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  • 1965-1969  (419)
  • 1955-1959  (39)
  • Biochemistry and Biotechnology  (391)
  • Insulin  (35)
  • Electron Microscopy  (32)
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  • 1
    Electronic Resource
    Electronic Resource
    Über Wirkungen von N-Amidino-3,5-diamino-6-chloro-pyrazin-carboxamid (Amilorid) auf den Glucosetransport und den Leucineinbau in Proteine des epididymalen Fettgewebes der Ratte (1969)
    Springer
    Naunyn-Schmiedeberg's archives of pharmacology 262 (1969), S. 139-151 
    Details
    ISSN: 1432-1912
    Keywords: Amiloride ; Insulin ; Glucose Transport ; Leucine Incorporation ; Isolated Adipose Tissue ; Amilorid ; Insulin ; Glucosetransport ; Leucineinbau ; isoliertes Fettgewebe
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung 1. Die Aufnahme von Glucose ins isolierte epididymale Fettgewebe und die Bildung von 14CO2 aus [1-14C]-Glucose wurde als Maß von Transportvorgängen, die Incorporation von [1-14C]-Leucin ins Fettgewebsprotein als Maß der Proteinsynthese vor und nach Zusatz von Amilorid in vitro verwendet. 2. Amilorid setzt den Transport durch die Membran sowohl ohne als auch mit Stimulierung durch Insulin halbmaximal in 10−4 M-Konzentration herab. Aus der Kinetik des Transportes kann geschlossen werden, daß Amilorid die maximale Geschwindigkeit des gesamten Vorganges verlangsamt, ohne die Transportkonstante zu verändern. 3. Der Einbau von Leucin ins Fettgewebsprotein wird durch Amilorid in 3 · 10−4 M-Konzentration auf ein Zehntel herabgesetzt. Das Verhalten von [1-14C]-α-Aminoisobuttersäure läßt den Schluß zu, daß der Aminosäuretransport durch die Membran des Fettgewebes nicht beeinflußt wird. 4. Die Wirkungen des Amilorids am Fettgewebe entsprechen im Prinzip denjenigen des Triamterens und des 6-Aminonicotinsäureamids. Ebenso ist allen gemeinsam, daß sie den renalen Natrium- und Kaliumtransport im distalen Abschnitt des Nephrons hemmen. 5. Es ist möglich, daß ein Zusammenhang zwischen den hemmenden Wirkungen dieser Pharmaka auf den Leucin-Einbau in das Fettgewebsprotein und der Störung von Transportvorgängen durch biologische Membranen besteht.
    Notes: Summary 1. The uptake of glucose and the formation of 14CO2 from [1-14C]-glucose were used as a measure of transport processes, and the incorporation of [1-14C]-leucine into the protein of adipose tissue served as a measure of protein synthesis before and after the addition of amiloride to the isolated epididymal adipose tissue of the rat in vitro. 2. In a concentration of 10−4 M, amiloride decreases the glucose transport through tissue membranes by 50% with and without stimulation by insulin. It can be deduced from the kinetics of the transport that amiloride slows down the maximal velocity of the complete process without influencing the transport constant. 3. The incorporation of leucine into adipose tissue protein was diminished to 10% by amiloride in a concentration of 3 × 10−4 M. Experiments with [1-14C]-α-aminoisobutyric acid allow the conclusion that the amino acid transport through the membrane of the adipose tissue remains uninfluenced. 4. The effects of amiloride on the adipose tissue are similar to those of triamterene and of 6-aminonicotinamide. In addition, they all inhibit renal sodium and potassium transport in the distal part of the nephron. 5. It is possible, that there is a connection between the inhibiting effects of these drugs on the incorporation of leucine into the protein of adipose tissue and the disturbance of transport processes through biological membranes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00537655
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  • 2
    Electronic Resource
    Electronic Resource
    Effect of insulin on glucagon enhanced lipolysis in vitro (1969)
    Springer
    Diabetologia 5 (1969), S. 195-197 
    Details
    ISSN: 1432-0428
    Keywords: Insulin ; glucagon ; adipose tissue ; lipolysis ; FFA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Résumé A des concentrations proches de celles qui sont rencontrées dans le plasma humain, le glucagon stimule fortement la lipolyse au niveau de la graisse épididymaire du rat, étudiéein vitro. Les effets de telles concentrations de glucagon sont réduits, voire abolis par l'insuline aux concentrations de 25 et 100μU/ml. Rapprochées de l'effet insulinogénique puissant du glucagon, ces observations peuvent fournir une explication quant au caractère retardé de l'élévation du taux sanguin des acides gras libres observée après injection de glucagonin vivo.
    Abstract: Zusammenfassung Glucagon stimuliert in Konzentrationen, wie sie auch im menschlichen Plasma vorkommen, die Lipolyse im Ratten-Nebenhodenfettgewebein vitro stark. Die Effekte derartiger Glucagonkonzentrationen werden durch Insulin (25–100μE/ml) verringert bis aufgehoben. Unter Berücksichtigung der ausgeprägten Wirkung von Glucagon auf die Insulinfreisetzung können diese Beobachtungen eine Erklärung für die Verzögerung des Anstiegs der freien Fettsäuren im Serum liefern, die man nach Glucagoninjektionenin vivo beobachtet.
    Notes: Summary Glucagon in concentrations similar to those found in human plasma markedly stimulates lipolysis in rat adipose tissuein vitro. The effects of these “physiological” concentrations of glucagon are reduced or abolished by insulin at concentrations of 25 and 100μU/ml. Considering the marked insulinogenic effect of glucagon these observations may provide an explanation for the delayed increase of blood FFA observed after glucagon injectionin vivo.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01213680
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  • 3
    Electronic Resource
    Electronic Resource
    Insulin release by isolated pancreatic islets of the mouse incubated in vitro (1969)
    Springer
    Diabetologia 5 (1969), S. 61-66 
    Details
    ISSN: 1432-0428
    Keywords: Insulin ; islets of Langerhans ; mouse ; collagenase ; glucose ; mannoheptulose ; glucagon ; adrenaline ; phentolamine ; theophylline ; tolbutamide
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Résumé La libération de l'insuline pendant l'incubation des îlots de Langerhans de la souris, isolés après digestion du pancréas par la collagénase, a été étudiée, et l'influence de divers facteurs sur la vitesse de libération a été recherchée. Le glucose à 3.0 mg/ml (glucose élevé) stimulait la libération d'insuline, mais n'avait pas d'effet à 0.6 mg/ml (taux bas). Le mannoheptulose bloquait la stimulation provoquée par du glucose élevé, comme le faisait l'adrénaline. L'effet de l'adrénaline était aboli par la phentolamine, un agent bloquant alpha adrénergique. Le glucagon seul, ainsi qu'en pre'sence d'un taux de glucose bas, stimulait la libération d'insuline, mais non de façon constante avec un taux de glucose élevé. L'adrénaline abolissait la stimulation provoquée par le glucagon. La théophylline stimulait la libération lorsque le glucose était bas, mais beaucoup moins lorsque le glucose était élevé et pas du tout avec le glucagon, quelle que soit la concentration de glucose. Le tolbutamide stimulait la libération d'insuline avec une faible concentration de glucose et cet effet n'était pas inhibé par l'adrénaline. La convenance de cette préparation pour des études sur le métabolisme des cellules insulaires et sa relation avec la sécrét ion d'insuline est discutée.
    Abstract: Zusammenfassung Es wurde die Insulinausschüttung aus Langerhans'schen Inseln von Mäusen, die durch Kollagenase-Behandlung des Pankreas gewonnen worden waren, untersucht und der Einfluß verschiedener Faktoren auf die Freisetzungsgeschwindigkeit überprüft. Eine hohe Glucose-Konzentration von 3.0 mg/ml förderte die Insulin-Ausschüttung, während die niedrige Konzentration von 0.6 mg/ml keine Wirkung ergab. Mannoheptulose und Adrenalin blockierten die Stimulation durch die hohe Glucosekonzentration. Der AdrenalinEffekt konnte durch Phentolamin, eine alpha-Rezeptoren blockierende Substanz, wieder aufgehoben werden. Glucagon führte allein und in Gegenwart der niedrigen Glucosekonzentration zu einer verstärkten Insulininkretion: dies war jedoch bei Kombination mit der hohen Glucosekonzentration nicht konstant der Fall. Adrenalin hob die Stimulierung durch Glucagon auf. Theophyllin führte bei Gegenwart der niedrigen Glucosekonzentration zu einer gesteigerten Ausschüttung, dieser Effekt trat unter der höheren Glucosekonzentration in wesentlich geringerem Umfang und bei Zusatz von Glucagon und einer der beiden verwandten Glucosekonzentrationen überhaupt nicht auf. Bei niedriger Glucosekonzentration stimulierte Tolbutamid die Insulinfreisetzung; dieser Effekt ließ sich durch Adrenalin nicht aufheben. Die Aussagefähigkeit dieses Präparates für Untersuchungen des Inselzell-Stoffwechsels und seiner Beziehungen zur Insulininkretion wird diskutiert.
    Notes: Summary The release of insulin during incubation of mouse islets of Langerhans, isolated after digestion of the pancreas with collagenase, has been studied, and the influence of various factors on the rate of release investigated. Glucose at 3.0 mg/ml (high glucose) stimulated insulin release, but had no effect at 0.6 mg/ml (low glucose). Mannoheptulose blocked the stimulation by high glucose, as did adrenaline. The adrenaline effect was abolished by phentolamine, an alpha-adrenergic blocking agent. Glucagon alone, stimulated insulin release, and also with low glucose, but not consistently with high glucose. Adrenaline abolished the stimulation by glucagon. Theophylline stimulated release with low glucose, much less so with high glucose and not at all with glucagon at either glucose concentration. Tolbutamide stimulated release with low glucose, and this effect was not inhibited by adrenaline. The suitability of this preparation for studies of islet cell metabolism and its relationship to secretion of insulin is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01211999
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  • 4
    Electronic Resource
    Electronic Resource
    Insulin in bile (1969)
    Springer
    Diabetologia 5 (1969), S. 304-308 
    Details
    ISSN: 1432-0428
    Keywords: Insulin ; bile ; rabbit ; 125I-insulin ; glucose ; galactose ; fructose ; tolbutamide ; phenformin ; liver
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Résumé On trouve de l'insuline immunoréactive dans la bile hépatique de lapins normaux. Moins de 1% d'une dose injectée d'insuline bovine atteint la bile. Quand de l'insuline bovine 125I, seule ou liée à l'anticorps, est administrée par voie intraveineuse, seulement 40% de la radioactivité retrouvée dans la bile est précipitable avec l'acide trichloracétique et moins de 10% réagit avec le sérum anti-insuline de cobaye. Le glucose, le fructose le galactose, le tolbutamide et la phenformine provoquent tous une élévation de l'insuline dans la bile, qui atteint son maximum 40 à 50 min après l'injection. L'alloxane atténue ou abolie ces réponses.
    Abstract: Zusammenfassung Immunoreaktives Insulin wird in der Leber-Galle normaler Kaninchen gefunden. Weniger als 1% einer injizierten Dosis von Rinder-Insulin erreicht die Galle. Wenn man reines oder an Antikörper gebundenes 125I-Rinderinsulin intravenös verabreicht, lassen sich mit Hilfe von Trichloressigsäure nur 40% der Radioaktivität ausfällen, die in der Galle gefunden wird, und weniger als 10% reagieren mit Meerschweinchen-Anti-Insulinserum. Glucose, Fructose, Galaktose, Tolbutamide und Phenformin verursachen ein Ansteigen von Insulin in der Galle, welches ein Maximum innerhalb von 40 bis 50 Min. nach der Injektion erreicht. Alloxan vermindert oder hebt diese Effekte auf.
    Notes: Summary Immuno-reactive insulin is found in the hepatic bile of normal rabbits. Less than 1% of an injected dose of bovine insulin reached the bile. When 125I-bovine insulin alone or complexed with antibody was given intravenously, only 40% of the radioactivity recovered in the bile was precipitable with trichloracetic acid, and less than 10% reacted with guinea pig anti-insulin serum. Glucose, fructose, galactose, tolbutamide and phenformin all caused an elevation in bile insulin, which reached a maximum 40 to 50 min after the injection. Alloxan attenuated or abolished these responses.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00452903
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  • 5
    Electronic Resource
    Electronic Resource
    Experimentelle Untersuchungen über die blutzuckersenkende Wirkung von Rinder-Proinsulin (1969)
    Springer
    Diabetologia 5 (1969), S. 325-330 
    Details
    ISSN: 1432-0428
    Keywords: Insulin ; proinsulin ; blood sugar ; mice ; rats ; pancreatectomy ; nephrectomy ; hepatectomy (partial) alloxan diabetes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Résumé Chez les rats et les souris le taux du sucre sanguin est abaissé par une proinsuline obtenue à partir de l'insuline bovine (Schmidt et Arens [6]). Chez les rats alimentés et les souris à jeun, le rapport d'activité proinsuline: insuline est de 1∶4, chez les rats à jeun de 1∶2, dans le cas de dose équimolaire et en mesurant l'abaissement maximum du sucre sanguin. L'abaissement du sucre sanguin évolue de façon plus lente après proinsuline qu'après insuline. La proinsuline, incubée in vitro avec la trypsine, agit quantitativement et au point de vue temps, comme l'insuline. Chez les rats pancréatectomisés, chez les rats néphrectomisés ainsi que chez ceux hépatectomisés aux deux tiers, l'action de la proinsuline est la même que chez les animaux normaux. Les souris rendues diabétiques par l'alloxane répondent également à la proinsuline comme les souris intactes. L'action hypoglycémiante de la proinsuline se manifeste de façon retardée probablement parce que l'insuline active doit tout d'abord se former par un processus protéolytique dépendant du facteur temps. Ceci pourrait être aussi la raison pour laquelle, Rubenstein et coll. [5] signalent l'absence d'effet de la proinsuline dans le test de convulsion chez la souris.
    Abstract: Zusammenfassung An Ratten und Mäusen wird der Blutzucker durch ein aus Rinderinsulin gewonnenes Proinsulin (Schmidt und Arens [6]) gesenkt. Bei gefütterten Ratten und nüchternen Mäusen ist das WirkungsVerhältnis Proinsulin: Insulin = 1∶4, bei nüchternen Ratten 1∶2, wenn äquiznolar dosiert und das Maximum der Blutzuckersenkung gewertet wird. Die Blutzuckersenkung verläuft nach Proinsulin protrahierter als nach Insulin. Proinsulin, welches in vitro mit Trypsin inkubiert wurde, wirkt quantitativ und zeitlich wie Insulin. Bei pankreatektomierten, bei nephrektomierten und bei zweidrittelhepatektomierten Ratten wirkt Proinsulin ebenso wie an Normaltieren. Auch alloxandidbetische Mäuse sprechen auf Proinsulin wie intakte Mäuse an. Die hypoglykämische Wirkung des Proinsulins tritt wahrscheinlich deshalb verzögert ein, weil das aktive Insulin in einem zeitabhängigen proteolytischen Prozeß erst entstehen muß. Dies könnte auch die Ursache für eine von Rubenstein et al. [5] zitierte, fehlende Wirkung von Proinsulin im Mäusekrampftest sein.
    Notes: Summary Proinsulin obtained from bovine insulin (Schmidt and Arens [6]) lowered the blood sugar level of rats and mice. The activity ratio proinsulin: insulin was 1∶4 in fed rats and fasted mice, and 1∶2 in fasted rats, based on an equimolar dosage and measuring the effect in terms of maximum lowering of the blood sugar value. After proinsulin the decrease of the blood sugar was more protracted than after insulin. Proinsulin which was incubated with trypsin in vitro, resembled insulin in degree and onset of action. In pancreatectomized, nephrectomized and two-thirds hepatectomized rats proinsulin acted as in normal animals. Alloxan-diabetic mice responded to proinsulin like intact mice. The reason why the onset of the hypoglycaemic action of proinsulin was delayed is probably due to the fact that the active insulin must first be produced by a time-dependent proteolytic process. This may also be the cause of the ineffectiveness of proinsulin in the mouse convulsion test which was quoted by Rubenstein et al. [5].
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00452907
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  • 6
    Electronic Resource
    Electronic Resource
    Localization of insulin in mouse tissues using fluorescence microscopy and light microscope and high resolution autoradiography. (1969)
    Springer
    Diabetologia 5 (1969), S. 67-78 
    Details
    ISSN: 1432-0428
    Keywords: Insulin ; mouse tissues ; kidney fluorescence microscopy ; microscopy ; autoradiography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Résumé La localisation d'insuline marquée avec I125 ou avec de l'isothiocyanate de fluorescéine a été étudiée au moyen d'autohistoradiographie au microscope optique, au microscope électronique et au moyen de microscopie à fluorescence dans les tissus de souris à jeun et de souris alimentées normalement. L'hormone fluorescente a été trouvée dans les cellules des tubules proximaux du rein de souris à jeun seulement, tandis que l'hormone radioactive a été repérée aussi dans le rein de souris alimentées, bien qu'en quantité inférieure à celle trouvée chez les souris à jeun. Au microscope électronique la radioactivité est présente dans le rein dans la bordure en brosse, les vacuoles et les mitochondries apicales et le noyau, mais pas dans l'appareil de Golgi ou dans les lysosomes. —L'hormone radioactive est aussi présente dans les autres tissus mais sans différence quantitative entre les souris à jeun et les souris alimentées. Les auteurs concluent que le procédé de ré-absorption de l'insuline par les cellules des tubules proximaux du rein est spécifique: l'hormone n'est pas dégradée de la même façon que les autres protéines mais probablement stockée afin de contrôler les processus métaboliques relevant des organelles subcellulaires dans lesquelles elle se trouve.
