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  • 1
    Electronic Resource
    Electronic Resource
    The contribution of solvent drag to the intestinal absorption of the acidic drugs benzoic acid and salicylic acid from the jejunum of the rat (1974)
    Springer
    Naunyn-Schmiedeberg's archives of pharmacology 281 (1974), S. 197-217 
    Details
    ISSN: 1432-1912
    Keywords: Intestinal Absorption ; Acidic Drugs ; Benzoic Acid ; Salicylic Acid ; Solvent Drag ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary 1. Jejunal loops of anaesthetized rats were perfused with hypotonic, isotonic and hypertonic buffered solutions containing 14C-labelled benzoic acid and salicylic acid at pH 6.2 and 2.2. The blood flow of the loop was maintained at an intermediate rate (0.78–0.97 ml min−1 g−1). The water net flux was determined by polyethylene glycol as non-absorbable marker and amounted up to +31 or-27 μl min−1 g−1. 2. A positive water net flux (leaving the gut lumen) increased the appearance rate of benzoic acid and salicylic acid in the intestinal venous blood by maximally 47 and 41%, a negative water net flux (entering the gut lumen) diminished the appearance rate by 28 and 37%. 3. The experimental data were analysed by means of a kinetic model. The parameters of the model were the epithelial permeability kF D , the serosal permeability k S F S and the sieving coefficient Φ=1−σ. The epithelial permeability was 0.115 and 0.107 for benzoic acid and salicylic acid at pH 6.2 and increased to 0.163 and 0.185 ml min−1 g−1 at pH 2.2. The serosal permeability was assumed to be identical for neutral and acidic pH and amounted to 0.046 and 0.112 ml min−1 g−1 indicating that a certain proportion of the absorbed drugs was transferred to the serosal side. The sieving coefficient was 2.92 and 2.31 at neutral pH and 3.79 and 3.56 at acidic pH. 4. The main resistance to the absorption of the two drugs is the epithelial membrane, since the effective blood flow and the unstirred layer are not ratelimiting. The high sieving coefficient for the two drugs is interpreted as indicating an interaction of drug and water molecules inside the lipid part of the cell membrane (which contains at least 30% water). The electric potential across the gut wall may contribute to the size of the sieving coefficient, if the drug molecules permeate also in the ionized form. 5. Using acidic perfusion solutions the epithelial permeability and the sieving coefficient were increased by the factor 1.3–1.5. This increase can be explained sufficiently by a facilitated entrance of the unionized drug molecules into the epithelial membrane.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00503498
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  • 2
    Electronic Resource
    Electronic Resource
    Dekorporierung von Thallium durch rhyolithischen Bims bei der Ratte (1974)
    Springer
    Research in experimental medicine 162 (1974), S. 63-66 
    Details
    ISSN: 1433-8580
    Keywords: Rhyolithic pumice ; Ferrihexacyanoferrate (II) ; Thallium ; Rat ; Rhyolithischer Bims ; Ferrihexacyanoferrat (II) ; Thallium ; Ratte
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Orale Verabfolgung von rhyolithischem Bims bewirkt eine herabgesetzte Retention von Thallium in den Geweben. Die Wirksamkeit von Rhyolith zeigt eine ausgeprägte Dosisabhängigkeit, ist aber geringer als die von Ferrihexacyanoferrat (II).
    Notes: Summary Oral administration of rhyolith leads to a diminished retention of thallium by the tissues. The effectiveness of rhyolith markedly depends on its dosage, but is surpassed by that of ferrihexacyanoferrate (II).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01851884
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  • 3
    Electronic Resource
    Electronic Resource
    Untersuchungen zur Beeinflussung der renalen Exkretion von D-Penicillamin bei der Ratte (1974)
    Springer
    Research in experimental medicine 163 (1974), S. 39-45 
    Details
    ISSN: 1433-8580
    Keywords: D-penicillamine ; Acetazolamide ; Renal excretion ; Rat ; D-Penicillamin ; Acetazolamid ; Renale Exkretion ; Ratte
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Die renale Ausscheidung von D-Penicillamin läßt sich weder durch Probenecid noch durch N-Methylnicotinamid oder NH4Cl beeinflussen. Acetazolamid bewirkt eine erhöhte Retention von D-Penicillamin in Haut und Muskulatur und hemmt dessen renale Ausscheidung, hat jedoch keinen Effekt auf die Dekorporationswirksamkeit von D-Penicillamin bei60Co. Die möglichen Ursachen des Einflusses von Acetazolamid auf die Verteilung und Exkretion von D-Penicillamin werden diskutiert.
    Notes: Summary The renal excretion of D-penicillamine is not influenced by probenecid, N-methylnicotinamide, and NH4Cl. Acetazolamide brings about an increased retention of D-penicillamine in skin and muscles and lowers its urinary excretion, but does not exhibit any influence on the efficacy of D-penicillamine in removing internally deposited60Co. The possible causes for the action of acetazolamide on the distribution and excretion of D-penicillamine are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01851444
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  • 4
    Electronic Resource
    Electronic Resource
    Experimental uremic syndrome in the rat (1974)
    Springer
    Research in experimental medicine 163 (1974), S. 341-349 
    Details
    ISSN: 1433-8580
    Keywords: Experimental uremic syndrome ; Rat ; 5/6 reduction of renal parenchyma ; Serum- and urine analysis ; Secondary hyperparathyroidism ; Experimentelles urämisches Syndrom ; Ratte ; 5/6-Reduktion des Nierenparenchyms ; Serum- und Urinanalyse ; Sekundärer Hyperparathyreoidismus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Durch eine 5/6-Nierenparenchymreduktion wurde an Ratten ein chronisches, durch bilaterale Nephrektomie ein akutes urämisches Syndrom erzeugt. Beim chronischen urämischen Syndrom erwiesen sich in der Serumanalyse die Konzentrationen des Harnstoffstickstoffs und des Kreatinins als wesentlich erhöht. Darüber hinaus bestanden alle chemischen Zeichen eines sekundären Hyperparathyreoidismus. Die Tiere litten außerdem an einer schweren Poly-, Protein- und Albuminurie. Überraschenderweise war aber der Spiegel des totalen Serumproteins gegenüber den Kontrolltieren erhöht. Das experimentelle chronische urämische Syndrom stellt eine extreme Adaptation der regulatorischen Aktivität des Nierenrestparenchyms und des ganzen Organismus dar. Beim akuten, durch bilaterale Nephrektomie erzeugten urämischen Syndrom besteht dagegen ein schweres Versagen der Regulation des Wasser- und Elektrolytstoffwechsels, welches innert 50–60 Std zum Tode der Versuchstiere führt.
    Notes: Summary A chronic uremic syndrome was induced by 5/6 nephrectomy and an acute uremic syndrome by bilateral nephrectomy in rats. In the chronic uremic syndrome serum and urine analysis revealed a highly increased serum level of BUN and creatinine. Furthermore, all chemical signs typical of secondary hyperparathyroidism were present. The animals suffered from severe polyuria, protein- and albuminuria but, surprisingly, the total serum protein was increased. The experimental chronic uremic syndrome is, in essence, a syndrome of “extreme adaptation” of the regulatory activity of remnant renal parenchyma and of the whole body. In contrast, the acute uremic syndrome induced by bilateral nephrectomy is a severe disorder of water and electrolyte metabolism resulting in death of the experimental animals after 50–60 hrs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01851521
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  • 5
    Electronic Resource
    Electronic Resource
    Ultrastruktur des proximalen Nierentubulus nach Behandlung mit einem Pentosanschwefelsäureester (1974)
    Springer
    Research in experimental medicine 164 (1974), S. 19-33 
    Details
    ISSN: 1433-8580
    Keywords: Kidney ; Rat ; Ultrastructure ; Proximal tubule degeneration ; Heparinoid ; Niere ; Ratte ; Ultrastruktur ; Proximale Tubulusdegeneration ; Heparinoid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung 24 Std nach i.v. Injektion von 100 mg/kg eines Pentosanschwefelsäureesters (SP 54) sind in der Nierenrinde von Ratten schwere degenerative Veränderungen der proximalen Tubuluszellen zu beobachten. Das Tubulusepithel ist flachkubisch umgewandelt, das Tubuluslumen ist angefüllt mit ausgestoßenen Mitochondrien und anderen Zelltrümmern. Am stärksten betroffen ist die Pars contorta des proximalen Tubulus. Weniger stark veränderte Tubuluszellen sind angefüllt mit Cytosomen, die eine spezifische Feinstruktur haben oder gehäuft gegenüber Kontrollen Cytoplasmaprotrusionen in das Tubuluslumen aufweisen. Die akute Tubulusdegeneration ist ein spezifischer Effekt von SP 54. Heparin bewirkt nur eine leichte Schwellung des Tubulusepithels, die häufiger auftritt als bei Kontrolltieren.
    Notes: Summary 24 hrs after the intravenous injection of 100 mg/kg of a Pentosansulfuricacidester (SP 54) severe degenerative alterations are observed in the proximal tubular cells in rat kidney cortex. The tubular epithelium has changed to a single layer of elongated cubic cells. The tubular lumen is filled with cellular debris and extruded mitochondria. The greatest change is seen in the pars contorta of the proximal tubule. Less degenerated tubular cells have a great number of cytosomes with a specific substructure or have cytoplasmatic protrusions into the tubular lumen. This acute tubular degeneration is a specific event after the administration of SP 54 and is not seen after an equimolar dosis of Heparin. Heparin induces only a slight swelling of the tubular epithelium, more frequent seen as in the control animals.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01851961
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  • 6
    Electronic Resource
    Electronic Resource
    Influence of coprophagy prevented by partial immobilization on calcium metabolism and bone morphology in the rat (1974)
    Springer
    Research in experimental medicine 162 (1974), S. 313-321 
    Details
    ISSN: 1433-8580
    Keywords: Coprophagy ; Rat ; Calcium metabolism ; Bone morphology ; Immobilization ; Koprophagie ; Ratte ; Calciumstoffwechsel ; Knochenmorphologie ; Immobilisierung
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Wir untersuchten den Einfluß der Koprophagie auf den Calciumstoffwechsel der Ratte. 20 Versuchstiere wurden durch Einschnüren in einen Lederpanzer an der Koprophagie gehindert und mit 21 Kontrolltieren gleicher Futteraufnahme verglichen. Die Kinetik des Calciumstoffwechsels wurde unter Verwendung von45Ca als Tracer analysiert. Die radiochemischen Resultate wurden mit den histologischen Beobachtungen an den Epi-Metaphysen der Kniegelenke verglichen. Bei den Versuchstieren mit verhinderter Koprophagie fanden wir: 1. ein hochsignifikant niedrigeres Körpergewicht; 2. eine hochsignifikante Verkleinerung folgender Parameter des Calciumstoffwechsels: Compartmentgröße und Austauschrate zwischen ihnen, Calciumausscheidungsraten, Knochenan- und -abbau; 3. eine Verschmälerung der Wachstumsfuge, verkürzte und verschmälerte primäre Knochenbälkchen, verminderte sekundäre metaphysäre Spongiosa. Bei 4 Versuchstieren, die in Lederpanzern gehalten, denen jedoch die Koprophagie ermöglicht war, wurden gleichartige histologische Veränderungen wie bei verhinderter Koprophagie festgestellt. Es wird gefolgert, daß die Verhinderung der Koprophagie keinen meßbaren Einfluß auf die Parameter des Calciumstoffwechsels hat.
    Notes: Summary We investigated the influence of coprophagy on the calcium metabolism of the rat. 20 experimental animals were prevented from coprophagy by wrapping them in leather harnesses and compared with 21 controls on the same food intake. Kinetics of calcium metabolism were analysed using45Ca as tracer. The radiochemical results were compared with the histological observations in the epi-metaphyses of the knee joints. In the experimental animals prevented from coprophagy we found: 1. a highly significant lower body weight; 2. a highly significant diminution of the following parameters of calcium metabolism: compartment sizes and exchange rate between them, calcium excretion rates, bone accretion and resorption; 3. a narrower epiphyseal growth cartilage, clearly shorter and narrower primary trabeculae, rarefied secondary metaphyseal trabeculae. Histological alterations identical to those observed with coprophagy prevented, were found in 4 animals kept in harnesses but with coprophagy allowed. It is concluded that prevention of coprophagy has no measurable influence on the parameters of calcium metabolism.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01851702
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  • 7
    Electronic Resource
    Electronic Resource
    Wirkung steigender Pentagastrindosen auf die Säuresekretion des Rattenmagens nach Vagotomie im Perfusionsversuch (1974)
    Springer
    Research in experimental medicine 163 (1974), S. 289-298 
    Details
    ISSN: 1433-8580
    Keywords: Gastrinpentapeptide ; Vagotomy ; Acid secretion ; Perfusion test ; Rat ; Gastrinpentapeptid ; Vagotomie ; Säuresekretion ; Perfusionsversuch ; Ratte
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Bei männlichen Wistarratten wurde eine trunkuläre beiderseitige Vagotomie — ergänzt durch eine Pyloroplastik — angelegt. 4 Wochen später erfolgte in Urethannarkose eine Perfusion des Magens mit 0,9%iger NaCl-Lösung in Anlehnung an die Versuchsanordnung von Ghosh. Die Vollständigkeit der Vagotomie bestätigte ein Hypoglykämietest. Im Abstand von 150 min erhielten die Tiere — in 6 Gruppen zu je 10 Ratten — 2 × 50, 100, 150, 200, 250 oder 300 µg/kg KG Pentagastrin subcutan. Die statistisch ausgewerteten Ergebnisse lassen sich wie folgt zusammenfassen: 1. Die nach dem Abklingen der Wirkung der ersten Pentagastrininjektion wiederholte Pentagastringabe in derselben Dosis ergab bei allen Dosierungen eine höhere Säuresekretion als nach der ersten Injektion. 2. Ein Maximum der sezernierten Säuremenge wurde bei einer Dosis von 100 µg/kg KG erreicht. Bei höherer Dosierung des Pentagastrins nahm die Menge der sezernierten Säure signifikant ab.
