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  • 1
    Publication Date: 2020-12-11
    Description: A solution generating technique is developed for $D=5$ minimal supergravity with two commuting Killing vectors based on the $G_2$ U-duality arising in the reduction of the theory to three dimensions. The target space of the corresponding 3-dimensional sigma-model is the coset $G_{2(2)}/(SL(2,R)\times SL(2,R))$. Its isometries constitute the set of solution generating symmetries. These include two electric and two magnetic Harrison transformations with the corresponding two pairs of gauge transformations, three $SL(2,R) \; S$-duality transformations, and the three gravitational scale, gauge and Ehlers transformations (altogether 14). We construct a representation of the coset in terms of $7\times 7$ matrices realizing the automorphisms of split octonions. Generating a new solution amounts to transforming the coset matrices by one-parametric subgroups of $G_{2(2)}$ and subsequently solving the dualization equations. Using this formalism we derive a new charged black ring solution with two independent parameters of rotation.
    Keywords: ddc:530
    Language: English
    Type: reportzib , doc-type:preprint
    Format: application/pdf
    Format: application/postscript
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  • 2
    Book
    Book
    Boston :Birkhäuser,
    Title: Sampling, wavelets, and tomography /
    Contributer: Benedetto, John , Zayed, Ahmed I.
    Publisher: Boston :Birkhäuser,
    Year of publication: 2003
    Pages: p. cm
    Series Statement: Applied and computational harmonic analysis
    ISBN: 0-8176-4304-4 , 3-7643-4304-4
    Type of Medium: Book
    Language: English
    Keywords: Hamonic analysis ; Wavelets (Mathematics) ; Fourier analysis ; Sampling (Statistics) ; Tomography
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Journal of computational analysis and applications 2 (2000), S. 333-371 
    ISSN: 1572-9206
    Keywords: sampling theorem ; Cauchy's integral formula ; Poisson's summation formula ; Fourier analysis ; complex analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Notes: Abstract First we show that several fundamental results on functions from theBernstein spaces $$B_\sigma ^p $$ (such as Bernstein's inequality andthe reproducing formula) can be deduced from a weak form of the classicalsampling theorem. In §3 we discuss the mutual equivalence of thesampling theorem, the derivative sampling theorem and a harmonic functionsampling theorem. In §§4–6 we discuss connections between thesampling theorem and various important results in complex analysis andFourier analysis. Our considerations include Cauchy's integral formula,Poisson's summation formula, a Gaussian integral, certain properties ofweighted Hermite polynomials, Plancherel's theorem, the maximum modulusprinciple, and the Phragmén–Lindelöf principle.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Documenta ophthalmologica 101 (2000), S. 233-246 
    ISSN: 1573-2622
    Keywords: binocular vision ; Fourier analysis ; strabismus ; temporal frequency ; visual evoked potential (VEP)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The purpose of this work was to study the second harmonic in the steady state pattern visual evoked potential (ssVEP) to various stimulus frequencies in subjects with normal and defective binocularity. ssVEPs were elicited by 4 c/deg sinusoidal gratings, with temporal frequencies ranging from 5 to 20 Hz (exp. 1) and 15 to 27.5 Hz (exp. 2). Responses were Fourier analysed and power and phase of the second harmonic to stimulus frequency were measured. For power, binocular enhancement in a bimodal fashion was found both in normals and in subjects with defective binocularity. The power with binocular stimulation was significantly higher in the normal group in the high frequency domain. Latency, estimated from the phase-frequency function, was longer in the group with defective binocularity, but this was statistically significant only for the high frequency domain. The results suggest that a visual system with normal binocular function can follow a stimulus with high temporal frequency more accurately than a system with disturbed binocularity.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Medical & biological engineering & computing 38 (2000), S. 558-561 
    ISSN: 1741-0444
    Keywords: Hydrodynamic simulation ; Pulse duplicator ; Cardiovascular system ; Atrial fibrillation ; Fourier analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract Heart valve replacements are often associated with cardiac pathologies, but valvular prostheses are still tested in vitro under the same physiological conditions as for a healthy young man. Therefore a new mock circulatory system of the left heart, the dual activation simulator (DAS), has been built. The DAS allows atrial and ventricular dynamics to be controlled with pumps that activate anatomically shaped silicon models of the cavities. The mitral flow is a two-peak waveform. The E/A ratio can be changed, and the A-wave can be suppressed to simulate, for instance, atrial fibrillation. The cardiac rhythm and the mean flow-rate can be changed at will. The ability of the DAS to reproduce physiological flow is assessed by computation of the aortic input impedance and by harmonic analysis of left ventricular and atrial pressures. It allows the behaviour of valve prostheses to be studied in various conditions of concern to clinicians and can be a useful tool for engineers to improve valve prostheses or validate diagnostic tools such as 3D colour Doppler. The DAS and its capacities are described.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1436-2813
    Keywords: Key words Lung cancer ; Operation ; Hemophilia ; Recombinant DNA ; Coagulation factor VIII
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Hemophilia A is a sex-linked recessive hereditary disease that is relatively rare and the number of patients with this disorder who undergo major surgery is limited. Although replenishing coagulation factors can allow hemophiliac patients to undergo similar surgery to that performed for patients without hemophilia, there have been few reports on major surgery and none on the resection of lung cancer in patients with hemophilia A. We recently performed completion pneumonectomy of the left lung in a 70-year-old man with hemophilia A, for squamous cell carcinoma in the residual left lung. The administration of a recombinant DNA coagulation factor VIII preparation allowed this operation to be successfully carried out. This case serves to demonstrate that the recombinant DNA coagulation factor VIII preparation described may enable us to safely perform major surgery on hemophiliac patients, since there is no risk of viral infection or any other adverse effects, such as deterioration of immunocompetence or hemolysis, which are occasionally encountered with human plasma-derived preparations.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Annals of combinatorics 3 (1999), S. 13-25 
    ISSN: 0219-3094
    Keywords: 60C05 ; 60G99 ; algebraic enumeration ; binary sequences ; set partitions into even-size blocks ; restricted set partitions ; finite residue classes modulo three ; moment ; centered moment ; cumulant ; bounds ; Fourier analysis ; Möbius inversion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Notes: Abstract A probability set function is interpretable as a probability distribution on binary sequences of fixed length. Cumulants of probability set functions enjoy particularly simple properties which make them more manageable than cumulants of general random variables. We derive some identities satisfied by cumulants of probability set functions which we believe to be new. Probability set functions may be expanded in terms of their cumulants. We derive an expansion which allows the construction of examples of probability set functions whose cumulants are arbitrary, restricted only by their absolute values. It is known that this phenomenon cannot occur for continuous probability distributions. Some particular examples of probability set functions are considered, and their cumulants are computed, leading to a conjecture on the upper bound of the values of cumulants. Moments of probability set functions determined by arithmetical conditions are computed in a final example.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Monatshefte für Mathematik 127 (1999), S. 43-53 
    ISSN: 1436-5081
    Keywords: Key words: Homogeneous trees ; Fourier analysis ; wave equation ; 1991 Mathematics Subject Classification: 43A85 ; 35L05; 39A12
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Notes: Abstract.  Let ? be a homogeneous tree, ℒbe the Laplace operator of ?, and b be the bottom of its L 2 spectrum. Let u be a solution of the (modified) wave equation on ?. Using Fourier analysis on ? we show that the energy of u is asymptotically divided into equal potential and kinetic parts.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Journal of pharmacokinetics and pharmacodynamics 27 (1999), S. 23-43 
    ISSN: 1573-8744
    Keywords: pharmacodynamics ; indirect response models ; chronobiology ; cortisol ; fluticasone propionate ; Fourier analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Six mathematical functions to describe the chronobiology of cortisol concentrations were assessed. Mean data from a dose-proportionality study of inhaled fluticasone propionate were fitted with an indirect response model using various biorhythmic functions (single cosine, dual ramps, dual zero-order, dual cosines, and Fourier series with 2 and n-harmonics) for production rate. Data with known parameters and random variation were also generated and fitted using the ADAPT II program. Fitted parameters, model estimation criteria, and runs tests were compared. Models with preassigned functions: the dual ramps, the dual zero-order and the dual cosines provide maximum and minimum times for cortisol release rate, were suitable for describing asymmetric circadian patterns and yielding IC50 values. Fourier analysis differs from the other methods in that it uses the placebo data to recover equations for cortisol secretion rate rather than by postulation. Nonlinear regression for Fourier analysis, instead of the L 2 -norm method, was useful to characterize the baseline cortisol data but was restricted to a maximum of two harmonics. Apart from the single cosine function, which predicts symmetrical cortisol concentrations, all methods were satisfactory in describing the baseline and suppressed cortisol concentrations. On the other hand, Fourier series with L 2 -norm produced the best unbiased estimate for baseline patterns. The Fourier method is flexible, accurate, and can be extended to other drug-induced changes in normal periodic rhythms.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Urological research 27 (1999), S. 83-96 
    ISSN: 1434-0879
    Keywords: Key words Genitourinary malignancies ; Recombinant DNA ; Gene cloning ; DNA chip technology ; Diagnostics ; Gene therapy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Cancer, including genitourinary malignancy, is a consequence of accumulated genetic aberrations in genes involved in crucial regulatory pathways. The result is a deregulation of cellular behaviour, leading to neoplastic transformation, uncontrolled cell proliferation and acquisition of metastatic ability. The development and perfection of techniques in the field of recombinant DNA technology, gene cloning, (differential) analysis of gene expression, and sequencing of genes and proteins have provided a wealth of information about the genetic aberrations associated with cancer development. This “recombinant DNA and gene cloning” technology and the recently developed DNA chip technology may provide new molecular diagnostic tools. Furthermore, the technology of gene cloning in combination with the progress in in vivo gene delivery techniques offers new treatment modalities, like gene therapy, additional to conventional therapies. This review is intended to provide a general introduction to the fundamentals or strategies of recombinant DNA and gene cloning techniques as a basis for understanding the rapidly expanding range of new diagnostic and therapeutic opportunities. Some illustrative examples are provided, addressing basic and biomedical research and possible clinical applications in genitourinary oncology.
    Type of Medium: Electronic Resource
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  • 11
    Electronic Resource
    Electronic Resource
    Springer
    Documenta ophthalmologica 99 (1999), S. 69-82 
    ISSN: 1573-2622
    Keywords: evoked responses ; ERG ; Fourier analysis ; pattern ERG ; steady-state ; VEP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Fourier analysis is a powerful tool in signal analysis that can be very fruitfully applied to steady-state evoked potentials (flicker ERG, pattern ERG, VEP, etc.). However, there are some inherent assumptions in the underlying discrete Fourier transform (DFT) that are not necessarily fulfilled in typical electrophysiological recording and analysis conditions. Furthermore, engineering software-packages may be ill-suited and/or may not fully exploit the information of steady-state recordings. Specifically: • In the case of steady-state stimulation we know more about the stimulus than in standard textbook situations (exact frequency, phase stability), so `windowing' and calculation of the `periodogram' are not necessary. • It is mandatory to choose an integer relationship between sampling rate and frame rate when employing a raster-based CRT stimulator. • The analysis interval must comprise an exact integer number (e.g., 10) of stimulus periods. • The choice of the number of stimulus periods per analysis interval needs a wise compromise: A high number increases the frequency resolution, but makes artifact removal difficult; a low number `spills' noise into the response frequency. • There is no need to feel tied to a power-of-two number of data points as required by standard FFT, `resampling' is an easy and efficient alternative. • Proper estimates of noise-corrected Fourier magnitude and statistical significance can be calculated that take into account the non-linear superposition of signal and noise. These aspects are developed in an intuitive approach with examples using both simulations and recordings. Proper use of Fourier analysis of our electrophysiological records will reduce recording time and/or increase the reliability of physiologic or pathologic interpretations.
    Type of Medium: Electronic Resource
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  • 12
    Electronic Resource
    Electronic Resource
    Springer
    Hydrogeology journal 7 (1999), S. 139-150 
    ISSN: 1435-0157
    Keywords: Key words groundwater hydraulics ; transport system ; flow system ; Fourier analysis ; dispersion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences
    Description / Table of Contents: Résumé L'analyse des hydrosystèmes souterrains est basée sur le concept de systèmes hiérarchiques d'écoulement souterrain. La topographie de la surface piézométrique, qui est étroitement liée à celle de la surface du sol, est le facteur principal de l'emboîtement hiérarchique des écoulements souterrains, gouvernés par la gravité, ce qui fait apparaître des systèmes d'écoulement de différentes échelles en étendue et en profondeur de pénétration. Le concept de système d'écoulement est extrêmement utile pour analyser les échelles spatiales et temporelles et leurs relations mutuelles. Les équations de base correspondant à l'échelle du laboratoire sont étendues à des échelles régionales, plus vastes. L'utilisation de la méthode de Fourier met mieux en valeur l'idée originale de Tóth de systèmes d'écoulement commandés par la topographie. De cette façon, les différentes échelles spatiales de la nappe sont séparées naturellement, en donnant une expression simple pour la profondeur de pénétration du système d'écoulement souterrain. Cette décomposition fournit aussi la relation entre les échelles spatiale et temporelle. Dans une approche analogue à celle des systèmes d'écoulement, des masses d'eaux de qualités différentes peuvent être appelées "systèmes de transport". Des études de terrain, une modélisation numérique à micro-échelle sur des domaines à macro-échelle et la théorie de la dispersion stochastique indiquent qu'entre des systèmes soumis à un transport en régime permanent, les interfaces sont relativement minces. Les interfaces sont beaucoup plus minces que les zones de mélange relativement étendues prédites par l'approche conventionnelle de l'ingénierie pour la macro-dispersion, dans laquelle on applique des longueurs de macro-dispersion, indépendant du temps et relativement étendues. Une approche d'ingénierie alternative, relativement simple, est présentée. Pour la macro-dispersion de la propagation de panaches de soluté, le terme alternatif de dispersion donne les mêmes résultats que l'approche d'ingénierie conventionnelle et donne des résultats corrects pour le transport en régime permanent.