    Abstract: Zusammenfassung Gefütterte und fastende Mäuse erhielten fluoreszierendes oder mit125J markiertes Insulin i.v. Die Lokalisation des Insulins in den Geweben wurde untersucht. Nur bei Tieren im Hungerzustand fand sich das fluoreszierende Hormon in den Nieren, während sich das radioaktive Insulin bei gefütterten und fastenden Tieren in den proximalen Tubulusschlingen der Nieren nachweisen ließ. Die fastenden Tiere schienen dabei mehr Radioaktivität aufzuweisen. Wurden die Nieren unter Verwendung von Techniken mit hohem Auflösungsvermögen untersucht, so fand sich die Radioaktivität im Bürstensaum, in den apikalen Vakuolen und Mitochondrien und dem Kern, jedoch nicht im Golgi-Apparat oder den Lysosomen. Alle anderen Gewebe enthielten Hormon-Radioaktivität, aber weder ihre Menge noch ihre Lokalisation unterschieden sich bei gefütterten und fastenden Tieren. Es wird gefolgert, daß der Prozeß der Insulinabsorption durch die Zellen der proximalen Tubulusschlingen spezifisch ist und daß das Hormon nicht in ähnlicher Weise wie andere Eiweißkörper abgebaut wird. Wahrscheinlich kommt es über eine Speicherung zu einer Steuerung der entsprechenden Stoffwechselprozesse in den subzellulären Organellen, in denen das Hormon sich befindet.
    Notes: Summary Starved and fed mice were injected intravenously with either fluorescent or125I-insulin and the localization of the hormone was investigated in various tissues. The fluorescent hormone was found in the kidney of starved animals only, whereas the radioactive hormone was found in the proximal convoluted tubule cells of the kidney of both fed and fasted animals, although the latter group appeared to contain more radioactivity than the first. With high resolution techniques the radioactivity in the kidney was found in the brush border, the apical vacuoles, the apical mitochondria and the nucleus, and never in the Golgi apparatus or the lysosomes. All other tissues contained radioactivity due to the hormone, but no difference in either the amount or the localization was found between fed an starved animals. It is concluded that the process of absorption of insulin by the cells of the proximal convoluted tubules is specific and that the hormone is not degraded in a way similar to other proteins, but is likely to be stored and to control cellular metabolic processes from its sites of localization.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01212000
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  • 7
    Electronic Resource
    Electronic Resource
    The stimulatory effect by insulin on the incorporation of32P radioactive inorganic phosphate into intracellular inorganic phosphate, adenine nucleotides and guanine nucleotides of the intact isolated rat diaphragm (1969)
    Springer
    Diabetologia 5 (1969), S. 79-87 
    Details
    ISSN: 1432-0428
    Keywords: Insulin ; 32P incorporation ; adenine nucleotides ; guanine nucleotides ; Na+K+ ATP-ase ; ouabain
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Résumé Le but de ces investigations a été de contribuer à la compréhension du mécanisme d'action de l'insuline, en provoquant un turn-over accru du phosphate inorganique marqué au32P, en ATP et autres mononucléotides, dans le diaphragme du rat. Pendant l'incubation du diaphragme intact de rat dans un milieu bicarbonaté de Krebs Ringer, sans addition de substrat, on n'a pas observé d'effet de l'insuline sur le contenu en adénine-nucléotides, en guanine-nucléotides, y compris le 3′5′ AMP cyclique et le 3′5′ GMP cyclique. Quand du phosphate inorganique radioactif marqué au32P était présent dans le milieu, l'insuline augmentait l'activité spécifique du phosphate inorganique intracellulaire et provoquait une incorporation accrue du32P dans l'ATP et le GTP. L'effet sur l'ADP était moins prononcé. Il a été montré que ces effets de l'insuline dépendent de la composition ionique du milieu et du transport membranaire des ions. L'augmentation due à l'insuline de l'activité spécifique du phosphate inorganique intracellulaire et du marquage accru au32P de l'ATP (et du GTP) était en grande partie inhibée par l'ouabaïne. Pendant l'incubation dans les milieux où le chlorure de sodium a été remplacé isoosmotiquement par le chlorure de potassium ou de choline, les effets de l'insuline décrits ci-dessus étaient abolis. Les résultats indiquent que le marquage accru de l'ATP (et du GTP) provoqué par l'insuline, peut être en premier lieu attribué à des phénomènes survenant au niveau de la membrane cellulaire. L'augmentation de l'activité spécifique du phosphate inorganique intracellulaire en présence d'insuline, peut s'expliquer par une captation accrue du phosphate inorganique marqué au32P, à partir du milieu. Apparemment le marquage accru au32P de l'ATP (et du GTP) est une conséquence secondaire. On suggère que cet effet de l'insuline est d'abord en rapport avec un effet sur les enzymes de la membrane, en particulier sur l'ATP-ase activée par Na+, K+ et Mg2+.
    Abstract: Zusammenfassung Ziel dieser Untersuchungen war es, zu einem besseren Verständnis der stimulierenden Wirkung von Insulin auf den Einbau von anorganischem32P Phosphat in ATP und andere Mononucleotide im Rattenzwerchfell beizutragen. Während der Inkubation intakter Rattenzwerchfelle in Krebs-Ringer-Bicarbonat Puffer ohne Substratzusatz konnte kein Insulineffekt auf den Gehalt an Adenonucleotiden und Guaninnucleotiden, sowie von cyclischem 3′,5′ AMP und cyclischem 3′,5′ GMP beobachtet werden. In Gegenwart von anorganischem32P Phosphat steigerte Insulin die spezifische Aktivität von intrazellulärem anorganischen Phosphat und bewirkte einen verstärkten Einbau von32P in ATP und GTP. Die Wirkung auf ADP war weniger ausgeprägt. Es konnte gezeigt werden, daß diese Insulineffekte von dem IonenGehalt des Mediums und dem Membrantransport der Ionen abhängen. Die Steigerung der spezifischen Aktivität des anorganischen Phosphates und der Markierung von ATP (und GTP) mit32P ließ sich weitgehend durch Ouabain wieder aufheben. Bei Inkubation in Pufferlösungen, die statt NaCl isoosmolare Mengen von KCl oder Cholinchlorid enthielten, waren die oben erwähnten Insulineffekte nicht mehr nachweisbar. Die Resultate sprechen dafür, daß die verstärkte Markierung von ATP (und GTP) unter Insulin in erster Linie auf Vorgängen an der Zellmembran beruht. Die erhöhte spezifische Aktivität des intrazellulären anorganischen Phosphates in Gegenwart von Insulin läßt sich durch einen verstärkten Einstrom von32P Phosphat aus dem Medium erklären. Augenscheinlich kommt es dadurch sekundär zu einer gesteigerten32P Markierung von ATP (und GTP). Wir möchten annehmen, daß dieser Insulineffekt vorwiegend über die Membranenzyme erfolgt und sich vor allem auf die Na+, K+ und Mg++ aktivierbare ATPase erstreckt.
    Notes: Summary The aim of these investigations has been to contribute to an understanding of the mechanism of action of insulin in promoting increased turn over of32P inorganic phosphate into ATP and other mononucleotides in the rat diaphragm. During incubation of the intact rat diaphragm in Krebs Ringer bicarbonate medium without addition of substrate, no effect of insulin on the content of adenine nucleotides and guanine nucleotides including cyclic 3′,5′ AMP and cyclic 3′,5′ GMP has been observed. When32P radioactive inorganic phosphate was present in the medium, insulin increased the specific activity of intracellular inorganic phosphate and promoted an increased incorporation of32P into ATP and GTP. The effect on ADP was less pronounced. It has been shown that these effects of insulin depend upon the ionic composition of the medium and on ion membrane-transport. The insulin-promoted increase of specific activity of intracellular inorganic phosphate and of increased32P labelling of ATP (and GTP) was to a great extent inhibited by ouabain. During incubation in media where sodium chloride had been iso-osmotically replaced by potassium chloride or choline chloride, the above mentioned effects by insulin were abolished. The results indicate that the increased labelling of ATP (and GTP) promoted by insulin can be primarily attributed to events occuring at the cell membrane. The increased specific activity of intracellular inorganic phosphate in the presence of insulin can be explained by increased uptake of32P inorganic phosphate from the medium. Apparently the increased32P labelling of ATP (and GTP) occur as a secondary consequence. It is suggested that this insulin effect is primarily concerned with an effect on membrane enzymes, particularly the Na+, K+, Mg2+ activated ATP-ase.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01212001
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  • 8
    Electronic Resource
    Electronic Resource
    Inability of galactose to mobilize insulin in galactokinase-deficient individuals (1969)
    Springer
    Diabetologia 5 (1969), S. 143-145 
    Details
    ISSN: 1432-0428
    Keywords: Insulin ; galactose ; galactokinase ; deficiency
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Résumé Chez deux adultes souffrant d'une déficience en galactokinase la charge orale de galactose a été suivie d'une hypergalactosémie prolongée sans aucune élevation de l'insuline immunoréactive plasmatique.
    Abstract: Zusammenfassung Orale Belastung mit Galactose führte bei zwei Erwachsenen mit Galactokinasemangel zu einer prolongierten Hypergalactosämie, aber nicht zu einem Anstieg des immunoreaktiven Insulins im Plasma.
    Notes: Summary Oral galactose loading in two galactokinase-deficient adults produced the expected high and prolonged rise of galactose in peripheral blood, but no rise of circulating immunoreactive insulin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01213671
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  • 9
    Electronic Resource
    Electronic Resource
    Electron microscopic observations in mice infected with Japanese encephalitis (1969)
    Springer
    Acta neuropathologica 13 (1969), S. 169-181 
    Details
    ISSN: 1432-0533
    Keywords: Japanese Encephalitis ; Experimental ; Electron Microscopy ; Virus Particles in Neurons
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Groß- und Kleinhirnrinde, Plexus chorioideus und Rückenmark von Mäusen wurden nach intracerebraler Inoculation einer Hirnemulsion mit Virus der Encephalitis japonica (JEV) elektronenoptisch untersucht, um den Ort der JEV-Replikation zu bostimmen. 72 Std nach der Inoculation bei Beginn der encephalitischen Symptome enthielten 70 bis 80% aller Rindenneurone und Vorderhornzellen viele sphärische Partikel, die meist im zarten endoplasmatischen Reticulum (EPR) und vereinzelt im granulären EPR lokalisiert waren. Die Einzelpartikel zeigten eine gleichförmige Substruktur aus einem elektronendichten zentralen Hof von 25–30 mμ Durchmesser, einer äußeren, weniger elektronendichten Zone und einer äußersten Grenzmembran von 40 mμ Durchmesser. 96 Std nach der Inoculation zeigte das Cytoplasma der Rinden- und Vorderhornneurone sehr viele Vacuolen und Vesiceln. Partikel wurden weit verstreut in den Vacuolen und Vesiceln sowie erstmals im ERP der Sternzellen und Purkinjezellen angetroffen, allerdings in geringerer Zahl. Keine derartigen Partikel wurden in Kontrolltieren und normalen Mäusegruppen angetroffen. Sogenannte eosinophile intranucleäre Einschlüsse in Epithelzellen des Plexus chorioideus zeigten keine derartigen Partikel im Kern oder in den cytoplasmatischen Bläschen. Nachdem keine als JEV identifizierbaren Partikel in Glia- und Endothelzellen nachzuweisen waren, wird angenommen, daß das JEV echt neurotrop ist und sich im EPR der Nervenzellen repliziert.
    Notes: Summary Cerebral and cerebellar cortices, choroid plexus and spinal cord of mice, inoculated intracerebrally with a brain emulsion containing Japanese encephalitis virus (JEV), were studied electronmicroscopically to determine the cell type and the site of JEV replication. 72 hours after inoculation, when the mice began to show encephalitic symptoms, 70 to 80% of all cortical neurons and anterior horn cells contained many spherical particles mostly located in the smooth endoplasmic reticulum and a few in the granular endoplasmic reticulum. The individual particles demonstrated an uniform substructure consisting of an electron dense central core of 25–30 mμ diameter, an outer less electron dense zone and an outermost limiting membrane of 40 mμ diameter. 96 hours after inoculation, the cytoplasm of cortical neurons and anterior horn cells was observed to contain very many vacuoles and vesicles. Particles were found widely scattered throughout the vacuoles and vesicles, and were observed for the first time in the endoplasmic reticulum of the stellate neurons and in Purkinje cells, though fewer. No such particles were observed in control and normal mouse groups. So-called eosinophic intranuclear inclusions of epithelial cells of choroid plexus failed to show any particles in their nuclei or cytoplasmic vesicles. Considering that no particulate matter, identifiable as JEV, was identified within any of the glial cells or endothelium in this examination, it was concluded that JEV was really neurotropic and replicates in the endoplasmic reticulum of the neurons.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00687029
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  • 10
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    Electronic Resource
    Granulomatous encephalitis in Whipple's disease (1969)
    Springer
    Acta neuropathologica 13 (1969), S. 1-11 
    Details
    ISSN: 1432-0533
    Keywords: Whipple's Disease ; Granulomatous Encephalitis ; Electron Microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Von 21 Fällen von Whipplescher Krankheit in der Sammlung des Armed Forces Institute of Pathology zeigten 4 Fälle neurologische Symptome mit besonders ausgeprägten Hirnläsionen. Lichtmikroskopisch bestanden die Herde aus knötchenförmigen Anhäufungen von Makrophagen, deren Cytoplasma mit Hämatoxylin-Eosin eine charakteristische schwachblaue und nach Durchführung der Perjodsäure-Schiff-Reaktion eine stark rote Färbung zeigte. Elektronenmikroskopisch fanden sich in den Hirnläsionen Bacillen, die morphologisch den im Darm beschriebenen glichen und die auch dieselben Degenerationserscheinungen aufwiesen. Die Perjodsäure-Schiff-Reaktion färbt Kapselmaterial und Zellwände,die auch nach Untergang der Bacillen noch nachweisbar sind. Diese Beobachtungen werden als weiterer Beweis für die infektiöse Ätiologie der Whippleschen Krankheit angeführt.
    Notes: Summary Among the 21 autopsied cases of Whipple's disease on file at the Armed Forces Institute of Pathology, 4 had neurological symptoms and prominent involvement of the central nervous system, By light microscopy the lesions were composed of nodular aggregates of macrophages having cytoplasm that stained a distinctive pale blue with hematoxylin-eosin and bright red with the periodic acid-Schiff technique. By electron microscopy the cerebral lesions revealed bacilli morphologically identical to and undergoing the same sequence of degenerative changes as those observed in the intestine. The periodic acid-Schiff reaction stains the walls and the capsular material that persists even after intact organisms disappear. These observations further support the infectious nature of Whipple's disease.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00686137
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  • 11
    Electronic Resource
    Electronic Resource
    Ultrastructural and histochemical studies of cortical biopsies in subacute dementia (1969)
    Springer
    Acta neuropathologica 13 (1969), S. 43-55 
    Details
    ISSN: 1432-0533
    Keywords: Subacute Dementia ; Jakob-Creutzfeldt Disease ; Brain Biopsy ; Histochemistry ; Electron Microscopy ; Basement Membrane ; Astroglia Dilatation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Die elektronenoptische und histochemische Untersuchung von Biopsiematerial bei zwei Fällen subakuter Demenz ergab Neurone mit reichlich Lipofuscin-Depots sowie erhöhter saurer Phosphatase-Aktivität. Obwohl diese Veränderungen eine Nervenzellerkrankung anzeigen dürften, werden sie wegen ihrer Ähnlichkeit sowie wegen des Vorliegens anderer, spezifischerer Zellveränderungen als Sekundärphänomene gedeutet. Im ersten Fall wird als primäre Läsion eine Verdickung der Basalmembran der Gefäße, im zweiten Fall eine Astrogliaschwellung angenommen. Da diese Strukturen wichtige Transportfunktionen haben dürften, könnten diese Anomalien leicht die neuronale Versorgung stören und dadurch die neuronalen Läsionen verursachen. Die Bedeutung des Glykogens in diesen Strukturen wird diskutiert.
    Notes: Summary The electron microscopic and histochemical findings in biopsies of two cases of subacute dementia have revealed neurons containing numerous lipofuscin bodies and increased acid phosphatase. Although these changes are considered to indicate neuron disease, they are believed to represent a secondary process because of their similarity and because other more distinctive cell alterations are present. In the first case, the primary abnormality is believed to be thickening of the vascular basement membrane, while in the second case, astroglial dilatation is considered a primary change. Since these structures appear to have important transport functions, these abnormalities could easily impair the nutrient supply to neurons and, in this way, produce the neuronal alteration. The significance of glycogen in these tissues is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00686140
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  • 12
    Electronic Resource
    Electronic Resource
    Ultrastructural study of a nerve biopsy from a case of early infantile chronic neuropathy (1969)
    Springer
    Acta neuropathologica 13 (1969), S. 131-142 
    Details
    ISSN: 1432-0533
    Keywords: Neuropathy, Chronical ; Hypertrophic Neuritis ; Schwann-Cell ; Onion Bulb ; Electron Microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Die wesentlichen Veränderungen bei dem mitgeteilten Fall von chronischer frühinfantiler Neuropathie bestehen in einer Modifikation der Schwann-Zellen, die zur Bildung eines konzentrischen Netzwerkes von Lamellen führt. Dieses wird durch eine doppelkonturierte Hülle der Basalmembran um zahlreiche große bemarkte und unbemarkte Fasern gebildet. Die Anomalien der Markscheiden sowie die Häufigkeit von Desmosomen sind bemerkenswert. Diese Besonderheiten unterscheiden diesen Fall von anderen, bisher beschriebenen chronischen Neuropathien. Trotzdem erscheint es derzeit nicht möglich, eine Aussage über die Spezifität der ultrastrukturellen Befunde am untersuchten Nerven zu treffen.
    Notes: Summary The principal lesion in this case of chronic early infantile neuropathy is a modification of the Schwann cell resulting in the formation of a concentric network of lamellae formed by a double-layered sheet of basement membrane around numerous, large myelinated and unmyelinated fibers. The abnormalities in the myelin sheaths and the frequency of desmosomes are noteworthy. These peculiarities distinguish this case from other chronic neuropathies described up to now. Nevertheless, it is impossible to say at present whether the ultrastructural aspect of the nerve studied here is in any way specific.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00687025
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  • 13
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    Electronic Resource
    Structural changes of the central nervous system in swayback (enzootic Ataxia) of lambs (1969)
    Springer
    Acta neuropathologica 12 (1969), S. 307-313 
    Details
    ISSN: 1432-0533
    Keywords: Swayback of Lambs ; Electron Microscopy ; Corpus Callosum ; Myelogenesis ; Axonal Changes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Elektronenmikroskopische Untersuchung des Corpus callosum normaler Lämmer und von solchen mit „Swayback” (enzootische Ataxia) ergab den morphologischen Nachweis normaler Myelinentwicklung in beiden Tiergruppen. Von zwei Zellfortsätzen umgebene Axone wurden häufig beobachtet. Reaktive Veränderungen wurden in Axonen der „Swayback”-Lämmer gefunden. Die Bedeutung dieser Befunde wird besprochen.