    Notes: Summary Male Wistar rats were subjected to a complete bilateral vagotomy and a pyloroplasty. 4 weeks later followed in urethane anesthesia a continuous perfusion of the stomach with 0.9% saline according to the rat preparation of Ghosh. The completeness of the gastric vagotomy was confirmed by a postoperative insulin test. In an interval of 150 min the rats — in 6 groups of 10 rats each — were two times injected pentagastrin subcutaneously in dose rates of 50, 100, 150, 250 or 300 µg/kg body weight. The statistical confirmed results can be summarized as followed: 1. The second injection of pentagastrin — 150 min after the first — in the same dose produced a significantly higher gastric acid secretion in the rat all dose rates in comparison to the first injection. 2. The maximal gastric acid secretion was produced by a dose rate of 100 µg/kg body weight of pentagastrin. At higher dose rates of pentagastrin there was a significant reduction of gastric acid secretion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01851516
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  • 8
    Electronic Resource
    Electronic Resource
    Functional changes of the neuronal and glial elements at the surface of the external layer of the median eminence (1974)
    Springer
    Anatomy and embryology 143 (1974), S. 255-262 
    Details
    ISSN: 1432-0568
    Keywords: Median eminence ; Neuro-vascular contacts ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The vascular surface of the external layer of the median eminence is composed of neuronal and glial processes. Neuro-vascular contacts are supposed to be necessary for the release of hypophysiotropic neurohormones. The present study confirms the hypothesis that the relative extent of neuro-vascular and glio-vascular contacts varies under different functional conditions of the tubero-hypophysial system. Morphometric analysis shows that in normal rats about 20% of the surface is lined by neuro-vascular contacts. After bilateral adrenalectomy the neuro-vascular contacts are enlarged to 40% whereas application of an extract of stalk-median eminence tissue is followed by a decrease of the neuro-vascular contacts to 15%. The implications of these results are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00519869
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  • 9
    Electronic Resource
    Electronic Resource
    Über die Entstehung der Basalmembranlabyrinthe (1974)
    Springer
    Anatomy and embryology 143 (1974), S. 185-203 
    Details
    ISSN: 1432-0568
    Keywords: Basement membrane labyrinths ; Cerebral ventricles ; Development ; Rat ; Light and Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Gehirne embryonaler und neugeborener Ratten wurden im Hinblick auf die frühe Entstehung der Basalmembranlabyrinthe licht- und elektronenmikroskopisch untersucht. 1. An den Hirngefäßen embryonaler Ratten der 2. und 3. Fetalwoche kommen zwar bereits Basalmembranen vor, sub- oder interependymale Basalmembranlabyrinthe fehlen aber noch. 2. Basalmembranlabyrinthe werden mit der Perjodsäure-Bisulfit-Aldehydthionin-Methode lichtmikroskopisch ab dem 20. Tag an umschriebenen Stellen des Ventrikelsystems gefunden; elektronenmikroskopisch treten die ersten Basalmembranlabyrinthe am 12. postnatalen Tag auf. 3. Die ersten interependymalen Labyrinthe erscheinen in Erweiterung der interependymalen Spalten in der Nähe von ausgedehnten Golgifeldern. Der Labyrinthinhalt besteht aus einer feinflockigen Substanz. Diese liegt wolkenartig angehäuft gegenüber bogenförmigen Ausbuchtungen lokal verbreiterter Zellmembranen. In Ausbildung begriffene Labyrinthe zeigen eine lamelläre Gliederung. 4. Ab dem 12. postnatalen Tag treten auch an den Basalmembranen der pericapillären Zellen, in denen lichtmikroskopisch Glykoproteide nachweisbar sind, zapfenartige Duplikaturen auf. Die Zapfen sind überwiegend ependymwärts gerichtet. Verbindungen zwischen den Basalmembranzapfen und den interependymalen Basalmembranlabyrinthen werden in den ersten 30 Lebenstagen nicht gefunden. 5. Sowohl an den die interependymalen Labyrinthe begrenzenden Plasmalemmata als auch am Plasmalemm der pericapillären Zellen fallen Stachelsaumbläschen auf, deren Membran in die Zellwand eingebaut wird. Ihr Inhalt scheint in die entstehenden Labyrinthe entleert zu werden. 6. Da sich an den tubulären Enden der Doppellamellen der Dictyosomen Strukturen befinden, die den Stachelsaumbläschen morphologisch gleichen, und da im Bereich des Golgifeldes und im Cytoplasma zwischen diesem und der Zellwand zahlreiche Stachelsaumbläschen liegen, wird angenommen, daß das Material der Basalmembranlabyrinthe dem Golgiapparat entstammt und vermittels der Stachelsaumbläschen zur Zellwand transportiert wird. Bogenförmige Ausbuchtungen lokal verbreiterter Zellmembranen im Bereich entstehender Labyrinthe werden als Reste von Stachelsaumbläschen aufgefaßt, deren Membran in die Zellwand eingebaut wurde. 7. Da die phylogenetische Zunahme des Hirnmantels mit einem Verlust der langen in das Gehirn reichenden basalen Ependymfortsätze an den größten Teilen der Ventrikelwand einhergeht und niedere Tiere noch nicht über Basalmenbranlabyrinthe verfügen, wird angenommen, daß diese die Transportfunktion für Stoffe aus dem Liquor übernommen haben, die vordem nach allgemeiner Auffassung über die langen Fortsätze in das Gehirn gelangten.
    Notes: Summary The brains of embryonic and new-born rats were investigated by means of light- and electron microscopy with regard to the early formation of basement membrane labyrinths. 1. Though basement membranes are already found around the brain capillaries of embryonic rats from the 2nd week of pregnancy, sub- and interependymal basement membrane labyrinths are still absent. 2. Basement membrane labyrinths, being demonstrable for light microscopy by a periodicacid-bisulfite-aldehydethionin-method, appear around the 20th day after birth at certain places of the ventricular system. By means of electron microscopy, basement membrane labyrinths have first been detected at the 12th postnatal day. 3. The earliest interependymal basement membrane labyrinths are found in enlargements of the interependymal spaces near a distended Golgi apparatus. The contents of the labyrinths, being composed of a loose flocculent material, are of a lamellar structure. In the intercellular space the material is situated opposite bow-shaped excavations of local broadenings of cell membranes. 4. From the 12th postnatal day, plug-like duplications of basement membranes occur at the ependymal side of the pericapillar cells, which contain glycoproteids. The plugs of basement membranes are directed towards the ependymal layer. No connections between the interependymal basement membrane labyrinths and the plugs of pericapillary basement membranes exist within the first 30 days of life. 5. At the plasmalemma of ependymal cells bordering the interependymal labyrinths, and at the cell membrane of pericapillary cells, coated vesicles are to be found, which are fused with the cell membrane. The contents of these vesicles seem to be released into the developing labyrinths. 6. At the tubular ends of dictyosomes, coated vesicle-like structures can be demonstrated. In the environment of the Golgi apparatus many coated vesicles are situated; they can even be found between the Golgi apparatus and the walls of labyrinthś. Therefore the coated vesicles are considered to be transport vesicles, transporting the material which is formed in the Golgi apparatus towards the cell membrane. 7. Since the phylogenetic increase of the brain mantle is accompanied by loss of the long processes of ependymal cells that reach far into the brain, and since lower animals have no basement membrane labyrinths, it is suggested that the basement membrane labyrinths have a transport functions for material from the cerebrospinal fluid which in lower animals is assumed to be transported by the long processes of ependymal cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00525769
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  • 10
    Electronic Resource
    Electronic Resource
    Feinbau und Passageverhalten der Capillaren im Subcommissuralorgan der Ratte (1974)
    Springer
    Anatomy and embryology 144 (1974), S. 101-122 
    Details
    ISSN: 1432-0568
    Keywords: Subcommissural organ ; Periodically structured bodies ; Vascular permeability ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Die Capillaren des Subcommissuralorgans (SCO) und deren Umgebung wurden an 38 adulten Sprague-Dawley- und an 6 adulten Wistar-Ratten nach Perfusionsfixierung im Elektronenmikroskop untersucht. Saure Mucopolysaccharide wurden mit Alcianblau und Rutheniumrot, Glykoproteide mittels der Perjodsäure-Silbermethenamin-Reaktion nachgewiesen. Der Stofftransport in den Capillaren wurde mit Meerrettichperoxidase (MRP) als Tracer untersucht. Die subcommissuralen Capillaren besitzen ein ungefenstertes, vesikelarmes Endothel, das einer einfachen, durchgehenden Basallamina aufsitzt. In der Umgebung der Capillaren finden sich neben periodisch strukturierten Körpern (PSK) vereinzelt Basallamina-Labyrinthe und regellos angeordnete kollagene Mikrofibrillen. Mit der Perjodsäure-Silbermethenamin-Reaktion lassen sich Glykoproteide im Bereich der Basallamina und der nach außen anschließenden Intercellularspalten darstellen, vereinzelt auch — teils im Bereich der Basallamina, teils ihr eng angeschlossen — Gruppen parallel angeordneter, linearer Strukturen mit einem periodischen Streifenmuster. Saure Mucopolysaccharide sind nur in der lumenseitigen Glykokalyx des Capillarendothels nachweisbar. Der Aufbau der PSK aus Kollagenfilamenten erscheint sehr wahrscheinlich. Vermutungen zur Entstehung dieser hochgeordneten Strukturen werden vorgebracht. Die Traceruntersuchungen ergeben, daß unter normalen Bedingungen in den subcommissuralen Capillaren eine Blut-Hirn-Schranke (BHS) für MRP besteht, die im Endothel lokalisiert ist. Bei einem Teil der Sprague-Dawley-Ratten ist die Barriere geschädigt und der Tracer gelangt über cytopemptische Vesikel in Basallamina und Gefäßumgebung. Der Basallamina und den PSK kommt in solchen Fällen keine besondere Schranken- oder Verteilerfunktion zu. Offenbar bewirkt bei manchen Sprague-Dawley-Ratten die MRP-Injektion über die Freisetzung von endogenen biogenen Aminen aus Mastzellen eine Schädigung der BHS, was bei Wistar-Ratten nicht eintritt.
    Notes: Summary The capillaries of the subcommissural organ (SCO) and their adjacent structures of 38 adult Sprague-Dawley rats and 6 adult Wistar rats were examined in the electron microscope after perfusion fixation. Acid mucopolysaccharides were shown by Alcian Blue and Ruthenium Red, glycoproteins were identified by the periodic acid-silver methenamine technique. Horseradish peroxidase (MRP) was used as a tracer for vascular permeability. The subcommissural organ's capillaries are characterized by an unfenestrated, continuous endothelium, showing only few vesicles; the endothelium is surrounded by a single, continuous basal lamina. Adjacent to the capillaries wall there are “periodically structured bodies” (PSK), labyrinths of the basal lamina and irregularly arranged collagen fibrils. Glycoproteins are found in the basal lamina and within the intercellular clefts; in a few cases groups of parallel arranged, periodically banded linear structures are seen within or adjacent to the basal lamina. Acid mucopolysaccharides are found within the endocapillary layer of capillaries. It seems very likely that PSK represent an atypical arrangement of collagen fibrils. The formation of these structures is discussed. Under normal conditions, MRP, after intravenous injection, does not enter the perivascular space surrounding the capillaries of the SCO. A blood-brain barrier exists at the level of the capillary endothelium. In a few Sprague-Dawley rats MRP seems to alter the permeability of the capillaries. Reaction product of MRP is found within endothelial vesicles and the adjacent structures. It appears that the basal lamina and the PSK are not involved in the barrier mechanism or regulation of transport. In Sprague-Dawley rats intravenous injection of MRP seems to alter the blood-brain barrier by release of endogenous biogenic amines associated with mast cell degranulation. This effect is not observed in Wistar rats.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00518636
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  • 11
    Electronic Resource
    Electronic Resource
    Changes of extent of Neuro-vascular contacts and number of neuro-glial synaptoid contacts in the pituitary posterior lobe of dehydrated rats (1974)
    Springer
    Anatomy and embryology 146 (1974), S. 157-165 
    Details
    ISSN: 1432-0568
    Keywords: Neurohypophysis ; Neuro-vascular contacts ; Neuro-glial synaptoid contacts ; Dehydration ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The posterior lobe of the hypophysis consists of neurosecretory nerve fibres and glial cells, both abutting on the perivascular space of sinusoidal capillaries. Nerve fiberes and glial cells are connected with each other by a high number of synaptoid contacts. Several ultrastructural changes following dehydration have been described by other authors. This study presents the data of some morphometric investigations on normal and dehydrated rats. The relative extent of neuro-vascular contacts is not contstant; it increases significantly from 52 (±2.8)% in normal rats to 67(±1.2)% in rats with a dehydration time of 3 days. Correspondingly the extent of glio-vascular contacts is diminished. In addition, water-deprived animals show a clear increase in the number of neuro-glial synaptoid contacts with 3.1 (±0.4) per square unit nervous tissue compared to 2.1 (±0.3) per Square unit in untreated controls. These results indicate a dynamic motility of neuronal and glial elements depending on functional conditions, as was found for the external layer of the median eminence. Furthermore there seems to be a relationship between secretory activity and the number of neuroglial synaptoid contacts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00315592
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  • 12
    Electronic Resource
    Electronic Resource
    Electron microscopic studies of the innervation of the rat kidney (1974)
    Springer
    Anatomy and embryology 145 (1974), S. 169-186 
    Details
    ISSN: 1432-0568
    Keywords: Renal innervation ; Rat ; Peripheral nerves ; Neuroeffector zones ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung In der Rattenniere werden die muskelzellhaltigen arteriellen Gefäße und der juxtaglomeruläre Apparat innerviert. Blutgefäße mit Pericyten, porenhaltige Capillaren sowie die Tubuli der Rinde und des Markes werden nicht von Nervenfasern begleitet. Ganglienzellen wurden in der Rattenniere nicht beobachtet. Periphere Nerven mit einem ein-bis zweischichtigen Perineurium kommen im paravasalen Gewebe der Interlobar- und Arcuata-Arterien vor; sie enthalten neben zahlreichen marklosen Nervenfasern gewöhnlich auch 2–4 markhaltige. Nervenfaser-Bündel ohne perineurale Scheide finden sich im paravasalen Gewebe der Arcuata- und Interlobular-Arterien. Darüber hinaus sind in unmittelbarer Nachbarschaft der großen Arterien (Interlobar-, Arcuata- und Inter-lobular-arterien) und der Vasa afferentia marklose Nervenfasern und freie Axone vorhanden, die auch die proximalen Abschnitte der Vasa efferentia der subcapsulären und intermediären Rindenschicht begleiten. Im Nierenmark werden die juxtamedullären Vasa efferentia und die Arteriolae rectae innerviert; marklose Nervenfasern und freie Axone sind nur bis zur Außen-Innenstreifen-Grenze nachweisbar. Die Innervation der muskelzellhaltigen arteriellen Gefäße erfolgt durch aufgetriebene Axonabschnitte (Neuroeffektor-Zonen), die vorwiegend agranuläre Vesikel enthalten. Diese Strukturen liegen stets an der Grenze von Adventitia und Media bzw. Elastica externa; zwischen den glatten Muskelzellender Media wurden keine vesikelhaltigen Axonabschnitte gefunden. Als minimaler Abstand zwischen den vesikelhaltigen Axonabschnitten und den von einer Basalmembran umschlossenen glatten Muskelzellen (neuromuskuläre Distanz) wurden 600 Å gemessen. Die vorliegenden Ergebnisse werden mit den fluoreszenzmikroskopischen und histochemischen Untersuchungen über die adrenerge und cholinerge Innervation der Niere verglichen. Die sich aus diesem Vergleich ergebenden Probleme und funktionellen Konsequenzen für die Innervation der Niere sowie die Natur der cholinergen Fasern (afferente oder postganglionäre parasympathische Fasern) werden diskutiert.
