heat capacities of polypropylene and glass fiber.
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  • 89
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    Polymer Engineering and Science 30 (1990), S. 480-484 
    ISSN: 0032-3888
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: The mutual diffusion coefficients for styrene-butadiene rubber (SBR)-ethylbenzene systems were measured using a sorption apparatus with a quartz spring at 100 and 130°C for mass fractions of ethylbenzene ranging from 0 to 0.17. The mutual diffusion coefficients were correlated with good agreement by the free-volume theory.
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  • 90
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    Polymer Engineering and Science 30 (1990) 
    ISSN: 0032-3888
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
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  • 91
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    Polymer Engineering and Science 30 (1990), S. 485-489 
    ISSN: 0032-3888
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Polymers were prepared from saturated (HMDS) and unsaturated (AN) monomers in a radio frequency discharge (plasma). The effect of selected parameters such as electrode type (such as Cu, Zn, Ni, Al), reactor type, and substrate temperature (other parameters constant) on chemical structure and the rate of polymer deposition was examined by Fourier transform infrared (FTIR) spectroscopy. Differences in the nature of electrodes and reactor types were found to yield similar plasma products with similar rates of deposition for two monomers. In the tubular reactor used, deposition rates were observed to decrease with increasing substrate temperatures showing different dependencies on temperature for the monomers tested.
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  • 92
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    Polymer Engineering and Science 30 (1990), S. 469-475 
    ISSN: 0032-3888
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: The dielectric and magnetic properties of polystyrene composites containing barium or nickel-zinc ferrites were studied as function of the ferrite concentration and field frequency. The composites were prepared by methods yielding a random distribution of the ferrite particles or segregated structures. Barium ferrite-poly-styrene composites exhibited a typical insulator behavior, and only above 60% ferrite were high values of the dielectric properties noted at the lower frequencies, decreasing gradually with frequency to the low values typical of the higher frequencies. The mode of barium ferrite particle distribution did not affect the dielectric properties. The nickel-zinc ferrite systems demonstrated a conductor type behavior. An apparent insulator-conductor transition was observed, having lower values for segregated than for random distributions. The magnetic permability of barium ferrite-polystyrene composites above 10% ferrite increases with the ferrite concentration, whereas the magnetic dissipation factor steeply increases with concentration above 40% ferrite.
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  • 93
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    Polymer Engineering and Science 30 (1990), S. 493-510 
    ISSN: 0032-3888
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Morphology and properties of polymer alloys can be controlled by thermody-namlcally reversible (structure freeze-in) or irreversible (structure lock-in) processes by simultaneously manipulating miscibility, mechanisms of phase separation, glass transition temperature (structural relaxation), and cure kinetics of polymer systems. Using phase diagrams consisting of binodal and spinodal curves, the morphology of epoxy/CTBN (carboxyl-terminated butadiene acryloni-trile copolymer) systems can be controlled by the mechanism of nucleation and growth or by spinodal decomposition via simultaneously manipulating the kinetic processes of phase separation and curing reactions. We have found that the particle size of the rubber reinforcement in epoxies is affected by the mechanisms of phase separation. Phase separation by nucleation and growth gives larger rubber particles than the corresponding phase separation by spinodal decomposition. This contrast in the morphology development is the consequence of controlling phase separation through chemorheological behavior. Medication of the phase separation kinetics in epoxy/CTBN systems was extremely effective at altering both morphology and properties of these alloys. This technique offers a means to shift the glass transition temperature of the rubber-rich phase drastically without reducing the glass transition temperature of the epoxy-rich phase significantly. Such control over morphology is the key to ultimately controlling material properties. This morphology manipulation allows us to tailor the mechanical properties of alloy systems.
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  • 94
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    Polymer Engineering and Science 30 (1990), S. 511-518 
    ISSN: 0032-3888
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Blends of polypropylene (PP) and ethylene-propylene rubber (EPR) and blends of polystyrene (PS) and styrene-butadiene rubber (SBR) were prepared in a laboratory-scale internal mixer at various blend compositions and rotor rates. Blend morphology was studied by means of electron microscopy. For each blend pair under the given processing conditions, the phase inversion process occurred progressively with respect to the variation in blend composition; it is within this composition range of phase inversion that dual-phase continuity was observed. In addition, Characteristic torque values of blends were found to deviate negatively from a linear additivity rule; the composition range of maximum deviation from linear additivity corresponded approximately to the composition range where dual-phase continuity was observed. Sperling's predictive scheme was found to yield acceptable (although not completely satisfactory) estimates for compositions of dual-phase continuity in the present systems. It was also observed that partial cross-linking of SBR during the mechanical blending process, as suggested by the appearance of a cure peak in the torque curve and supported by infrared spectroscopic evidence, resulted in morphological features drastically different from those of the uncured blends.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
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  • 95
    Electronic Resource
    Electronic Resource
    Stamford, Conn. [u.a.] : Wiley-Blackwell
    Polymer Engineering and Science 30 (1990), S. 519-526 
    ISSN: 0032-3888