    Notes: Summary An electron microscopic study of the corpus callosum of normal and swayback lambs has revealed morphological evidence of normal myelinogenesis in both groups of animals. Axons wrapped by two cell processes were frequently encountered. Reactive changes were found in axons in the swayback lambs. The significance of these findings is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00809127
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  • 14
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    Electronic Resource
    Electron microscopic study of metachromatic leucodystrophy (1969)
    Springer
    Acta neuropathologica 13 (1969), S. 149-156 
    Details
    ISSN: 1432-0533
    Keywords: Electron Microscopy ; Leucodystrophy, Metachromatic ; Enzyme Histochemistry, Electronmicroscopical ; Lysosomes ; Neurochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Résumé Dans la leucodystrophie métachromatique, les lipides anormaux s'accumulent dans les lysosomes des cellules gliales et des phagocytes. Les inclusions lipidiques les moins organisées sont les plus riches en phosphatases acides. Celles dont l'ultrastructure est prismatique ont une activité enzymatique très faible et semblent plus proches de corps résiduels que de lysosomes actifs. Il semble que l'absence d'arylsulfatase lysosomiale induise la persistance des sulfatides accumulés dans les lysosomes alors que les autres lipides provenant de la dégénérescence myélinique, seraient normalement catabolisés. Un remaniement progressif de l'ultrastructure du contenu des lysosomes en est la conséquence.
    Notes: Summary In metachromatic leucodystrophy abnormal lipids are stored within the lysosomes of the glial cells and phagocytes. The lipid inclusions which are the less structured are the richest in acid phosphatases. Those of which the ultrastructure is prismatic show a very low enzymatic activity and are more similar to residual bodies than to active lysosomes. It appears that the lack in lysosomal arylsulfatase leads to an accumulation of the sulfatide moiety of the stored lipids while the other lipids originating from the myelin breakdown, would be normally catabolized. This leads to a progressive transformation of the ultrastructure of the lysosome contents.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00687027
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  • 15
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    Electronic Resource
    Electron histochemical demonstration of gangliosides in normal and Tay-Sachs brain tissue (1969)
    Springer
    Acta neuropathologica 13 (1969), S. 157-168 
    Details
    ISSN: 1432-0533
    Keywords: Gangliosides ; Tay-Sachs Disease ; Brain Subcellular Localization ; Hale Stain ; Electron Microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Die Reaktion von kolloidalem Eisen mit Sialomucinen (Hale stain) wurde zur elektronenmikroskopischen Darstellung der neuraminsäurehaltigen Ganglioside in den subcellulären Fraktionen des Gehirns angewandt. Zunächst wurde mit Hilfe tritiummarkierter Ganglioside überprüft, ob bei der Gewinnung subzellulärer Fraktionen Ganglioside nicht unspezifisch subcellulär adsorbiert werden. Eine nennenswerte Adsorption konnte ausgeschlossen werden. Das Verfahren wurde zuerst auf die aus Meerschweinchengehirnen isolierten Fraktionen angewandt. Da Nervenendigungen, Mitochondrien und Synapsenbläschen Hale-positiv reagierten, dienten Enzyminkubationen mit Neuraminidase und Hyaluronidase zur Differenzierung von anderen Hale-positiven sauren Substanzen. Schließlich wurde die Methode auf die cytoplasmatischen multilamellären Körperchen (MCB) übertragen, die aus Gehirnen an Tay-Sachs'scher Krankheit Verstorbener isoliert wurden. Aus den Versuchen konnte abgeleitet werden, a) daß die Hale-Färbung auch Ganglioside anfärbt, b) daß Ganglioside auf der Oberfläche der Membran von den multilamellären Körperchen und mit hoher Wahrscheinlichkeit auch auf der Membran der Nervenendigungen lokalisiert sind, c) daß die Synapsenbläschen eine positive Reaktion ergeben, die jedoch nicht mit Sicherheit auf vorhandene Ganglioside zurückgeführt werden kann, d) daß die äußere Mitochondrienmembran Hale-positive Substanzen enthält, deren Natur unbekannt ist.
    Notes: Summary The reaction of colloidal ferric oxide with sialomucins (Hale stain) was applied to sialic acid-containing gangliosides in subcellular fractions of the brain, in order to investigate their localization by electron microscopy. Prior to all experiments, a check was made, by means of tritium-labeled ganglioside, to confirm that the ganglioside content in the subcellular particles was not the result of an unspecific adsorption during the isolating procedure. No considerable unspecific adsorption could be registered. Hale stain was first applied to subcellular fractions obtained from guinea-pig brains. Since nerve-endings, mitochondria and synaptic vesicles gave a positive staining reaction, incubations with neuraminidase and hyaluronidase were carried out in order to achieve a differentiation. Finally, the method was applied to the membranous cytoplasmic bodies (MCB) which were isolated from the brain of a patient with Tay-Sachs disease. It was concluded that: a) Hale stain stains gangliosides also, b) gangliosides are localized in the membrane of membranous cytoplasmic bodies and also very probably in the membrane of nerve-endings, c) synaptic vesicles give a Hale-positive reaction which cannot with certainty be attributed to gangliosides, d) the outer membrane of mitochondria contains Hale-positive substances, the nature of which is not known.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00687028
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  • 16
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    Electronic Resource
    Electron microscope study of the rat brain with experimental sodium azide intoxication (1969)
    Springer
    Acta neuropathologica 14 (1969), S. 185-193 
    Details
    ISSN: 1432-0533
    Keywords: Hypoxia ; Sodium Azide ; Electron Microscopy ; Ferritin ; Capillary Permeability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Experimentelle histotoxische Hypoxämie wurde in Ratten durch Natriumacid-Vergiftung erzeugt. Die mikroskopische Untersuchung der Hirne ergab keine Veränderung, aber bei der elektronenmikroskopischen Untersuchung fanden sich als Frühveränderungen in der Hirnrinde und im Subcortex eine Erweiterung der intercellulären Räume und eine Anschwellung der pericapillären Astrocytenfortsätze. Ferritinalbumin-Mischung, die als Indicator des Eiweißtransportes benutzt wurde, zeigte keine Vermehrung der Capillarpermeabilität. Es wird vermutet, daß Natriumacid den Wasserstoffwechsel beeinträchtigt, wobei es eine osmotische Ausgleichstörung im Gehirn erzeugt.
    Notes: Summary Histotoxic hypoxia was experimentally produced in the rat brain by sodium azide intoxication. In spite of the absence of light microscopic alterations, electron micrographs showed the early changes in the cerebral cortex and subcortex which consisted of widening of the intercellular space and swelling of the pericapillary astrocytic end-feet. However, ferritin-albumin mixture, used for the tracer of protein transport, did not show any increase in the capillary permeability. Sodium azide was considered to disturb the water metabolism, producing osmotic unbalance in the brain.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00685298
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  • 17
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    Electronic Resource
    Electron microscopic studies concerning the structural mechanism of the development of mental disturbance in experimental chronic methamphetamine poisoning (1969)
    Springer
    Acta neuropathologica 14 (1969), S. 215-225 
    Details
    ISSN: 1432-0533
    Keywords: Methamphetamine Poisoning (Artificial Psychosis) ; Synapses ; Nerve Conduction and Transmission ; Electron Microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Amphetaminchlorid in Mengen von 1 mg pro kg pro Tag wurde 8 männlichen Meerschweinchen ungefähr 1 Jahr lang täglich injiziert, um eine chronische Amphetaminvergiftung zu erzeugen. Bei jedem vergifteten Tier wurde die Feinstruktur des Gehirns elektronenmikroskopisch untersucht. Die Befunde waren folgende: 1. Verschmelzung der Membranen zwischen Axonen in den Nervenendigungen sowie zwischen Axonen und Dendriten wurden in der Großhirnrinde, im limbischen System, im Thalamus und Hypothalmus beobachtet; ferner wurde eine sich vom Cytoplasma bis zur Nervenendigung erstreckende Membranstruktur in diesen Hirnbereichen festgestellt. 2. Im Frontal-, Parietal-, Temporal- und Occipitalhirn sowie im limbischen System fanden sich vermehrt membranbegrenzte Vesikel im Cytoplasma der Nervenzellen und verdicktes und vermehrtes ER in den Nervenfasern. In Anbetracht klinischer Symptome der Patienten mit Amphetaminvergiftung wurde angenommen, daß die Verschmelzung von Membranen zwischen Nervenfortsätzen und das Auftreten der Membranstrukturen in allen Teilen des Nervenzellcytoplasmas vom Perikaryon bis zur Nervenendigung die wichtigsten Befunde sind, die darauf hindeuten, daß die Vergiftungsvorgänge einen wesentlichen Einfluß auf das Leitungssystem der Nerven ausüben können.
    Notes: Summary In 8 male albino strain guinea pigs, 1 mg/kg of methamphetamine HCl was injected daily for 7 months to 1 year to produce chronic methamphetamine intoxication and to study the fine structure of the brain electron microscopically. The following results were obtained: 1. Coalescence of membranes, between axons at the nerve endings and between axons and dendrites, was found in the cortex of the cerebrum and limbic system, in the thalamus and hypothalamus. Membranous structures were found in all portions of the cytoplasm of the nerve cells. 2. Within the cytoplasm of the nerve cell, coated vesicles were increased in number with hypertrophy and increase of endoplasmic reticulum within nerve fibres in the frontal, parietal, temporal and occipital regions, and in the limbic system. In view of these findings, as well as the clinical neurological symptoms in patients with methamphetamine poisoning, the coalescence of membranes between nerve processes and the appearance of membranous structures in all portions of nerve cell cytoplasm from body to nerve endings appear to represent the most important findings, they probably produce a profound disturbance in the system of nerve conduction and transmission.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00685301
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  • 18
    Electronic Resource
    Electronic Resource
    Bildung von markscheidenähnlichen Spiralstrukturen durch lamelläre Astrocytenausläufer innerhalb subpialer und perivasculärer Gliosen des Säugetiergehirns (1969)
    Springer
    Acta neuropathologica 12 (1969), S. 98-102 
    Details
    ISSN: 1432-0533
    Keywords: Marginal Siderosis ; Electron Microscopy ; Myelin-Sheath-Like Structures ; Plaques Fibromyéliniques ; Gliosis, Perivascular, Subpial
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Bei Kaninchen mit einer experimentell erzeugten Randzonensiderose des Zentralnervensystems konnten elektronenmikroskopisch in der Großhirnrinde eigentümliche Spiralstrukturen beobachtet werden. Diese Spiralen fanden sich vornehmlich im Bereich von subpialen und perivasculären Gliosen vor. Sie bestanden aus äußerst dünnen lamellären Astrocytenausläufern, welche um andere astrocytäre Zellfortsätze oder um Dendriten aufgewickelt waren. Die spiralisierten Astrocytenausläufer wiesen in ihrem Inneren feine Filamente mit einer ziemlich regelmäßigen Anordnung auf. In radiärer Richtung ließen die astrocytären Lamellenspiralen eine periodische Schichtung erkennen, welche etwas an die von echten Markscheiden erinnerte. Es wird in Betracht gezogen, daß sie das submikroskopische Äquivalent der sog. Plaques fibromyéliniques darstellen.
    Notes: Summary Peculiar spiral structures could be observed by electron microscopy in the cerebral cortex of rabbits with an experimentally produced marginal siderosis of the central nervous system. These spirals were found predominantly within areas of subpial and perivascular gliosis. They consisted of extremely thin lamellar astrocyte extensions winding around other astroglial processes or dendrites. The spirally coiled astrocyte extensions exhibited within their interior fine filaments having a rather regular arrangement. In the radial direction, the astrocytic lamellae spirals showed a periodic stratification somewhat resembling that of true myelin-sheaths. They were considered to represent the submicroscopic equivalent of the socalled plaques fibromyéliniques.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00685315
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  • 19
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    Electronic Resource
    Changes at the nodes of ranvier during wallerian degeneration: an electron microscope study (1969)
    Springer
    Acta neuropathologica 14 (1969), S. 237-249 
    Details
    ISSN: 1432-0533
    Keywords: Electron Microscopy ; Wallerian Degeneration ; Nodal Changes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Es wird über ultrastrukturelle Veränderungen in den Ranvierschen Knoten des N.suralis der Ratte im Laufe der Wallerschen Degeneration berichtet. Die Untersuchungen erfolgten 12 und 120 Std nach einer örtlichen Quetschungsverletzung. Die ersten bemerkbaren Veränderungen finden sich im Axon. Nodale und paranodale Anhäufungen von Mitochondrien, multivesikulären und lamellären Körpern, wie auch kleinen blasen- und röhrenartigen Bildungen sind teilweise in den Knoten sichtbar und am deutlichsten nach 24–36 Std erkennbar. Gleichzeitig erfolgt eine Aufsplitterung der Neurofilamente und Neurotubuli, die ihre Liniengestaltung verlieren und sich zusammenballen. Die Zone der erhöhten Dichte gerade unterhalb des nodalen Axolemmas bleibt erhalten. Veränderungen im Myelin beginnen etwas später und bestehen in einem vesikulären Verfall der Endomyelinlamellen und einer Trennung der Endomyelinschlaufen vom Axolemma durch Schwannzellenfortsätze. Dieser Vorgang schien mit einem Zurückziehen des Myelins vom Knoten im Zusammenhang zu stehen. Schwannzellenfortsätze erstrecken sich auch so weit, daß sie das nodale Axon bedecken, wobei sie die nodalen Schwannzellenfortsätze vom Axolemma trennen. Das Endstadium ist die Unterbrechung des nodalen Axons und die Verschmelzung der Myelinenden als Teil der Ovoidbildung.
    Notes: Summary Observations are reported on the ultrastructural alterations at the nodes of Ranvier in the rat sural nerve during the course of Wallerian degeneration. These were examined between 12 and 120 hours after a localized crush injury. The earliest detectable changes are in the axon. Nodal and paranodal accumulations of mitochondria, multivesicular bodies, lamellar bodies and small vesicular and tubular profiles are seen at a proportion of the nodes and are most evident at 24–36 hours. Concomitantly with this, the neurofilaments and neurotubulus fragment, lose their alignment and clump together. The zone of increased density just beneath the nodal axolemma is preserved. Changes in the myelin begin slightly later and consist of vesicular breakdown of the terminal myelin lamellae, and separation of the terminal myelin loops from the axolemma by Schwann cell processes. The latter event appeared to be associated with retraction of the myelin from the node. Schwann cell processes also extend to cover the nodal axon, separating the Schwann cell nodal processes from the axolemma. The final stage is the interruption of the nodal axon and the fusion of the ends of the myelin as part of ovoid formation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00685303
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  • 20
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    Electronic Resource
    Intranucleäre stabförmige Gebilde bei einem Oligodendrogliom (1969)
    Springer
    Acta neuropathologica 13 (1969), S. 289-293 
    Details
    ISSN: 1432-0533
    Keywords: Oligodendroglioma ; Nuclear Inclusions ; Electron Microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Es wird die Ultrastruktur von intranucleären stabförmigen Gebilden bei einem Oligodendrogliom beschrieben.
    Notes: Summary Ultrastructure of intranuclear rodlets in an oligodendroglioma is described.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00690649
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  • 21
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    Electronic Resource
    Familial spongy degeneration of the central nervous system (Van Bogaert-Bertrand disease) (1969)
    Springer
    Acta neuropathologica 12 (1969), S. 103-115 
    Details
    ISSN: 1432-0533
    Keywords: Spongy Degeneration of the CNS ; Van Bogaert-Bertrand-Disease ; Electron Microscopy ; Astrocytic Mitochondria ; Abnormal Cerebral Edema
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Es wird über licht- und elektronenoptische Untersuchungen an Muskel-und Hirnbiopsien eines Falles von spongiöser Degeneration des ZNS berichtet. Die in der grauen und weißen Hirnsubstanz enthaltenen Vacuolen entsprechen a) Spalten in den Markscheiden infolge Aufsplitterung an der intraperiodischen Linie und b) geschwollenen Astrocytenperikaryen und-fortsätzen. Ungewöhnliche Mitochondrien mit Gehalt an kristallinem Material fanden sich nur in Astrocyten. Die ultrastrukturellen Befunde entsprechen denen des Hirnödems. Es wird angenommen, daß die Astroglia eine primäre Rolle in der Flüssigkeitsansammlung spielt, während die Markscheidenschwellung als eine Sekundärläsion aufgefaßt wird. Die mögliche Bedeutung abnormer Astrocyten-Mitochondrien wird diskutiert.
    Notes: Summary Light and electron microscopy study of skeletal muscle and cerebral biopsies from a case of spongy degeneration of central nervous system is reported. The multiple vacuoles present in cerebral gray and white matter correspond to (a) clefts within myelin sheaths resulting from splitting at the intraperiod line and (b) swollen astrocytic perikarya and processes. Unusual mitochondria containing crystalline-like material were observed only in astrocytes. The ultrastructural findings are consistent with cerebral edema. It is suggested that the astrocytes play a primary role in the fluid accumulation while the myelin swelling is a secondary lesion. The possible role of the abnormal astrocytic mitochondria is discussed.
    Type of Medium: Electronic Resource
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    Electronic Resource
    Abnormal sarcolemmal nuclei encountered in several cases of dystrophia myotonica (1969)
    Springer
    Acta neuropathologica 12 (1969), S. 183-188 
    Details
    ISSN: 1432-0533
    Keywords: Dystrophia myotonica ; Electron Microscopy ; Sarcolemmal Nuclei ; Myofibrillar Material
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung In fünf Fällen von Dystrophia myotonica zeigte die elektronenoptische Untersuchung von bioptisch entnommenem Material zentrale und subsarkolemmale Kerne, die komplexe vacuoläre Areale um myofibrilläres Material enthalten. Diese bisher nicht beobachtete Veränderung könnte durch Verlagerung von myofibrillärem Material in den Kern während seiner Wanderung in das Faserzentrum durch Invagination, aber nicht Durchbrechung der Kernmembran bedingt sein.