    Notes: Summary The innervation of the rat kidney is defined by a system which supplies those arterial blood vessels whose walls contain smooth muscle cells and the juxtaglomerular apparatus. Vessels containing pericytes, or those vessels composed of an endothelium only, as well as the tubules of both the cortex and medulla, are not innervated. Furthermore, ganglion cells do not occur in the rat kidney. The nervous apparatus of the rat kidney consists of peripheral vegetative nerves, ensheathed by a perineurium, with 2–4 myelinated fibers running in the paravasal tissue of the interlobar and arcuate arteries, and of nerve bundles without a perineurial sheath in the paravasal tissue of the arcuate and interlobular arteries. Non-myelinated fibers and free axons occur in the immediate vicinity of the great arteries (interlobar, arcuate, and interlobular) and the vasa afferentia. Nerve fibers and free axons are also seen in the vicinity of only the proximal parts of those vasa efferentia which supply the cortical capillary plexus. The arteriolae rectae of the medulla, and their vasa efferentia, from which they arise, are innervated by non-myelinated fibers and free axons which accompany these arterial vessels only to the boundary of the outer and inner stripe of the outer zone of the medulla. The functional innervation of those vessels with smooth muscle cells results from neuro-effector zones which predominantly show agranular vesicles. These structures were never seen between the smooth muscle cells within the media; the minimum neuromuscular distance was 600 Å. The present findings are correlated with the lightmicroscopically demonstrated adrenergic and cholinergic innervation. The resultant problems and functional consequences of the innervation of the kidney, especially the nature of the cholinergic fibers (afferent or post-ganglionic parasympathetic fibers) are briefly discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00519727
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  • 13
    Electronic Resource
    Electronic Resource
    Neuroembryogenesis in the hippocampal formation of the rat (1974)
    Springer
    Anatomy and embryology 144 (1974), S. 173-186 
    Details
    ISSN: 1432-0568
    Keywords: Hippocampus ; Neurogenesis ; Autoradiography ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summay The prenatal ontogeny of the hippocampus, including the anterior continuation and induseum griseum, was examined in the rat by means of tritiated thymidine autoradiography. The neurons of the anterior continuation formed between the 16th and 18th days of embryonic development. The neurons of the induseum griseum were formed on the 16th and 17th days of gestation. The pyramidal cells of ammon's horn were formed during the 16th, 17th, 18th, and 19th days of embryogenesis but were preceded by the large interneurons of the stratum lacunosum-moleculare which formed on days 15 and 16 of embryonic life. The granule cells of the superficial layers of the stratum granulosum formed during the 20th, 21st, and 22nd days of gestation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00519773
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  • 14
    Electronic Resource
    Electronic Resource
    Morphological types of motor nerve terminals in rat hindlimb muscles, possibly innervating different muscle fiber types (1974)
    Springer
    Anatomy and embryology 144 (1974), S. 237-247 
    Details
    ISSN: 1432-0568
    Keywords: Motor nerve terminals ; Methylene blue stain ; Hindlimb muscles ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary With methylene blue stain, three morphological types of motor nerve terminals were distinguished in hindlimb muscles (extensor digitorum longus, semitendinosus, soleus, and tibialis anterior muscles) of rat. Type A terminals possess many long thin branches with numerous minute swellings, type C ones possess few and thick terminal branches with few large swellings, and type B ones possess intermediate characteristics. The frequencies of occurrence of these types are characteristic for each muscle and differ between different hindlimb muscles. Specifically, soleus muscle contains a much higher proportion of type B terminals than other hindlimb muscles, and the superficial part of tibialis anterior muscle contains a higher proportion of type A terminals and a lower proportion of type C ones than the deep part. The frequencies of occurrence of types A, B, and C terminals closely parallel the frequencies of “white”, “intermediate”, and “red” muscle fibers (as determined by a fat stain), respectively, in the different muscles. As also suggested previously for the diaphragm, it is concluded that three morphological types of motor nerve terminals each may innervate one of three types of muscle fibers.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00522809
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  • 15
    Electronic Resource
    Electronic Resource
    Influence of pantothenic acid deficiency on the amount of CRF-granules in the rat median eminence (1974)
    Springer
    Anatomy and embryology 146 (1974), S. 43-55 
    Details
    ISSN: 1432-0568
    Keywords: Pantothenic acid deficiency ; Corticotropin-releasing factor ; Corticoids ; Histologie ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In the outer layer of the rat median eminence “Gomori-positive” granules occur which are assumed to be the morphological correlate of the corticotropin-releasing factor (CRF). The present study was carried out in order to establish whether the amount of these CRF-granules is influenced by a pantothenic acid (PA)-deficiency, which is known to result in an inhibition of corticosteroid production. Female SPF Wistar rats were used. PA-deficiency was induced by PA-free nutrition and/or administration of the PA-antagonist Ω-methyl-PA. Corresponding experimental groups were given either tap water or a solution of 1% NaCl and 5% glucose in tap water to drink. In rats fed with a diet free of PA and in addition treated with Ω-methyl-PA a severe disturbance of the corticoid balance developed within 6 weeks. The corticosterone production was depressed to about 11–16% of the control values. The adrenal cortex showed necrotic changes which either involved the whole parenchyma or were confined to the inner zones. In those animals in which a subcapsular region, in its extent corresponding to the zona glomerulosa, remained intact, an augmentation of the CRF-granules was observed. In those animals, however, in which the whole adrenal cortex was damaged, the amount of CRF-granules did not increase. Administration of the 1% NaCl containing drinking fluid did not influence the augmentation of the CRF granules, but had a protective effect on the development of necrotic changes in the zona glomerulosa of the adrenal cortex. The findings show that a functional relationship exists between the inner zones of the adrenal cortex and the CRF-granules in the median eminence.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00341382
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  • 16
    Electronic Resource
    Electronic Resource
    Scanning electron microscopy demonstration of cilia in rat intrahepatic bile ducts (1974)
    Springer
    Anatomy and embryology 145 (1974), S. 223-226 
    Details
    ISSN: 1432-0568
    Keywords: Rat ; Liver ; Bile ducts ; Cilia ; Scanning Electron Microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Numerous cilia have been demonstrated by SEM in cells of rat perilobular and portal bile ducts and ductules. Generally these cilia appear as long, cylindrical evaginations of the plasma membrane. Some of them are so long that, curving and twisting at many points, they cross the ductal lumen. It has been suggested that they may be related to a continual mixing up and propulsion of the bile product down the biliary tree.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00519634
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  • 17
    Electronic Resource
    Electronic Resource
    The fine structure of the pulmonary stretch receptor in the rat (1974)
    Springer
    Anatomy and embryology 143 (1974), S. 215-222 
    Details
    ISSN: 1432-0568
    Keywords: Lung ; Stretch receptors ; Light microscopy ; Electron microscopy ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Lappenbronchien (3./6.) (Durchmesser bei mittlerer Inspirationslage 500–700 μ) von männlichen und weiblichen Wistarratten wurden licht- und elektronenmikroskopisch untersucht. Diese Bronchien entsprechen im Wandbau den mittleren Bronchioli größerer Säugetiere. Freie, verzweigte lanzettförmige Terminalfasern werden im Bindegewebe der lamina propria beschrieben. Sie sind in das elastisch muskuläre System der Bronchuswand eingebaut. Die zugehörige markhaltige afferente Nervenfaser ist verzweigt und hat einen Durchmesser von 4–6 μ. Außerdem werden efferente motorische Endigungen an der glatten Bronchialmuskulatur beschrieben. Die freien lanzettförmigen und verzweigten Terminalfasern sind möglicherweise Dehnungsrezeptoren für den Hering Breuer Reflex.
    Notes: Summary In rats the 3rd to 6th bronchi, measuring 500–700 μ in diameter during inspiration, were investigated by light and electron microscopy. The histological appearance of these bronchi is comparable to that of medium sized bronchioles of larger animals. The branched and lanceolate terminals are associated with the connective tissue of the lamina propria and the smooth muscle cell layer. In this way the terminals are bound to the myoelastic system of the bronchial wall. The myelinated afferent fiber is branched and the diameter measures about 4–6 microns. Besides afferent nerve terminals these are numerous efferent endings on the smooth muscle basement laminae. It is supposed that the described receptor represents the pulmonary stretch receptor of the Hering Breuer reflex.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00525771
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  • 18
    Electronic Resource
    Electronic Resource
    Ganglienzellen und paraganglionäre Zellen des Ganglion cervicale superius der Ratte nach Parachlorophenylalanin (pCPA) (1974)
    Springer
    Anatomy and embryology 143 (1974), S. 223-237 
    Details
    ISSN: 1432-0568
    Keywords: Superior cervical ganglion ; Rat ; p-Chlorophenylalanine ; 5-Hydroxytryptamine ; Catecholamine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Die Wirkung von Parachlorophenylalanin (pCPA) auf die paraganglionären Zellen und sympathischen Ganglienzellen des Ganglion cervicale superius der Ratte wurde untersucht. pCPA beeinflußt die sympathischen Nervenzellen im Ganglion cervicale superius geringgradig. Es finden sich unspezifische Veränderungen des Golgi-Apparates und der Nissl-Schollen sowie eine Zunahme von Lysosomen. Bei langdauernder Behandlung degenerieren einzelne Ganglienzellen und Nervenfasern. Die paraganglionären Zellen sind bei Kontrolltieren durch “dense core vesicles” eines einheitlichen Typs charakterisiert. Im Laufe der pCPA-Behandlung finden sich degranulierte paraganglionäre Zellen. Die Degranulation der einzelnen Zellelemente ist jedoch variabel. Bei fluorescenzmikroskopischer Untersuchung zeigt sich nach pCPA-Applikation eine Verschiebung des Fluorescenzspektrums zum grünen Bereich hin. Die bei Kontrollen deutliche Gelbkomponente nimmt nach 6–12 Tagen ab und verschwindet schließlich nach 18–20 Tagen. Es wird diskutiert, ob die fluorescenzmikroskopischen Veränderungen aufgrund einer Hemmung der Serotoninsynthese oder aufgrund einer abgeschwächten Katecholaminsynthese entstehen.
    Notes: Summary The effect of parachlorophenylalanine (pCPA) on the paraganglionic and sympathetic nerve cells of the rat superior cervical ganglion was studied. pCPA influences the sympathetic nerve cells of the cervical ganglion to minimal extent. Unspecific changes of the Golgi apparatus and of the Nissl bodies as well as an increase in the number of lysosomes occur. An extended period of treatment causes degeneration of individual ganglionic cells and nerve fibers. The paraganglionic cells in control animals are characterized by dense core vesicles of an unitary type. During pCPA treatment degranulated paraganglionic cells are to be found. The degranulation of the individual cell elements varies. Fluorescence microscopic study after pCPA application shows a shift of the fluorescence spectrum toward green. The yellow component of the control group decreases after 6 to 12 days and disappears after 18 or 20 days. It is discussed whether the fluorescence microscopic changes are due to the inhibition of serotonine synthesis or to a decrease of the catecholamine synthesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00519867
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  • 19
    Electronic Resource
    Electronic Resource
    Effects of triparanol and 20,25-diazacholesterol in CNS of rat: Morphological and biochemical studies (1974)
    Springer
    Acta neuropathologica 29 (1974), S. 141-156 
    Details
    ISSN: 1432-0533
    Keywords: Cholesterol Inhibitors ; Cerebrum ; Neuronal Inclusions ; Oligodendroglial Degeneration ; Rat ; Abnormal Sterols
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Wistar strain of rats received daily intraperitoneal or oral administration of Triparanol, 250 mg per kg of body weight or 20,25-diazacholesterol either 30 mg or 60 mg per kg of body weight starting at the age of 5 days old. Prominent intracytoplasmic osmiophilic inclusions identical to those found in the rats treated with AY9944 (Suzukiet al., 1973) and degeneration of myelin sheaths and oligodendroglia were seen in the myelinating white matter of rats sacrificed after 3, 6, and 9 injections of Triparanol. In diazacholesterol-treated animals, a few identical inclusions were seen in the cortical neurons but only slight changes were seen in oligodendroglia. Biochemical analysis of the brains of the rats receiving Triparanol revealed a large accumulation ofΔ 7,24-cholestadiene-3β-ol and lesser amounts of desmosterol. The brains of the animals treated with diazacholesterol revealed massive accumulation of desmosterol and a minor sterol component, probablyΔ 5,7,24-cholestatriene-3β-ol. Since AY9944, which results in a massive accumulation of 7 dehydrocholesterol (Δ 5,7-cholestadiene-3β-ol) and two other sterols,Δ 7,24-cholestadiene-3β-ol andΔ 5,7,24-cholestatriene-3β-ol, will produce identical neuropathological lesions to those seen in Triparanol treated animals, and since the lesion produced in diazacholesterol treated animals was very slight, there appears to be a correlation between the presence of sterols with a double bond at the 7-position and neuropathological lesions in young rats.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00684773
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  • 20
    Electronic Resource
    Electronic Resource
    The morphology of experimental tumours of the nervous system (1974)
    Springer
    Acta neuropathologica 27 (1974), S. 25-32 
    Details
    ISSN: 1432-0533
    Keywords: Experimental CNS Tumours ; Nitrosomethylurea ; Ethylnitrosourea ; Primary Cultures ; Tumour Classification ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary By means of the explant technique a series of primary tumours induced by methylnitrosourea (MNU) or ethylnitrosourea (ENU) in the brain of rats were cultured in vitro. The morphology of isomorphic oligodendrogliomas, astrocytomas, glioblastomas and sarcomas under in vitro conditions is described. All these neoplasias show a very distinct morphological picture and growth behaviour, thus permitting their classification. Most of the tumours tend to anaplastic changes during longer cultivation, but in some cases, preferably in well differentiated gliomas, the cytological properties are maintained even during longterm cultivation. The role of anaplasia and intermingling of different neoplastic cell types in experimental tumours are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00687237
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  • 21
    Electronic Resource
    Electronic Resource
    Experimental allergic encephalomyelitis in the rat: Histopathological studies and the effects of sera on adult cerebellar organ cultures (1974)
    Springer
    Acta neuropathologica 27 (1974), S. 83-91 
    Details
    ISSN: 1432-0533
    Keywords: Allergic Encephalomyelitis ; Organ Culture ; Brain ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Experimental allergic encephalomyelitis (EAE) developed in 15 of 30 rats injected with a mixture of Freund's adjuvant and homogenized guinea pig brain, and killed after 6–29 days. The incidence and severity of the disease increased with time after inoculation and in 2 animals involvement of the epiphysis, not previously reported in EAE, was detected. Toxic effects on cells in cultured adult rat cerebellar cortical tissue were produced by exposure for 24 h to the sera of rats killed 12–29 days after inoculation either with the antigenic suspension or with adjuvant alone. Severe toxicity to axons (widespread fragmentation) was produced only by the sera of animals injected with antigenic inocula and killed 17–29 days later. Severe axonal toxicity was not, however, correlated with the presence of EAE in the rats from which the sera were taken. It was not possible to assess myelinotoxic effects in the system used. It is suggested that the organ culture technique may detect the presence in the sera of inoculated animals of toxic principles which, owing to the blood-brain barrier, do not normally contribute to the pathogenesis of EAE.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00687243
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  • 22
    Electronic Resource
    Electronic Resource
    Ultrastructural alterations of rat brain microglial cells and pericytes after chronic lead poisoning (1974)
    Springer
    Acta neuropathologica 28 (1974), S. 25-35 
    Details
    ISSN: 1432-0533
    Keywords: Chronic Lead Intoxication ; Rat ; Ultrastructure ; Microglia ; Pericytes ; Monocytes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Wistar rats of both sexes and the same litter were exposed to chronic lead intoxication from birth untill sacrifice 9 months later. Lead was administered as 0.4% solution of lead nitrate in drinking water. Samples from the parietal brain cortex were examined electron microscopically following intracardiac perfusion with paraformaldehyde-glutaraldehyde solution. Similar changes were observed in the microglial cells and the vascular pericytes whereas all the other tissue elements appeared intact. Both cell types hypertrophied, the microglia assumed characteristic spindle or rod shape, the cell organelles increased, the microglial endoplasmic reticulum widened strongly and a large number of lipid inclusions appeared. The latter consisted of large lipid droplets of varying size and shape, containing multiple zones of low density, and a dense component with a coarse granular structure. The similarity in the response of both cell entities to the lesion as well as some probable functions of microglial cells and their relationship to vascular pericytes are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00687515
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  • 23
    Electronic Resource
    Electronic Resource
    The effects of sodium fluoride on bone breaking strength (1974)
    Springer
    Calcified tissue international 14 (1974), S. 283-289 
    Details
    ISSN: 1432-0827
    Keywords: Sodium fluoride ; Osteoporosis ; Bone ; Strength ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Description / Table of Contents: Résumé L'utilisation thérapeutique de fluorure de sodium a été recommandée dans le traitement de diverses maladies osseuses ostéopéniques. Cet emploi est basé principalement sur les effects ostéosclérotiques bien connus du fluorure de sodium, mais on connait mal son effet sur la force de résistance osseuse. L'influence de diverses concentrations de fluorure de sodium sur la force de résistance osseuse de jeunes rats soumis à des régimes riches et pauvres en calcium a été étudiée. L'administration de fluorure de sodium augmente le diamètre osseux, indiquant une stimulation de la formation de l'os périosté, mais la force de résistance osseuse est réduite ou non modifiée par l'ingestion de fluor.
