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  • Articles: DFG German National Licenses  (205)
  • 1985-1989
  • 1970-1974  (205)
  • 1965-1969
  • 1945-1949
  • 1973  (205)
  • Electron microscopy  (149)
  • Rat  (66)
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  • Articles: DFG German National Licenses  (205)
Material
Years
  • 1985-1989
  • 1970-1974  (205)
  • 1965-1969
  • 1945-1949
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Keywords
  • 1
    Electronic Resource
    Electronic Resource
    The contribution of solvent drag to the intestinal absorption of tritiated water and urea from the jejunum of the rat (1973)
    Springer
    Naunyn-Schmiedeberg's archives of pharmacology 279 (1973), S. 133-152 
    Details
    ISSN: 1432-1912
    Keywords: Intestinal Absorption ; Tritiated Water ; Urea ; Solvent Drag ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary 1. Jejunal loops of anaesthetized rats were perfused with hypotonic, isotonic, and hypertonic buffered solutions containing tritiated water or 14C-labelled urea. The blood flow of the loop was maintained constant at an intermediate rate (0.8–0.9 ml min−1 g−1 wet tissue weight). The water net flux was determined by means of polyethylene glycol as nonabsorbable marker and amounted up to ±30 μl min−1 g−1 w. w. 2. A positive water net flux (directed towards the blood) increased the absorption rates (disappearance rate from the intestinal lumen, appearance rate in the intestinal venous blood) of tritiated water and urea by maximally 22 and 41%, a negative water net flux (directed towards the gut lumen) diminished it by 12 and 32%. 3. The data were analysed by means of a kinetic model with the water net flux as independent variable. The sieving coefficient ⧫=1-δ was 1.18±0.44 for tritiated water and 0.86±0.11 for urea at pH 6.2. The water net flux influenced the absorption of solutes by interaction within the membranes (solvent drag in a restricted sense) and by changing the volume of blood flowing in the capillaries near the epithelium. This last effect is large for the absorption of the rapidly absorbable tritiated water, but small for the absorption of urea which is absorbed ten-times slower. 4. The water net flux in the intestine depended linearly on the osmotic pressure difference between lumen and blood. The hydraulic permeability was 146–180 ml2 min−1 g−1 osm−1.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00503979
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  • 2
    Electronic Resource
    Electronic Resource
    The influence of blood flow on the absorption of l- and d-phenylalanine from the jejunum of the rat (1973)
    Springer
    Naunyn-Schmiedeberg's archives of pharmacology 277 (1973), S. 113-138 
    Details
    ISSN: 1432-1912
    Keywords: Intestinal Blood Flow ; Intestinal Absorption ; l- and d-phenylalanine ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary 1. Jejunal loops of anaesthetized rats were perfused (single pass and recirculation) with buffered solutions containing l-phenylalanine (about 30 μM and 30 mM) and d-phenylalanine (about 30 μM). The blood flow, the disappearance rate (from the intestinal lumen) and the appearance rate (in the intestinal venous blood) were determined simultaneously, the accumulation in the gut wall at the end of the experiments. 2. At a constant intermediate blood flow the absorption of l- and d-phenylalanine decreased with time. A decrease of blood flow from about 1.6 to 0.25 ml min−1g−1 wet tissue weight diminished the appearance and the disappearance rate of both isomers. An increase of blood flow from 0.25 to 1.6 ml min−1g−1 caused no increase of absorption; on the contrary the appearance and disappearance rate decreased a little. The different dependence of absorption when decreasing and increasing the blood flow rate may be caused by a changed pattern of blood flow in the layers of the gut wall, i.e. the flow rate in the villi may be reduced while the flow rate in the entire wall remains unchanged. 3. In the single pass perfusion experiments the appearance rate of l-phenylalanine increased proportionally to the luminal concentration (30 μM and 30 mM), while the accumulation was relatively smaller at the high concentration. In the recirculation experiments the appearance rate and the accumulation of l-phenylalanine did not increase proportionally. 4. The increase in perfusion rate, from 0.1 (single pass perfusion) to 2–3 and 10 ml min−1 (recirculation), caused an increase of l-phenylalanine absorption and tissue accumulation. 5. At a luminal concentration of about 30 μM the appearance rates of l- and d-phenylalanine were equal, while the tissue accumulation was smaller for the d-isomer.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00501154
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  • 3
    Electronic Resource
    Electronic Resource
    Licht- und elektronenmikroskopische Untersuchungen an der Zona glomerulosa des Hundes nach Behandlung mit Spirolacton (1973)
    Springer
    Research in experimental medicine 159 (1973), S. 321-332 
    Details
    ISSN: 1433-8580
    Keywords: Spirolactone ; Aldosterone ; Zona glomerulosa ; Electron microscopy ; Spirolacton ; Aldosteron ; Zona glomerulosa ; Ultrastruktur
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Die Wirkung von Spirolacton auf die Zona glomerulosa des Hundes wurde licht- und elektronenmikroskopisch untersucht. Die an Kontroll- und Versuchstieren erhobenen Befunde werden miteinander verglichen. 1. Lichtmikroskopisch werden bei Kontroll- und Versuchstieren zwei unterschiedlich stark anfärbbare Zelltypen beobachtet. Bereits nach 10tägiger Behandlung mit Spirolacton läßt sich eine beträchtliche Dickenzunahme der Zona glomerulosa nachweisen. Mit den angewandten Färbemethoden lassen sich in den beiden Zelltypen keine den „Spironolacton-Körpern“ ähnliche Einschlüsse darstellen. 2. Elektronenmikroskopisch zeigen die beiden Zelltypen deutliche Unterschiede in der Feinstruktur; wegen ihrer unterschiedlichen elektronenoptischen Dichte werden sie als helle und dunkle Zellen bezeichnet. Im Cytoplasma der dunklen Zellen kommen 1–4 lamelläre Körper vor, die jedoch nicht den beim Menschen beobachteten „Spironolacton-Körpern“ ähnlich sind. Nach Behandlung mit Spirolacton weisen die hellen und die dunklen Zellen (einschließlich ihrer lamellären Körper) gegenüber Kontrolltieren keine feinstrukturellen Unterschiede auf. Es wird diskutiert, ob die hellen und dunklen Zellen in der Zona glomerulosa unterschiedliche Aktivitätszustände ein und derselben Zellart darstellen. Ebenso wird die mögliche Funktion der lamellären Körper im Vergleich zu den Ergebnissen anderer Autoren erörtert.
    Notes: Summary The effect of spirolactone on the zona glomerulosa was studied in dogs by light and electron microscopy. Results of untreated animals are compared with those pretreated with spirolactone for several days. Light microscopy in control as well as in experimental animals disclosed two different types of cells as far as their staining properties were concerned. With the staining techniques utilized in this study cellular inclusions, so-called spironolactone bodies as previously reported by others, were not seen. The width of the zona glomerulosa, however, was found to be significantly increased in treated animals. Electron microscopy revealed the two respective cell types to be definitely different. Due to their electron density they are referred to her as light and dark cells. Within the cytoplasm of the dark cells laminated inclusions were seen, ranging in size from 1 to 4 µm. These inclusions are thought to be not identical with socalled spironolactone bodies. Spirolactone did not affect the ultrastructural appearance of either light or dark cells, when compared with control, this is also true for the laminated inclusions. It is suggested that the light and dark cells represent the same type of cell at different activity levels. Finally, the functional significance of the laminated inclusions is discussed in view of findings reported elsewhere.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01851606
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  • 4
    Electronic Resource
    Electronic Resource
    Untersuchungen zur Bildung gemischter Disulfide zwischen D-Penicillamin und Serumproteinen (1973)
    Springer
    Research in experimental medicine 161 (1973), S. 289-297 
    Details
    ISSN: 1433-8580
    Keywords: D-penicillamine ; L-cysteine ; Serum proteins ; Rat ; D-Penicillamin ; L-Cystein ; Serumproteine ; Ratte
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung In vitro-Untersuchungen zeigten, daß D-Penicillamin durch Serumproteine, in erster Linie durch Albumin, gebunden wird. Aller Wahrscheinlichkeit nach ist die Bildung gemischter Disulfide hierfür verantwortlich. Cystin, L-Cystein und andere Thiole sowie N-Äthylmaleinimid hemmen die Proteinbindung von D-Penicillamin. Verabfolgt man Ratten vor Injektion des D-Penicillamins L-Cystein, resultiert eine vorübergehende Erhöhung des Gehalts an freiem D-Penicillamin in Serum und in einigen Geweben, während die Ausscheidung mit dem Urin verzögert wird. Die theoretische und praktische Bedeutung der Befunde wird diskutiert.
    Notes: Summary Studiesin vitro show that D-penicillamine is bound by serum proteins, mainly albumin; the formation of a mixed disulfide is the most likely explanation. Cystine, L-cysteine and other thiols as well as N-ethylmaleinimide were found to inhibit the protein binding of D-penicillamine. Administration of L-cysteine to rats prior to D-penicillamine brings about a transient increase of the amount of free D-penicillamine in serum and in some tissues as well as a delay in its urinary excretion. The theoretical and practical implications of the findings are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01851452
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  • 5
    Electronic Resource
    Electronic Resource
    Morphologie und Funktion isoperistaltischer, ausgeschalteter Jejunalschlingen nach Rückverlegung in die Dünndarmpassage (1973)
    Springer
    Research in experimental medicine 161 (1973), S. 133-140 
    Details
    ISSN: 1433-8580
    Keywords: Self-emptying loop ; Mucosal atrophy ; Small intestine ; Luminal nutrition ; Morphology ; Glucose-absorption ; Rat ; Selbstentleerende Blindschlingen des Jejunums ; Schleimhautatrophie ; Topische Ernährung ; Morphologie ; Glucoseresorption ; Ratte
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Um Möglichkeit und Ausmaß der Reversibilität einer Dünndarmschleimhautatrophie zu prüfen, wurden operativ ausgeschaltete, atrophische Jejunalschlingen nach Rückverlegung in die Dünndarmpassage morphologisch und funktionell untersucht. Ergebnisse: 1 Monat nach Rückverlegung hatte sich die Schleimhautatrophie zurückgebildet. Morphometrisch fand sich darüber hinaus eine Hypertrophie aller Wandschichten. Der Schleimhauthypertrophie entsprach eine Steigerung der Glucoseresorption.
    Notes: Summary Self-emptying blind loops of the small intestine in the rat were restored to functioning intestine in order to investigate the reversibility of mucosal atrophy. Results: 1 month after restoration of normal anatomical conditions atrophy was no longer demonstrable. In addition, the morphometric data indicated mucosal hypertrophy and increase in thickness of all layers of the intestinal wall. These changes were associated with a significant increase of glucose-absorption.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01855105
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  • 6
    Electronic Resource
    Electronic Resource
    Distribution of hydrolytic enzymes in early rat and mouse embryos — A reappraisal (1973)
    Springer
    Anatomy and embryology 139 (1973), S. 119-126 
    Details
    ISSN: 1432-0568
    Keywords: Cleavage stages ; Egg-cylinder ; Mouse ; Rat ; Hydrolytic enzymes distribution ; Differentiation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The time of appearance and the distribution of alkaline and acid phosphatase and nonspecific esterase was investigated in cleavage and early postimplantation stages of mouse and rat embryos. Alkaline and acid phosphatase appeared for the first time in 8-cell embryos. Activity of both enzymes grew progressively stronger to blastocyst stage. Acid phosphatase activity was revealed in the form of fine and coarse granules distributed evenly in the cytoplasm. Alkaline phosphatase was predominantly localized in plasma membranes. There was no difference in intensity of reaction between trophoblastic cells and the inner cell mass. After implantation acid phosphatase was localized in coarse granules in the apical portion of entodermal cells. With the appearance of mesoderm, the cells of embryonal entoderm became flattened and devoid of acid phosphatase activity which was restricted to cells of extraembryonic entoderm. The activity of nonspecific esterase was not detected in preimplantation stages. In postimplantation embryos it roughly corresponded to the activity of acid phosphatase. Alkaline phosphatase was localized in cell membranes of ectodermal cells. The mesodermal cells of mouse embryo displayed a somewhat weaker activity than ectodermal cells, while in the rat embryo the same layer remained completely nonreactive. Our findings on the distribution of the enzymes mentioned did not reveal any kind of polarity or bilateral symmetry in preimplantation stages. In postimplantation stages acid phosphatase and nonspecific esterase are probably bound to lysosomes and play an important role in embryonic nutrition. The absence of alkaline phosphatase from entodermal cells is somewhat puzzling and suggests that the process of molecular transport in those cells is most probably restricted to endocytosis. Our results suggest that all blastomeres are identical with respect to enzyme distribution and that the first signs of differentiation of enzyme content appear with the formation of germ layers.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00523633
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  • 7
    Electronic Resource
    Electronic Resource
    Die Entwicklung des Bewegungsapparates der Rattenzunge im Licht- und Elektronenmikroskop (1973)
    Springer
    Anatomy and embryology 140 (1973), S. 153-171 
    Details
    ISSN: 1432-0568
    Keywords: Rat ; Tongue ; Myogenesis ; Neurogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Um die Entwicklung des Bewegungsapparates der Rattenzunge im Verhältnis zu morphogenetischen Vorgängen im Gesichtsbereich, besonders zur Gaumenentwicklung, beurteilen zu können, wurde die Zungenanlage des Rattenembryos ab Tag 13 p.c. bis Tag 6 p.p. lichtmikroskopisch, histochemisch und elektronenmikroskopisch untersucht. In einem Blastem von embryonalen Mesenchymzellen, Capillaren und Neuritenbündeln differenzieren sich ab Tag 15 p.c. Myoblasten, die miteinander zu mehrkernigen Muskelfasern verschmelzen. In deren Cytoplasma bilden sich an Ribosomenketten Myofilamente, die zu Myofibrillen mit Z-Streifen organisiert werden. Ab Tag 19 p.c. ordnet sich die Querstreifung zu Registern, das Transversolongitudinalsystem lagert sich an. Neuritenbündel legen sich quer zur Längsachse an die Muskelfasern an und ab Tag 17 p.c. finden sich myoneuronale Kontakte. Typische motorische Endplatten wurden bis Tag 6 p.p. nicht gefunden. Zum Zeitpunkt des Gaumenschlusses am Tag 17 p.c. sind geordnete Muskelfasersysteme, primitive myoneuronale Kontakte mit Acetylcholinvesikeln und ein typisches Enzymmuster der Acetylcholinesterase ausgebildet. Damit bestehen vor Schluß des harten Gaumens die strukturellen und enzymatischen Voraussetzungen für eine gerichtete Bewegung der Zunge. Die Hypothese, daß motorische Aktivität zum Gaumenschluß notwendig sei, wird mit Beschreibungen embryonaler Bewegungen, mit der Literatur klinisch-pathologischer Befunde der Gaumenspalte und teratologischen Experimenten verglichen. Danach scheint ein direkter kausaler Zusammenhang zwischen Bewegungen im Kopfbereich und Gaumenschluß nicht vorzuliegen.
    Notes: Summary In order to be able to evaluate the development of the motor system of the rat tongue with respect to the morphogenetic processes in the region of the face, especially palate development, we studied the tongue anlage of the rat embryo from day 13 p.c. to day 6 p.p. histochemically and under the light and electron microscope. From day 15 p.c. onwards myoblasts differentiated out of a blastema consisting of embryonic mesenchymal cells, capillaries and neurite bundles. The myoblasts then fused together to form multinuclear muscle fibres. On the ribosome chains in the cytoplasm myofilaments were formed, which were then organized to myofibrils with Z-lines. From day 19 p.c. onwards the cross-striations came to lie next to each other and the transversolongitudinal system was added. Neurite bundles were added at right angles to the longitudinal axis of the muscle fibres, and from day 17 p.c. myoneural contacts were found. No typical motor endplates were found up to day 6 p.p. At the time of palate closure on day 17 p.c. regular muscle-fibre systems, primitive myoneural contacts with acetylcholine vesicles and a typical acetylcholine-esterase enzyme pattern had been developed. Therefore before closure of the hard palate the structural and enzymatic preconditions existed for a coordinated movement of the tongue. The hypothesis that motor activity is necessary for palate closure is compared with descriptions of embryonic movements, with clinical-pathological results in papers on the cleft palate and with teratogenic experiments. In this respect there does not seem to be any direct causal connection between movements in the head region and palate closure.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00520328
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  • 8
    Electronic Resource
    Electronic Resource
    The cortical plate and molecular layer of the late rat fetus (1973)
    Springer
    Anatomy and embryology 141 (1973), S. 3-37 
    Details
    ISSN: 1432-0568
    Keywords: CNS ; Cortical plate ; Neuroblasts ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In the cortical plate of the late prenatal rat fetus the neuroblasts can be considered to be of three types: mature neuroblasts which are prominent in the lower levels of the cortical plate and have some of the cytoplasmic and nuclear features of neurons, immature neuroblasts that have recently completed their migrations into the cortical plate, and migrating neuroblasts that are still in the process of moving to their definitive positions. Both of these latter types have darker cytoplasm than the mature neuroblasts. All of the neuroblasts have an apical process that extends directly towards the pial surface of the cortical plate and a basal process that is directed towards the intermediate zone of the developing hemisphere. In Golgi preparations some of these basal processes, particularly those of neuroblasts situated in the lower levels of the cortical plate, seem to have formed axons that pass through the intermediate zone to enter the developing white matter, in which they turn at right angles away from, and rarely toward, the midline. Other elements traversing the cortical plate are the ascending processes of spongioblasts that branch in the molecular layer and form expansions at the surface of the hemisphere. In the molecular layer the spongioblast terminal branches intertwine with the apical tufts of the ascending neuroblast processes and with thin processes that have the features of axons, to form a loose neuropil. In the cortical plate the spongioblast processes are usually closely and preferentially surrounded by the dark migrating neuroblasts and by the immature neuroblasts. Both of these latter may partially encompass spongioblast processes. Hence it is concluded that the spongioblast processes act as guides along which the migrating neuroblasts ascend through the cortical plate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00523363
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  • 9
    Electronic Resource
    Electronic Resource
    The fusion of the endocardial cushions in the heart of the chick embryo (1973)
    Springer
    Anatomy and embryology 141 (1973), S. 55-75 
    Details
    ISSN: 1432-0568
    Keywords: Heart ; Endothelial cushion ; Fusion ; Cell interaction ; Electron microscopy ; Chick embryo
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In an investigation concerning the so-called “fusion” of the atrioventricular endocardial cushions in the heart of the chick embryo the following facts were established: 1. The endocardial cushions in the chick embryo do really fuse. However, the region of fusion is less extensive than is generally accepted. 2. No regressive cellular changes were observed in the fusing endothelia with light or electron microscopy. On the contrary, mitotic activity and possibly even erythroblastic transformation were observed in the endothelium as well as in the subendothelial mesenchyme. 3. Under the light microscope, the process of fusion is characterized by interdigitation of the endothelial cells. In later stages the endothelial character of these cells disappears in such a way as to give rise to one single mesenchymal cushion mass. 4. Under the electron microscope the fusing endothelial cells form extremely long tonguelike cytoplasmic processes which protrude freely into the lumen where they may fuse with similar processes from other endothelial cells of the same side. In this way small compartments become segregated from the lumen. the plasma membrane of the processes may also fuse with the plasma membrane of the cell from which they originate. Alternating persistence and disappearance of the plasma membrane gives rise to many membrane-lined intracellular vesicles. 5. The numerous myelin figures found in and between the endothelial surfaces appear to represent the blind and empty membranous linings stripped off from cytoplasmic processes. The intracellular myelin figures are probably incorporated into the cells in the same way as described above. Apart from this form of endocytosis, signs of conventional micropinocytotic activity are present. 6. The density of endoplasmic reticulum suggests intensified protein synthetic activity, in which membrane-bound as well as free ribosomes and polysomes seem to participate. Moreover, increased mitochondrial density in the cells in the fusion region is evident. 7. Specialized junctional complexes between the plasma membranes in the form of desmosome-like junctions are relatively scarce. 8. Fusion of cytoplasmic tongues of endothelial cells with adjacent endothelial cells of the same side or of the opposite side is responsible for the exchange of large quantities of cytoplasma between endothelial cells and, as a consequence, for displacement of cell boundaries without loss of cell individuality.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00523365
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  • 10
    Electronic Resource
    Electronic Resource
    Ultrastructural observations on cell necrosis during formation of the neural tube in mouse embryos (1973)
    Springer
    Anatomy and embryology 141 (1973), S. 251-264 
    Details
    ISSN: 1432-0568
    Keywords: Closure of neural tube ; Cell necrosis ; Normal mouse embryos ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Spontaneous cell death in the developing brain of 8.5–9 day old mouse embryos has been investigated with the electron microscope. Before closure of the neural tube, areas of cell death are found at the neuro-somatic junction. After closure of the neural tube degenerating cells are found in the dorsal midline of the prospective diencephalon. Ultrastructurally, cell degeneration is marked by chromatin condensation, increase in electron density of the structures in the cytoplasm and, in later stages, by a marked pycnosis of the dying cell. After fragmentation, the necrotic material as well as entire pycnotic cells are phagocytized and digested by cells of the neuroepithelium. An invasion of macrophages has not been observed. The significance of cell necrosis is discussed in relation to the normal formation of the neural tube and to the occurrence of certain induced malformations (exencephalies).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00519046
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  • 11
    Electronic Resource
    Electronic Resource
    Über ein transitorisches, perinatales subependymales Zellsystem der weißen Ratte (1973)
    Springer
    Anatomy and embryology 141 (1973), S. 275-288 
    Details
    ISSN: 1432-0568
    Keywords: Cerebral ventricles ; Perinatal period ; Subependymal cells ; Rat ; Light and electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Die Gehirne von 93 Ratten (letzte Embryonalwoche bis zum 30. postnatalen Tag) wurden licht- und elektronenmikroskopisch untersucht. Am Ende der zweiten Embryonalwoche treten im mittleren Teil des III. Ventrikels Zellen auf, deren Cytoplasma sich mit einer Perjodsäure-Bisulfit-Aldehydthionin-Methode blau anfärben läßt. Die blauen Zellen werden nach der Geburt auch an anderen Abschnitten des III. Ventrikels sowie am Aquaedukt und IV. Ventrikel ausschließlich in einer lockeren subependymalen Gliazone gefunden. Zwischen dem 14. und 17. Tag nimmt ihre Zahl ab, um den 22. postnatalen Tag sind die blauen Zellen völlig verschwunden. Gleichzeitig wird die subependymale Auflockerungszone dichter. Elektronenmikroskopisch werden die blauen Zellen als fortsatzreiche Elemente identifiziert, die viele Mitochondrien und unterschiedlich große Vacuolen und Cytosomen enthalten. Die Fortsätze umschließen subependymale Räume, die ein feinflockiges Material enthalten. Die Zellen werden als mikrogliäre Elemente angesehen, ihre Funktion mit dem Verschwinden der subependymalen Auflockerungszone in Verbindung gebracht.