    Notes: Summary In five cases of dystrophia myotonica electron microscope preparations of muscle taken at biopsy showed both centrally placed and subsarcolemmal nuclei containing complex vacuolar areas enclosing myofibrillar material. It is suggested that this appearence, which has not been seen in any other condition, may be due to the forcing of myofibrillar material into the nucleus during its migration to the centre of the fibre invaginating but not breaching its membrane.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00692505
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  • 23
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    Electronic Resource
    Electron microscopic studies on the cerebral lesions of rats in experimental carbon monoxide poisoning (1969)
    Springer
    Acta neuropathologica 14 (1969), S. 118-125 
    Details
    ISSN: 1432-0533
    Keywords: Carbon Monoxide Poisoning ; Electron Microscopy ; Myelin Disintegration ; Cerebral Anoxia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Nach experimenteller CO-Vergiftung fanden sich schwerere und ausgedehntere Läsionen in der weißen als in der grauen Hirnsubstanz. Zwischen akuter und chronischer CO-Vergiftung ergaben sich keine wesentlichen qualitativen Unterschiede der Hirnveränderungen. Die Läsionen nach chronischer Intoxikation waren jedoch schwerer als nach akuter Vergiftung. Die Nervenzellen zeigten leicht dilatiertes oder fragmentiertes EPR und Golgi-Komplexe, doch boten die Mitochondrien meist keine pathologischen Veränderungen. Bei chronischen Fällen fand sich gelegentlich eine deutliche Schwellung des EPR von Oligodendrogliazellen. Die Capillaren blieben intakt und die perivasalen Astrogliaendfüßchen waren nicht geschwollen. Im Mark kam es zu teilweisem Ausfall der normalen Markscheidenbestandteile. Die Marklamellen zeigten mäßige Exfoliation sowie eine Umwandlung in homogene oder strukturlose Substanz. Bemarkte Axone waren teilweise kontrahiert und zeigten auf Querschnitten unregelmäßige Form. Gelegentlich fand sich eine Vergrößerung der axonalen Mitochondrien und Zerstörung ihrer inneren Parallelstrukturen. Als Hauptfaktoren in der Pathogenese der Hirnveränderungen nach experimenteller CO_Vergiftung werden zerebrale Anoxie infolge CO-Hämoglobinbildung, cytotoxische Wirkung von CO auf das Hirngewebe und eine Störung der Blutzirkulation angenommen.
    Notes: Summary In experimental carbon monoxide poisoning, more marked and widespread pathological changes in the brain were seen in the white matter as compared with the gray matter. No essential difference, in the appearance of cerebral alterations, was detected between acute and chronic carbon monoxide poisoning. It was confirmed that the changes resulting from chronic poisoning showed a higher degree of severity as compared with those resulting from acute poisoning. Nerve cells with slightly dilated or fragmented endoplasmic reticulum and Golgi complex in the gray matter were found infrequently. However, most mitochondria did not show any kind of abnormality in such cells. A remarkable dilatation of the endoplasmic reticulum of oligodendrocytes was sometimes seen in chronic cases. Capillaries remained intact and astrocytic perivascular foot-plates were not swollen. In the white matter, the normal constituents of the myelin sheath were partially lost and the myelin lamellae showed moderate exfoliation, or a change into homogenous or structureless substance. Myelinated axons were partially contracted and showed an irregularity in shape in transverse sections. Enlargement of occasional axonal mitochondria and the destruction of their internal parallel structures were observed. Cerebral anoxia caused by carboxyhaemoglobin, together with the cytotoxic action of carbon monoxide on the brain tissue and the disturbance of blood circulation in the brain are suggested as the chief factors in the pathogenesis of the cerebral alterations induced by experimental carbon monoxide poisoning.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00686348
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  • 24
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    Electronic Resource
    Electron microscopic alterations in the vicinity of epileptogenic cobalt-gelatine necrosis in the cerebral cortex of the rat (1969)
    Springer
    Acta neuropathologica 14 (1969), S. 201-214 
    Details
    ISSN: 1432-0533
    Keywords: Electron Microscopy ; Epilepsy, Experimental ; Focus ; Cobalt Gelatine ; Plasmatic Infiltration ; Brain Edema
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Bei 25 erwachsenen Ratten wurde ein epileptogener Kobaltgelatineherd in einer Standardregion der Hirnrinde erzeugt. Die Tiere wurden am 1., 3., 5., 8., 14. 21. und 28. Tag nach der Operation getötet. Das Gebiet des epileptogenen Herdes wurde elektronenmikroskopisch untersucht. Die frühesten Veränderungen bestanden in ödem. Später traten Veränderungen der Astrocytenfortsätze auf, die sich an die Basalmembranen der Capillaren anlegten; die Intercellularräume erweiterten sich, füllten sich mit elektronenoptisch dichten Massen und entwickelten ein “plasmatisches Infiltrat”. Allmählich breitete sich dieses Exsudat weiter um die Capillaren aus; es trat an Orten auf, wo die Astrocytenfortsätze und die Dendriten schwer geschädigt waren und erfüllte die weiteren Intercellularräume des Neuropils. Die Nervenzellen zeigten eine auffallende Widerstandsfähigkeit gegenüber diesem pathologischen Prozeß. Manchmal waren sie umschlossen von Exsudatmassen und zum Teil oder ganz der zarten neuralen und Astrocytenfortsätze beraubt, von denen sie normalerweise umgeben sind. Unsere Befunde werden als das ultrastrukturelle Korrelat der Schols'schen “plasmatischen Infiltration” interpretiert.
    Notes: Summary In 25 adult rats an epileptogenic cobalt-gelatine focus was produced in a standard region of the cerebral cortex. The animals were killed successively on the 1st, 3rd, 5th, 8th, 14th, 21st and 28th day after operation and the region of the epileptogenic focus was examined electron microscopically. The earliest changes were those of oedema. Later there occurred disturbances of the astrocytic processes which cling to the basement membranes of the capillaries; the intercellular spaces widened and filled with rather E. M. dense masses and a “plasmatic infiltrate” developed. Gradually this exudative substance spread even further from the capillaries. It appeared in places where the astrocytic processes were severely demaged and the dendrites were partly dissipated and it filled out the wide intercellular spaces of the neuropil. The nerve cells showed a striking resistance toward this pathological process. Sometimes they floated in the exudative masses but were partly or totally deprived of the fine neural and astrocytic processes which normally surround them. Our findings are interpreted as the ultrastructural correlate of Scholz's “plasmatic infiltration”.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00685300
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  • 25
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    Electronic Resource
    Étude d'une nouvelle observation de «Nemaline Myopathy» (1969)
    Springer
    Acta neuropathologica 13 (1969), S. 250-266 
    Details
    ISSN: 1432-0533
    Keywords: Electron Microscopy ; Congenital Myopathy ; Nemaline Myopathy ; Z-band Structure ; Sarcoplasmic Masses
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Résumé L'étude ultrastructurale d'une observation de «Nemaline-Myopathy» a été centrée sur les rapports structurels entre bâtonnets et stries Z normales dans différentes conditions de fixation. L'aspect des stries Z, en section transversale, varie en effet avec la fixation: réseau de ponts «obliques» (par rapport à l'alignement quadratique régulier des terminaisons des filaments fins) après fixation osmiée, réseau de ponts «transversaux», isolé ou superposé au premier réseau, après fixation aldéhydique initiale. L'aspect des bâtonnets en section transversale varie également avec la fixation; la disposition quadratique des filaments constitutifs des bâtonnets est visible après l'une et l'autre fixation, mais un réseau quadratique régulier, de 75 Å de côté, n'est clairement apparent qu'après fixation aldéhydique. Le point important est que ce dernier aspect diffère complètement du réseau «oblique» des stries Z normales, et peut être rapproché au contraire du réseau «transversal» de la strie Z obtenu après fixation aldéhydique; l'hypothèse est ainsi soulevée de la constitution des ponts dans les bâtonnets aux dépens d'un seul des deux constituants morphologiques de la strie Z. La topographie des bâtonnets a été également étudiée: tantôt périphérique, au sein de masses sarcoplasmiques latérales, tantôt centrale, au sein de petits foyers de désintégration myofibrillaire. La structure des plaques motrices visibles dans les préparations était normale. La spécificité de la lésion élémentaire et l'autonomie de ce type de myopathie sont ensuite discutées.
    Notes: Summary Ultrastructural study of an observation of “Nemaline Myopathy” has been focused on structural relationships between rods and normal Z-bands in different conditions of fixation. The Z-band structure studied in transversal section, varies with the fixation: network of “oblique” (relative to the regular quadratic pattern of the thin filament endings) bridges after osmic fixation; network of “transversal” bridges, alone or associated to the first network, after aldehydic fixation. The rod structure, in transversal section, is also varying with the fixation: a quadratic disposal of the filamentary content is visible after both fixations, but a regular quadratic network, 75–85 Å side, is clearly appearent only after aldehydic fixation. This last network differs from the “oblique” network of the normal Z-band; it can be compared to the “transversal” one obtained in the Z-band after aldehydic fixation. The hypothesis that bridges in the rods are formed only by one of the two normal morphological components of the Z-band, is raised. Topography of the rods in muscle fibers has been studied: either in peripheral sarcoplasmic masses, or in small central-located foci of myofibrillar disintegration. The end-plates structure was normal. Specificity of the elementary lesion, and autonomy of that type of myopathy is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00690645
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  • 26
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    Electronic Resource
    Sodium demonstration in rat cerebrum following perfusion with hydroxyadipaldehyde-antimonate (1969)
    Springer
    Acta neuropathologica 12 (1969), S. 173-182 
    Details
    ISSN: 1432-0533
    Keywords: Sodium Deposits ; Electron Microscopy ; Perfusion with Hydroxyadipaldehyde-Antimonate ; Extracellular Space
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Eine Modifikation der Methode zur ultrastrukturellen Darstellung von Natrium wird beschrieben, die eine Perfusion mit leicht alkalischer Mischung von Hydroxydipaldehyd, Phosphatpuffer und Kalium-Pyro-Antimonat verwendet. Im normalen Gehirn werden keine Natrium-Deposite angetroffen. Das spricht dafür, daß im Gehirn keine intraoder extracelluläre Konzentration über 150 mM vorliegt. Bei Vorliegen hoher NaCl-Konzentration sieht man ein großes, dichtes Präcipitat, das nach direkter Injektion in den extracellulären Räumen, Neuronen und Astrocyten lokalisiert werden kann. Im Mark der vergifteten Ratten findet sich ähnlich wie nach direkter Natrium-Injektion ein diskretes, gleichfalls als Natrium aufgefaßtes Präcipitat an der Oberfläche von Oligodendrogliazellen, aber nicht innerhalb dieser Zellen. Ein elektronendichtes Deposit unklarer Herkunft findet sich in den Myelinspalten, die nach akuter TET-Vergiftung auftreten. Der Wechsel in Charakter und Lokalisation dieser elektronendichten Partikel mahnt zur Vorsicht in ihrer Interpretation bei histochemischen Untersuchungsmethoden.
    Notes: Summary A modification of the technique for the ultrastructural demonstration of sodium is described which involves a perfusion with a slightly alkaline mixture of hydroxyadipaldehyde, phosphate buffer and potassium pyroantimonate. No sodium deposits are found in normal brain indicating that no intra or extracellular concentration exceeding 150 mM is present in the brain. In the presence of high concentration of NaCl, a large dense precipitate can be seen which after a direct injection can be localized to extracellular spaces, neurons and astrocytes. In the white matter of tin poisoned rats, as well as after the direct injection of sodium, a discrete precipitate, also believed to represent sodium, is noted at the surface of oligodendrocytes but not within these cells. An electron dense deposit of dubious origin is present in the myelin clefts which occur as a result of acute triethyltin intoxication. The variations in the character and localization of these electron dense particles emphasize the caution which is required in their interpretation following histochemical procedures.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00692504
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  • 27
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    Electronic Resource
    Züchtung und Vermehrung von Herpes simplex-Virus in Zellkulturen vom Nervensystem (1969)
    Springer
    Acta neuropathologica 12 (1969), S. 276-299 
    Details
    ISSN: 1432-0533
    Keywords: Herpes simplex Virus ; CNS Tissue Culture ; Multiplication in vitro ; Cytopathic Alterations ; Electron Microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Die häufig diskutierte, (Mit)beteiligung von Herpes simplex-Virus (HSV) bei bestimmten Alterationen im ZNS sowie der hohe Neutropismus des HSV führte zur Frage, inwieweit sich HSV auch in in vitro gezüchteten, spezifischen Zellen vom Nerven-system vermehren und züchten läßt, und ob sich hierbei licht-und elektronenmikroskopisch charakteristische Alterationen nachweisen lassen. Es zeigte sich, daß sich das HSV in allen verschiedenen angezüchteten spezifischen Zellen (von Plexus chorioideus des Rhesusaffen, Kaninchenspinalganglien, menschlichen Oligodendrogliomen, Meningeomen sowie fibrillären und protoplasmatischen Astrocytomen) kultivieren und vermehren läßt. Hierbei kommt es in den spezifischen Zellen nicht nur lichtmikroskopisch zu charakteristischen cytopathischen Veränderungen, sondern auch biologisch zu einer signifikanten Vermehrung des Virus in diesen Zellen. Auch elektronenmikroskopisch lassen sich viele der charakteristischen Bildungs-, Reifungs- und Ausschleusungsstadien des Virus in den Kernen und im Cytoplasma der Zellen beobachten. Die Besonderheiten der elektronenmikroskopisch erhobenen ultrastrukturellen Befunde werden ausführlich beschrieben, und die sich daraus abzuleitenden Möglichkeiten werden im Hinblick auf die biologischen Eigenheiten des HSV bei in vivo-Infektionen, aber auch im Hinblick auf die Deutung lichtmikroskopisch histochemischer und immunhistologischer Befunde nach in vivo-Infektionen mit diesem Virus ausführlich diskutiert.
    Notes: Summary The participation of herpes simplex virus (HSV) in certain alterations of the CNS has been partially questioned and frequently discussed. Therefore, the question arose whether HSV can also be cultivated and multiplied in vitro in CNS specific cells, and if so, whether characteristic alterations and structures can be observed by means of light and electron-microscopic examinations. Our data demonstrate that HSV multiplies in all different specific cells originating from plexus chorioideus of rhesus monkeys, from spinal ganglia of rabbit, from human oligodendroglioma, meningeoma as well as from fibrillar and protoplasmatic astrocytoma. Characteristic cytopathic alterations of these specific cells and significant multiplication of the virus in these cells are to be found. Many of the characteristic forms of formation, maturation and release of HSV can be seen by electronmicroscopic examinations. The particular ultrastructural data observed by electron microscopy are described in detail and the resulting possibilities are broadly discussed not only with regard to the biologic particularity of HSV in in vivo infection but also in view of the interpretations, deriving from histochemical and immunohistological data obtained by light microscopy after in vivo infection.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00687650
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  • 28
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    Electronic Resource
    An electron microscopic study of meningiomas (1969)
    Springer
    Acta neuropathologica 13 (1969), S. 301-323 
    Details
    ISSN: 1432-0533
    Keywords: Meningiomas ; Electron Microscopy ; Endotheliomatous, Fibromatous Type ; Light Cells ; Dark Cells ; Histogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung 17 chirurgisch entfernte Meningiome vom endotheliomatösen und 5 vom fibromatösen Typ wurden elektronenmikroskopisch untersucht. Unterschiede im Entwicklungsgrad der cytoplasmatischen Fortsätze und der Beziehungen zwischen den Plasmamembranen der Tumorzellen wurden in den endotheliomatösen Meningeomen beobachtet. In 4 fibromatösen Tumoren sind die zelligen Areale von bindegewebigen Räumen umgeben. Auch wenn die meisten Tumorzellen helle Zellen sind und sich nicht wesentlich von den Tumorzellen der endotheliomatösen Meningiome unterscheiden, ist die histologische Anordnung der fibromatösen klar von derjenigen der endotheliomatösen Meningeome zu trennen. Ausschlaggebend dafür ist die starke Entwicklung des Bindegewebes. Die Dunkelzellen lassen sich in zwei Typen gliedern: Typ I wird in vier Tumoren mit stark ausgebildetem Bindegewebe angetroffen. Die Analyse der inneren Zellstruktur sowie das Vorliegen von Übergängen zwischen hellen und dunklen Zellen machen es wahrscheinlich, daß diese nur verschiedene Typen einer einzigen Ursprungszelle darstellen. Die Bedeutung dieser verschiedenen Erscheinungsformen der Meningiomzellen wird diskutiert. Die Ultrastruktur der Tumorzellen ähnelt derjenigen der normalen arachnoidalen Zellen. Rückschlüsse auf die blastodermale Herkunft der normalen und tumoralen meninigalen Zellen auf Grund ihrer ultrastrukturellen Merkmale erscheinen verfrüht.
    Notes: Summary Seventeen surgically removed meningiomas of the endotheliomatous and five of the fibromatous type were investigated with an electron microscope. Differences of the development of cytoplasmic processes and the relations between plasma membranes of blastomatous cells were observed in endotheliomatous meningiomas. In four of the fibromatous tumors the cell groups were surrounded by spaces of connective tissue. Although most of the tumor cells were light cells and are not essentially different from the tumor cells of the endotheliomatous meningiomas, the histological structure of fibromatous meningiomas is clearly distinguished from the endotheliomatous type, because of the greater amount of connective tissue. The dark cells may be divided into two types: the first was found in those four tumors, where the connective tissue is well developed, while the second one only occurred in one of the tumors. The analysis of the inner cell structure as well as the presence of interstages between dark and light cells makes it probable that dark and light cells are different types of one original cell. The cause of this different appearance of the menigioma cells is discussed. The fine structure of the tumor cells showed a great similarity with that of normal arachnoidal cells. Conclusions on the blastodermic origin of normal and blastomatous meningial cells on the basis of ultrastructural characteristics, however, seem to be premature.
