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  • 1975-1979  (3,654)
  • 1920-1924  (688)
  • Life and Medical Sciences  (3,779)
  • Ultrastructure  (563)
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  • 1
    Electronic Resource
    Electronic Resource
    Ultrastructural changes in the distal wing bud of the chick embryo after removal of the apical ectodermal ridge (1979)
    Springer
    Development genes and evolution 185 (1979), S. 333-346 
    Details
    ISSN: 1432-041X
    Keywords: Chick embryo ; Limb bud ; Ultrastructure ; Cell death
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The ultrastructural changes in the wing bud afterapical ectodermal ridge (A.E.R.) removal was studied to re-examine the issue of distal mesenchymal cell death. The A.E.R. of the right wing bud was removed microsurgically from chick embryos of stages 18 to 22 (HH 1951). The wing buds were examined at three hour intervals up to twelve hours after the operation with light, transmission and scanning electron microscopy. The main findings were: (1) Immediate and temporary shrinkage of the mesenchymal extracellular space 100 to 150 μm and chromatin condensation in the cells 50 to 75 μm from the wound. (2) Death of ectodermal and mesenchymal cells in the immediate vicinity of the wound. (3) Formation of a single squamous-like layer of mesenchymal cells to cover the wound. (4) Occasional evidence of cell death in the distal mesenchyme at later times after the operation. The pattern of cell death observed suggests only a traumatic etiology, and gives little evidence for the postulated developmental significance of cell death following A.E.R. removal.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00848520
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  • 2
    Electronic Resource
    Electronic Resource
    Ultrastructure of collagen in sea urchin embryos (1979)
    Springer
    Development genes and evolution 186 (1979), S. 65-70 
    Details
    ISSN: 1432-041X
    Keywords: Sea urchin ; Embryo ; Collagen ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Collagen fibrils with a main period banding of 610 Å and 220 Å in width were observed in the blastocoel of 72-h embryos of the sea urchin,Strongylocentrotus purpuratus. Non-striated fibrils of 50 Å diameter were also observed. The collagen is seen in highest concentration in the vicinity of mesenchyme cells which are richly endowed with endoplasmic reticulum and secretory vesicles. A role for collagen in cell attachment, orientation and spicule formation is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00848108
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  • 3
    Electronic Resource
    Electronic Resource
    Ultrastructural and biochemical observations on the metanephros of normal and cultured chick embryos (1979)
    Springer
    Anatomy and embryology 155 (1979), S. 95-105 
    Details
    ISSN: 1432-0568
    Keywords: Metanephros ; Chicken embryo ; Ultrastructure ; Parathyroid hormone ; Cyclic AMP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A comparative electron microscopical study was conducted on the metanephros from chick embryos differentiated either in shell-less culture or in ovo. Developmental characteristics were very similar in both cases. Up to stage 37 (Hamburger-Hamilton) the metanephros contained large numbers of immature nephrons; their renal corpuscles were crescent-shaped and consisted of an outer layer of flat cells and an inner one of cuboidal cells. In more advanced corpuscles also found at this stage the inner layer had formed numerous rudimentary pedicels and the tunica media of the glomerular arteriole contained juxta-glomerular cells with numerous, small, electron dense granules. In the metanephros from embryos at stage 38 or older, large numbers of nephrons had completed their differentiation; their rounded renal corpuscles had fully differentiated podocytes with thin interdigitating pedicels and the proximal convoluted tubules had numerous apical microvilli, vesicles, vacuoles and tubular invaginations indicating an active process of resorption. These results appear to indicate that both in culture and in ovo-developed embryos, the metanephri start to function around stage 38. In the case of normal embryos this conclusion agrees with previous physiological and biochemical determinations. The injection of 20 USP parathyroid hormone into 16-day old chick embryos produced an increase in the concentration of cyclic AMP in the metanephros. This favours the idea that the regulation of kidney function by the hormone begins during the embryonic period.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00315734
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  • 4
    Electronic Resource
    Electronic Resource
    Ultrastructural observations on closure of the neural tube in the mouse (1979)
    Springer
    Anatomy and embryology 156 (1979), S. 73-88 
    Details
    ISSN: 1432-0568
    Keywords: Closure ; Development ; Mammalina embryo ; Neural tube ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The fusion of the neural walls in the cephalic part of mouse embryos varying in age from 9 to 20 somites was examined with the electron microscope. In the rhombencephalic region the rim of the neural wall was formed from outside inward by ectodermal surface cells, a row of flattened cells without surface projections and neuroepithelial cells. At the junction of the surface ectoderm and the flat cells were seen large projections containing a cytoplasmic matrix without organelles and previously referred to as “ruffles”. The initial contact between the walls was made by the large cytoplasmic arms and numerous finger-like projections interdigitating with similar projections from the opposite wall. The projections originated from the surface ectoderm and possibly neural crest cells. During further fusion the surface ectoderm cells formed dense membrane specializations, thus establishing a firm contact. The initial contact in the mesencephalon was formed by extensions from the surface ectoderm and was followed by the formation of specialized membrane junctions, as seen between the surface ectoderm in the rhombencephalon. The neuroepithelial cells facing the gap between the neural walls with their apical ends made contact with the cells from the opposing wall by numerous finger-like projections but membrane specializations failed to develop. The closing mechanism in the prosencephalon and anterior neuropore regions differed from the previous areas in that the initial contact was established by the neuroepithelial cells. Only after this contact had been formed did the surface ectoderm cells close the gap. In contrast with the other areas many phagocytosed particles were seen in the prosencephalon and in the region of the anterior neuropore. Many particles from degenerated cells were found inside healthy surrounding cells. Some of these particles contained nuclear material and cytoplasmic organelles.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00315716
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  • 5
    Electronic Resource
    Electronic Resource
    Lipofuscin in the cerebellar cortex of albino rats: An electron microscopic study (1979)
    Springer
    Anatomy and embryology 155 (1979), S. 333-345 
    Details
    ISSN: 1432-0568
    Keywords: Lipofuscin ; Cerebellar cortex ; Ultrastructure ; Senescent rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The ultrastructure of autofluorescent, PAS-positive lipofuscin in Purkinje, granule, Golgi epithelial, basket and stellate, microglial and perivascular cells in the cerebellar cortex of senescent rats is described. The membrane-bounded pigment is composed of three elements: 1) electron-lucent homogeneous droplets, 2) a granular matrix and 3) intensely osmiophilic patches. The proportions of these three components vary between cell types and one can grossly differentiate a neuronal and a glial lipofuscin. The lipofuscin granules of stellate and perivscular cells are different from lipofuscin of other cerebellar neurons and glia. It can be concluded from these morphological observations that each cerebellar cell type has its distinct lipofuscin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00317646
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  • 6
    Electronic Resource
    Electronic Resource
    In vitro studies on the self-differentiating capacities of quail adenohypophysis epithelium (1979)
    Springer
    Anatomy and embryology 156 (1979), S. 255-267 
    Details
    ISSN: 1432-0568
    Keywords: Adenohypophyseal rudiment ; Self-differentiation ; Ultrastructure ; Quail
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Isolated epithelial rudiments of 3–4 days quail embryo adenohypophysis were cultivated in vitro. Differentiation of glandular cells occured when culture conditions allowed the formation of explants characterized by the presence of a fibroblastic sheet and of an epithelial roof surrounding a central cavity. Differentiation did not occur when culture conditions did not allow the fibroblastic sheet to be established or resulted in the absence of a cavity. The importance of the explant structure in differentiation is also indicated by the location of glandular cells. In vitro they differentiate near the cavity derived from Rathke's pouch lumen while in situ they first appear at the periphery of the rudiment, near the basement membrane. The results of this study show that the adenohypophyseal primordium can differentiate without any mesenchymal influence. The observed differentiation seems to be correlated with fibroblast-secreted material, and this hypo-thesis is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00299626
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  • 7
    Electronic Resource
    Electronic Resource
    Barriers and transport properties of the perineurium (1979)
    Springer
    Acta neuropathologica 47 (1979), S. 139-143 
    Details
    ISSN: 1432-0533
    Keywords: Perineurium ; Permeability ; Peripheral nerve ; Rat ; Ultrastructure ; Autoradiography ; Albumin ; Peroxidase ; Protein deprivation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The permeability properties of the perineurium in sciatic nerves of 12-week-old rats were studied. The penetration of125I-labeled albumin and horseradish peroxidase into the perineurium was investigated electronmicroscopically 10, 30, and 120 min after the local extraneural application of the tracers. The autoradiographic study included age-matched protein-deprived rats. It was concluded that the perineurium acted as a diffusion barrier but also permitted a slow passage of the macromolecules into the endoneurium. The result indicates that this penetration to some extent is due to vesicular transport across the perineurial cells. The significance of these barrier and transport properties of the perineurium is discussed. No obvious differences in perineurial permeability between normal and protein-deprived rats were obtained.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00717037
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  • 8
    Electronic Resource
    Electronic Resource
    Pituitary adenomas secreting more than two hormones (1979)
    Springer
    Acta neuropathologica 48 (1979), S. 67-72 
    Details
    ISSN: 1432-0533
    Keywords: Pituitary adenoma ; Ultrastructure ; Classitication
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A case of pituitary adenoma secreting more than two hormones is presented. Morphological findings of this tumor have been correlated with biological behavior and with hormonal activity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00691794
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  • 9
    Electronic Resource
    Electronic Resource
    Melanosis Cerebelli (1979)
    Springer
    Acta neuropathologica 46 (1979), S. 215-220 
    Details
    ISSN: 1432-0533
    Keywords: Melanosis of the dentate nucleus ; Glial melanin ; Ultrastructure ; Intrared spectroscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The pigment present in melanosis cerebelli where it is deposited in the dentate nucleus and within the cerebellar granular layer is similar to that of S. nigra as judged from the typical infrared absorption bands near wave numbers 3.450 cm−1 and 1.650 cm−1, respectively. According to electron microscopic findings, this type of melanin arises within the astrocytes of the dentate nucleus. The astrocytic melanosomes exhibit a relatively monotonous substructure reaching diameters of 30 μ and more, and they differ in this respect from the triadic neuronal melanosomes of S. nigra that are strongly reminiscent of lipofuscin granules. These differences may be due to additional components of melanosomes, apparently specific of a particular cell type, and they are in turn accounted for by the dissimilar cellular metabolic make-up of nigral cells and astrocytes, respectively. This does not dismiss the possibility, however, that the melanin pigments of either type of melanosomes are the same or closely related to each other as far as their basic chemical composition is concerned. The cause of the melanosis of the dentate nucleus is not known. Possibly involutional tissue changes governed by endocrine factors among others may play an important part.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00690847
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  • 10
    Electronic Resource
    Electronic Resource
    Becker's X-linked muscular dystrophy histological, enzyme-histochemical, and ultrastructural studies of two cases, originally reported by Becker (1979)
    Springer
    Acta neuropathologica 46 (1979), S. 69-77 
    Details
    ISSN: 1432-0533
    Keywords: Muscular dystrophy ; Becker-type ; Benign X-linked ; Histopathology ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Muscle biopsies of two patients originally reported in the Göttingen family by Becker (1962) that formed the basis of separating a benign X-linked muscular dystrophy from the rapidly progressive Duchenne-type X-linked muscular dystrophy, revealed mild pathological changes in the younger patient and more advanced in the older one, consisting of increased spectra of fiber diameters, endomysial fibrosis, angulated fibers, pyknotic nuclear clumps and small groups of atrophic fibers. Essentially, both biopsies showed the same changes, but of different severity, possibly due to the differences in age and muscle biopsy sites. These changes were regarded “myopathic”, but a neurogenic component was suggested. Our observations accord well with those of a larger series (Bradley et al., 1978) where both electromyography and histopathology revealed a mixed “myopathic-neurogenic pattern” in patients with Becker-type dystrophy. Differential diagnostic aspects encompass Duchenne's muscular dystrophy, the other hereditary dystrophies and X-linked proximal spinal muscular atrophies. The precise nature of Becker-type muscular dystrophy requires morphological data on peripheral nerves, spinal roots and spinal cord anterior horn cells as well as sequential biopsy analysis to substantiate the primary site of pathology. However, on the basis of available data, it seems reasonable to suggest that the early changes of degeneration/regeneration which are accompanied by a markedly elevated CPK eventuate in the histopathologic and electromyographic patterns illustrated in these two patients with Beckertype dystrophy.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00684807
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  • 11
    Electronic Resource
    Electronic Resource
    An ultrastructural study of the cerebrospinal fluid in visna (1979)
    Springer
    Acta neuropathologica 48 (1979), S. 39-43 
    Details
    ISSN: 1432-0533
    Keywords: Visna ; Slow viral infection ; CSF ; Ultrastructure ; Myelin fractions
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary An electron microscopic examination was done on 8 samples of cerebrospinal fluid (CSF) from Icelandic sheep infected by the intracerebral route with visna virus. The specimens were collected 1 month, 2 months, and 4 years after infection. A differential cell count done on low-power electron micrographs showed that the cellular exudate was composed of mononuclear cells mainly macrophages and lymphocytes with a few plasma cells. Macrophages were with one exception more numerous than lymphocytes and an increased proportion of macrophages showed evidence of phagocytosis with time after infection. Reactive lymphocytes were in general more numerous than small lymphocytes. Various stages in the maturation of plasma cells were observed. The cellular composition in the CSF is compatible with the view that visna is an immunopathological process. Myelin figures and fragments of myelinated axons were observed in two specimens indicating an active myelin-breakdown. The possibility that escape of myelin into the CSF may lead to sensitization to myelin antigens and perpetuation of this chronic neurologic affection is discussed. Visna virions could not be demonstrated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00691789
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  • 12
    Electronic Resource
    Electronic Resource
    Neuronal degeneration in the brain of the brindled mouse (1979)
    Springer
    Acta neuropathologica 45 (1979), S. 17-25 
    Details
    ISSN: 1432-0533
    Keywords: Brindled mutant mouse ; Male hemizygotes ; Neuronal degeneration ; Ultrastructure ; Giant mitochondria ; Copper deficiency
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The brindled mouse (Mobr) is a neurological mutant mouse with clinical and biochemical features closely similar to Kinky hair syndrome (KHS) in humans. Neuronal degeneration in the cerebral cortex and thalamic nuclei was the constant neuropathological lesions in the CNS of the male hemizygotes of this mutant (Yajima and Suzuki, 1978). Ultrastructurally, many cortical neurons contained enlarged mitochondria with prominent tubular or vesicular cristae, which were similar to those described in the Purkinje cells in the human KHS (Ghatak et al., 1972) and in the rat brain with copper deficiency (Prohaska and Wells, 1975). Such mitochondria were observed not only in the degenerating neurons but even in the otherwise normal-appearing cortical neurons, suggesting that the mitochondrial damage possibly related to the deficient activities of the copper containing enzymes (cytochrome oxidase, etc.) preceded the neuronal degeneration. Many mitochondria in the severely degenerated neurons contained numerous electron dense spicules of possible calcium. Although rare, similar morphological alteration of neuronal mitochondria was also noted in the female heterozygotes, indicating the presence of possible subclinical defect in copper transport in the heterozygotes as well.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00691800
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  • 13
    Electronic Resource
    Electronic Resource
    Primary rhabdomyosarcoma of the cerebrum (1979)
    Springer
    Acta neuropathologica 45 (1979), S. 111-115 
    Details
    ISSN: 1432-0533
    Keywords: Brain tumor ; Rhabdomyosarcoma ; Mesenchymal cell ; Myotube ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A case of primary cerebral rhabdomyosarcoma in a 51-year-old female is reported. The histogenesis of this tumor is discussed. The clinicopathological features of 10 previously reported similar tumors of the CNS are briefly reviewed. Histologically the tumor was polymorphic, but composed of poorly differentiated cells interpreted as rhabdomyoblasts without definite cross-striation. Electron microscopy established that the poorly differentiated cells were of rhabdomyosarcomatous nature, compatible with presumptive myoblasts and analogous to developing fetal muscle.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00691888
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  • 14
    Electronic Resource
    Electronic Resource
    An ultrastructural study of cultured human meningioma cells (1979)
    Springer
    Acta neuropathologica 46 (1979), S. 11-15 
    Details
    ISSN: 1432-0533
    Keywords: Meningioma ; Cell culture ; Ultrastructure ; Cell junctions
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Ultrastructural alterations in human meningioma cells grown in vitro are reported. In early passages the cells retain some of the characteristic features of the original tumors. These include interdigitations associated with intercellular junctional devices (e.g., desmosomes, gap junctions). However, with repeated subculture these features tend to be less frequent. Typical whorl formations are observed only in primary cultures. The nunber of cytoplasmic filaments, lipid inclusions and other dense bodies increases with time in culture. Cytoplasmic invaginations into nuclei and the appearance of very large cells become more frequent in repeatedly transferred cultures.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00684798
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  • 15
    Electronic Resource
    Electronic Resource
    Studies on the early changes in acute isoniazid neuropathy in the rat (1979)
    Springer
    Acta neuropathologica 47 (1979), S. 85-92 
    Details
    ISSN: 1432-0533
    Keywords: Isoniazid ; Neuropathy ; Intoxication ; Dying back ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Large single doses of isoniazid by mouth (1–2 g/kg) have been shown to produce in rats Wallerian degeneration visible with the light microscope from the third day onwards. By contrast, changes in axons are seen from 24 h onwards by electron microscopy. The earliest ultrastructural changes are associated with vacuoles appearing between axon and Schwann cells. These are large and focal, and often compress the axon. The adjacent axon may show changes in smooth ER, and in microtubular arrangement. Alterations in smooth membranes and in mitochondria are visible in Schwann cell cytoplasm, not necessarily related to the vacuole formation and axonal features. It is suggested that INH neuropathy is essentially a multifocal axonal lesion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00717029
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  • 16
    Electronic Resource