    Notes: Summary The brains of 93 rats (of between the last week of pregnancy up to the 30th postnatal day) were investigated by means of light and electron microscopy. At the end of the second week of pregnancy, subependymal cells, the cytoplasm of which is blue after staining with a periodic-acid-bisulfit-aldehydethionine-method, begin to appear in the middle part of the IIIrd ventricle. After birth, the blue cells can also be demonstrated in other parts of the IIIrd ventricle as well as in the wall of aquaeduct and the IVth ventricle. The cells are exclusively found in a loosely textured subependymal tissue. Between the 14th and the 17th day the number of the blue cells decreases and around the 22nd postnatal day they disappear completely. Simultaneously, the texture of the subependymal tissue becomes more compact. The blue cells can be identified by electron microscopy. They are characterized by the presence of several processes and contain many mitochondria, different sized vacuoles and cytosomes. The cell processes enclose subependymal cavities containing a loose flocculose material. The cells are considered as microglia cells. Their function is considered in relation to the disappearance of the loose texture of the subependymal zone.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00519048
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  • 12
    Electronic Resource
    Electronic Resource
    The cytology of neurons and their dendrites in the simple mammalian nucleus lateralis an electron microscope study (1973)
    Springer
    Anatomy and embryology 141 (1973), S. 289-317 
    Details
    ISSN: 1432-0568
    Keywords: Cerebellum ; Synapses ; Rat ; Interneuron ; Axons ; Perikarya ; Neuroglia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Profiles of large neurons in the lateral nucleus range from 16 μm to 35 μm in diameter with dimpled nuclei, large Nissl bodies, and well developed Golgi apparatus. Two types of perikarya are distinguished, those that are smooth and those with irregular somatic and dendritic protuberances. About 86% of all large neuronal somata are covered with axosomatic synapses, predominantly with terminals of Purkinje axons and a few belonging to axons of the small neurons. The remaining 14% have no axosomatic synapses. The thick, fleshy dendrites of these cells are covered with terminals, the majority of which synapse directly upon the dendritic shaft. A few are present on spines. The initial segment of the large neuron is thick and robust and receives synapses upon its shaft or upon a spinous projection. The small neurons measure less than 12 μm in diameter and have very lobulated nuclei in a sparse cytoplasm characterized by small Nissl bodies and a poorly elaborated Golgi apparatus. About 52% of all small neuronal somata bear no synapses whereas the remaining 48% are covered with axosomatic synapses, mainly from the axons of Purkinje cells and a few axons of other small cells. The slender long dendrites of both large and small cells bear synapses with six classes of axons in the neuropil. Synaptic protuberances of two varieties occur on the surfaces of both perikarya and dendrites, (a) dome-shaped ones capped with a pronounced asymmetrical synaptic junction and (b) ones with thin long necks and bulbous heads having synapses on both parts. Frond-like dendritic excrescences are borne on the processes of some small and large neurons and they are postsynaptic to many axon terminals clustered around them.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00519049
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  • 13
    Electronic Resource
    Electronic Resource
    Studies on dentinogenesis in the rat (1973)
    Springer
    Anatomy and embryology 142 (1973), S. 103-115 
    Details
    ISSN: 1432-0568
    Keywords: Dentinogenesis ; Dentinal globules ; Alkaline phosphatase ; Calcification ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary At the submicroscopic level, extracellular globular membrane-delimited structures have been observed in the matrix in early dentin formation in the rat. Within these “dentinal globules”, needle-like crystallites are found before apatite crystals appear in the filamentous-amorphous ground substance. In sections incubated for alkaline phosphatase, reaction products are closely associated with these globules. The possible role of the globules in initial dentin formation is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00519879
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  • 14
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    Electronic Resource
    A light microscope study of the cytology and organization of neurons in the simple mammalian nucleus lateralis: Columns and swirls (1973)
    Springer
    Anatomy and embryology 141 (1973), S. 125-150 
    Details
    ISSN: 1432-0568
    Keywords: Rat ; Columns ; Swirls ; Interneurons ; Projection neurons ; Subdivisions ; Morphology ; Golgi ; Nissl ; Axons ; Dendrites ; Cerebellum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The lateral nucleus in the rat is investigated with several variants of the rapid Golgi method and in Nissl preparations. The neurons are divided into two classes according to their size and the disposition of their axons. The smaller neurons or interneurons have cross sectional areas less than 180 μm2, and short axons that ramify in the vicinity of the cell bodies. Small neurons have also been seen on rare occasions with longer axons that may leave the nucleus. The larger cells (areas greater than 180 μm2) have long axons that leave the nucleus and emit short, beaded, recurrent collaterals. In the rostral and caudal poles of the lateral nucleus, the large projection neurons as well as the small neurons are multipolar with swirled dendritic arborizations. Neurons in the dorsal rim and ventral third of the nucleus have similar dispositions of their dendrites. In the central columnar zone, the projection neurons have dendrites that are arranged in columns directed diagonally across the body of the nucleus in the 4 to 10 o'clock direction in the right lateral nucleus and the 8 to 2 o'clock direction in the left nucleus. A collection of small neurons is generally found in the medial hilus zone. In each part of the lateral nucleus, the neurons are arranged in characteristic ways.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00519881
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  • 15
    Electronic Resource
    Electronic Resource
    Quantitative Beziehungen zwischen der Verzweigungsdichte und Länge von Capillaren im Neocortex der Ratte während der postnatalen Entwicklung (1973)
    Springer
    Anatomy and embryology 141 (1973), S. 207-221 
    Details
    ISSN: 1432-0568
    Keywords: Capillaries ; Endothelial cell ; Ontogenesis ; Morphometry ; Cerebral cortex ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Während der postnatalen Entwicklung wurde die Capillarlänge im Einheitsvolumen (CL), die Verzweigungsdichte (N⊥) und die mittlere Länge der Capillarendothelzellen (LE) in der Lamina IV des senso-motorischen Cortex von insgesamt 64 Rattengehirnen bestimmt. Als Capillaren wurden alle Gefäße unter 8 μm Durchmesser akzeptiert. Die CL bestimmten wir in 1–2 μm dicken Epon-Schnitten nach der Methode von Saltikov. Die Verzweigungen wurden durch Tusche-Gelatine-Injektion dargestellt und in Kryostat-Schnitten visuell gezählt. Im Verlauf des postnatalen Capillarwachstums, das die Bildung von Verzweigungen durch Sprossung und die Verlängerung der bestehenden Geäße umfaßt, nimmt die CL von ca. 200 mm auf 1100 mm pro mm3 Gewebe zu. Die Verzweigungszahl im gleichen Volumen steigt von 1200 kurz nach der Geburt auf ein Maximum von 11500 am Tag 20 und fällt dann allmählich auf den adulten Wert von 9500 ab. Am Ende der ersten Woche p.n. bewirkt die intensive Capillarsprossung eine starke Erhöhung der N⊥. Darauf folgt in der zweiten Woche p.n. die schnelle Zunahme der CL. Die Veränderungen der N⊥ laufen parallel mit Veränderungen im oxidativen Metabolismus. Aus dem Verhältnis N⊥ zu CL läßt sich der Vernetzungsgrad der Capillaren, d.h. die Verzweigungszahl pro mm Capillarlänge, berechnen. Dieser erreicht zwischen Tag 10 und 20 p.n. ein Maximum und sinkt danach bis zum erwachsenen Tier wieder beträchtlich ab. 1 Monat p.n. sind das Wachstum und die Reifung der Hirnrinde bei der Ratte weitgehend abgeschlossen. Die Bildung neuer Sprosse hört auf, und das weitere Kapillarwachstum erfolgt weniger durch Zellteilung als vielmehr durch Verlängerung des Endothels. Die Phase der intensiven Capillarsprossung entspricht zeitlich der vulnerablen Periode der Hirnentwicklung (“brain-growth-spurt”, nach Dobbing). Unsere Befunde lassen die Vermutung zu, daß in dieser Periode Störungen der Capillarsprossung indirekt das weitere Hirnwachstum beeinflussen können.
    Notes: Summary The average length of the capillary endothelial cells (L E ), the capillary length (CL) and the number of vascular ramifications (N⊥) per unit volume in layer IV of the sensorymortor cortex from the brains of 64 Sprague Dawley rats were determined during postnatal development. All vessels less than 8 μm in diameter were accepted as capillaries. The CL was calculated according to the method of Saltikov, by counting capillary profiles in 1–2μm Epon sections. The number of ramifications per test volume were counted in 30 μm frozen sections of India-ink injected brains. Capillary growth involves both the formation of ramifications by sprouting, and the elongation of the existing vessels. During the postnatal period CL increases from 200 to 1100 mm per cubic mm tissue, the extent of branching per unit volume rises from 1200 at birth to a peak of 11500 per cubic mm at day 20 after birth, and then declines to 9500 in adults. Intensive capillary sprouting results in a marked increase in N⊥ at the end of the first week, followed by a rapid increase in CL in the second week. The changes in the ramification density run parallel with the changes in aerobic metabolism during postnatal development. The ratio N⊥/CL, i. e. the number of ramifications per mm capillary length, corresponds to the degree of arborization of the capillary net. This value rises to a maximum level between days 10 and 20 after birth and declines until maturity is attained. After 1 month the growth of rat brain is almost accomplished and the cortical structure has attained its adult characteristics. The formation of new ramifications ceases and further capillary growth occurs to a larger extent by elongation of endothelial cells and not by cell division. The phase of extensive capillary sprouting corresponds to the vulnerable period of brain development (called “brain-growth-spurt” by Dobbing). Our findings lead to the assumption that disturbances during the period of capillary proliferation can indirectly influence further brain development
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    URL: http://dx.doi.org/10.1007/BF00519886
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  • 16
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    Electronic Resource
    Über glykoproteidhaltige amöboide Zellen im embryonalen Hühnergehirn (1973)
    Springer
    Anatomy and embryology 142 (1973), S. 341-358 
    Details
    ISSN: 1432-0568
    Keywords: Glycoproteid containing cells ; Microglia ; Brain development ; Chicken ; Light microscopy ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Zur Zeit der embryonalen und fetalen Entwicklung des Hühnchens treten vorübergehend runde, vacuolisierte, freie Zellen auf, die sich durch starken Gehalt und Glykoproteiden auszeichnen. Nachweis mit der Perjodsäure-Bisulfit-Aldehydthionin-Methode nach Specht (1970). Der Vergleich von Entwicklungsstadien zwischen dem 4. und dem 17. Bebrütungstag zeigt, daß die Zellen im Mesenchym entstehen und mit großer Wahrscheinlichkeit sowohl über das Ventrikelsystem als auch über die äußere Hirnoberfläche in das Gehirn einwandern. Die Zellen werden hauptsächlich in aufgelockerten, flüssigkeitsreichen Hirngebieten gefunden. Vermutlich geht die vorübergehende Auflockerung dieser Hirngebiete, die als Reservevolumen für nachfolgende histogenetische Wachstumsprozesse (Axonwachstum, Myelinisierung) verstanden wird, auf die Träger der PBA-positiven Zellen zurück (Ausschleusung von Glykoproteiden aus den PBA-positiven Zellen). Im gleichen Zeitraum bilden auch Ependymzellen Glykoproteide.
    Notes: Summary Round, vacuolated amoeboid cells with a high content of glycoproteids appear temporarily during the embryonic development of the chicken's brain. The cells were identified by the periodic-acid-bisulfit-aldehydthionine-method (Specht, 1970). A comparison of the developmental stages (4.–17. day of incubation) of the cells show that they originate in the mesenchym and probably immigrate by way of the ventricles as well as from the outer brain surface. The cells are mainly found in brain areas containing large, loosely structured extracellular spaces. The large extracellular gaps of these areas are probably developed in response to glycoproteid secretion by PBA-positive cells, as a preparatory measure for subsequent histogenetic growth processes (axon growth with myelinisation). At the same time glycoproteids are also produced by ependymal cells.
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    URL: http://dx.doi.org/10.1007/BF00519136
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  • 17
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    Electronic Resource
    The ultrastructure of lamellated mechanoreceptors in the skin of reptiles (1973)
    Springer
    Anatomy and embryology 143 (1973), S. 81-94 
    Details
    ISSN: 1432-0568
    Keywords: Electron microscopy ; Mechanoreceptors ; Skin ; Reptiles
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Lamellierte Receptoren in der Haut von Reptilien werden licht- und elektronenmikroskopisch beschrieben. Rekonstruktionen aus Schnittserien haben gezeigt, daß sie 1. als freie lamellierte Endigungen, 2. als lamellierte eingekapselte Endigungen und 3. als lamellierte eingekapselte Endigungen mit Kapselraum vorkommen. Sie haben eine Spindelform und können bis zu 0,5 mm lang sein. Die innere Lamellierung des eingekapselten Receptors mit Kapselraum vom Kaiman zeigt strukturelle Ähnlichkeiten zur inneren Hülle des Vater-Pacinischen Körperchens. Das Lamellensystem der Receptoren von Natter und Waran zeigt Parallelen zum Bau des inner core des Herbstschen Körperchens der Vögel. Die lamellierten Receptoren bilden einen regelmäßigen Bestandteil der sensiblen Innervation von Tastpunkten der Reptilienhaut.
    Notes: Summary Lamellated receptors in the skin of reptiles were studied with the light and electron microscope. As revealed by reconstructions of serial sections, there are three types of lamellated receptors: 1. lamellated free endings, 2. lamellated encapsulated endings and 3. lamellated encapsulated endings with a “fluid-filled” capsule space (outer core). The spindle shaped endings have a length of up to 0.5 mm. In Caiman, the lamellae (inner core) of the encapsulated receptor with a capsule space have a structure similar to that of the inner core of the Vater-Pacinian corpuscle. The lamellation (inner core) of the terminals of Natrix and Varan shows similarities with the inner core of the Herbst corpuscle. Lamellated receptors usually form an element of the sensory innervation of touch papillae in the reptile skin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00519912
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  • 18
    Electronic Resource
    Electronic Resource
    Helical, striated rootlets in the midpiece of a teleost fish spermatozoon (1973)
    Springer
    Anatomy and embryology 140 (1973), S. 11-17 
    Details
    ISSN: 1432-0568
    Keywords: Electron microscopy ; Cross-striated fibers ; Rootlets ; Midpiece ; Fish spermatozoa
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In the 45 μ long midpiece of the spermatozoon of Pantodon buchholzi nine helical, cross-striated fibers run from one end to the other and parallel nine helical mitochondrial threads; both fibers and mitochondrial threads surrounding the axoneme. The fibers have a major period about 750–900 Å and are very similar to ciliary rootlets and other cross-striated structures in terms of which they are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00520714
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  • 19
    Electronic Resource
    Electronic Resource
    Ultrastructural studies on the ontogenesis of the caudal neurosecretory system in the roach, Leuciscus rutilus (1973)
    Springer
    Anatomy and embryology 139 (1973), S. 207-216 
    Details
    ISSN: 1432-0568
    Keywords: Caudal neurosecretory system ; Ontogenesis ; Electron microscopy ; Teleost fish
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The ontogenesis of the urophysial system (caudal neurosecretory system) in the roach (Leuciscus rutilus) was ultrastructurally analyzed. In newly hatched spawns the urophysial components are differentiated in the form of neurosecretory perikarya, axons and terminals and display the characteristics of a functional system although the neurohemal area is not developed. It is assumed that the system is active simultaneously with the preopticohypophysial system during ontogenesis. On the contrary, organogenesis of the urophysis is late, and only at the 14 mm stage do the neurosecretory axons first penetrate the meninx to participate in the formation of the organ. Assumed aminergic cell types associated with the urophysial system are differentiated at the time of hatching, thus indicating a functional relationship. A secretory ependyma releasing its granules into the central canal is described.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00523639
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  • 20
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    Electronic Resource
    Late prenatal ontogeny of central monoamine neurons in the rat: Fluorescence histochemical observations (1973)
    Springer
    Anatomy and embryology 140 (1973), S. 281-318 
    Details
    ISSN: 1432-0568
    Keywords: Central monoamine neurons ; Ontogeny ; Rat ; Fluorescence histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The development of all monoamine-containing neuron systems in the rat brain throughout the last seven days of gestation, i.e. from the 15th day to term, crown rump length (CRL) 16 mm to 42 mm, has been followed using the Falck-Hillarp method for fluorescence histochemistry. Serial sagittal, horizontal and transverse sections through whole brains of fetuses from MAO-inhibited mothers have been carefully analyzed by fluorescence microscopy, and drawings of almost all sections depicting specific fluorescence have been collected from 10 of the 16 analyzed brains. Thus, all the 5-hydroxytryptamine (5-HT), dopamine (DA) and noradrenaline (NA) neuron systems have been mapped, both regarding the cell groups, the formation of axon pathways and the development of terminal plexuses in the whole brain prenatally. Together with a previous report (Olson and Seiger, 1972a) the present data have made it possible to determine the common origin of groups A1–A3, A4–A7, A8–A10, B1–B3 and B4–B9, respectively (nomenclature according to Dahlström and Fuxe, 1964). These findings and an estimation of the number of visible fluorescent cells in all monoamine cell complexes throughout the prenatal period of development have been summarized in a flow-chart. In the full-term fetus all monoamine neurons and their axon pathways, except for the hypothalamic cell groups, are very well developed. Thus, the new neuroanatomical data presented, in all probability hold true also for the adult state. A comprehension of the innervation course of neostriatum by DA nerve terminals from the 14th day of gestation to adult hood is given.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00525058
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  • 21
    Electronic Resource
    Electronic Resource
    Uterine stromal morphology of the spayed, virgin rat when prepared with progesterone and oestrogen for implantation (1973)
    Springer
    Anatomy and embryology 141 (1973), S. 161-169 
    Details
    ISSN: 1432-0568
    Keywords: Rat ; Uterus ; Stroma ; Ultrastructure ; Sex steroids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The effect of progesterone and oestrogen on the central uterine stroma has been examined in order to analyze what changes occur when the uterus is hormonally prepared for implantation. Both progesterone and oestrogen, when given separately, cause nucleolar enlargement and growth of the granular endoplasmic reticulum. However, after progesterone treatment the nuclei are large and spherical whereas they are spindle-shaped after oestrogen treatment. When oestrogen is administered to the progesterone-treated rat little change occurs in the stromal morphology. There is little morphological support to the view that oestrogen acts on the stroma to increase endometrial sensitivity. Further, progesterone seems to stimulate the synthesis of RNA and of cellular protein only in the stromal cell while oestrogen has a similar effect in all cell types of the uterus.