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    URL: http://dx.doi.org/10.1007/BF00686120
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  • 29
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    Electronic Resource
    Étude ultrastructurale de quatre cas de leuco-encéphalite sclérosante subaiguë (1969)
    Springer
    Acta neuropathologica 14 (1969), S. 1-13 
    Details
    ISSN: 1432-0533
    Keywords: Subacute Sclerosing Leucoencephalitis ; Electron Microscopy ; Tubular Inclusions ; Inclusion Bodies (Cristalline Rods, Fibrillar Bundles, Nuclear Bodies, Osmiophilic Particles)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Résumé Les auteurs rapportent 4 observations caractéristiques de LESS: L'analyse ultra-structurale montre: 10 — des inclusions tubulaires intra-nucléaires neuronales ou gliales et intra-axonales de morphologie superposable à celle des nucléocapsides des myxo-virus, de la rougeole en particulier. 20 — des formations non spécifiques: corps nucléaires, faisceaux cristallins et fibrillaires traduisant un trouble métabolique nucléaire, particules osmiophiles liées à une hyperactivité cellulaire. Les inclusions tubulaires virales sont présentes quelque soit la durée de la maladie (3 mois à 5 ans) indiquant l'absence d'autostérilisation virale dans le S.N.C. La LESS apparaît liée au virus rougeoleux mais le mécanisme indirect immunitaire ou métabolique de cette action est inconnu.
    Notes: Summary Four cases of S.S.L.E are reported. The electron microscopy findings show: 1. Tubulary inclusion bodies: They appear in three cases out of four, either in neuronal and glial nucleus or in axis cylinders. Their morphological features are similar to the nucleocapsides of myxoviruses and particularly measles-virus. 2. Other different types of nonspecific inclusions: nuclear bodies, cristalline-like rods and fibrillar bundles which may be considered as the result of a nuclear metabolic disorder, osmiophilic particles which may be seen as the result of a cellular intracytoplasmic hyperactivity. The tubules are found whatever the duration of the disease (3 months up to 5 years) may be. This might indicate that there is no autosterilization of the virus in the C.N.S. during the course of S.S.L.E. According to the most recent papers, such a disease might be interpreted as an infection indirectly induced by measles-virus with an unknown immunologic or metabolic mechanism.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00687698
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  • 30
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    Electronic Resource
    The normal sural nerve in man (1969)
    Springer
    Acta neuropathologica 13 (1969), S. 197-216 
    Details
    ISSN: 1432-0533
    Keywords: Peripheral Nerves ; Axons ; Myelin Sheaths ; Schwann Cells ; Electron Microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Eine kombinierte licht- und elektronenmikroskopische Untersuchung am normalen N. suralis von 7 Menschen im Alter von 15–59 Jahren wurde vorgenommen. Qualitative und quantitative Beobachtungen an Schwann-Zellen und Fibroblasten, markhaltigen und marklosen Fasern wurden an isolierten Faszikeln durchgeführt. Schwannzellen überwiegen gegenüber Fibroblasten im Verhältnis von etwa 9:1. Die meisten Schwannzellen (etwa 80%) liegen an marklosen Fasern. Die Faktoren, welche die Dichte dieser Zellen pro Querschnittsareal beeinflußen, werden diskutiert. Einige ultrastrukturelle Befunde an bemarkten Fasern werden beschrieben und ihre Zahl pro mm2 sowie die Häufigkeitsverteilung ihrer Dicke wird angegeben. Eine indirekte Methode zur Bestimmung der mittleren Internodienlänge für jede der Markfasergrößenpopulationen an Querschnitten von Faszikeln normaler Nerven durch Bestimmung des Verhältnisses der markhaltigen Fasersegmente zu ihrer Kernzahl wird vorgeschlagen. Die Ultrastruktur der marklosen Nervenfaern wird beschrieben und die Identifizierung dieser Axone mit extremen Durchmessern diskutiert. Ihre Dichte und Größenfrequenzhistogramme sind die ersten, die am Menschen durch systematische elektronenoptische Untersuchungen veröffentlicht werden. Das mittlere Verhältnis von marklosen zu bemarkten Fasern ist etwa 3,7:1 und schwankt in den Faszikeln der Einzelindividuen.
    Notes: Summary A combined light and electron microscope study of the normal sural nerve in 7 people aged 15–59 years is reported. Qualitative and quantitative studies of the Schwann cells and fibroblasts, myelinated and unmyelinated fibres are made in isolated fascicles. Schwann cells predominate over fibroblasts in the ratio of about 9-1. Most Schwann cells, almost 80%, are attached to unmyelinated fibres. Factors influencing the densities of these cells per cross sectional area are discussed. Some ultrastructural features of the myelinated fibres are described and their numbers per sq.mm and frequency distribution of their sizes are produced. An indirect method is proposed for assessing the mean internodal length for earch of the myelinated fibre size populations in cross sections of fascicles of normal nerves by estimating the proportion of myelinated segments cut through their nucleus. The ultrastructure of unmyelinated fibres is described and the identification of axons of extreme diameter is discussed. Their densities and size frequency histograms are the first to be reported in man by systematic electron microscope studies. The average ratio of unmyelinated to myelinated fibre density is about 3.7:1 though it varies in the fascicles of the different individuals. The implications of axonal diameter in the presence of myelin are commented on.
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    URL: http://dx.doi.org/10.1007/BF00690642
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  • 31
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    Electronic Resource
    Light and electron microscopic study of the muscles in hypokalemic periodic paralysis (1969)
    Springer
    Acta neuropathologica 12 (1969), S. 329-338 
    Details
    ISSN: 1432-0533
    Keywords: Hypokalemic Periodic Paralysis ; Electron Microscopy ; Myopathy ; Sarcoplasmic Reticulum ; Glycogen, Muscular
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Licht- und elektronenoptische Untersuchungen wurden an Muskelbiopsien von 4 Fällen hypokaliämischer periodischer Paralyse durchgeführt. Die frühesten Veränderungen betreffen nach Meinung der Verfasser das sarkoplasmatische Reticulum: Vacuolisierung des Längssystems und vermutlich Multiplikation des Quersystems. Es wird vermutet, daß die Vacuolenbildung die Folge einer Fusion der Vesikeln des Quersystems darstellt. Destruktion von Myofibrillen und Vergrößerung der, interfibrillären Räume treten erst in späteren Phasen des Krankheitsprozesses auf. Ausdehnung und Intensität dieser Veränderungen bedingen das typische histologische Bild myogener Läsionen bei Fällen m klinisch fixierten Symptomen der Myopathie.
    Notes: Summary Light and electron-microscopic studies were performed on the muscles taken from 4 cases with hypokalemic periodic paralysis. The authors conclude that the earliest changes concern the sarcoplasmic reticulum: vacuolisation of the longitudinal system and probably, multiplication of the transverse system. It is suggested, that vacuoles are formed as a result of fusion of the vesicles of the transverse system. Destruction of myofibrilles and enlargement of the interfibrillar spaces appear at a later period of the disease process. Extensive and intensive changes of that kind present a typical appearance of myogenic changes in histological study observed in the cases with clinically fixed symptomes of myopathy.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00809129
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    The inhibition of calcium hydroxyapatite crystal growth by polyphosphonates and polyphosphates (1969)
    Springer
    Calcified tissue international 3 (1969), S. 151-162 
    Details
    ISSN: 1432-0827
    Keywords: Calcification ; Physiologic ; Phosphonic Acids ; Phosphates ; Crystallization ; Electron Microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Description / Table of Contents: Résumé On a étudié la formation de l'hydroxyapatite de calcium cristallin à partir de solutions d'ions de calcium et de phosphate et l'inhibition de la croissance de cristaux de l'hydroxyapatite de calcium au moyen de polyphosphonates et de polyphosphates. Les polyphosphonates, éthane hydroxy-1-diphosphonate-1,1 de disodium et dichlorométhanediphosphonate de disodium, sont inhibiteurs efficaces contre la croissance de cristaux de l'hydroxyapatite de calcium. Les polyphosphates sont aussi inhibiteurs efficaces contre la croissance de cristaux de l'hydroxyapatite de calcium tant que le niveau exigé de polyphosphate intact est présent dans le système. Cependant, à cause de leur instabilité hydrolytique, qui est soulignée par une température élevée, valeur de pH basse, et certaines enzymes, la concentration du polyphosphate diminue avec le tempsin vitro, et son activité comme inhibiteur est perdue. Au contraire aux polyphosphates, les polyphosphonates sont hydrolytiquement stables. Les polyphosphonates sont chimiosorbés sur la surface des microcristallites de l'hydroxyapatite de calcium, ainsi empêchant l'occurrence d'autre croissance de cristaux semblable à l'action d'autres poisons connus de croissance de cristaux. On propose l'extension de cette action sur la formation de l'apatite et cette stabilité des polyphosphonates aux applications médicales et dentaires concernant le metabolisme pathologique de calcium et de phosphate.
    Abstract: Zusammenfassung Die Bildung des kristallinen Calciumhydroxyapatit aus Lösungen, welche Calcium- und Phosphationen enthalten, und die Hemmung der Bildung von kristallinen Calciumhydroxyapatit durch Polyphosphonate und Polyphosphate wurden untersucht. Polyphosphonate, Dinatriumäthan-1-hydroxyl-1,1-diphosphonat und Dinatriumdichloromethandiphosphonate verhindern das Kristallwachstum des Calciumhydroxyapatits. Die Polyphosphate verhindern ebenfalls das Kristallwachstum des Calciumhydroxyapatits, solange die notwendige Konzentration des nicht hydrolysierten Polyphosphats vorhanden ist. Wegen ihrer hydrolytischen Unbeständigkeit, die durch hohe Temperatur, niedrige pH und bestimmte Enzyme erhöht wird, vermindert sich jedoch die Konzentration des Polyphosphats allmählichin vitro, und ihre Hemmungsaktivität geht verloren. Im Gegensatz zu den Polyphosphaten sind die Polyphosphonate hydrolytisch beständig. Die Polyphosphonate werden an der Oberfläche der Mikrokristallite des Calciumhydroxyapatits chemisorbiert und verhindern, wie andere bekannte Kristallwachstumsgifte, auf diese Weise weiteres Kristallwachstum. Die Beständigkeit der Polyphosphonate und ihre Chemisorption an dem Apatit empfehlen ihren Gebrauch in der ärztlichen und zahnärztlichen Praxis, soweit sie den pathologischen Calcium- und Phosphatstoffwechsel betreffen.
    Notes: Abstract The formation of crystalline calcium hydroxyapatite from solutions of calcium and phosphate ions and the inhibition of calcium hydroxyapatite crystal growth by polyphosphonates and polyphosphates have been studied. The polyphosphonates, disodium ethane-1-hydroxy-1,1-diphosphonate and disodium dichloromethane diphosphonate, are effective inhibitors of calcium hydroxyapatite crystal growth. The polyphosphates are also effective inhibitors of calcium hydroxyapatite crystal growth as long as the required level of intact polyphosphate is present in the system. However, because of their hydrolytic instability, which is enhanced by high temperature, low pH, and certain enzymes, the concentration of the polyphosphate decreases with timein vitro, and its activity as an inhibitor is lost. In contrast to the polyphosphates, the polyphosphonates are hydrolytically stable. The polyphosphonates are chemisorbed on the surface of the microcrystallites of calcium hydroxyapatite and, in the manner of other known crystal growth poisons, thus prevent further crystal growth. The stability of the polyphosphonates and their chemisorption on apatite suggest their use in medical and dental applications involving pathological calcium and phosphate metabolism.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02058658
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    Relevance of sodium transport pool measurements in toad bladder tissue for the elucidation of the mechanism whereby hormones stimulate active sodium transport (1969)
    Springer
    Pflügers Archiv 313 (1969), S. 197-221 
    Details
    ISSN: 1432-2013
    Keywords: Toad Bladder ; Sodium Transport Pool ; Aldosterone ; Insulin ; Vasopressin ; Krötenblase ; Natrium-Transport-Pool ; Aldosteron ; Insulin ; Vasopressin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The size of toad bladder sodium transport pool, defined as that amount of sodium of apical origin and recovered in tissue at equilibrium, was compared with sodium transport rate, derived from short-circuit current read immediately before tissue analysis. Provided certain precautions be taken, the relationship between both variables can be described by a curve starting at the intersect ofX (pool, in μEq) andY (transport, in μEq/hr) axes, with a tendency forX to increase faster thanY. Assuming sodium transport pool forms one compartment, its calculated half-life averages 2–3 min. sodium transport pool measurements are thought to shed light on mechanism of sodium transport by toad bladder because pool size was large with respect to transport rate when tissue was exposed to several inhibitors of sodium transport. Conversely, upon stimulation of activity of (substrate — depleted) preparations by glucose, a relative reduction of pool size was observed. Aldosterone, vasopressin (and adenosine 3′,5′-phosphate) increased sodium pool size and transport rate, proportionately; insulin stimulated sodium transport more than it increased pool size. Thus, insulin presumably exerts its effect at the sodium “pump” while such a site of action need not be postulated for aldosterone and vasopressin: these 2 hormones would instead induce, permeability changes faciliting sodium movement at the apical border of toad bladder epithelial cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00586744
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    Electronic Resource
    Procedure for double antibody immunoassay of insulin (1969)
    Springer
    Acta diabetologica 6 (1969), S. 371-388 
    Details
    ISSN: 1432-5233
    Keywords: Insulin ; Insulin antibodies ; Insulin assay ; Laboratory technique
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01548060
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  • 35
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    Variaciones diurnas de la eliminación de insulina en orina (1969)
    Springer
    Acta diabetologica 6 (1969), S. 197-205 
    Details
    ISSN: 1432-5233
    Keywords: Diurnal variations ; Food intake ; Glucose load ; Insulin ; Tolbutamide test
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Resume On a étudié l'effet de la lumière et de la période d'ingestion des aliments sur l'élimination d'insuline par les urines. On peut démontrer l'existence d'un rythme circadien et la présence de deux facteurs de régulation pour cette élimination. On présente quelques hypothèses concernant ces phénomènes.
    Abstract: Resumen Se estudió el efecto que sobre la eliminación de insulina urinaria ejercen los períodos de luzoscuridad y el horario de administración del alimento. Se demuestra la existencia de un ritmo circadiano de eliminación de dicha hormona, describiéndose dos reguladores del mismo. Se hacen especulaciones fisiopatológicas acerca de su significado.
    Notes: Riassunto E' stato studiato l'effetto della luce e del periodo di ingestione del cibo sull'eliminazione di insulina nelle urine. Si può dimostrare l'esistenza di un ritmo circadiano e la presenza di due fattori di regolazione per questa eliminazione. Vengono presentate alcune ipotesi concernenti questi fenomeni.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01548049
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    Proprietà insulino-stimolanti della glibenclamide (HB 419) in soggetti normali. Studi comparativi con la tolbutamide (1969)
    Springer
    Acta diabetologica 6 (1969), S. 283-298 
    Details
    ISSN: 1432-5233
    Keywords: Glybenclamide ; Insulin ; Insulin secretion ; Sulfonylureas ; Tolbutamide
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Resume Les effets métaboliques de la glybenclamide et de la tolbutamide ont été étudiés chez deux groupes de sujets normaux traités avec des doses comparables des deux sulphanylurées. En rapport aussi à la dose de 1:400, la glybenclamide a démontré une activité insuline-sécrétive bien majeure de celle de la tolbutamide. La réponse insulinique a été suivie par une rapide et prolongée diminution des NEFA sériques et par un moins persistant effet hypoglycémique. Les effets métaboliques de l'HB 419 sont encore évidents 10–12 heures après l'administration du médicament, tandis que l'activité de la tolbutamide est terminée géneralement entre 4–6 heures. Quand l'administration des médicaments a été pratiquée en concomitance avec une charge veineuse de glucose, le coéfficient de Conard était élevé d'une façon significative par toutes les deux sulphanylurées.
    Abstract: Resumen Los efectos metabólicos de la glibenclamida y de la tolbutamida han sido investigados en dos grupos de sujetos normales tratados con dosis comparables de las dos sulfanilúreas. Inclusive en una relación de dosificación de 1:400, la glibenclamida ha demostrado una actividad insulinosecretora mucho mayor de la tolbutamida. La respuesta insulínica ha sido seguida de una disminución pronta y prolongada de los NEFA séricos y de un efecto hipoglucemiante menos persistente. Los efectos metabólicos del HB 419 son aún evidentes a las 10–12 horas después de la administración del medicamento, mientras la actividad de la tolbutamida es anulada por lo general dentro de 4–6 horas. Cuando la suministración de los medicamentos ha sido practicada en coincidencia con una carga venosa de glucosa, el coeficiente de Conard apareció significativamente elevado por ambas sulfanilúreas.
    Notes: Riassunto Gli effetti metabolici della glibenclamide e della tolbutamide sono stati indagati in 2 gruppi di soggetti normali trattati con dosi comparabili delle due sulfaniluree. Anche in un rapporto di dosaggio di 1:400, la glibenclamide ha mostrato un'attività insulino-secretiva assai maggiore di quella della tolbutamide. La risposta insulinica è stata seguita da una pronta e prolungata diminuzione dei NEFA serici e da un meno persistente effetto ipoglicemico. Gli effetti metabolici del-l'HB 419 sono ancora evidenti 10–12 h dopo la somministrazione del farmaco mentre l'attività della tolbutamide si annulla generalmente entro 4–6 h. Quando la somministrazione dei farmaci è stata praticata in coincidenza con un carico venoso di glucosio, il coefficiente di Conard è apparso significativamente innalzato da entrambe le sulfaniluree.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01548054
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    Studies on the transport of insulin antibodies across the placenta to the fetus and their effect on the fetal pancreatic islet system (1969)
    Springer
    Acta diabetologica 6 (1969), S. 573-584 
    Details
    ISSN: 1432-5233
    Keywords: Insulin ; Insulin antibodies ; Islets of Langerhans ; Placenta ; Transport
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Resume Ce travail est la continuation des communications préliminaires sur la résistance insulinique des nouveaux-nés de mères insuline-résistantes. Les recherches ont été conduites sur cobayes et sur sujets humains. Les AA. ont démontré que les anticorps anti-insuline passent dans la circulation fœtale et provoquent l'apparition d'une insuline-résistance passive qui va disparaître dans les premières semaines de la vie. Les anticorps contre l'insuline bovine lient en partie l'insuline endogène des cobayes, ce qui est mis en evidence par l'hyperglycémie élevée des cobayes jeunes nées de mères insuline-résistantes. Les anticorps anti-insuline n'ont aucun effet important sur la structure du pancréas des cobayes dans la période périnatale.