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Blends of an engineering thermoplastic, poly(ethylene terephthalate) (PET), and two liquid crystalline polymers (LCPs) viz., copolyesters of PET and parahydrox-ybenzoic acid (PHB) in 40/60 mole percent (LCP60) and in 20/80 mole percent (LCP80) were prepared. A blend of LCP60 and LCP80 in 50/50 weight percent (LCP60-80) was blended with PET. Both flat films and rods were extruded and their properties examined. The morphology of the films investigated using Scanning Electron Microscopy (SEM) revealed that the LCP phase remained as dispersed droplets in the PET matrix. In spite of the lack of fibrillation in these films, the mechanical properties were enhanced to some extent with a maximum at 10 weight percent of the LCP phase. However, in the case of the rods thin fibrils of the LCP phase of the order of 1 μm in diameter were observed provided the composition of the LCP was 20 weight percent or greater. This success In achieving fibrillation is through to be due to the extensional flow fields present at the entrance of the capillary die and the fact that a short L/D ratio die was used. Differential Scanning Calorimetry (DSC) thermograms of the extruded films indicated that the LCP phase may act as a nucleating agent for the crystallization of PET. Rheology of the blends revealed that the complex viscosity of the blends is not much different from that of pure PET. This is attributed to the partial miscibility of the two components. Based on the DSC results and residence times in the extruder, it is concluded that no significant transesterification reactions appear to have: taken place in the blends. The rheology is studied further with respect to the cooling behavior of the pure components and factors important to the fibrillation of the LCP phase and the formation of in-situ reinforced composites are discussed.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
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  • 96
    Electronic Resource
    Electronic Resource
    Stamford, Conn. [u.a.] : Wiley-Blackwell
    Polymer Engineering and Science 30 (1990), S. 618-618 
    ISSN: 0032-3888
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Type of Medium: Electronic Resource
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  • 97
    ISSN: 0032-3888
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Eight commercial semiconductor grade epoxy compounds that are used to encapsulate 1C (integrated circuit) devices have been evaluated for their ability to minimize the development of thermal stresses which can cause failure during device temperature cycling. Thermal expansion, dynamic modulus and adhesion studies are used to describe the mechanical interaction between the plastic package and the silicon device it surrounds. A “figure of merit” is defined for the development of stress on the 1C device as it is cooled after the packaging process. The stress is shown to be proportional to the product of three terms: (αp-αs) Ep (Tanch-T) where αp and αs are the expansion coefficients for the plastic and silicon, respectively, Ep is the modulus of the epoxy and Tanch is the temperature at which the epoxy becomes anchored to the silicon device during transfer molding. In addition, the importance of good adhesion between the epoxy encapsulant and the silicon device to prevent package cracking has been demonstrated by finite element analysis and a novel adhesion test.
    Additional Material: 16 Ill.
    Type of Medium: Electronic Resource
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  • 98
    Electronic Resource
    Electronic Resource
    Stamford, Conn. [u.a.] : Wiley-Blackwell
    Polymer Engineering and Science 30 (1990), S. 603-608 
    ISSN: 0032-3888
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Covalent grafting of mesogenic chains on carbon fiber surfaces was attempted as part of a study on composite materials containing liquid crystal polymer matrices. Grafting in these composite systems is viewed not only as a mechanism to achieve interfacial bonding but also as an approach to modify the interphase physical structure. The synthetic approach to grafting involved the in-situ polymerization of monomers in the presence of functionalized fibers in order to grow chains covalently attached to the fibers. The chemical mechanism may be viewed as the “transesterification of car boxy lated fibers” with acetylated monomers. The monomers used were pimelic acid, p-acetoxybenzoic acid and diacetoxy hydroquinone which are known to yield upon condensation a chemically aperiodic nematic polymer. Evidence for grafting was obtained from X-ray photoelectron spectroscopy (XPS) and scanning electron microscopy (SEM) analysis on fibers retrieved from composite samples. Interestingly, SEM micrographs of fractured composite specimens containing the mesogen-grafted fibers reveal excellent wetting and interfacial bonding of a liquid crystalline matrix on the carbon surfaces. Based on theoretical considerations for end-adsorbed macromolecules and the nematogenic nature of the grafted chains we infer that dense layers of adsorbed polymer may form at the interfaces studied. From a materials point of view the in situ growth of liquid crystal polymer chains on fibers may offer mechanisms to control composite properties through both bonding and molecular orientation in interfacial regions.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
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  • 99
    ISSN: 0032-3888
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: We present chain structure, phase morphology, and toughness relationships in thermoplastic polymers and polymer/rubber blends. In neat polymers, molecular aspects of craze/yield behavior are controlled by two chain parameters: entanglement density νe and characteristic ratio C∞. The crazing stress is proportional to νe1/2, and the yield stress is proportional to C∞. The dispersed rubber toughens a polymer/rubber blend mainly by promoting energy dissipation of the matrix. The toughening efficiency correlates with the rubber phase morphology and the chain structure of the matrix.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
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  • 100
    Electronic Resource
    Electronic Resource
    Stamford, Conn. [u.a.] : Wiley-Blackwell
    Polymer Engineering and Science 30 (1990), S. 762-768 
    ISSN: 0032-3888
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: The positron annihilation lifetime spectroscopy (PALS) technique is used to measure volume relaxation in physically aged glassy polycarbonate. The relaxation times and activation energy calculated for the isothermal relaxations in the aged polycarbonate are greater than those parameters calculated for unaged polycarbonate. The activation energy of 8.2 kcal/mol in the aged polycarbonate is used to identify the phenyl group motion or the cooperative carbonate-phenyl interaction as the molecular features responsible for the thermally induced open volume relaxations. It is postulated that the open volume relaxation kinetics as measured by PALS can be used as a nondestructive indication of property differences between aged and unaged polycarbonate.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
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