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    URL: http://dx.doi.org/10.1007/BF00519883
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  • 22
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    Electronic Resource
    Ultrastructure of myotendinous junctions in Myxine and rat specializations between the plasma membrane and the lamina densa (1973)
    Springer
    Anatomy and embryology 142 (1973), S. 91-101 
    Details
    ISSN: 1432-0568
    Keywords: Myotendinous junction ; Myxine and rat ; Lamina densa ; Plasma membrane ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Myotendinous junctions in the parietal muscle of the Atlantic hagfish (Myxine glutinosa, L.) and in the diaphragm of rat were studied with the electron microscope. The ends of muscle fibers posses deep tube-like invaginations and also finger-like processes. Terminal I-filaments of the myofibrils attach to the plasma membrane of the invaginations. Collagen fibrils of the tendon attach to the external aspect of the lamina densa. The lamina densa and also collagen fibrils continue into the invaginations. Specializations are present in the zone between the plasma membrane and the lamina densa of the invaginations, being most conspicuous in Myxine. These consist of 60 Å wide spine-like or thread-like profiles 150 Å apart, connecting with both the outer leaflet of the plasma membrane and the lamina densa. Spatially, they form parallel circular ridges oriented transversely in stacks along the invaginations. Similar spine-like profiles are also discernible in the invaginations of rat muscle fibers. In rat, however, their spatial arrangement (ridges or fibrils) remains unknown. These specializations may serve to attach the lamina densa to the plasma membrane, and may be important for the transmission of force from muscle fibers to tendon.
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    URL: http://dx.doi.org/10.1007/BF00519878
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  • 23
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    Electronic Resource
    Morphogenesis of the myoneural junctions after immobilization of the muscle in the rat (1973)
    Springer
    Anatomy and embryology 143 (1973), S. 1-12 
    Details
    ISSN: 1432-0568
    Keywords: Neuromuscular junction ; Development ; Cholinesterases ; Muscle ; Immobilization ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Immobilization of the tibialis anterior muscle of the newborn rat was caused by amputation of the hind limb at the talocrural joint. Subsequent muscular atrophy was measured by weighing the muscle and the myoneural morphogenesis in the disused muscle was studied in light and electron microscope. The acetylcholinesterase (AChE; E.C.3.1.1.7) and nonspecific cholinesterase (Ns. ChE; E.C.3.1.1.8) activity at the myoneural junction was followed histochemically. It was observed that the myoneural morphogenesis proceeded normally in the immobilized and atrophied muscle. Cholinesterase activities at the developing myoneural junction were identical on both the immobilized and the contralateral control side. The myoneural morphogenesis may be solely controlled by the nerve since, at the light of the present observations, the abnormally diminished function of the muscle seems to have no effect on the myoneural development.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00519906
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  • 24
    Electronic Resource
    Electronic Resource
    S-100 protein in methyl- and ethylnitrosourea induced tumors of the rat nervous system (1973)
    Springer
    Acta neuropathologica 24 (1973), S. 287-303 
    Details
    ISSN: 1432-0533
    Keywords: S-100 Protein ; Experimental Neurogenic Tumors ; Nitrosourea ; Rat ; Complement Fixation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary S-100 protein, a soluble protein restricted to the nervous system, was measured by complement fixation in 51 neurogenic and non-neurogenic tumors produced by either methylnitrosourea or ethylnitrosourea in three different strains of rats. Nineteen of the 51 neurogenic tumors were neoplasms of the central nervous system (18 of the brain, 1 of the spinal cord). They were diagnosed morphologically as 5 mixed gliomas, 4 anaplastic gliomas, 4 glioependymomas, 1 ependymoma, 3 gliosarcomas, and 2 unclassified tumors. With the exception of one anaplastic glioma and one gliosarcoma, all other central nervous system tumors contained S-100 protein, ranging from 0.005–0.13% of the total 35000 g supernatant protein. S-100 protein was also demonstrated in 21 of the 22 tumors of the peripheral nervous system, originating from the trigeminal nerves, the spinal roots, and from peripheral nerves. The average S-100 protein content of these tumors was 0.2% (range 0.02–1.6%). A possible correlation between S-100 protein content and tumor differentiation must be evaluated. S-100 protein was detected in only one of 10 neoplasms morphologically classified as non-neurogenic (7 sarcomas, 2 carcinomas, and 1 hemangioendothelioma). On the basis of its S-100 protein content, one tumor was reclassified as a neurosarcoma. The sensitivity and the high degree of specificity of the S-100 protein assay makes it a useful biochemical tool for the identification of neurogenic tumors. The presence of S-100 protein must be considered as a definitive indication for neural cell participation in neoplastic growth.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00685585
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  • 25
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    Electronic Resource
    Ultrastructural study of neuronal cytoplasmic inclusions produced by hypocholesterolemic Drug, AY 9944 (1973)
    Springer
    Acta neuropathologica 26 (1973), S. 185-197 
    Details
    ISSN: 1432-0533
    Keywords: Neuronal Inclusions ; Cerebrum ; Cholesterol Inhibitor ; Rat ; Electron Microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Intraperitoneal injections of a hypocholesterolemic drug, AY9944 produced neuronal cytoplasmic inclusions in the brain of Wistar strain of rats. The inclusions were numerous and larger in size in the younger age groups but gradually decreased in number and size after 30 days of age. Only a few small inclusions were seen in rats of 35 days of age or older, in spite of continuous injection of the drug. Inclusions gradually decreased in number after discontinuation of the drug in a relatively younger age group. Injection of the drug in adult rats produced only a few small neuronal inclusions. Ultrastructurally, concentrical lamellar structures were the predominant ones in younger age groups but other types of inclusions were seen in older animals and those receiving lesser amounts of the drug or those which were examined after discontinuation of the drug. This observation suggests that morphologically different inclusions can be formed by basically the same treatment if there is any difference in the metabolic activity of the target cell or the amounts of the drug. Biochemical analysis of the sterols revealed a consistently high level of 7-dehydrocholesterol up to 69 days of age, when AY9944 was administered continuously, despite the fact that there was a decrease in the number and size of the abnormal neuronal inclusions.
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    URL: http://dx.doi.org/10.1007/BF00684428
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  • 26
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    Electronic Resource
    Cell kinetics and morphology of the growth plate in the normal and hypophysectomized rat (1973)
    Springer
    Calcified tissue international 13 (1973), S. 113-129 
    Details
    ISSN: 1432-0827
    Keywords: Kinetics ; Growth ; Epiphyses ; Hypophysectomy ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Description / Table of Contents: Résumé La largeur de la métaphyse tibiale, la zone indifférenciée, la zone sériée et les cellules en dégénerescence ont été observées chez des rats Sprague-Dawley normaux et hypophysectomisés. La production cellulaire de la métaphyse est déterminée sur la base de la croissance osseuse longitudinale déterminée par l'oxytétracycline et la taille des cellules en dégénérescence. La diminution de la croissance osseuse longitudinale, en fonction de l'augmentation de l'âge et après hypophysectomie, est due partiellement, à la diminution de production cellulaire et partiellement à une décroissance de la taille des cellules en dégénérescence dans la métaphyse. L'influence de la production cellulaire et de l'activité mitotique prédomine.
    Abstract: Zusammenfassung Die Breite der proximalen Wachstumsplatte der Tibia, deren undifferenzierter und säulenförmiger Zone und die Größe der nahe bei der Metaphyse auftretenden degenerativen Zellen wurden in normalen und hypophysektomierten Sprague-Dawley-Ratten bestimmt. Die Zellproduktion in der Wachstumsplatte wurde aus dem longitudinalen Knochenwachstum berechnet, welches mittels Oxytetracyclin und der Größe der degenerativen Zellen bestimmt wurde. Es wurde festgestellt, daß die Abnahme des longitudinalen Knochenwachstums bei zunehmendem Alter und nach Hypophysektomie zum Teil einem Rückgang in der Zellproduktion, zum Teil einer Verminderung der Größe der degenerativen Zellen in der Wachstumsplatte zuzuschreiben ist. Der Einfluß der Zellproduktion, und somit der mitotischen Aktivität, herrscht vor.
    Notes: Abstract The width of the proximal growth plate of the tibia, its undifferentiated and columnar zone and the size of the degenerative cell close to the metaphysis, were determined in normal and hypophysectomized Sprague-Dawley rats. The cell production in the growth plate was calculated from the longitudinal bone growth determined with oxytetracycline and the degenerative cell size. It was found that the decrease in longitudinal bone growth with increasing age and after hypophysectomy, is due partly to a decrease in cell production, and partly to a decrease in degenerative cell size in the growth plate. The influence of cell production and thus the mitotic activity predominates.
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    URL: http://dx.doi.org/10.1007/BF02015402
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  • 27
    Electronic Resource
    Electronic Resource
    Maturation postnatale du tractus optique chez le Rat: Identification et quantification de la névroglie sur radio-autographies (1973)
    Springer
    Experimental brain research 16 (1973), S. 466-475 
    Details
    ISSN: 1432-1106
    Keywords: Neuroglia ; Radio-autography ; Myelination ; Optic tract ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Resume Une étude, basée sur l'identification et la quantification histologique des cellules névrogliques, a été entreprise dans le tractus optique du Rat sur radio-autographies après injection de thymidine tritiée. Ce bilan a été effectué entre 2 jours et demi et 20 jours après la naissance. D'un point de vue qualitatif, trois catégories cellulaires peuvent être distinguées: les oligodendrocytes, euxmêmes subdivisés en trois classes (grands, moyens, petits), les astrocytes et d'autres varieties cellulaires parmi lesquelles la microglie. D'un point de vue quantitatif, les oligodendrocytes, en particulier les petits, sont largement majoritaires. En effet, sur toute la période examinée les oligodendrocytes représentent près de 81% de la population totale. Sur le plan radio-autographique, l'index de marquage prédomine dans la classe des moyens oligodendrocytes. Ces résultats sont discutés, notamment par rapport aux processus de myélinisation dont les phénomènes de gliose constituent le préambule direct.
    Notes: Summary A study based on the histological identification and quantifying of the neuroglia cells, was carried out in the optic tract of the Rat on radioautographs after injection of tritiated thymidine. The assessment was made two and a half days and twenty days after birth. Qualitatively speaking, three cellular categories can be distinguished: the oligodendrocytes, themselves divided into three sorts (big, medium., small) the astrocytes and other cellular varieties including the microglia. Quantitatively speaking the oligodendrocytes, in particular the small ones, are decidedly more numerous. In fact, over the whole period under consideration the oligodendrocytes represent nearly 81% of the total population. From the point of view of the radio-autography the labeling index predominates in the medium oligodendrocyte group. These results are discussed with reference in particular to the myelination process which is preceded directly by the phenomena of gliosis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00234473
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    Electronic Resource
    Differentiation of principal cells of the rat lateral geniculate body into two groups; fast and slow cells (1973)
    Springer
    Experimental brain research 17 (1973), S. 242-260 
    Details
    ISSN: 1432-1106
    Keywords: Past and slow principal cells ; Recurrent inhibition ; Lateral geniculate body ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In urethane-anesthetized albino rats studies were made on mass potentials and/or unit activities of the lateral geniculate body (LGB) in response to single shock stimulation of optic tract (OT) and visual cortex (VC). The previous finding that LGB mass responses to stimulation of the optic nerve comprise at least two postsynaptic waves (r1 and r2) with different peak times was confirmed by OT stimulation. It was found that the r1 wave had a lower threshold and a more rapid recovery process than the r2 wave. Unit responses of principal (P) cells to maximal OT stimulation consisted of short-latency, single spikes (initial spike, IS), followed by long-latency, grouped discharges (late discharge, LD) repeated more than twice (LD1, LDr2 and so forth). Spontaneous discharges were almost completely suppressed after IS and each LD, suggesting that following these evoked discharges P cells underwent a phase of depressed excitability. The latency histogram of IS based upon 212 P cells had two peaks well-corresponding in time to the peaks of the r1 and r2 waves of the mass response, respectively. P cells were divided into the fast and slow groups according to whether their ISs occurred earlier or later than the trough separating the r1 and r2 waves. Typically, the fast cells had lower thresholds than the slow cells. The duration of the post-excitatory depression following IS and LD1 were shorter in the fast cells than in the slow cells. This same was found true with regard to the duration of depressed excitability following the antidromic firing. Examining the response patterns to OT stimulation of weak intensities, one characteristic difference between the two groups of P cells was found; the fast cells showed the IS without the succeeding LDs whereas the slow cells showed the LDs without the preceding IS. A discussion was made on functional significance of the differentiation of P cells into two types.
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    URL: http://dx.doi.org/10.1007/BF00234664
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  • 29
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    Influence of various corticoids on the augmentation of “Gomori-positive” granules in the median eminence of the rat following adrenalectomy (1973)
    Springer
    Naunyn-Schmiedeberg's archives of pharmacology 280 (1973), S. 49-62 
    Details
    ISSN: 1432-1912
    Keywords: Corticotropin-Releasing Factor ; Corticoids ; Median Eminence ; Rat ; Histology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In 465 female Wistar rats the influence of an i.m. injection of various natural or synthetic corticoids on the augmentation of “Gomori-positive” granules in the outer layer of the median eminence following bilateral adrenalectomy has been investigated. While the augmentation of the granules is not impaired by progesterone it is inhibited or completely blocked, depending on the dose and the manner of application, by 11-desoxycorticosterone, corticosterone, cortisol, 9α-fluorocortisol, prednisolone, triamcinolone, dexamethasone and fluoroformylone. The doses necessary to suppress the augmentation of the granules correspond to those shown by other authors to be required for blocking ACTH-secretion. The inhibitory effect of the investigated corticoids on the augmentation of the granules is related to their antiphlogistic potency. The findings suggest that the amount of the “Gomori-positive” granules in the outer layer of the median eminence of the rat might be used as a parameter of the activity of the corticotropin-releasing factor.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00505354
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  • 30
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    Electronic Resource
    Intragastric self-administration of medazepam in rats (1973)
    Springer
    Psychopharmacology 28 (1973), S. 87-94 
    Details
    ISSN: 1432-2072
    Keywords: Psychopharmacology ; Intragastric ; Self-Administration ; Benzodiazepines ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract A technique for intragastric self-administration in rats is presented. Intravenous self-administration of morphine was compared with intragstric self-administration during 6 consecutive days. There was no difference between the two routes in the total rate of injections. Medazepam was given for intragastric self-administration at three different doses (2.5, 5.0 and 10.0 mg/kg/injection respectively) and compared with saline. There was an increased response rate at the dose 10 mg/kg/injection. Medazepam placebo solution (solvent alone) was also compared with medazepam 10 mg/kg/injection. Among the seven experimental rats, six responded higher on medazepam than on the solvent.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00413960
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  • 31
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    Acute effects of two tetrahydrocannabinols (Δ9-THC and Δ8-THC) on water intake in water deprived rats: Implications for behavioral studies on marijuana compounds (1973)
    Springer
    Psychopharmacology 30 (1973), S. 315-322 
    Details
    ISSN: 1432-2072
    Keywords: Δ 9-THC and Δ 8-THC ; Water Intake ; Food Intake ; Body Weight ; Vocalization ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Water intake was studied in water deprived albino rats at various time intervals after injections of two tetrahydrocannabinols (Δ 9-THC and Δ 8-THC) and solvents. The dose levels used were: 1.25, 2.5, and 5.0 mg/kg of Δ 9-THC and 2.5, 5.0, and 10.0 mg/kg of Δ 8-THC. The results show a clear, dose dependent inhibitory effect on water intake as compared to the controls. Reduced intake of food was seen at 1 day post injection. This effect was, however, significant only for the groups treated with 5.0 and 10.0 mg/kg of Δ 8-THC. A decreased body weight was also recorded after the drug treatment, especially with Δ 8-THC. With respect to cannabis-induced vocalization the data suggest an increased possibility of its appearance with increasing dosages of THC.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00429190
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  • 32
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    Effects of methamphetamine and chlordiazepoxide on schedule-controlled and adjunctive licking in the rat (1973)
    Springer
    Psychopharmacology 30 (1973), S. 375-384 
    Details
    ISSN: 1432-2072
    Keywords: Schedule-Controlled Behavior ; Adjunctive Behavior ; Licking ; Drinking ; Methamphetamine ; Chlordiazepoxide ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The effects of methamphetamine (0.03–1.7 mg/kg) and chlordiazepoxide (3.0–30.0 mg/kg) were studied in a situation in which the same motor response, licking a water-filled tube, served as a schedule-controlled and as an adjunctive behavior. Rats responded under a 3-min fixed-interval (FI) schedule of food presentation in which the required response was a lick on the tube (schedule-controlled); licking also occurred following every food presentation (adjunctive). Adjunctive licking occurred at a high, steady rate, but schedule-controlled licking was emitted at a changing rate over time, characteristic of FI schedules. Both drugs had little effect on overall adjunctive licking, except for decreases at the highest doses, but there were changes in the pattern of adjunctive licking. Methamphetamine produced only decreases in schedule-controlled licking, but chlordiazepoxide produced dose-dependent increases. In general, the magnitude of drug effect on local rates of responding within the FI was related to control rates of responding within the same periods, but there were instances in which the magnitude of effect depended also on whether licking was adjunctive or schedule-controlled.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00429197
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  • 33
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    Dissociation de deux composantes du comportement chez le rat sous l'effet de psychotropes (1973)
    Springer
    Psychopharmacology 31 (1973), S. 77-90 
    Details
    ISSN: 1432-2072
    Keywords: Rearing ; Locomotion ; Exploratory Behaviour ; Psychotropic Drugs ; Anxiolytics ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The “staircase” is a simple and rapid test and was used to study two components of exploratory behaviour in the rat. The scores of rearing and the number of steps climbed during three minutes were recorded. Various psychotropic drugs were tested, which modified these two parameters. Neuroleptic induced a parallel decrease of both, while benzodiazepines, meprobamate, amobarbitone and ethanol decreased the rearing at doses which left the steps climbed unchanged. At high doses, there was a parallel decrease of both parameters. Amphetamine, at lower doses, increased the rearing score alone. The comparison of the studied psychotropic drug effects with those of two muscle relaxants (by a comparison of the slopes of regression lines) suggests that either the observed benzodiazepine effects were only partly due to their myorelaxant action, or, that both myorelaxants have some anxiolytic action. The effect of amphetamine at low doses can be viewed as a demonstration of increased anxiety.