    Abstract: Resumen Este trabajo representa la continuación de las comunicaciones preliminares sobre la resistencia insulínica que presentan las personas nacidas de madres insulino-resistentes. Las investigaciones han sido efectuadas en cobayas y hombres. Se ha demostrado que los anticuerpos anti-insulina pasan al círculo fetal, provocando la aparición de insulino-resistencia pasiva, que desaparece durante las primeras semanas de vida. Los anticuerpos contra la insulina bovina ligan en parte la insulina endógena de cobayas, como lo demuestra la hiperglucemia elevada de las cobayas jóvenes nacidas de madres insulino-resistentes. Los anticuerpos anti-insulina no ejercen ningún efecto apreciable sobre la estructura del páncreas de las cobayas durante el período peri-natal.
    Notes: Riassunto Questo lavoro rappresenta la continuazione delle comunicazioni preliminari sulla resistenza insulinica dei nati di madri insulino-resistenti. Le ricerche sono state condotte su cavie e su uomini. E' stato dimostrato che gli anticorpi anti-insulina passano nel circolo fetale inducendovi la comparsa di una insulino-resistenza passiva che scompare nelle prime settimane di vita. Gli anticorpi contro l'insulina bovina legano in parte l'insulina endogena di cavie, come è evidenziato dall'elevata iperglicemia delle cavie giovani nate da madri insulino-resistenti. Gli anticorpi anti-insulina non esercitano alcun effetto di rilievo sulla struttura del pancreas delle cavie nel periodo perinatale.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01548073
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    Electronic Resource
    Insulin allergy. Clinical and immunological effects of specific desensitization (1969)
    Springer
    Acta diabetologica 6 (1969), S. 796-808 
    Details
    ISSN: 1432-5233
    Keywords: Desensitization ; Immunoglobulins ; Insensitivity ; Insulin ; Insulin allergy ; Insulin antibodies ; Macroglobulins ; Porcine insulin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Resume Cinq patients atteints d'allergie à l'insuline ont été désensibilisés par de l'insuline de porc selon la méthode d'Urbach et Gotlieb. Le diagnostic d'allergie à l'insuline a été posé sur la base des manifestations cliniques, de la positivité des réactions cutanées et, dans 2 cas, des réactions de Prausnitz-Küstner (P.K.). On a obtenu dans 3 cas une désensibilisation. La présence d'anticorps anti-insuline a été révélée par la méthode immunoautoradiographique en employant de l'insuline131J. Avant la désensibilisation la présence d'anticorps IgG a été enregistrée chez 3 patients tandis que chez 2 on a démontré également des anticorps IgA. Une réaction de P.K. positive a été observée chez des patients dont les sérums contenaient des anticorps IgA. Pendant la désensibilisation on a observé une apparition transitoire de macroglobulines capables de lier l'insuline131J. Après la désensibilisation on a constaté la persistance de la positivité aux réactions cutanées immédiates à l'insuline de porc et de boeuf. En même temps les réactions retardées et la réaction de P.K. à l'insuline de porc étaient interverties, bien que les mêmes réactions à l'insuline bovine restaient positives.
    Abstract: Resumen Cinco pacientes que padecían de alergia a la insulina han sido desensibilizados con insulina porcina según el método de Urbach y Gotlieb. El diagnóstico de alergia a la insulina ha sido puesto teniendo en cuenta las manifestaciones clínicas, el positivismo de las reacciones cutáneas, y, en 2 casos, las reacciones de Prausnitz-Küstner (P.K.). En 3 casos se ha obtenido desensibilización. La presencia de anticuerpos anti-insulina ha sido puesta en evidencia con el método inmunoautoradiográfico, empleando insulina131J. Antes de la desensibilización, la presencia de anticuerpos IgG ha sido hallada en 3 pacientes, mientras que en 2 han sido demostrados también anticuerpos IgA. Una reacción de P.K. positiva ha sido observada en pacientes cuyos sueros contenían anticuerpos IgA. Durante la desensibilización se ha observado aparición transitoria de macroglobulinas capaces de ligar la insulina131J. Al cabo de la desensibilización se ha notado la persistencia del positivismo a las respuestas cutáneas inmediatas a la insulina porcina y bovina. Al mismo tiempo, las respuestas retardadas y la reacción de P.K. a la insulina porcina cstaban invertidas, no obstante que las mismas reacciones a la insulina bovina hubieran quedado positivas.
    Notes: Riassunto Cinque pazienti con allergia all'insulina sono stati desensibilizzati con insulina suina secondo il metodo di Urbach e Gotlieb. La diagnosi di allergia all'insulina è stata posta sulla base delle manifestazioni cliniche, della positività delle reazioni cutanee e, in 2 casi, delle reazioni di Prausnitz-Küstner (P.K.). In 3 casi si è ottenuta una desensibilizzazione. La presenza di anticorpi anti-insulina è stata rivelata con il metodo immunoautoradiografico impiegando insulina131J. Prima della desensibilizzazione la presenza di anticorpi IgG è stata riscontrata in 3 pazienti mentre in 2 sono stati dimostrati anche anticorpi IgA. Una reazione di P.K. positiva è stata osservata in pazienti i cui sieri contenevano anticorpi IgA. Durante la desensibilizzazione si è osservata una comparsa transitoria di macroglobuline capaci di legare l'insulina131J. Dopo la desensibilizzazione si è riscontrata la persistenza della positività delle risposte cutanee immediate all'insulina suina e bovina. Al tempo stesso le risposte ritardate e la reazione di P.K. all'insulina suina erano invertite, sebbene le stesse reazioni all'insulina bovina rimanessero positive.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01548086
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    Electronic Resource
    Kinetics of continuous cultivation (1969)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969), S. 19-36 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Kinetic, studies were made on continuous cultivation applying the theory of microbial cell growth that was derived previously by the authors introducing the concepts of critical concentration and coefficient of consumption activity. General equations for microbial cell concentration for continuous cultivation in continuous-stirred tank and tubular type reactors were derived theoretically. Productivity of cell mass in continuous cultivation was analyzed kinetically and the behavior of mutant populations in continuous cultivation is briefly discussed.
    Additional Material: 10 Ill.
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    URL: http://dx.doi.org/10.1002/bit.260110103
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  • 40
    Electronic Resource
    Electronic Resource
    Oxygen transfer in streptomyces fermentation broths (1969)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969), S. 605-622 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The oxygen transfer coefficient has been investigated in S. noursci and S. lavendulae fermentation broths obtained from fermentors of different operating volumes (61., 30001., 20,0001.). Fermentors had KLas values ranging from 1.0 to 17.0 min-1, calculated from sulphite oxidation rates. The dynamic measurement of the volumetric oxygen transfer coefficient. (KLa) has been performed in the different fermenting systems. As the fermentation progressed, especially in the first stages, KLa values have decreased in both fermentations and in each system of fermentors. In order to characterise the whole fermenting system an average KLas was calculated from the obtained KLa values. The average KLa grew with increasing KLas values and ranged from 0.03 to 3.72 min-l. Some factors possibly having an influence on the, change of KLa have been studied.The oxygen transfer coefficients of the broths have been measured in falling films and ranged from 0.05 to 0.4 cm min-1. The flow conditions have been characterized by Reynolds numbers of broths varying between 1.0 and 60.0. The average thickness of the falling films have been measured and plotted against Reynolds number. The Re+ which is the breaking-point of the plot increased as the fermentation proceeded. In the region of Re+ the values of the oxygen transfer coefficient increased rapidly. An approximate correlation could be established between the Re+ and the physical properties of fermentation broth.
    Additional Material: 12 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260110408
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  • 41
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    Increased oxygen mass transfer rates from single bubbles in microbial systems at low reynolds numbers (1969)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969), S. 647-681 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The presence of microorganisms have been shown to increase by over 40% the mass transfer rates from small oxygen bubbles at low Reynolds number flow. This increase was found to be due only to the microbe cells as inert particles disrupting the quasi-static liquid surface film surrounding the gas bubble and thus decreasing the gas-liquid interfacial resistance. The observed increase in oxygen mass transfer rates was not dependent on cell viability, no effect was noted due to cell-liquid interfacial resistance, nor was the phenomenon due to altering the physical properties of the liquid during cell propagation. These results were obtained in a unique plexiglass apparatus designed for observing under a microscope a small (0.4 mm dia.) stationary oxygen bubble collapsing into a flowing fluid. The oxygen bubble was injected by a small hypodermic needle and the fluid was suspensions of the yeast Candida intermedia, the bacterium Pseudomonas ovalis, 0.3μ alumina, as well as base points of cell free broth and pure water. Several well-known chemical inhibitors of oxidative phosphorylation were used to limit cell oxygen uptake. Calculations of oxygen mass transfer rates were compared with the semi-empirical model of Frössling, the circulating sphere model of Levich, and the rigid sphere concentration boundary layer model of Fried-lander, the latter two showing strong Reynolds number dependence that may be due to radial fluid motion.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260110411
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  • 42
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    The role of polyketides in secondary metabolite production by penicillia (1969)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969), S. 1135-1156 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The participation of polyketides in the biogenesis of natural products has long been bolstered by chemical analogies. Many isotopic tracer studies have validated the acetate-polymalonate route, via presumptive extended poly-β-carbonyl intermediates, to a variety of fungal metabolites. Though implicit as antibiotic precursors, the ephemeral polyketides have not been isolated, nor perhaps with the exception of acetoacetate, can oligoketides become incorporated intact into secondary metabolites. However, a prototypical oligoketides in its stable lactone form, methyltriacetic lactone (3, 6-dimethyl-l-hydroxy-2-pyrone), has been obtained from the tropolone-producing mold P. Stipitatum. A convenient synthesis of this metabolite, by methylation of triacetic lactone followed by partition chromatographic separation of the resultant positional isomers, has been devised. In an experiment with 14C-formate, it was shown that the hypothetical, enzyme-bound polyketide precursor to methyltriacetic lactone is probably involved in stipitatie arid formation, and that the origin of the “extra” methyl or methyl-derived carbons of both substances arises from the identical “C1” pool. Radioactive tracer experiments concerning the biogenesis of pulvilloric acid, a fairly unstable antibiotic substance produced by P. Pulvillorum, showed that its exocyclic carboxyl is formed following initial methyl transfer, whereas the ring system of the molecule is essentially acetate-polymalonate derived. In order to test the hypothesis that methyl-branched C14 polyketide precursors to pulvilloric acid exist and may become integrated into the fatty acid multienzyme complex, presumptive fatty acid congeners to pulvilloric acid such as. 1-methylmyristie, 4-methyllauric, or 2-methyllauric acids were sought. These substances were, however, absent from the mycelial fatty acid spectrum, as well as from the fatty acid moieties of a crystalline glyceridc mixture obtained from the beer. Alternative approaches to the detection or isolation of polyketides are discussed.
    Additional Material: 7 Ill.
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    URL: http://dx.doi.org/10.1002/bit.260110610
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    Activation of the biosynthesis of carotenoids by Blakeslea trispora (1969)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969), S. 1195-1210 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The research carried out by several scientists has made possible the industrial preparation of β-carotene by fermentation. A fungus, Blakeslea trispora, abundantly synthesizes carotenoids when its two opposite forms are cultivated together in a special fatty medium. When ionones or other natural substances are introduced into the culture, a very obvious increase in the biosynthesis of carotenoids, more specifically of β-carotene, is obtained.Our own work has shown that; (1) several synthetic products chemically related to β-ionone, such as 2,6,6-trimethyl-l-acetyleyelohexene, can advantageously replace either partially or totally the ionones as inductors of the biosysnthesis of β-carotene; (2) various nitrogen-containing substances when added to the culture medium can considerably enhance the biosysnthesis of carotenoids while sometimes very specically orienting it. Their action comes on top of that of the ionones or their substitutes; actually this action is unexplained.Thus certain amides, imides, lactams, hydrazides, or substituted pyradines, and in particular succinimide and isonicotinoylhydrazine, have produced a two or threefold increase in the quantity of β-carotene present in the culture media of Blackeslea trispora.Conversely some heterocyclic substances such as pyridine itself or imidazole totally inhibit the biosysnthesis of β-carotene but induce the production of very important quantities of lycopene.
    Additional Material: 18 Tab.
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    Measurement of heat evolution and correlation with oxygen consumption during microbial growthThis paper was presented in part at the 154th Meeting of the American Chemical Society, Chicago, III., Sept. 11, 1967. (1969)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969), S. 269-281 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A procedure for measuring the rate of heat production from a fermentation has been developed. The method is based on measuring the rate of temperature rise of the fermentation broth resulting from metabolism, when the temperature controller is turned off. The heat accumulation measured in this manner is then corrected for heat losses and gains. A sensitive thermistor is used to follow the temperature rise with time. This procedure is shown to be as accurate as previous methods but much simpler in execution. Using this technique, the rate of heat production during metabolism was found to correlate with the rate of oxygen consumption. Experiments were performed using bacteria (E. coli and B. subtilis), a yeast (C. intermedia), and a mold (A. niger). The substrates investigated included glucose, molasses, and soy bean meal. The proportionality constant for the correlation is independent of the growth rate, slightly dependent on the substrate, and possibly dependent On the type of organism growth. This correlation has considerable potential for predicting heat evolution from the metabolism of microorganisms on simple or complex substrates and providing quantitative parameters necessary for heat removal calculations.
    Additional Material: 5 Ill.
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    URL: http://dx.doi.org/10.1002/bit.260110302
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    An introduction to the symposium on hydrocarbon fermentation, third international fermentation symposiumThe symposium was held on Tuesday, September 3, at Rutgers University, New Brunswick, New Jersey. (1969)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969), S. 381-381 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260110309
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  • 46
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    Kinetics of microbial oxidation of n-heptane. I. Characterization of the process (1969)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969), S. 417-426 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Utilization of n-heptane by a Pseudomonad was studied in pilot-size butch cultures. Optimal pH and temperature were determined by a factorial design and a medium based upon mineral uptake rates was formulated. High cell yields were obtained by volatilizing heptane in the incoming air and thereby achieving good hydrocarbon dispersion. Hydrocarbon carried by effluent gases was recovered and recycled. In cultures where pH is not controlled, decrease in the electrolytic conductivity of the medium was found to be indicative of viable cells and was used in monitoring bacterial propagation. If not checked, increase in salinity in pH controlled cultures was found to affect cell production negatively. Viscosity changes were not very significant. Heptane to aqueous medium ratio was found to affect oxygen supply to the system due to higher dissolved oxygen concentrations associated with hydrocarbons.
    Additional Material: 4 Ill.
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  • 47
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    Regulation of biosynthesis of secondary metabolites. I. Biosynthesis of chlortetracycline and tricarboxylic acid cycle activity (1969)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969), S. 539-548 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: In the course of submerged cultivation of low-production and industrial production strains of Streptomyces aureofaciens, the activity of enzymes of the tricurboxylic acid cycle was studied. The activities of citrate synthase (EC 4.1.3.7), aconitate hydratase (EC 4.2.1.3), isocitrate dehydrogenase (EC 1.1.1.42), fumarate hydratase (EC 4.2.1.2), and malate dehydrogenase (EC 1.1.1.37) were estimated spectrophotometrically in cell-free preparations. In the growth phase, mainly the initial reactions of the cycle were active with both strains. In production-phase, the activities of enzymes in the low-production strain were 2-5 × higher than in the production strain. Benzylthioeyanate, at a concentration of 5 × l0-5M, stimulated chlortetracycline production of both strains with accompanying decrease in activity of the enzymes of the tricarboxylic acid cycle. The role of the tricarboxylic acid cycle in control of chlortetracycline biosynthesis is discussed.
    Additional Material: 1 Ill.
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    URL: http://dx.doi.org/10.1002/bit.260110402
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  • 48
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    Use of pH and pE measurements during methane biosynthesisPresented at the Third International Fermentation Symposium, Rutgers University, September 2-6, 1968. (1969)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969), S. 683-699 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Methane biosynthesis within the anaerobic ecosystem results from the diphasic conversion of complex organic substrates through a series of biologically mediated oxidation-reduction reactions which arc pH dependent. Routine parametric analysis of the products emanating from the corresponding acid and methane fermentation phases of the anaerobic digestion process provide a basis for determination of process condition and efficiency. Of these analyses, pH and electrode potential of pE are intensive parameters of considerable significance but for which interpretation is often difficult and misleading. Attention has been focussed on the analogy and distinction between pH and pE determinations and their application to the anaerobic ecosystem. Theoretical and practical limitations are discussed and experimental evidence presented which supports the possible use of pH and pE determinations for process evaluation and control.
    Additional Material: 3 Ill.
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    URL: http://dx.doi.org/10.1002/bit.260110412
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  • 49
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    The rheological effects of substrate-additives on fermentation yields (1969)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969), S. 725-730 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260110416
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  • 50
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    Methylcellulose effect on cell proliferation and glucose utilization in chemically defined medium in large stationary culturesPaper presented at 19th Annual Meeting of the Tissue Culture Association San Juan, Puerto Rico, June 1968. (1969)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969), S. 155-179 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Three different established strains of mammalian cells were grown in chemically defined medium in large cultures. The degree of proliferation of cells of an established strain from human skin in large stationary cultures was significantly greater in the presence of methylcellulose (medium NCTC 135M) than in its absence (medium NCTC 135). The relatively fragile cells of a derivative of monkey kidney LLCMK2 strain were carried in large stationary cultures through 11 transfer generations during 152 days. The presence of methylcellulose was associated with higher cell population levels, proliferation rates, and cell viability. Cells of this strain utilized glucose at an extremely high rate; during two representative periods the rate averaged 1.2 mg/106 cells/day in cultures on medium 135M and 1.9 mg in medium 135.In a 53-day experiment with mouse fibroblast 2071-L cells, the cells in suspension culture during the first 28 days went through the normal lag, logarithmic plateau, and initial decline phases in medium 135M, and then were transferred to large stationary cultures, where they proliferated for 7 days at uniformly high rates in both medium 135 and medium 135M. It appeared that cells of strain 2071-L in such stationary cultures had no need for Methocel as a protective agent. Glucose utilization rates while these cells were carried in large stationary cultures averaged 2-4 times the rates while they were in suspension cultures: about 0.8 and 0.2 mg/106 cells/day, repectively.