    Abstract: Resumen El análisis de los sistemas de flujo se basa en el concepto de modelos jerárquicos de aguas subterráneas. La topografía del nivel freático, estrechamente relacionada con la topografía de superficie, es uno de los factores principales en la continuidad jerárquica del flujo subterráneo gravífico, dando lugar a sistemas de flujo con distintos órdenes de magnitud en lo que respecta a extensión lateral y profundidad. El concepto de sistemas de flujo es extremadamente útil para el análisis de las escalas espacial y temporal y de sus interrelaciones. Las ecuaciones básicas deducidas a escala de laboratorio se extienden a escalas regionales. Mediante análisis de Fourier se llega al esquema original de Tóth de sistemas de flujo dominados por la topografía. De esta manera, las diferentes escalas espaciales del nivel freático quedan separadas de manera natural, lo que conduce a una expresión simple para la profundidad de penetración en un sistema de flujo. Esta descomposición conduce además a una relación ente las escalas espacial y temporal. De manera análoga a los sistemas de flujo, los cuerpos de agua de distinta calidad química pueden llamarse "sistemas de transporte". Tanto los estudios de campo como los modelos numéricos regionales con discretización a microescala, o la teoría estocástica de la dispersión indican que, para los sistemas con transporte estacionarios, las interfaces son bastante delgadas; más delgadas, por ejemplo, que las predichas por un tratamiento convencional de la macrodispersión, donde se utilizan valores relativamente grandes e independientes del tiempo. El estudio de la macrodispersión de penachos contaminantes se realiza mediante un modelo alternativo simple, donde el término alternativo de dispersión da los mismos resultados que los modelos convencionales.
    Notes: Abstract  Flow-system analysis is based on the concept of hierarchical groundwater flow systems. The topography of the water table, which is strongly related to the topography of the land surface, is a major factor in the hierarchical nesting of gravity-driven groundwater flow, resulting in flow systems of different orders of magnitude in lateral extent and depth of penetration. The concept of flow systems is extremely useful in the analysis of spatial and temporal scales and their mutual relationships. Basic equations on the laboratory scale are extended to larger, regional scales. Making use of Fourier analysis further develops Tóth's original idea of topography-driven flow systems. In this way, the different spatial scales of the water table are separated in a natural way, leading to a simple expression for the penetration depth of a flow system. This decomposition leads also to the relationship between spatial and temporal scales. Analogous to flow systems, water bodies with different water quality may be called 'transport systems.' Field studies, numerical micro-scale modeling over macro-scale domains, and stochastic dispersion theory indicate that between systems with steady transport, the interfaces are relatively thin. The interfaces are much thinner than the relatively large mixing zones predicted by the conventional engineering approach to macrodispersion, in which relatively large, time-independent macrodispersion lengths are applied. A relatively simple alternative engineering approach is presented. For macrodispersion of propagating solute plumes, the alternative dispersion term gives the same results as the conventional engineering approach and gives correct results for steady-state transport.
    Type of Medium: Electronic Resource
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  • 13
    Electronic Resource
    Electronic Resource
    Springer
    The journal of Fourier analysis and applications 4 (1998), S. 67-91 
    ISSN: 1531-5851
    Keywords: 43A30 ; 43A65 ; 43A85 ; 68T10 ; 68U10 ; Fourier analysis ; irreducible representations of the groupSL(2, ℂ) ; harmonic analysis onSL(2, ℂ) ; projectively invariant classification of patterns ; projectively adapted pattern representation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Notes: Abstract Among all image transforms, the classical (Euclidean) Fourier transform has had the widest range of applications in image processing. Here its projective analogue, given by the double cover groupSL(2, ℂ) of the projective groupPSL(2, ℂ) for patterns, is developed. First, a projectively invariant classification of patterns is constructed in terms of orbits of the groupPSL(2, ℂ) acting on the image plane (with complex coordinates) by linear-fractional transformations. Then,SL(2, ℂ)-harmonic analysis, in the noncompact picture of induced representations, is used to decompose patterns into the components invariant under irreducible representations of the principal series ofSL(2, ℂ). Usefulness in digital image processing problems is studied by providing a camera model in which the action ofSL(2, ℂ) on the complex image plane corresponds to, and exhausts, planar central projections as produced when aerial images of the same scene are taken from different vantage points. The projectively adapted properties of theSL(2, ℂ)-harmonic analysis, as applied to the problems, in image processing, are confirmed by computational tests. Therefore, it should be an important step in developing a system for automated perspective-independent object recognition.
    Type of Medium: Electronic Resource
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  • 14
    ISSN: 1617-4623
    Keywords: Key words Restriction endonuclease ; Methylase selection ; Gene expression ; DNA methylation ; Recombinant DNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The genes encoding the ApaLI (5′-G^TGCAC-3′), NspI (5′-RCATG^Y-3′), NspHI (5′-RCATG^Y-3′), SacI (5′-GAGCT^C-3′), SapI (5′-GCTCTTCN1^-3′, 5′-^N4GAAGAGC-3′) and ScaI (5′-AGT^ACT-3′) restriction-modification systems have been cloned in E.␣coli. Amino acid sequence comparison of M.ApaLI, M.NspI, M.NspHI, and M.SacI with known methylases indicated that they contain the ten conserved motifs characteristic of C5 cytosine methylases. NspI and NspHI restriction-modification systems are highly homologous in amino acid sequence. The C-termini of the NspI and NlaIII (5′-CATG-3′) restriction endonucleases share significant similarity. 5mC modification of the internal C in a SacI site renders it resistant to SacI digestion. External 5mC modification of a SacI site has no effect on SacI digestion. N4mC modification of the second base in the sequence 5′-GCTCTTC-3′ blocks SapI digestion. N4mC modification of the other cytosines in the SapI site does not affect SapI digestion. N4mC modification of ScaI site blocks ScaI digetion. A DNA invertase homolog was found adjacent to the ApaLI restriction-modification system. A DNA transposase subunit homolog was found upstream of the SapI restriction endonuclease gene.
    Type of Medium: Electronic Resource
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  • 15
    Electronic Resource
    Electronic Resource
    Springer
    Journal of mathematical imaging and vision 8 (1998), S. 99-130 
    ISSN: 1573-7683
    Keywords: superposition of periodic structures ; moiré effect ; Fourier analysis ; geometry of numbers ; spectrum support ; dense spectrum ; discrete spectrum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Notes: Abstract A new approach is presented for investigating the superposition of any number of periodic structures, and the moiré effects which may result. This approach, which is based on an algebraic analysis of the Fourier-spectrum using concepts from the theory of geometry of numbers, fully explains the properties of the superposition of periodic layers and of their moiré effects. It provides the fundamental notations and tools for investigating, both in the spectral domain and in the image domain, properties of the superposition as a whole (such as periodicity or almost-periodicity), and properties of each of the individual moirés generated in the superposition (such as their profile forms and intensity levels, their singular states, etc.). This new, rather unexpected combination of Fourier theory and geometry of numbers proves very useful, and it offers a profound insight into the structure of the spectrum of the layer superposition and the corresponding properties back in the image domain.
    Type of Medium: Electronic Resource
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  • 16
    Electronic Resource
    Electronic Resource
    Springer
    Mathematical geology 29 (1997), S. 953-962 
    ISSN: 1573-8868
    Keywords: time series ; Fourier analysis ; cyclolhems ; Pennsylvanian stratigraphy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Mathematics
    Notes: Abstract Several stratigraphic sequences in the Upper Carboniferous (Pennsylvanian) in Kansas (Midcontinent, USA) were analyzed quantitatively for periodic repetitions. The sequences were coded by lithologic type into strings of datasets. The strings then were analyzed by an adaptation of a one-dimensional Fourier transform analysis and examined for evidence of periodicity. The method was tested using different states in coding to determine the robustness of the method and data. The most persistent response is in multiples of 8–10 ft (2.5–3.0 m) and probably is dependent on the depositional thickness of the original lithologic units. Other cyclicities occurred in multiples of the basic frequency of 8–10 with persistent ones at 22 and 30 feet (6.5–9.0 m) and large ones at 80 and 160 feet (25–50 m). These levels of thickness relate well to the basic cyclothem and megacyclothem as measured on outcrop. We propose that this approach is a suitable one for analyzing cyclic events in the stratigraphic record.
    Type of Medium: Electronic Resource
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  • 17
    Electronic Resource
    Electronic Resource
    Springer
    Il nuovo cimento della Società Italiana di Fisica 18 (1996), S. 807-821 
    ISSN: 0392-6737
    Keywords: Plasma flow ; magnetohydrodynamics ; PACS 52.70 ; Plasma diagnostic techniques and instrumentation ; Fourier analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Summary Disruptive instabilities were investigated in the small-tokamak TBR-1 during the application of resonant helical magnetic fields created by external helical windings. Indications were found that the main triggering mechanism of the disruption was the rapid increase of them=2/n=1 mode which, apparently after reaching a certain amplitude, interacts with other resistive modes: the internal 1/1 mode in the case of a major disruption, or with higherm components, as the 3/1 or 4/1, in the case of minor disruptions. After the coupling, the growth of the associated islands would create a chaotic field line distribution in the region between the corresponding rational magnetic surfaces which caused the gross particle transport and, finally, destroyed the confinement. In addition, investigations on higherZ eff discharges in which a mixture of helium and hydrogen was used resulted in much more unstable plasmas but apparently did not alter the basic characteristics of the disruptions.
    Type of Medium: Electronic Resource
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  • 18
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical medicine and bioethics 17 (1996), S. 279-314 
    ISSN: 1573-1200
    Keywords: Recombinant DNA ; transgenesis ; patents ; human life forms
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Philosophy
    Notes: Abstract The rationale of patents on transgenic organisms leads to the startling notion of the human qua infringement. The moral reasons by which we may tenably reject such notion are not conclusive as to human life forms outside the body. A close look at recombinant DNA experimentation reveals ingenious processes, but not entities that the body lacks. Except for artificial genes, the genes of biotechnology are found on chromosomes, albeit nonconsecutively, and their uninterrupted transcripts appear in messenger RNA. An enhanced form of protection for ingenious processes, the “human methods patent,” is proposed and defended as a replacement for product patents. The proposed patent would pertain to biotechnology manufacturing and genetic intervention in somatic and germ cells. A counterpart could govern nonhuman life forms. It is argued that compulsory licensing protections should be a condition of such patent. Contrary to the conservative assumption that statutory sobriquets suffice, the reckoning of what qualifies as a patentable ingenious process will continue to require systematic scientific guidance.
    Type of Medium: Electronic Resource
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  • 19
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 19 (1996), S. 327-332 
    ISSN: 0935-6304
    Keywords: Capillary GC ; Separation performance ; Fourier analysis ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A simple procedure is presented for determining separation performance of HRGC analysis of multicomponent mixtures. The procedure is based on the computation of Autocovariance Function (EACVF) from the digitized experimental chromatogram. Graphic inspection of the EACVF plot permits easy computation of the width value of the single component peak, σ; from the EACVF value at t=O the number of component in the mixture, m, can be simply derived. From these two basic quantities all the other chromatographic performance attributes can be calculated. The consistency of the procedure is tested for different chromatograms and compared with the more complex EACVF fitting method. Several features of the multicomponent chromatogram, overloading effects included, are directly detected.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
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  • 20
    Electronic Resource
    Electronic Resource
    Springer
    Computational complexity 5 (1995), S. 205-221 
    ISSN: 1420-8954
    Keywords: Lower-bound ; complexity ; Fourier analysis ; 68Q25 ; 68Q99
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science
    Notes: Abstract We use Fourier analysis to get general lower bounds for the probabilistic communication complexity of large classes of functions. We give some examples showing how to use our method in some known cases and for some new functions. Our main tool is an inequality by Kahn, Kalai, and Linial, derived from two lemmas of Beckner.
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  • 21
    ISSN: 1432-0983
    Keywords: Recombinant DNA ; Filamentous fungi ; 5-fluoro-orotic acid ; Homologous transformation system
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A homologous gene transfer system for Aspergillus awamori for site-specific integration is described, based on two components. First, a defined A. awamori pyrG mutant strain constructed by a selection strategy for gene-replacement in fungi. Second, a vector with a homologous pyrG selection marker containing a defined mutation at a site different from that of the mutations in the pyrG gene of the defined mutant strain. Defined mutation in the A. awamori pyrG gene, isolated from a genomic library by heterologous hybridisation with the A. niger pyrG gene as a probe, were introduced by specifically altering sequences at restriction sites in the coding region of the gene. After transformation of the A. awamori wild-type strain with vectors containing these mutated pyrG genes, and selection for 5-fluoro-orotic acid resistance (5-FOAR), on the average 60% of the 5-FOAR colonies originated from replacement of the wild-type pyrG gene by the mutated pyrG allele. After transformation of a mutant strain, carrying a mutation near the 5′ end of the pyrG gene with vectors containing a mutation near the 3′ end of the pyrG gene, 35% of the resulting transformants contained one copy of the vector at the pyrG locus.
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  • 22
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    Psychopharmacology 117 (1995), S. 162-165 
    ISSN: 1432-2072
    Keywords: Tardive dyskinesia Instrumental assessment ; Movement disorders Digital image processing ; Fourier analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The present study was performed to investigate the reliability and validity of a new device for the assessment of oral dyskinesias by means of digital image processing. Twenty schizophrenic patients with tardive dyskinesia (TD) and ten healthy controls were studied. In patients instrumental scores were compared to different clinical rating scale scores. Measurements were repeated after 2 weeks under the same circumstances to assess test-retest stability. Instrumental scores discriminated well between normal subjects and dyskinetic patients and correlated significantly with clinical ratings (r=0.63). The test-retest correlations showed a correspondence not larger than 40%, detecting thus the fluctuation of the TD intensity over time. These results suggest that our device is a reliable and easy to handle technique for the assessment of TD. Furthermore, the ability of the device to analyze the frequency distribution of movements makes it a useful tool for the quantitative and qualitative analysis of TD.
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  • 23
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    Annals of biomedical engineering 23 (1995), S. 772-786 
    ISSN: 1573-9686
    Keywords: Spatial co-occurrence matrix ; Fourier analysis ; Image processing ; Textural analysis ; Protein distribution ; Superfusion ; Aging ; Entropy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Technology
    Notes: Abstract Many pathologies are age-related, e.g., cardiovascular disease generally occurs in midlife and cancer later in life. This suggests that aging predisposes the body to pathology. Plasma protein spatial distribution images of rat mesentery extracellular matrix (ECM) show texture due to the ECM structures, and there is an age-related decrease in tissue protein that may be related to matrix structure changes. The objective of this study was to compare changes in protein image texture under two conditions: superfusion with normal saline solution and aging. The decrease in soluble protein concentration during superfusion is a washout process, while the mechanism for the age-related decrease in tissue protein is unknown. Therefore, effects of aging and superfusion on tissue protein image texture were compared. Spatial co-occurence matrix and Fourier analysis techniques have been used for texture evaluation. Superfused images showed a more uniform protein texture. There were gradual age-related changes in image texture parameters. Entropy increased with age from 140 to 630 days, indicating that protein distribution became more disorganized. The results suggest that changes in protein image texture are due to age-related alterations in matrix structure because removing only protein by superfusion had opposite effects on texture parameters.