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    URL: http://dx.doi.org/10.1007/BF00429300
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  • 34
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    Δ9-tetrahydrocannabinol induced inhibition of predatory aggression in the rat (1973)
    Springer
    Psychopharmacology 31 (1973), S. 157-166 
    Details
    ISSN: 1432-2072
    Keywords: Δ 9-tetrahydrocannabinol ; Predatory Aggression ; Rat ; Serotonin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Δ 9-tetrahydrocannabinol inhibits predatory aggression in rats. Increases in the degree of inhibition are obtained with increasing dosages of the drug. Although sex and strain of the subjects varied nonsystematically, inhibition of predatory aggression uniformly was found to be dose related. Readministration of Δ 9-tetrahydrocannabinol did not effect motor activity measures at dose levels which inhibit aggression. Administration of Δ 9-tetrahydrocannabinol increases whole brain serotonin, while norepinepherine is largely unaffected, indicating that the anti-aggressive effect may be mediated, in part, by serotonergic mechanisms.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00419815
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  • 35
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    Behavioural effects of yohimbine administered intraventricularly in the rat (1973)
    Springer
    Psychopharmacology 33 (1973), S. 267-275 
    Details
    ISSN: 1432-2072
    Keywords: Yohimbine ; Intraventricular Administration ; Rat ; Locomotor Activity ; Temperature ; Narcosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The influence of yohimbine injected into the lateral cerebral ventricle on the behaviour of white Wistar rats was investigated. Yohimbine in low doses (1–5 Μg) produced excitatory effects but in high doses (20–100 Μg) it induced central depression.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00423061
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  • 36
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    Drinking behaviour and brain dopamine: Antagonistic effect of two neuroleptic drugs (Pimozide and Spiramide) upon amphetamine- or apomorphine-induced hypodipsia (1973)
    Springer
    Psychopharmacology 33 (1973), S. 299-308 
    Details
    ISSN: 1432-2072
    Keywords: Amphetamine ; Apomorphine ; Neuroleptics ; Dopamine ; Drinking Behaviour ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Hypodipsia produced by injection of d-amphetamine (2.0 mg/kg) or apomorphine (0.8 mg/kg) in rats, was partially antagonized by two DA-specific neuroleptic drugs, Pimozide and Spiramide, respectively. Pimozide revealed a maximal amphetamine-antagonistic effect at dose levels between 0.1–0.4 mg/kg. Hypodipsia could also be produced by Pimozide alone in doses greater than 1.0 mg/kg. Pretreatment of the apomorphine-induced hypodipsia with 0.05 mg/kg Spiramide also reliably counteracted drinking deficits. The interaction of water deprivation combined with the presence or absence of food in the test situation was also examined, but no effect was found. The possibility that perseverative rearing on the hind legs under d-amphetamine might interfere with drinking was tested with high vs. low drinking-tubes in the Pimozide-amphetamine experiments. There was evidence for a slight initial effect of drinking position, but the general form of the dose-response curve was not greatly altered. It was concluded that dopamine effects cannot easily be excluded from a role in the control of drinking, and that the primary role often accorded norepinephrine in relation to amphetamine effects should be re-examined with respect to the specific behavioural functions which are altered.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00437507
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  • 37
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    Effects of a gastro-secretory substance from hog pancreas (GSP) and gastrin on bile secretion of the rat (1973)
    Springer
    Pflügers Archiv 345 (1973), S. 73-79 
    Details
    ISSN: 1432-2013
    Keywords: Gastrosecretagogue ; Gastrin ; Rat ; Bile
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The effect of GSP, a gastrosecretagogue fraction extracted from hog pancreas, on bile secretion has been studied in rats provided with a bile duct canula and compared with the effect of antral gastrin. GSP showed no effect on bile secretion, concentration and output of bilirubin and electrolytes in the bile. On the other hand gastrin is significantly increasing bile output, bilirubin concentration and output in the bile. No effect of gastrin on the excretion rate of sodium and potassium in the bile has been observed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00587063
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  • 38
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    Hypoxic pulmonary steady-state diffusing capacity for CO and cardiac output in rats born at a simulated altitude of 3500 m (1973)
    Springer
    Pflügers Archiv 340 (1973), S. 11-18 
    Details
    ISSN: 1432-2013
    Keywords: Pulmonary Diffusing Capacity for CO ; Cardiac Output ; Oxygen Dissociation Curve ; High Altitude ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In rats born in the low pressure chamber from sea level parents a higher hypoxic steady-state pulmonary diffusing capacity for CO was found as compared with controls of similar body weight. This difference could be explained by a difference in age or by an increase of blood O2 capacity. There was no difference in alveolar ventilation and alveolar-arterial O2 pressure differences, a lower cardiac output, no difference in arterial O2 tension, no difference in arterial O2 content but a decreased mixed-venous O2 content as compared with control rats measured at hypoxia. A shift of the standard blood O2 dissociation curve to the right was found in the simulated high altitude exposed rats. Calculated mixed-venous O2 pressure was not altered in these rats; since arterial O2 pressure was the same no difference in mean tissue capillary O2 pressure may be presumed as compared with control animals. The results suggest that the first generation of rats exposed to simulated high altitude for their whole life is not only less adapted than animals exposed in their youth (as described in previous work) but that the ability to promote the O2 transport in time of need in rats born in the low pressure chamber is probably even inferior to that of the controls.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00592193
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  • 39
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    Myoglobin distribution in the heart of growing rats exposed to a simulated altitude of 3500 m in their youth or born in the low pressure chamber (1973)
    Springer
    Pflügers Archiv 340 (1973), S. 1-10 
    Details
    ISSN: 1432-2013
    Keywords: Myoglobin ; Heart ; High Altitude ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Reynafarje's (1963) spectrophotometric method was modified for the study of myoglobin concentration in rat hearts. The influence of age and body weight was evaluated and the age was found to be better correlated with myoglobin concentration than the weight. The influence of simulated altitude of 3500 m was studied in animals exposed to hypoxia at the age of about 34 days and in animals born in the low pressure chamber. In the first group a higher myoglobin concentration was found but only in the right ventricle, together with unilateral hypertrophy, as compared with control animals. In the group of animals born at simulated altitude a severe cardiomegaly developed which was most pronounced in the right ventricle. The myoglobin concentration in the hearts from this group was unchanged in the right ventricle and was lower in the left ventricle and septum.
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    URL: http://dx.doi.org/10.1007/BF00592192
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  • 40
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    Demonstration of nigro-neostriatal tract by degeneration silver method (1973)
    Springer
    Experimental brain research 17 (1973), S. 133-138 
    Details
    ISSN: 1432-1106
    Keywords: Nigro-neostriatal tract ; Rat ; Survival time ; Fink-Heimer method ; Terminal degeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In the morphological demonstration of the nigro-neostriatal tract, there might be some discrepancies between the results by the histofluorescence method and by the degeneration silver method. The present investigation was undertaken to fill the gap by using the modified Nauta Method (Fink-Heimer) to rats surviving for varying periods after unilateral destruction of the substantia nigra. With survival time of 2–3 days, the Fink-Heimer method could demonstrate evenly and densely distributed argyrophilic spheroidal granules in the ipsilateral caudate putamen. These degenerating granules might represent the terminal degeneration of the nigro-neostriatal tract and probably correspond to the result by the histofluorescence method. From 4 days on the terminal degeneration rapidly decreased in amounts or disappeared, leaving a moderate number of beaded coarse fibers, which remained till 2 weeks. The result indicates the possibilities that the degeneration silver method could also be applicable to the demonstration of amine fibers by choosing staining condition and survival time optimal to each animal species and fiber tract.
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    URL: http://dx.doi.org/10.1007/BF00235022
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  • 41
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    Electronic Resource
    Electrotonic coupling between neurons in the rat lateral vestibular nucleus (1973)
    Springer
    Experimental brain research 16 (1973), S. 255-275 
    Details
    ISSN: 1432-1106
    Keywords: Rat ; Lateral vestibular nucleus ; Giant cells ; Gap junctions ; Electrotonic coupling
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Correlation of morphological and electrophysiological data strongly suggest that in rat, the giant cells of the lateral vestibular nucleus (L.V.N.) are electrotonically coupled. 1. in addition to “active zones” large terminals synapsing on the perikaryon and/or the main dendritic trunk of the cells bear “gap” junctions which are interpreted as low electrical resistance pathways between neurons. 2. electrical activity of the giant cells was recorded intracellularly as the vestibulo-spinal tract was stimulated. Graded antidromic stimulation produced graded antidromic depolarizations (G.A.Ds) in 69% of cells with high threshold axons. 3. the latency of the G.A.Ds was too short to allow for chemical transmission through afferents or recurrent collaterals. 4. collision experiments demonstrated that directly evoked spikes blocked the antidromic spikes but did not block the G.A.Ds which thus were accounted for by activation of cells others than the impaled ones. 5. lesion experiments indicated that afferent fibers from the spinal cord terminate exclusively in the dorsal part of the L.V.N. Since G.A.Ds were recorded all throughout the nucleus, they were not excitatory post synaptic potentials (EPSPs) from spinal afferents. 6. when the strength of the spinal cord stimulation was increased EPSPs were also generated but they were distinct from the G.A.Ds by their latencies, time course and maximum amplitude. 7. since no direct contact is observed between neurons it is inferred that, as in other documented cases, coupling between giant cells is mediated by way of presynaptic fibers.
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    URL: http://dx.doi.org/10.1007/BF00233330
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  • 42
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    Normal plasma growth hormone levels in growth-retarded weanling rats with dorsomedial hypothalamic lesions (1973)
    Springer
    Experimental brain research 18 (1973), S. 374-382 
    Details
    ISSN: 1432-1106
    Keywords: Growth retardation ; Hypothalamus ; Dorsomedial lesions ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Weanling rats received bilateral electrolytic lesions destroying the areas of the ventromedial (VMN) and dorsomedial (DMN) hypothalamic nuclei, respectively. Trunk blood was collected at sacrifice (Experiment 1 and 2∶ 31 days, Experiment 3∶ 14 days) for the determination of plasma growth hormone (GH) by radioimmunoassay. Rats with DMN lesions showed consistently normal to slightly elevated plasma GH levels while animals with VMN lesions showed significantly reduced plasma GH levels. Linear growth was reduced by both types of hypothalamic lesions but body weight gains and food intake were reduced only in the DMN lesioned rats. Carcass fat was greatly elevated in VMN lesioned rats, moderately elevated in one experiment in the DMN animals and normal in another experiment. These data confirm a previous hypothesis that growth retardation in DMN rats is not due to low circulating GH level but rather represents a hypocaloric-type dwarfism.
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    URL: http://dx.doi.org/10.1007/BF00239106
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  • 43
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    Arzneimittelbedingte Hyposiderämie bei ratten (1973)
    Springer
    Archives of toxicology 30 (1973), S. 187-198 
    Details
    ISSN: 1432-0738
    Keywords: Plasma Iron Level ; Side Effect of Drugs ; Rat ; Amphetamine ; Chlorpromazin ; Haloperidolum ; Guanethidin ; Reserpine ; α-Methyltyrosine. ; Plasmaeisenspiegel ; Arzneimittelnebenwirkungen ; Ratte ; Amphetamin ; Chlorpromazin ; Raloperidol ; Guanethidin ; Reserpin ; α-Methyltyrosin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung 4 Std nach einmaliger Gabe von 24 mg/kg d,l-Amphetamin i.p. kommt es bei Ratten zu einem Abfall des Eisens im Plasma um 40 bis 60 %. Die Reaktion ist nicht mit dem amphetaminbedingten Anstieg von Körpertemperatur und Skeletmuskelarbeit zu erklären, da sie durch Vorbehandlung mit α-Methyltyrosin, Chlorpromazin, Haloperidol, Guanethidin und Reserpin nicht unterdrückt werden kann. Jedes der zur Vorbehandlung verwandten Medikamente löst selbst bei entsprechend hoher Dosierung eine Hyposiderämie aus. 4 Std nach einmaliger Gabe von 200 mg/kg α-Methyltyrosin vermindert sich der Eisenspiegel im Plasma um 55 %, nach 10 mg/kg Chlorpromazin um 11 %, nach 5 mg/kg Haloperidol um 28 % und nach 20 mg/kg Guanethidin um 50 %. Dieser Effekt ist am ausgeprägtesten mit einer Plasmaeisenverminderung um 69 % 20 Std nach Gabe von 20 mg/kg Reserpin. Als möglicher Pathomechanismus der arzneimittelbedingten Hyposiderämie wird die Wirkung der untersuchten sämtlich zentralnervös aktiven Medikamente als “non-specific stressfull agents” sowie der durch ihre toxische Konzentration in Gang gesetzte Entgiftungsmechanismus im RES diskutiert.
    Notes: Abstract Four hours following a single dose of 25 mg/kg of d, 1-Amphetamine in rats, a 40 to 60 % drop of plasma iron level occurred. This reaction cannot be explained by the Amphetamine-induced rise in body temperature and motor activity, since when pretreated with α-Methyltyrosine, Chlorpromazin, Haloperidolum, Guanethidin and Reserpin this was not observable. All the drugs applied for pretreatment in adequately high doses also induced hyposideremia. Four hours following a single dose of 200 mg/kg of α-Methyltyrosine, plasma iron level was reduced by 55 %, after 10 mg/kg of Chlorpromazin by 11 %, after 5 mg/kg of Haloperidolum by 28 % and after 20 mg/kg of Guanethidin by 50 %. The most pronounced effect (69 % fall) was obtained by 20 hour pretreatment with 20 mg/kg of Reserpin. As possible pathomechanism of the drug induced hyposideremia the applied drugs may be regarded as “non-specific stressfull agents”, or as another possibility, the detoxifying mechanism in RES induced by toxic concentrations of the applied drugs can be considered as responsible for this phenomena.
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    URL: http://dx.doi.org/10.1007/BF02426042
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  • 44
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    Schwerer Arzneimittel-Ikterus durch das Tuberculostaticum Isonicotinsäurehydrazid (INH) bei einem Kleinkind mit Phenylketonurie (1973)
    Springer
    European journal of pediatrics 115 (1973), S. 49-58 
    Details
    ISSN: 1432-1076
    Keywords: Phenylketonuria ; Seizures ; Isoniazid ; Jaundice ; Electron microscopy ; Degeneration of organelles in liver cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Bei einem jordanischen Kleinkind, das wegen einer Phenylketonurie mit cerebralen Krampfanfällen seit 1 Jahr mit Primidon und Ospolot behandelt und seit 7 Monaten mit einer phenylalaninarmen Diät unter Zusatz eines Aminosäurengemisches ernährt wurde, entwickelte sich nach 3 monatiger INH-Medikation ein hepatocellulärer Arzneimittelikterus. Dieser erwies sich klinisch und histologisch als reine Form einer toxischen Hepatose ohne entzündliche Symptome. Die elektronemmikroskopische Untersuchung von Leberbiopsiematerial 2 Wochen nach dem Höhepunkt des Ikterus (28 mg% Gesamtbilirubin im Serum) erlaubte den Nachweis eines intracellulären Transportdefektes des bereits konjugierten Bilirubins in den Leberepithelien. Das Pigment wurde analog dem Dubin-Johnson-Syndrom in peribiliären Lysosomen gespeichert. Weitere für die Pathogenese aufschlußreiche Befunde wurden an den Organellen der Hepatocyten erhoben: Hydropische Schwellung des hypertrophierten glatten endoplasmatischen Reticulums bei gleichzeitiger Reduktion des rauhen endoplasmatischen Reticulums, Glykogenschwund und Degeneration der Mitochondrien. Bei der Kontrollbiopsie nach 1/2 Jahr waren alle Befunde bis auf die lysosomale Speicherung normalisiert.
    Notes: Abstract After being treated with isonicotinic acid hydrazine (INH) for 3 months a 3-year-old Jordanian boy with phenylketonuria developed severe hepatocellular jaundice. In addition to INH the patient had received Primidone and Ospolot® for 1 year and a PKU diet for 7 months without any side effects. The liver biopsy 2 weeks after the peak of jaundice revealed no signs of hepatitis on light microscopy. An electron microscope investigation showed a defect in the intracellular transport system of bilirubin; bile pigment was stored in peribiliar lysosomes in the same way as in the Dubin-Johnson Syndrome. The smooth endoplasmatic reticulum was increased and vacuolized, whereas rough endoplasmatic reticulum, mitochondria, and glycogen were reduced. In a control biopsy nearly 6 months after the jaundice the morphologic alterations of the liver had normalized with the exception of the lysosomal changes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00438990
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    The effects of fenfluramine on fixed ratio responding (1973)
    Springer
    Psychopharmacology 32 (1973), S. 351-358 
    Details
    ISSN: 1432-2072
    Keywords: Fenfluramine ; FR Responding ; Behavioural Tolerance ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Two experiments on the effects of fenfluramine on FR responding are reported. In Experiment 1 two rats were trained on an FR 10 schedule of reinforcement. After more than 30 h training, intraperitoneal injection of saline, 2, 4 and 8 mg/kg fenfluramine were given. Disruption of the initial stages of responding was noted after 2 and 4 mg/kg, with complete disruption of responding in 1 animal after 8 mg/kg administration. In Experiment 2, 2 rats trained on an FR 30 schedule of reinforcement received chronic 15 days administration of 4 mg/kg fenfluramine. Disruption of responding was noted after the first administration. The degree of disruption lessened by the fifteenth day of administration.