    Abstract: Zusammenfassung Die therapeutische Verwendung von Natriumfluorid ist für eine Anzahl von Knochenmangel-Krankheiten empfohlen worden. Die Empfehlungen basieren hauptsächlich auf den bekannten osteosklerotischen Wirkungen von Natriumfluorid; über dessen Effekt auf die Knochenstärke ist wenig bekannt. In dieser Arbeit wurde der Einfluß verschiedener Konzentrationen von Natriumfluorid auf die Knochenstärken von wachsenden Ratten mit hoher und niederer Calciumeinnahme untersucht. Die Verabreichung von Natriumfluorid erhöhte den Knochendurchmesser, was auf eine Stimulierung der Periostbildung hinwies, die Knochenstärke wurde jedoch durch Fluorideinnahme herabgesetzt oder nicht beeinflußt.
    Notes: Abstract The therapeutic use of sodium fluoride has been recommended in a variety of osteopenic bone diseases. The recommendations are based mainly on the known osteosclerotic effects of sodium fluoride and little information is available as to its effect on bone strength. The influence of various concentrations of sodium fluoride on bone strength in growing rats on high and low calcium diets was studied. The administration of sodium fluoride increased bone diameter, indicating stimulation of periosteal bone formation, but bone strength was reduced or not affected by fluoride ingestion.
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    URL: http://dx.doi.org/10.1007/BF02060302
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  • 24
    Electronic Resource
    Electronic Resource
    Special aspects of firing rates and thermosensitivity of preoptic neurons (1974)
    Springer
    Experimental brain research 21 (1974), S. 107-112 
    Details
    ISSN: 1432-1106
    Keywords: Preoptic units ; Thermosensitivity ; Mean firing rate ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Mean firing rates (per 10 sec) of preoptic neurons of the rat were recorded at normal temperature and after central warming and cooling. Thermosensitivity was determined according to the reactions of the mean firing rate. In compatibility with results from other animals we obtained the following percentages (52 neurons): 23% warm-sensitive, 13% cold-sensitive, 64% non-thermosensitive neurons. However, it turned out that a lot of interesting special effects are not taken into account, using exclusively the criterion “increasing/decreasing/constant mean firing rate”. Particularly we observed long lasting adaption processes and stationary non-linear effects as well as striking oscillations which were dependent on the thermal stimuli, while the mean interval value remained constant.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00234261
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  • 25
    Electronic Resource
    Electronic Resource
    The contribution of solvent drag to the intestinal absorption of the basic drugs amidopyrine and antipyrine from the jejunum of the rat (1974)
    Springer
    Naunyn-Schmiedeberg's archives of pharmacology 281 (1974), S. 175-196 
    Details
    ISSN: 1432-1912
    Keywords: Intestinal Absorption ; Basic Drugs ; Amidopyrine ; Antipyrine ; Solvent Drag ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary 1. Jejunal loops of anaesthetized rats were perfused with hypotonic, isotonic and hypertonic buffered solutions containing 14C-labelled amidopyrine and antipyrine at neutral and at acidic pH. The blood flow of the loop was maintained at an intermediate rate (0.7–1.0 ml min−1g−1). The water net flux was determined by means of polyethylene glycol as non-absorbable marker and amounted up to ±30 μl min−1g−1. 2. A positive water net flux (directed towards the blood) increased the appearance rate of amidopyrine and antipyrine by maximally 43.8 and 49.2%, a negative water net flux (directed towards the gut lumen) diminished it by 38.8 and 35.0%. 3. The experimental data were analysed by means of a kinetic model with the water net flux as independent variable and the epithelial permeability kF D , the serosal permeability k S F S and the sieving coefficient Φ=1−σ as absorption parameters. For antipyrine independent of the pH-value of the perfusion solution kF D was 0.123, and, for amidopyrine at pH 7 and pH 3 kF D was 0.231 and 0.091, respectively. k S F S was zero indicating that in this experimental arrangement the transfer of drug molecules to the serosal side was negligible. The sieving coefficient Φ amounted to 2.30 for amidopyrine and 2.15 for antipyrine at neutral pH. At acidic pH it amounted to 0.50 for amidopyrine and 1.44 for antipyrine. The hydraulic permeability of water was identical at neutral and acidic pH. 4. The high sieving coefficient for the two drugs at neutral pH is interpreted as indicating that water and lipophilic drug molecules interact within the lipid part of the cell membrane. At acidic pH the ionized drug molecules appear to permeate the cell membrane preferentially across other (presumably more hydrophilic) areas.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00503497
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  • 26
    Electronic Resource
    Electronic Resource
    The influence of blood flow on the phlorizine-insensitive and sensitive galactose absorption in rat jejunum (1974)
    Springer
    Naunyn-Schmiedeberg's archives of pharmacology 282 (1974), S. 195-212 
    Details
    ISSN: 1432-1912
    Keywords: Intestinal Blood Flow ; Intestinal Absorption ; d-Galactose ; Phlorizine ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary 1. Jejunal loops of anaesthetized rats were perfused with buffered solutions (pH 6.8) containing d-galactose in two concentrations: below (100 μM) and above (100 mM) blood sugar concentration. The blood flow rate, the appearance rate (in the intestinal venous blood) and the disappearance rate (from the intestinal lumen) were determined simultaneously, the accumulation in gut tissue at the end of the experiments. 2. At constant intermediate blood flow the appearance rate and disappearance rate decreased with time. A decrease of the blood flow rate from about 1.9 to about 0.3 ml min−1 g−1 diminished both the appearance and the disappearance rate. An increase of blood flow from about 0.3 to about 1.9 ml min−1 g−1 did not increase the absorption rates; even a slight decrease was observed. 3. In the presence of phlorizine (2 mM) the absorption rates and the tissue accumulation of galactose is reduced. At constant intermediate blood flow the appearance and disappearance rate increased with time. A decrease of the blood flow rate from about 1.8 to about 0.3 ml min−1 g−1 diminished the appearance rate. An increase of the blood flow rate from about 0.3 to about 1.8 ml min−1 g−1 caused an increase of the appearance rate in contrast to the experiments without phlorizine. Therefore, the different blood flow dependence of the intestinal absorption observed with actively transported substances can be attributed to their non-passive component.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00499034
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    Electronic Resource
    Fenfluramine-induced ultrastructural alterations in tissues of rats and guinea pigs (1974)
    Springer
    Naunyn-Schmiedeberg's archives of pharmacology 285 (1974), S. 175-184 
    Details
    ISSN: 1432-1912
    Keywords: Fenfluramine ; Rat ; Guinea Pig ; Phospholipidosis, drug-induced ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary After administration of the anorectic drug fenfluramine (40 mg/kg per day) to rats (s. c. or i. p.) and guinea pigs (p.o.) over periods of 2 to 11 weeks, electron microscopic examination was performed on lungs, liver, lymphatic tissues and peripheral blood of both species, and on adrenal glands and ovaries of rats. In rats, fenfluramine caused the following alterations (listed according to the duration of treatment): Formation of abnormal lamellated inclusions in lymphocytes and plasma cells of lymphatic tissues, appearance of “foam cells” in lung alveoli; formation of abnormal lamellated inclusions in corpus luteum, lymphocytes of peripheral blood, and in adrenal cortex. In guinea pigs, the same alterations were found in lymphocytes of spleen and peripheral blood, in hepatocytes and in lung. The present observations support the concept of a generalized phospholipidosis induced by amphiphilic compounds. The potency of fenfluramine to induce a lipidosis is, however, considerably less pronounced than that previously demonstrated for the anorectic drug chlorphentermine. This difference is suggested to be due mainly to the lower degree of amphiphilia of fenfluramine.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00501152
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  • 28
    Electronic Resource
    Electronic Resource
    The action of chlorpromazine on exploration in pairs of rats (1974)
    Springer
    Psychopharmacology 37 (1974), S. 249-254 
    Details
    ISSN: 1432-2072
    Keywords: Chlorpromazine ; Rat ; Social Interaction ; Exploration ; General Motor Activity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract When two animals were placed in a hole-board the number of headdips made by each rat was twice the number made when tested alone, but was the same for pairs of drugged (2 mg/kg chlorpromazine) and undrugged rats, and for pairs where only one rat was drugged. This contrasts with the reduction of headdipping produced by chlorpromazine in single animals. However, chlorpromazine still reduced general motor activity, regardless of whether the partner was drugged or not. The activity of the undrugged rats was affected by the partner's state and was lower when the partner was drugged.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00421538
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  • 29
    Electronic Resource
    Electronic Resource
    Effects of low and high doses of l-Dopa on the tetrabenazine or α-methyltyrosine-induced suppression of behaviour in a successive discrimination task (1974)
    Springer
    Psychopharmacology 39 (1974), S. 199-212 
    Details
    ISSN: 1432-2072
    Keywords: Rat ; Successive Discrimination ; Conditioned Avoidance Response ; Nerve Impulses ; α-Methyltyrosine ; Tetrabenazine ; l-Dopa
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The effect of the administration of l-Dopa, 10 or 100 mg/kg i.p., on the α-methyltyrosine methylester HCl (α-MT; 250+50 mg/kg i. p.) or tetrabenazine (TBZ; 2 mg/kg i.p.)-induced suppression of a successive discrimination-conditioned avoidance task has been studied. It was found that administration of the high l-Dopa dose resulted in a reversal of the α-MT- or TBZ-induced suppression of the avoidance behaviour, although the discrimination was lost. Restoration of the avoidance behaviour and the correct discrimination was obtained with the low l-Dopa dose after α-MT but not after TBZ. The difference in the behavioural response to l-Dopa after α-MT as compared to the response obtained after TBZ is discussed in terms of the availability of the catecholamines formed from l-Dopa for release by the nerve impulses. Biochemical determinations of brain noradrenaline and dopamine were made in parallel. Further, a simple and reliable method for the aquisition of a successive discrimination in the rat is described.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00421027
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  • 30
    Electronic Resource
    Electronic Resource
    Action of a new psychotropic drug (Sulpiride) on avoidance behavior in rats (1974)
    Springer
    Psychopharmacology 39 (1974), S. 309-314 
    Details
    ISSN: 1432-2072
    Keywords: Avoidance ; Drug ; Psychotropic ; Rat ; Sidman Schedule ; Sulpiride
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Sulpiride, a new psychotropic drug with neuroleptic activity, was administered to rats in a Sidman avoidance schedule. Doses ranging from 1 to 80 mg/kg resulted in the animal receiving fewer shocks. This effect was not correlated with an increase of responding and seemed unrelated to the suppressing action abruptly observed at high doses (i. e. 150 to 200 mg/kg). These results differ from those classicaly obtained with other neuroleptics.
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    URL: http://dx.doi.org/10.1007/BF00422970
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  • 31
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    Electronic Resource
    Behavioural and biochemical effects of 6-hydroxydopa in rats (1974)
    Springer
    Psychopharmacology 35 (1974), S. 341-352 
    Details
    ISSN: 1432-2072
    Keywords: 6-Hydroxydopa ; Rat ; Behaviour ; Biogenic Amines
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The influence of 6-hydroxydopa (6-HDP) injected into the lateral ventricles of rat brain on the behaviour of animals was examined. The level of noradrenaline (NA), 5-hydroxytryptamine (5-HT) and 5-hydroxyindoleacetic acid (5-HIAA) in discrete areas of the brain was also measured. 6-HDP was injected in a dose of 100 or 150 Μg into both lateral ventricles, or in a dose of 200 Μg into the right lateral ventricle. Immediately after 6-HDP injection, circling movements, convulsions, aggressive behaviour, and Stereotypic activity were seen. These components of behaviour were most obvious during the first hour after injection of 6-HDP. During one month after 6-HDP administration the behaviour of rats did not differ significantly from the behaviour of control animals, only very subtle differences in behaviour being seen. The dose of 150 Μg of 6-HDP caused aphagia and loss of body weigth during the first 5 days after treatment. 6-HDP also caused hypothermia. 20 min after administration of 200 Μg of 6-HDP a decrease of the NA level but no changes in the 5-HT level in the brain cortex were seen. The same changes were observed 1 month after 6-HDP treatment. The dose of 150 Μg of 6-HDP decreased the NA level in the brain cortex, cerebellum, mesencephalon and brain stem 5 days after treatment. 5-HT content was not changed and the 5-HIAA level was increased in the same brain areas. The dose of 100 Μg of 6-HDP, 2 weeks after the treatment decreased the content of NA in the brain cortex, cerebellum, hypothalamus and brain stem without changes in 5-HT content. 5-HIAA level was elevated only in the brain stem. It is concluded that 6-HDP is a long-acting potent depletor of NA in rat brain. There is a different sensitivity of brain areas to the depleting effect of 6-HDP. 6-HDP does not deplete the 5-HT content of discrete areas of brain but increases the level of 5-HIAA. 6-HDP causes distinct behavioural changes shortly after the treatment. It does not change the behaviour of rats between 1 and 30 days after its administration.
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    URL: http://dx.doi.org/10.1007/BF00429225
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  • 32
    Electronic Resource
    Electronic Resource
    Conditioned aversion in spatial paradigms following methamphetamine injection (1974)
    Springer
    Psychopharmacology 36 (1974), S. 323-335 
    Details
    ISSN: 1432-2072
    Keywords: Methamphetamine ; Rat ; Conditioned ; Aversion ; Space ; Saccharin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Three experiments were performed on Sprague-Dawley derived rats in which 3.0 mg/kg, i.p., injections of methamphetamine were paired with either, (1) a distinctive spatial enclosure, i.e., either a black or gray portion of a shuttle box with discriminably different flooring, (2) the enclosure plus 0.1% saccharin solution availability in the home cage, or (3) the enclosure plus 0.1% saccharin solution availability in that area. Following three drug pairings which alternated with saline injections paired with a different enclosure or enclosure plus H2O availability, a choice of either spatial (1), or spatial plus gustatory stimuli (2,3) which included the original CS's, were presented under non-drug, non-injection conditions. Control groups received saline injections each day under identical conditions. No aversion to the enclosed area, which had been paired with the drug was demonstrated under (1), gustatory (P=0.005) but not spatial aversion was in evidence in the second paradigm, and spatial (P=0.01) and gustatory (P〈0.01) aversion was exhibited in the last study. It was concluded that subsequent aversion to distinctive spatial enclosures following methamphetamine injections was not as strong an effect as similar aversions to gustatory stimuli. Furthermore, the effect only obtained to neutral and not to preferred spatial areas at the dose level tested and in the apparatus used in those studies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00422564
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  • 33
    Electronic Resource
    Electronic Resource
    In vivo studies on uricase activity in the rat (1974)
    Springer
    Pflügers Archiv 351 (1974), S. 323-330 
    Details
    ISSN: 1432-2013
    Keywords: Uricase ; Urate ; Allantoin ; Liver ; Kidney ; Microperfusion ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary 1. In vivo uricase activity was tested in rats by injection of 2-C14 urate and measurement of the total C14 activity and the fractional activities of allantoin, allantoic acid and urea in samples of blood and urine. In control animals, 5 min after the injection, 70% of the plasma tracer was already present in the form of allantoin. No allantoic acid and urea were produced. Intestinectomy had no measurable influence on uricase activity. On the other hand, hepatectomy or ligation of the hepatic artery combined with subtotal viscerectomy did abolish uricase activity almost completely. 2. Following microinjections into proximal tubules of Ringer solution containing 2-C14 urate, urine samples during early recovery mainly contained labelled urate, whereas in later samples the fraction of labelled allantoin increased. About 12 min after the microinjection the urine of both kidneys contained equal amounts of tracer mainly in the form of allantoin. 3. When segments of proximal tubules were perfused with an equilibrium solution containing tracer amounts of C 14 urate, no urate was metabolized during its passage through the proximal tubule. 4. C 14 urate was offered from the peritubular capillaries and samples of tubular fluid were analyzed, Again, all the tracer in the tubular fluid was in the form of urate, indicating that urate is not oxidized when it is transported across the tubular cell. It is concluded from these results that: 1. The rat kidney has no significant uricase activity. 2. Urate transport in the kidney is not influenced by this enzyme. 3. The degradation of urate to allantoin takes place at extrarenal sites, mainly in the liver.