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  • 24
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    Documenta ophthalmologica 90 (1995), S. 143-155 
    ISSN: 1573-2622
    Keywords: Electroretinogram ; Fourier analysis ; Naka-Rushton equation ; Obesity surgical therapy ; Oscillatory potentials ; Vitamin A deficiency
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract New possible causes and unexplored aspects of the electroretinogram were evaluated in a case of vitamin A deficiency secondary to surgical therapy for morbid obesity. The Naka-Rushton equation, applied to the scotopic b-wave, demonstrated the quantal catch reduction caused by the loss of rhodopsin in the outer segment of photoreceptors. Study of the Fourier analysis of the photopic 20-Hz response suggested a primary involvement of the external retinal layers, with an indirect alteration of the inner layer. The electroretinogram oscillatory potentials showed alterations explained by the involvement of their generators related to the primary photoreceptor lesion.
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  • 25
    ISSN: 1619-1560
    Keywords: heart rate ; diabetic neuropathies ; Fourier analysis ; parasympathetic nervous system ; sympathetic nervous system ; regression analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract A total of 24 subjects with type I insulin-dependent diabetes mellitus were studied. Cardiac parasympathetic function was measured by supine heart rate variability (HRV) in the respiratory frequency 0.10–0.50 Hz and the sympathetic index was measured as the ratio of HRV between 0.055 and 0.098 Hz to that between 0.004 and 0.5 Hz. Factors assessing diabetic control and complications, and factors unrelated to diabetes but possibly influencing HRV, were recorded. Association with depressed HRV was assessed with correlation, and prediction of depressed HRV was determined with multiple regression. Factors associated with depressed HRV but not independently predictive were renal dysfunction and elevated thyroid stimulating hormone. Elevated glycosylated haemoglobin was not significantly correlated with depressed HRV. Four factors (presence of diabetic retinopathy, male gender, duration of diabetes and increasing age) were significant in the regression and sufficed to predict 81% of the sample variance. The relative weights (β) were −0.65, 0.40, −0.40 and 0.26, respectively. Supine sympathetic index was not sufficient to demonstrate sympathetic dysfunction. It is proposed that the regression model may be used to identify patients likely to have cardiac parasympathetic autonomic dysfunction.
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  • 26
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    Annals of biomedical engineering 23 (1995), S. 482-490 
    ISSN: 1573-9686
    Keywords: Fourier analysis ; Transparency ; Opacity ; Scaling ; Cataract
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Technology
    Notes: Abstract Power law analysis provides a quantitative method for characterization of spatial fluctuations in the cellular microstructure of the ocular lens. In the power law analysis, Fourier components of the spatial fluctuations are computed, and the relationship between the amplitude, A, and spatial frequency, f, of the components is defined by a power law function: |A|2≈(1/f)β. The exponent of the function, β, defines the scaling of the amplitude of the Fourier components as a function of spatial frequency. We performed two-dimensional power law analysis on electron micrographs of lens cells ranging from transparent to opaque. We identified two values of power law exponent, β, for the spatial fluctuations of all lens cells, one for low-and a second for high-spatial frequencies. In the low-spatial frequency region, the value of β was in the range of 0.53 to 1.33, for transparent and opaque cells. In the high-spatial frequency region, the value of β increased from 2.78 for transparent lens cells to 3.60 for opaque lens cells. The power law analysis provides a new method for quantitative characterization of the spatial fluctuations in the microstructure of transparent and opaque lens cells.
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  • 27
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    International Journal for Numerical Methods in Engineering 38 (1995), S. 371-397 
    ISSN: 0029-5981
    Keywords: Helmholtz equation ; least squares ; Galerkin method ; Fourier analysis ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: In this paper a Galerkin least-squares (GLS) finite element method, in which residuals in least-squares form are added to the standard Galerkin variational equation, is developed to solve the Helmholtz equation in two dimensions. An important feature of GLS methods is the introduction of a local mesh parameter that may be designed to provide accurate solutions with relatively coarse meshes. Previous work has accomplished this for the one-dimensional Helmholtz equation using dispersion analysis. In this paper, the selection of the GLS mesh parameter for two dimensions is considered, and leads to elements that exhibit improved phase accuracy. For any given direction of wave propagation, an optimal GLS mesh parameter is determined using two-dimensional Fourier analysis. In general problems, the direction of wave propagation will not be known a priori. In this case, an optimal GLS parameter is found which reduces phase error for all possible wave vector orientations over elements. The optimal GLS parameters are derived for both consistent and lumped mass approximations. Several numerical examples are given and the results compared with those obtained from the Galerkin method. The extension of GLS to higher-order quadratic interpolations is also presented.
    Additional Material: 20 Ill.
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  • 28
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    International Journal for Numerical Methods in Fluids 20 (1995), S. 1003-1022 
    ISSN: 0271-2091
    Keywords: incompressible flow ; mixed formulations ; stabilized algorithms ; Fourier analysis ; finite elements ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: We present a method to assess the stability of pairs of interpolation spaces for mixed formulations. The method is based on a straightforward calculation of the eigenvalues of the discrete matrices through Fourier decomposition in plane waves and is intended to give, via straightforward numerical computations, a sharper determination of stability than the well-known ‘patch test’ of Zienkiewicz et al. Special attention is devoted to the study of stability and accuracy of equal-order interpolations.
    Additional Material: 34 Ill.
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  • 29
    ISSN: 1432-0827
    Keywords: Amelogenin ; Expression ; Enamel ; Recombinant DNA ; Tooth
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Abstract A mouse cDNA encoding a 180 amino acid amelogenin was subcloned into the pET expression plasmid (Novagen, Madison, WI) for production in Escherichia coli. A simple growth and purification protocol yields 20–50 mg of 95–99% pure recombinant amelogenin from a 4.5-liter culture. This is the first heterologous expression of an enamel protein. The expressed protein was characterized by partial Edman sequencing, amino acid composition analysis, SDS-PAGE, Western blotting, laser desorption mass spectrometry, and hydroxyapatite binding. The recombinant amelogenin is 179 amino acids in length, has a molecular weight of 20,162 daltons, and hydroxyapatite binding properties similar to the porcine 173 residue amelogenin. Solubility analyses showed that the bacterially expressed protein is only sparingly soluble in the pH range of 6.4–8.0 or in solutions 20% saturated with ammonium sulfate. The purified protein was used to generate rabbit polyclonal anti-amelogenin antibodies which show specific reaction to amelogenins in both Western blot analyses of enamel extracts and in immunostaining of developing mouse molars.
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  • 30
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    Journal of statistical physics 76 (1994), S. 1005-1043 
    ISSN: 1572-9613
    Keywords: Wave propagation ; spatiotemporal bifurcation theory ; biorthogonal decomposition ; Fourier analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract By using biorthogonal decompositions, we show how uniformly propagating waves, togehter with their velocity, shape, and amplitude, can be extracted from a spatiotemporal signal consisting of the superposition of various traveling waves. The interaction between the different waves manifests itself in space-time resonances in case of a discrete biorthogonal spectrum and in resonant wavepackets in case of a continuous biorthogonal spectrum. Resonances appear as invariant subspaces under the biorthogonal operator, which leads to closed sets of algebraic equations. The analysis is then extended to superpositions of dispersive waves for which the (Fourier) dispersion relation is no longer linear. We then show how a space-time bifurcation, namely a qualitative change in the spatiotemporal nature of the solution, occurs when the biorthogonal operator is a nonholomorphic function of a parameter. This takes place when two eigenvalues are degenerate in the biorthogonal spectrum and when the spatial and temporal eigenvectors rotate within each eigenspace. Such a scenario applied to the superposition of traveling waves leads to the generation of additional waves propagating at new velocities, which can be computed from the spatial and temporal eigenmodes involved in the process (namely the shape of the propagating waves slightly before the bifurcation). An eigenvalue degeneracy, however, does not necessarily lead to a bifurcation, a situation we refer to as being self-avoiding. We illustrate our theoretical predictions by giving examples of bifurcating and self-avoiding events in propagating phenomena.
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  • 31
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    Journal of cancer research and clinical oncology 120 (1994), S. 454-464 
    ISSN: 1432-1335
    Keywords: Xeroderma pigmentosum fibroblasts ; DNA repair ; Recombinant DNA ; cDNA libraries ; Phage λgt10 ; pBluescript vector ; In vitro transcripts ; Differential hybridization ; Mitochondrial neuromyopathies ; Genetic defects ; Uracil-DNA glycosylase ; Glyceraldehyde-3-phosphate dehydrogenase ; Lactate dehydrogenase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Differential hybridization was used to detect repair defects in xeroderma pigmentosum (XP) that are not amenable to current analyses. cDNA libraries were constructed from cytoplasmic RNA of normal and XP fibroblast strains (complementation groups A and D) and analyzed for differential gene expression. More than 40000 λgt10 cDNA clones were differentially screened with in vitro transcripts made from cDNA in the pBluescript vector. Six differential clones were detected in the libraries of the XP group A and D strains which caused stronger or weaker signals when probed with transcripts from XP strains than with those from the normal strains. Two clones coded for mitochondrial genes: mitochondrial 16 S rRNA and ATPase 6L. Overexpression of mitochondrial genes in XP may indicate that functions of the ATP-generating system are impaired since such functions are intensified whenever they become insufficient, for example as a consequence of DNA damage. It is tempting to assume that abnormal mitochondria are one of the causes for the neurological malfunctions in XP. Furthermore, densitometric analysis of Northern blots revealed that mRNA of lactate dehydrogenase, chain M, was less abundant in four XP group A strains (extent of reduction: 70%) and in two XP group D strains (extent of reduction: 58%). Enzyme activity was also diminished. In addition, mRNA of the gene for glyceraldehyde-3-phosphate dehydrogenase was less expressed in the same XP group A and D fibroblast strains investigated (reduction in both complementation groups: 50%). Both glycolytic enzymes have nuclear functions apart from their role in sugar metabolism. Lactate dehydrogenase, chain M, is identical to a helix-destabilizing protein; it is closely associated with chromatin and unfolded DNA, suggesting a role in DNA synthesis and transcription. The 37-kDa subunit of glyceraldehyde-3-phosphate dehydrogenase is involved in transcription and was shown to be identical to uracil-DNA glycosylase, a base-excision repair enzyme. We presume that the nuclear functions of these glycolytic enzymes may be thwarted in the XP strains investigated and may account for malfunctions in XP, particularly for neurological disturbances.
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  • 32
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    BIT 34 (1994), S. 177-204 
    ISSN: 1572-9125
    Keywords: 65F10 ; 15A06 ; 65F90 ; 65K10 ; Conjugate gradient method ; preconditioning ; incomplete factorization ; polynomial preconditioner ; matrix-free method ; Fourier analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Notes: Abstract Preconditioning strategies based on incomplete factorizations and polynomial approximations are studied through extensive numerical experiments. We are concerned with the question of the optimal rate of convergence that can be achieved for these classes of preconditioners. Our conclusion is that the well-known Modified Incomplete Cholesky factorization (MIC), cf. e.g., Gustafsson [20], and the polynomial preconditioning based on the Chebyshev polynomials, cf. Johnson, Micchelli and Paul [22], have optimal order of convergence as applied to matrix systems derived by discretization of the Poisson equation. Thus for the discrete two-dimensional Poisson equation withn unknowns,O(n 1/4) andO(n 1/2) seem to be the optimal rates of convergence for the Conjugate Gradient (CG) method using incomplete factorizations and polynomial preconditioners, respectively. The results obtained for polynomial preconditioners are in agreement with the basic theory of CG, which implies that such preconditioners can not lead to improvement of the asymptotic convergence rate. By optimizing the preconditioners with respect to certain criteria, we observe a reduction of the number of CG iterations, but the rates of convergence remain unchanged.
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  • 33
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    Molecular genetics and genomics 243 (1994), S. 253-260 
    ISSN: 1617-4623
    Keywords: Recombinant DNA ; Saccharomyces cerevisiae ; Endo-β-glucanase ; Endo-xylanase ; Heterologous expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have developed a method for fast and efficient isolation of enzyme genes from filamentous fungi by combining the ability of Saccharomyces cerevisiae to express heterologous genes with the utilisation of sensitive and reliable enzyme assays. A cDNA library from the fungus Humicola insolens was constructed in a S. cerevisiae/Escherichia coli shuttle vector in E. coli. Sub-pools of the library were subsequently screened for enzyme activity in S. cerevisiae. More than 130 clones were identified as positive in either an endo-β-glucanase or an endo-xylanase assay. Based on a partial characterization of the DNA sequence of the individual clones, they could be grouped into five distinct types of endo-β-glucanases and three types of endo-xylanases. A representative cDNA from each type was sub-cloned in an Aspergillus vector and expressed in A. oryzae. The new cloning method may be an important alternative to traditional cloning methods based on amino acid sequence information.
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  • 34
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    Journal of High Resolution Chromatography 17 (1994), S. 839-850 
    ISSN: 0935-6304
    Keywords: Capillary GC ; Chemometry ; Fourier analysis ; Multicomponent mixture chromatograms ; PCB analysis ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Aroclor and Aroclor mixture chromatograms obtained using temperature programmed high resolution capillary GC coupled with mass spectrometric selective detection have been investigated by Fourier analysis developed for the study of multicomponent chromatograms. The experimental autocorrelation function (EACF), i.e. peak shape and position correlation, showed a retention - structure pattern which could be associated with both the type of Aroclor and the ion used for selective ion monitoring (m/z value). In particular, o-m and m-m isomerization effects were singled out by EACF analysis. By fitting EACF with previously developed theoretical models, it was possible to characterize a specific Aroclor in terms of the number of PCB congeners present in it. The results obtained agreed closely with the typical Aroclor content. For the Aroclor mixture, the distribution of inter-distance between successive peaks was derived. The two first distribution moments obtained agreed with those derived from retention data reported in the literature. The consequences on the separation performance, i.e. the number of singlets, doublets, etc. in the chromatogram, are discussed.
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  • 35
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    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 10 (1994), S. 659-662 
    ISSN: 0749-503X
    Keywords: Recombinant DNA ; purine salvage enzymes ; conserved sequences ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The nucleotide sequence of a Saccharomyces cerevisiae gene encoding a potential adenine phosphoribosyltransferase (APRT) has been determined. The protein encoded by this gene shows a high degree of similarity with APRTs from a variety of other species. The S. cerevisiae gene, named APT2, has been mapped to chromosome IV. The sequence has been deposited in the GenBank data library under Accession Number L14434.