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    URL: http://dx.doi.org/10.1007/BF00429471
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  • 46
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    Electronic Resource
    Effects of lithium chloride on the activity of rats tested alone or in pairs (1973)
    Springer
    Psychopharmacology 29 (1973), S. 85-89 
    Details
    ISSN: 1432-2072
    Keywords: Lithium ; Social Isolation ; Spontaneous Activity ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Group-housed rats, injected with an acute dosage of 3 meq/kg lithium chloride, were tested on an activity platform either alone or in pairs 20 min or 3 h after drug administration. Contrary to previous findings, rats administered lithium were less active than those treated with saline after both post-injection periods. However the activity of the saline control animals tested alone after 3 h was considerably less than that exhibited by the same group after 20 min. This activity difference, which could be interpreted as a lithium-social or lithium-time interaction, is more simply explained in terms of the effects of the social isolation occuring between drug administration and testing.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00421214
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  • 47
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    Electronic Resource
    Conditioned aversion to a preferred solution following methamphetamine injections (1973)
    Springer
    Psychopharmacology 29 (1973), S. 253-261 
    Details
    ISSN: 1432-2072
    Keywords: Methamphetamine ; Rat ; Conditioned Aversion ; Saccharin Solution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Holtzman (Sprague-Dawley) rats which were injected in amounts ranging from 0.50–3.0 mg/kg i.p. of methamphetamine avoided a 0.1% saccharin solution which had been paired temporally with the injection, and drank water in a subsequent two-bottle choice situation under non-drug conditions. The aversion persisted for the duration of the experiment, which lasted and obtained whether the animals were fluid-deprived or fluid-satiated. Saline-injected controls, on the other hand, exhibited a 90% saccharin solution preference. Rats which received 0.25 mg/kg contiguous with the drug, or animals given 3.0 mg/kg paired with water, exhibited as extreme a preference for the saccharin solution as did the saline controls. It was concluded that neither a drugmediated taste quality change nor physiological cues associated with thirst were adequate axplanations for the avoidance behavior exhibited, but that the drug was perceived as a noxious stimulus at levels above 0.50 mg/kg under the conditions described above.
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    URL: http://dx.doi.org/10.1007/BF00414040
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  • 48
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    Electronic Resource
    The role of catecholamines in behavioral arousal during ontogenesis (1973)
    Springer
    Psychopharmacology 31 (1973), S. 253-264 
    Details
    ISSN: 1432-2072
    Keywords: Locomotor Activity ; α-Methyl-Para-Tyrosine ; Reserpine ; l-Dopa ; Ontogenesis ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Effect of catecholamine depletion on normal hyperactivity in the neonatal rat was examined. Both α-methyl-para-tyrosine and reserpine significantly depressed behavioral arousal at 15 days postpartum, the age of greatest excitability. Heightened activity could be restored in drug-treated animals by administration of l-Dopa. These results indicate that the ontogenetic hyperactivity effect is a result of accelerated catecholamine function.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00422515
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  • 49
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    Electronic Resource
    Nonlinear changes in activity and emotional reactivity scores following central noradrenergic lesions in rats (1973)
    Springer
    Psychopharmacology 32 (1973), S. 313-325 
    Details
    ISSN: 1432-2072
    Keywords: 6-Hydroxydopamine ; Catecholamine Hypothesis of Depression ; Animal Model ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Two levels of permanent central norepinephrine depletion were obtained in rats by lesioning these neurons with small quantities of intraventricularly-applied 6-hydroxydopamine, and the effects of these lesions on a variety of standard tests of activity and emotional reactivity were studied. Both lesioned groups showed altered emotional reactivity; however, only the animals with less extensive lesions were hyperactive in running wheels, showed heightened shock-elicited aggression, were hyper-responsive to handling, and had a potentiated amphetamine-activation effect. It is suggested that these exaggerated behaviors reflected the sensitization and regeneration of partially lesioned noradrenergic circuits. These data are discussed in terms of the catecholamine hypothesis of depression.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00429467
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  • 50
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    Electronic Resource
    Effects of Δ1(2)-tetrahydrocannabinol on copulation in the male rat (1973)
    Springer
    Psychopharmacology 28 (1973), S. 243-246 
    Details
    ISSN: 1432-2072
    Keywords: δ 1-THC ; Copulation ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The mating behavior of 15 male rats was measured after treatment with δ 1(2)-tetrahydrocannabinol (THC) and propylene glycol (vehicle). Administration of either 2 mg per kg or 3 mg per kg body weight of THC was followed by significant increases in latency to the first mount, latency to ejaculation, and latency to the first mount following ejaculation. No significant changes were found in the number of intromissions or mounts. The deterioration in sexual performance is interpreted as reflecting a decreased motivation to copulate under the influence of the drug.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00429304
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  • 51
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    The influence of blood flow on the absorption of 3-O-methylglucose from the jejunum of the rat (1973)
    Springer
    Naunyn-Schmiedeberg's archives of pharmacology 279 (1973), S. 153-172 
    Details
    ISSN: 1432-1912
    Keywords: Intestinal Blood Flow ; Intestinal Absorption ; 3-O-Methylglucose ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary 1. Jejunal loops of anaesthetized rats were perfused (single pass perfusion and recirculation) with buffered solutions containing 3-O-methylglucose (3-OMG) in two concentrations: below (about 30 μM) and above (about 30 mM) blood glucose concentration. The blood flow, the appearance rate (in the intestinal venous blood) and the disappearance rate (from the intestinal lumen) were determined simultaneously, the accumulation in the gut wall at the end of the experiments. 2. At constant intermediate blood flow the appearance rate and disappearance rate decreased with time. A decrease of the blood flow rate from about 1.8 to about 0.3 ml min−1g−1 diminished the appearance and disappearance rate. An increase of blood flow from about 0.3 to about 1.9 ml min−1g−1 caused no increase of absorption; even a slight decrease was observed. 3. A kinetic analysis of the data showed that the different blood flow dependence of 3-OMG absorption in the experiments with increasing and decreasing blood flow can be explained sufficiently by a decrease of transport capacity with time, presumably due to an altered regional blood flow (decreased villous blood flow rate, unchanged total rate). A postischemic inhibition of 3-OMG absorption was of less importance. 4. The increase in perfusion rate from 0.1 (single pass perfusion) to 10 ml min−1 (recirculation) caused an increase of 3-OMG absorption and tissue accumulation.
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    Electronic Resource
    The effect of brocresine (NSD-1055) on diamine oxidase activity in plasma, liver, stomach and upper small intestine of rats (1973)
    Springer
    Naunyn-Schmiedeberg's archives of pharmacology 278 (1973), S. 425-430 
    Details
    ISSN: 1432-1912
    Keywords: Brocresine ; Diamine Oxidase ; Rat ; Plasma ; Liver ; Stomach ; Small Intestine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary 1. In male rats DAO was found to be absent in plasma and liver, very low in gastric and high in the upper small intestinal mucosa. 2. Gastric mucosal DAO was completely abolished by 10−8 M Brocresine. In the small intestine inhibition of DAO by Brocresine was dose-dependent, with a concentration of 6.2×10−6 M producing a 50% inhibition of the enzyme. 3. Pretreatment of rats with 100 mg/kg Brocresine i.p. produced a 43% inhibition of DAO after 1 h and an insignificant inhibition by 20% after 4 h. 4. The results suggest that the histidine decarboxylase inhibitor Brocresine is not a very suitable tool for studying the physiological role of histamine by inhibiting its biosynthesis since it also interferes with the histamine catabolism.
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    URL: http://dx.doi.org/10.1007/BF00501484
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    Electronic Resource
    A morphologic and histochemical study of biliary atresia in lamprey liver (1973)
    Springer
    Cell & tissue research 136 (1973), S. 85-96 
    Details
    ISSN: 1432-0878
    Keywords: Liver ; Lampetra ; Physiological biliary atresia ; Electron microscopy ; Histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A morphologic and histochemical study was carried out on the liver of larval and adult lampreys at the optical and electron microscopic level. In the larva the liver is composed of blind ending single cell thick tubules of hepatocytes. The tubular lumina provided with microvilli are morphologically comparable with the canalicular lumens of the higher species of animals. The cytoplasm of the hepatocytes contains numerous inclusions with heterogeneous appearance and crystalline material. The biliary system is composed of numerous bile ductules and ducts. In the adult lamprey, the biliary system has disappeared. The hepatocytes loose their tubular arrangement and the characteristic differentiation of their biliary pole. In contrast to previous reports in the literature the presence of bile pigment in the adult lamprey liver could not be demonstrated with any histochemical technique.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307681
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  • 54
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    Electronic Resource
    Elektronenmikroskopische Untersuchungen zur embryonalen und postembryonalen Genese der Eierstockzwischenzellen des Haushuhns (1973)
    Springer
    Cell & tissue research 136 (1973), S. 59-83 
    Details
    ISSN: 1432-0878
    Keywords: Interstitial cells, genesis ; Hen's ovary ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Für die Zwischenzellen des Hühnerovars gibt es embryonal und post-embryonal bindegewebige und epitheliale Quellen. Die bindegewebigen Mutterzellen sind Mesenchymzellen und Fibrozyten, die epithelialen Mutterzellen sind Wandepithelzellen der Markstranglakunen und Epoophorontubuli. Kriterien zur Unterscheidung dieser vierfachen Abstammungsmöglichkeiten der Zwischenzellen werden angegeben.
    Notes: Summary The interstitial cells of the hen's ovary have two sources: connective tissue mother cells, which are mesenchymal cells or fibrocytes, and epithelial mother cells, which are wall epithelial cells of the medullary cord lacunae or of the tubuli of the epophoron. Criteria are provided for differentiating this fourfold genesis of the interstitial cells.
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    URL: http://dx.doi.org/10.1007/BF00307680
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  • 55
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    Electronic Resource
    Elektronenmikroskopische Untersuchungen zur funktionellen Morphologie des Tubero-hypophysären Systems der Ratte (1973)
    Springer
    Cell & tissue research 139 (1973), S. 101-148 
    Details
    ISSN: 1432-0878
    Keywords: Infundibulum ; Rat ; Neuro-glial synapses ; Neuro-Vascular contacts ; Corticotropin-Releasing Factor ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung 1. Die Gefäßfortsätze von ependymalen und subependymalen Zellen bilden in der subependymalen Zone und in der Zona interna des Ratteninfundibulum mit Nervenfasern kompakte neuro-gliöse Faserbündel, die sich in der Zona externa aufzweigen. 2. In allen Zonen des Infundibulum kommen zwischen den Nervenfasern und den Gefäßfortsätzen zahlreiche neuro-gliöse Synapsen vor. In den Gefäßfortsätzen fällt die hohe Zahl an Mikrotubuli sowie die zahlreichen, vielgestaltigen Einschlüsse auf. 3. In der subependymalen Zone sind die Interzellularspalten an bestimmten Stellen außerordentlich weit. Sie haben eine kanalartige Beschaffenheit und enthalten feine Bündel von Nervenfasern. 4. Von den lateralen Anteilen des Infundibulum her erreichen Dendriten von Ganglienzellen des Nucleus infundibularis die Mitte des Infundibulum. In dieser Region sind axodendritische Synapsen anzutreffen. 5. Morphometrische Analysen der Nervenfaserendigungen der Zona externa von Normaltieren zeigen, daß die prozentuale Verteilung der nach Granulagröße differenzierten Nervenfaserklassen für Mitte und Seite der Zona externa etwa gleich ist. Zwischen der Größe der Elementargranula und der Anschnittfläche der zugehörigen Nervenfasern besteht eine direkte Beziehung. 6. Die Nervenfaserendigungen erreichen die Basalmembran des perikapillären Raumes fast ausschließlich im Bereich von gefäßwärts gerichteten Vorwölbungen der Zona externa. Das Ausmaß, in dem Nervenfasern im Vergleich zu den Gefäßfortsätzen von Ependymund Gliazellen den perivaskulären Raum erreichen, ist medial weitaus größer als lateral. 7. Bei bilateral adrenalektomierten Ratten nimmt in bestimmten, vorwiegend lateral gelegenen Nervenfasern die Zahl und Größe der Elementargranula in Abhängigkeit von der Überlebensdauer zu. Dies dürfte auf eine verstärkte Synthese und Speicherung von Corticotropin-Releasing Factor in diesen Nervenfasern zurückzuführen sein. Gegenüber dem Normalbefund ist die neurohämale Kontaktfläche erheblich vergrößert. Der perivaskuläre Raum enthält zerfallene Nervenfaserteile, die durch Bindegewebszellen phagocytiert werden. Diese Veränderungen dürften durch eine unter Versuchsbedingungen verstärkte Wachstumstendenz der Nervenfasern in Richtung auf die Blutgefäße und durch eine Abschnürung der Nerven-faserendigungen ausgelöst werden.
    Notes: Summary 1. In the subependymal and internal zones of the rat median eminence nerve fibres and vascular processes of ependymal and subependymal cells form neuro-glial bundles. They branch in the external zone. 2. In all these three zones of the infundibulum numerous neuro-glial synapses are found between nerve fibres and vascular processes of glial cells. The vascular processes contain a high number of microtubules as well as polymorphous granular inclusions. 3. In certain regions of the subependymal layer the intercellular spaces are enlarged. They form channel-like spaces containing small bundles of delicate nerve fibres. 4. Nerve cells of the infundibular nucleus located in the lateral parts of the infundibulum send dendrites to the medial parts of the infundibulum. In this area axo-dendritic synapses are found. 5. For morphometric analysis, the nerve fibres of the external zone were classified according to the diameter of their granules. It is shown that in the different regions of the external zone the distribution of the various types of nerve fibre is similar. Moreover it can be seen that a direct correlation exists between the size of the sectional plane of a given nerve fibre and the size of the granules it comprises. 6. Nerve fibre endings abutting on the basement membrane of the pericapillary space are mostly found in bulb-like protrusions of the external zone. The extent to which nerve fibres reach the perivascular space—as compared with the vascular processes of ependymal and glial cells—is higher in the medial than in the lateral parts of the infundibulum. 7. In bilaterally adrenalectomized rats the number and diameter of elementary granules increases in nerve fibres located laterally. This increase is directly related to the survival time and may be due to an enhanced synthesis and storage of Corticotropin-Releasing Factor in these nerve fibres. Compared with the findings in untreated animals the neurohemal contact area is significantly enlarged. The perivascular space contains degenerating nerve fibres which are undergoing phagocytosis by connective tissue cells. It is assumed that these alterations are due to the increased growth of nerve fibres towards the vessels of the “Mantelplexus”, and that, following adrenalectomy, this excessive growth leads to a pinching off of nerve fibres.
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    Synaptic structure in the visceral ganglion of the lamellibranch mollusc, Spisula solida (1973)
    Springer
    Cell & tissue research 138 (1973), S. 75-83 
    Details
    ISSN: 1432-0878
    Keywords: Invertebrate ganglia ; Spisula solida ; ynapses ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary An examination using the electron microscope was carried out on the visceral ganglion of the marine bivalve mollusc Spisula solida. A range of fixation, block staining and section staining technique was used to study the structure of chemical synapses. Phosphotungstic acid employed as a block stain specifically stained pre- and post-synaptic structures associated with the membrane at synapses as well as one class of granular vesicle. The specialised contacts were however shown to be rare and in many parts completely absent. Many axons, containing several types of vesicle, were shown to be varicose and it is proposed that they may function in a similar way to the unspecialised varicose terminals of vertebrate autonomic neurons. The role of membrane specialisations in intercellular adhesion is discussed. This study concludes that many synapses may be morphologically unidentified using present criteria.
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    URL: http://dx.doi.org/10.1007/BF00307079
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  • 57
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    The ultrastructure of dentinogenesis and amelogenesis in rat molar tooth germs grown as organ cultures in vitro (1973)
    Springer
    Cell & tissue research 138 (1973), S. 171-186 
    Details
    ISSN: 1432-0878
    Keywords: Tooth germs ; Organ culture ; Differentiation ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Tooth germs from foetal rats of 17 days post-insemination were maintained in vitro for 12 days. Odontoblasts and ameloblasts differentiated and secreted their respective matrices in which mineralization occurred. The ultrastructure of the cells was qualitatively similar to that observed in normal development. Odontoblasts contained more lysosome-like bodies and were found to degenerate in some sites. Mantle dentine was formed but few von Korff fibres were observed. Calcospherites were rarely seen and the mineralizing front of dentine was predominantly linear, associated with numerous small early foci of mineral formation. Enamel showed prism formation associated with the Tomes' process of the ameloblast but some local disturbances in the pattern of enamel formation were observed.
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    URL: http://dx.doi.org/10.1007/BF00306606
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  • 58
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    Electronic Resource
    Effect of histone on the nucleolar morphology of cells cultured in vitro (1973)
    Springer
    Cell & tissue research 138 (1973), S. 273-282 
    Details
    ISSN: 1432-0878
    Keywords: Nucleoli ; Type ; Influence of histone ; Tissue culture ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The frequency of various types of nucleoli was investigated in tissue cultures of human embryonal lung and HeLa cells cultured in the presence of calf thymus histone. The nucleolar morphology and the frequency of various nucleolar types were dependent on the concentration of histone in the tissue cultures of the human embryonal lung cells. HeLa cells required longer cultivation with histone to manifest some effect on nucleoli. In both cases, the observed nucleolar changes suggest the depression of nucleolar RNA synthesis.
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    URL: http://dx.doi.org/10.1007/BF00306612
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  • 59
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    Electronic Resource
    The adrenergic innervation of the renal artery and vein of the rat (1973)
    Springer
    Cell & tissue research 138 (1973), S. 261-272 
    Details
    ISSN: 1432-0878
    Keywords: Extrarenal blood vessels ; Rat ; Adrenergic nerves ; Fluorescence microscopy ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The adrenergic innervation of the extrarenal blood vessels of the rat left kidney was investigated by fluorescence histochemistry and by electron microscopy. The trunk of the renal artery proximal to the aorta is elastic and appears to be very sparsely innervated. In contrast, near the kidney the renal artery—which divides into 3 to 4 large branches of the muscular type possesses a dense adrenergic innervation. The adrenergic terminal axons are situated in the adventitia close to the external elastic lamella, but only rarely in close contact with smooth muscle cells. In most instances several terminal axons are grouped and enclosed by a Schwann cell, single axons being rare. All terminal axons are able to take up and to store 5-hydroxydopamine which strongly suggests that they are adrenergic. The innervation of the renal vein is more sparse than that of the muscular arteries but somewhat denser than that of the elastic artery. In addition, close to the origin of the renal artery the presence of “small intensively fluorescent” (SIF) cells as well as of some adrenergic ganglion cells is noted. The latter are situated in the adrenergic nonterminal axon bundles, which run parallel to the blood vessels. It is concluded that the uneven adrenergic innervation along the artery as well as individual variations in the branching of the artery are the main causes of the unusually high individual variations of the NA content of this organ such as used in pharmacological experiments.
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    URL: http://dx.doi.org/10.1007/BF00306611
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  • 60
    Electronic Resource
    Electronic Resource
    Der praecorneale Glykosaminoglykan-Film beim Haushuhn (1973)
    Springer
    Cell & tissue research 138 (1973), S. 295-298 
    Details
    ISSN: 1432-0878
    Keywords: Cornea epithelium ; Precorneal film ; Domestic fowl ; Ruthenium red ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Das vordere Corneaepithel des Haushuhnes besteht aus 5 Schichten. Die oberflächlichste Lage hat einen Zottenbesatz. Die 0,2 μ langen Mikrovilli haben eine Dicke von 0,04–0,05 μ und einen Abstand von höchstens 0,1 μ voneinander. Auf der freien Oberfläche liegt ein mit Rutheniumrot anfärbbarer Film von Glykosaminoglykanen von 0,26–0,3 μ Dicke. Die Mikrovilli ragen in diesen Film hinein. Dieser Film ist für den spiegelnden Glanz der Cornea verantwortlich. Die Tränensekretion kann sehr gering sein, weil ein Teil der Flüssigkeit an die sauren Mukopolysaccharide gebunden ist. Die Moleküle dieses Films werden wahrscheinlich in der oberen Zellage synthetisiert.