    Electronic Resource
    Neurotoxicity of sodium cyanate (1979)
    Springer
    Acta neuropathologica 47 (1979), S. 75-79 
    Details
    ISSN: 1432-0533
    Keywords: Neurotoxicity ; Cyanate ; Demyelination ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The effects of sodium cyanate (NaNCO) on the nervous system of Maccaca nemestrina were studied at 2, 4, and 6 months of administration of the drug. The two groups injected with daily doses of 35 and 25 mg/kg/day of Na-cyanate developed a predominantly demyelinating lesion in the pyramidal tracts of the spinal cord. No neuronal changes were observed in the motor cortex, basal ganglia, midbrain, medulla or anterior horn cells of the spinal cord. There was no evidence of peripheral neuropathy. A comparison between the cyanate induced neuropathy in the rat and in the primate was drawn. Ultrastructurally, both species developed a demyelinating process of central or peripheral myelin characterized by vacuolation of the myelin sheath, removal of myelin debris by macrophages and re-myelination. There was little evidence of axoplasmic damage except for an occasional distended fiber containing abundant dense bodies and whorls of neurofilaments. Oligodendrocytes and Schwann cells were electron microscopically intact and participated actively in remyelination. Maccacas maintained at 15 mg/day and sham animals remained normal clinically and anatomically. The predominantly myelinotoxic effect of cyanate is similar to that produced by other myelinotoxic agents and is attributed to a selective modification of myelin proteins by carbamylation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00698277
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  • 17
    Electronic Resource
    Electronic Resource
    Ultrastructural study of medullomyoblastoma (1979)
    Springer
    Acta neuropathologica 48 (1979), S. 211-214 
    Details
    ISSN: 1432-0533
    Keywords: Medullomyoblastoma ; Desmoplastic medulloblastoma ; Teratoid ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A case of cerebellar medullomyoblastoma in a young boy was investigated by electron microscopy. The neuroectodermal component shows the characteristics of a desmoplastic medulloblastoma. The mesodermal component consists of more or less differentiated cross-striated muscle cells. Undifferentiated muscle cells are very similar to proliferated endothelial cells of blood vessels within the muscular component, so that an origin of this component from pluripotential endothelial cells of the vessel wall is suggested. This tumor is considered a malignant teratoid because of the derivation from two blastodermic layers and because of the midline localization in children suggesting a malformative origin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00690521
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  • 18
    Electronic Resource
    Electronic Resource
    Über Melanin und Melanosomen im ZNS im Vergleich zu extracerebralen Erscheinungsformen und synthetischem Melanin aus Dopamin und Serotonin (1979)
    Springer
    Acta neuropathologica 48 (1979), S. 177-188 
    Details
    ISSN: 1432-0533
    Keywords: Melanin ; Melanosomes ; Ultrastructure ; Infrared spectrophotometry ; X-ray diffraction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Melanosomes and isolated melanosomal fragments (melanin particles) originating from gangliocytes (substantia nigra), astroglia (melanosis cerebelli), and melanocytes (melanotic meningeoma; metastases of melanoblastoma; melanosis thalami of the goat) were compared with synthetic melanins prepared from dopamine and serotonin, respectively. Samples were examined by electron microscopy, X-ray diffraction analysis according to Debye-Scherrer and by infrared spectrophotometry, and the results were evaluated with regard to characteristic features as they may relate to specific cell types or chemical structures. On electron microscopy all three types of melanosomes could be differentiated unequivocally as could the two synthetic melanins. Thus, there were similarities between synthetic melanin from dopamine and the gliogenic melanins of the cerebellum; the synthetic melanin from serotonin resembled melanin of melanocytes. X-ray diffraction analysis yielded 2-4 Debye diffraction rings with all human and synthetic samples, suggesting short range orders between 3.8 to 5 Å the sample obtained from a goat with thalamic melanosis showed a specific reflex pattern. While diffraction patterns of some melanins were partially identical, in particular that of melanin from dopamine and melanin of substantia nigra and dentate nucleus, respectively, they were different for the various melanocytic melanins. Further investigations are required to determine whether these differences are due to disparities in basic chemical structures or conformations or else, to particular compositional features of the various types of melanocytes as they arise from benign or malignant tumors or a specific species. Infrared spectrophotometry at higher wave numbers revealed the well known patterns of melanins, which are not, however, very suitable, for their further differentiation. At lower wave numbers (‘fingerprinting’) melanin of substantia nigra and the glial melanin in melanosis cerebelli yielded additional absorption bands of identical configuration. In contrast to melanin from dopamine, melanin from serotonin exhibited a closely similar absorption pattern in this spectral range, suggesting that the neuroectodermal melanins may contain a component possibly arising from serotonin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00690518
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  • 19
    Electronic Resource
    Electronic Resource
    Tight junctions in choroid plexus papillomas (1979)
    Springer
    Acta neuropathologica 45 (1979), S. 159-160 
    Details
    ISSN: 1432-0533
    Keywords: Tight junction ; Choroid plexus papilloma ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Four cases of choroid plexus papilloma (CPP) obtained at the time of surgical excision were examined by electron microscopy and compared with normal choroid plexus (CP) of mouse and chick. In apical tight junctions fusion of the two outer leaflets of the adjacent cytoplasmic membrane was verified as in CP. This fact suggests that there is a blood-CSF barrier not only in CP but also in CPP.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00691895
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  • 20
    Electronic Resource
    Electronic Resource
    Ultrastructure of neurofibrillary tangles in progressive supranuclear palsy (1979)
    Springer
    Acta neuropathologica 48 (1979), S. 27-30 
    Details
    ISSN: 1432-0533
    Keywords: Progressive supranuclear palsy ; Neurofibrillary tangles ; Ultrastructure ; Straight tubule ; Twisted tubule
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The fine structure of neurofibrillary tangles in the hippocampal gyrus, substantia nigra, pontine nuclei and locus coeruleus of the brain was postmortem studied in a case of progressive supranuclear palsy. Straight tubules and twisted tubules were observed in both the cortical and subcortical neurofibrillary tangles. Most tubules appeared separately in each neuron but a few straight tubules were mixed with the twisted tubules in the cortical tangles. The implication and possible significance of this findings are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00691787
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  • 21
    Electronic Resource
    Electronic Resource
    Tumorigenic clones of hamster brain cells transformed in vitro by polyoma virus (1979)
    Springer
    Acta neuropathologica 48 (1979), S. 189-197 
    Details
    ISSN: 1432-0533
    Keywords: Hamster brain cells ; Polyoma virus ; Tumorigenicity ; Ultrastructure ; Specific nervous proteins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The morphological characteristics of three clones of hamster brain cells transformed in vitro by polyoma virus and of the tumors obtained after subcutaneous grafting of these cells in syngenic animals are reported. These clones appeared to be glial in nature by light and electron microscopy. The initial tumors induced by the three clones presented astrocytic features both by electron microscopy and immunohistochemistry. Analysis of the subsequent in vivo passages showed a decrease in cell differentiation, which was accompanied by a decrease in the latency period; after 2 years of serial transplantation, the tumors seemed poorly differentiated gliomas. A control cell line of hamster cerebellar cells evolved similarly.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00690519
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  • 22
    Electronic Resource
    Electronic Resource
    Capnocytophaga: New genus of Gram-negative gliding bacteria. II. Morphology and ultrastructure (1979)
    Springer
    Archives of microbiology 122 (1979), S. 17-27 
    Details
    ISSN: 1432-072X
    Keywords: Gliding bacteria ; CO2-requiring ; Periodontal disease ; Gram-negative ; Ultrastructure ; Capnocytophaga
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Gram-negative, anaerobic gliding bacteria were isolated from normal supragingival plaque and from periodontal lesions. Isolates could be divided into two size classes: small 2.4–4.2 μm×0.38–0.5 μm and large 4.8–5.8 μm×0.42–0.6 μm cells. The outer membrane was either loose-fitting and wavy, or taut, and of variable thickness. An electron-dense fuzz was discernible on several of the isolates. The periplasmic region was of variable electron-density. The genus Capnocytophaga has been proposed for these organisms based on morphological and cultural characteristics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00408041
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  • 23
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    Electronic Resource
    A comparative ultrastructural study of the ascospores of some Saccharomyces and Kluyveromyces species (1979)
    Springer
    Archives of microbiology 121 (1979), S. 53-59 
    Details
    ISSN: 1432-072X
    Keywords: Saccharomyces ; Kluyveromyces ; Ultrastructure ; Ascospore wall ; Taxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Three types of structure of the ascospore wall were found among the haploid Saccharomyces species examined: a warty wall (S. rouxii), a smooth wall with a single electron-light inner layer (S. bailii) and a smooth wall with a double light inner layer (S. montanus, S. florentinus). The latter type also occurred in Kluyveromyces thermotolerans and K. waltii. In K. fragilis spores the wall had a single light inner layer. The taxonomic implications of these findings were discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00409205
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  • 24
    Electronic Resource
    Electronic Resource
    Methanogenium, a new genus of marine methanogenic bacteria, and characterization ofMethanogenium cariaci sp. nov. andMethanogenium marisnigri sp. nov. (1979)
    Springer
    Archives of microbiology 121 (1979), S. 147-153 
    Details
    ISSN: 1432-072X
    Keywords: Methanogenium cariaci ; Methanogenium marisnigri ; Marine methanogenic bacteria ; Ultrastructure ; TaxonomyMethanogenium gen. nov.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A new genus of marine methanogenic bacteria and two species within this genus are described.Methanogenium is the proposed genus andMethanogenium cariaci the type species. Cells of the type species are Gram-negative, peritrichously flagellated, irregular cocci with a periodic wall surface pattern. Colonies formed by these bacteria are yellow, circular and umbonate with entire edges. The DNA base composition is 52 mol% guanine plus cytosine. Formate or hydrogen and carbon dioxide serve as substrates for growth. Cells ofMethanogenium marisnigri are of similar shape but smaller diameter thanM. cariaci. The colonies ofM. marisnigri are convex, and the DNA base composition is 61 mol % G+C. Formate or hydrogen and carbon dioxide are growth substrates. Sodium chloride is required for growth of both methanogens.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00689979
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  • 25
    Electronic Resource
    Electronic Resource
    The fine structure of neurones and cellular relationships in the abducens nucleus in the cat (1979)
    Springer
    Experimental brain research 35 (1979), S. 249-267 
    Details
    ISSN: 1432-1106
    Keywords: Abducens nucleus (cat) ; Peroxidase ; Neurones ; Synapses ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Light and electron microscope studies of the abducens nucleus in the cat have disclosed two populations of neurones: large neurones 25 to 75 μm in diameter and small neurones 10 to 25 μm in diameter. Characteristic of the large neurones are a highly developed granular endoplasmic reticulum and a large number of axo-somatic synapses. The small neurones have a poorly developed granular endoplasmic reticulum and comparative by few axo-somatic synapses. Injection of horseradish peroxidase (HRP) into the ipsilateral lateral rectus muscle has been used to identify abducens motoneurones which represent 65% of the total number of cells in the abducens nucleus and form part of the large cell population. The remaining unlabelled large neurones (30% of the large cell population) probably represent motoneurones of the retractor bulbi muscles, abducens motoneurones which have failed to transport the HRP, and possibly internuclear neurones. The small neurones (5% of the total cell population) were never labelled by HRP. Axo-somatic and axo-dendritic synapses are numerous. Many of the synaptic terminals form contacts with two or more different postsynaptic elements. Axo-axonic synapses and synapses on the initial segments of large neurones are also present. Nodal synapses and branching of myelinated axons are common findings. Finally, extended areas of close apposition between dendritic membranes are frequently observed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00236614
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  • 26
    Electronic Resource
    Electronic Resource
    Ultrastructural data, with special reference to bouton/glial relationships, from the hypoglossal nucleus after a second axotomy of the hypoglossal nerve (1979)
    Springer
    Experimental brain research 36 (1979), S. 107-118 
    Details
    ISSN: 1432-1106
    Keywords: Rat ; Hypoglossal nucleus ; Ultrastructure ; Second axotomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The left hypoglossal nerve of adult male albino rats was prevented from regenerating to the tongue after a distal axotomy by implanting the proximal stump into normally innervated left sternomastoid muscle. Eighty-four days after implantation, the hypoglossal nerve was transected again and its regeneration to the tongue unimpeded. From 8 to 70 days after this second axotomy the left hypoglossal nuclei were processed for quantitative ultrastructural analysis. The first aim of this study was to compare regeneration success in the hypoglossal nucleus after second axotomy with that accompanying outgrowth of the hypoglossal nerve into denervated sternomastoid muscle. During quantitative analysis a second aim developed, of elucidating bouton/glial relationships. The second axotomy induced loss and return of subsurface cisterns, dispersal and reassembly of Nissl substance, increase and decrease of microglial numbers, slight further loss and partial return of boutons with clear spherical vesicles and symmetrical synapses, slight increase and decrease of boutons with clear flat vesicles and symmetrical synapses, regrowth of retracted dendrites and restoration of their synapses, and gradual diminution of numbers of electron-dense neurones and dendrites. Astrocytes remained hypertrophied throughout. When compared with events in the hypoglossal nucleus accompanying innervation of denervated sternomastoid muscle by the hypoglossal nerve, the results suggest (1) that regeneration of the hypoglossal nerve to its own tongue muscle instead of to a foreign muscle caused no acceleration of recovery in the hypoglossal nucleus, and (2) that the microglial response is dependent on nerve integrity and not on bouton behaviour.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00238471
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  • 27
    Electronic Resource
    Electronic Resource
    Ultrastructural evidence of myofibroblasts in pseudomalignant myositis ossificans (1979)
    Springer
    Virchows Archiv 381 (1979), S. 189-203 
    Details
    ISSN: 1432-2307
    Keywords: Pseudomalignant ossifying myositis ; Ultrastructure ; Myofibroblasts ; Macrophages ; Pathogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Seven cases of pseudomalignant ossifying myositis with a typical clinical symptomatology have been reported. None of the cases had experienced an injury. All the lesions were intramuscular and all of them showed a zonal arrangement. Electron microscopy in three cases allowed the demonstration of cells showing morphological features of myofibroblasts and monocytic cells of the macrophage type. These previously unreported features together with the zonal pattern of the lesions indicate their reparative nature.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01257884
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  • 28
    Electronic Resource
    Electronic Resource
    Muscle spindles in rheumatoid arthritis (1979)
    Springer
    Virchows Archiv 382 (1979), S. 191-200 
    Details
    ISSN: 1432-2307
    Keywords: Rheumatoid arthritis ; Muscle spindles ; Neuromuscular spindles ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Ultrastructural features of muscle spindles were studied in biopsy material from 100 patients suffering from classical rheumatoid arthritis. Thickening of the outer capsule, increased amount of extracellular ground substance within the inner capsule, and marked thickening of the basement membrane of capillary blood vessels supplying the muscle spindles were observed. Chronic inflammatory cells and macrophages were present within the spindles. Changes affecting the intrafusal muscle fibres were also seen. They were manifest as atrophy and degeneration of the intrafusal muscle fibres, absence of the specialised junctional complexes, and of the intercellular bridges, microladders and satellite cells. It is suggested that the changes affecting the intrafusal muscle fibres are probably secondary. Damage to the myelinated nerves was present, while the sensory and motor nerve endings were well preserved.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01102874
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  • 29
    Electronic Resource
    Electronic Resource
    Ultrastructure of clear cell carcinoma of the ovary (1979)
    Springer
    Virchows Archiv 385 (1979), S. 77-91 
    Details
    ISSN: 1432-2307
    Keywords: Ultrastructure ; Ovary ; Clear cell carcinoma
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The fine structure of an ovarian clear cell carcinoma in a 65 year old woman was analysed. The tumor cells were of both clear and “hobnail” types. Both were characterized by the presence of short microvilli, abundant glycogen granules, a well-developed granular endoplasmic reticulum and Golgi apparatus, scanty lysosomes and very few lipid bodies. The tubules and gland-like spaces were always separated from the stroma by a basement membrane. At ultrastructural level the tumor cells do not resemble at all those of clear cell renal carcinomas. On the other hand, their submicroscopic features are strongly similar to those of the clear cell tumors found elsewhere in the female genital tract, emphasizing therewith their very probable müllerian origin. It is recommended to eliminate the term “mesonephroma ovarii” to designate the clear cell carcinomas of the ovary.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00433543
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  • 30
    Electronic Resource
    Electronic Resource
    Ultrastructural and histological study of 11 bronchial carcinoids (1979)
    Springer
    Virchows Archiv 381 (1979), S. 313-329 
    Details
    ISSN: 1432-2307
    Keywords: Bronchial carcinoids ; Different types ; Cells of origin ; Ultrastructure ; Histology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Seven of eleven bronchial carcinoids investigated showed cells with small granules resembling P cells which have already been described in human fetal and adult lung; two of these P cell tumours showed distinctive paraganglioid features. One tumour showed peculiar ultrastructural findings resembling closely those previously reported by Black (1969) in a so called “pulmonary oncocytoma”. Three remaining cases showed large secretory granules resembling those of type 3 cells already described by Hage (1973 b) in bronchial carcinoids; one of these tumours produced large amounts of 5-hydroxytryptamine (5HT). It is concluded that, on cytological grounds, at least two types of tumours can be distinguished among bronchial carcinoids, i.e. P cell and type 3 cell tumours. Moreover, two varieties of P cell carcinoids have been recognized, showing either the less frequent and more distinctive paraganglioid structure or the more common trabecular structure.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00432474
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  • 31
    Electronic Resource
    Electronic Resource
    Sclerosing angioma of the lung (1979)
    Springer
    Virchows Archiv 385 (1979), S. 93-101 
    Details
    ISSN: 1432-2307
    Keywords: Angioma ; Lung ; Carcinoma ; Pneumocytes ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Elektronenmikroskopische Untersuchung eines sog. sklerosierenden Hämangioms der Lunge, das im Oberlappen einer 32jährigen Frau zufällig entdeckt wurde. Der seltene gutartige Tumor, der lichtmikroskopisch wegen seines Gefäßreichtums an ein Hämangiom erinnert, zeigt elektronenmikroskopisch eine eindeutige epitheliale Struktur. Die Geschwulst entwickelt sich möglicherweise aus unreifen Pneumocyten. Histogenese sowie differential-diagnostische Probleme (Verwechslungsmöglichkeit mit Carcinom) werden diskutiert.