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  • 36
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    Current genetics 23 (1993), S. 435-442 
    ISSN: 1432-0983
    Keywords: Heat shock ; Recombinant DNA ; Membrane protein ; Nutritional limitation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Using differential hybridization, a gene preferentially expressed during entry into stationary phase has been isolated. Subsequent analysis indicated that this gene corresponds to a heat-shock gene. The nucleotide sequence has been determined. It revealed a 332 aminoacid protein. No similarities to any previously known protein have been noted. The protein is very hydrophobic and is predicted to have a membraneous localisation. In agreement with this hypothesis, the analysis of membrane proteins from stationary-phase cells showed that a strain carrying this gene on a multicopy vector overproduces a protein of 30 kDa. This protein was recognized by antibodies directed against the N-terminal portion of the gene product. Considering its induction in response to heat shock and the apparent molecular weight of its product, this gene was designated HSP30.
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  • 37
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    Current genetics 23 (1993), S. 547-548 
    ISSN: 1432-0983
    Keywords: Autonomously replicating sequence ; Recombinant DNA ; Nested deletions ; Mutagenesis ; Schizosaccharomyces pombe
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Plasmids pSP1 and pSP2 are two new Schizosaccharomyces pombe ars1 multicopy vectors with the Saccharomyces cerevisiae LEU2 and URA3 genes as selectable markers. They are derivatives of S. cerevisiae integrative plasmids. These plasmids allow classical molecular genetic techniques, such as mutagenesis, nested deletions and sequencing, to be performed directly.
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  • 38
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    Journal of cancer research and clinical oncology 119 (1993), S. 675-684 
    ISSN: 1432-1335
    Keywords: Xeroderma pigmentosum fibroblasts ; DNA repair ; Recombinant DNA ; cDNA libraries ; Phage λgt10 ; pBluescript vector in vitro transcripts ; Differential hybridization ; Mitochondrial neuromyopathies ; Genetic defects
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract cDNA libraries constructed from cytoplasmic RNA of normal and xeroderma pigmentosum (XP) fibroblast strains were screened for differential gene expression. XP fibroblast strains included one representative of the complementation groups A, C, D, and one XP variant strain. The XP λgt10 cDNA libraries were differentially screened with in vitro transcripts made from cDNA in the pBluescript vector using both the same XP strain and the normal fibroblast strain. Eight differential clones were detected in the libraries of the XP group A, D, and C strains, which caused stronger signals when probed with transcripts from XP strains than with those from the normal strain. The cDNA clones were sequenced. Seven of the eight clones detected coded for three mitochondrial genes: subunit I of cytochromec oxidase (complex IV of the respiratory chain), apocytochromeb (subunit of complex III), and 16-S rRNA. Two clones representing essentially (a) subunit I of cytochromec oxidase and (b) 16-S rRNA diverged from the sequence of the human mitochondrial genome present in the data-base libraries. Clone a exhibited a transition mutation, clone b reflected a transcript of a mitochondrial genome rearranged in the 16-S rRNA gene, including four nucleotides of the adjacent tRNALeu gene. The apparently enhanced expression of mitochondrial genes in XP cells, together with the changes in DNA sequence, seem to indicate that functions of the ATP-generating system were impaired. This defect may have originated from mutations due to lack of DNA repair. The data can be interpreted in the light of mitochondrial changes that cause human neuromyopathies to occur. In analogy to these diseases the neurological symptoms in XP might be explained by abnormal mitochondria.
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  • 39
    ISSN: 1615-2573
    Keywords: Source pressure ; Fourier analysis ; Contraction duration ; Shortening deactivation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary By fitting isovolumic phases of an ejecting beat with a model-wave function, one can predict source pressure of the ejecting beat (Sunagawa et al.Trans Biomed Eng 1980; 27:299–305), this being a major determinant of systolic performance. Prior applications of this principle have involved two assumptions: (1) that the isovolumic pressure wave is shaped like an inverted cosine wave, and (2) that duration of an isovolumic beat is the same as that of an ejecting beat. The first assumption might cause overestimation of source pressure, since an isovolumic pressure wave begins declining before the midpoint of the wave. The second assumption might cause underestimation of source pressure, since an ejecting beat is always shorter than an adjacent isovolumic beat at the ejecting beat's enddiastolic volume. Although the two errors tend to cancel, it would be more rational and accurate to use a realistic model wave shape and a realistic isovolumic beat duration. To acquire the information necessary for this, pressure and volume time courses were measured during ejecting beats and adjacent isovolumic beats in dogs under the following steady-state conditions: basal, atrial pacing at various rates, infusion of dobutamine, infusion of verapamil, coronary ligation(s), and ventricular pacing at various sites. These conditions affected the amplitude and duration of isovolumic pressure waves substantially but did not affect the shape of the waves significantly. The duration of each isovolumic beat exceeded that of the previous ejecting beat to a degree which corresponded approximately to the ejecting beat's normalized pressure reserve (source pressure minus peak ejection pressure)/(source pressure). A more accurate source-pressure prediction should be possible by use of a realistic isovolumic pressure-wave shape and by taking account of the effect of pressure reserve on contraction duration.
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  • 40
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    Annals of the Institute of Statistical Mathematics 44 (1992), S. 13-25 
    ISSN: 1572-9052
    Keywords: Fourier analysis ; array estimation ; velocity estimation ; space-time spectral analysis ; random media ; random arrays ; frequency wavenumber processing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Notes: Abstract Consider the situation where a plane wave signal is received by a spatial arrangement of recorders. Information derived from observations on such a process can be used to determine the speed and direction of the signal together with properties of the medium through which the signal is being propagated. Certain models for the case where the signal velocity can be regarded as stochastic and where the array is irregular are investigated and estimation procedures proposed. A major practical property of these models is that, unlike their deterministic counterparts, coherence decays to zero as distance between recorders increases.
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  • 41
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    Journal of industrial microbiology and biotechnology 10 (1992), S. 185-190 
    ISSN: 1476-5535
    Keywords: β-Glucosidase ; C. flavigena ; Cellobiase ; Recombinant DNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Plasmid-coded β-glucosidase produced byEscherichia coli was characterized and compared to the enzyme produced byCellulomonas flavigena. Cell-free extracts, non-denaturing PAGE and 5-bromo-4-chloro-3-indolyl-β-d-glucopyranoside (X-glu) as substrate were used to compare both enzymes. The β-glucosidase was assayed for cellobiose andp-nitrophenyl-glucopyranoside (PNPG). Cellobiose hydrolysis was performed at 50°C for the enzyme fromC. flavigena and at 37°C for that fromE. coli pJS3, both with an optimal pH of 6.5. For PNPG hydrolysis, the optimal conditions were pH 5.5 and 37°C for both cell extracts. Most of the β-glucosidase activity was intracellular. When cultures ofC. flavigena were grown with cellobiose or carboxymethylcellulose (CMC) as inducers, the expression of β-glucosidase was increased considerably.E. coli pJS3 produces a cellobiase which hydrolyzes cellobiose and PNPG. TheK m values for cellobiose and PNPG indicated that the β-glucosidase activity ofC. flavigena had a higher affinity for cellobiose as substrate, whereas the β-glucosidase fromE. coli pJS3 showed higher affinity for PNPG.
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  • 42
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    Hydrobiologia 247 (1992), S. 109-117 
    ISSN: 1573-5117
    Keywords: Crassostrea cucullata ; Fourier analysis ; shell form ; shell length ; regression analysis ; environmental factors
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Variations of shell form and shell length were studied for oysters growing in the mangroves of Gazi Creek, Kenya, and related to different environmental factors. For the study of the form, Fourier analysis was performed on the circumference of 85 oysters. The resulting coefficients were compared among specimens using cluster analysis. The correspondence between this classification and substrate diameter is virtually perfect (only one misclassification out of 85 oysters). For the clusters based on height above chart datum, 9 specimens, all on intermediate height levels, were misclassified. Orientation with respect to tidal current had 13 misclassifications. Mangrove species seemed to influence form only marginally, if at all. In the study of the size ofCrassostrea cucullata, the length of 956 oysters, growing along two transects were measured, and correlated with several environmental factors. Oyster length was not related to substrate diameter or its orientation with respect to the main current. Length was not influenced by density up to a cover of 70%. For densities higher than 70%, there was a fairly strong negative correlation (r 2 = 0.634,n = 217). Length was not correlated with height above bottom (base of the tree) for heights lower than 20 cm, while oysters growing closer to the bottom were smaller. The correlation with height above chart datum was negative but very low (r 2 = 0.060,n = 957). However, if all measurements of oysters closer than 20 cm to the bottom, and all from a density of more than 70% cover are deleted from the data set, the correlation with height increased dramatically, the slope still being negative (r 2 = 0.859,n = 543).
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  • 43
    ISSN: 1619-7089
    Keywords: Technetium-99m methoxyisobutylisonitrile single photon emission tomography (SPET) ; Amplitude images ; Fourier analysis ; Thallium-201 SPET
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract To determine the role of rest and stress gated technetium-99m methoxyisobutylisonitrile (sestamibi), in the detection of coronary artery disease, routine Fourier analysis of these images was performed with the best septal left anterior oblique (LAO) position of 20 patients (17 men, 3 women; aged 40–75 years) who also underwent rest or redistribution/stress single photon emission tomography (SPET) (99mTc-sestamibi and Thallium-201), gated blood pool imaging and coronary angiogram. There were 6 patients with single-vessel disease, 6 with two-vessel disease, 4 with three-vessel disease, 2 with coronary spasms, 1 with a patent graft and 1 with anginal episodes but a normal angiogram result. Three normal volunteers (2 women, 1 man; aged 24–26 years) also had rest and stress gated blood pool as well as rest and stress gated 99mTc-sestamibi imaging. Rest and stress 99mTc-ses-tamibi amplitude and phase images depicted regional myocardial wall shortening from the outer layer of the myocardium to the center of the left ventricle as follows a high amplitude halo of maximal negative count rate variaton; a circular thinner halo of negligible amplitude; a central region of maximal positive count rate variation, as the images evolved from end-diastole to end-systole. Similar patterns with regional differences represented abnormal myocardial wall shortening. (99mTc-sestamibi and 201T1 SPET) images were in agreement in 90% of the patients and 92% of myocardial regions. 201T1 SPET detected 83% of angiographically proven lesions, as compared with 80% for 99mTc-setamibi SPET and 80% for the amplitude images. The amplitude images demonstrated a larger number of other abnormalities not predicted on the angiogram, probably because they were able to detect regions with a potential for flow improvement and transient regional wall shortening abnormalities. Amplitude and phase analyses of gated rest and stress 99mTc-ses-tamibi images are easy to perform and may become an important adjunct to (99mTc-sestamibi SPET) images for a complete evaluation of both regional myocardial perfusion and regional contractile function using a single tracer.
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  • 44
    ISSN: 1617-4623
    Keywords: Recombinant DNA ; Shotgun cloning ; Filamentous fungi ; Promoter sequence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A transformation system for the thermophilic cellulolytic fungus Talaromyces sp. CL240 has been developed, using the phleomycin resistance gene from Streptoalloteichus hindustanus (Sh ble) as a dominant selectable marker. The plasmids (pAN8-1 and pUT720) carrying the Sh ble gene under the control of the Aspergillus nidulans glyceraldehyde-3-phosphate dehydrogenase (gpd) promoter, allowed selection of phleomycin-resistant transformants. A new promoter sequence cloned from chromosomal DNA of Trichoderma reesei (pUT737) was also able to drive efficient expression of the Sh ble gene in Talaromyces sp. CL240, resulting in the selection of transformants that were highly resistant to phleomycin.
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  • 45
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    Molecular Reproduction and Development 31 (1992), S. 161-169 
    ISSN: 1040-452X
    Keywords: Sperm cell ; Recombinant DNA ; Fertilization ; Genetic transformation ; Transgenic animals ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Epididymal sperm cells, incubated with plasmid DNA, showed a spontaneous tendency to interact with the exogenous nucleic acid. We have investigated the molecular basis of such interaction. Exogenous DNA is taken up by sperm cells over a 15- to 20-min period and is specifically localized on the nuclear area of the sperm head. DNA was reversibly bound to spermatozoa since it can be competed out by excess of cold competitor DNA or by other polyanions as heparin and dextran sulphate. By contrast, poly-L-lysine, a polycation, favours the uptake. DNA molecules of large size (7 kb) were preferentially taken up as compared to smaller ones (150-750 bp). Acidic proteins were also taken up and concentrated, as for DNA, at the nuclear level. These data strongly suggested that ionic interactions may occur between foreign molecules and a substrate located in the sperm head. On the basis of Southwestern analysis, a sperm head protein(s) of 30-35 KD is identified as potential substrate for exogenous DNA binding. Moreover, we have found that seminal plasma contains factor(s) which abolish sperm permeability, exerting a powerful inhibitor effect on DNA uptake. The presence of a specific binding protein for the DNA and of a factor inhibiting such interaction support the existence of a mechanism controlling, through specific factors, the sperm-DNA interaction.
    Additional Material: 9 Ill.
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  • 46
    ISSN: 1573-6792
    Keywords: Obsessive-compulsive disorder ; EEG ; Theta rhythm ; Fourier analysis ; Coefficient of variations
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Spectral EEG characteristics of thirteen patients with severe Obsessive-Compulsive Disorder (OCD) were investigated topographically. The finding of predominantly left posterior frontal to mid-temporal theta-2 is discussed in light of previous EEG studies and recent neuroradiologic findings.
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  • 47
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    Brain topography 4 (1992), S. 309-319 
    ISSN: 1573-6792
    Keywords: Principal components ; Isotropicity ; Complex statistics ; Electroencephalography ; Equivalent dipoles ; Fourier analysis ; Brain mapping
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The structure of the normal resting EEG crosspectrum Svv(ω) is analyzed using complex multivariate statistics. Exploratory data analysis with Principal Component Analysis (PCA) is followed by hypothesis testing and computer simulations related to possible neural generators. The Svv(ω) of 211 normal individuals (ages 5 to 97) may be decomposed into two types of processes: the ξ process with spatial isotropicity reflecting diffuse, correlated cortical generators with radial symmetry, and processes that seem to be generated by more spatially concentrated, correlated sources. The latter are reflected as spectral peaks such as the process. The eigenvectors of the ξ process are the Spherical Harmonic Functions which explains the recurring pattern of maps characteristic of the spatial PCA of qEEG data. A new method for estimating sources in the frequency domain which fits dipoles to the whole crosspectrum is applied to explain the characteristics of the localized sources.