    Notes: Summary The anterior cornea epithelium of the house-hen consists of 5 layers. The outermost layer has a villus border. The 0.2 μ long microvilli are 0.04–0.05 μ thick and are not more than 0.1 μ apart. A film of ruthenium red stainable glycosamine Glycans of 0.26–0.3 μ thickness lies on the free surface. The microvilli protrude into this film. This film is responsible for the reflecting shine of the cornea. The tear secretion can be kept at a low level as a part of the fluid is bound to the acid mucopolysaccharides. The molecules for this film are probably synthesized in the outermost cell layer.
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    URL: http://dx.doi.org/10.1007/BF00306614
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    On the axonal migration of catecholamines in constricted sciatic nerve of the rat (1973)
    Springer
    Cell & tissue research 138 (1973), S. 345-370 
    Details
    ISSN: 1432-0878
    Keywords: Axonal migration ; Catecholamines ; Sciatic nerve ; Radioautography ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The radioautographic technique has been used to study the axonal migration of catecholamines in sympathetic fibres of the sciatic nerve of rats after ligature. A first series of experiments aimed at ascertaining the capacity of the proximal portion of adrenergic fibres to take up and store exogenous tritiated catecholamines (3H-DOPA; 3H-DA and 3H-NA) 3 to 22 hours after ligation. The results are qualitatively similar in rats pretreated and non-pretreated with IMAO, but the intensity of the radioautographic reaction is lower in the latter cases. Most of the labeled axons are filled mainly with vesicular and tubular profiles of endoplasmic reticular origin, large dense bodies (probably lysosomes) and/or mitochondria. The silver grains are generally superimposed on the vesicular and/or the tubular profiles, but in some cases on the large dense bodies, suggesting that exogenous catecholamines can be stored in lysosomes. The question whether SGV specifically store catecholamines also in the modified adrenergic fibres has been investigated in KMnO4 fixed material. These results show that beside a large number of fibres in which there is a strict correlation between labeling and SGV, some fibres containing SGV do not retain the 3H-NA. Conversely some fibres which contain mainly agranular vesicles display radioautographic reaction. Therefore, in case of ligated fibres, SGV cannot be considered the specific organelles for storage of catecholamines. The axonal migration of labeled catecholamines has been studied in animals pretreated with IMAO. A moderate, but selective, labeling is present in the proximal portion of sciatic fibres of rats in which administration of labeled catecholamine preceeded of 2 hours the ligature and this was performed 22 hours before fixation. From these combined types of experiments, it is concluded that despite the presence of all the structures necessary for the storage of a high amount of catecholamines in the modified adrenergic fibres, only a small fraction of catecholamines accumulated above the ligature has been transported by axonal migration. Therefore, the axonal migration of catecholamines appears as an epiphenomenon related to the distal migration of enzymatic and storage proteins from the perikaryon.
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    Long link induction between the microtubules of the manchette in intermediate stages of spermiogenesis (1973)
    Springer
    Cell & tissue research 136 (1973), S. 447-460 
    Details
    ISSN: 1432-0878
    Keywords: Spermatids (rat) ; Manchette ; Microtubules ; Influence of Colcemid ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Links unusual for their length and variable morphology have been described between manchette microtubules in late stages of rat spermiogenesis. In earlier stages of rat spermiogenesis obvious links longer than ∼160 Å are rare, the majority being ∼80 or ∼120 Å in length. The most easily discernible geometric pattern in cross-sections of assemblies of manchette microtubules in intermediate stages of rat spermiogenesis is that of linear arrays sometimes resulting in long and irregularly folded chains of closely linked microtubules. Colcemid disrupts these arrays and is responsible for the formation of more complex geometric patterns. Six hours after drug administration the manchette is dramatically reduced in length. Sheet-like links of variable dimensions and 〉160 Å in length interconnect not only microtubules but C-type microtubules as well as other links. These links are similar in morphology to those found in later stages of rat spermiogenesis. It is suggested that the formation of these links may perhaps be dependent upon aspects of microtubule disassembly.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307363
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    Electron microscopic observations on the mucous secretory activity of the human gallbladder epithelium (1973)
    Springer
    Cell & tissue research 139 (1973), S. 463-471 
    Details
    ISSN: 1432-0878
    Keywords: Gallbladder (Human) ; Mucus secretion ; Reverse pinocytosis ; Histochemistry ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Mucous secretory activity of the human gallbladder epithelium was investigated by light and electron microscopy and with histochemical techniques. There are two types of granules in the supranuclear region of the epithelial cells. The one low in density contains a fine filamentous material and gives a strongly positive silver methenamine reaction. The other is dense and only faintly positive. The granules of the former are considered to be mucous secretory granules and the granules of the latter may be lysosomes. PAS positive granules correspond presumably to both types of granules mentioned above. The mucous secretory granules are considered to be synthesized by the Golgi apparatus and the granular endoplasmic reticulum as has been confirmed in other mucous secretory cells. Their content is released from the cell by reverse pinocytosis. Typical goblet cells occur frequently in the fetal epithelium, but cannot be observed in the adult specimens.
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    URL: http://dx.doi.org/10.1007/BF02028388
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    Riesenmitochondrien im Übergangsepithel der Harnblase des Maulwurfs (1973)
    Springer
    Cell & tissue research 137 (1973), S. 421-434 
    Details
    ISSN: 1432-0878
    Keywords: Giant mitochondria ; Transitional epithelium ; Urinary bladder (mole) ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Das Harnblasenepithel des Maulwurfs wurde elektronenmikroskopisch untersucht. Bei bestimmten Tieren finden sich in den Intermediärzellen „matrixreiche“ Riesenmitochondrien. Diese Riesenmitochondrien scheinen aus normalen Mitochondrien hervorzugehen und enthalten verschieden geformte Einschlüsse. Andere Organe und Zellen bei Maulwürfen mit Riesenmitochondrien in den Intermediärzellen des Harnblasenepithels weisen normale Mitochondrien auf. Es handelt sich demnach hier um eine sog. „Mitochondriose der Intermediärzellen“. Bedeutung, Bildung und Abbau der Riesenmitochondrien werden diskutiert.
    Notes: Summary Giant mitochondria containing an enormous matrix volume were found in the intermediary cells of the urinary bladder transitional epithelium of the mole. Regularly shaped mitochondria develop club-like protrusions forming giant mitochondria that contain several types of inclusions. Various other tissues were examined, but we could state that giant mitochondria exist only in the intermediary cells of the urinary bladder epithelium. Thus a peculiar “intermediary cell mitochondriosis” is described in this paper, not regularly occurring in the mole. The functional significance of the giant mitochondria is discussed.
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    URL: http://dx.doi.org/10.1007/BF00307213
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    Mode de formation des lamelles annelées résultant de l'évolution, en condition anhormonale, des ovocytes de Nereis diversicolor (Annélide Polychète) (1973)
    Springer
    Cell & tissue research 137 (1973), S. 481-492 
    Details
    ISSN: 1432-0878
    Keywords: Annulate lamellae ; Annelidae ; Oogenesis ; Endocrinology ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Résumé L'évolution en l'absence d'hormone cérébrale provoque, dans les ovocytes de N. diversicolor, la formation de lamelles annelées cytoplasmiques (L.A.). Le processus débute par l'apparition de lames de réticulum à proximité du noyau (15 jours d'évolution à 15° C). Les lames se rapprochent ensuite du noyau et des pores apparaissent, situés le plus souvent face à ceux de l'enveloppe nucléaire (20ème jour). La différenciation des lames du réticulum en L.A. progresse ensuite depuis les éléments les plus proches du noyau vers les plus externes. Dans le cytoplasme des ovocytes ayant évolué à la température de 20° C, on assiste en outre à la formation d'empilements de citernes ergastoplasmiques. Ces citernes, non situées à proximité du noyau, subissent également une transformation progressive en L.A. L'étude cytochimique effectuée par digestion enzymatique a montré que la pepsine dégrade fortement le matériel dense des lamelles annelées. Ce matériel semble donc renfermer une teneur élevée en protéines auxquelles pourrait se trouver associé du RNA.
    Notes: Summary Experimental elimination of brain activity leads to the formation of cytoplasmic annulate lamellae (L.A.) in oocytes of N. diversicolor. This process begins with the appearance of cisternae of endoplasmic reticulum in the vicinity of the nucleus (15 days of development at 15° C). Then, the cisternae move closer to the nucleus and pores appear, often facing the pores of the nuclear envelope (20th day). The differentiation of endoplasmic reticulum into L.A. progresses from the elements closest to the nucleus to the more peripheral ones. In the cytoplasm of oocytes which developed at 20° C, stacks of cisternae of rough endoplasmic reticulum have been observed. These cisternae, which are not localized near the nucleus, also undergo a progressive transformation into annulate lamellae. A cytochemical study, based on enzymatic digestion, shows that pepsin strongly digests the dense material of L.A. This material, therefore, seems to contain a high amount of proteins, perhaps associated with RNA.
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    URL: http://dx.doi.org/10.1007/BF00307225
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    Elektronenmikroskopische Untersuchungen an der Froschlunge (1973)
    Springer
    Cell & tissue research 137 (1973), S. 553-561 
    Details
    ISSN: 1432-0878
    Keywords: Lung (frog) ; Alveolar epithelium ; Cytosomes ; Alveolar lining layer surfactant ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Das Alveolarepithel der Froschlunge weist nur einen einzigen Zelltyp auf. Die Zellkörper sitzen in den Nischen zwischen den Kapillaren, die sie mit Zytoplasmaausläufern überdecken. Die Epithelzellen enthalten große Zytosomen mit osmiophilen Lamellen mit einer Periode von 40–42 Å. Sie sind den Typ II-Pneumozyten der Säugerlunge vergleichbar. Das Alveolarepithel der Froschlunge ist mit einer Grenzschicht bedeckt, die in Abhängigkeit von der Fixierung eine 40–42 Å-Periode aufweist oder aus einer oder mehreren Doppelmembranen zusammengesetzt ist. Gittermuster und Myelinfiguren sind vorhanden. Das bedeutet, daß Surfactant in der Froschlunge in gleicher Weise wie in der Säugerlunge dargestellt werden kann.
    Notes: Summary The alveolar epithelium of the frog exhibits only one type of cells. The cell-bodies are situated in the spaces among the capillaries, which they cover with cytoplasmic extensions. The epithelial cells contain large bodies (cytosomes) with osmiophilic lamellae having a period of 40–42 Å. The alveolar cells are considered to be similar to the type II-pneumocytes of mammalian lungs. The alveolar epithelium of the lung of the frog is covered with a lining layer, which depending on the method of fixation consists of periods of 40–42 Å or of one or more double membranes. Lattice formations and myelin figures are seen. This means that the surfactant in the lung of the frog can be demonstrated in the same way as in mammalian lungs.
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    URL: http://dx.doi.org/10.1007/BF00307230
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    Modifications des cellules gonadotropes de la préhypophyse chez la ratte injectée de testostérone au cours du cycle oestral (1973)
    Springer
    Cell & tissue research 138 (1973), S. 63-73 
    Details
    ISSN: 1432-0878
    Keywords: Adenohypophysis ; Rat ; Gonadotrophic cells ; Cycle, influence of testosterone ; Light microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary 4-day cyclic adult female Wistar rats were injected subcutaneously with testosterone propionate on diestrus 1 at 16:00 and on diestrus 2 at 10:00 respectively. Non-injected females served as controls. Autopsy was performed on diestrus 2 at 23:00, and on proestrus at 14:00 and 17:00 respectively. The blue Alcian-PAS staining was used to evidence FSH (β) and LH (γ) pituitary cells. In control animals and in diestrus 2 injected females only a small number of FSH cells could be detected on diestrus 2 at 23:00. This number increased markedly on proestrus at 14:00 and decreased on proestrus at 17:00. A similar evolution was observed in diestrus 1 testosterone injected females, but the number of FSH cells appeared higher at any stage of autopsy in these females than in diestrus 2 injected females and in control rats. In control females, numerous LH cells were observed on diestrus 2 at 23:00. The number of these cells was diminished on proestrus at 14:00 and still more at 17:00. On the contrary few LH cells were detected in testosterone injected females on the evening of diestrus 2. An increase of these cells occurred on proestrus at 14:00, followed at 17:00 by only a weak diminution as established by comparison with control animals. An inhibition of FSH release and a suppression of the proestrus surge of LH were therefore supposed to cause, on one hand, the slowing up of follicular growth observed in diestrus 1 injected females and, on the other hand, the blockage of ovulation noted in both diestrus 1 and diestrus 2 treated animals.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307078
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    An analysis of some aspects of synaptosomal ultrastructure by the use of serial sections (1973)
    Springer
    Cell & tissue research 140 (1973), S. 481-496 
    Details
    ISSN: 1432-0878
    Keywords: Synaptosomes ; Dense projections ; Complex vesicles ; Serial sections ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Synaptosomes and synaptic junctions have been examined employing serial sections, with emphasis placed on four areas of investigation. 1. Starting from unequivocal synaptosomal profiles and tracing them through consecutive sections to the periphery of the synaptosomes, it is clear that vesicles are the one constant feature of the presynaptic terminal. In no instance was it possible to identify an empty membranous profile as synaptosomal. 2. Following a similar procedure it was found that the criteria required to predict the existence of a junctional region within a synaptosomal profile are: the accumulation of synaptic vesicles at one locus within its presynaptic component, and the presence of a postsynaptic profile characterized by two or more junctional features. 3. Serial sections of non-osmicated, PTA stained synaptic junctions confirm the regularity and orderliness of dense projection distribution along the length of the junction. 4. Complex vesicles can usually be followed in two and sometimes three adjacent sections, appearing either as intact vesicles or empty shells. Further observations confirmed that the latter profiles may be sections through the periphery of intact vesicles or through isolated shell fragments. They are more common in the latter form in unbuffered material.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00306675
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    Morphologie und Cytochemie der Harderschen Drüse von Anatiden (1973)
    Springer
    Cell & tissue research 141 (1973), S. 255-283 
    Details
    ISSN: 1432-0878
    Keywords: Glandula harderiana ; Anatidae ; Electron microscopy ; Cytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Die Hardersche Drüse von Anser anser, Anas platyrhynchos und Cygnus olor wurde cytochemisch, licht- und elektronenmikroskopisch untersucht. Vergleichend-anatomisch ergibt sich für die drei Species derselbe Drüsenaufbau. Sie bestehen aus Läppchen bzw. kurzen Tubuli, die von einem einschichtigen, hochprismatischen Epithel ausgekleidet sind, das sich auf die in die Tubuli hineinragenden Leisten bzw. Zotten fortsetzt. Es müssen zentrale und periphere Drüsenabschnitte unterschieden werden. Nur die zentralen Zellen enthalten neutrale und saure Mukosubstanzen. In Semidünnschnitten (Richardson) erweisen sich die peripheren Drüsenzellen als stark vakuolisiert. Zentralwärts nimmt die Vakuolisierung ab, die basale Basophilie nimmt zu. In den Harderschen Drüsen von Gänsen und Enten wurden neben Hydrolasen eine Reihe von Oxydo-Reduktasen aus der Glykolysekette, dem Citratzyklus und der Atmungskette sowie die Enzyme G-6-PDH, GDH, β-HBDH und GLuDH nachgewiesen. Die zentralen Drüsenabschnitte sind mit hohen Aktivitäten der glykolytischen Oxydo-Reduktasen und der NADPH2-liefernden Enzyme ausgestattet. Hydrolytische lysosomale Enzyme, Esterasen und Phosphatasen sind in der Harderschen Drüse nur in geringer Menge vorhanden. Stets können cholinerge Nerven nachgewiesen werden. — Elektronenmikroskopie: Alle Drüsenzellen werden an den apikalen seitlichen Kontaktflächen durch ein differenziertes Schlußleistennetz (Zonulae occludentes, Zonulae adhaerentes, Desmosomen) verbunden. An den zentralen Drüsenzellen lassen sich eine Basalzone (Zellkern, ER), eine ausgedehnte Golgi-Zone und ein apikales Zell-areal unterscheiden. Die Sekretvakuolen entstehen durch Membranabknospung aus dem Golgi-Apparat, ihr Sekret wird durch Ruptur des apikalen Plasmalemms extrudiert. Die peripheren Drüsenzellen weisen strukturelle Merkmale von Becherzellen auf. Ein schmaler basaler Cytoplasmabezirk enthält den komprimierten Kern, der übrige Zellraum überwiegend leere Vakuolen, Membranreste, Lamellenkörper und Lysosomen. Terminale Nervenfasern mit bekannter Innenstruktur durchbrechen die Basalmembran und lagern sich den Drüsenzellen eng an, teilweise in Vertiefungen ihrer basalen und basolateralen Oberfläche eingebettet. Diese terminalen Axone enthalten massenhaft synaptische Vesikel, vereinzelt Bläschen mit einem massendichten Granulum und Mitochondrien. Spezialisierte prae- und postsynaptische Membranen kommen nicht vor.
    Notes: Summary The Harderian gland of Anser anser, Anas platyrhynchos and Cygnus olor has been investigated cytochemically, light microscopically and ultrastructurally. Comparison of these glands within the three species reveals identical structures. The surface of the short glandular tubules carries a single-layered, cylindrical epithelium, which continuously covers all the villi protruding into the tubular lumen. There is evidence for a significant difference between the central and peripheral parts of the glandular tubules. Cytochemical reactions for neutral and acid mucosubstances are positive only in central glandular parts. The semi-thin sections (Richardson staining) show highly vacuolated glandular cells within the peripheral areas. This vacuolisation decreases the more centrally the cells are situated, but conversely their basal basophilic staining increases towards the center. Within the Harderian gland of geese and ducks we have localized by histochemical means several hydrolases and oxydoreductases which reflect enzyme activities of the glycolytic pathway, the citrate cycle, and related metabolic pathways. The activities of glycolytic oxydoreductases and the pentose phosphate pathway enzymes are remarkably high within the central glandular parts. Lysosomal hydrolases, esterases and phosphatases are only to be demonstrated in very moderate degree of activity. All specimens of the Harderian gland contain cholinergic nerves. The ultrastructural analysis reveals highly differentiated apical cellular contacts (zonulae adhaerentes, desmosomes). There are only small intercellular spaces. The centrally located glandular cells can be divided into three compartments, a basal zone containing the nucleus and endoplasmic reticulum, a large Golgi zone and an apical zone containing abundant secretory vacuoles, which emerge from the Golgi apparatus. The secretory products are extruded by rupture of the apical cellular membrane. — The peripheral glandular cells show many similarities to goblet cells. There is only a small basal cytoplasmic are which contains a compressed nucleus, whereas the whole cell is filled with mostly empty vacuoles, membranes, lamellar bodies and lysosomes. — Terminal nerve fibres penetrate the basal lamina and make contact with the glandular cells. The terminal axons contain abundantly synaptic vesicles, sporadic dense cored vesicles and mitochondria. Until now, we could not find any specialized presynaptic or postsynaptic membranes.
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    URL: http://dx.doi.org/10.1007/BF00311357
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    Electron microscopic identification of several types of endocrine cells in the bronchial epithelium of human foetuses (1973)
    Springer
    Cell & tissue research 141 (1973), S. 401-412 
    Details
    ISSN: 1432-0878
    Keywords: Pulmonary epithelium ; Human fetus ; Endocrine cells ; APUD cells ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Electron microscopic investigations of the pulmonary epithelium of human foetuses reveal the occurrence of cells exhibiting fine-structural characteristics of polypeptide hormone producing APUD cells. Three types of cells were identified mainly on basis of the morphology of their secretory granules. Cells of type 1 have the appearance of monoamine storing cells and the dense core of vesiculated granules of these cells are reactive to argentaffine reaction performed directly on ultra-thin sections. Cells of type 2 contain granules of uniform shape and size and of rather homogeneous appearance. Besides in larger bronchial tubules these cells are localized in the epithelium of developing alveoli. Cells of type 3 with large osmiophilic granules tightly bound by a membrane are few and scattered. These cells are observed in larger bronchial tubuli only.