    Notes: Summary Electron microscopy of a sclerosing angioma of the lung, a coincidental finding in the upper lobe of a 32-year-old woman. The rare, benign tumor, whose vascular proliferation by light microscopy is reminiscent of an angioma, exhibits a clear epithelial structure by electron microscopy. The tumor may develop out of immature pneumocytes. The paper discusses histogenesis and problems of differential diagnosis (potential confusion with carcinomas).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00433544
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  • 32
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    Electronic Resource
    The ultrastructure of myxofibrosarcoma (1979)
    Springer
    Virchows Archiv 381 (1979), S. 121-139 
    Details
    ISSN: 1432-2307
    Keywords: Myxofibrosarcoma ; Fibroblast ; Histiocyte ; Myofibroblast ; Sarcoma ; Soft tissue tumour ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary An ultrastructural study of 11 myxofibrosarcomas is presented. The tumours were graded light-microscopically on a four-point scale according to cellularity, cell atypia and mitotic activity, as described in a previous paper: 3 were grade I, 2 grade II, 4 grade III and 2 grade IV. Fibroblast-like and myofibroblast-like cells predominated in the grade I and II tumours, while histiocyte-like cells were relatively few; in grade III and IV tumours histiocyte-like cells predominated. The fibroblast-like cells were spindle-shaped with fairly smoothly outlined nuclei, and contained abundant parallel membranes and wide cisternae of endoplasmic reticulum. The histocyte-like cells were irregular in shape and showed indented nuclei with large nucleoli and coarse, peripherally arranged clumps of heterochromatin. The cytoplasm of these cells showed prominent pseudopodiae, microvillous projections, conspicuous systems of vesicles and vacuoles, inclusions of lipid and pigment, numerous lysosomal structures and large digestive vacuoles indicating auto- and exophagocytosis. The myofibroblast-like cells showed abundance of parallelly arranged cytoplasmic microfilaments with dense body-like structures and condensation at the plasmic membrane. Occasional cells appeared to be intermediate forms and primitive looking, undifferentiated cells were also encountered. A few multinucleated tumours cells were seen in the grade IV tumours. Thus, the present study shows the composite fibroblastic, myofibroblastic and histiocytic character of the tumour cells of myxofibrosarcoma; the grade III and IV tumours, with a tendency to contain solid areas, seem to be closely related to the pleomorphic type of malignant fibrous histiocytoma.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01257879
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  • 33
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    Electronic Resource
    Giant cell tumor of bone (1979)
    Springer
    Virchows Archiv 381 (1979), S. 159-178 
    Details
    ISSN: 1432-2307
    Keywords: Giant cell tumor ; Ultrastructure ; Acid phosphatase ; Lysosomes ; Bone tumors
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Eleven benign giant cell tumors of bone were studied in the electron microscope, and the fine structural localization of acid phosphatase was elucidated. Three distinct cell types are always present in these tumors: stromal cells type 1; stromal cells type 2; and multinucleated giant cells. Small mononuclear cells may also occur, but are not likely to be actively participating in the neoplastic process. The range of variability in the fine structure of the different cell types constituting this tumor has been established. Variations in appearances include: a) presence of nuclear pseudoinclusions in stromal cells type 1 and multinucleated giant cells; b) aberrations in the structure of the rough surfaced endoplasmic reticulum in the same cell types; c) occurrence of ruffled borders, ectoplasmic layers and cytoplasmic labyrinths containing acid phosphatase in the giant cells. Some giant cells show evidence of marked phagocytic activity and contain large and numerous residual bodies carrying acid phosphatase. The significance of the interrelations between the different cell types are discussed and the possible role of stromal cells type 2 in immunological mechanisms directed against the tumor cells are mentioned.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01257881
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  • 34
    Electronic Resource
    Electronic Resource
    Functions of different receptor systems in the reptilian labyrinth (1979)
    Springer
    European archives of oto-rhino-laryngology and head & neck 224 (1979), S. 31-35 
    Details
    ISSN: 1434-4726
    Keywords: Intracellular potentials ; Ultrastructure ; Hair cells ; Labyrinth receptors ; Reptilia (Crocodilia, Gekkonidae)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Different biopotentials, following a mechanical stimulus, were recorded from single hair cells of the reptilian labyrinth, with electronoptically circumscript localizations: 1. From the apical cell pole, the receptor potential, intracellular or from the ciliary surface, within the physiological range proportional to stimulus amplitude, frequency or phase, without delay, and with no real threshold of mechanosensitivity, as measured by ciliary displacement amplitude or velocity. 2. From the synaptic zone, in the basal region of the hair cell, or from contacting nerve endings, the synaptic potentials, local excitatory or inhibitory processes, respectively, with measurable latencies and with non-linear distortion. 3. From the (dendritic) endings of the first afferent neuron (or neurons), spike-shaped action potentials, synchronized by the (excitatory) synaptic potentials. Characteristic curves were plotted as a quantitative representation of the mechano-electric input-output relations of the different types of hair cells. For proceeding morphological and physiological system analysis, the comparison of the different submammalian inner-ear receptor systems gives us some new possibilities of a closer correlation between ultrastructure and function.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00455221
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  • 35
    Electronic Resource
    Electronic Resource
    The autoimmune submandibular sialoadenitis of the NZB/NZW hybrid mice (1979)
    Springer
    European archives of oto-rhino-laryngology and head & neck 225 (1979), S. 57-65 
    Details
    ISSN: 1434-4726
    Keywords: NZB/NZW-Maus ; Ultrastruktur ; Submandibulare Speicheldrüse ; Lymphocyten ; Autoimmunes Leiden ; NZB/NZW mouse ; Ultrastructure ; Submandibular gland ; Lymphocytes ; Autoimmune disease
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary An electron microscopic study of the submandibular salivary gland lesion occurring in the NZB/NZW F1 hybrid mice was performed, and the findings were discussed and correlated to the autoimmune salivary gland disease observed in patients with Sjögren's syndrome. Within the submandibular salivary gland of this mouse strain focal infiltrates of mononuclear inflammatory cells were observed. Lymphoid cells were observed inside the basal lamina of acini and ducts, leading to destruction of these glandular units. The typical duct cell proliferation found in the autoimmune sialoadenitis of Sjögren's syndrome with the formation of the typical epimyoepithelial islands was not found in the submandibular glands of NZB/NZW mice.
    Notes: Zusammenfassung Eine elektronenmikroskopische Untersuchung der bei den NZB/NZW F1 hybriden Mäusen vorkommenden Veränderungen in den submandibularen Speicheldrüsen wurde durchgeführt, und die Befunde wurden diskutiert und korreliert mit dem autoimmunen Speicheldrüsenleiden, das bei Patienten mit Sjögrens-Syndrom beobachtet wird. In der submandibularen Speicheldrüse dieses Mäusestammes wurden begrenzte Infiltrationen mononuklearer inflammatorischer Zellen beobachtet. Die mononuklearen Zellen wurden hauptsächlich als lymphoide Zellen identifiziert. Außerdem wurden innerhalb der Lamina basilaris von Drüsen und Drüsengängen lymphoide Zellen beobachtet, die zur Zerstörung dieser Drüseneinheiten führen. Darüber hinaus wurde der typische Zuwachs der Drüsengangszellen, der bei der autoimmunen Speicheldrüsenentzündung des Sjögrens-Syndroms vorkommt und die Form von typischen epimyoepithelialen Inseln annimmt, in den submandibularen Drüsen der NZB/NZW-Mäuse nicht vorgefunden.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00455876
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  • 36
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    Electronic Resource
    Ultrastructural morphology of three-dimensional colonies of cells derived from a hepatocellular carcinoma (1979)
    Springer
    Journal of cancer research and clinical oncology 95 (1979), S. 225-232 
    Details
    ISSN: 1432-1335
    Keywords: Cultured tumour cells ; Ultrastructure ; 3-D Colonies
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Cultured hepatocellular carcinoma cells were studied during anchorage-independent growth in semi solid medium (Methocel). The regular occurrence of mitotic figures both at the surface and within the colonies precludes the possibility of such colonies being formed by re-aggregation. The estimated population doubling time in the three-dimensional (3-D) colonies is consistent with those two-dimensional of (2-D) colonies. Structures resembling bile canaliculi were observed between the closely opposed membranes from the well packed adjacent cells. Cell surface and ultrastructural features of the colonies and individual cells are presented and comparisons made with 2-D growth of normal and malignant liver cells in vitro. The formation of 3-D colonies may not only be an assay for transformed cells but also for predicting the type of tumors produced by re-innoculation of the in vitro transformed cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00410643
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  • 37
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    Electronic Resource
    Feinstrukturelle und mikroanalytische Untersuchungen an den Kristallen und Lithosomen des CiliatenEuplotes vannus (1979)
    Springer
    Protoplasma 99 (1979), S. 67-77 
    Details
    ISSN: 1615-6102
    Keywords: Ciliata ; Crystals ; Euplotes ; Lithosomes ; Microanalysis ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In the cytoplasm of the marine ciliateEuplotes vannus, there exist two conspicuous types of membrane bound inclusions: 1. irregularly shaped crystals which are highly anisotropic; 2. globular lithosomes characterized by concentrically arranged layers of deposits which exhibit only faint birefringence. Normally, both structures form distinct accumulations. Energy dispersive X-ray microanalysis of these accumulations reveals a high content of calcium and phosphorus, besides magnesium, sulphur and chlorine. Analysis of cell areas devoid of the inclusions show significantly lower calcium- and phosphorus-peaks.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01274070
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  • 38
    Electronic Resource
    Electronic Resource
    Studies on the biology of fish bone. III. Ultrastructure of osteogenesis and resorption in osteocytic (cellular) and anosteocytic (acellular) bones (1979)
    Springer
    Calcified tissue international 28 (1979), S. 43-56 
    Details
    ISSN: 1432-0827
    Keywords: Bone resorption ; Osteogenesis ; Fish bone ; Osteocytes ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary The comparative ultrastructure of fish bone osteogenesis and resorption induced by scale removal was described in the osteocytic (cellular-boned)Carassius auratus and the anosteocytic (acellular-boned)Tilapia macrocephala. Osteocytes, present in osteocytic bone, were lacking in anosteocytic bone. In osteocytic bone the osteoblast secreted a collagenous preosseous matrix in which it became enmeshed and then was termed a preosteocyte. When the preosseous matrix mineralized, the preosteocyte was termed an osteocyte and was completely surrounded by bone. In anosteocytic bone the osteoblasts receded from the mineralizing front and never became trapped as osteocytes. During resorption, types A and B resorptive cells, present in both bone types, invaded the matrix and demineralized the osseous zone. These cells were characterized by large amounts of granular endoplasmic reticulum and intracellular inclusions containing crystal-like material. Although functionally similar to mammalian osteoclasts, these cells lacked a characteristic ruffled border and were not multinucleated. The osteocytes of cellular bone did not appear to be involved during demineralization.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02441217
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  • 39
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    Electronic Resource
    Ultrastructure of skeletal muscle after CO2-laser incision (1979)
    Springer
    Research in experimental medicine 176 (1979), S. 69-79 
    Details
    ISSN: 1433-8580
    Keywords: Skeletal muscle ; CO2-laser ; Surgery ; Ultrastructure ; Rat ; Skelettmuskelfaser ; CO2-Laser ; Chirurgie ; Ultrastruktur ; Ratte
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Der Zustand der dorsalen Haut und des darunterliegenden Muskelgewebes der Ratte wurde unmittelbar nach CO2-Laser-Schnitten mit Hilfe von licht- und elektronenmikroskopischen Methoden untersucht. In der subkutanen Skelettmuskelschicht konnte eine erhöhte Empfindlichkeit des Gewebes festgestellt werden, die in einer ausgeprägten Abfolge von Zellveränderungen ihren Ausdruck findet. Diese Schädigungen werden hauptsächlich den thermischen Effekten der Laserstrahlung zugeschrieben. Die Bedeutung dieser Untersuchungsergebnisse in Hinblick auf die Verwendung von Laser in der Chirurgie wird besprochen.