    Additional Material: 3 Ill.
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    URL: http://dx.doi.org/10.1002/bit.260110205
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    Composition of cell material and biological value of the cellular protein of a micrococcus strain grown on hydrocarbons (1969)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969), S. 409-416 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A Micrococcus cerificans strain was grown on simple media with n-hexadecane or gas oil as sole carbon sources. Samples of cellular material recovered from hexadecane or gas oil fermentations do not appear to differ significantly in their composition. The protein content varied from 68 to 75%. With the exception of sulfur amino acids the amino acid distribution compares favorably with the FAO standard reference protein.The biological value of cell protein recoveered from hexadecane fermentations was 67 (cascin, 70). In the case of gas oil grown cells, the cell material recovered had to be completely purified in order to improve its protein quality. After fully extraction of undersirable fraction with petroleum ether in a Soxhlet apparatus the biological value observed was 63.
    Additional Material: 1 Ill.
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    URL: http://dx.doi.org/10.1002/bit.260110312
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  • 52
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    Effect of alkylbenzenes on n-alkane utilization (1969)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969), S. 441-448 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: It has been shown that alkylbenzenes which do not support growth of Pseudomonas aeruginosa exert a stimulatory or inhibitory effect on the utilization of n-alkanes by this organism. The effect is a function or the number of methyl substitutions to the benzene ring and the concentration of aromatic hydrocarbon. The alkylbenzenes have been shown to exert the stimulatory and inhibitory effect, on all phases of growth. The data suggests different mechanisms involved in the inhibition by benzene and p-xylene but a similar mechanism by all of the stimulatory compounds. Warburg data demonstrates changes in the rate of oxygen uptake in the presence of trimethylbenzene at different stages of the fermentation. The release of water soluble material in the presence of trimethylbenzene suggests a permeability effect on the cells.
    Additional Material: 7 Ill.
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    URL: http://dx.doi.org/10.1002/bit.260110315
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    A laboratory-scale pulper for leafy plant material (1969)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969), S. 517-528 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A machine is described that, makes, from 2 to 3 kg samples of leaf, a pulp comparable to that made by the large-scale equipment used in leaf protein extraction. It is therefore suitable for use in agronomic experiments on leaf protein yield.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260110319
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  • 54
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    Introduction to the symposium on engineering aspects of fermentation, third international fermentation symposium (1969)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969), S. 581-581 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260110405
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  • 55
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    An improved french pressure cell (1969)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969), S. 711-718 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A description is given of a needle valve press for disrupting cells and microorganisms. The main features of the design are reproductibility and ease of control of the needle valve settings due to absence of screw threads, case of dismantling and cleaning, and the availability of a wide range of alternative piston and cylinder sizes.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260110414
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  • 56
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    The foams of fermentation broths. I. Some parameters of the foaming of fermentation media (1969)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969), S. 701-710 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The stability of foam formed during fermentation is decisively affect ed by the nature of the nutrient media used. In froth-flotation models, (a) the foam formation time, characteristic of the tendency to foam, and (b) foam subsistence time, characteristic of the stability of foams formed, have been studied. With the utilization of these two parameters, the stability of foam from aqueous solution of several surface active components of nutrient media has been noted as a function of concentrations. Further, but, without attempting completeness, the viscosity enhancing effect of carbohydrate components, and the effect of the subsistence time of their foam, upon the stability of foam have been studied together with the correlation between “standing” time after sterilization and tendency to foam. Taking soy-bean meal as a model, the stability of foam films in function of pi I, at constant concentration, has been studied. It seems that though a proper control of the factors mentioned, nutrient media with a low tendency of foaming can be formulated.
    Additional Material: 5 Ill.
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    URL: http://dx.doi.org/10.1002/bit.260110413
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  • 57
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    Masthead (1969)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969) 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260110501
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  • 58
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    Model identification of the biochemical oxidation process (1969)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969), S. 731-743 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A fairly general model of the biochemical oxidation, which takes into account the activity of microorganisms, is presented. Parameters of the model have been determined by fitting the model to available experimental data through the use of a straightforward gradient technique.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260110502
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  • 59
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    Semi-continuous culture and monitoring system for temperature-synchronized Euglena (1969)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969), S. 745-756 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A temperature-synchronized, semi-continuous culture and monitoring system is described, with results from use of the system for autotrophically growing Euglena. Outflow from the culture vessel consists of measured samples taken automatically at 2-hour intervals and fixed for later counting. Inflow is by siphon feed, which restores the culture level after each sampling. The interpretation of growth curves obtained from such cultures is discussed from the viewpoint, of division synchrony and cell cycle studies, and some general comparisons are made between batch and continuous cultures for such studies.
    Additional Material: 4 Ill.
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    URL: http://dx.doi.org/10.1002/bit.260110503
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  • 60
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    Growth of Streptococcus faecalus in dense culture (1969)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969), S. 805-841 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A fermentation system was designed and constructed to study the growth characteristics of microorganisms at low and high cell concentrations. The technique used to develop high cell densities utilized a rotating microfiltration unit to permit the removal of cell-free product from the fermenter. The fermenter volume and the filter were contained in a single unit composed of a series of concentric cylinders. Annuli contained the fermenter volume while the second outermost cylinder supported a microfiltration membrane. Feed to the system was pumped at constant rates, and the internal pressure built up to a value, which would effect the required filtration rate. The system was operated batchwise and continuously with and without filtration.The anaerobie growth characteristics of Streptococcus faccalus were determined at 37°C and pH 7.0 for batch, continuous, and continuous with filtration modes of operation. The growth characteristics were unchanged when the cell density was increased. Changes in cell yield peer model of glucose consumed were clearly illustrated during thee course of single run by operating the fermenter in the unsteady state with filtration. No consumption of glucose for developed was 40% packed cell volume, a value 45 times larger than could be grown in simple batch culture.
    Additional Material: 12 Ill.
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    URL: http://dx.doi.org/10.1002/bit.260110509
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  • 61
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    Predator-prey relationships in a model for the activated process (1969)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969), S. 887-907 
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    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The primary objective of this paper was to develop a mathematical description for the food chain, Because of the interdependence of the elements in this food chain, continuous oscillations among the variables are possible. A set of three differential equations was obtained to describe the above system in a continuously fed stirred tank reactor. The differential equations obtained were examined to characterize the possible types of solutions. A limit, cycle solution was obtained for some values of the system parameters.
    Additional Material: 9 Ill.
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    URL: http://dx.doi.org/10.1002/bit.260110514
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  • 62
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    Introduction to the symposium on engineering aspects of fermentation (second part) (1969)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969), S. 909-909 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260110515
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  • 63
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    A continuous, multistage tower fermentor. I. Design and performance tests (1969)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969), S. 927-943 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The design of a continuous column fermentor with a multiple staging effect is described. The column is divided into four compartments by horizontal perforated plates and is provided with a central agitator shaft driving an impeller in each compartment. A tube at the center of each plate forms a liquid seal around the shaft and also acts as a “downcomer.”The fermentor is normally operated with counter-current flow of gas and medium. Fresh medium is added to the top stage and product is withdrawn from the bottom.The effect of plate and agitator design on fermentor performance was studied in terms of factor such as oxygen transfer rate, gas holdup, and interstage mixing. By proper choice of the design parameters, the fermentor was made to approximate a perfect four-stage cascade in terms of reactor performance.Preliminary experiments were performed with air-water systems, but a more realistic picture of fermentor performance was obtained in experience involving propagation of Escherichia coli. Data for business and substrate concentrations in each stage confirmed the staging effect of the apparatus. The fermentor operated in a stable manner for periods of more than two weeks.
    Additional Material: 6 Ill.
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    URL: http://dx.doi.org/10.1002/bit.260110517
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  • 64
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    Performance of a perforated plate column as a multistage continuous fermentor (1969)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969), S. 911-926 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Microorganisms were continuously cultivated in multistage column consisting of ten perforated plate sections to which medium and air were supplied concurrently from the bottom.At steady state the cell concentration in the various stages was gradationally differentiated from the bottom to the top in the direction of medium flow. RNA content per unit cell concentration at each sage was determined. The cells in the lower stages were higher in RNA content than those from the upper stages. Wash out was observed to occur in the column at dilution rates which do not result in wash out in a single stage chemostat system.A study of the flow characteristics revealed that the overall performance of the plate column was equivalent to that of a multistage system, when hole diameter and hole area to column cross sectional area ratio were properly selected. This was true even in highly aerated conditions. These results indicated that the perforated plates in the column hindred intermixing through the plates, and that each stage functioned as an independent stirred vessel. Industrial and research application of this type fermentor was discussed.
    Additional Material: 12 Ill.
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    URL: http://dx.doi.org/10.1002/bit.260110516
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  • 65
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    A flow cell photometer for bacterial growth monitoring (1969)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969), S. 1005-1010 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A flow cell photometer is described with automatic cleaning of the photometric cell, denasimetric separation of air bubbles and precipitates, and a constant sensitivity from 0 to 10 mg/ml of bacterial dry weight.
    Additional Material: 4 Ill.
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    URL: http://dx.doi.org/10.1002/bit.260110521
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    An improved, totally automated antibiotic bioassay machine (1969)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969), S. 1011-1025 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A number of improvements have been made in a totally-automated antibiotic bioassay machine previously described. The new machine accepts unmeasured, untreated, opaque suspensions of fermentation beers three times faster (120 samples per hour) and supplies printed potencies sooner (in just over two hours). Whereas the original machine employed a self-cleaning filter and used disposable two milliliter beakers, this version involves a batch-dialysis scheme for effecting sample purification, and provides for automated cleaning of incubation chambers.In operation, a measured, portion of thoroughly-mixed fermentation beer is automatically diluted and transferred into one side of an incubation chamber, the two halves of which are separated by a dialysis membrane. The other half is filled with inoculated media. During the two hour incubation at 37°, dialyzable antibiotic limits growth of the inoculum in proportion to its concentration. After incubation, the turbidity of the inoculum is simultaneously read by an online computer and plotted on a strip chart recorded. The computer suplies printed potency values and sample identification on site, while the recording provides the operator with an analog record of turbidity. Fiber optics are employed in the turbidmetric readout, and an electric typewrite provides the printout.
    Additional Material: 18 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260110522
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    Large scale production of transfer ribonucleic acids from E. coli K-12 MO7 (1969)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969), S. 1055-1070 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: An engineering-scale procedure for the recovery of 300-400 g batches of mixed transfer ribonucleic acids is described. Semicontinuous growth of E. coli K-12 MO7 yielded 77 kg of harvested cells in four days. Phenol extraction and ethanol precipitation recovered a crude tRNA material that was further purified by DKAE-cellulose chromatography in runs of 1 × 106 A260 units each on a 6 × 30 in. column using a 240 1, gradient elution. The purified tRNAs were partially concentrated and resolved into three groups.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260110603
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  • 68
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    Simultaneous gas-liquid interfacial mass transfer and uptake by small particles (1969)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969), S. 1071-1087 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The presence of active small particles, such as bacterial cells, in a liquid will affect the rate of gas-liquid interfacial mass transfer. A theoretical analysis of the situation is presented in this article.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260110604
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  • 69
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    The production, isolation, and characterization of a grisein-like sideromycin complex (1969)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969), S. 1111-1123 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Streplomyces griscus var. X-2455 produces an antibiotic complex which is active in vitro against a number of gram-positive and gram-negative bacteria and in mice against systemic infections caused by K, pneumoniae and D, pneumoniac. In view of the favorable chemotherapeutic index and the broad in vitro spectrum of crude concentrates, isolation of the pure antibiotic complex and the individual constituents was undertaken. The antibiotics referred to as Ho 5-2667, Ro 7-7730, and Ho 7-7731 can be differentiated by tle, ultraviolet light absorption spectra, and in vitro antibacterial activities. They all contain iron and may be classified as sideromycins.From antibiotic concentrates an antibacterially inactive substance was isolated and identified as N-acetyltyramine.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260110608
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  • 70
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    The design, construction, and characteristics of a new long-lived steam sterilizable oxygen electrode (1969)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969), S. 323-335 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A steam sterilizable oxygen electrode for fermentor use is described. The electrode has a silver cathode, lead anode, phosphate electrolyte, and a membrane of a fluorinated ethylene-propylene copolymer film (FEP.).The electrode has a linear response to partial pressure of oxygen from 1.5 × 10-2 to 103 mm Hg.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260110305
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  • 71
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    The kinetics of β-galactosidase attached to porous cellulose sheets (1969)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969), S. 363-380 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The properties of β-galactosidase attached to cellulose and DEAE-cellulose sheets arc described. Those insoluble enzyme derivatives obey the Michael-Menten relationship but, the measured kinetic parameters are very dependent on the flow conditions. The results of long-term stability tests are given.
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260110308
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  • 72
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    Microbial transformation of hydrocarbons. II. Growth constants and cell composition of microbial cells derived from n-alkanesF. Wagner, W. Zahn, and U. Bühring, “1-Hexadeceno, an intermediate in the microbial oxidation of n-hexadecane in rivo and in vitro,” Angew. Chem., Intn. Edit., 6, 359(1967). (1969)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969), S. 393-408 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Cultivation of Norcardia sp., Mycobacterium phlei, and Candida lipolytica in inorganic salt solution containing n-alkanes C10-C20 as solo carbon and energy source was investigated. Generation times of 0.5-7.0 hr were typical during the exponential growth phase. The final cell concentrations (dry weight) were usually 9-26 g/l with n-alkane mixtures ranging from n-decane through n-eicosane. A linear dependence was found between the production of cell mass and the consumption of n-alkanes. The rest concentration of n-alkanes in the cell mass is in all experiments smaller than 0.5% (w/w). Cell yields were Ysub 60-142% and for Ye 50-97% based on n-alkane utilization. In one case, with the Nocardia NBZ 23, the substrate specifity on hydrocarbons and on a n-alkane mixture C10-C20 was studied. The cell mass recovered from the fermentations contained 47.8-57.7% carbon, 5.6-9.95% nitrogen, 7.2-9.4% hydrogen, 35-62% crude protein, and 6-36% lipid. Cellular protein and lipid synthesized by an organism is influenced by the type of nitrogen source. The amino acid, glucosamine, muramic acid, 2,6-diaminopimelinic acid, and fatty acid distribution in organisms grown on n-alkanes compared with a corresponding fermentation on glucose as sole carbon source were also estimated.
    Additional Material: 1 Ill.
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    URL: http://dx.doi.org/10.1002/bit.260110311
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  • 73
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    Growth models of cultures with two liquid phases. I. Substrate dissolved in dispersed phase (1969)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969), S. 449-466 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Mathematical models which can be used to describe batch growth in fermentations with two liquid phases are developed for systems in which the growth limiting substrate is dissolved the dispersed liquid phase. In the models, the possibilities of growth occurring at the surface of the dispersed phase and in the continuous phase are considered. It is assumed that the composition of the dispersed phase is such that substrate utilization from it causes little of no change, in interfacial area. Three special cases are examined. In the first, it is assumed that all growth occurs at the surface of the dispersed phase. In the second and third, it is assumed that growth occurs both at the interface and in the continuous phase. The second case assumes that substrate equilibrium is continuously established between the two phases while the third assumes substrate consumption in the continuous phase is limited by rate of transport of substrate to that phase. Comparison of the first model with available experimental data shows good agreement between model and data.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260110316
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  • 74
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    A laboratory-scale press for extracting juice from leaf pulp (1969)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969), S. 529-538 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A press is described with which loads of up to 1 ton can be applied quickly to 1 kg quantities of leaf pulp spread over 450 cm2. The juice extracted is similar in quantity and quality to that extracted by large-scale equipment. Evidence is presented that the conditions of pressing are so uniform that the yields of juice are consistent.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260110320
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  • 75
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    A steam-sterilizable electrode of the galvanic type for the measurement of dissolved oxygen (1969)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969), S. 549-560 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: An oxygen probe is developed, using the principle of the silver-lead galvanic cell, covered with a plastic membrane. It is steam sterilizable while filled with water; after sterilization, water is easily exchanged for the electrolyte, potassium bicarbonate. Flat plastic membranes up to a thickness of about 0.1 mm can be applied. The membrane is secured and stretched in a special way, which guarantees a leakproof seal. The outer shape of the probe has a tulip-form and consists of polished stainless steel, except for the electrode face. The electrode is suitable for laboratory and industrial use and its components are completely standardized.Almost linear response for O2-partial pressures up to atmospheric is obtained with 0.001 in. and 0.002 in. FEP-Teflon membranes. 90% response time is of the order of 10-15 sec for the 0.001 in. membrane, Residual current is almost negligible (〈 1 μA).
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260110403
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  • 76
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    Masthead (1969)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969) 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260110101
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  • 77
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    Use of the glucose oxidase system to measure oxygen transfer rates (1969)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969), S. 1-18 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: This investigation used the glucose oxidase system to simulate oxygen transfer rate in fermentation broths. It was demonstrated that the fungal preparation contained sufficient lactonase activity so that D-glucono-δ-lactone did not accumulate and that the rate of production of gluconic acid was proportional to the oxygen uptake rate. Enzyme concentrations of 1.5-2 g/1 were found adequate to determine oxygen absorption rates in shake flasks while maintaining the dissolved oxygen concentration of low levels. The apparent Michaelis constant for oxygen, Km(O2), was found to be 27% saturation with air; this value along with experimentally determined uptake rates could be used to calculate dissolved oxygen concentration in lieu of using a dissolved oxygen probe. Enzyme concentrations of 5 g/l were sufficient to give linear acid production and low dissolved oxygen concentrations in a bench-scale fermenter with no foaming or enzyme deactivation. The method is considered more valid and easier to employ than previously utilized techniques such as sulfite oxidation. Extension of the system to evaluating aeration effectiveness and scaleup of fermentation equipment is discussed.