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    URL: http://dx.doi.org/10.1007/BF00593318
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  • 34
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    Electronic Resource
    Cardiac hypertrophy in the first generation of rats native to simulated high altitude (1974)
    Springer
    Pflügers Archiv 350 (1974), S. 241-248 
    Details
    ISSN: 1432-2013
    Keywords: Cardiac Hypertrophy ; Diffusion Distance of Oxygen ; High Altitude ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In rats born and staying their whole life at a simulated altitude of 3500 m a severe cardiac hypertrophy develops which is most pronounced in the right ventricle. On histological sections muscle fiber and capillary densities were counted and the surfaces occupied by muscle fibers and capillaries in the slides were estimated. Mean muscle fiber diameter and half mean distance between capillaries (diffusion distance) were obtained in the heart ventricles and were compared with those of control animals. In both ventricles an increase of the mean muscle fiber diameter occurred (true hypertrophy), being more pronounced in the right ventricle. We cannot tell whether also hyperplasia of muscle fibers occurred or not. The diffusion distance in the left ventricle of rats exposed to hypoxia was about the same as in control animals but was shorter in the right ventricle. This indicates that the total number of capillaries is higher in both ventricles of rats born in the low pressure chamber (hyperplasia of the capillaries). The shortening of the diffusion distance in the right ventricle of rats born and living their whole life at simulated high altitude was on the average smaller than that of rats exposed to simulated high altitude later in life. The physiological importance of this observation is discussed.
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    URL: http://dx.doi.org/10.1007/BF00587803
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  • 35
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    Electronic Resource
    Analysis of periodic components of hypothalamic spike-trains after central thermal stimulation (1974)
    Springer
    Pflügers Archiv 351 (1974), S. 13-24 
    Details
    ISSN: 1432-2013
    Keywords: Hypothalamus ; Spike-Trains ; Correlogram/Periodogram ; Thermosensitivity ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Stationary spike-trains of preoptic neurons of the rat were submitted to a correlation- and spectral-analysis at normal temperature and after central warming and cooling. Correlograms and periodograms were computed which enable the elimination of stochastic signal components and the identification of the relevant periodic components. About one half of the analysed neurons showed periodic correlograms. The following three classes may be distinguished: Neurons which showed correlograms with periodicities at each applied thermal state, at only one or two thermal states and those with no recognizable periodicities at all. Relations between periodicity and the thermosensitivity of the neurons are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00603507
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  • 36
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    Demonstration by degeneration silver method of the ascending projection from the locus ceruleus (1974)
    Springer
    Experimental brain research 20 (1974), S. 181-192 
    Details
    ISSN: 1432-1106
    Keywords: Locus ceruleus ; Cerulo-cortical tract ; Ascending reticular fiber ; Rat ; Fink-Heimer method
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In order to demonstrate the ascending projection from the locus ceruleus by degeneration silver method, the nucleus was destroyed unilaterally in rats and the brains were stained by a modified Fink-Heimer method following various survival times. Additional experiments included the bilateral destruction of the locus ceruleus and the administration of 6-OH-DOPA. For the demonstration of degenerated terminals in the cerebral cortex it is essential to choose an optimal survival time of 4 or 5 days, keep the time of fixation below 1 week, and perform treatments with permanganate and with silver at 37°C. The trajectory and distribution of the cerulo-cortical tract as revealed by the silver degeneration methods is in agreement with the results of the histofluorescence method, with some minor differences. The ascending fibers run through Forel's tegmental fascicle, turn rostro-ventrally to the subthalamus, enter and cross the internal capsule, pass through the ventral part of the caudate-putamen and distribute to the entire cerebral cortex. The tract is mainly ipsilateral, though some fibers are crossing.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00234012
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  • 37
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    Electronic Resource
    Comparative study of the absorption, elimination and acute hepatotoxic action of ethanol in guinea pigs and rats (1974)
    Springer
    Archives of toxicology 32 (1974), S. 83-95 
    Details
    ISSN: 1432-0738
    Keywords: Alcohol, Ethyl ; Absorption ; Elimination ; Alcohol Oxidoreductases ; Hepatitis, Toxic ; Transaminases ; Glutamate Dehydrogenase ; Guinea Pig ; Rat ; Äthylalkohol ; Resorption ; Elimination ; Alkoholdehydrogenase ; Hepatotoxicität ; Transaminasen ; Glutamatdehydrogenase ; Meerschweinchen ; Ratte
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Die maximalen Blutalkoholkonzentrationen nach oraler Gabe von 6,4 g/kg Äthanol in Form einer 40%igen Lösung (v/v) betrugen bei nicht nüchternen Meerschweinchen 6,8±0,3 mg/ml, bei nicht nüchternen Ratten dagegen nur 2,1±0,2 mg/ml. An nüchternen Tieren führte eine orale Alkoholbelastung von 4,8 g/kg zu maximalen Blutalkoholspiegeln von 6,3±0,2 mg/ml bei den Meerschweinchen und 3,7±0,3 mg/ml bei den Ratten. Intravenös injizierter Alkohol (1 g/kg) wurde demgegenüber von beiden Species mit der gleichen Geschwindigkeit eliminiert (275 mg/kg · Std), und die auf das Körpergewicht bezogene ADH-Aktivität der Leber war bei den Meerschweinchen um 20% größer als bei den Ratten. Die Resorption von Äthanol erfolgt somit bei Ratten erheblich langsamer als bei Meerschweinchen. Dies dürfte darauf beruhen, daß konzentrierte Alkohollösungen die Magenentleerung der Ratte stark verzögern. Niedriger konzentrierte Alkohollösungen werden von der Ratte schneller resorbiert; jedoch waren auch nach Gabe von 10%igem Alkohol die maximalen Blutalkoholspiegel bei Ratten noch um 36% niedriger als bei Meerschweinchen. Bei den Meerschweinchen waren nach oraler Gabe von 4,8 bzw. 6,4 g/kg Äthanol die Serumaktivitäten der GOT, GPT und GLDH erhöht; ein Anstieg der SGOT trat schon nach 1,6 g/kg Äthanol auf. Demgegenüber kam es bei Ratten nach 6,4 g pro kg Äthanol p.o. nur zu geringfügigen Aktivitätserhöhungen der Serumtransaminasen, während die Serumaktivität der GLDH unverändert blieb. Vacuolige Degeneration war das morphologische Substrat der durch Äthanol bei Meerschweinchen und Ratten ausgelösten Leberschädigung. Während diese Veränderung bei Meerschweinchen bereits nach einer Äthanoldosis von 1,6 g/kg auftrat, wurde sie bei Ratten erst nach 6,4 g/kg beobachtet. Schlußfolgerung: Das Meerschweinchen ist für Untersuchungen zur Toxicität von Äthanol besser geeignet als die Ratte.
    Notes: Abstract In fed guinea pigs, an oral dose of 6.4 g/kg of ethanol given as a 40% solution (v/v) produced a maximal blood alcohol level of 6.8±0.3 mg/ml, whereas in fed rats, blood alcohol levels after the same dose did not exceed 2.1±0.2 mg/ml. Maximal blood alcohol levels in fasted animals after an oral load of 4.8 g/kg of ethanol were 6.3±0.2 mg/ml in guinea pigs and 3.7±0.3 mg/ml for rats. However, i.v. injected ethanol (1 g/kg) was eliminated at the same rate in both species (275 mg per kg · h), and ADH activity of the liver related to body weight was by 20% greater in guinea pigs than in rats. Therefore, absorption of ethanol occurs at a much slower rate in rats than in guinea pigs. This is possibly due to the fact that high ethanol concentrations strongly delay emptying of the rat stomach. Lowering the ethanol concentration accelerates absorption rate in the rat. However, even after gavage of a 10% solution peak levels of blood alcohol were still lower by 36% in rats than in guinea pigs. In guinea pigs, increased serum activities of GOT, GPT, and GLDH occurred after an oral dose of 4.8 g/kg or 6.4 g/kg of ethanol, respectively. SGOT already increased after 1.6 g/kg of ethanol p.o. After 6.4 g/kg of ethanol given to rats serum transaminase levels increased only slightly, and GLDH activity not at all. Vacuolar degeneration was the morphological substrate of ethanol-induced liver damage in guinea pigs and rats. In guinea-pigs, it occurred already after 1.6 g/kg of ethanol, whereas in rats only after 6.4 g/kg. In conclusion, the guinea pig seems to be better suited for research on alcohol toxicity than the rat.
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    URL: http://dx.doi.org/10.1007/BF00316229
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  • 38
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    Resistance to extinction of an avoidance response in rats following the administration of chlordiazepoxide (Librium) or diazepam (Valium) (1974)
    Springer
    Psychopharmacology 38 (1974), S. 231-238 
    Details
    ISSN: 1432-2072
    Keywords: Rat ; Chlordiazepoxide ; Diazepam ; Extinction ; Avoidance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract In a one-way avoidance task with rats, injections of Librium (chlordiazepoxide) following avoidance acquisition resulted in prolonged resistance-to-extinction of the avoidance response. This effect occurred regardless of whether the rats had had prior experience with Librium or whether they were naive with respect to the drug. The same results were found with the same task when low doses of Valium were used. However, at a higher dosage an “extreme reaction” of either no responding or a high number of responses to extinction occurred in the naive animals. The Librium and Valium effects were compared to similar effects obtained using ethanol and hashish resin. These results indicate that the novelty hypothesis as originally stated by Amit and Baum cannot be supported because experience with the drugs prior to avoidance training did not attenuate the drug effect on avoidance.
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    URL: http://dx.doi.org/10.1007/BF00421375
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  • 39
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    Effect of LSD-25 and 1-methyl-d-lysergic acid butanolamide on rat brain and platelet serotonin levels (1974)
    Springer
    Psychopharmacology 36 (1974), S. 117-122 
    Details
    ISSN: 1432-2072
    Keywords: LSD-25 ; UML ; 5-HT ; Brain ; Platelets ; Behaviour ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Factorial analysis of rat cerebral 5-HT values observed following intraperitoneal LSD-25 and UML (1-methyl-d-lysergic acid butanolamide) showed that the highly significant decrease induced by both drugs was independent of dose and that the effect of both drugs was the same. Increased platelet 5-HT values were also obtained with each drug. These findings suggest that the psychotomimetic effect of LSD-25 is not due to changes in cerebral 5-HT, since a relationship can probably be postulated between such an effect in man and the effect of LSD-25 on conditioned behaviour in the rat. It is also felt that the fact that these drugs decrease and increase cerebral and platelet values respectively may be of assistance in the interpretation of data relating to platelet 5-HT patterns in man under psychiatric conditions and during treatment with psychotropic drugs.
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    URL: http://dx.doi.org/10.1007/BF00421783
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  • 40
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    Effect of acute and chronic ethanol administration on serotonin turnover in rat brain (1974)
    Springer
    Psychopharmacology 37 (1974), S. 91-100 
    Details
    ISSN: 1432-2072
    Keywords: Ethanol ; Brain Serotonin Turnover ; Tolerance ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract In rats treated acutely and chronically with ethanol, brain serotonin (5-HT) turnover was determined by measuring in the same animals the rate of accumulation of 5-HT and the rate of decline of 5-hydroxyindoleacetic acid (5-HIAA) in the brain after administration of the MAO inhibitor pargyline-HCl. In the acute study, 5-HT turnover was not altered after a single 4 g/kg oral dose of ethanol. In two separate studies, it was shown that chronic administration of ethanol in the form of a liquid diet for 32 days also did not alter brain 5-HT turnover, even when the rats had developed a significant degree of tolerance to the motor-impairing effects of ethanol. These results suggest that tolerance to ethanol is unrelated to any change in brain 5-HT turnover.
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    URL: http://dx.doi.org/10.1007/BF00437416
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  • 41
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    Electronic Resource
    Urate handling by the rat kidney (1974)
    Springer
    Pflügers Archiv 352 (1974), S. 115-120 
    Details
    ISSN: 1432-2013
    Keywords: Urate ; Reabsorption ; Loop of Henle ; Micropuncture ; Microperfusion ; Microinjection ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Reabsorption rates for urate in the loops of Henle were measured in superficial nephrons in the rat 1. under conditions of free flow, 2. using microperfusion and 3. by a microinjection technique. 1. Under conditions of free flow distally measured TF/PUA/TF/PIn-values varied between 0.51 and 0.38 in antidiuretic rats, depending on TF/PIn (UA = both uric acid and urate, In = inulin, TF/P = concentration in tubular fluid to plasma concentration). The corresponding values in samples from end-proximal tubules were 1.06 and in urine 0.19 (U/PUA/U/PIn). 2. In microperfusion experiments of Henle loops early distal recoveries of 2-C14 urate varied between 57 and 86%, depending on the flow rates (10–40 nl/min). 3. In microinjection experiments C14 recovery in urine was about 85% when tracer solution was microinjected into endproximal tubules. From these results we conclude: 1. The main site of urate reabsorption is located in the loops of Henle. 2. This reabsorption is highly dependent on flow rates. Increase of flow rate through Henle's loop decreases urate reabsorption.
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    URL: http://dx.doi.org/10.1007/BF00587510
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  • 42
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    Urate interaction with plasma proteins and erythrocytes (1974)
    Springer
    Pflügers Archiv 352 (1974), S. 121-133 
    Details
    ISSN: 1432-2013
    Keywords: Urate ; Protein Interaction ; Uptake by Erythrocytes ; Renal Reabsorption ; Man ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Interaction of urate with human and rat plasma was studied by a dialysis technique at different temperatures. At 4° C a certain fraction of urate is bound to proteins. However, this fraction diminishes with increasing temperature and at 37° C only some 7–8% (in man) and 2% (in rat) interact with proteins. The finding that urate is almost completely filtered in the glomerulus is discussed. In body areas exposed to low temperatures protein binding of urate may be of importance. Urate uptake by erythrocytes is characterized by two components: a fast component equilibrating almost immediately at about 7% in man and 17% in rat and a slow component reaching equilibrium after 60 min, at 28% and 36%, respectively. The process is described by a mathematical formula. Lowering of the temperature mainly diminishes uptake by the slow component withQ 10-values ranging between 1.5 and 4.0. In the observed range of concentrations the uptake process does not saturate. The results at lower pH-values suggest that it is unionized uric acid which is transported by the slow component. Application of the data to kidney medulla supports the hypothesis that erythrocytes and, probably, to a lesser extent plasma proteins serve as vehicles for urate reabsorption in the countercurrent system. Such a temporary interaction enables uric acid to escape recirculation and to leave the kidney medulla.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00587511
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    The effects of scopolamine and physostigmine on fixed-interval behaviour in the rat (1974)
    Springer
    Psychopharmacology 39 (1974), S. 237-244 
    Details
    ISSN: 1432-2072
    Keywords: Antagonism ; Fixed Interval ; Scopolamine ; Physostigmine ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The purpose of this research was to determine if 2 drugs, scopolamine and physostigmine which have opposite effects on cholinergic transmission also produce opposite effects on fixed-interval (FI) performance. Scopolamine and a wide range of doses of physostigmine were administered singly and concurrently to a number of rats responding on a FI 2 min schedule. Scopolamine produced a disruption in FI curvature which could be antagonized by physostigmine, but physostigmine by itself produced no consistent change.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00421031
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  • 44
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    Electronic Resource
    The role of sex and novelty in determining the social response to lithium chloride (1974)
    Springer
    Psychopharmacology 40 (1974), S. 91-99 
    Details
    ISSN: 1432-2072
    Keywords: Lithium ; Social Behaviour ; Sex ; Novelty ; Photographs ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Effects of lithium chloride on the social behaviour of male and female rats were measured in unfamiliar and familiar test situations using a photographic technique. Lithium-treated females showed an increase in body contact and a decrease in social distances in comparison with saline-treated controls in both test conditions. Males administered lithium showed increased corner occupancies in the novel test setting while in the familiar test they showed decreased body contact and increased average group social distance in comparison with the saline condition. Further tests in an exploration box confirmed that lithium decreased the general environmental responsiveness of male rats. The results are discussed with reference to the anti-aggressive effect of lithium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00429450
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  • 45
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    In vitro analysis of the cellular localization of luteinizing hormone releasing factor (LRF) in the basal hypothalamus of the rat (1974)
    Springer
    Cell & tissue research 149 (1974), S. 371-378 
    Details
    ISSN: 1432-0878
    Keywords: LRF ; Hypothalamic localization ; Rat ; Ependyma ; Glia ; Light and electron microscopic Autoradiography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Light and electron microscopic autoradiographic analysis of the medial basal hypothalamus of rat brains incubated for 30 and 60 minutes in tritiated luteinizing releasing factor (3H-LRF) revealed selective uptake in the form of silver grains over specialized ependyma (tanycytes) and perivascular glial cells in the palisade-contact zone of the median eminence. The functional capacity of tanycytes and glia are discussed in light of these data.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00226771
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  • 46
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    Electronic Resource
    Failure to demonstrate serotonergic neurons in the myenteric plexus of the rat (1974)
    Springer
    Cell & tissue research 150 (1974), S. 493-496 
    Details
    ISSN: 1432-0878
    Keywords: Serotonin ; Neurotransmitter ; Myenteric plexus ; Rat ; Histofluorescence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Several recent studies suggested that serotonergic neuron-like elements are present in the guinea pig ileum. The present paper reports an extensive study of the digestive tract of the rat with the use of a histofluorescence technique. Administration of the serotonin precursor, tryptophan, associated with a monoamine oxidase inhibitor, did not allow histochemical demonstration of rapidly fading, yellow fluorescent, 6-hydroxydopamine-resistant neurons; conversely such neurons were readily detected in the brain. It is concluded that serotonergic neuron-like elements cannot be detected histochemically in the rat myenteric plexus area after chemical sympathectomy.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00225972
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  • 47
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    Electronic Resource
    Studies on the juxtaglomerular apparatus (1974)
    Springer
    Cell & tissue research 151 (1974), S. 457-469 
    Details
    ISSN: 1432-0878
    Keywords: Juxtaglomerular apparatus ; Rat ; Adrenalectomy ; Renin and secretory granules ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Die Reaktion des juxtaglomerulären Apparates auf Adrenalektomie wurde untersucht. Im Stadium der Nebenniereninsuffizienz, sechs Tage nach der Operation, wurde quantitativ morphometrisch eine Abnahme der Volumendichte der Sekretgranula (von 32% auf 16%) festgestellt. Golgi-Apparat und Ergastoplasma proliferieren stark, wobei insbesondere die erweiterten Cisternen des Ergastoplasmas auffallen.