    Notes: Summary The immediate effect of CO2-laser incision to the dorsal skin and underlying muscular tissue of rats was studied by light and electron microscopical methods. In the subcutaneous layer of skeletal muscle cells an increased susceptibility was found, resulting in distinct zones of cellular changes which are attributed mainly to thermal effects of the laser beam. The importance of these findings for the surgical application of lasers is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01852112
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  • 40
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    Electronic Resource
    Zur Pathogenese der calcifizierenden odontogenen Cyste (Gorlin-Cyste) (1979)
    Springer
    Virchows Archiv 384 (1979), S. 307-324 
    Details
    ISSN: 1432-2307
    Keywords: Calcifying odontogenic cyst ; Dysplastic dentin ; Hornified cells ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Four new cases of calcifying odontogenic cyst (COC) were studied by light- and electronmicroscope. The ages of the patients were between 20 and 68 years. Three cysts were located in the maxilla, the other was in the mandible. The history of two patients was followed up for five and ten years respectively. The longer the history the bigger were the cysts. All cysts showed histologically identical alterations of the epithelium with 1) a pronounced cylindrical basal cell layer, 2) cells resembling stellate reticulum, and 3) ghost cells. So called dysplastic dentin was laid down in the connective tissue only in the two cases with a long history. Ultrastructurally the odontogenic epithelium of all cysts showed keratinization similar to the epidermis. Two types of keratinization are known to occur in the epidermis, soft keratinization with keratohyalin granules in ortho- and parakeratosis, and hard keratinization without keratohyalin granules in nails (horn). Both types of keratinization occur in odontogenic epithelium. The COC is an example of hard keratinization. Ghost cells form prekeratin which in turn becomes horn and is found in areas of confluent ghost cells and in the so called dysplastic dentin. We conclude that the alteration of odontogenic epithelium in COC and in special odontogenic tumors is a secondary phenomenon resembling ortho- and parakeratosis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00428232
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  • 41
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    Electronic Resource
    The trabecular meshwork of a non-glaucomatous eye with the exfoliation syndrome (1979)
    Springer
    Virchows Archiv 384 (1979), S. 347-355 
    Details
    ISSN: 1432-2307
    Keywords: Exfoliation syndrome ; Non-glaucomatous eye ; Trabecular meshwork ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The trabecular meshwork of a non-glaucomatous eye with the exfoliation syndrome was investigated. The exfoliation material was found mainly in massive deposits in the subendothelial region of the outer and inner wall of Schlemm's canal, in the cribriform area and the uveal meshwork. The other alterations of the trabeculum corneosclerale corresponded to the age of the patient. The concept that the accumulation of exfoliation material is an important pathogenetic factor in the development of glaucoma was not confirmed by this study.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00428235
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  • 42
    Electronic Resource
    Electronic Resource
    Adrenal adenomata causing primary aldosteronism (1979)
    Springer
    Virchows Archiv 384 (1979), S. 93-102 
    Details
    ISSN: 1432-2307
    Keywords: Primary aldosteronism ; Adrenal adenomata ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Adenomata taken from 25 patients with primary aldosteronism were observed by electron microscopy. The cells in the adenoma had a well developed agranular endoplasmic reticulum but granular endoplasmic reticulum was not prominent. Most of the mitochondria resembled those in the cells of the zona glomerulosa, suggesting that the adenomata which caused primary aldosteronism are derived from this zone. Spironolactone bodies were found in the cells of the adenoma from a patient who received spironolactone. Their appearance was identical to that descrived in previous reports.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00427154
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  • 43
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    Electronic Resource
    Rat liver alterations after chronic treatment with hexachlorobenzene (1979)
    Springer
    Virchows Archiv 382 (1979), S. 127-137 
    Details
    ISSN: 1432-2307
    Keywords: Hexachlorobenzene ; Liver ; Ultrastructure ; Porphyrins ; Smooth endoplasmic reticulum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Groups of female rats were treated orally with 0.5, 2.0, 8.0, and 32 mg/kg hexachlorobenzene twice a week for 203 days. The liver content of hexachlorobenzene was found to be dose-dependent. In the animals treated with the highest dose the concentration was 273 μg/g hexachlorobenzene. In the fresh and fixed hepatic tissue of the treated animals pink fluorescence was observed. Electron microscopy revealed a dose dependent enlargement of all hepatocytes due to proliferation of the SER in the centrolobular area or to increased glycogen deposits (β- or α-particles) and SER in the intermediary and periportal area. Numerous porphyrin deposits and siderosomes, intimate disorganisation and moderate dislocation of the RER and a moderate enlargement of bizarre-shaped mitochondria were recognized. The relationship between porphyrin crystals and mitochondria on the one hand and between SER and glycogen deposits on the other is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01102869
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  • 44
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    Electronic Resource
    The fine structure of meningiomas: An attempted classification (1979)
    Springer
    Virchows Archiv 382 (1979), S. 201-216 
    Details
    ISSN: 1432-2307
    Keywords: Meningioma ; Ultrastructure ; Classification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary We examined 23 meningiomas by electron microscopy. In each case it was possible to distinguish certain cells with epithelial features (desmosomes, microfilaments, interdigitating extensions) and others with fibroblastic features (collagen fibers). Others cells of transitional form were also seen. The proportion of these cellular types is variable, making it possible to classify meningiomas into seven types progressing gradually from a purely epithelial type to a purely fibroblastic one. We found no important ultrastructural abnormalities in the cells. These case reports confirm the uniqueness of meningiomas, which are composed of variously shaped cells but have their origin from a single cellular type. This has double potentiality for fibroblastic and epithelial differentiation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01102875
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  • 45
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    Electronic Resource
    Ultrastructural observations on axonal swelling in the human gracile nucleus (1979)
    Springer
    Virchows Archiv 382 (1979), S. 217-226 
    Details
    ISSN: 1432-2307
    Keywords: Axonal swelling ; Spheroid ; Dystrophic axon ; Ultrastructure ; Human gracile nucleus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The morphology of the spheroids in human gracile nuclei was studied by light and electron microscopy. Various spheroids encountered in the present study could be classified into three types based on the internal structure: The first one was chiefly composed of many irregular homogeneous dense bodies, multivesicular bodies and mitochondria. The dense bodies seemed to deposit multicentrically in an axon in the early stage of “balloon” formation and coalesce to form larger ones. The second was characterized by a marked accumulation of closely approximated mitochondria and dense concentric bodies. In the third the most characteristic findings were neurofibrillary accumulation and aggregations of dense bodies. These findings showed some divergence from those of dystrophic axons and the last two mimic those of degenerative or regenerative axons, which suggested that axonal swelling (including dystrophic axon) is not characteristic reaction of specific disease but rather nonspecific one to a variety of noxious stimuli. With light microscope, it was difficult to distinguish balloons with different structures since they were quite diverse and manifold in their shape, size, appearance and stainability. Intra-axonal corpora amylacea were seen in most cases and their incidence appeared to be nonspecific for any diseases.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01102876
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  • 46
    Electronic Resource
    Electronic Resource
    Malignant giant cell tumor of tendon sheath (1979)
    Springer
    Virchows Archiv 382 (1979), S. 237-243 
    Details
    ISSN: 1432-2307
    Keywords: Giant cell tumors of tendon sheath ; Soft tissue tumors ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A benign, but aggressive, giant cell tumor of tendon sheath developed over a period of 20 years into a metastasizing, histologically malignant giant cell tumor. Ultrastructure of the malignant tumor showed the same five cell types as described in giant cell tumors of tendon sheath. Even the same crystals were identified in the osteoblast-like and osteoclast-like cells. It therefore appears reasonable to assume that giant cell tumors of tendon sheath indeed are neoplasms with a malignant potential and not an inflammatory reaction of synovial cells as previously suggested. Both the benign and malignant tumors seem to be of mesenchymal derivation with partial osseous differentiation. No ultrastructural similarities with fibrous histiocytoma were apparent.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01102878
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  • 47
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    Electronic Resource
    Zur Ultrastrukturpathologie der vorbehandelten Basedow-Struma (1979)
    Springer
    Virchows Archiv 383 (1979), S. 181-193 
    Details
    ISSN: 1432-2307
    Keywords: Graves' disease ; Preoperative treatment ; Ultrastructure ; Antithyroid drug treatment ; Lithium ; Cellular involution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary This electron microscopic study is based on 12 cases of toxic goiter treated preoperatively with iodide, thionamides and lithium. Essential features of Graves' disease include hypertrophy of the follicular epithelium, papillary follicular infoldings and frequently, lymphocytic infiltration and fibrosis. After preoperative therapy most thyroid glands show involutional changes of varying degree with reaccumulation of colloid and flattening of the follicular epithelium. In a small number of histologically examined cases (n=84) colloid goitre (13%) and nodular goitre (8%) were noted. Lymphocytic infiltration and interstitial fibrosis do not show any correlation with preoperative treatment. Ultrastructurally, different stages of cellular involution are seen. The hypertrophic follicular cell shows an increased cell surface with numerous long microvilli and some pseudopodia at the apical border and an occasionally thickened, basal lamina. The cytoplasm contains a well developed system of organelles which synthesizes (rough endoplasmic reticulum, Golgi apparatus, cytoplasmic vesicles) and degrades (colloid droplets, lysosomes) thyroglobulin. With increasing cellular atrophy we found cells to be reduced in size but to contain well developed thyroglobulin-synthesizing organelles. Finally, we observed completely atrophic flat cells with only a few organelles. There are at date no electron microscopic communications on lithiumpretreated thyroid glands. After lithium therapy characteristic changes are observed at the membranes of the cytocavitary system, which are often found to be thin and occasionally to be damaged. The rough endoplasmic reticulum is virtually deprived of ribosomes and the Golgi apparatus seems to be underdeveloped. The number of apical microvilli is reduced. The morphological findings following iodide-, thionamide- and lithium pretreatment in Graves' disease are discussed in light of recent biochemical results.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01200898
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  • 48
    Electronic Resource
    Electronic Resource
    Das Lymphsystem des Kehlkopfes (1979)
    Springer
    European archives of oto-rhino-laryngology and head & neck 225 (1979), S. 165-180 
    Details
    ISSN: 1434-4726
    Keywords: Lymphatics ; Larynx ; Lymphangioscopy ; Ultrastructure ; Lymphgefäße ; Kehlkopf ; Lymphangioskopie ; Ultrastruktur
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Die Lymphdrainage des menschlichen Kehlkopfes wurde an autoptischem und in vivo Material lymphangioskopisch registriert. Auf ultrastruktureller Ebene erfolgte die Untersuchung des morphologischen Aufbaus von Lymphkapillaren und Lymphsammelgefäßen. Im Elektronenmikroskop zeigt der Wandaufbau der Lymphgefäße des Kehlkopfes nur geringgradige Unterschiede zum Lymphgefäßsystem anderer Körperregionen. Diese Unterschiede finden sich vor allem im Wandklappenaufbau der Lymphsammelgefäße. Eine strenge Kompartmentierung einzelner Kehlkopfregionen läßt sich nicht nachweisen. Ausnahme bilden die Lymphkapillaren des freien Randes der Stimmlippe. Anastomosen der Lymphkapillaren und -sammelgefäße erlauben Mittelinien- und Kehlkopfetagen-überschreitende Lymphdrainagen. Diese bestehenden Kollateralabflüsse sind bei Behinderung der physiologischen Lymphdrainage von klinischer Bedeutung.
    Notes: Summary From post mortem and in vivo tissues, the lymphatic drainage of the human larynx has been evaluated by means of lymphangioscopy. The fine structure of lymphatic capillaries and collecting vessels was examined in an electron microscope study. At the ultrastructural level, the structural components of the laryngeal lymphatic vascular wall showed only minor differences when compared to other lymphatic tissues. These differences concerned the valvular morphology of the lymphatic collecting vessels. There was no evidence of specific lymphatic drainage for distinct laryngeal areas, with the exception of the lymphatic capillaries of the free margin of the vocal cords. On account of anastomoses of lymphatic capillaries and collecting vessels lymphatic drainage could cross over both the laryngeal midline and other laryngeal levels. In pathological conditions with impediment of lymphatic flow, these collateral communications are of clinical significance.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00455251
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  • 49
    Electronic Resource
    Electronic Resource
    Quantitative analysis of lymphoid cells in cutaneous low-grade malignant B-cell lymphomas (1979)
    Springer
    Archives of dermatological research 266 (1979), S. 239-251 
    Details
    ISSN: 1432-069X
    Keywords: Cutaneous malignant B-cell lymphoma ; Ultrastructure ; Semithin sections ; Cutane maligne B-Zell Lymphome ; Ultrastruktur ; Semidünnschnitte
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Achtzehn Fälle von cutanen B-Zell Lymphomen von niedrigem Malignitätsgrad (gesichert durch Enzymcytochemie und Immuncytologie) wurden morphologisch mittels Semidünn- und Dünnschnitten unter-sucht. Vier Typen mit einem charakteristischen Muster von verschiedenen lymphoiden Zellen wurden gefunden: (1) Der lymphocytische Typ, überwiegend aus kleinen Lymphocyten bestehend; (2) der lymphocytoide (zentrocytische oder prolymphocytische) Typ, bei dem neben kleinen auch mittelgroße Lymphocyten (mit und ohne geknitterten Zellkernen) erscheinen; (3) der lymphoplasmacytoide Typ, bei dem neben kleinen und mittelgroßen Lymphocyten lymphoide Zellen mit unterschiedlich ausgeprägten Differenzierungsmerkmalen von Plasmazellen zu finden sind; sowie (4) der zentroblastische-zentrocytische Typ. Hier sind neben kleinen und mittelgroßen Lymphocyten auch Zentroblasten zu beobachten. Insgesamt stimmen die hier erhobenen morphologischen Befunde gut mit entsprechenden nodalen malignen Lymphomen überein. Die Anwendung moderner Klassifikationen von non-Hodgkin Lymphomen, insbesondere die »Kiel-Klassifikation«, erscheint daher auch für die Hautlymphome gerechtfertigt. Dünn- und Semidünnschnitte eignen sich bei der Routinediagnostik von derartigen Krankheitsfällen, vor allem dann, wenn mit Paraffinschnitten keine klare diagnostische Einordnung erfolgen konnte.
    Notes: Summary Eighteen cases of low grade malignant B-cell lymphomas (confirmed through enzyme cytochemistry and immunocytology) were investigated morphologically using thin and semithin sections. Four types with a characteristic pattern of different lymphoid cells were found: (1) the lymphocytic type, predominantly consisting of small lymphocytes; (2) the lymphocytoid (centrocytic or prolymphocytic) type, in which in addition to small lymphocytes medium-sized lymphocytes (with or without cleaved nuclei) are found; (3) the lymphoplasmacytoid type, where lymphoid cells with different degrees of similarity to plasma cells are seen as well as small and medium-sized lymphocytes; and (4) the centroblastic-centrocytic type, in which centroblasts are present next to small and medium-sized lymphocytes. These findings correlate well with the morphological observations in low-grade malignant B-cell lymphomas of the lymph nodes. Therefore, the application of modern classifications of non-Hodgkin lymphomas, such as the “Kiel Classification”, to cutaneous lymphomas appears justified. Thin and semithin sections are particularly useful in suspected cases of cutaneous malignant lymphoma, in which paraffin sections did not allow the diagnosis to be established.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00418570
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  • 50
    Electronic Resource
    Electronic Resource
    A specific ultrastructural marker for disseminated lipogranulomatosis (Farber) (1979)
    Springer
    Archives of dermatological research 266 (1979), S. 187-196 
    Details
    ISSN: 1432-069X
    Keywords: Disseminated lipogranulomatosis ; Farber's disease ; Sphingolipidoses ; Ceramide ; Ultrastructure ; Disseminierte Lipogranulomatose ; M. Farber ; Sphingolipidosen ; Ceramid ; Ultrastruktur
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Eine elektronenmikroskopische Untersuchung von 2 Hautläsionen bei einem aus der Türkei stammenden 2jährigen Jungen mit disseminierter Lipogranulomatosis (Farber) zeigte curvilineare Körper in Fibroblasten, Histiocyten und Endothelzellen, flache Membrananordnungen in Fibroblasten und Endothelzellen und spindelartige Körper in Schwann-Zellen. In peripheren Lymphocyten fanden sich nur Veränderungen der Mitochondrien (Schwellung und aufgebrochene Cristae), jedoch keine Einschlußkörper. Die intracytoplasmatischen curvilinearen Körper waren zahlreich und leicht nachweisbar. Sie scheinen für den M. Farber spezifisch zu sein, und es wird vorgeschlagen, sie “Farber Körper” zu nennen. Die Diagnose dieser Ceramidspeicherkrankheit, in der das histologische Bild weitgehend unspezifisch ist, kann daher ultrastrukturell bestätigt werden.