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  • 48
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    Molecular genetics and genomics 232 (1992), S. 498-504 
    ISSN: 1617-4623
    Keywords: luminescence ; Recombinant DNA ; Grampositive organisms ; Shuttle vectors ; luc and lux genes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A group of vectors for luciferase expression in Bacillus subtilis was constructed. So far, only bacterial luciferases have been expressed in Bacillus, but in this study we wanted also to express genes encoding eukaryotic luciferases to perform direct comparisons of the light levels produced by the two different systems in B. subtilis. The vectors constructed can replicate both in Escherichia coli and B. subtilis, and the luciferase expression is strictly regulated due to the dual plasmid system used. Nearly a 100-fold increase in light production compared to previous results was achieved when genes encoding bacterial luciferase were inserted into the constructs and transformed into B. subtilis. An additional tenfold increase in light production was obtained when luciferase genes from the North American firefly (Photinus pyralis) or a click beetle (Pyrophorus plagiophtalamus) were introduced in a similar fashion into B. subtilis. Measurement of the light emission was performed without disruption of bacterial cells in a real-time manner, which is a common feature when working with all of these constructions. Structures of the shuttle vector constructs and results from light emission measurements are presented.
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  • 49
    ISSN: 1617-4623
    Keywords: Secretion ; Recombinant DNA ; Hemolysin ; HlyB/H1yD complementation ; OmpT protease
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A fusion gene (ces-hlyA s) was constructed by ligating the genetic information for the C-terminal 60 amino acids (hlyA s) ofEscherichia coli hemolysin (H1yA) to the ces gene for a cholesterol esterase/lipase (CE) from aPseudomonas species. Part (about 30 %) of the expressed fusion protein CE-H1yAs was secreted inE. coli carryinghlyB andhlyD genes. Following the insertion between the reporter gene andhlyA s of a linker sequence that contains the information for potential cleavage sites for the outer membrane protease OmpT, two different fusion proteins (PhoA-H1yAs and CE-HlyAs) were shown to be cleaved by OmpT between the two parts during H1yB/H1yD-mediated secretion. Processed PhoA and CE accumulated in the supernatant. The efficiency of cleavage by OmpT was considerably improved by increasedompT gene dose. It was further shown that OmpT preferentially recognizes potential cleavage sites within the linker sequence.
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  • 50
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    European archives of psychiatry and clinical neuroscience 240 (1991), S. 197-203 
    ISSN: 1433-8491
    Keywords: Schizophrenia ; Recombinant DNA ; Genetic markers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Interest in genetic marker studies of schizophrenia has been considerably enhanced by the advent of recombinant DNA technology, which has dramatically increased the number of available markers. In the present paper, we review studies that have been carried out using classical markers as well as the more recent molecular studies. The problems that arise when schizophrenia is studied in this way are discussed and attempts are made to account for some of the conflicting findings in this area.
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  • 51
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    European journal of epidemiology 7 (1991), S. 213-221 
    ISSN: 1573-7284
    Keywords: Rickettsiae ; Rickettsial genetics ; Genes ; Recombinant DNA ; Cloning ; Electroporation ; Transformation Rickettsia ; Coxiella ; Rochalimaea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Classical genetic approaches useful with free-living bacteria are difficult to apply to the rickettsiae. Although rickettsial mutants have been isolated over the years, the genetic basis of these mutants is unknown, limiting their usefulness. The application of molecular biological techniques to rickettsial studies has provided the opportunity to isolate and study specific genes. Genes encoding metabolic enzymes from rickettsiae were cloned in Escherichia coli and shown to retain their regulatory properties, suggesting that recombinant DNA technology may be useful for studies of rickettsial enzymes and regulatory mechanisms. The potential use of rickettsial surface components, or virulence factors as possible antigens for protective subunit vaccines, has led to the cloning and expression in E. coli, of rickettsial chromosomal and plasmid genes encoding outer membrane proteins. The number of genes characterized in recent years has increased dramatically giving rise to an increasing source of information on rickettsial gene structure. Plasmids have only been identified in C. burnetii and possibly Rochalimaea quintana. The plasmid sequences present in C. burnetii are highly conserved suggesting that they are important to the growth and virulence of this organism. To understand the role of genes in the rickettsia-host relationship, it is critical that a genetic exchange system be developed. The recent description of transformation of R. quintana by electroporation is an important first step in this direction. The ability to introduce genetic material is necessary to address questions that cannot be resolved by studying rickettsial gene expression in E. coli.
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  • 52
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    European journal of applied physiology 63 (1991), S. 300-307 
    ISSN: 1439-6327
    Keywords: Oxygen uptake ; Carbon dioxide output ; Ventilation ; Pseudorandom binary sequence ; Fourier analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The dynamics of ventilation (VE), oxygen uptake (VO2), carbon dioxide output (VCO2), and heart rate (f c) were studied in 12 healthy young men during upright and supine exercise. Responses to maximal and to two different types of submaximal exercise tests were contrasted. During incremental exercise to exhaustion, the maximal work rate, VO2max, VEmax, f c,max, and ventilatory threshold were all significantly reduced in supine compared to upright exercise (P〈0.01–0.001). Following step increases or decreases in work rate between 25 W and 105 W, both VO2 and VCO2 responded more slowly in supine than upright exericse. Dynamics were also studied in two different pseudorandom binary-sequence (PRBS) exercise tests, with the work rate varying between 25 W and 105 W with either 5-s or 30-s durations of each PRBS unit. In both of these tests, there were no differences caused by body position in the amplitude or phase shifts obtained from Fourier analysis for any observed variable. These data show that the body position alters the dynamic response to the more traditional step increase in work rate, but not during PRBS exercise. It is speculated that the elevation of cardiac output observed with supine exercise in combination with the continuously varying work-rate pattern of the PRBS exercise allowed adequate, perhaps near steady-state, perfusion of the working muscles in these tests, whereas at the onset of a step increase in work rate, greater demands were placed on the mechanisms of blood flow redistribution.
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  • 53
    ISSN: 1617-4623
    Keywords: Recombinant DNA ; Gene cloning ; Ubiquitin fusion gene ; Ubiquitin extension protein ; Expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The genome of Tetrahymena pyriformis has been shown to contain a ubiquitin multigene family consisting of several polyubiquitin genes and at least one ubiquitin fusion gene. We report here the isolation and characterization of one genomic clone (pTUl1), that encodes a ubiquitin extension protein. A comparison of the predicted amino acid sequence of the ubiquitin extension protein gene of T. pyriformis with those from other organisms indicated a high degree of homology. However, the Tetrahymena ubiquitin extension protein contains 53 and not 52 amino acids. This feature is different from all ubiquitin 52-amino-acid extension protein genes thus far sequenced. Furthermore, we found an array of four cysteine residues similar to those found in nucleic acid binding proteins. Also, the C-terminal sequence possesses a conserved motif which may represent a nuclear translocation signal. The ubiquitin 53-amino-acid extension protein gene encodes the smallest class of ubiquitin mRNAs in T. pyriformis.
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  • 54
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    Molecular genetics and genomics 227 (1991), S. 28-32 
    ISSN: 1617-4623
    Keywords: Recombinant DNA ; Antibiotic production ; Genetic organization ; Directed mutant screen ; Prodigiosin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Fifteen mutants ofStreptomyces coelicolor A3(2) blocked in both the bipyrrole branch (redA) and a second site specific to the undecylprodigiosin pathway were characterized. Some of the mutants were ordered biosynthetically based on cosynthesis experiments. Complementation of each of the mutants with wild-type DNA cloned in low- and high-copy number plasmid vectors allowed the mutants to be separated into 12 new classes which are physically clustered within approximately 37 kb on theS. coelicolor genome. Early-step biosynthetic genes are centrally located and are flanked by later-step and regulatory genes.
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  • 55
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    International Journal for Numerical Methods in Fluids 13 (1991), S. 579-597 
    ISSN: 0271-2091
    Keywords: Diffusion-convection ; Fourier analysis ; Stability ; Artificial viscosity ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: This paper develops a stability analysis of second-order, two- and three-time-level difference schemes for the 2D linear diffusion-convection model problem. The corresponding 1D schemes have been extensively analysed in two previous papers by the same author. Most of these 2D schemes obviously generalize 1D schemes, i.e. their stencil only uses the nearest points and defines ‘product difference schemes’; however, the stability results are not always the exact generalization of the 1D stability properties. Moreover, the 1D nonviscous MFTCS scheme may only be generalized if one uses a nine-point scheme. Numerical experiments for different values of the cell Reynolds number allow a comparison to be made between the theoretical and numerical stability limits.
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  • 56
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    Current genetics 17 (1990), S. 223-227 
    ISSN: 1432-0983
    Keywords: Orotidine-5′-phosphate decarboxylase ; Cephalosporium acremonium ; Recombinant DNA ; Evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We have cloned the Cephalosporium acremonium pyr4 gene by cross-hybridization with the equivalent gene from Neurospora crassa, the closest relative from which this gene is available. The C. acremonium pyr4 gene complements an E. coli pyrF mutant lacking orotidine-5′-phosphate decarboxylase (OMPdecase), and most probably does not contain introns. Maxicell analysis in E. coli shows that it encodes a 46 kDa polypeptide. The C. acremonium OMPdecase contains a highly conserved pentadecapeptide characteristic for this category of enzyme. Extensive sequence comparison suggests an important role of this region in enzymatic activity.
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  • 57
    ISSN: 1476-5535
    Keywords: Fermentation ; Recombinant DNA ; Phage λp L promoter ; Expression vector ; α1-Antitrypsin ; Malaria vaccine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The major leftward early promoter of phage λp L, has frequently been used to drive expression of heterologous genes inEscherichia coli.p L is typically maintained fully repressed by the lambda cl protein. When induction of heterologous protein synthesis is desired, one of several potential mechanisms of destroying cl function is employed and the expression of the foreign gene commences. One method of derepressingp L involves exposing cells to nalidixic acid, which results in the “activation” of RecA protein and the subsequent RecA-mediated proteolytic cleavage of cl. Activated RecA also mediates the cleavage of theE. coli LexA protein, resulting in induction of the SOS regulon (at least 15E. coli genes, includingrec A). We have examined the effect of two chromosomal mutations on the productivity of nalidixic acid inductions. One of the tested mutations (recA o) increased the intracellular concentration of RecA prior to induction; the other (lexAind−) resulted in a mutated lexA protein insensitive to RecA-mediated cleavage. These mutations were introduced into a strain carrying acl+ defective lysogen. Synthesis of two heterologous proteins, human α1-antitrypsin and a fusion protein partially derived from thePlasmodium falciparum circumsporozooite surface antigen, was examined in the wild-type and mutant strains. The maximum α-1 antitrypsin concentration achieved was improved by 50% when therecA o strain was used rather than the wild type; however; only smaller changes (20% or less) in the maximum concentration of the malaria fusion protein wer observed. Use of thelexAind− strain resulted in a decrease in the maximum concentration attained for both heterologous products.
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  • 58
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    Documenta ophthalmologica 76 (1990), S. 65-71 
    ISSN: 1573-2622
    Keywords: Coefficient of variation ; domain of frequency ; domain of time ; Fourier analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The dark-adapted and light-adaped electroretinograms of 13 subjects with 23 normal eyes were analyzed by means of Fourier spectrum. The oscillatory potentials in the time domain were filtered out from the electroretinogram after a corresponding bandpass was given in the frequency domain. The coefficient of variation of total power, dominant power and dominant frequency of the isolated oscillatory potentials in the frequency domain, summed amplitudes and area of the isolated oscillatory potentials, each amplitude and implicit time of the first four major oscillatory potential wavelets in the time domain were compared. The implicit time showed the smallest coefficient of variation; summed amplitudes of OP1 to OP4 showed smaller coefficients of variation than those of the area, the amplitude of each oscillatory potential wavelet, dominant frequency and dominant and total power. The coefficient of variation of these measurement parameters in light-adapted electroretinograms was smaller than those in dark-adapted electroretinograms.
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  • 59
    ISSN: 1741-0444
    Keywords: Aortic regurgitation ; Arterial compliance ; Fourier analysis ; Impedance ; Mean aortic pressure ; Peripheral resistance ; Windkessel model
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract Three methods for measuring arterial compliance when aortic regurgitation is present are examined. The first two methods are based on a Windkessel model composed of two elements, compliance C and resistance R. Arterial compliance was estimated from diastolic pressure waveforms and diastolic regurgitant flow for one method, and from systolic aortic pressure waveforms and systolic flow for the other method. The third method was based on a three-element Windkessel model, composed of characteristic resistance r, compliance C and resistance R. In this method arterial compliance was calculated by adjusting the model to the modulus and phase of the first harmonic term of the aortic input impedance. The three methods were compared and validated in six anaesthetised pigs over a broad range of aortic pressures. The three methods were found to give quantitatively similar estimates of arterial compliance at mean aortic pressures above 60 mm Hg. Below 60 mm Hg, estimates of arterial compliance varied widely, probably because of poor validity of the Windkessel models in the low pressure range.
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  • 60
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    Molecular genetics and genomics 222 (1990), S. 249-256 
    ISSN: 1617-4623
    Keywords: Recombinant DNA ; Plant virus ; T7 promoter ; In vitro transcription
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Full-length cDNA copies of cucumber mosaic virus (CMV) RNAs 1 and 2 of the Fny strain were constructed from partial cDNA clones and were cloned downstream of bacteriophage T7 promoters. In one pair of clones, transcription proceeded from an unaltered T7 promoter such that in vitro transcripts representing RNAs 1 and 2 contained an additional 17 nucleotides at their 5′ termini. In a second pair of clones, the T7 promoter/cDNA junction was altered by oligonucleotide-directed mutagenesis such that the in vitro transcripts contained only an additional G residue at their 5′ ends. In addition, a full-length cDNA copy of Fny-CMV RNA 3 was constructed from two overlapping cDNA clones and was cloned downstream of an altered T7 promoter such that the resultant in vitro transcripts also contained only an additional G residue at their 5′ ends. In vitro transcripts derived from all clones contained an additional C residue at their 3′ ends. In vitro transcripts representing RNAs 1, 2 and 3 which contained an additional residue at each terminus were shown to be infectious together in several hosts of CMV.
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  • 61
    ISSN: 1617-4623
    Keywords: Recombinant DNA ; Vaccine development ; Carrier protein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Hypervariable regions (HRs) of the major subunit of F11 fimbriae were exploited for insertion of foreign epitopes. Two insertion vectors were created that contain a unique cloning site in HR1 or HR4 respectively. Several oligonucleotides, coding for antigenic determinants derived from different pathogens, were cloned in both insertion vectors. Hybrid fimbrial subunits were generally shown to be assembled in fimbriae when the length of the inserted peptide did not exceed 14 amino acids. The inserted peptides appeared to be exposed in the fimbrial filament. One hybrid fimbrial protein induced detectable levels of antibodies against the inserted epitope if injected into mice.