    Additional Material: 11 Ill.
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    URL: http://dx.doi.org/10.1002/bit.260110102
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  • 78
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    Studies of fungal growth and intermediary carbon metabolism under steady and non-steady state conditions (1969)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969), S. 785-804 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The physiology of Aspergillus nidulans strain 224 has been studied under conditions of batch- and glucose-limited chemostat-culture and the effect of different steady state growth rates and dissolved oxygen tensions (DOT) examined. Measurements of the specific activities of selected glucose enzymes, the extent of oxygen uptake inhibition by glycolytic inhibitors, and radiorespirometric analyses were made in order to follow the variations in glucose catabolism, which occurred under these conditions. Greatly increased activity of the hexosemonophosphate (HMP) pathway was found during: (i) exponential growth of batch cultures; (ii) at near maximum specific growth rates (μ = 0.072 hr-1) (DOT = 156 mm Hg); and (iii) at low DOT levels (〈30 mm Hg) (μ = 0.050 hr-1) in chemostat cultures. These changes in glucose eatabolism have been discussed in terms of the biosynthetic demands of the fungus under the influence of changing growth pressures. Preliminary studies also have been made of transition state behavior following stepwise alteration of the DOT. A new steady state was established after 4-5 culture doublings during which period an “overshoot” in HMP pathway activity occurred; these kinetics are indicative of a derepression of certain glucose enzymes. Low molecular weight phenols are synthesized during the exponential phase in batch cultures and these are further metabliized to a major secondary metabolite, melanin, at the onset of stationary phase conditions. The kinetics of tyrosinase production in steady state chemostats differs from those that might be predicted for an enzyme associated solely with secondary metabolism. A primary physiological role for this oxidase in Aspergillus nidulans has been postulated.
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260110508
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  • 79
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    Regulation of nitrogen levels for heterogeneous populations of sewage origin grown in completely mixed reactors (1969)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969), S. 79-98 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Heterogeneous populations of sewage origin were grown continuously at, dilution rates from 1/12 hr-1 to dilute-out (1/1 hr-1) using glucose (1000 mg/l) as carbon source and three concentrations of NH3-N as the nitrogen source (COD:N = 70:1, 40:1, and 25:1). The effects of nitrogen level and growth rate (dilution rate) on substrate removal, biological solids production, cellular carbohydrate and protein, and NH4+-N in the effluent were examined. It was found that the optimum level of nitrogen supplementation for the synthetic nitrogen-deficient waste employed should not be based solely on the desired effluent quality with respect to COD removal but should include due consideration of reactor detention time (or dilution rate) and the allowable (or desirable) level of nitrogen leakage in the effluent.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260110107
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  • 80
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    Citric acid from ferrocyanide-treated blackstrap molasses (1969)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969), S. 103-105 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 2 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260110109
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  • 81
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    Masthead (1969)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969) 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260110201
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  • 82
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    Application of continuous oxidative assimilation and endogenous protein synthesis to the treatment of carbohydrate wastes deficient in nitrogen (1969)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969), S. 53-65 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A synthetic waste (with glucose as carbon source) devoid of a source of nitrogen was purified in a laboratory scale pilot plant by a new modification of the activated sludge process. The process makes use of a separate carbon assimilation (oxidative assimilation) phase and an endogenous phase in which ammonia is added to a portion of the settled sludge and non-nitrogenous products stored in the cells in the assimilation phase are converted to protein. It was found that sludge so treated, when recycled to the assimilation tank, could carry out continuous oxidative assimilation of the waste. Various COD:N ratios were studied. At the highest, 70:1, 90% purificaton efficiency was achieved.
    Additional Material: 5 Ill.
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    URL: http://dx.doi.org/10.1002/bit.260110105
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  • 83
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    L-asparaginase synthesis by Escherichia coli B (1969)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969), S. 1211-1225 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: We have studied the influence of strain of organism, temperature, and medium on the production of the antileukemic intracellular enzyme L-asparaginase by E. coli B grown in shaken flasks. Five strains of E. coli B exhibited wide differences in their capacities to synthesize the EC-2 form of L-asparaginase active against leukemia. For the most productive strain, when grown in a casein hydrolysate medium, maximal production of L-asparaginase occurred at 25°C. At this temperature, the organism required glycerol, glucose, or other mono-saccharides to synthesize L-asparaginase. Synthesis was stimulated when glycerol was used in place of glucose, but not in its presence. The effect of glycerol on L-asparaginase synthesis was most evident when the cells were grown at 37°C, rather than at 25°C. With 0.25% glucose, cells had a specific activity of 409 I.U./g; with glycerol cells had a specific activity of 553 I.U./g. At 25°C, both cell and L-asparaginase synthesis were increased by the use of 0.25% glycerol resulting in only a slight increase in specific activity of the cells. The addition of zinc, copper, manganese, iron, L-asparagine, L-glutamine, or L-aspartic acid had no effect on L-asparaginase synthesis in the casein hydrolysate medium. L-aspartic acid (10-2 M) enhanced L-asparaginase synthesis in a synthetic medium that lacked these metals or L-asparagine, L-glutamine, or L-aspartic acid; cells grown under these conditions had a specific activity of 90 I.U./g.In the casein hydrolysate medium, cell morphology was correlated with temperature of incubation.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260110615
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  • 84
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    Transformation of steroids by mixed cultures (1969)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969), S. 1255-1270 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Some results of our studios on transformation of steroids by mixed culture fermentation are presented in this paper. Arthrobacter simplex was paired in turn with each of the following: Streptomyces roseochromogenes, Curvularia lunata, Absidia coerulea, and Aspergillus ochraceus. The steroid substrates examined for multiple transformation were 16α-hydroxy-cortexolone, 16α-hydroxy-cortexolone 16,17-acetonide, 9α-fluorohydrocortisone, 9α-fluorohydrocortisone 21-acetate, and 9α-fluorohydrocortisone 21-hemisuccinate. The effects of media, steroid substrate, and microbial interaction in a mixed culture on the induction and repression of steroid transforming enzymes were unique to each case studied. The reaction mechanism of the multiple steroid transformation was also found to vary from one mixed culture system to another. Two different reaction mechanisms were observed, namely, consecutive and parallel. In the former, one of the two enzymatic reactions always preceded the other, while in the latter, two different enzyme reactions occurred simultaneously, thereby giving rise to two different intermediates. Multiple transformation of steroids by a single step mixed culture fermentation has potential economic advantages.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260110619
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  • 85
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    Cell growth and population activity of Saccharomyces cerevisiae in two-stage continuous cultivation (1969)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969), S. 853-862 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Continuous culture in a cascade of vessels with the addition of supplemental nutrients to any stage permits adjustment of the physiological state of the culture in each stage to best achieve a desired performance goal. The yeast Saccharomyces cerevisiae in two-stage continuous cultivation was selected as a model system. With conditions in the first stage held constant- at a selected glucose concentration in the feed stream, dilution rate for the second stage was varied. Cell numbers, dry weight, glucose concentration, respiration coefficient, and titers of several enzymes were determined. The seed rate was defined as the ratio of glucose concentration in the feeds to stage 1 and to stage 2. At low seed rates, the calculated specific growth rate in the second stage was proportional to dilution rate. At higher seed rates, the specific growth rate based on dry weight behaved differently from that based on cell numbers, and the dependence on dilution rate was not linear.
    Additional Material: 5 Ill.
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    URL: http://dx.doi.org/10.1002/bit.260110511
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  • 86
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    Growth models of cultures with two liquid phases. III. Continuous cultures (1969)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969), S. 489-515 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Some mathematical models, which have been used to describe batch growth in fermentations with two liquid phases present, are used to predict the behavior of continuous fermentations in a chemostat. Two types of dispersed systems are considered in this investigation. In the first, type, it is assumed that the composition of the dispersed phase is such that, increased substrate utilization results in a decreased substrate concentration with no change in the interfacial area. In the second type of system, the dispersed phase is assumed to be pure substrate; therefore, the substrate concentration in the dispersed phase remains constant but the interfacial area is affected by changes in dilution rate. Three special cases are examined for each type of system in order to examine the effect of the interfacial area, the phase equilibrium constant, and the mass transfer coefficient on system performance. Comparison of two of the models with available experimental data shows fair agreement, between model and data.
    Additional Material: 21 Ill.
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    URL: http://dx.doi.org/10.1002/bit.260110318
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  • 87
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    Design and physical characteristics of a multistage, continuous tower fermentor (1969)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969), S. 945-966 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A multistage tower laboratory fermentor has been constructed consisting of eight compartments separated by sieve plates. Flow of substrate and air is concurrent from the bottom to the top of the column. It, was hoped that this system could be used to reproduce, simultaneously on a continuous basis, eight distinct phases of a batch growth curve. It was believed that the extent of batch curve simulation would depend upon the character of hydraulic mean residence time of broth in the column and in the individual compartments. The expected relationship did not occur. Rather it was found that growth in the column involved residence time characteristics not only for the fluid but also for the microorganisms, and for the growth limiting substrate. Depending upon the column operation, these could be distinct and different.The purpose of this investigation was to study the residence time distribution (RTD) of the continous (fluid) and dispersed (microorganisms) phases for model systems as well as for a yeast fermentation. Various degrees of flow nonideality, i.e., fluid blackflow and dispersed phase sedimentation, were noticed. The former seems to be due to interaction of the concurrent gas and liquid flow; it is particularly dependent upon void area of the sieve plate holes. Sedimentation is probably a function of plate design as well as cell size and density. It wa concluded that for a particular plate design the gas hold-up wass controlled by superficial air velocity and was the main parameter governing the differences between dispersed and continous phase(Rt1). This conclusion was supported by a computeraided styudy utilizing a mathematical model of fluid flow to fit the growth kinetics and cell distribution observed experimentally throughout the fermentor.Some advantages of foam control in the tower fermentor by surface active compounds are mentioned. Also, suggestions are made for carrying out fermentations that have two liquid phases, such as a hydrocarbon fermentation. The possibility of closely approximating plug-flow conditions in the multistage tower fermentor, a necessary condition for batch growth simulation, is discussed from a practical point of view.
    Additional Material: 17 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260110518
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  • 88
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    Biosynthesis of chloramphenicol (1969)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969), S. 1125-1134 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The current knowledge concerning the biosynthesis of chloramphenicol is discussed. Cultures of Streptomyces sp. 3022a fed 14C-shikimie acid incorporated the label to the same extent into phenylalanine, tyrosine, and chloramphenicol. Of possible precursors of the phenylpropanoid nucleus of this antibiotic only p-aminophenylalanine and DL-threo-p-amino phenylserine specifically labeled chloramphenicol. On the basis of these results a pathway for the biosynthesis of chloramphenicol is presented. The lack of specific incorporation of 15N-nitrogen from a competitive feeding experiment in which both l5N-nitrate and 14N-DL-serine were fed to growing cultures suggests that both the amido- and the nitro-nitrogen atom present in this antibiotic are derived from a common pool. Studies on the enzyme, DAHP synthetase, show that in streptomyces sp. 3022a it is not subject to feed back inhibition by either phenylalanine, tyrosine, or chloramphenicol.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260110609
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  • 89
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    Aromatization of 9α-hydroxy-19-norandrostenedione by Arthrobacter simplex (1969)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969), S. 1183-1194 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: 9α-Hydroxy-19-norandrostenedionc (9α-hydroxy-Δ4-estrene 3, 17-dione) (IV) was prepared by fermentation of 19-norandroslenedione with Corynespora melanis or Norcardia restriclus. When incubated with a growing culture of Arthrobacter simplex or its acetone-dried cells, IV was converted to 9α-hydroxyestronc (VII) and 9-keto-9, 10-secoestrone (VI). 9α-Hydroxyestrone undergoes spontaneous as well as enzymic dehydration to form Δ9(11)-estrone (IX). Both VI and IX have been isolated and identified as such while VII was isolated as its 3-acetate.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260110613
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  • 90
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    Electronic Resource
    Enzymatic brominatioin of the thiazole ring (1969)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969), S. 1227-1232 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Bromination of the thiazole ring by the enzyme chloroperoxidase, produced by the fungus Caldariomyces fumago, was demonstrated. Both 2-acetoacetamido-4-methylthiazole and 2-acetamidothiazole were brominated on C-5 of the thiazole ring in the presence of chloroperoxidase, bromide, and hydrogen peroxide in 0.06 M Phosphate solution at pH 3.0. No reaction occurred in the absence of enzyme.
    Additional Material: 2 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260110616
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  • 91
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    Physiology of, an enzyme production by, plant cell cultures (1969)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969), S. 1247-1254 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Red kidney bean (Phascolus vulgaris) cells, derived from roof, callus, were grown in suspension culture in shake flasks and in laboratory fermentors using batch and continuous batch culture techniques. The medium contained casein hydrolysate, sucrose, inorganic salts, vitamins, and growth hormones. In continuous batch culture yields of up to 171 g wet weight, (8.5 g dry weight) per liter were obtained in 7 days. Organic nitrogen was used preferentially. Growth on nitrate was considerably slower than on organic nitrogen sources. Indole acetic and naphthalene acetic acids were not essential for good growth of the cells whereas kinetin and 2, 4-D were. The optimum pH for growth was about p11 4.5. The presence of amylase and peroxidase was detected in culture filtrates. Amylase activity was low in either the presence or the absence of starch in the medium. Peroxidase production could be related directly with growth of the culture. Maximum peroxidase yield, as measured by the guaiacol method and expressed as horse radish peroxidase, was 1.25 × 10-8 M.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260110618
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  • 92
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    The relationship between critical oxygen level and antibiotic synthesis of capreomycin and cephalosporin C (1969)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969), S. 583-592 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: This paper illustrates the type of conclusions which may be reached by the use of an electrode for measuring oxygen tension in fermentation broths. The critical oxygen level, based on oxygen uptake, and the minimum oxygen tension necessary for maximum antibiotic synthesis for two commercial antibiotic fermentations, capreomycin and cephalosporin C, were studied. Capreomycin yields were depressed at an oxygen tension which was slightly below the critical oxygen level. Cephalosporin C synthesis was depressed at an oxygen tension higher than the critical oxygen level.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260110406
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  • 93
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    Investigation of some methods for increasing the digestibility in vitro of microalgae (1969)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969), S. 37-51 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: In order to increase the availability of the cell bound protein in Scenedesmus algae, mechanical, enzymatic, and chemical methods of degrading the cell wall structure were investigated.Mechanical treatment involved the use of a ball-mill. The algae suspension together with glass beads was milled in a water-cooled chamber equipped with rotating disks. The enzyme tested was a cellulolytic enzyme (Meicelase) and the chemical employed was hydrogen peroxide.In the ball-mill experiments a complete disintegration was achieved ina disintegrator, working with batches. Trails wwere also performed with a continuous disintegrator and the depedence of disintegration on bead size and flow rate was studied. The disintegration determined by microscropic cell count was compared to the increase of the pepsin digestibility.The meicelase treatment caused a slight increase of the pepsin digestibility, as measured after 3 hr pepsin incubation. No increase of the pepsin disgestibility could be detected with hydrogen peroxide treatment.After the ball-mill disintegration 95% of contaminating bacteria were killed and yields of extractable proteins were higher. The capacity of availble continuous ball-mills is such that they could be used on a pilot-plant scale and the energy cost of disintegration would be of the same magnitude as that of separation.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260110104
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  • 94
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    Correlation of lags in the utilization of mixed sugars in continuous fermentation (1969)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969), S. 99-102 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260110108
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  • 95
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    Studies on the relationship between specific growth rate and concentration of nitrogen source for heterogeneous microbial populations of sewage origin (1969)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969), S. 67-78 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Batch experiments were run using heterogeneous populations to determine whether a hyperbolic equation of the type suggested by Monod could be used to depict the relation between specific growth rate, μ, and NH3-N concentration when ammonia N was the growth-limiting nutrient. The heterogeneous populations employed were developed from sewage seed grown on glucose at various levels of nitrogen and various dilution rates in completely mixed continuous flow reactors. It was found that the hyperbolic function could be used. Values of μm in the range of 0.4-0.7 hr-1 were observed, and values of Ks, in general, ranged from 1.5 to 4.0 mg/l. Variation in the values of these growth “constants” did not follow any discernible pattern related to past growth history (i.e., COD:N ratio or dilution rate at which the cells were previously grown).
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260110106
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  • 96
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    Electronic Resource
    Automatic electrode selection device for monitoring ph (1969)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969), S. 1027-1032 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260110523
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  • 97
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    Electronic Resource
    Growth potential of a variation of eagle's minimum essential medium for spinner cultures (1969)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969), S. 263-266 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260110209
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  • 98
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    Electronic Resource
    Masthead (1969)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969) 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260110301
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  • 99
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    Electronic Resource
    Kinetics of fermentation processes (1969)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969), S. 293-321 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Kinetic studies on fermentation processes were made and a general equation of production rate was newly presented applying the kinetic theory on mierobial cell growth which was reported previously by the authors.l,2 Equations for product concentration in fermentation time courses were derived by developing mathematically the general equation of production rate, and characteristic properties of fermentation processes were clarified. Some examples of fermentations were analyzed kinetically using the new kinetic theory. The calculated values of product, and cell concentrations were in good agreement with the observed values.
    Additional Material: 22 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260110304
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  • 100
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    Electronic Resource
    The conversion of benzyl penicillin to 6-aminopenieillanie acid using an insoluble derivative of penieillin amidase (1969)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 11 (1969), S. 337-348 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Penicillin amidase was extracted from Escherichia coli ATCC 9637, grown on phenylacetic, acid and glutamate, and purified by fractional ion with streptomycin sulphate, ammonium sulphate and polyethylene glycol, followed by chromatography on DEAE-cellulose. The purification factor was 100-200 × and the overall yield was about 115%. The enzyme was chemically attached to derivatives of cellulose and the kinetics of these insolubilized penicillin amidase preparations was investigated.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260110306
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