    Notes: Summary An ultrastructural investigation of two cutaneous lesions in a two-year-old Turkish boy with disseminated lipogranulomatosis (Farber) revealed curvilinear bodies in fibroblasts, histiocytes, and endothelial cells; “elongated membranes” in fibroblasts and endothelial cells; “zebra bodies” in endothelial cells; and spindle-shaped bodies in Schwann cells. In peripheral lymphocytes only alterations of mitochondria (swelling and ruptured cristae) but no inclusion bodies were found. Curvilinear bodies were numerous and easily identifiable; they appear to be characteristic of Farber's disease, and naming them “Farber bodies” is proposed. The diagnosis of this ceramide storage disease, in which the histological examination is relatively unspecific, can therefore be confirmed ultrastructurally.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00694628
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  • 51
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    Electronic Resource
    Quantitative analysis of lymphoid cells in myocosis fungoides, Sézary's syndrome and parapsoriasis en plaques (1979)
    Springer
    Archives of dermatological research 264 (1979), S. 17-28 
    Details
    ISSN: 1432-069X
    Keywords: Malignant lymphoma ; Mycosis fungoides ; Sezary's syndrome ; Parapsoriasis en plaques ; Ultrastructure ; Semi-thin sections ; Maligne Lymphome ; Mykosis fungoides ; Sézary-Syndrom ; Parapsoriasis en plaques ; Ultrastruktur ; Semi-Dünnschnitt-Technik
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Semi-Dünnschnitte erlauben eine bessere morphologische Differenzierung von lymphoiden Zellen als Paraffinschnitte. Zur Prüfung ihrer möglichen Bedeutung bei der Diagnostik von kutanen T-Zell-Lymphomen wurden folgende Untersuchungen durchgeführt: Lymphoide Zellen im cellulären Infiltrat von Hautläsionen bei Mykosis fungoides (11 Fälle), Sézary-Syndrom (9 Fälle) und Parapsoriasis en plaques (14 Fälle) wurden mittels Semi-Dünnschnitten von Epon-eingebetteten Biopsien quantitativ analysiert. Als Kontrollen wurden Biopsien von Epikutan-Test-Reaktionen, Kontaktdermatitis, initialer Psoriasis und Erythema chronicum migrans untersucht. Eine hohe Prozentzahl von Lutzner-(Sézary)-Zellen fand sich ständig beim Sézary-Syndrom und gelegentlich bei der Parapsoriasis en plaques. Bei der Mycosis fungoides überwogen lymphocytoide Zellen mit helleren Kernen als bei normalen Lymphocyten. In den meisten Tumorläsionen sowohl bei der Mycosis fungoides als auch beim Sézary-Syndrom fand sich eine erhöhte Prozentzahl von Immunoblasten. Lymphocyten waren der häufigste Zelltyp bei der Parapsoriasis en plaques und bei den Kontrollen. Diese Befunde könnten nützlich für die Diagnostik von Problemfällen sein, für die die Semi-Dünnschnitt-Technik als eine weitere Methode empfohlen wird.
    Notes: Summary Semi-thin sections are superior to paraffin sections for differentiating lymphoid cells. To test their possible significance in the diagnosis of cutaneous T-cell lymphomas, the following investigation was made: A quantitative analysis of lymphoid cells in the cellular infiltrate of cutaneous lesions of mycosis fungoides (11 cases), Sézary's syndrome (9 cases), and parapsoriasis en plaques (14 cases) was made using semi-thin sections of eponembedded biopsies. As controls biopsies of patch test reactions, contact dermatitis, initial psoriasis, and erythema chronicum migrans were studied. A high percentage of Lutzner (Sézary) cells was consistently found in Sézary's syndrome and occasionally in parapsoriasis en plaques only. In mycosis fungoides lymphocytoid cells with the nuclei, less dense than in normal lymphocytes were predominant. In most tumorous lesions of mycosis fungoides and of Sézary's syndrome an increased percentage of immunoblasts was noted. Normal lymphocytes were the most frequent cell type in parapsoriasis en plaques as well as in the controls. These findings may prove helpful in the diagnosis of problem cases, for which the use of semi-thin sections is recommended.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00417275
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    Electronic Resource
    Ultrastructural aspects of normolipidemic xanthomatosis (1979)
    Springer
    Archives of dermatological research 266 (1979), S. 143-159 
    Details
    ISSN: 1432-069X
    Keywords: Xanthoma ; Ultrastructure ; Lipid vacuoles ; Myelin figures ; Xanthoma ; Ultrastruktur ; Lipidvacuolen ; Myelinfiguren
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Eine elektronenmikroskopische Untersuchung von 10 Patienten mit normolipämischer Xanthomatose und von 2 Patienten mit hyperlipoproteinämischer Xanthomatose Typ IV und V ergab folgende Befunde: In allen Fällen wurden große Mengen von Histiocyten mit intracytoplasmatischen Lipid-Vacuolen, Lysosomen und Myelinfiguren gesehen. Dazu waren Mikrofilamente und Lipid-Vacuolen in einigen Fibroblasten zu beobachten. In sämtlichen Präparaten fanden sich mehrkernige histiocytäre Riesenzellen mit kristallinen Spalträumen sowie einige Mastzellen. Lipid-Vacuolen konnten auch in Schwannzellen, Endothelzellen und in Pericyten nachgewiesen werden. In einigen Endothelzellen und Pericyten beobachtete man Duplikaturen der basalen vaskulären Laminae und “rob-shaped” tubuläre Körperchen. Beim Xanthelasma palpebrarum und dem Xanthoma planum disseminatum fanden sich die histiocytären Schaumzellen in perivaskulärer Anordnung, wie bei den hyperlipoproteinämischen Xanthomatosen. Wir folgern, daß die ultrastrukturellen Aspekte in den verschiedenen Xanthomatosen im wesentlichen gleich sind, zumal große Ablagerungen von Fett in allen Zellen zu sehen sind. In den normolipämischen Xanthomatosen (Xanthelasma palpebrarum und Xanthoma planum disseminatum) ist die Art der Entstehung von Fettablagerungen vermutlich die gleiche wie bei den hyperlipoproteinämischen Xanthomatosen; sie unterscheiden sich jedoch von den Fettablagerungen beim Xanthoma disseminatum und beim Xanthogranuloma juvenile. Eine Einteilung der normolipämischen Xanthomatosen wird vorgestellt.
    Notes: Summary Electron microscopic aspects in ten cases of normolipidemic cutaneous xanthomatosis have been investigated. Two additional types IV and V hyperlipoproteinaemic xanthomatosis have also been included. Ultrastructural findings in all cases were similar. Abundant histiocytic cells with numerous intracytoplasmic lipid vacuoles, lysosomes, and myelin-figures, were the striking features. Moreover, in older lesions microfilaments and lipid vacuoles were found in some fibroblastic cells, as well as long space collagen around them. In some specimens we observed: giant multinucleated histiocytic cells, crystalline cleft-like spaces in histiocytes and some mastocytes with lipidic crystals in the extracellular space, as well as lipid vacuoles in Schwann cells, endothelial cells and pericytes. Rod-shaped tubulated bodies were found in some endothelial cells, with multiple basal vascular laminae. In xantelasma palpebrarum and in disseminate plane xanthoma the histiocytary foamy cells adopted a perivascular arrangement, as in hyperlipoproteinemic xanthomatosis. We concluded that ultrastructural aspects of different xanthomatosis are fairly similar as a consequence of the large amount of intracytoplasmic lipids accumulated in xanthomatous cells. In xanthelasma palpebrarum and in disseminated plane xanthoma this cell phase is reached by similar pathways to those for hyperlipoproteinemic xanthomatosis, whilst in xanthoma disseminatum and juvenile xanthogranuloma the pathways seem to be different. A classification of normolipidemic xanthomatosis is also provided.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00694624
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  • 53
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    Electronic Resource
    Ultrastructural studies on early events in fertilization of the bivalveLaternula limicola (1979)
    Springer
    Protoplasma 100 (1979), S. 73-83 
    Details
    ISSN: 1615-6102
    Keywords: Bivalve ; Fertilization ; Laternula limicola ; Sperm-egg interaction ; Temporary-acrosome ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Ultrastructural studies on sperm-egg interaction at the time of fertilization inLaternula limicola were performed. The “temporary-acrosome” did not change morphologically while the sperm passed through the egg investments. At the onset of sperm entrance into the egg, however, the temporary-acrosome and mitochondria were eliminated from the sperm. Afterwards the sperm was engulfed by the egg surface without membrane fusion of the gametes. After entry the sperm nucleus was surrounded by four membranes: the plasma membranes of the egg and of the sperm, and the membranes of the sperm nuclear envelope. As the sperm nucleus differentiated into the male pronucleus, the plasma membranes of both the sperm and egg were initially vesiculated, then dispersed into the egg cytoplasm. Finally, the sperm nuclear envelope changed into the male pronuclear membrane accompanying sperm chromatin dispersion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01276302
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  • 54
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    Electronic Resource
    Ultrastructure of primary sporidia of a wheat-bunt fungus,Tilletia caries, during ontogeny and mating (1979)
    Springer
    Protoplasma 99 (1979), S. 189-202 
    Details
    ISSN: 1615-6102
    Keywords: Mating ; Sporidia ; Tilletia ; Ultrastructure ; Wheat bunt
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Primary sporidia ofTilletia caries (DC.) Tul. are borne on denticles at the tips of promycelia. The promycelia contain many small vacuoles and mitochondria and numerous lipid bodies. As the primary sporidia develop, the promycelial cytoplasm passes into the nascent cells. Septa develop between the bases of mature sporidia and the tips of the denticles. Sporidia that abscise from the denticles commonly have prominent birth scars at their bases. The sporidia have very thin walls, few vacuoles, attenuated mitochondria, and numerous lipid bodies. Conjugation pegs are generally produced by both members of a conjugating pair of sporidia and there are bud scars where they emerge from the sporidia. The sporidial walls are apparently hydrolyzed during emergence of the pegs. Vesicles are sometimes present at the tips of the conjugation pegs and, before fusion, electron-dense accumulations are sometimes observed between the tips of adjacent pegs. The approaching conjugation pegs are precisely aligned prior to fusion, suggesting polar communication. The walls of the conjugation pegs fuse and then are hydrolyzed. Fused sporidia are relatively homogeneous in content. The nucleus in a sporidum is often close to the conjugation tube and occasionally is partly within the fusion tube.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01275734
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    The effects of anaerobiosis and metabolic inhibitors on mitochondrial ultrastructure inAmoeba proteus (1979)
    Springer
    Protoplasma 99 (1979), S. 275-288 
    Details
    ISSN: 1615-6102
    Keywords: Amoeba proteus ; Anaerobiosis ; Metabolic inhibitors ; Mitochondria ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Anaerobiosis or exposure to the metabolic inhibitors potassium cyanide and Antimycin A produced changes in the form of living amoebae. These were accompanied by mitochondrial changes in fixed cells. Increasing the anaerobic period increased the percentage of mitochondria affected and resulted in a gradual reduction and eventual loss of the condensed Type I mitochondria ofAmoeba proteus. The rounder Type II mitochondria were not lost but underwent varying degrees of disruption, vesiculation of the cristae being evident after 5 hours exposures and matrical inclusions after 18 hours exposures. Similar cristal vesiculation was seen after 30 minutes treatments with potassium cyanide. Providing treatments were terminated before cell viability was lost, all mitochondrial abnormalities were reversible on return to normal culturing conditions. The longer the period of anaerobiosis the longer was the recovery time required for the return of normal mitochondrial structure and the re-equilibration of control Type I to Type II mitochondrial frequencies. The relationship between mitochondrial conformation and functional integrity is discussed in the light of these findings.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01275801
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    Ultrastructural cytochemistry of atrial muscle cells. VI. Comparative study of specific granules in right and left atrium of various animal speciesSupported in part by the Medical Research Council of Canada (Grant MT-1973), the Quebec Heart Foundation, the J:L. Levesque Foundation, the Ministry of Education of Quebec (Formation de chercheurs et action concertee) and the Fonds de I'Universite de Montreal (CAFIR). (1979)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 193 (1979), S. 55-69 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Specific (A-, B- and D-) granules as well as multivesicular bodies (MVB) with a dense core from the right and left atrium of a variety of mammals (mouse, rat, hamster, guinea pig, rabbit, young and old cat) were compared by quantitative ultrastructural and cytochemical methods as regards number, size, localization and reactivity. The number of specific granules was greater in the right atrium of the rat and lesser in the same atrium of the guinea pig. In the rat this difference was due to a greater number of all three types of specific granules in the right atrium. In the guinea pig, both A and B granules were more numerous in the left atrium. A greater number of granules was also found in the left atrium of hamster, rabbit and young and old cat but the difference with the right atrium was not significant. All atria contained the same type of specific granules but A- and B-granules were present in the left atrium not only in the paranuclear area, as in the right atrium, but also throughout the sarcoplasm. In both atria, all specific granules were argen-taphobic when stained according to the periodic acid-thiocarbohydrazide-silver proteinate technique of Thiery. They reacted positively to phosphotungstic acid-hydrochloric acid (PTA) (pH 0.3) as did the cell coat, residual bodies (Cgranules), lysosomes, Z-disks and a small portion of the Golgi complex. The PTA and PAS stains were abolished by acetylation, restored by saponification, unchanged by methylation and greatly diminished by sulfation. The MVB with a dense core, identical to those already noted in the cells of various endocrine glands, and thought to be crinophagic, were rare in the right atrium of all species and absent in that of the rat. They were much more numerous in the left atrium, particularly of hamsters, guinea pigs and cats and, to a lesser degree, of mice, rabbits and rats. They were silver negative. Their dense core reacted to PTA but their matrix, contrary to classical MVB without a dense core, did not.
    Additional Material: 4 Tab.
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    Ultrastructural features of the distended pulmonary arteries of the normal rat (1979)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 193 (1979), S. 71-97 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Detailed study has been made of the structure of the normal pulmonary artery of rat by both light (1-μ sections) and electron microscopy. After tying the pulmonary veins at the hilum, the lungs were fixed by simultaneous injection of glutaraldehyde into the pulmonary trunk and trachea. Study of distended arteries allows precise measurement and assessment of normal lung structure.Four regions of the pulmonary artery can be identified by wall structure and are described here-muscular, partially muscular, non-muscular and the newly described thick-walled oblique muscular. Electron microscopic examination has demonstrated in the non-muscular regions of the partially muscular arteries, an “intermediate” cell and in the non-muscular arteries, a pericyte. The intermediate cell lies internal to the single elastic lamina but external to the endothelial cell, is surrounded by its own basement membrane and contains filaments mainly along the adluminal region of the cell. The pericyte also lies internal to the single elastic lamina, is within the basement membrane of the adjacent endothelial cell and has previously been reported in the lung only in the walls of alveolar capillaries. The structure of the intermediate cell and its position suggest it is a transitional stage between the pericyte and smooth muscle cell.
    Additional Material: 3 Tab.