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  • 62
    ISSN: 1617-4623
    Keywords: Monooxygenase ; Recombinant DNA ; Overexpression ; Upstream reading frames ; Complementation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The gene coding for benzoate-para-hydroxylase (bphA) of Aspergillus niger was cloned using differential hybridisation techniques and complementation of mutants deficient in this enzyme activity. The nucleotide sequence of the gene was determined, the presence of two introns was shown and the transcription start and termination sites were determined. The structure of the mRNA upstream from the long open reading frame (ORF) is unusual. It contains two small, overlapping ORFs whose function is unknown. Comparison of the deduced amino acid sequence of the protein with the sequences present in the databanks, indicated a significant similarity of BPH to the superfamily of cytochrome P450 enzymes. Further analysis revealed that this protein is a member of a new P450 gene family designated P450LIII. The gene is designated CYP53. To increase the BPH activity of A. niger, multiple copies of the bphA gene were introduced into the genome of a recipient strain by transformation. Although increased intracellular levels of the BPH protein could be detected, the BPH enzyme activity was decreased, suggesting titration of another essential component.
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    International Journal for Numerical Methods in Fluids 11 (1990), S. 427-444 
    ISSN: 0271-2091
    Keywords: Fourier analysis ; Eulerian-Lagrangian ; Least squares ; Transport ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: A Fourier analysis was performed in order to study the numerical characteristics of the effective Eulerian-Lagrangian least squares collocation (ELLESCO) method. As applied to the transport equation, ELLESCO requires a C1-continuous trial space and has two degrees of freedom per node. Two coupled discrete equations are generated for a typical interior node for a one-dimensional problem. Each degree of freedom is expanded separately in a Fourier series and is substituted into the discrete equations to form a homogeneous matrix equation. The required singularity of the system matrix leads to a ‘physical’ amplification factor that characterizes the numerical propagation of the initial conditions and a ‘computational’ one that can affect stability.Unconditional stability for time-stepping weights greater than or equal to 0-5 is demonstrated. With advection only, ELLESCO accurately propagates spatial wavelengths down to 2Δx. As the dimensionless dispersion number becomes large, implicit formulations accurately propagate the phase, but the higher-wave-number components are underdamped. At large dispersion numbers, phase errors combined with underdamping cause oscillations in Crank-Nicolson solutions. These effects lead to limits on the temporal discretization when dispersion is present. Increases in the number of collocation points per element improve the spectral behaviour of ELLESCO.
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    Pure and applied geophysics 131 (1989), S. 211-239 
    ISSN: 1420-9136
    Keywords: Fourier analysis ; fractal ; frequency spectra ; roughness ; stationarity ; numerical modeling
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract Fractal analysis and Fourier analysis are independent techniques for quantitatively describing the variability of natural figures. Both methods have been applied to a variety of natural phenomena. Previous analytical work has formulated relationships between the fractal dimension and power law form frequency spectrum.Mandelbrot (1985) has shown that difficulties arise when the ruler method for measuring dimensionality is applied to other than self-similar figures. Since an investigator presumably does not know in advance the dimensionality of a natural profile, it is essential to quantify the nature of the discrepancy for self-affine cases. In this study, a series of experiments are conducted in which discrete random series of specified spectral forms are analyzed using the fractal ruler method. The various parameters of the fractal measurement are related to the parameters of the spectral model. In this way, empirical relationships between the techniques can be derived for discrete, finite series which simulate the results of applying the fractal method to observational data. The results of the study indicate that there are considerable discrepancies between the results predicted by theory and those derived empirically. The fundamental power law form of length versus resolution pairs does not hold over the entire region of analysis. The predicted linear relationship between fractal dimension and exponent of the frequency spectrum does not hold, and the spectral signals can be extended beyond the limits of dimension inferred by theory. Root-mean-square variability is also shown to be linearly related to the fractal intercept term. An investigation of the effect of nonstationary sampling is conducted by generating signals composed of segments of differing spectral characteristics. Fractal analyses of these signals appear identical to those conducted on stationary series. The discrepancies between theoretical prediction and empirical results described in this study reflect the difficulties of applying analytically derived techniques to measurement data. Both Fourier and fractal techniques are formulated through rigorous mathematics, assuming various conditions for the underlying signal. When these techniques are applied to discrete, finite length, nonstationary series, certain statistical transformations must be applied to the data. Methods such as windowing, prewhitening, and anti-aliasing filters have been developed over many years for use with Fourier analysis. At present, no such statistical theory exists for use with fractal analysis. It is apparent from the results of this study that such a statistical foundation is required before the fractal ruler method can be routinely applied to observational data.
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    Computers and the humanities 23 (1989), S. 277-283 
    ISSN: 1572-8412
    Keywords: Statistics ; correlation analysis ; principal components analysis ; time series analysis ; Fourier analysis ; semantic patterning ; imagery
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science , Media Resources and Communication Sciences, Journalism
    Notes: Abstract This paper describes a computer-assisted analysis of semantic patterning in William Blake'sThe Four Zoas and considers the way in which such patterns contribute to the structure and meaning of the work. The analysis involves examining combinations and recombinations of images across the text for concentrations of images and images groups, recurring images, and patterns in the distribution of individual images and clusters of images. Statistical correlation routines were used to determine the degree of correlation among images across the extire text as well as in specific text segments. Principal components analysis enabled identifying thematic clusters of images, and the distribution of these clusters across these text were in turn examined to determine their patterning. Finally, time series analysis and Fourier analysis were used to find and verify patterns in the distribution of images across the text. Fourier analysis revealed striking patterns in the distribution of imagery in theZoas, which suggests that Blake may have used such patterns to help convey the poem's powerful thematic statements.
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    Documenta ophthalmologica 73 (1989), S. 347-357 
    ISSN: 1573-2622
    Keywords: pattern electroretinogram ; Fourier analysis ; fovea ; retinal eccentricity ; spatial tuning ; visual field
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract To investigate the value of the extrafoveal pattern electroretinogram for evaluation of local retinal defects, electroretinographic responses to contrast reversal stimulation were recorded at various locations in the central and midperipheral retina. Normal values were established in 20 eyes, and spatial selectivity at different retinal eccentricities was determined in four eyes. The response amplitude was found to decrease steeply from the fovea until about 12° eccentricity and to decline only slowly thereafter. For central stimulation a clear attenuation for coarser patterns was observed. At 20° and at 30° eccentricity the spatial tuning function exhibited a bandpass characteristic, with the maximum amplitude shifting to lower spatial frequencies. Despite the relatively low peripheral amplitudes a satisfactory signal to noise ratio of the second harmonic responses can be obtained by Fourier analysis, which improves clinical applicability. The comparison of sensitivity in the upper and lower retina revealed a considerable asymmetry, with responses in the upper retina approximately 20% higher. No significant difference, however, was found when the temporal or nasal retina was stimulated. The reproducibility of these results is high enough to encourage clinical studies. A case of retinal venous branch occlusion exemplifies the applicability of this technique in eccentric fundus lesions.
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  • 67
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    Documenta ophthalmologica 72 (1989), S. 9-19 
    ISSN: 1573-2622
    Keywords: binocular facilitation ; binocular summation ; flicker ; Fourier analysis ; pattern reversal ; visual evoked potentials
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Evidence is controversial in respect to the optimal conditions in which visual evoked potentials provide an objective measure of binocular visual function, related and unrelated to stereopsis, and there is little emphasis on the type of stimulus that produces facilitation in binocular recording. We investigated the effects of stimulus type (flicker or pattern), contrast, and temporal modulation on facilitation, which was defined as a binocular response greater than sum of monocular responses. Monocular and binocular responses to sinusoidally modulated flicker and grating patterns were recorded in children and Fourier analyzed. The relationship of the fundamental Fourier component for flicker and the second harmonic component for pattern were each examined as function of temporal modulation at two levels of contrast for monocular and binocular visual evoked potentials. Binocular facilitation was found across all conditions for flicker. Data suggest that processing of pattern and flicker has different sites of origin within the visual system. Facilitation in binocular visual evoked potentials also indicates that they are not a result of simple summation of monocular responses, since there appears a nonlinear component to such interaction.
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  • 68
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    Water resources management 3 (1989), S. 259-269 
    ISSN: 1573-1650
    Keywords: Evapotranspiration ; stochastic model ; Fourier analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Architecture, Civil Engineering, Surveying , Geography
    Notes: Abstract Daily meteorological data over a period of nine years from Helliniko Station (Athens, Greece) are used to produce daily potential evapotranspiration (PET) series. A multipliative model describes the periodic and stochastic components of the series where Fourier aproximations of two- or three-harmonic periodic components and a first-order autoregressive one are employed, respectively. The assessment of adequacy of the model is based on the properties of (a) the correlogram and (b) the spectrum of a white noise process. The model is further used to generate daily PET values. Generated and historical data show remarkable affinity in average arithmetic mean, coefficient of variation, skewness coefficient and their standard deviations.
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  • 69
    ISSN: 1617-4623
    Keywords: metC ; Cystathionine-β-lyase ; Nucleotide sequence ; Recombinant DNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The DNA sequence of the Salmonella typhimurium metC gene and its flanking regions was determined. The metC gene contains an open reading frame of 1185 nucleotides encoding a polypeptide of 395 amino acids with a predicted molecular weight of 42874 daltons. S1 nuclease mapping experiments located the transcription start site of the metC gene. The nucleotide sequence and the deduced amino acid sequence for the metC genes of S. typhimurium and Escherichia coli were compared. Although there are 279 nucleotide replacements, most do not change the amino acid sequence. Nucleotide sequence analysis of the flanking regions of the S. typhimurium metC gene shows that there is an open reading frame upstream and an open reading frame downstream of the gene. The existence of the divergently transcribed upstream open reading frame (designated ORF1) was confirmed by the construction of an ORF1-lacZ fusion. The transcription start site of ORF1 was determined by S1 nuclease mapping.
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  • 70
    ISSN: 1619-7089
    Keywords: Ischemic heart disease ; Gated radionuclide ventriculography ; Regional left ventricular motility injury ; Fourier analysis ; Factor analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Left ventricular phase and amphlitude images (Fourier analysis, PAI) and factor analysis images (FAI) from gated radionuclide ventriculography were obtained in 235 patients after myocardial infarction (MI) and in 44 patients with well documented ischemic heart disease (IHD) in order to assess areas of regional left ventricular motility injury (LVMI). The sensitivity of FAI for LVMI detection was higher than with PAI (36.3% vs 22.7% in patients without MI; 76.6% vs 68% in those after anterior MI; and 53.2% vs 31.9% after posterior MI, respectively). In 2.9% of all patients PAI were unclear due to small time activity amplitudes and heart rate irregularity, whereas FAI could be easily assessed. Significantly decreased left ventricular ejection fraction was observed predominantly after anterior MI in connection with distinct signs of LVMI in a large area of anterior wall or in the anteroseptal and/or apical region. Areas of LVMI could be sharply delineated in FAI; however, in contrast to PAI, FAI is unable to distinguish between dyskinetic and akinetic regions. The use of both PAI and FAI is recommended for more detailed detection of regional LVMI in patients with IHD.
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  • 71
    ISSN: 1617-4623
    Keywords: Recombinant DNA ; Small subunit ; Ribosomal DNA ; Sequence comparison ; Lycopersicon esculentum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The gene of a cytoplasmic 18 S ribosomal RNA (18 S rDNA) of the dicotyledonous plant tomato (ycopersicon esculentum) cv. Rentita has been cloned, and its complete primary structure has been determined. The tomato 18 S rDNA is 1805 by long with a G+C content of 49.6%. Its sequence exhibits 94%–96% positional identity when it is colinearly aligned with the previously reported sequences of the 17–18 S rDNAs of the dicot soybean and the monocots maize and rice. A model of the secondary structure of the 18 S rRNA of angiosperms is presented and its genera-specific structural features are compared with a current eukaryotic 18 S rRNA consensus model.
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  • 72
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    New York : Wiley-Blackwell
    Journal of Bioluminescence and Chemiluminescence 4 (1989), S. 551-554 
    ISSN: 0884-3996
    Keywords: Recombinant DNA ; luminescence ; luciferase gene ; Vibrio harveyi ; toxic substances ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: We have studied possibilities for constructing Escherichia coli strains capable of producing stable light. Light production in E. coli is achieved by cloning the genes encoding bacterial luciferase from Vibrio harveyi. To gain the advantage of sensitive detection of light we transferred the genes under the control of a strong, regulatable promoter system.Stabilization of light produced by E. coli clones was accomplished by finding the optimal plasmid construction and growth conditions as well as suitable measuring buffers. The adjustment of the luciferase synthesis for bioluminescence measurements to a high but not harmful level gives healthy cells and stable luciferase. Cultivation at 30 °C in an uninduced state was found to be the most important factor in getting stable-light production. The overall cell metabolism being unstressed gives us the possibility of monitoring cell physiology and factors affecting it via bioluminescence reactions in vivo. To make the results easy to interpret the light emission has to be stable during a measurement period of one to several hours. In the case of the original light-producing bacteria, Vibrio and Photobacterium strains it has not thus far been possible to find conditions where light emission would be stable for several hours. Based on our findings an automated biosensor system can be developed to monitor the effects of biologically active compounds against stable-light-producing bacteria.
    Additional Material: 1 Ill.
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  • 73
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    Chichester : Wiley-Blackwell
    International Journal for Numerical Methods in Fluids 9 (1989), S. 811-832 
    ISSN: 0271-2091
    Keywords: Fourier analysis ; Non-uniform mesh ; Wave reflection/transmission ; Group velocity ; Crank-Nicolson finite elements ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Internal wave reflections and transmissions are examined for the Crank-Nicolson linear finite element scheme applied to the linear shallow water equations in a ID domain containing an abrupt change in nodal spacing. In Part I of this series the reflection/transmission analysis was verified by some ‘hot-start’ numerical experiments. Here in Part II, however, that analysis is found wanting when it comes to providing a description of the pseudo-steady state wave configuration which develops with some ‘cold-start’ experiments. It is shown that the analysis of Part I can be extended to take in both the ‘hot-’ and ‘cold-start’ experimental results such that four essentially different wave configurations can be identified. The four configurations are discernible on the basis of group velocity. In order to be sustained, two of the configurations require one energy source whereas the other two require two energy sòurces. Numerical experiments confirmed the analysis.
    Additional Material: 14 Ill.