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    URL: http://dx.doi.org/10.1007/BF00307413
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    On structural patterns of the lamina propria of human seminiferous tubules (1973)
    Springer
    Cell & tissue research 141 (1973), S. 413-425 
    Details
    ISSN: 1432-0878
    Keywords: Seminiferous tubules, human ; Lamina propria ; Contractile cells ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The ultrastructure of the lamina propria of human seminiferous tubules was analyzed in normal specimens and compared to biopsies showing great thickenning of this area in light microscopy. The contractile cells are stellate in shape, the intercellular gaps between their branchings being less than 150 Å. The cytoplasmic features of these cells are similar to those described by Ross and Long (1966) and do not differ significantly in the pathological cases examined. The intercellular components, namely collagen fibers, microfibrils and an incomplete basement membrane-like coating of the contractile cells, are strikingly increased in the thickenned lamina propria, although the number of layers making up this structure needs not be increased. Occasionally, the intercellular space is occupied by only one of these materials. The distribution of collagen permits identification of two main patterns in the thickenned lamina propria: a) one where the basement membrane of the seminiferous epithelium is separated from the first layer of contractile cells by a wide collagen zone, and b) another case where the layer displaying greater thickness because of increased collagen deposition is located further away from the germinal epithelium. The functional activity of the contractile cells, the physiological implication of structural alterations of the lamina propria and the necessity to correlate these observations to andrological findings, are discussed.
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    URL: http://dx.doi.org/10.1007/BF00307414
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    Intercellular contact in the unincubated chick embryo (1973)
    Springer
    Cell & tissue research 141 (1973), S. 459-468 
    Details
    ISSN: 1432-0878
    Keywords: Cell adhesion ; Morphogenesis ; Chick Embryo ; Cell membrane ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The unincubated chick blastoderm, which consists of a complete upper epithelial layer of one cell thickness (epiblast) and an incomplete lower layer (hypoblast), was examined with the electron microscope in order to define the types of cell contact present. The terminal contacts between the cells of the epiblast invariably involved several focal tight junctions, but only occasionally involved tight junctions. Desmosomes were not observed in these areas, but were encountered in various phases of development in the deeper contact regions between epiblast cells. This deeper region also showed sporadic focal tight junctions and frequent micropapillae. These micropapillae were also common on the surfaces of hypoblast cells. Intercellular spaces between epiblast and hypoblast cells and within the hypoblast were often wide, narrowing to occasional focal tight junctions. Tight junctions and desmosomes were not observed in association with hypoblast cells. Gap junctions were not observed in any region of the embryo. These observations are discussed in relation to the morphogenetic movements occurring in the forming hypoblast and also the influence of this layer on the subsequent development of the embryo. Comparisons are drawn between the contact morphology in the unincubated blastoderm and that in later stages of development.
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    URL: http://dx.doi.org/10.1007/BF00307117
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    Structural and functional changes in the malpighian tubules of Carausius morosus during dehydration and starvation (1973)
    Springer
    Cell & tissue research 141 (1973), S. 479-492 
    Details
    ISSN: 1432-0878
    Keywords: Malpighian tubule ; Carausius morosus ; Water balance ; Diuretic hormone ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Carausius morosus starved and deprived of water lose about 30% of their body weight in 4 days, mainly due to water loss. Isolated inferior tubules from starved dehydrated insects secrete urine at 0.041 nl·mm−1·min−1 compared with 0.118 nl·mm−1·min−1 in those from fed hydrated insects. This difference is due partly to the level of a diuretic (and perhaps also an antidiuretic) hormone in the haemolymph acting directly on the urine-secreting mechanism and partly to changes in the intrinsic capacity of the tubule cells for urine secretion. This latter change is accompanied by structural changes in the tubules. During starvation and dehydration the lumen becomes packed with white granules, the height of the type 1 cells is reduced, their basal infoldings and brush border become shorter and their mitochondrial volume is reduced.
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    URL: http://dx.doi.org/10.1007/BF00307119
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    Effects of parathyroid hormone on osteoclasts in vivo (1973)
    Springer
    Cell & tissue research 141 (1973), S. 529-544 
    Details
    ISSN: 1432-0878
    Keywords: Osteoclast ; Parathyroid hormone ; Lysosomes ; Bone resorption ; Electron microscopy ; Histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Young rats were treated with high doses of parathyroid hormone (PTH). Osteoclasts from these animals revealed characteristic alterations in comparison to control cells: a) The cytoplasm contained large vacuoles with phagocytosed cells, some of which resembled osteoblasts or osteocytes. The vacuoles were interpreted as lysosomes because the engulfed cells often appeared partly digested and the vacuoles contained acid phosphatase as demonstrated histochemically, b) lipid droplets were present in the cytoplasm and usually located close to the endoplasmic reticulum and/or in regions with many free ribosomes, c) the Golgi complex was more frequently separated from the nuclei than in control cells, d) small coated cytoplasmic bodies were numerous in the peripheral cytoplasm, e) the membranes of the endoplasmic reticulum were fused in some places, f) cytoplasmic regions with numerous free ribosomes were frequent, g) large ring-shaped granules occurred in some mitochondria. Energy dispersive X-ray analysis of these granules provided evidence that they contained calcium and probably phosphorus, h) in some osteoclasts the mitochondria were enlarged. — The findings are consistent with an increased activity of osteoclasts and in particular a stimulation of the lysosomal system in these cells.
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    URL: http://dx.doi.org/10.1007/BF00307123
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    Ultrastructure of leydig cell paracrystalline inclusions, possibly related to reinke crystals, in the normal human testis (1973)
    Springer
    Cell & tissue research 142 (1973), S. 13-26 
    Details
    ISSN: 1432-0878
    Keywords: Paracrystals ; Reinke crystals ; Leydig cells ; Normal human testis ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary One of two types of paracrystalline inclusions, other than Reinke crystals, was encountered in the Leydig cells of the normal mature testes of 9 out of 10 patients. The basic ultrastructure of these formations was that of parallel arrays of fine filaments (8 cases) or tubules (1 case). The filamentous structures, designated as type A paracrystals, consisted of fascicles of parallel rows of 4 or 5 dense fibrils. The latter typically contained dense granules or banded striations spaced at regular intervals. In 4 cases the inclusions were found in the cytoplasm while in 4 others they were confined to the nuclei. The tubular type of paracrystal, type B, was observed in only one patient. Here, groups of closely packed tubular inclusions occupied large portions of cytoplasm. The tubular walls were composed of closely applied, small, circular profiles. In no instance were paracrystals and Reinke crystals encountered in the same cell. A possible role of the former as precursors of the latter is discussed but there is no strong supporting evidence for this despite certain suggestive similarities in unit and subunit measurements.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00306701
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    Post-coital depletion of dense-core vesicles from the external layer of the rabbit median eminence (1973)
    Springer
    Cell & tissue research 142 (1973), S. 27-36 
    Details
    ISSN: 1432-0878
    Keywords: Median eminence (Rabbit) ; Dense-core vesicles depletion ; Releasing factors ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The effects of mating on the depletion of osmiophilic material from dense-core vesicles (DCV) within the external layer of the rabbit median eminence (ME) were investigated. Two different populations of DCV were demonstrated within the ME external layer of the non-mated control animals. A plot of the diameters of these vesicles showed two peaks, one at 90–100 nm, and the other at 120–130 nm. These two populations of DCV were found in separate axons and axon terminals in contact with, or near the perivascular spaces of, portal capillaries. Within these axons and terminals an occasional membrane profile or “vesicle ghost” was also observed. The same two populations of DCV were evident in the ME external layer of animals that were sacrificed at 10 minutes post-coitus. In these experimental animals, the number and size of the smaller population of DCV were the same as those of the none-mated controls. However, there was an obvious decrease in the number of the large (120–130nm) DCV, together with a simultaneous marked increase in the number of “vesicle ghosts”. These ghosts had a mean diameter of 137±14 nm. On the basis of their size, it is suggested that the “vesicle ghosts” represent large DCV that have been depleted of their content following mating. Ultrastructural evidence for the depletion of osmiophilic material from the large DCV of mated animals is provided. According to previous writers the smaller DCV within the ME are aminergic, whereas the larger DCV may contain releasing factors (RF). Our findings provide further morphological evidence to support this hypothesis. Furthermore, RF and biogenic amines appear to be contained within separate nerve terminals in the rabbit ME.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00306702
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  • 77
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    On the regeneration of the eye in Helix aspersa and Cryptomphallus aspersa (1973)
    Springer
    Cell & tissue research 142 (1973), S. 63-68 
    Details
    ISSN: 1432-0878
    Keywords: Snail ; Regeneration of eye ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The distal half of the posterior tentacle of adult Helix aspersa and Cryptomphallus aspersa was removed and the proximal half was studied with light and electron microscopy after different intervals. The tentacle itself does not regenerate, but the receptor organs at the distal end of the normal tentacle differentiate at the level of the section. The newly formed eye is smaller than the control; however, its components and subcellular characteristics resemble those of the normal eye.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00306704
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  • 78
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    Electronic Resource
    Ultrastructural features of rat pituicytes in organotypic culture (1973)
    Springer
    Cell & tissue research 143 (1973), S. 107-116 
    Details
    ISSN: 1432-0878
    Keywords: Neurohypophysis ; Rat ; Pituicytes ; Organotypic culture ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Neural lobes of adult rats have been explanted and organ-cultured for 5–10 days. While perivascular cells and neurosecretory fibers undergo a progressive degeneration, scattered pituicyte-like cells are observed mostly associated with each other in number of two or three cells for group. Cultured pituicytes are quite similar to in vivo pituicytes except for some particular features as the shape of the nucleus and the number of dense bodies. Furthermore they share with the in vivo pituicytes the phagocytic capacity which appears even increased as far as neurosecretory fibers are concerned. Finally, degenerating pituicytes and free lipid droplets are observed in the intercellular spaces: the significance of these facts is discussed and tentatively interpreted. The organotypic culture of the neural lobe seems to represent an experimental model useful to obtain a relatively pure population of pituicytes.
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    URL: http://dx.doi.org/10.1007/BF00307454
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  • 79
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    Ultrastructure de l'épithélium tégumentaire de Glossiphonia complanata (L.) (Hirudinée): cellules épithéliales et organes sensoriels de Bayer (1973)
    Springer
    Cell & tissue research 143 (1973), S. 355-365 
    Details
    ISSN: 1432-0878
    Keywords: Integument ; Hirudinea ; Sensory organs ; Nerves ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Résumé Le tégument de Glossiphonia complanata comprend, outre les cellules épithéliales banales, de nombreux organes particuliers ou organes de Bayer, regroupés essentiellement sur la face dorsale de l'animal. Ils sont formés par une cellule apicale saillante enchassée dans une cellule musculaire en anneau. L'ensemble de ces formations est étudié du point de vue ultrastructural. Des cellules épithéliales partent des fibres nerveuses afférentes, sans doute vecteur des perceptions de stimuli mécaniques de pression au niveau du tégument; la réponse se faisant sans doute par la contraction de la cellule basale de chaque organe de Bayer, innervée par des fibres nerveuses efférentes, entrainant la saillie de la cellule apicale. Le hérissement de ces nombreuses papilles du tégument dorsal pourrait être un signal perçu par le partenaire sexuel, chez cette Hirudinée à fécondation hypodermique.
    Notes: Summary The integument of Glossiphonia complanata, built up by epithelial cells, contains numerous particular organs (Bayer organs), mainly on the dorsal side of the animal. They consist of a protuberant apical cell, which is surrounded by a ring-shaped muscle cell. All the integumentary formations are studied from an ultrastructural point of view. From the epithelial cells issue afferent nerve fibres, considered as vectors of the perception of mechanical stimuli of pressure at the level of the integument; the response no doubt operating by the contraction of the basal muscle cell of each Bayer organ, innervated by efferent nerve fibres, bringing forth the protrusion of the apical cell. The erection of these numerous papillae of the dorsal integument might be a signal perceived by the sexual partner, fecundation occurring in this group of leeches under the integument.
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    URL: http://dx.doi.org/10.1007/BF00307421
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  • 80
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    New types of endoplasmic reticulum in neurons of the paracervical ganglion of the rat (1973)
    Springer
    Cell & tissue research 143 (1973), S. 505-515 
    Details
    ISSN: 1432-0878
    Keywords: Endoplasmic reticulum ; Paracervical ganglion (Rat) ; Neurosecretion ; Electron microscopy ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Two new types of structure of the cytoplasm of an autonomic neuron were described: (1) Inclusions of the rough ergastoplasmic reticulum, which were named “ergastosomes”, contain finely granular material with moderately electron opaque staining with both glutaraldehyde-OsO4 and KMnO4 fixations. A short comment was made on the nature of the contents of the ergastosomes. (2) Tubular formations of endoplasmic reticulum were found in the axons and/or dendrites of the paracervical ganglion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00306769
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  • 81
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    A comparison of the fine structure of thoracic and abdominal interganglionic connectives in the newly hatched and adult stick insect, Carausius morosus Br. (1973)
    Springer
    Cell & tissue research 145 (1973), S. 299-309 
    Details
    ISSN: 1432-0878
    Keywords: Nervous system ; Stick insect ; Blood-brain barrier ; Development ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The fat body sheath which surrounds the ventral nerve cord of the adult stick insect, Carausius morosus, is absent in the hatchling. Since the haemolymph sodium ion compositions of the two stages are similar, it is suggested that the fat body sheath is not a site of a blood-brain barrier dealing with extra-axonal regulation of sodium. Similarities of ultrastructure in connectives of adults and hatchlings are demonstrated, and tight junctions are shown to occur in perineural cells of both stages. The possible significance of this to the blood-brain barrier of this insect is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307160
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  • 82
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    On the impulse conducting system of the monkey heart (Macaca mulatta) (1973)
    Springer
    Cell & tissue research 145 (1973), S. 363-388 
    Details
    ISSN: 1432-0878
    Keywords: Heart ; Monkey ; A-V node and bundle ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The atrio-ventricular (A-V) node of the monkey heart is located in the focus of converging atrial muscle. Three main atrial muscle strands, coming from the atrio-ventricular ring, the dorsal wall of the atria, and the ventral part of the atrial septum, converge in the nodal region where they overlap and are interconnected. The junctional type of fibers establishing interconnection between the atrial muscle and the nodal tissue are not strictly localized at the periphery of the node, but may be traced further, along the A-V ring and coronary sinus. The A-V node consists of a loose peripheral and a compact distal part. In the former, typical nodal fibers were found, while the compact part shows an important individual variation in structure and cell-types. In some monkey hearts, the nodal fibers gradually become broader bundle fibers, while in other specimens the junctional fibers surround the compact part and than penetrate the nodal-His (N-H) region. These junctional fibers become nodal fibers or are in terminal contact with large clear cells up to 50 μ in diameter. Clear cells of various diameters are often intercalated between the cell rows of the nodal and His-bundle fibers and may form a distinct cellular gate between the node and the His-bundle.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307163
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  • 83
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    Sarcomere formation in chick striated muscle (1973)
    Springer
    Cell & tissue research 145 (1973), S. 167-170 
    Details
    ISSN: 1432-0878
    Keywords: Striated muscle ; Chick ; Sarcomere formation ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Sarcomere assemblage in striated muscle of the early developing chick embryo was studied with the electron microscope. In myogenic chick somites, non-striated myofibrils are seen with the electron microscope, prior to striated ones. These crude myofibrils are traversed at regular periodic intervals by a tubular system which is associated with dense Z-line material shortly after its appearance. Longer sarcomeres as well as banding patterns similar to those found in mature striated muscle follow and possibly depend on prior Z-line formation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307385
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  • 84
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    The fine structure of the conducting system of the monkey heart (Macaca mulatta) (1973)
    Springer
    Cell & tissue research 145 (1973), S. 191-211 
    Details
    ISSN: 1432-0878
    Keywords: Heart ; Monkey (Macaca mulatta) ; Conducting system ; Atrium ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In the sino-atrial (S-A) node of the monkey heart two types of muscle cells occur: 1. typical nodal cells which are the predominant cells and form the nodal fibers. 2. “Intercalated clear cells” with various diameters (4 to 12 μm) and containing poorly developed myofibrils, rich in glycogen and demonstrating poor staining properties. These latter cells are dispersed, few in number, and never form discrete fibers of themselves, but are intercalated between the cell rows of the typical nodal fibers. Such intercalated clear cells become more numerous at the periphery of the node. Interconnection between the S-A node and the conventional atrial muscle is established by a progressive transformation of nodal fibers into atrial fibers producing an intermediate (or junctional) type of fiber at the nodal periphery. However, in addition, few nodal fibers make direct contact with the atrial cardiocytes. Our light and EM studies have failed to prove the existence of truly specialized internodal pathways. Nevertheless intercalated clear cells, nodal-like cells, junctional or intermediate type of cells are relatively frequent in valvular regions (Thebesian, Eustachian, A-V, fossa ovalis) and less frequent in other regions of the atrial wall.
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    URL: http://dx.doi.org/10.1007/BF00307388
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  • 85
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    Seasonal changes of the ultrastructure of the pars tuberalis of the hypophysis of Rana temporaria (1973)
    Springer
    Cell & tissue research 145 (1973), S. 459-469 
    Details
    ISSN: 1432-0878
    Keywords: Pars tuberalis ; Rana temporaria ; Seasonal changes of ultrastructure ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In the pars tuberalis of the hypophysis of Rana temporaria, which shows the ultrastructural characteristics of a polypeptide hormone secreting endocrine gland, seasonal changes of the ultrastructure are described. In accordance with the literature, these seasonal changes of ultrastructure are interpreted as the morphological expression of seasonal changes of endocrine activity of the pars tuberalis.
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    URL: http://dx.doi.org/10.1007/BF00306718
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  • 86
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    Electron microscopic study of rat pituitary primordium in organ culture (1973)
    Springer
    Cell & tissue research 146 (1973), S. 453-461 
    Details
    ISSN: 1432-0878
    Keywords: Hypophysis ; Primordium ; Rat ; Organ culture ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary To see whether adenohypophysial tissue has the capacity of self-differentiation, Rathke's pouch together with part of the neurohypophysial primordium was isolated from 12-(Group A), 14- (Group B) and 15-day-old (Group C) rat fetuses and cultivated for 9, 6 and 5 days, respectively. Electron microscopic examination of these explants showed many adenohypophysial cells containing electron dense granules, indicative of cytodifferentiation in all groups. Three cell types were distinguishable on the basis of the size of their granules. Variable amounts of neurohypophysial tissue were seen in some explants of Groups B and C, but not A. This “pars nervosa” contained pituicytes with lipid-like inclusions in their cytoplasm but no neurosecretory axons. The adenohypophysial cells abutting on the pars nervosa were arranged rather regularly like cells of the pars intermedia which contained a few granules of about 200 mμ diameter. From these data it is concluded that in rats granule formation in the adenohypophysial primordial cells starts in the absence of hypothalamic neurosecretory substances. It is uncertain from this study whether these cells actually contain hypophysial hormones.