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    URL: http://dx.doi.org/10.1002/ar.1091930106
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    Ultracytochemical localization of Na+,K+-activated ATPase in chloride cells from the gills of a euryhaline teleostThis Research was Supported by Grant P4B 3634 from the National Science Foundation. The Authors thank Ms Natalia Raphael for her contributions to this study (1979)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 193 (1979), S. 99-129 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The activity of the electrolyte transport enzyme, sodium, potassium-activated adenosine triphosphatase (Na+,K+-ATPase), in the gills of the pinfish, Lagodon rhomboides, increased markedly following transfer of fish from brackish water to seawater. Cytochemical localization of Na+,K+-ATPase via its potassium-dependent phosphatase (K+-NPPase) activity in the branchial epithelium of pinfish adapted to seawater demonstrated that chloride cells are the major sites for the enzyme. Subcellularly, the heaviest depositions of reaction product were observed lining the cytoplasmic membrane surfaces of the labyrinth of anastomosing plasma membrane tubules that ramifies throughout the chloride cell cytoplasm. Enzyme activity was demonstrated also on the cytoplasmic surface of the apical crypt membrane and on the cytoplasmic surfaces of vesicles in the cytoplasm subjacent to the crypt. Deletion of potassium from the cytochemical incubation medium or inclusion of 10 mM ouabain abolished the reaction products associated with these membranes. The significance of these cytochemical results is discussed with reference to current hypotheses of chloride cell function.
    Additional Material: 3 Ill.
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    URL: http://dx.doi.org/10.1002/ar.1091930107
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    Southern Society of Anatomists. Program and abstract presented at the seventeenth annual meeting, Birmingham, Alabama, October 19-21, 1978 (1979)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 193 (1979), S. 149-177 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1091930109
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    Impregnation of soft biological specimens with thermosetting resins and elastomersPatent pending. (1979)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 194 (1979), S. 247-255 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: A new method for impregnation of biological specimens with thermosetting resins and elastomers is described. The method has the advantage that the original relief of the surface is retained.The impregnation is carried out by utilizing the difference between the high vapor tension of the intermedium (e.g., methylene chloride) and the low vapor tension of the solution to be polymerized. After impregnation, the specimen is subject to polymerization conditions without surrounding embedding material.The optical and mechanical properties can be selected by proper choice from various kinds of resins and different procedures, for example, by complete or incomplete impregnation. Acrylic resins, polyester resins, epoxy resins, poly-urethanes and silicone rubber have been found suitable for the method.Excellent results have been obtained using transparent silicone rubber since after treatment the specimens are still flexible and resilient, and have retained their natural appearance.
    Additional Material: 5 Ill.
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    URL: http://dx.doi.org/10.1002/ar.1091940206
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    Ultrastructure of the fetal rat anterior pituitary gland at term and during prolonged gestationSupported by the Medical Research Council of Canada, Grant MA4740. (1979)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 194 (1979), S. 257-265 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The ultrastructure of the fetal rat anterior pituitary gland is described at term (day 22) and during experimentally prolonged gestation (days 23, 24, 25). The latter was achieved by daily subcutaneous injections of five mg progesterone to gravid females from the twentieth through the twenty-fourth day. Using morphological criteria for classifying pituitary cells (Moriarty, '73), six different types were observed: thyrotrophs, gonadotrophs, corticotrophs, mammotrophs, somatotrophs and non-granulated cells.During prolonged gestation (days 24 and 25 only), cells designated as cor-ticotrophs revealed changes suggesting increased activity, i.e., proliferation and dilation of the endoplasmic reticulum, dilated Golgi membranes and a reduction of secretory granules. In addition, meconium staining, which is indicative of fetal distress, was also observed on days 24 and 25. The ultrastructural changes noted suggest an increase in corticotroph activity due to fetal hypoglycemia and hypoxia which are known to occur as a result of placental insufficiency during prolonged gestation (Roux et al., '64; Vorherr, '75; Thliveris, '76).
    Additional Material: 3 Ill.
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    URL: http://dx.doi.org/10.1002/ar.1091940207
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    Histochemical study of ovarian hydroxysteroid dehydrogenase activity during normal pseudopregnancy in the rat (1979)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 194 (1979), S. 273-281 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: 3β-Hydroxysteroid dehydrogenase activity was localized in ovaries removed from rats during normal pseudopregnancy. Ovarian sections were incubated in media containing either pregnenolone (P-one) or dehydroepiandrosterone (DHA). P-one-3β-HSD activity appeared strongest on day 6 whereas DHA-3β-HSD was highest on day 13. NADH2 diaphorase activity was localized and observed to be greater than 3β-HSD.Both P-one- and DHA-3β-HSD activities were localized in primary-, secondary- and tertiary follicles; granulosa and theca interna cells have a variable potential for steroidogenesis. Mature tertiary follicles appear most responsive of the follicular population in steroidogenic potential. In atretic follicles, theca interna cells showed a marked ability for potential steroid production and may be one of the more stable sites of steroidogenesis. Interstitial tissue is a major site for potential estrogenic and progestational biosynthesis during the functional life of the ovary. Luteal cells exhibited strong ability for DHA-3β-HSD activity during the ongoing process of pseudopregnancy while P-one-3β-HSD activity declined in these cells as pseudopregnancy progressed. In summary, at the onset of pseudopregnancy, it appears that ovarian P-one-3β-HSD activity is greater than DHA-3β-HSD activity. Near the termination of this condition, DHA-3β-HSD activity is greater than P-one-3β-HSD activity in the rat.
    Additional Material: 9 Ill.
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    URL: http://dx.doi.org/10.1002/ar.1091940209
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    Testosterone and UVL-B stimulation of epidermal melanocytes in rat scrotal skinSupported by USPHS Grants AM-20669-16 and DE-01766 from the National Institute of Arthritis, Metabolism, and Digestive Diseases and the National Institute of Dental Research. (1979)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 193 (1979), S. 43-53 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The effects of UVL-B and/or testosterone replacement therapy are compared in normal and castrated rats in order to determine whether testosterone is required for UVL-B (280-315 nm) stimulation of melanogenesis in the testosterone-dependent epidermal melanocyte system of the scrotal skin of black Long Evans rats. Testosterone is not a prerequisite for UVL-B stimulation of melanocytes as in both castrates and normal animals the melanocytes respond to UVL-B by increases in size, length and number of dendrites (dendriticness), and tyrosinase activity (intensity of Dopa reaction). Addition of testosterone to castrates does enhance the effects of UVL-B. However, UVL-B with or without testosterone cannot maintain normal melanogenesis in rats irradiated immediately after castration nor can it restore normal melanogenesis following long term castration. Both the amount of UVL energy/exposure and the number of exposures are important variables in stimulation of the epidermal melanocytes. Administration of a dose of UVL-B to castrates in a single exposure is ineffective, while the same overall dose spread over several exposures increases the size and dendriticness of melanocytes. Testosterone and UVL-B act synergistically in affecting melanogenesis although neither singly nor in combination are they able to fully restore normal melanogenesis.
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    Masthead (1979)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 194 (1979) 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1091940201
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    A fine structural analysis of the myelin sheath in rat spinal roots (1979)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 194 (1979), S. 201-211 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The present study is concerned with the fine structure of the myelin sheath in rat and monkey spinal roots. Pictures are obtained that show that the intraperiod gap of the myelin sheath is continuous and opens into both the internal and external mesaxons, which in turn open into the periaxonal and endoneurial extracellular spaces. These results are compatible with the idea that the intraperiod gap is a cleft that traverses the thickness of the myelin sheath and links the periaxonal and endoneurial extracellular spaces.
    Additional Material: 6 Ill.
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    URL: http://dx.doi.org/10.1002/ar.1091940203
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    Further observations on tubulobulbar complexes formed by late spermatids and sertoli cells in the rat testis (1979)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 194 (1979), S. 213-232 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The present study extends earlier findings (Russell and Cler-mont, '76) by describing additional details of the structure, relationships, distribution, origin, and fate of tubulobulbar complexes. Freeze-fracture and thin section observations reveal few membrane associated particles and no appreciable cell coat in the membranes forming the tubulobulbar complex. The opposing plasma membranes of the complex do not appear to form a junction, although junctional formation might be expected by the close proximity (4.0 nm apart) of the Sertoli and spermatid plasma membranes. Sertoli filaments of the network which encircle the tubular portion of the complex measure 5.0-7.0 nm across and appear to insert into the Sertoli plasma membrane. Tubulobulbar complexes initially form in association with a cell surface modification (bristle-coated pit) of the Sertoli plasma membrane. The first tubulobulbar complexes develop large bulbous components (up to 2.5μm across), which soon lose connection with the spermatid and become incorporated into large phagocytic vacuoles (secondary lysosomes). As bulbs undergo dissolution, newly formed tubulobulbar complexes are observed to replace these structures. Thus, the data indicate that more than one generation of tubulobulbar complexes develop. Moreover, the tubular and bulbous portions of most dissociating complexes (as well as neighboring Sertoli lysosomes) show acid phosphatase activity. Near the time of sperm release, all complexes at the concave aspect of the spermatid head are resorbed. New tubulobulbar complexes, many lacking terminal bulbous dilations, form at the dorsal and lateral aspects of the spermatid head. These persist even after all ectoplasmic specializations and most Sertoli cytoplasm have been withdrawn from a position facing the spermatid head. The presence of tubulobulbar complexes just prior to the time of sperm release is in support of previous findings indicating that tubulobulbar complexes participate in anchoring the head of the late spermatid prior to sperm release. Past the zone of sperm release a few abnormally shaped cells, which had not been released with other spermatids of the same generation, display intact tubulo-bulbar complexes. The persistence of these and other structures may be the means by which abnormally shaped spermatids are retained and prevented from traversing the male duct system.
    Additional Material: 34 Ill.
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    URL: http://dx.doi.org/10.1002/ar.1091940204
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    Intramitochondrial rod-like inclusions in human breast tumors (1979)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 194 (1979), S. 267-272 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Ultrastructural screening of human breast tumor biopsies has revealed, in 43% of the carcinomas, and in 16% of the benign lesions examined, the presence of rod-like inclusions in mitochondria of epithelial cells. The inclusions are cylindrical in cross section, with a diameter of about 50 nm, and show regular striations in longitudinal sections. They are probably derived from cristae. Mitochondria with inclusions are usually enlarged and are found most frequently in epithelial cells of invasive tumors and near zones of tumor necrosis. Similar although not identical inclusions have been observed by other investigators in the liver of women with hydatidiform mole or choriocarcinoma, of pregnant women, and of women taking oral contraceptives. Identical inclusions have been found in mitochondria of HeLa cells treated with anti-HeLa serum. Although no clear-cut correlations were found between the finding of inclusions and parameters such as age of patients, nuclear grade or lymphocytic infiltration of the breast tumors, it is conceivable that the presence of mitochondrial inclusions reflects the effect of hormonal or of immunological factors.
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    The distribution of alkaline phosphatase activity in normal and cross-species regenerated rat and mouse taste buds (1979)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 194 (1979), S. 283-291 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Alkaline phosphatase (ALK Pase) activity can be detected histochemically in the taste buds of rats but not mice. Since taste buds develop, regenerate and are maintained under the influence(s) of the sensory nerve it was decided to study cross-species regenerated buds of these two animals to determine whether the nerve also regulated ALK Pase development in taste cells. Grafts of rat sensory ganglion and mouse tongue or mouse ganglion and rat tongue were combined in the anterior chamber of the eyes of immunologically-deficient nude mice and the cross-species buds that developed at 35 days were examined histochemically for ALK Pase. The results revealed that the rat nerve did not cause ALK Pase to appear in any buds found in mouse tongue grafts and that mouse nerve could support buds containing ALK Pase in rat tongue tissue. Because the cross-species regenerated buds were histochemically characteristic of those normally found in rat or mouse tongue, there is no evidence that the foreign nerve altered gene expression for ALK Pase in the target organ, and the action of the nerve on gustatory epithelium appears to be that of activation and maintenance.
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    Masthead (1979)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 194 (1979) 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1091940301
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    Replacement of fatty marrow by active granulocytopoietic bone marrow following transplantation of mammary carcinoma into mice (1979)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 195 (1979), S. 31-45 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The development of granulocytic hemopoiesis in the fatty marrow of metatarsal bones and caudal vertebrae of adult mice was studied in histological sections for up to six weeks following subcutaneous innoculation of granulocytosis inducing mammary carcinoma. The initial events observed were increase and engorgement of fatty marrow sinusoidal vascular beds, accompanied by numerous lymphoid mononuclear cells in the sinusoids and in the stroma. Foci of granulocytopoiesis appeared admixed with lymphoid cells in the stroma and near the endosteum. Hyperplastic granulocytopoiesis eventually predominated in the marrow of these bones as well as the femoral and sternal marrow of tumor bearing mice. The morphological findings suggested the possibility of stem cell and progenitor cell migration into fatty marrow, but activation of dormant stem cells could not be ruled out. The prevalence of granulocytopoiesis in the entire skeletal marrow in tumor bearing mice may seriously impair the production of other cellular components of the marrow including lymphocytes, reduced production of the latter would imply serious compromise for the immune system of the tumor bearing animals.
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    American association of anatomists (1979)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 195 (1979), S. 115-242 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1091950110
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    The occurrence of ciliated and mucous cells in the peripapillary trench of the rat tongue (1979)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 195 (1979), S. 301-309 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Ciliated cells surrounded by mucous cells were found near the base of the peripapillary trench of the rat circumvallate papilla. The ciliated cells occurred singly or in small groups. Each cilium showed the nine plus two filament pattern characteristic of motile cilia.We consider that these cells function in wetting the taste pores, circulating saliva containingtastants and removal of debris from the papillary trench.
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    URL: http://dx.doi.org/10.1002/ar.1091950205
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    Selective labeling of mesenteric lymph nodes: Cell production and emigration ofnewly formed lymphocytes to other organsPartly presented asaposter at the VI. Intern. Conf. on Lymphatic Tissues and Germinal Centers, Damp, Germany, June 11-16, 1978. Supported by the Deutsche Forschungagemeinachaft. Pa 240/1. (1979)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 195 (1979), S. 341-355 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Lymphocyte production by mesenteric lymph nodes of normal young pigs was studied by intranodal injections of either tritiated thymidine or tritiated deoxycytidine as DNA precursors. One or two days after selective labeling of the mesenteric lymph nodes the relative and absolute number of lymphocytes derived from mesenteric lymph nodes were determined autoradiographically in the following organs: mesenteric, cervical and inguinal lymph nodes, spleen, thymus, bone marrow, peyer'spatches, tonsil, different regions of the gut, lung and liver. The overall cell production of mesenteric lymph nodes, as derived from the sum of all labeled cells one day after labeling, was estimated to be about 7 × 109 lymphocytes. Up to 40% of all newly formed lymphocytes had already left the lymph nodes within one day and were found in all organs studied. There was a preferential homing to the mucosa of the small intestine, but a considerable number migratedto the spleen and even to the thymus and bone marrow. In lymphoid organs all labeled cells were small and medium-sized lymphocytes one and two days after labeling. In cervical lymph nodes, spleen, tonsil and peyer's patches the relative distribution to T and B cell areas was determined. There was an obvious preference of newly formed lymph node cells to home to T cell areas. The differences of labeling between thymidine or deoxycytidine were surprisingly low.