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  • 74
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    Chichester : Wiley-Blackwell
    International Journal for Numerical Methods in Fluids 9 (1989), S. 833-853 
    ISSN: 0271-2091
    Keywords: Fourier analysis ; Dispersion relation ; Reflected/transmitted evanescent waves ; Crank-Nicolson linear finite element scheme ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: The numerical scheme upon which this paper is based is the 1D Crank-Nicolson linear finite element scheme. In Part I of this series it was shown that for a certain range of incident wavelengths impinging on the interface of an expansion in nodal spacing, an evanescent (or spatially damped) wave results in the downstream region. Here in Part III an analysis is carried out to predict the wavelength and the spatial rate of damping for this wave. The results of the analysis are verified quantitatively with seven ‘hot-start’ numerical experiments and qualitatively with seven ‘cold-start’ experiments. Weare has shown that evanescent waves occur whenever the frequency of a disturbance at a boundary exceeds the maximum frequency given by the dispersion relation. In these circumstances the ‘extended dispersion’ relation can be used to determine the rate of spatial decay.In the context of a domain consisting of two regions with different nodal spacings, the use of the group velocity concept shows that evanescent waves have no energy flux associated with them when energy is conserved.
    Additional Material: 11 Ill.
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  • 75
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    International Journal for Numerical Methods in Fluids 9 (1989), S. 783-810 
    ISSN: 0271-2091
    Keywords: Non-uniform mesh ; Wave Reflection/transmission ; Crank-Nicolson finite elements ; Fourier analysis ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: This is the first of a series of three related papers dealing with some of the consequences of non-uniform meshes in a numerical model. In this paper the accuracy of the Crank-Nicolson linear finite element scheme, which is applied to the linear shallow water equations, is examined in the context of a single abrupt change in nodal spacing. The (in)accuracy is quantified in terms of reflection and transmission coefficients. An incident wave impinging on the interface between two regions with different nodal spacings is shown to give rise to no reflected waves and two transmitted waves. The analysis is verified using three different wavelengths (2Δx, 4Δx 8Δx) in three ‘hot-start’ numerical experiments with a mesh expansion factor of 2 and three experiments with a mesh contraction factor of 1/2. An energy flux analysis based on the concept of group velocity shows that energy is conserved across the interface.
    Additional Material: 16 Ill.
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  • 76
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    Cellular and molecular life sciences 44 (1988), S. 776-777 
    ISSN: 1420-9071
    Keywords: Fourier analysis ; growth ; selection ; size ; shape
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Fourier analysis of videodigitised outlines of mouse vertebrae from two stocks, a pseudo-longitudinal series of mice aged 25–60 days and one selected for large or small body size over many generations shows that the shape changes due to normal growth are not similar to those produced by selection for body size.
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  • 77
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    European journal of clinical pharmacology 34 (1988), S. 433-437 
    ISSN: 1432-1041
    Keywords: procaterol ; airway responsiveness ; bronchodilator ; dynamic compliance ; Fourier analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Medicine
    Notes: Summary We studied the effective site of an inhaled aerosol of procaterol, a β2-selective adrenergic bronchodilator, in 8 asthmatic patients whose basal lung functions are almost within the normal range in both slow vital capacity (VC) and forced expiratory volume in one second (FEV1.0), and are free from asthmatic attack. In patients who had received procaterol 30 min after inhalation of aerosol, there was no significant change in VC, although FEV1.0, maximal expiratory flow at 50% VC $$(\dot V_{50} )$$ , maximal expiratory flow at 25% VC $$(\dot V_{25} )$$ and maximal expiratory flow at 30% VC of partial maximal expiratory flow volume curve $$(\dot V_{30p} )$$ improved significantly. On the other hand, in those who had received placebo, none of the parameters changed. Furthermore, Rl decreased and C0.5 increased significantly during the first 5 min after inhalation of procaterol aerosol. After an interval of 5 min, Rl did not change any further, while C0.5 continued to improve until 30 min after inhalation of procaterol. These results suggest that procaterol may first dilate the large airway and then may gradually dilate the small airway in bronchial asthma.
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  • 78
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    Journal of molecular medicine 66 (1988), S. 241-245 
    ISSN: 1432-1440
    Keywords: Erythropoietin ; Stability ; Radioimmunoassay ; Polycythemic mouse bioassay ; Recombinant DNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Radioimmunoassays for erythropoietin are limited so far to a few specialized laboratories and this requires transport and storage of samples. We therefore tested the stability of immunoreactive erythropoietin in serum and plasma samples obtained from a uremic and a nonuremic anemic patient. No significant change in the concentration of immunoreactive erythropoietin was found in either serum or plasma samples for up to 14 days of storage. This type of stability was observed no matter whether the samples were stored at room temperature, 4° C, or −20° C. There was no difference between the estimates of erythropoietin in serum and heparinized plasma. Validity of the radioimmunoassay used in this study was demonstrated by parallelism of dilution curves of test specimens and the 2nd International Reference Preparation for erythropoietin and by a close correlation between the immunoreactivity and the bioactivity of the hormone, as assessed in the same samples by the exhypoxic polycythemic mouse bioassay. In conclusion the data obtained clearly indicate that the necessity of storage and transport of clinical samples does not limit the practicability of the radioimmunoassay for erythropoietin.
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  • 79
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    European archives of psychiatry and clinical neuroscience 238 (1988), S. 110-113 
    ISSN: 1433-8491
    Keywords: Schizophrenia ; Cytomegaloviruses ; Virus diseases ; Recombinant DNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Using highly sensitive nucleic acids hybridization techniques, which allow the detection of 0.1–0.5 single copy gene equivalents per cell, DNA from the temporal cortex of seven definite schizophrenics, five persons with schizophrenia-like psychoses, three patients with Huntington's chorea and nine mentally normal individuals were probed with human cytomegalovirus (HCMV) DNA. A clear hybridization signal was obtained with DNA from the temporal lobe of a young schizophrenic patient, whereas DNA from the temporal cortex of controls did not hybridize to the HCMV probe. This finding is in agreement with the cytomegalovirus hypothesis of schizophrenia and hints at the possibility that viral infection of the temporal cortex may in some sporadic cases be a contributing factor to the development of schizophrenic psychoses. There is no indication, however, that infection of the central nervous system with HCMV is an aetiological factor in the great majority of schizophrenic disorders. Clearly further studies, preferably in situ hybridizations of whole brains, are needed to prove or disprove the cytomegalovirus hypothesis of schizophrenia.
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  • 80
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    Documenta ophthalmologica 69 (1988), S. 153-159 
    ISSN: 1573-2622
    Keywords: diabetic retinopathy ; electroretinogram ; Fourier analysis ; oscillatory potentials
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Studying the oscillatory potentials in diabetic retinopathy, the authors experienced several problems interpreting results of digital filtering. The main problem was the separation of the first potential from the a-wave, since their frequencies are within the same range. To improve the procedure of measuring implicit times and of calculating amplitudes, the filtering was started with a finite impulse response filter and followed by a fast Fourier transform. The power of the oscillatory potential was calculated by determining the dominant frequency in the Fourier transformed response and expressed in microwatts. A group of normal subjects was compared with a group of early diabetic retinopathy patients. It appears that even in pathological circumstances a quantitative expression of the oscillatory potential is possible.
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  • 81
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    Documenta ophthalmologica 68 (1988), S. 283-292 
    ISSN: 1573-2622
    Keywords: Pattern-electroretinogram ; melanoma ; retinal detachment ; glaucoma ; rod-electroretinogram ; cone-electroretinogram ; Fourier analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract A comparison between results obtained by pattern electroretinography (ERG), Ganzfeld cone and rod-ERG was made in 7 patients suffering from melanoma, glaucoma and retinal detachment. It was shown that the extent of the lesions, not seen ophthalmoscopically, can be well monitored by the second harmonic component of pattern electroretinography in cases where conventional Ganzfeld ERG's do not reveal defects. Especially damage caused by acute glaucoma attacks and those stemming from chronic hypertension can be well differentiated.
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  • 82
    ISSN: 1617-4623
    Keywords: Complementary chromatic adaptation ; Gene expression ; Photoregulation ; Phycobilisome ; Recombinant DNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In cyanobacteria, light is harvested by phycobilisomes which are essentially made up of chromophoric proteins called phycobiliproteins. We have characterized two gene clusters (cpcB1, cpcA1 and cpcB3, cpcA3) each encoding the two subunits of phycocyanin (βPC and αPC, respectively), one of the major phycobiliproteins in Calothrix 7601. Downstream from the gene encoding the PCα subunit in cluster 1, an open reading frame was found, cpcE1. These genes are organized in two transcriptional units, namely: cpcB3 A3 and cpcB1 A1 E1. All these genes are transcribed whatever the chromatic light received during cell growth. Consequently, although only one type of “constitutive” PC has been biochemically characterized, we have demonstrated that there are two cpc operons “constitutively” transcribed in this strain. With the previously described red light “inducible” cpcB2 A2 operon, there are three copies of the PC encoding genes in Calothrix 7601. The significance of this newly described multigene family in cyanobacteria is discussed. We have also mapped the 5′ and 3′ termini of the major transcript from the cpc1 operon. Analysis of the 5′ untranslated region of this transcript has revealed alternative secondary structures which are proposed to play a role in the regulation of the expression of this operon.
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  • 83
    ISSN: 1617-4623
    Keywords: Autolysin ; Hydrolase ; In vitro transcription and translation ; Recombinant DNA ; Transcriptional and translational signals
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Several hundred bacterial isolates were screened for bacteriolytic activity by growing them on agar medium containing autoclaved, lyophilized Micrococcus lysodeikticus cells as the substrate. A Bacillus sp. producing the largest lytic zone was selected. A genomic bank of this selected bacterium was constructed in the multi-functional vector pTZ18R, with partial SauIIIA DNA fragments inserted at the SalI restriction site. Screening of 800 colonies of this bank for cell lysis gave 5 recombinants exhibiting lytic activity, as detected by analysis of extracts of sonicated Escherichia coli cells on denaturing polyacrylamide gels containing autoclaved, lyophilized M. lysodeikticus cells as the substrate. One clone (pBH2500), expressed inE. coli strain NM522, was found to code for a lytic enzyme corresponding, in molecular weight, to the 27 kDa Bacillus sp hydrolase. This clone with an insertion of 2.5 kb was then subcloned as a 929 bp EcoRI-SauIIIA fragment in pTZ18R (pBH929) and showed higher cell lytic activity. A unique open reading frame for a protein of 251 amino acids, followed by a putative terminator sequence, was found after a consensus ribosome binding site. A putative leader sequence was identified in the first 37 amino acids. One truncated subclone (pBH703), corresponding to 196 out of 251 residues from the protein N-terminal end, still possessed lytic activity.
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  • 84
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    Molecular genetics and genomics 215 (1988), S. 19-25 
    ISSN: 1617-4623
    Keywords: IGF-1 ; Escherichia coli secretion/export ; LamB leader peptide ; Heterologous gene expression ; Recombinant DNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The processing of LamB-IGF-1 fusion protein and the export of processed IGF-1 (insulin-like growth-factor-1) into the growth medium was examined in the Escherichia coli host strain, JM101. Several strain or plasmid modifications were tried to increase export of periplasmic (Processed) IGF-1 into the growth medium of JM101. These included: (1) use of a lon null mutant strain to increase accumulation levels of unprocessed LamB-IGF-1 fusion protein; (2) use of an alternative drug resistance marker on the expression plasmid rather than beta-lactamase, thereby reducing any competition for processing of LamB-IGF-1 by signal peptidase; (3) examination of whether phage M13 gene III protein expression caused more periplasmic IGF-1 to be exported into the growth medium due to increased outer membrane permeability; and (4) examination of the effect of E. coli or yeast optimized IGF-1 codons. None of these strain or plasmid modifications caused any significant increase in export of IGF-1 into the growth medium of JM101. Solubility studies of LamB-IGF-1 and processed IGF-1 showed that virtually all of the LamB-IGF-1 and IGF-1 remaining within the cell after a 2 h induction period was insoluble. This implied that only soluble LamB-IGF-1 was processed to IGF-1 and that only soluble IGF-1 was exported into the growth medium. Taken together, the results indicated that LamB-IGF-1 and IGF-1 solubility were the limiting factors in secretion of IGF-1 into the periplasm and export of IGF-1 into the growth medium.
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  • 85
    ISSN: 1617-4623
    Keywords: Competence ; Autolysins ; Choline ; Recombinant DNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We have worked out conditions for the study of competence development and genetic transformation in Streptococcus oralis NCTC 11427 (type strain), a species that contains choline in the cell wall. The peak of competence was found at the early exponential phase of growth and the optimal conditions for transformation were achieved with shuttle plasmids prepared from S. pneumoniae or from Escherichia coli serving as donor DNA. Transformation with dye-bouyant density gradient purified plasmid preparations followed first-order kinetics. The pneumococcal amidase can be expressed in S. oralis harbouring a plasmid carrying the lytA gene. This enzyme lysed the cell wall of the transformed cell in the presence of detergents.
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  • 86
    ISSN: 1617-4623
    Keywords: metF ; 5,10-methylenetetrahydrofolate reductase ; Recombinant DNA ; Nucleotide sequence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The Salmonella typhimurium LT2 metF gene, encoding 5,10-methylenetetrahydrofolate reductase, has been cloned. Strains with multicopy plasmids carrying the metF gene overproduce the enzyme 44-fold. The nucleotide sequence of the metF gene was determined, and an open reading frame of 888 nucleotides was identified. The polypeptide deduced from the DNA sequence contains 296 amino acids and has a molecular weight of 33 135 daltons. Mung bean nuclease mapping experiments located the transcription start point and possible transcription termination region for the gene. There is a 25bp nucleotide sequence between the translation termination site and the possible transcription termination region. This region possesses a GC-rich sequence that could form a stable stem and loop structure once transcribed (ΔG=-9 kcal/mol), followed by an AT-rich sequence, both of which are characteristic of rho-independent transcription terminators. The nucleotide and deduced amino acid sequences of the S. typhimurium metF gene are compared with the corresponding sequences of the Escherichia coli metF gene. The nucleotide sequences show 85% homology. Most of the nucleotide differences found do not alter the amino acid sequences, which show 95% homology. The results also show that a change has occurred in the metF region of the S. typhimurium chromosome as compared to the E. coli chromosome.