    Notes: Summary The response of the Juxtaglomerular apparatus to bilateral adrenalectomy was studied in rats. After the sixth postoperative day, symptoms of severe adrenal insufficiency had developed. In the Juxtaglomerular cells, the volume density of the secretory granules decreased from 32% to 16%. The Golgi apparatus and rough endoplasmic reticulum underwent considerable proliferation, the latter showing strikingly enlarged cisternae.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222991
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  • 48
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    Electronic Resource
    Sex differences in adrenocortical structure and function (1974)
    Springer
    Cell & tissue research 151 (1974), S. 525-536 
    Details
    ISSN: 1432-0878
    Keywords: Adrenal cortex ; Rat ; Sex differences ; Gonadal hormones ; Karyometry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Adrenal glands of adult female rats are heavier than the glands of corresponding male rats. Postpubertal orchiectomy increases the adrenal weight, an effect restored by testosterone replacement. Under the same conditions ovariectomy of 8 weeks duration does not change the adrenal weight while estradiol replacement enhances the relative adrenal weight. Karyometric studies have shown that nuclei in the female zona fasciculata cells are larger (app. 18%) than those of the male. Similar but only slight differences (2%) were observed in the zona reticularis. Orchiectomy results in enlargement of cell nuclei within all zones of the adrenal cortex; testosterone replacement has the opposite effect. Ovariectomy of 8 weeks duration slightly enhances the volume of nuclei of the zona glomerulosa cells, has no effect on the nuclei of the zona fasciculata and reduces the volume of nuclei in the zona reticularis. Estradiol replacement reduces the volume of nuclei of the zona glomerulosa cells but increases nuclear volume in the zona fasciculata and in the zona reticularis. Thus testosterone has an inhibitory effect on the adrenal cortex of the rat while the physiologic effect of estradiol on the morphology of this gland, particularly on the zona fasciculata cells is rather dubious. The author wishes to thank Mrs. B. Westerska and Miss K. Siejak for excellent technical assistance.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222997
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  • 49
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    Electronic Resource
    Sex differences in adrenocortical structure and function (1974)
    Springer
    Cell & tissue research 151 (1974), S. 537-547 
    Details
    ISSN: 1432-0878
    Keywords: Adrenal cortex ; Rat ; Sex differences ; Gonadal hormone ; Stereologic studies
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Adult female rats have heavier adrenals than males. Orchiectomy increases the adrenal weight, an effect reversed by testosterone. Neither ovariectomy nor estradiol replacement has an effect on absolute adrenal weight. The stereologic analysis of the volume fractions of particular adrenocortical zones have shown that the volume fraction of the zona glomerulosa in females is smaller as compared with males. On the contrary, the volume fractions of the fasciculata and reticularis zones are higher in female than in male rats. In both sexes gonadectomy and gonadal hormone replacement has an effect on the volume fractions of particular adrenocortical zones, these changes being more pronounced due to orchiectomy or testosterone replacement. The adrenal cortex of all experimental groups contains a similar quantity of parenchymal cells. The cytoplasm volume of a single adrenocortical cell is higher in all zones of the female adrenals as compared with the male. Orchiectomy increases the volume of the cytoplasm in fasciculata and reticularis cells. This effect was reversed by testosterone. Neither ovariectomy nor estradiol replacement has an effect on the volume of the cytoplasm in the fasciculata and reticularis cells. These studies have shown that sex differences with regards to rat adrenal size and weight depend upon the “physiologic atrophy” of the male adrenal cortex, while neither ovariectomy (8 weeks after operation) nor estradiol replacement has a conspicuous effect on the morphologic changes of this gland.
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    URL: http://dx.doi.org/10.1007/BF00222998
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  • 50
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    Ultrastructural investigations of the palisade-shaped nerve fiber terminals of the normal hairs of rat's snout (1974)
    Springer
    Cell & tissue research 147 (1974), S. 259-269 
    Details
    ISSN: 1432-0878
    Keywords: Nerve endings ; Hair ; Rat ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Palisade-shaped nerve endings of the small normal hairs of the rat snout were examined with the electron microscope (fixation by perfusion). The terminals are located inside the ‘glassy membrane’ in the area of the neck of the hair root. The 10–20 radially arranged terminal axons are in direct contact with the basement membrane of the epithelium of the external root sheath. The axons are surrounded on all sides by leaf-shaped processes of the Schwann cells. The surfaces of these cell processes are marked by numerous vesicle-like invaginations (approx. 1000 Å dia.). Transverse sections from several areas of the palisadeshaped nerve endings are compared with longitudinal sections. In the upper area ‘empty’ vesicles (approx. 500–600 Å in diameter) occur, along with electron-dense vesicles (approx. 800–1100 Å in diameter); in the middle area, the axons are distended and contain accumulations of mitochondria.
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    URL: http://dx.doi.org/10.1007/BF00582800
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    The parietal layer of bowman's capsule in experimental hypertension (1974)
    Springer
    Cell & tissue research 147 (1974), S. 183-190 
    Details
    ISSN: 1432-0878
    Keywords: Kidney ; Rat ; Bowman's capsule ; Experimental hypertension
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The parietal layer of Bowman's capsule was examined in the rat in control animals and in animals made hypertensive by treatment with desoxycorticosterone acetatesaline and by clipping of the renal artery. At an early stage of elevation of blood pressure and, occasionally, prior to elevation of blood pressure, the epithelial cells of this layer showed basal cytoplasmic protrusion and reduplication accompanied by an increase in the thickness of the basement membrane. These changes preceded evidence of glomerular or renal arteriolar pathology. They presumably reflect either a response of this layer to increased blood pressure and modified glomerular function, or a direct effect of the factors that also induces blood pressure elevation. In either case, they imply a greater functional significance for the parietal layer than has usually been attributed to it.
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    URL: http://dx.doi.org/10.1007/BF00582793
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  • 52
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    The ultrastructure of the accessory sex organs of the male rat (1974)
    Springer
    Cell & tissue research 148 (1974), S. 57-67 
    Details
    ISSN: 1432-0878
    Keywords: Prostate ; Rat ; Cyproterone ; Alterations ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The dorsal lobe and the coagulating gland of the rat prostate were studied after administration of the anti-androgenic compound Cyp A for varying periods (7–20 days). There was a general involution of the organs with macroscopic atrophy and reduced amount of secretory material. Ultrastructurally the cells were pale, contained less organelles as compared with the controls, loss of cytoplasm and reduction both of cell height and width. The major changes also included reduction of the Golgi area, the rough endoplasmic reticulum and the number of free ribosomes. Within the dorsal lobe prominent nuclear changes were found, comprising different types of intranuclear inclusions of membranous configurations. The alterations observed in the present study are similar to the changes which were found in castrated animals and are consistent with atrophy. The results are discussed in relation to the available biochemical and autoradiographic data on the different lobes.
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    URL: http://dx.doi.org/10.1007/BF00224318
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  • 53
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    Electronic Resource
    Ultrastructure of mucosal mast cells in normal and compound 48/80-treated rats (1974)
    Springer
    Cell & tissue research 150 (1974), S. 95-105 
    Details
    ISSN: 1432-0878
    Keywords: Mucosal mast cells ; Rat ; Compound 48/80 ; Degranulation, Release ; Light microscopy ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Mast cells in the tongue and lamina propria of the duodenal mucosa, representing two cell types with different morphological, histochemical and functional properties have been studied under the electron microscope. Both cell types were found to contain similar moderately electron dense and homogeneous granules surrounded by a tight membrane constituting the basic ultrastructural characteristics of mast cells. The mucosal mast cells, however, contained fewer granules per cell and the individual granules often exhibited considerable variation in size. In addition the mucosal mast cells, unlike those of the tongue, peritoneum and skin, had a smooth plasma membrane lacking microvilli and possessed irregularly shaped or lobulated nuclei. In the tongue mast cells, administration of Compound 48/80 caused the formation of vacuoles around the granules, expulsion of granules from the cells and disintegration of the granular matrix, but no changes that could be attributed to 48/80 could be found in the mucosal mast cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00220383
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  • 54
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    Kinetics of gametogenesis (1974)
    Springer
    Cell & tissue research 154 (1974), S. 443-470 
    Details
    ISSN: 1432-0878
    Keywords: Oocytes and T-prospermatogonia ; Rat ; Quantitative analysis ; Autoradiography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Bei der Ratte findet die sexuelle Differenzierung der Gonade zwischen dem 14. und 15. Tag post conceptionem (p.c.) statt. Während dieser Zeit teilen sich die Oogonien und deren Parallelpopulation—die M-prospermatogonien (multiplying prospermatogonia) oder I-Gonocyten—sehr häufig. Um den 17. Tag p.c. tritt die letzte Generation der Oogonien bzw. der M-prospermatogonien in die Mitose. Die Mitosen bilden häufig “synchronisierte Gruppen”. Die postmitotischen Kerne ihrer Tochterzellen—der Oocyten und der T 1 prospermatogonien (primary transitional prospermatogonia) oder II-Gonocyten—sind klein. Ihr Chromatin ist in Form gröberer Schollen der Kernmembran angelagert. Auf diese Weise kommt das typische “krustenförmige” Aussehen bzw. die Ähnlichkeit mit den Prophasen der Oogonien und M-prospermatogonien zustande. Die Oocyten durchlaufen zunächst die G1-phase (etwa 10 Std Dauer) und treten dann—am Ende des Präleptotänstadiums—in die S-phase. Dann passieren sie die verschiedenen Stadien der meiotischen Prophase und treten vom 3. Tag post partum (p.p.) ab in das Dictyotänstadium. Die T1-prospermatogonien hingegen befinden sich etwa 10 Tage lang in der G1-phase, ohne wesentliche morphologische Veränderungen aufzuweisen. Vom 4. Tag p.p. an durchlaufen sie die S-Phase. Die S-Phasen-Dauer (D-S) beider Zellarten beträgt 11.5 Std und wurde durch Doppelmarkierung mit 14C- und 3H-Thymidin bestimmt. Als am besten geeignete Termine für die Bestimmung der D-S erwiesen sich bei den Oocyten der 18. Tag p.c. und bei den T1-prospermatogonien der 5. Tag p.p. Zu diesen beiden Zeitpunkten war die Anzahl der in die S-phase ein-und austretenden Oocyten bzw. T1-prospermatogonien gleich, die Zellen in S-phase befanden sich im “steady state”. Das Kernvolumen der Oogonien und M-prospermatogonien ist etwa doppelt so groß wie das der postmitotischen Oocyten und T1-prospermatogonien. Bis zum 5. Tage p.p. nimmt das Kernvolumen der Oocyten und T1-prospermatogonien um etwa das Fünffache zu. Der Degenerationsindex der Oocyten liegt wesentlich höher als der der T1-prospermatogonien; er ist postnatal besonders hoch. Aus den T1-prospermatogonien gehen am 4. und 5. Tage p.p. durch Teilung die T 2-prospermatogonien (secondary transitional prospermatogonia) hervor. Die Kerne dieses Zelltyps sind etwas kleiner also die der T1-prospermatogonien. Die T2-prospermatogonien treten am 6. Tage p.p. in die Mitose; es entstehen die ersten A-spermatogonien.
    Notes: Summary In the rat (Wistar-WU) sexual differentiation of the gonads occurs between days 14 and 15 post conception (p.c.). At this time the oogonia and their parallel population — the M-prospermatogonia (I-gonocytes)—divide rapidly. On about day 17 p.c., the last generation of oogonia and M-prospermatogonia, frequently arranged in synchronized clusters, enters mitosis. The postmitotic nuclei of their daughter cells—oocytes and T 1-prospermatogonia (II-gonocytes)—are small; coarse flakes of chromatin are associated with the nuclear membrane causing the typical “crustlike” appearance and the similarity with the prophases of oogonia and M-prospermatogonia. After the oocytes have passed a G1-phase of approximately 10 hr, they enter the S-phase at the end of the preleptotene stage. Then they pass the different stages of the meiotic prophase until they enter the dictyate stage from 3 day post partum (p.p.) onwards. The T1-prospermatogonia, on the other hand, spend a long G1-phase of about 10 days without any conspicuous morphological change before entering the S-phase from day 4 p.p. onwards. The duration of the S-pbase (D-S) of both cell types—oocytes and T1-prospermatogonia—as determined by the double labeling method with 14C- and 3H-thymidine is found to be 11.5 hr. The most favourable time for determining the D-S was day 18 p.c. for the oocytes and day 5 p.p. for the T1-prospermatogonia. On these two days the balance was reached between the cells entering and leaving the S-phase. The nuclear volumes of the postmitotic oocytes and T1-prospermatogonia are approximately half the size of those of their precursors. Until day 5 p.p. the nuclear volumes of the oocytes and T1-prospermatogonia increase about fivefold. The degeneration index of the oocytes is considerably higher than that of the T1-prospermatogonia; postnatally it is especially high. T 2 prospermatogonia arise by mitosis of the T1-prospermatogonia on day 4 and 5 p.p. The nuclei of this cell type are smaller than those of T1-prospermatogonia. T2-prospermatogonia enter mitosis on day 6 p.p and give rise to A-spermatogonia.