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    Fine structural studies on white adipocyte differentiationThis work was supported by NIH Grants HL 18283 and GRS 5 S01 RR05356. (1979)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 195 (1979), S. 63-72 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The differentiation of mammalian white adipocytes from prenatal through early postnatal periods was studied by light and electron microscopy in C57BL mice. Anatomical regions chosen for this study were the epididymal, mesometrial, mesenteric and inguinal fat pads. In each of these regions, adipocytes differentiated from fibroblast-like cells (preadipocytes) characterized by an ovoid nucleus, profiles of rough endoplasmic reticulum, microtubules, microfilaments, spherical mitochondria, and small multiple lipid inclusions. Preadipocytes of the inguinal fat pad were first observed prior to birth (17-19 days), whereas, in the other anatomical sites, these cells were not observed until one to three days postnatally. As differentiation proceeded, and as the adipocytes assumed a spherical shape, there was a progressive decrease in the amount of rough endoplasmic reticulum and microfilaments concomitant with transient glycogen storage and an increase in the size of lipid droplets. Mature unilocular adipocytes were observed in the inguinal fat pads at three days of age. On the other hand, these cells did not appear until seven days after birth in the epididymal fat pad, mesometrium and mesentery. Regardless of the anatomical region studied, the differentiation of preadipocytes to adipocytes proceeded similarly. Preadipocytes could not be distinguished from fibroblasts morphologically within the fat depots studied. Adipocytes at the mid-stages of differentiation and in all regions studied occasionally exhibited close intercellular contacts of varying morphology.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1091950106
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    Ultrastructural localization of histones in epiphyseal chondrocytes (1979)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 195 (1979), S. 109-113 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Epiphyseal growth plate chondrocyte nuclei of growing rats were studied for histone content using the ammoniacal silver technique. This stain, which is specific for the demonstration of the arginine-rich fractions of histones, revealed that growth plate nuclei vary in their histone content. Nuclei of cells of the proliferating zone revealed a significantly greater amount of postformalin ammoniacal silver deposit consistent with the presence of arginine-rich histones.
    Additional Material: 4 Ill.
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    URL: http://dx.doi.org/10.1002/ar.1091950109
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    Cell junctions in the endothelia and connective tissue of the rat choroid plexus (1979)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 195 (1979), S. 73-93 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Cell junctions between endothelial cells and between connective tissue cells (leptomeninges) of the rat choroid plexus were studied by freeze-fracture. Special attention was paid to the problems of identifying fractured cell membranes and fracture faces. Tight junctions in arterioles are comprised of continuous small-meshed networks of strands appearing as shallow grooves with aligned particles on E-faces, and low ridges with some particles on P-faces. In the fenestrated capillaries the tight junctions are represented by discontinuous, isolated slightly elevated ridges with some particles on P-faces, and corresponding shallow grooves devoid of particles on E-faces. Junctions in venules appeared as a less developed version of the junctions in the fenestrated capillaries. Extended areas of the meningeal cell membranes exhibit cell junctions. Tight junctions in this tissue are comprised of a loose discontinuous arrangement of isolated, often branched junctional elements. On P-faces the elements appear as elongated crests with a distinct furrow running along the edge of the crest, occupied by a few particles. On E-faces corresponding elongated impressions are present and occupied by aligned particles and a few short bars. The arrangement of these tight junction elements are not supportive of any significant barrier function. Small gap junctions occur “freely” in the membrane, or they are sometimes surrounded by tight junction elements. Very large gap junctions (up to about 10,000 particles) are mostly surrounded and interconnected by tight junctional elements. The possible significance of the variability of gap junction size, and the relation to tight junction elements is discussed.
    Additional Material: 1 Ill.
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    URL: http://dx.doi.org/10.1002/ar.1091950107
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    Resorption of connective tissue in the gingiva of the mouse incisor (1979)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 195 (1979), S. 95-107 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: During eruption of the mouse incisor part of the periodontal ligament moves along with the tooth in occlusal direction and is degraded in the gingiva, just apically of the junctional epithelium. When studied with the electron microscope some connective tissue cells (fibroblasts) showed disorganization of both nucleus and cytoplasm. In other cells vacuoles were observed containing partly degraded cellular constituents. Sometimes within these vacuoles electron dense material with the appearance of clumped chromatin was observed. On the basis of this observation it is concluded that heterophagocytosis contributes to the process of fibroblast breakdown. The ultrastructure of these heterophagic cells resembled that of fibroblasts.Collagenous fibrils were observed within cytoplasmic vacuoles of fibroblasts indicating that collagen is phagocytosed and probably digested in the lysosomal apparatus. Part of the intercellularspace was filled with a homogeneous material of moderate electron density, intermingled with some collagenous fibrils.Within some cells of the junctional epithelium partly degraded material was observed which may indicate that the epithelium contributes to the removal of residual products of connective tissue degradation.
    Additional Material: 1 Ill.
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    URL: http://dx.doi.org/10.1002/ar.1091950108
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  • 78
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    Surface area ratios II. A stereological method for estimating changes in average cell volume and frequency (1979)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 195 (1979), S. 257-264 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The study describes a stereological method for detecting relative changes in cells-with respect to their average volumes and to their frequencies within one cm3 of exocrine cells. The method is based on surface area ratios (Bolender, '1979) and can detect changes comparable to those obtained with the numerical density approaches (Loud, '68; Weibel et al., '69; Bolender, '74; Williams, '77). The new method requires only intersection counts- the same ones that are used for surface density estimates- and, as a result, avoids the difficult and often problematic procedures of the numerical densities. In analyzing the cellular changes in vitro, the choice of either a zero or timed control was found to exert a major influence on the results. Timed controls were required to “isolate” the experimentally induced changes from those Produced by the incubation medium.
    Additional Material: 2 Ill.
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    URL: http://dx.doi.org/10.1002/ar.1091950202
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    “Silver dust” - a tool to study growth interrelationships between bone, periosteum and muscle (1979)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 194 (1979), S. 539-545 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: A cylinder of gelatin containing silver spongy granules was placed in and lay within the masseter muscle, the periosteum, and the mandible, and terminated in the medial pterygoid muscle of young (3 month-old) growing miniature pigs.On the basis of an animal sacrificed one week after placement of the cylinder, it was found that the suspending gelatin was removed by cellular activity. Nine months later the remaining animals were sacrificed. Periodic X-rays were taken during the course of the experiment. After sacrifice, the mandible and associated tissues were histologically examined. The results of this study suggest that the silver granules in the muscles maintained their location during growth; the silver granules in the mandible moved forward with mandibular growth. “Slippage” appeared to occur external to the fibrous layer of the periosteum; the site of movement was revealed by the trail of the silver granules. The described method should prove of value in studying the growth interrelationships between bone, periosteum, and muscle.
    Additional Material: 3 Ill.
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    Chromatin substructure: An electron microscopic study of thin-sectioned chromatin subjected to sequential protein extraction and water swelling procedures (1979)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 194 (1979), S. 547-562 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Electron microscopic observations and measurements were made on thin-sectioned chromatin fibers and fibrils obtained from nuclei of mature chicken erythrocytes. The nuclei were isolated in low ionic strength gum arabic and octanol then extracted sequentially with (1) 0.14M NaCl, (2) 0.25 N HCl, (3) buffer saturated phenol, (4) hot 5% SDS and 0.14 M 2-mercapto-ethanol and, (5) 0.4 N NaOH. The amount of nuclear protein removed at each of the first four extraction steps was 1, 86, 3 and 11% of the total, respectively. Each extract was characterized by electrophoretic profiles. At each extraction the chromatin was fixed by adding large quantities of a mixture of equal volumes of sodium cacodylate buffered 8% (w/v) glutaraldehyde (pH 6.8) and 2% OsO4 (w/v), directly into (1) an aliquot of the chromatin in extraction fluid, and (2) an aliquot of the chromatin after water washing and swelling. Three size classes of chromatin structure were seen in thin sections prepared for high resolution transmission electron microscopy and stained with uranyl acetate and lead citrate. A thick fiber of about 25 + nm diameter was the predominant large fiber seen in freshly isolated nuclei or in nuclei after salt extraction. This 25 + nm fiber has a substructure consisting of 3.2-5.2 nm diameter fibrils. After water swelling of such freshly isolated or salt extracted nuclei a fiber of about 10 nm diameter was the predominant large fiber instead of the 25 nm diameter fiber. The HCl extraction step which is known to remove histones, caused the disappearance of both the 25 nm and the 10 nm fibers. High magnification (600,000 × ) micrographs of the chromatin at all procedural steps, except the last NaOH step, reveal the fibril to be omnipresent. This fibril tends to decrease somewhat in diameter during the protein extraction steps to a 2.5 nm diameter fibril after the hot SDS extraction. A fibril of 2.5 nm diameter is expected of naked double helical DNA stained with a positive stain. The NaOH, which is known to denature DNA, completely destroyed the remaining fibril. We interpret our results to indicate that the larger chromatin fiber seen in micrographs of thin-sectioned chromatin has a fibrillar substructure which probably represents a double coil of native DNA which may have a thin protein coating of its own. The latter fibril may in turn be wrapped around a hydrophobic histone domain, perhaps reflected in the 10 nm diameter fiber which is seen upon swelling of the chromatin. This 10 nm diameter fiber is thought to be further packaged by folding into the 25 + nm diameter chromatin fiber most frequently reported in thin sections of eukaryotic cell nuclei in situ.
    Additional Material: 24 Ill.
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    URL: http://dx.doi.org/10.1002/ar.1091940408
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    Phenytoin (dilantin)-induced cleft lip and palate in a/j mice: A scanning and transmission electron microscopic studyThese studies were Supported by the following grants: DE 02668, DE 05113 and RR 05333. (1979)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 195 (1979), S. 243-255 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: High incidences of cleft lip and palate (CLP) Produced by maternal intraperitoneal administration of 75 mg/kg pehnytoin on gestational day 10 to A/J mice are associated with asevere size reduction in the lateral nasal process. Scanning and transmission EM analyses of this region demonstrate a marked change in the morphology of the mesenchymal cells underlying the surface epithelium in treated versus control day 11 (7-tail somite) embryos: long, branching cellular processes, which form a dense meshwork that appears to interact with the epithelial basement lamina in control embryos are undeveloped or absent in phenytoin-treated embryos. The ultrastructural morphology of these cell processes is described and their possible importance in normal development is discussed. Scanning EM observations of incomplete clefts of the lip which frequently result from phenytoin treatment indicate that Simonart's bands form from fusionof the lateral and medial nasal processes in association with a lack of fusion of the maxillary process with the medial nasal process.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1091950201
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    Corpus luteum growth and plasma progesterone levels in unilaterally ovariectomized pregnant rats (1979)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 195 (1979), S. 311-316 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Unilateral ovariectomy (ULO) was carried out on rats at Day 8 of gestation: term is Day 23. The effect on peripheral plasma progesterone levels at Day 16 and on growth and cellular changes in the remaining corpora lutea (CL) at Day 17 was determined by comparison with results from control and sham-operated rats. ULO increased the volume of remaining CL by 18% at Day 17. At Day 16 this effect was only apparent in rats with three or fewer CL remaining. CL hypertrophy was due to an increase in the volume of individual luteal cells and not to cell hyperplasia. Luteal cell nuclear volume also increased as did the number of endothelial cells per corpus luteum. The proportion of the corpus luteum occupied by vascular space did not alter.ULO had little effect on mean plasma progesterone levels at Day 16 (92 ± 7 ng/mltreated; 86 ± 11 ng/ml controls). In rats with three or fewer CL remaining, however, progeserone levels were low (51 ± 7 ng/ml).It was concluded that ULO can enhance the growth of corpora lutea and perhaps increase their rate of progesterone production.
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    URL: http://dx.doi.org/10.1002/ar.1091950206
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    Cytological events associated with in vitro aged and fertilized rabbit eggs (1979)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 195 (1979), S. 357-374 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The morphogenetic events associated with rabbit eggs aged in vitro for 12 to 50 hours prior to mixing with sperm have been examined by light and electron microscopy. After 12 hours in culture, morphological alterations of the meiotic spindle and the cortex of unfertilized eggs were evident. By 24 to 50 hours in culture, unfertilized eggs contained subnuclei, structures which formed when individual and/or groups of meiotic chromosomes dispersed and became invested by a double-laminated structure reminiscent of a nuclear envelope.Although most eggs obtained 11.5 to 12 hours after induced ovulation and in vitro fertilized displayed morphogenetic patterns similar to those described for in vivo fertilized ova, some (10%) contained three pronuclei. Many eggs obtained 13 to 15 hours after induced ovulation and subsequently mixed with sperm in vitro appeared to undergo processes of fertilization typical of in vivo fertilized eggs, however, approximately 30% contained subnuclei in association with the male pronucleus. Few eggs (15%) aged 12 hours prior to in vitro fertilization displayed patterns of pronuclear development and association typical of fertilized unaged ova. Subnuclei developed in many of the fertilized ova. Supernumerary sperm nuclei, which did not develop into male pronuclei, were observed in some zygotes. Cleavage of eggs aged 12 hours prior to fertilization was abnormal or retarded. After 24 hours in culture approximately 16% of the eggs fertilized. Seventy percent of the fertilized eggs failed to Support the development of a male or female pronucleus.
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    URL: http://dx.doi.org/10.1002/ar.1091950209
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    Effect of uncouplers of oxidative phosphorylation on microtubule location and surface structure in murine mast cells (1979)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 195 (1979), S. 375-386 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: “Uncouplers” of oxidative phosphorylation (sodium azide, DNP, and oligomycin) alter the location of microtubules within murine mast cells. Both cytoplasmic microtubuless, perpendicular to the plasma membrane within cell surface folds, and intranuclear microtubules wereobserved. In addition, one or more dense plaque-like structures adjacent the plasma membrane in mast cells appeared following incubation in the antimetabolities. Intranuclear microtubules and cytoplasmic microtubules within the cell surface ridges disappeared in azide-treated mast cells that were reincubated or “recovered” in PBS. However, both these structures remained in oligomycin- and DNP-treated murine mast cells following reincubation.
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    URL: http://dx.doi.org/10.1002/ar.1091950210
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    Milk lipid absorption and chylomicron production in the suckling rat (1979)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 195 (1979), S. 397-412 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Structural correlates of milk lipid absorption and chylomicron production were studied in 10-day-old suckled rats. The gastric and duodenal contents and duodenal mucosae were examined with the light and electron microscopes. In the gastric lumen the milk lipid globule cores were smooth, circular and uniformly electron opaque. Many membranes and lamellar structures with a trilaminar and multilamellar appearance were adherent to the peripheries of the cores. In the central duodenal lumen the milk lipid globule cores were also smooth, circular and uniformly electron opaque. Very few milk lipid globules in the duodenal lumen showed adherent membranes or lamellae. Membrane fragments and lamellae were present in the lumen separate from the milk lipid globules. In the duodenal lumen between villi the milk lipid globules had multiple electron lucent indentations of the core. It is believed that the irregular peripheries of the milk lipid globule cores are the result of lipolysis within the duodenal lumen acting at the milk lipid globule surface. This lipolysis of triacylglycerol would produce amphiphilic lipids which may result in the electron lucent spaces at the milk lipid globule periphery. The absorptive epithelial cells along the length of the duodenal villus varied in structure relative to their position at the tip, middle, or base of the villus. Typical mid-villus epithelial cells contained lipid droplets averaging 0.3-μm diameter in the smooth and rough endoplasmic reticulum and in Golgi complexes in the apical cytoplasm. Villus tip and villus base cells contained large lipid droplets between 7-16 μm. Only a few 0.3-μm lipid droplets were present within these cells. These large lipid droplets appeared to be accumulations of triacylglycerol present in the apical cytoplasm associated with lamellar and membranous structures. Numerous chylomicrons were present between epithelial cells located in the middle region of the villus while significantly fewer chylomicrons were seen between epithelial cells at the tip and base of the villus. These observations suggest that the cells at the middle of the duodenal villus of suckling rats were more efficient in the production of chylomicron triacylglycerol derived from incoming milk triacylglycerol than cells at the tip and base of the villus.
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    URL: http://dx.doi.org/10.1002/ar.1091950301
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    The cellular pattern of corpus luteal growth during pregnancy in the rat (1979)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 193 (1979), S. 823-830 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The cellular pattern of corpus luteal (CL) growth was studied in rats at Days 6, 10, 12, 14, 16, 17 and 22 of pregnancy: term is Day 23. Measurements were taken of the percentage of the CL occupied by luteal cells, connective tissue and vascular space, luteal cell and nuclear volumes, and the number of luteal and endothelial cells in each of three CL in both ovaries of five rats at each stage of pregnancy. Total CL volume increased from 1.08-3.23 μl over Days 10-17. This was mainly due to an increase in luteal cell volume from 3.72 pl to 9.30 pl. Neither the number of luteal cells per CL (range 212,000-287,000) nor the percentage of the CL occupied by luteal cells (range 85-90%) had much influence on growth. Nuclear volume increased roughly in proportion to cytoplasmic volume but near term it decreased despite little change in cytoplasmic volume. The number of endothelial cells per CL increased steadily from 398,000 at Day 6 to 1,545,000 at Day 22. There was a strong negative correlation (r = -0.78) between the number of luteal cells per CL and mean luteal cell volume that was evident at all stages of pregnancy. There was a positive, but weaker correlation (r = 0.35) between number of luteal cells and CL volume. Thus, CL volume seems to be partly determined by the number of luteal cells at Day 6 but this effect is moderated by local control of luteal cell volume.