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  • 87
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    Molecular genetics and genomics 213 (1988), S. 269-277 
    ISSN: 1617-4623
    Keywords: Recombinant DNA ; Helix-turn-helix motifs ; In vitro transcription-translation ; Phage immunity ; Exonuclease III deletions
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The nucleotide sequence of the 3.4 kb SphI-G fragment that contained the repressor gene (c) of the temperate Streptomyces phage ϕc31 was determined. Analysis of this sequence revealed a large open reading frame with protein coding character and sequence changes in c gene point and deletion mutants identified this as the coding region of the repressor. Two of the mutants studied had undergone deletions of 1.1 kb and 1.4 kb that had occurred across short direct repeats of 6 bp and 11 bp, respectively. Coupled in vitro transcription-translation experiments using the cloned SphI-G fragment and Streptomyces lividans cell free extracts identified a protein product of approximately 72 kDa, in close agreement with that predicted from the nucleotide sequence. A strongly predicted helix-turn-helix motif that may be involved in DNA binding occurred towards the carboxy-terminus of the amino acid sequence. Initial attempts to clone the SphI-G fragment in Streptomyces failed; using information gained from the sequence analysis a smaller segment of this DNA fragment was cloned in S. lividans and conferred immunity to a clear plaque mutant (c1) of ϕc31.
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  • 88
    ISSN: 1617-4623
    Keywords: Recombinant DNA ; Chloramphenicol resistance ; cat gene ; Plasmids pC194 and pUB110 ; Inducible gene expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Two non-homologous chloramphenicol (Cm) acetyltransferase (CAT) genes, designated catA and catB, were cloned from Clostridium butyricum type strains and characterized by restriction mapping. Both genes are efficiently expressed in Escherichia coli and Bacillus subtilis. In contrast to analogous genes from staphylococci and bacilli, gene expression is not dependent on induction by Cm. The genes are considered as chromosomal, since no association with endogenous plasmids was detectable. Southern hybridization revealed a homology between catA and the staphylococcal Cm resistance plasmid, pC194. The subunit size of the clostridial CAT enzymes expressed in E. coli was determined as 22.5 kDa (catA) and 24 kDa (catB), respectively. The C. butyricum cat genes provide potentially useful selection markers for the construction of cloning vectors from cryptic clostridial plasmids.
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  • 89
    ISSN: 1432-069X
    Keywords: Collagen ; Fibroblasts ; Scleroderma ; Recombinant DNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Fibroblast cultures were started from affected and unaffacted skin areas of six patients with localized scleroderma in an active stage. The cell lines were studied for synthesis of procollagens and fibronectin by metabolic labeling with 3H-proline and for their contents of mRNAs for pro α1(I) and pro α2(I) collagen. For this purpose a cDNA clone for human pro α1(I) collagen mRNA was constructed. The clone was identified by restriction site mapping and hybridization to the specific mRNAs. All the scleroderma fibroblast lines produced increased amounts of type I and type III collagens and fibronectin during the early passages. The cell lines gradually reduced their elevated synthesis of collagen and fibronectin to normal or near normal levels by the tenth passage. The ratios of α1(I) and α2(I) chains and of type I and type III collagens, and the extent of type I procollagen processing, remained relatively unchanged in all the cultures. The cellular levels of type I procollagen mRNAs were increased in all the cells exhibiting an increased synthesis of collagen. The results suggest that in localized scleroderma the fibroblasts have undergone a coordinated activation of collagen synthesis at transcriptional level.
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  • 90
    ISSN: 1432-0983
    Keywords: Schizophyllum commune ; Transformation ; Gene isolation ; Basidiomycetes ; Recombinant DNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We have developed a routine way to isolate genes directly from the basidiomycete fungus, Schizophyllum commune. Plasmid DNA from a genomic gene library was used to isolate five specific genes by complementation of Schizophyllum mutations via transformation. The mutant strains were deficient in the ability to synthesize either adenine (ade2 and ade5), uracil (ural, encoding orotidine-5′-phosphate decarboxylase; OMPdecase), tryptophan (rpl, encoding indole-3-glycerol phosphate synthetase; IGPS) or para aminobenzoic acid (pab1). In each case, Southern analysis revealed that transformation to prototrophy was concomitant with the integration of vector sequence into the genome of the S. commune mutant. Total DNA from transformants was restricted, religated, and used to transform E. coli. Ampicillin resistant plasmids were recovered from E. coli and tested for their ability to transform the corresponding mutant of S. commune. Plasmids complementing the ade2, adeS, pabl, trpl, and ural mutations were recovered.
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  • 91
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    Journal of industrial microbiology and biotechnology 2 (1987), S. 117-121 
    ISSN: 1476-5535
    Keywords: Fermentation ; Recombinant DNA ; Hepatitis B surface antigen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Fermentations were performed to determine parameters affecting the expression of hepatitis B surface antigen (HBsAg) in the yeastSaccharomyces cerevisiae containing the HBsAg gene. These studies emphasized inereasing both the relative abundance (HBsAg: cell mass) and total production of HBsAg. Specific activity was increased 70-fold when cells were grown in shake flasks containing nonselective rather than selective medium. The addition of adenine, ammonium sulfate or glucose to the complex medium reduced the production of antigen. Results similar to those achieved in shake flasks were obtained when the growth was performed in fermenters. A nutrient addition system was employed to increase the production of cells and HBsAg. The addition of glucose to the culture medium increased cell mass 6-fold but decreased the production of antigen. This imbalance was corrected by supplementing the glucose with complex nutrients.
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  • 92
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    Journal of industrial microbiology and biotechnology 1 (1987), S. 277-282 
    ISSN: 1476-5535
    Keywords: Recombinant DNA ; Gene expression ; Genetic engineering ; Biotechnology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary A variety of factors affect the expression of foreign proteins inEscherichia coli. These include: promoter strength, efficiency of ribosome binding, stability of the foreign protein inE. coli, location of the foreign protein inE. coli, the codons used to encode the foreign protein, the metabolic state of the cell, and the location, stability and copy number of the foreign gene. This paper contains a critical review of these factors with the idea that a detailed understanding of them is the key to the development of strategies for the efficient large-scale production of foreign proteins inE. coli.
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  • 93
    Electronic Resource
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    Plant and soil 99 (1987), S. 185-196 
    ISSN: 1573-5036
    Keywords: Antibody ; H+-ATPase ; Membrane ; Recombinant DNA ; Transport
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Mineral transport across the plasma membrane of plant cells is controlled by an electrochemical gradient of protons. This gradient is generated by an ATP-consuming enzyme in the membrane known as a proton pump, or H+-ATPase. The protein has a catalytic subunit of Mr=100,000 and is a prominent band when plasma membrane proteins are analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. We generated specific rabbit polyclonal antibody against the Mr=100,000 H+-ATPase and used the antibody to screen λgtll expression vector libraries of plant DNA. Several phage clones producing immunoreactive protein, and presumably containing DNA sequences for the ATPase structural gene, were isolated and purified from a carrot cDNA library and a Arabidopsis genomic DNA library. These studies represent our first efforts at cloning the structural gene for a plant plasma membrane transport protein. Applicability of the technique to other transport protein genes and the potential for use of recombinant DNA technology in plant mineral transport research are discussed.
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  • 94
    ISSN: 1617-4623
    Keywords: Recombinant DNA ; Methionine metabolism ; Negative control ; Regulatory regions
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In Saccharomyces cerevisiae, the expression of several genes implicated in methionine biosynthesis is coregulated by a specific negative control. To elucidate the molecular basis of this regulation, we have cloned two of these genes, MET3 and MET25. The sequence of MET25 has already been determined (Kerjan et al. 1986). Here, we report the nucleotide sequence of the MET3 gene along with its 5′ and 3′ flanking regions. Plasmids bearing different deletions upstream of the transcribed region of MET3 were constructed. They were introduced into yeast cells and tested for their ability to complement met3 mutations and to respond to regulation by exogenous methionine. The regulatory region was located within a 100 bp region. The sequence of this regulatory region was compared with that of MET25. A short common sequence which occurs 250–280 bp upstream of the translation initiation codon of the gene was found. This sequence is a good candidate for the cis-acting regulatory element.
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  • 95
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    Molecular genetics and genomics 209 (1987), S. 570-574 
    ISSN: 1617-4623
    Keywords: Recombinant DNA ; Methylase ; Plasmid vector ; Gel electrophoresis ; Clearage map
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The modification genes of Flavobacterium okeanokoites and Haemophilus galinarum have been cloned into the vector pBR322 and expressed in Escherichia coli cells. FokI methylase gene is contained on a 3.80 kb piece of F. okeanokoites DNA. Plasmid constructs carrying this fragment of DNA are resistant to digestion by FokI restriction endonuclease but are sensitive to cleavage by HindIII, EcoRI and PstI. Unmodified λ DNA molecules, exposed in vitro to cell extracts prepared from cells habouring this plasmid, became resistant to digestion by FokI. The smallest HgaI methylase clone carries the pBR322 plasmid containing a 3.50 kb piece of H. galinarum DNA. This plasmid is resistant to digestion by HgaI. Neither the FokI nor the HgaI restriction endonuclease was detected in either clone. This is the first report of cloning modification genes whose protein products recognise asymmetric nucleotide sequences.
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  • 96
    ISSN: 1619-7089
    Keywords: First pass ventriculography ; Coronary artery disease ; 99mTc-DTPA ; Cardiac functional images ; Fourier analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Rest and exercise radionuclide ventriculograms were performed in 61 non infarcted, male, patients who underwent cardiac catheterization for chest pain and in 16 normal control subjects. Studies were performed using the first pass method with a fast single crystal gamma camera, which allowed a count rate of 140±19 Kcounts/sec to be reached during left ventricular filling; the count integral on left ventricular area was 10.8±1.6 Kcounts and the maximum count/pixel 155±16. We analyzed sensitivity, specificity, positive and negative predictive value of global ejection fraction (EF) and of the regional wall motion in identifying ventricular function abnormalities due to obstructive coronary artery disease. The regional wall motion was evaluated with four functional images: regional ejection fraction (REF), amplitude (A) and phase (PH) from Fourier analysis and systolic transit times (TT). Sensitivity was near 90% for EF, REF, A and TT, while PH was less sensitive (80%); all functional images were more specific (nearly 90%) than EF (80%). Both sensitivity and specificity were lower for the exercise EKG (59% and 63%, respectively) in this patient group. Significant differences between single vessel and multiple vessel disease were also observed either for the EF increase/decrease (-1.34±7.4 and-7.82±9.96; P〈0.05) or for the number of segments which developed wall motion abnormalities during exericise (1.22±0.73 and 2.15±1.04; P〈0.02). In conclusion, with our method, a fast single crystal gamma camera is suitable for obtaining optimal first pass radionuclide ventriculograms with a count density sufficient either for global or regional left ventricular function evaluation. First pass radionuclide ventriculography seems to provide very high diagnostic accuracy in the detection of cornorary artery disease in non infarcted, male, subjects.
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  • 97
    ISSN: 1619-7089
    Keywords: Fourier analysis ; Orthogonal factor analysis ; Factor analysis of dynamic structures ; Cardiac phantom
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Orthogonal and oblique factor analysis represent an alternative to Fourier analysis in the evaluation of cardiac dynamic behaviour in gated blood pool studies. In order to estimate their respective places, orthogonal factor analysis (OFA), factor analysis of dynamic structures (FADS) and Fourier analysis (FA) are tested on a dynamic and periodical phantom with well known and reproducible kinetics. The phantom data are acquired under standard conditions by varying the counting rates and the temporal frequency sampling. To compare the results of the three methods with maximal objectivity, the relative contribution of each component is calculated. With standard acquisition conditions, FA and OFA give very close results. Only a minor advantage in evaluation of small phase differences is observed with OFA. FADS solutions are effectively related to the dynamic behaviour of the phantom, but their interpretation is more complicated and the quality of the oblique factors is reduced as the number of calculated factors increases. The influence of the counting statistics on FA, OFA and FADS is very similar. However, in cases of undersampling, robustness is demonstrated with the factorial technics.
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  • 98
    ISSN: 1432-0983
    Keywords: Recombinant DNA ; Cross hybridization ; 2D-electrophoresis ; Hybrid selection translation ; Transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Using the structural gene for the ribosomal protein L3 from Saccharomyces cerevisiae as a probe, we isolated a homologous fragment from genomic DNA of Schizosaccharomyces pombe. Analysis of the plasmid carrying this fragment by hybridization selection and 2D-electrophoresis revealed a 31 kDa ribosomal protein. Transformation of the vector pDB248x containing this fragment into Schizosaccharomyces pombe leads to an increased level of mRNA suggesting that we have cloned the entire and actively transcribed gene.
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  • 99
    ISSN: 1432-0983
    Keywords: Mitochondrial DNA ; Population heterogeneity ; Molecular evolution ; Recombinant DNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Physical characterization of the mitochondrial genome derived from the obligate mosquito parasite, Romanomermis culicivorax has generated some surprising physical properties regarding the molecular structure of nematode mitochondrial DNA (mtDNA). Restriction enzyme analysis of this mtDNA has revealed a mitochondrial genome size of approximately 26 kb, the largest metazoan mtDNA reported to date. Isofemale lineages are monomorphic for one of three size variants, differing by 500-1,000 base pairs, present in our original field population. Cloned hybridization probes derived from a single region exhibiting a 600 by size polymorphism share strong homology with several spatially separated sites distributed about the mtDNA. This suggests that the homology is a result of repeated DNA sequence elements contained within this mitochondrial genome that contribute to mtDNA size polymorphism.
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  • 100
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    Experimental brain research 64 (1986), S. 5-18 
    ISSN: 1432-1106
    Keywords: Striate cortex ; Fourier analysis ; Receptive fields
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Simple cells in the macaque striate cortex were tested with bars, edges and gratings. Spatial frequency tuning curves could be predicted from the spatial profiles plotted with bars and edges and the bandwidth could be evaluated more accurately by computing the mean from measured and predicted tuning curves. The results suggest that the mean relative spatial frequency bandwidth (Δf/fo) is nearly constant and of a moderate value. But at each optimal spatial frequency, cells with different bandwidths (about a factor of two) were recorded. The shapes of spatial response profiles resemble the corresponding spatial and spatial frequency characteristics of line and edge detectors evaluated psychophysically. Among the remaining cell types, concentric cells tend to be tuned to lower spatial frequencies and have broader bandwidths, whereas periodic cells prefer higher spatial frequencies and have narrower bandwidths. Thus the mean relative bandwidth tends to decrease significantly with spatial frequency (as required by a system of patch-by-patch Fourier analysis) only when cells with poor orientation selectivity and the non-linear silent periodic cells are included along with the simple cells. Simple cells, on their own, seem to form a quasi-linear contrast processing system which is more biased towards spatial accuracy than spatial frequency selectivity.
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