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    URL: http://dx.doi.org/10.1007/BF00219667
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    Development of serotonin-containing cells and the sympathetic innervation of the habenular region in the rat brain (1974)
    Springer
    Cell & tissue research 155 (1974), S. 231-243 
    Details
    ISSN: 1432-0878
    Keywords: Pinealocyte-resembling cells ; Habenular region ; Sympathetic nerve fibres ; Ontogenetic development ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The cells of the pineal gland, the pineal stalk, and the lamina intercalaris contain 5-HT and are innervated by sympathetic nerve fibres. These peripheral nerve fibres continue rostrally from the lamina intercalaris and run into the central nervous tissue of stria medullaris and the habenular nuclei. Pharmacological treatment to increase the cellular 5-HT content revealed that the sympathetic fibres are in close relation to yellow fluorescent cells embedded in the brain tissue. These yellow fluorescent cells develop very late in the ontogenetic development (three weeks or more postnatally) and are preceded by ingrowth of sympathetic fibres into the brain tissue. The results support the hypothesis that the cells found in the habenular region are of pinealocyte rather than neuronal nature, but it is possible that they differ in certain aspects from the cells of the pineal gland proper.
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    URL: http://dx.doi.org/10.1007/BF00221357
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  • 56
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    Electronic Resource
    Somatic spines in the supraoptic nucleus of the rat hypothalamus (1974)
    Springer
    Cell & tissue research 148 (1974), S. 203-211 
    Details
    ISSN: 1432-0878
    Keywords: Hypothalamus ; Rat ; Supraoptic nucleus ; Ultrastructure ; Somatic spines
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Electron microscopic studies of ultrathin serial sections of the perikarya and adjacent neuropil of neurons of the supraoptic nucleus (SON) of the adult male rat revealed varying forms of two types of somatic spines. One type forms synapses with axons passing the cell, the other, without synapses, appears to serve as a buttress or clasp for adjacent neuronal and glial processes. The synapse-bearing spines lack the usual spine apparatus but contain the flocculent substance often seen in spines. The other spines do not exhibit either of these structures. These somatic spines were also seen in Golgi impregnated preparations but the types could not be distinguished. Certain axons synapse either on a somatic spine of the perikarya or penetrate the glial sheath of the neuron and synapse, usually repeatedly, on the soma in an en passant manner.
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    URL: http://dx.doi.org/10.1007/BF00224582
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  • 57
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    Ultrastructure of rat pineal gland after preparation by Freeze-etching technique (1974)
    Springer
    Cell & tissue research 148 (1974), S. 371-379 
    Details
    ISSN: 1432-0878
    Keywords: Pineal gland ; Rat ; Electric coupling areas ; Freeze-etching
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The pineal glands of Wistar rats were fixed in glutaraldehyde and prepared by the freeze-etching technique. With regard to the form and internal structure of pineal cells this technique has revealed nothing essentially new. Synaptic ribbons are very rarely found. A regular diagonal arrangement of membrane attached particles between the Schwann cell and the axon of an autonomic nerve terminal was observed. Three types of contact areas between pineal cells are described. In the first two types, which occur more frequently, the diameter of the area varied from 1000 to 2500 Å, with irregularly aggregated membrane associated particles. The third type, larger but less frequently found, has a surface area of 0.4 μm2 and much more regularly distributed membrane associated particles (ø 88 Å). These contact areas can be considered to be electric coupling areas between pineal cells.
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    URL: http://dx.doi.org/10.1007/BF00224264
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  • 58
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    Electronic Resource
    Indoleamine-containing nerve terminals in the rat median eminence (1974)
    Springer
    Cell & tissue research 147 (1974), S. 285-292 
    Details
    ISSN: 1432-0878
    Keywords: Median eminence ; Rat ; Serotonin terminals ; 5,6- and 5,7-Dihydroxytryptamine ; Chemical degeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Electron microscopical evidence for the existence of an important serotonergic input to the rat median eminence is presented. This evidence is based on the demonstration of degenerating nerve terminals in the external layer of the rat median eminence following the application of 5,6-dihydroxytryptamine or 5,7-dihydroxytryptamine, drugs known to exert more or less selective toxic effects on central serotonin neurons. The finding of a serotonergic innervation of the median eminence in mammals has important implications for 5-hydroxytryptamine as a modulator of synthesis and/or release of hypothalamic, hypophysiotropic hormones.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00582802
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  • 59
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    Electronic Resource
    On the ultrastructure of the rat anococcygeus muscle (1974)
    Springer
    Cell & tissue research 149 (1974), S. 91-104 
    Details
    ISSN: 1432-0878
    Keywords: Anococcygeal muscle ; Smooth muscle ; Rat ; Innervation, adrenergic
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The rat anococcygeus muscle, which is known from previous functional and histochemical investigations to be a smooth muscle with a dense adrenergic innervation, was studied by electron microscopic methods. The muscle was found to be organized in small bundles of 2 to 8 muscle cells, which were interconnected by desmosome-like junctions and by gap junctions. Small Schwann cell-axon complexes with 1 to 5 axons appeared to represent the functional terminal nerve pathway. Such axon complexes were located at the periphery of the small smooth muscle bundles, they never penetrated into them. A ratio of approximately 6 small axon complexes per 100 muscle cells was found. After glutaraldehyde fixation axon varicosities contained mainly small empty vesicles. After treatment with 5-hydroxydopamine and permanganate fixation mainly small vesicles with a dense core were found. The neuromuscular distances ranged from 550 to 5000 Å, with a mean of about 2600 Å. True close neuromuscular contacts at a distance below 500 Å with a fusing of basal laminae were absent. The present observations provide a morphological base for the findings of previous electrophysiological and pharmacological studies on the anococcygeal muscle.
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    URL: http://dx.doi.org/10.1007/BF00209052
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  • 60
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    Ultramorphometric studies regarding the effect of sex-hormones on the adrenal cortex of the rat (1974)
    Springer
    Cell & tissue research 149 (1974), S. 113-120 
    Details
    ISSN: 1432-0878
    Keywords: Adrenal cortex ; Rat ; Sex-hormones ; Ultramorphometry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Ultramorphometrisch wurde nach dem Verfahren von Rohr et al. (1971) die äußere Zona fasciculata von Sprague-Dawley-Ratten bei 5 Gruppen zu je 4 Tieren verglichen: Männchen, Weibchen im Diöstrus, Weibchen im Östrus, männliche und weibliche Kastraten (Kastration am 4. Lebenstag). Die Nebennierenrindenzellen der äu\eren Fasciculata sind bei Weibchen gegenüber denen normaler Männchen hypertroph: Weibchen im Diöstrus zeigen gegenüber Männchen größere Einzelzellen mit vermehrtem glattem endoplasmatischem Reticulum, erhöhten Einzel- und Gesamtvolumina der Mitochondrien sowie einem höheren Lipoidgehalt bei stärkerer Dispersion der Liposomen. Dieselben funktionsspezifischen Strukturen nehmen bei den Weibchen im Östrus noch weiter zu. Die Kastration der Weibchen am 4. Lebenstag führt zu einer Reduktion der funktionsspezifischen morphometrischen Parameter unter die Werte der Diöstrus-Tiere. Männliche Kastraten gleichen die Werte ihrer funktionsspezifischen Strukturen weitgehend denen der Weibchen im Diöstrus an. Nach Kastration am 4. Lebenstag zeigen weibliche Tiere somit etwas niedrigere morphonietrische Parameter in der äußeren Zona fasciculata als die männlichen Kastraten.
    Notes: Summary With the method of Rohr et al. (1971) the outer zona fasciculata of Sprague-Dawley-rats was compared ultramorphometrically in 5 groups of 4 animals each: males, females during diestrus, females during estrus, male and female castrates (castration on 4th day of life). Adrenal cortical cells of the outer fasciculata in females are hypertrophic in comparison with those of normal males: females during diestrus show in comparison with males larger individual cells with increased SER, increased volume of the individual mitochondrium and total volume of mitochondria as well as an increased lipoid content together with a more pronounced dispersion of liposomes. The same function-specific structures show a further increase in females during estrus. Castration of females on the 4th day of life leads to a reduction of the function-specific morphometric parameters below the values of the diestrus animals. Male castrates show adaption of the values of their function-specific structures towards those of females during diestrus. Following castration on the 4th day of life, female animals thus show somewhat lower morphometric parameters in the outer zona fasciculata than male castrates.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00209054
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    The fine structure of adrenal zona glomerulosa in the adult rat (1974)
    Springer
    Cell & tissue research 149 (1974), S. 235-243 
    Details
    ISSN: 1432-0878
    Keywords: Adrenal zona glomerulosa ; Rat ; Normal ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary This paper reports on an electron microscopic study of the adrenal zona glomerulosa in the normal rat, with special emphasis on the structural diversity of mitochondria in its parenchymatous cells. The mitochondria of cristae may be tubulo-vesicular, plate-like and/or straight tubular, any one mitochondrion containing one or more types of cristae. The mitochondrial features of the zona glomerulosa parenchymatous cells are attributed to the biochemical stages of aldosterone synthesis, taking place in different mitochondria of these cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222276
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  • 62
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    Different populations of granules and their distribution in the hypothalamo-neurohypophysial tract of the rat under various experimental conditions (1974)
    Springer
    Cell & tissue research 151 (1974), S. 117-140 
    Details
    ISSN: 1432-0878
    Keywords: Hypothalamo-neurohypophysial system ; Rat ; Granule distribution ; Electron microscopy, morphometry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Die Neurohypophyse und die neurosekretorischen hypothalamischen Kerngebiete von normalen männlichen und weiblichen Ratten, von trächtigen und durstenden Tieren wurden elektronenmikroskopisch und morphometrisch mit der Fragestellung untersucht, ob sich in den Häufigkeitsverteilungen der mittleren Granula-Durchmesser, bezogen auf die Anzahl der Axone, eine zweigipflige Verteilung ergibt. Bei Kontrolltieren zeigen die Perikarya des Nucleus supraopticus zahlreichere und größere Granula als die Zellen des Nucleus paraventricularis, wobei der Unterschied in den Durchmessern der Granula bei den weiblichen Tieren ausgeprägter ist als bei den männlichen. Beide Kontrollgruppen zeigen in der Neurohypophyse ein zweigipfliges Verteilungsmuster der Granula, wobei die beiden Gipfel bei den männlichen Kontrollen dichter beieinander liegen als bei den weiblichen. Bei den trächtigen Tieren weisen beide Kerngebiete Zeichen gesteigerter Aktivität auf und die Granula in den Zellen des Nucleus paraventricularis sind deutlich größer als bei Kontrolltieren. Zu Beginn der Tragzeit werden relativ große, wahrscheinlich vasopressinhaltige Granula in der Neurohypophyse gespeichert, während sich im letzten Drittel der Tragezeit die beiden Populationen dort nicht mehr unterscheiden lassen. Nach 48 Std. Wasserentzug erscheinen beide hypothalamischen Kerne aktiviert. Auffallend ist, daß die Granula im Nucleus supraopticus deutlich kleiner sind als bei Kontrolltieren. Entsprechend fehlen in der Neurohypophyse große Granula, während Axone mit kleinen Granula vermehrt sind. Nach zehntägiger Durstperiode lassen sich im Nucleus supraopticus, neben sehr typischen morphologischen Veränderungen, morphometrisch ebenfalls deutlich kleinere Granula nachweisen. Im Hypophysenhinterlappen dieser Versuchsgruppe sind die granulahaltigen Axone sehr stark vermindert, und in der Häufigkeitsverteilung fehlen die Granula mit großem Durchmesser während der dem Oxytocin zugeordnete Gipfel unverändert erscheint. In Verbindung mit den elektronenmikroskopischen Befunden lassen sich also aus den Verschiebungen der Verteilungsmuster zwischen den sechs Versuchsgruppen die Granula mit kleinerem Durchmesser oxytocinhaltigen Axonen zuordnen und Granula mit größerem Durchmesser zu den Endigungen, die Vasopressin enthalten. Die morphometrischen Befunde in den neurosekretorischen hypothalamischen Kerngebieten bestätigen diese Zuordnung. Die elektronenmikroskopischen und morphometrischen Befunde werden besonders im Hinblick auf die Hormonverschiebungen zwischen dem “reserve pool” und den “easily releasable pool” diskutiert.
    Notes: Summary A morphometric evaluation of electron micrographs has been carried out from neurosecretory terminals in the neurohypophysis and from the supraoptic and paraventricular nuclei in normal male and female rats as well as in pregnant and water deprived rats. The task of this investigation was to find out whether frequency distribution diagrams of the mean diameter of the neurosecretory granules, plotted versus the number of axons, reveal a grossly bimodal distribution. In normal rats the nerve cells of the nucleus supraopticus (S.O.N.) show more numerous and larger granules than the cells of the nucleus paraventricularis (P.V.N), this difference with respect to the diameter being more pronounced in female than in male animals. In the posterior pituitary lobes the neurosecretory granules exhibit a bimodal distribution, the second peak being situated more closely to the first one in male controls. In pregnant animals both nuclei appear to be activated, and the granules of P.V.N. are distinctly larger as compared to those of female control animals. In early pregnancy larger, i.e. presumably vasopressin-containing granules are stored in the neurohypophysis, while in the last phase before delivery oxytocin-containing neurons and vasopressin-containing ones cannot be differentiated any more. After 48 hrs of thirst both hypothalamic nuclei exhibit signs of increased activation, the diameters of the granules in the S.O.N. being distinctly smaller than in normals. In the neurohypophysis granules with large diameters are lacking, while axons with small granules are more frequent. In the S.O.N. of animals, water deprived for 10 days the diameters of the granules are smaller than normal and in the neurohypophysis the number of granule-containing fibres is strongly reduced. In the frequency distribution diagram granules with larger diameters are lacking whereas the peak, attributed to oxytocin, is not essentially altered. However, from the observed alteration on the distribution patterns of the six experimental groups and in connection with the electron microscopic results the granules with smaller diameters can be attributed to oxytocin-containing axons and the larger ones to vasopressin-containing terminals. Analogous morphometric data have been obtained from the neurosecretory nuclei as well. The electron-microscopic and morphometric observations are discussed with regard to hormone-shifts between the reserve pool and the easily-releasable pool.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222039
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    Studies on the juxtaglomerular apparatus (1974)
    Springer
    Cell & tissue research 151 (1974), S. 471-480 
    Details
    ISSN: 1432-0878
    Keywords: Juxtaglomerular apparatus ; Rat ; Influence of deoxycorticosterone (DOC) ; Electron microscopy ; Morphometric analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Die quantitativ-morphologische Analyse des juxtaglomerulären Apparates nach DOC-Behandlung zeigte, daß mit der Hemmung der Reninbildung eine Hypoplasie der epitheloiden Zellen des juxtaglomerulären Apparates einhergeht. Der Granulagehalt der juxtaglomerulären Zellen nahm ab; die Kern-Plasma-Relation änderte sich und die Leiomyofibrillen in den Epitheloidzellen nahmen zu. An den übrigen Anteilen des juxtaglomerulären Apparates, insbesondere der Macula densa, wurden keine morphologischen Veränderungen beobachtet. Die DOC-Behandlung führte darüber hinaus zu einer Bildung von Einschlüssen in den Podocyten, den Mesangiumzellen und den Zellen der Bowman-Membran. Die verschiedenen Möglichkeiten, über welche Faktoren die juxtaglomerulären Zellen nach DOC-Behandlung degranuliert werden, werden diskutiert. Das ultrastrukturelle Bild der Epitheloidzellen nach DOC-Behandlung entspricht deutlich einer gehemmten Sekretion, da das Ergastoplasma und der Golgi-Apparat ganz besonders stark zurückgebildet werden.