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    URL: http://dx.doi.org/10.1007/BF02347175
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  • 87
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    Electronic Resource
    Studies on the effect of denervation in developing muscle (1973)
    Springer
    Cell & tissue research 147 (1973), S. 75-85 
    Details
    ISSN: 1432-0878
    Keywords: Skeletal muscle ; Denervation ; Development ; Fiber types, myofibrils ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Ultrastructural diversification of muscle fibers, with regard particularly to myofibrillar changes, has been investigated in the fast-twitch extensor digitorum longus (EDL) and the slow-twitch soleus muscles of the rat during fetal and postnatal development in the presence and in the absence of motor innervation. The band pattern and the shape of the myofibrils were uniform in fetal and neonatal muscle fibers and underwent differential changes during the first weeks after birth, concomitantly with fiber type specialization. The most evident variations in myofibrillar structure arising in this period concern the thickness of the Z band and the arrangement of the myofibrils. Myofibril formation was at first not impaired by denervation of rat muscles performed in utero and, although focal disintegration of myofibrils and detachment and loss of orientation of filaments became apparent by one week, atrophic muscle fibers with well-organized myofibrils could be seen as late as 2 months after birth. However, denervated muscle fibers of EDL and soleus did not display any significant and consistent difference in myofibrillar band pattern and shape. No variation in mitochondrial content and sarcoplasmic reticulum development was likewise seen in muscle fibers of EDL and soleus after fetal denervation. The findings emphasize the importance of neuromuscular interactions in muscle differentiation.
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    URL: http://dx.doi.org/10.1007/BF00306601
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  • 88
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    Effect of cycloheximide on the fine structure of corpus luteum in intact and hypophysectomized rats (1973)
    Springer
    Cell & tissue research 146 (1973), S. 223-235 
    Details
    ISSN: 1432-0878
    Keywords: Corpus luteum (Rat) ; Cycloheximide ; Hypophysectomy ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In rats, one large intravenous dose of cycloheximide leads to extensive development of two types of membrane-formations in the cells of corpora lutea, within two hours. Both the laminated dense bodies (concentric layers of smooth membranes showing high electron density) and the tubular aggregates (tightly packed smooth tubules with diameter smaller than usual) exhibited obvious connections with endoplasmic reticulum membranes. The reorganization of tubular aggregates gave rise to “crystalloids” showing hexagonal symmetry. The crystalloids, being obviously unstable, were transformed into smooth fingerprints (concentric arrays of paired agranular membranes showing the same density as endoplasmic reticulum membranes). Hypophysectomy, performed 24 hours previously, moderated but did not totally abolish the development of membranous configurations. The described effect of cycloheximide is considered to represent cellular injury, probably due to membrane-denaturation.
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    URL: http://dx.doi.org/10.1007/BF00307348
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  • 89
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    Ultrastructure of subcellular fractions of bull spermatozoa after hyamine treatment (1973)
    Springer
    Cell & tissue research 144 (1973), S. 395-408 
    Details
    ISSN: 1432-0878
    Keywords: Spermatozoa ; Cattle ; Influence of cationic surface active agent ; Acrosome reaction ; Centrifugation ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Ejaculated bull spermatozoa (SZ) were washed and incubated with a cationic surface active agent, Hyamine 2389, and centrifuged using 2-step discontinuous sucrose density gradient. The washed SZ, Hyamine-treated SZ and subcellular spermatozoal fractions obtained after centrifugation were prepared for electron microscopy. The washing did not cause any major structural changes in SZ. The Hyamine treatment of SZ disrupted the outer acrosome membranes. The anterior part of acrosome (the acrosomal cap) was detached retaining its integrity, or forming vesicles by fusing with the cell membrane as in “true” acrosome reaction. Because of this structural similarity in vesicle formation, Hyamine is assumed to be a suitable experimental initiator for acrosome reaction. The loosened acrosomal membranes were harvested almost totally by the centrifugation. The acrosomes were seen as loosened U-shaped unbroken acrosomal caps or as vesicles with fuzzy acrosomal material. The lightest particles were vesicles consisting of smooth membranes, formed evidently of sperm cell membrane. A negligible amount of fibrous sheaths were also among acrosomal membranes but no other sperm parts were encountered.
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    URL: http://dx.doi.org/10.1007/BF00307584
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  • 90
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    The ultrastructure of the duct system in the rat extraorbital lacrimal gland (1973)
    Springer
    Cell & tissue research 144 (1973), S. 453-466 
    Details
    ISSN: 1432-0878
    Keywords: Lacrimal gland ; Rat ; Duct system ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The duct system of the rat exorbital lacrimal gland consists of intercalated ducts, interlobular ducts and excretory ducts. The morphological changes from one type of duct to the next are gradual. At the light microscopical level this consists of a change from a bilaminar epithelium in the intercalated ducts to an epithelium, consisting of approximately three layers — which may be pseudostratified — in the excretory ducts. The basal layer of the intercalated ducts consists of myoepithelial cells, whereas the inner epithelial cells may have both a secretory and an electrolyte transporting function. The interlobular duct epithelium contains many cells with deep infoldings of the basolateral plasma membranes and associated mitochondria, suggesting a similar function to the striated duct epithelium in salivary glands. Numerous basal cells in this epithelium have tentatively been interpreted as unusual myoepithelial cells. Nerve terminals have been observed in the ductal epithelium.
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    URL: http://dx.doi.org/10.1007/BF00307373
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  • 91
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    Effects of colchicine on myofilament arrangement and the lysosomal system in skeletal muscle (1973)
    Springer
    Cell & tissue research 144 (1973), S. 467-473 
    Details
    ISSN: 1432-0878
    Keywords: Muscle ; Colchicine ; Myofibrils ; Acid phosphatase ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Colchicine was intraperitoneally administered chronically to adult male Sprague-Dawley rats. The ultrastructural study of hind-limb muscles revealed that myofilament desorientation resulted. Bundles of myofilaments were found coursing perpendicular or oblique to the longitudinal axis of the muscle fiber. It is concluded that a colchicine-sensitive factor is involved in maintaining normal orientation of myofibrils in mature muscle. Also found in the sarcoplasm of the colchicine treated animals were complex spheromembranous bodies. These bodies enveloped mitochondria or other organelles and appeared to be derived from the sarcoplasmic reticulum. The lysosomal nature of these bodies is indicated by the localization of acid phosphatase activity in them. Acid phosphatase activity was also displayed in the sarcoplasmic reticulum. The spheromembranous bodies seem to be part of a sarcotubulo-lysosomal system in skeletal muscle.
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    URL: http://dx.doi.org/10.1007/BF00307374
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  • 92
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    Aspects infrastructuraux de la sécrétion dans les cellules glandulaires du canal déférent chez Porcellio scaber Latreille (Isopode terrestre) (1973)
    Springer
    Cell & tissue research 144 (1973), S. 523-537 
    Details
    ISSN: 1432-0878
    Keywords: Glandular cells ; Isopoda ; Golgi apparatus development ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Résumé Dans ce travail, les auteurs présentent les résultats de leurs recherches, au niveau de la microscopie électronique, concernant les processus sécrétoires dans les cellules glandulaires de la partie antérieure, réniforme, du canal déférent chez Porcellio scaber Latreille. Les observations sont dirigées spécialement sur l'appareil de Golgi qui est poursuivi dans toutes les phases d'élaboration de la sécrétion, dans laquelle il paraît avoir un rôle essentiel. Après avoir obtenu la conviction que, dans le processus délaboration de la sécrétion, les dictyosomes s'usent complètement et disparaissent, les auteurs recherchent l'origine de nouveaux dictyosomes qui participeront au cycle de sécrétion suivant.
    Notes: Summary In this paper the authors present an investigation at infrastructural level concerning the secretory process in the glandular cells of the anterior kidney-like portion of the deferent ducts in Porcellio scaber Latreille. The observations are directed especially to the Golgi apparatus in all phases of elaboration of secretion in which it seems to have an essential role. After ascertaining that in the process of elaboration of secretion the dictyosomes waste away and disappear, the authors investigated the origin of the new dictyosomes that become involved in the following secretory cycle.
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    URL: http://dx.doi.org/10.1007/BF00307378
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  • 93
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    Cytochemical demonstration of catalasepositive particles (peroxisomes?) in fibroblasts (1973)
    Springer
    Cell & tissue research 144 (1973), S. 539-547 
    Details
    ISSN: 1432-0878
    Keywords: Catalase ; Peroxisomes ; Fibroblasts ; Enzyme cytochemistry ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The connective tissue of the mucosa of the respiratory tract, of the gastric mucosa and of the mucosa of the tongue was investigated in mice. The tissue was fixed in glutaraldehyde and incubated in an alkaline DAB-medium to demonstrate the peroxidatic activity of catalase. In fibroblasts and fibrocytes, as well as in lymphoid cells, membrane bounded particles from 0.10 to 0.25 μm in diameter were found, whose matrices were intensely stained by the histochemical reaction. The reaction is inhibited by the addition of 2×10−2 M 3-amino-1,2,4-triazole. In connective tissue cells of specimens, which were not reacted to demonstrate catalase activity, these organelles show a granular matrix of moderate electron density. They lack a crystalline core. The possibility that these catalase-positive particles (CPs) represent peroxisomes is discussed.
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    URL: http://dx.doi.org/10.1007/BF00307379
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  • 94
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    Quantitative data on the fine structural organization of the palisade zone of the median eminence (1973)
    Springer
    Cell & tissue research 136 (1973), S. 111-120 
    Details
    ISSN: 1432-0878
    Keywords: Median eminence ; Rat ; Granular vesicles ; Ultrastructure ; Quantitative evaluation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In order to characterize different sub-zones in the palisade zone of the rat median eminence, quantitative ultrastructural parameters were applied to brains fixed in aldehyde-osmium tetroxide. The palisade zone has been subdivided in 4 successive sub-zones. Increasing numbers of granular vesicles (GV), especially those smaller than 110 nm, are observed from dorsal to ventral sub-zones. There also are more GV per unit area of nervous tissue in the perivascular than in the more dorsal sub-zones. The individual nerve profiles exhibit a larger size in the perivascular layer than in the more dorsal areas, whereas the number and size of nerve profiles devoid of vesicles diminish from dorsal to ventral. As a consequence more GV occur in the perivascular nerve profiles. In the GV containing nerve profiles the concentration of GV is, however, constant in the different sub-zones. A fluctuating size of the preterminal and terminal parts of the nerve fibres is suggested.
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    URL: http://dx.doi.org/10.1007/BF00307683
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  • 95
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    Zur Feinstruktur einer elektrischen Synapse (1973)
    Springer
    Cell & tissue research 136 (1973), S. 139-152 
    Details
    ISSN: 1432-0878
    Keywords: Giant fiber ; Earthworm ; Septum ; Electrical synapse ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Die erneute elektronenmikroskopische Untersuchung der dorsalen Riesenfasern der Lumbriciden Lumbricus terrestris (L.t.) und Eisenia foetida (E.f.) zeigt, daß es sich bei den segmentalen Septen um Strukturen bisher unbekannter Komplexität handelt. Bei beiden Tierarten beträgt der Septalspalt nur 65 Å (E.f.) bzw. 75 Å (L.t.) und ist deshalb als „gap junction“ anzusprechen. Daneben fallen folgende Differenzierungen am Septum auf: „septate junctions” (L.t. und E.f.), „intermediate junctions“ (L.t. und E.f.), beidseitig am Septum gelegene und nicht von einer Membran umschlossene Membranappositionen (E.f.), sowie einseitig am Septum gelegene „dense projections“ (L.t.). Bei L.t. kommen außerdem auf beiden Seiten des Septums Vesikel vor, die von einer Elementarmembran umschlossen sind und nach Größe (φ ca. 575 Å), Lage, Haufenbildung und elektronenoptischem Habitus den Vesikeln chemisch übertragender Synapsen gleichen. Die Befunde werden hinsichtlich ihrer möglichen Bedeutung bei der elektrischen Übertragung und als Indizien für das Vorliegen einer gemischten Synapse diskutiert.
    Notes: Summary Electronmicroscopical examination of the dorsal giant fibers in the earthworms Lumbricus terrestris (L.t.) and Eisenia foetida (E.f.) reveals that their segmental septa are structures of so far unknown complexity. In both species the extracellular cleft between the two axon membranes of the septum amounts to only 65 Å (E.f.) and 75 Å (L.t.) respectively and is therefore regarded as “gap junction”. The following other structural differentiations of the septum were observed: “septate junctions” (E.f. and L.t.), “intermediate junetions” (E.f. and L.t.), densities apposed to both sides of the septum and not surrounded by a membrane (E.f.), and densities resembling “dense projections” on one side of the septum only (L.t.). In addition the septa of L.t. show vesicles on both sides which are bounded by a unit membrane and resemble the vesicles of chemically transmitting synapses in size (φ ca. 575 Å), location, accumulation, and electronoptical habitus. The significance of the findings in regard to electrotonic transmission and the possible existence of a mixed synapse is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307685
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    Ultrastructural changes in rat epididymis after vasectomy (1973)
    Springer
    Cell & tissue research 136 (1973), S. 177-182 
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    ISSN: 1432-0878
    Keywords: Rat ; Epididymis ; Effect of vasectomy ; Spermatozoa ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Epididymal biopsies from rats that had undergone unilateral or bilateral vasectomies from one to eight months previously were compared with biopsies from their contralateral side or from normal controls to ascertain what ultrastructural changes had occurred. After vasectomy, spermatozoa appeared to dissolve in the lumen of the caput epididymidis and to be absorbed by the principal cells. About 5 weeks after vasectomy, numerous lamellar accumulations became apparent in the supernuclear region. Their resemblance to lysosomes or residual bodies was confirmed by an acid phosphatase reaction. After 10 weeks, similar lamellar and polymorphic accumulations on the contralateral side of animals with unilateral vasectomies indicated that resorption had also increased on the unligated side.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307438
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  • 97
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    Direct demonstration of eight-fold symmetry in nuclear pores (1973)
    Springer
    Cell & tissue research 136 (1973), S. 183-190 
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    ISSN: 1432-0878
    Keywords: Nuclear pore ; Amphibian oocyte ; 8-fold symmetry ; Fibrous network ; Electron microscopy ; Negative staining
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Pictures, in front view, are presented of the nuclear pores from the oocytes of the newt Taricha granulosa. Negative staining is used. It is directly visible, on a substantial proportion of the pores, that the number of subunits in the annulus is 8. This conclusion had been reached earlier by other writers, who had used the rotation technique to ascertain the radial symmetry. The rotation technique is known to be very unreliable, though on this occasion had produced the correct result. A fibrous mesh network, connecting the subunits of separate pores is described.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307439
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  • 98
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    Ultrastructure of the chick thymus (1973)
    Springer
    Cell & tissue research 136 (1973), S. 191-205 
    Details
    ISSN: 1432-0878
    Keywords: Thymus ; Fine structure ; Avian ; Gallus domesticus ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The ultrastructure of the normal thymus of the young chicken (Gallus domesticus) is described. Four main cell types, lymphoid cells, epithelial cells, macrophages and myoid cells, can be distinguished. The lymphocytes are more numerous in the cortex than in the medulla, and are quantitatively the most important component of the thymus. The epithelial cells vary greatly in morphology. Reticular epithelial cells, which have long cytoplasmic processes connected by desmosomes, and which appear to afford a supporting network for the free cells of the thymus, are present in the cortex and medulla. Undifferentiated epithelial cells are present in the medulla and cortico-medullary regions and have few intracytoplasmic fibrils or desmosomes. Cystic epithelial cells, showing intercellular and intracellular cyst formation are frequent in the medulla. Also present in the medulla are squamous epithelial cells which contain many intracytoplasmic fibrils and have numerous desmosomes, and which are involved in the formation of Hassall's corpuscles. Macrophages are present in moderate numbers in the cortex and medulla, and immature and fully developed myoid cells are common in the medulla. Other cell types present include granule-containing cells with desmosomes, large pale cells with few cytoplasmic organelles, mast cells, plasma cells, red blood corpuscles and cells of the granular leukocyte series.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307440
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  • 99
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    Spermatogenesis revisited (1973)
    Springer
    Cell & tissue research 138 (1973), S. 155-169 
    Details
    ISSN: 1432-0878
    Keywords: Spermiogenesis ; Mouse ; Multinucleate spermatids ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary This ultrastructural study confirms and extends the light microscope findings of Bryan (1971) concerning the presence and developmental fate of multinucleate spermatids. Four main classes of cells: uninucleate-individual, uninucleate-conjoined, multinucleate-conjoined, and multinucleate-individual, were identified along with a few instances of more complex syncytial organizations. When the respective nuclei in a given multinucleate are far enough apart, each develops autonomously but in synchrony with its neighbors. When nuclei are intimately associated, the “normal” pattern of spermiogenesis may be altered, giving rise to highly bizarre spermatozoa. Commonly, a single Golgi complex serves a pair of nuclei and gives rise to a “T-shaped” acrosome which binds the nuclei together. During the ensuing nuclear elongation phase, such units are invested by a single manchette. Pairs of axonemes within a common plasma membrane have also been encountered. These ultrastructural findings indicate that multinucleate spermatids are true components (not artifacts) of the seminiferous epithelium of normal animals. The presence of such cells and the unusual developmental consequences which can arise as a result of the multinucleate state must be taken into account when evaluating the course of spermatogenesis in cases of mutation- or chemically-induced infertility.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00306605
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  • 100
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    Histochemical demonstration of Δ5-3 ß-OHD activity in the granulosa cells of ovarian follicles of immature and mature mice correlated with some ultrastructural observations (1973)
    Springer
    Cell & tissue research 136 (1973), S. 215-228 
    Details
    ISSN: 1432-0878
    Keywords: Ovary (mouse) ; Granulosa cells ; Steroidogenesis ; Electron microscopy ; Histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A systematic study of Δ5-3β-hydroxysteroid dehydrogenase in the granulosa cells of immature and mature mice was made. The histochemical results were compared with the ultrastructural findings on the same cells in an attempt to determine whether the granulosa cells are capable of a steroidogenic role. In newborn and immature mice the granulosa cells of a great amount of follicles demonstrated a moderate or strong histochemical activity. In mature mice the granulosa cells demonstrated a weak or moderate activity normally only in preovulatory follicles and in some other atretic follicles. The granulosa cells of the normal developing follicles did not show such activity. In addition the histological control of numerous parallel sections demonstrated particularly in immature ovaries the presence of a great amount of atretic follicles. In the cytoplasm of the granulosa cells of the follicles in immature ovaries only clusters of lipid droplets with ribosomes were noted; while in the preovulatory follicles of mature animals there started to appear mitochondria with tubular cristae, smooth membranes of the endoplasmic reticulum and irregular lipid droplets. In the obviously atretic follicles several granulosa cells as well as theca interna cells showed numerous lipid droplets and ribosomes together with different degenerating organelles. The granulosa cells of the normal developing follicles showed a well developed Golgi complex, granular endoplasmic reticulum and free ribosomes. The histochemical and ultrastructural findings suggest a steroidogenic role of the granulosa cells only in the larger preovulatory follicles (probably related to an early luteinization of this layer) but this role was not demonstrated in the same cells in normal developing follicles. In addition, since an histochemical positivity was demonstrated also in the granulosa cells of some obviously atretic follicles, it is possible that many of the follicles having granulosa cells filled with lipid droplets and attached ribosomes and histochemically positive might be, in the immature ovaries, in a very precocious stage of atresia. It is to precise for these cells whether a cytoplasm with these two strictly correlated components (lipid droplets and attached ribosomes) and showing an histochemical positivity could carry-on all the biochemical steps involved in steroid biosynthesis or only has only a temporary capability to produce some precursors of steroids.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307442
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