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    Distribution of glycogen in prefusion human palatal epitheliumThis work supported by NIDR Grant R01 DE 04695-02. (1979)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 193 (1979), S. 831-855 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Various stages of embryonic human secondary palatal development were examined for the presence of epithelial glycogen. Utilizing periodic acid-Schiff's reagent staining of thich plastic sections and osmium ferrocyanide enhancement of thin sections, dramatic changes in epithelial glycogen distribution were noted during palatogenesis. Prior to fusion, the epithelium destined to adhere in the midline exhibited a marked diminution of glycogen in the superficial cell layer. This cell layer was composed of slender dense cells and cuboidal cells undergoing lysis. Adjacent nonfusing epithelium was markedly different and contained large glycogen reserves in its superficial cell layer. Glycogen may play a role either as precursor for specific adhesive macromolecules or as a physical agent capable under the influence of appropriate enzymes of causing cell lysis.
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    Scanning electron microscopy of frozen sections: A method for studying selected planes in small specimens (1979)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 193 (1979), S. 857-861 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: A procedure for studying small biological specimens with the SEM, using frozen sections of fixed material is described. Controlled fixation schedules and convenient handling of sections insure optimal preservation of cell shape and size. The greatest advantage of the method presented is to allow scanning through any desired plane of the specimen, even in those planes parallel to an epithelial face. SEM of frozen sections is thus helpful for the study of cell distribution and organization within a tissue.
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    Degeneration of mouse oocytes in response to polycyclic aromatic hydrocarbons (1979)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 193 (1979), S. 863-881 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Destruction of mouse oocytes in primordial and small primary follicles in response to treatment with 3-methylcholanthrene (MC) was studied at the ultrastructural level. Four-week old C57Bl/6N (B6) strain mice received a single injection of 80 mg/Kg MC in corn oil intraperitoneally. Controls received only corn oil. Ovaries from animals were prepared for light and electron microscopic examination at specified intervals after treatment. The number of primordial follicles remained constant in control animals. In contrast, their number decreased significantly (P 〈 0.01) by three days, and they were depleted by seven days after MC treatment. Subtle degenerative modifications were noted in the ooplasm of primordial follicles two days after treatment. These changes consisted of vesiculation of mitochondrial cristae, increased electron density of the mitochondrial matrix, myelin structures in lipid droplets and in mitochondria. More advanced stages of degeneration of primordial follicles were characterized by further vesiculation or disappearance of mitochondrial cristae, chromatin clumping, and increased density of the ooplasm. Small primary follicles had undergone similar initial degeneration as primordial follicles. In more advanced stages of degeneration nuclear and cytoplasmic contents condensed, endoplasmic reticulum, Golgi complex and mitochondria swelled, small vesicles and multivesicular bodies appeared. In the most advanced stages of degeneration of small primary follicles it appeared that small portions of the oocyte were engulfed by the surrounding follicular cells. It is concluded that exposure of B6 mice to a single dose of MC results in atresia of oocytes in primordial and small primary follicles. Ultrastructurally, these degenerating oocytes of treated mice looked much like the spontaneously atretic oocytes in untreated animals.
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    URL: http://dx.doi.org/10.1002/ar.1091930409
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    Distribution of wheat germ agglutinin binding sites on normal human and guinea pig bone marrow cells: An ultrastructural histochemical studySupported in part by Grant 1 R01 AM21512-01 from the National Institutes Of Health, Bethesda, Maryland. (1979)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 195 (1979), S. 641-657 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The wheat germ agglutinin-gold labeled ovomucoid (WGA-Ovo-G) method has been employed in the ultrastructural localization of WGA surface receptor sites on gluteraldehyde-fixed normal human and guinea pig bone marrow cells. The number of gold particles per micron of cell surface were counted and data subjected to statistical analysis. WGA labeling ofthe bone marrow cells was found to be quite heavy and evenly distributed along the cell surface. Only macrophages, in both species, and a number of lymphocytes in the guinea pig exhibited limited WGA labeling. Comparison of morphologically similar cell types in human and guinea pig marrow indicated that WGA receptor sites were more abundant on the guinea pig hemic cells. WGA surface labeling was found to be not only related to cell type and to animal species but, in some instances, varied with the stage of maturation of a given cell line; viz., neutrophilic cell series in both man and guinea pig and erythrocytic cell series in the guinea pig.
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    Morphology of the atrioventricular node, bundle and proximal bundle branches: A study employing computerized reconstructionThis work was supported by a grant from the Greater Los Angeles Affiliate of the American Heart Association. (1979)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 195 (1979), S. 699-706 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The morphology of the human atrioventricular node, atrioventricular bundle and bundle branches is described. A block of tissue bounded by the ostium of the coronary sinus, the pars membranacea, the septal leaflet of the tricuspid valve and the atrial and ventricular septa is removed. This block is then sectioned serially from the right endocardial surface in the frontal plane of the heart. Sectioning in this way produces fewer sections than from techniques previously described. Outlines of the atrioventricular node, atrioventricular bundle and proximal bundle branches are digitally registered and stored in a computer. Three dimensional reconstructions of the structures are then generated by computer and displayed on an oscilloscope so that the entire three dimensional image can be rotated in any plane. Stereoscopic image pairs are produced to assist perception of the shape of the atrioventricular node, bundle and branching patterns of the bundles. This technique is unique in that it describes a method from which a relatively small number of histologic sectionsare generated permitting not only a complete histologic examination, but also a study of the morphology of the area.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1091950409
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    Morphogenesis of rod cells in the retina of the albino rat: A scanning electronmicroscopic studySupported by United States Public Health Service Grant EY 01511 from the National Eye Institute. (1979)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 195 (1979), S. 707-717 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: This study presents scanning electron microscopic (SEM) observations of topographicalchanges that occur during morphogenesis of rod cells in the albino rat. Correlative transmission electron microscopy (TEM) was also utilized. Albino rats ranging in age from birth to three weeks were used for the study. Tissues were prepared by conventional methods for SEM and TEM.At birth, numerous irregularly arranged inner segments extend from the external surface of the sensory retina. They are spherical, smooth surfaced and possess a randomly oriented cilium. The internal morphology of these immature inner segments is comparable to that observed in other vertebrate species. Statistical analysis reveals a rapid increase in the number of rod cells during the first week. This period is characterized by the elongation of inner segments and their associated cilia. Microvilli project from the apices of Müller cells, but not from adjacent inner segments.By day 5, cilia occasionally display small bulbous outer segments. They are more numerous by day 8 and are usually eccentrically positioned at the tips of cilia. By day 11, outer segments are abundant and frequently obscure from view the underlying inner segments and associated cilia. Elongated cylindrical outer segments are present within the posterior retina at the end of the second week. However, rod cell morphogenesis lags in the peripheral retina. Topographical variations between developing photoreceptor cells in mammalian and non-mammalian retinas are discussed.
    Additional Material: 1 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1091950410
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    Histological and ultrastructural observations of tail bud formation in the chick embryoThis paper is dedicated to the memory of the late Dr. Robert M. Sweeoey, my friend, teacher, advisor, and colleague, who introduced me to the excitement of embryogenesis. (1979)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 193 (1979), S. 131-147 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Tail bud formation was studied in chick embryos by light and electron microscopy. The caudal part of the neural groove at stage 11 is flanked by widely separated neural folds and merges posteriorly with the shallow primitive groove. The neural groove and primitive streak partially overlap. The depth of the neural groove gradually decreases antero-posteriorly within this overlap zone while the dorso-ventral thickness of the streak progressively increases. The anterior end of the streak begins to form a spherical accumulation of mesenchymal cells, the incipient tail bud, concomitant with closure of the posterior neuropore. Formation of the posterior body fold results in consolidation of the remainder of the streak into the definitive tail bud. The overlap zone between neural groove and primitive streak is retained as the tail bud forms. Thus the posterior end of the neural tube and anterior end of the tail bud overlap. The latter undergoes cavitation to form the ventral part of the spinal cord within this overlap region.The tail bud is initially continuous with an overlying, flattened layer of ectoderm and an underlying, columnar layer of endoderm. A bilaminar ectodermal epithelium forms directly above the developing neural tube as the dorsal portion of the tail bud undergoes cavitation. Most of the endodermal cells are displaced from the ventral surface of the tail bud by the posterior body fold and condensed into a disk-shaped region which ultimately gives rise to tail gut.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1091930108
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    Masthead (1979)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 193 (1979) 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1091930201
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    Distribution of surface coat material on nasal folds of mouse embryos as demonstrated by concanavalin A bindingSupported by NIH Grant 5732 DE07037-02 for craniofacial developmental (1979)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 193 (1979), S. 185-196 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: 3H-concanavalin A and the concanavalin A-horseradish peroxidase staining technique were used to study the distribution of surface coat material on the epithelium of the nasal folds and nasal groove of mouse embryos. In stages shortly before and during epithelial fusion concanavalan A stained or labeled material was present at apical surfaces of epithelial cells of the nasal groove and nasal folds. Silver grains, representing bound 3H-concanavalin A, were counted in defined areas of the nasal groove and presumptive fusion area in both anterior and posterior regions of the nasal folds. For both stages examined there was a significant increase in the amount of 3H-concanavalin A bound by presumptive fusion areas in posterior regions of the nasal folds as compared with anterior regions; i.e., the amount of surface coat was greater in the region just prior to the point of contact between the nasal folds. This finding is consistent with results from investigations of palatal shelf and neural fold fusion which suggest that increased synthesis of surface coat material is associated with adhesion and fusion of epithelial folds and shelves.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1091930202
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    A qualitative and quantitative study of rete ovarii development in the fetal rat: Correlation with the onset of meiosis and follicle cell appearance (1979)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 193 (1979), S. 197-211 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Development of the rete ovarii and its contribution to the cells of the ovary were examined in fetal rats. Histochemical and autoradiographic techniques were used for the observations between days 15 and 21 of gestation. The data presented indicate that the rete system contributes somatic cells to the ovary before birth.The basement membrane which surrounds the cuboidal epithelium of the mesonephric tubules immediately adjacent to the ovary becomes discontinuous on day 15 of gestation. The mesonephric epithelial cells in this region form a knot or clump of pleiomorphic cells, with no apparent tubular organization, and this clump later becomes surrounded by a basement membrane. On day 17 of gestation the newly established fetal rete ovarii is comprised of three regions; (1) the extraovarian mesonephric tubules (ER), (2) intraovarian cords of flattened epithelioid cells which surround the oogonia (IR), and (3) a knot or clump of cells connecting the ER and IR regions (CR). The entire rete system is enclosed by a continuous basement membrane as defined by Periodic Acid-Schiff Reagent techniques.Autoradiographic and quantitative analyses demonstrate that the ER tubule cells proliferate and are incorporated into the other regions of the rete system. These processes begin on day 17 and continue until at least day 21 of gestation. The role these mesonephric tubule cells may play in the regulation of meiosis and their early contribution to the presumptive granulosa cell population is discussed.
    Additional Material: 4 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1091930203
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    Muscle proteins and the changes in shape of avian oxynticopeptic cells in relation to secretion (1979)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 194 (1979), S. 293-309 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: A combined ultrastructural and biochemical study of the avian oxynticopeptic cell was performed. Scanning electron microscopy demonstrates that this cell undergoes great changes in the shape of its apical pole in relation to secretory activity. These changes are confirmed by transmission electron microscopy and by freeze-fracture images. The biochemical finding of actin- and myosin-like proteins in high-speed supernatants of homogenates of these cells as well as the ultrastructural and cytochemical localization of actin-like filaments in their apical poles suggest a possible participation of these proteins in the above-mentioned changes. Thus, the study of cytoplasmic matrix elements and of their organization may be highly relevant in the search for a correlation between structure and function in these cells.
    Additional Material: 19 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1091940211
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    Morphology of osteoclasts in resorbing fetal rat bone explants: Effects of PTH and AIF in vitro (1979)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 194 (1979), S. 311-323 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Osteoclastic bone resorption was studied using 45Ca-labeled fetal rat bones cultured in the presence of parathyroid hormone (PTH) and an anti-invasion factor (AIF) derived from bovine hyaline cartilage which is enriched in a collagenase inhibitor. The specific morphological expressions of osteoclasts cultured in PTH and AIF were observed in both light and electron microscopy and analyzed cytometrically. Stimulation of bone resorption with PTH revealed significant increases in the numbers and activity of osteoclasts, whereas bones cultured in the presence of AIF showed significant decreases in numbers of osteoclasts and altered cell features including the loss of osteoclast contact with bone surfaces. These structural modifications were evaluated with 45Ca release data derived from matched-pair explants of fetal rat bones, revealing the existence of a relationship between resorptive states of the cultured bones and morphological expressions of osteoclastic activity.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1091940302
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    The choroid plexus of the mature and aging rat: The choroidal epithelium (1979)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 194 (1979), S. 325-353 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The choroid plexus of mature and old rats has been examined by both scanning and transmission electron microscopy. It has been shown that the macrophages lying upon the ventricular surface of the choroid plexus have a close association with burr-like protrusions that extend from the apical surfaces of the choroidal epithelial cells. These protrusions have a dark cytoplasm filled with vesicles and tubules, and projecting from them are thin, shrunken microvilli. It is suggested that these protrusions are phagocytosed by the macrophages and that they are the source of some of the inclusions which become increasingly common within the cytoplasm of macrophages in older rats. The lateral surfaces of the choroidal epithelial cells have also been examined in the scanning electron microscope after exposure of the surfaces by dissection. In such preparations it is apparent that the elaborate interdigitations between adjacent cells are effected by irregular and vertically arranged folds confined to the basal portions of the lateral cell surfaces. Lastly, it has been shown that at the junction between the choroid plexus and the ependyma in the lateral ventricle, there are two modes of transition between the choroidal and ependymal epithelia. In one, typical choroidal and ependymal epithelial cells lie next to each other to produce a distinct and continuous boundary. In the other mode the boundary is also continuous, but there are modified ependymal cells present. These modified cells have short, relatively sparsely distributed microvilli and not more than one or two cilia.
    Additional Material: 22 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1091940303
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    Circulation of cerebrospinal fluid in the bullfrog, Rana catesbiana (1979)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 194 (1979), S. 389-403 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: In order to demonstrate features of the cerebrospinal fluid (CSF) path in the amphibian, 2-6 m̈l of either Evans Blue-albumin (EBA) or ferrocyanide were injected into the ventricular system of anesthetized bullfrogs. The animals were sacrificed 1 to 135 minutes after injection by either quick freezing (EBA injections) or fixative perfusion (ferrocyanide injections). The contents of the cranial and vertebral cavities were then examined grossly and histologically for distribution of the tracers. In all animals, the tracers were seen throughout the ventricular cavity and in the subarachnoid space surrounding the caudal hindbrain. The site of communication between these two fluid spaces was the posterior tela of the hindbrain. Within this tela, “pores” were found between groups of pavement-like ependymal cells. In many animals, tracer was also observed in the vertebral subarachnoid and epidural spaces, adjacent to spinal nerve roots. In three EBA-injected animals, this tracer was also seen in the subcardinal lymph spaces. These findings suggest that the subarachnoid space in the bullfrog communicates functionally with the ventricular system by way of specialized “pores” in the posterior tela of the hind-brain. There is also indication of movement of fluid within the subarachnoid space which is predominately caudal in direction, with a primary absorptive path for CSF that consists of a perineural route to the lymphatic system.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1091940306
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