    Notes: Summary Quantitative morphological analysis of the Juxtaglomerular apparatus of rats treated with deoxycorticosterone (DOC) and salt revealed hypoplasia of the epithelioid cells as a concomitant of the inhibition of renin synthesis. The granular content of the Juxtaglomerular cells was significantly decreased, while the nucleus-to-cytoplasm ratio and the volume density of leiomyofilaments in the epithelioid cells increased. No morphological alterations were observed in the remaining constituents of the Juxtaglomerular apparatus, including the macula densa. DOC-treatment also induced the appearance of inclusions in the podocytes in the cells of the mesangium, and in the cells of Bowman's capsule. The possible mechanisms of degranulation of the Juxtaglomerular cells after treatment with DOC are discussed. The ultrastructural appearance of the epithelioid cells after treatment with DOC corresponds to an inhibition of secretion, since the rough endoplasmic reticulum and the Golgi apparatus undergo marked regressive changes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222992
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    Einfluß der Splenektomie auf Magensaftsekretion und Ulcusentstehung der Ratte (1974)
    Springer
    Langenbeck's archives of surgery 336 (1974), S. 15-23 
    Details
    ISSN: 1435-2451
    Keywords: Rat ; Splenectomy ; Gastric Ulceration ; Increase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung An Ratten wurde der Einfluß von Splenektomie auf Magensaftsekretion und Ulcusentstehung untersucht. Sowohl nach “restraint-stress” als auch Pylorusligatur wiesen die splenektomierten Tiere eine ausgeprägte Steigerung der Ulcuszahl und -größe gegenüber den Kontrolltieren auf. In der Magensaftanalyse waren dagegen die Werte der splenektomierten Tiere gegenüber den scheinoperierten nur geringgradig gesteigert. — Als mögliche Ursache der vermehrten Ulcusanfälligkeit nach Splenektomie wurde eine Veränderung lokaler, nicht säuresekretorischer Faktoren der Magenwand diskutiert.
    Notes: Summary In Splenectomiced rats incidence of gastric ulceration was compaired with control groups. Restraint stress and ligation of pylorus was used as model. Splenectomiced rats do show an higher incidence in both models. Both frequency and extension of gastric ulcer were accumulated significantly. Analysis of gastric secretion was only unessentially raised in comparisom to control-groups. Change of protective factors of stomach as possible reason is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01291870
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    Chondrogenesis in the external ear of the rat (1970)
    Springer
    Anatomy and embryology 131 (1970), S. 236-242 
    Details
    ISSN: 1432-0568
    Keywords: Cartilage ; Ear, external ; Differentiation ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The differentiation of cartilage in the external ear of the rat proceeds in four steps. Each of them is characterized by a specific biosynthetic activity of the chondroblast resulting in the appearance of a new structural component of the tissue: collagen fibrils, elastin fibers, cartilage ground substance and intracellular fat droplets. As a structurally and developmentally well-defined “subspecies” of cartilage, this tissue might serve as a suitable object for the experimental approach to some essential problems in histodifferentiation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00520966
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    Elektronenmikroskopische Untersuchungen zum Problem des Stoffaustausches zwischen Mutter und Keim bei Rattenembryonen des Tages 7–10 (1970)
    Springer
    Anatomy and embryology 131 (1970), S. 325-346 
    Details
    ISSN: 1432-0568
    Keywords: Rat ; Early development ; Jolk-sac entoderm ; Reichert's membrane ; Trophoblast-cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Rattenkeime des Schwangerschaftstages 7–10 wurden elektronenmikroskopisch untersucht. Das viscerale einschichtige Dottersackentoderm zeichnet sich durch eine ausgeprägte endocytotische Aktivität aus. Die Zellen des parietalen Blattes dagegen liegen isoliert der Reichertschen Membran innen an und enthalten vorwiegend rauhes endoplasmatisches Reticulum. Die Reichertsche Membran besteht aus einem lockeren Maschengitter feiner Filamente. Im antimesometrialen und lateralen Bereich haften nur wenige Trophoblastenzellen dieser Membran von außen an, dazwischen befinden sich weite Lücken. Zum mütterlichen Gewebe hin wird der Keim mit Ausnahme des mesometrialen Poles von einem bis zu 50 μ breiten periembryonalen Sinus umgeben, der mütterliches Blut enthält. Nach außen wird dieser Raum durch Trophoblasten- und Deciduazellen abgedichtet. Aus diesen Befunden wird geschlossen: 1. Die Reichertsche Membran wird vom parietalen Dottersack-Entoderm gebildet. 2. Mütterliches Blutplasma kann frei das viscerale Dottersack-Entoderm erreichen, da als einzige kontinuierliche Schicht zwischen Blut und Träger nur die weitmaschige Reichertsche Membran vorhanden ist. 3. Durch das endocytotische und lysosomale System des visceralen Dottersackentoderms wird das anströmende mütterliche Plasma aufgenommen, abgebaut und für den wachsenden Keim nutzbar gemacht. 4. Immunbiologisch betrachtet können Antikörper der Mutter den Keim erreichen. 5. Embryonale Antigene dagegen erreichen durch die Errichtung einer Deciduabarriere nicht den mütterlichen Kreislauf. 6. Der periembryonale Sinus stellt einen Blindsack dar, in den zwar mütterliches Blut einströmen kann, ein Ausstrom aber nicht mehr möglich ist.
    Notes: Summary Electron microscope studies of rat embryos were performed from the 7th to 10th days of pregnancy. The single-layered visceral yolk entoderm is characterized by a marked endocytotic activity. The cells of the parietal layer lie separately adjacent to the Reichert's membrane and they contain mainly rough endoplasmic reticulum. The Reichert's membrane consists of a loose network of fine filaments. In the antimesometrial and lateral sites only few trophoblast cells are seen to be attached to the membrane. Between them we find large gaps. With the exception of the mesometrial pole, the embryo is surrounded by a periembryonal sinus, 10–50 μ wide, which contains maternal blood. This sinus space is sealed tightly (zonulae occludentes) by trophoblast and decidual cells. From these findings it was concluded: 1. Reichert's membrane is formed from the parietal jolk sac entoderm. 2. The porous Reichert's membrane permits the maternal plasma to reach freely the visceral jolk sac entoderm. This membrane is the only continuous layer between blood and egg cylinder. 3. By the endocytotic and lysosomal system, the visceral jolk sac entoderm absorbs the plasma coming from the mother. This plasma is degraded and used for the growing embryo. 4. From the immunobiological point of view the antibodies from the mother can reach the embryo freely, 5. The embryonal antigens cannot reach the maternal circulation because of the presence of a decidual barrier, 6. The perimebryonal sinuses are blind sacs into which the maternal blood can flood but it cannot return.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00519974
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    Über Faltungen der Kernmembranen (Kernschrumpfung) des Ependyms im III. Ventrikel der Ratte nach langdauernder intraperitonealer Narkose mit Natrium-5-(β-Methylthioäthyl)-5-(2′-pentyl)-thiobarbitursäure („Thiogenal”) (1970)
    Springer
    Acta neuropathologica 16 (1970), S. 44-53 
    Details
    ISSN: 1432-0533
    Keywords: Ependyma ; Thiobarbiturate ; Rat ; Hypothalamus ; III. Ventricle ; Narcosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung An 14 männlichen Ratten wurde nach langdauernder intraperitonealer Narkose mit Thiobarbiturat Veränderungen des Ependyms im III. Ventrikel festgestellt. In der Gegend über dem Nucleus ventromedialis und Nucleus dorsomedialis hypothalami lassen sich zwei Formen des Ependyms unterschieden: 1. ein einschichtiges hohes Ependym mit runden großen Zellkernen, 2. ein mehrreihiges flaches Ependym mit schmalen, ovalen Zellkernen. Das flache Ependym zeigt folgende Veränderungen: Farbdichte und die Menge des färbbaren Materials im Kern nehmen zu, die Kernmembran ist gefaltet, der Kern zeigt Zeichen der Schrumpfung und Pyknose. Die Kontrollgruppe wurde mit Curare behandelt. Eine statistische Sicherung erbrachte einen signifikanten Unterschied mit einer Irrtumswahrscheinlichkeit von 0,5‰.
    Notes: Summary Alterations in the ependyma of the third ventricle were caused by continuous intraperitoneal thiobarbiturate narcosis in14 male rats. In the region of the nucleus dorsomedialis and ventromedialis hypothalami two kinds of ependyma can be distinguished, 1. a simple cuboidal ependyma with a large rounded nucleus, and 2. a stratified squamous ependyma with a slender oval nucleus. The squamous ependyma shows the following changes. The depth of staining and the chromatin material of the nucleus increases, the membrane of the nucleus is folded, the nucleus shows the signs of shrinkage and pyknosos. The control group was treatened with Curare. The significant difference of the results encloses 0.5‰ probability of error.
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    URL: http://dx.doi.org/10.1007/BF00686963
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    Acoustically evoked potentials in the rat during sleep and waking (1970)
    Springer
    Experimental brain research 11 (1970), S. 93-110 
    Details
    ISSN: 1432-1106
    Keywords: Auditory evoked potentials ; Sleep ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Click-evoked potentials were recorded from the rat's auditory cortex (AC), medial geniculate body (MGB), reticular formation (RF), and hippocampus (HIPP) during natural sleep and waking. Various amplitude measurements made on sliding averages by a computer provided essentially continuous measurement of evoked responses in long experiments. Changes in AC responses were simplest in potentials recorded from a depth of 1.0–1.5 mm, all components of the average waveform being larger during slow-wave sleep (SS) than in waking (W) and low-voltage fast sleep (LVFS). More complex changes in cortical surface responses included increases in the first positive wave, in a second positive deflection, and in a late negative wave during drowsiness (D) and SS. The second positive wave remained large during LVFS, while the late negativity changed form and peaked earlier. Early components of both surface and deep responses were not consistently different in W and LVFS. The only clear change in MGB responses was a reduction in amplitude during LVFS. Late positive waves in RF and HIPP responses were large during SS and small during W and LVFS. In general, increases in the very late components of responses at all sites distinguished periods of cortical synchrony from periods of cortical activation. The first negative deflection and a succeeding positive wave in the HIPP response were markedly increased during arousal.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00234203
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    The identification of fractions enriched in nonmyelinated axons from rat whole brain (1970)
    Springer
    Experimental brain research 11 (1970), S. 392-410 
    Details
    ISSN: 1432-1106
    Keywords: Subcellular fractionation ; Axons ; Synaptosomes ; Whole brain ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Subfractionation of a standard “synaptosome” fraction from whole brain has yielded two lighter layers rich in axonal segments devoid of synaptosomes similar to those obtained from cerebellar homogenates. The heavier layers were found to contain both synaptosomes and axonal segments plus a significant population of synaptosomes with axons attached. Quantitative studies of the percent perimeter of external membrane contributed by the various elements within the subfractions showed that the major constituents in decreasing order were axonal segments, synaptosomes, membranes of undetermined origin and free mitochondria. These findings have revealed that “synaptosome” preparations are more heterogeneous than previously reported and at the same time have indicated the possibility of future studies on axonal preparations from whole brain. Correlated biochemical data strongly suggest chemical differences in fractions rich in axonal segments in contrast to those enriched in synaptosomes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00237913
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    Der Einflu\ der Durchblutung auf die Wasser- und Salzresorption im Jejunum der Ratte (1970)
    Springer
    Naunyn-Schmiedeberg's archives of pharmacology 265 (1970), S. 425-441 
    Details
    ISSN: 1432-1912
    Keywords: Intestinal Blood Flow ; Water Absorption ; Solute Absorption ; Jejunum ; Rat ; Darmdurchblutung ; Wasserresorption ; Salzresorption ; Jejunum ; Ratte
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary 1. The lumen of jejunal loops in rats anaesthetised with urethane were perfused with hypo-, iso- and hypertonic ringer, mannitol or glucose ringer solution. The blood flow (venous outflow), water, solute and glucose net flux were measured in the loop. 2. Lowering the blood flow from 1 ml/min/g wet tissue to 0.2–0.3 ml/min/g caused the solute and glucose net flux to decrease; the water net flux (positive or negative) was also decreased if non-isotonie solutions were used, but remained unchanged when isotonic solutions were perfused. 3. When the blood flow was raised from 0.2–0.3 ml/min/g to 1 ml/min/g the net flux of glucose, solute and water usually remained low. Using hypotonic solutions the water net flux changed to negative values, that is, fluid was passing into the lumen. 4. The influenee of intestinal blood flow on water, solute and glueose absorption can be referred to 3 factors: a) drainage by blood, b) inhibition of active transport mechanisms by anoxia during low blood flow, and c) damage to the villi as a result of insufficient blood flow.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00997078
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    A note on the effect of trifluoroacetate on the growth of rat liver (1970)
    Springer
    Naunyn-Schmiedeberg's archives of pharmacology 265 (1970), S. 474-475 
    Details
    ISSN: 1432-1912
    Keywords: Trifluoroacetic Acid ; Trifluoroacetate ; Rat ; Liver Weight ; TrifluoressigsÄure ; Trifluoroacetat ; Ratte ; Lebergewicht
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The influence of trifluoroacetic acid (TFAA) and sodium trifluoroacetate (TFA) on the liver weight to body weight ratio was measured in rats by two methods. When TFA or TFAA were added to the drinking water at a concentrate of 1 Normal, rats became dehydrated and a slight increase in the ratio was noted after ten days. However, when these substances were administered to rats (1 ml per day) by gastric intubation no change in the ratio was noted after eight days.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00997081
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    The effects of noradrenaline on rat's behaviour (1970)
    Springer
    Psychopharmacology 16 (1970), S. 369-374 
    Details
    ISSN: 1432-2072
    Keywords: Noradrenaline ; Intraventricular Injection ; Rat ; Behaviour
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The behavioural effects of NA injected without narcosis into the lateral brain ventricle of the rats were studied with two different techniques. Rats were classified according their normal level of exploratory activity into three groups: high, medium and low. It was shown that NA in a dose of 10 μg increased locomotor activity only in animals of low activity; a dose of 50 μg increased locomotor activity in all the animals; and a dose of 200 μg induced a complete abolition of locomotor activity and a stuporose syndrome lasting 2 hours. The evidence that NA in some experimental conditions increases locomotor activity of rats supports the hypothesis that NA regulates processes in the central nervous system which stimulate behaviour.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00404847
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    Psychopharmacological action of nicotinamide adenine dinucleotide (1970)
    Springer
    Psychopharmacology 17 (1970), S. 198-202 
    Details
    ISSN: 1432-2072
    Keywords: Nicotinamide Adenine Dinucleotide ; Variable Interval Schedule ; Sidman Avoidance ; Sedative Effects ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The effect of nicotinamide adenine dinucleotide (NAD) upon the response rates in variable interval and Sidman Avoidance schedules was studied in rats. Bar pressing on the variable interval reinforcement schedule was significantly reduced during the 15 min following the administration of 10 mg/kg i.p. and during the 60 min following the administration of 30 mg/kg NAD. Response rates on the Sidman Avoidance schedule were significantly reduced during the 60 min following the administration of 100 mg/kg NAD.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00402710
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    Differential effects of nicotine and tobacco smoke alkaloids on swimming endurance in the rat (1970)
    Springer
    Psychopharmacology 18 (1970), S. 300-304 
    Details
    ISSN: 1432-2072
    Keywords: Rat ; Swimming Endurance ; Nicotine ; Tobacco Alkaloids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The swimming endurance of rats in a water tub was measured until the animals submerged for two seconds under the water surface. The total alkaloid fraction extracted from cigarette smoke produced deterioration of performance in doses of 0.05 to 0.2 mg/kg, whereas pure nicotine (0.1 and 0.2 mg/kg), as well as nicotine pretreated analogously to the extraction process of the total alkaloids produced performance improvements.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00412675
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    Light microscopic observations on the possible retinohypothalamic projection in the rat (1970)
    Springer
    Experimental brain research 11 (1970), S. 515-527 
    Details
    ISSN: 1432-1106
    Keywords: Visual pathway ; Hypothalamus ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Following unilateral retinal destruction in rats the existence of a direct retinohypothalamic pathway was investigated using the Nauta and the Fink-Heimer methods. Critical analysis of the Fink-Heimer stained sections of the experimental animals suggests that fibres leaving the optic pathway pass to various hypothalamic nuclei: 1. Fibres from the dorsorostral part of the chiasm pass through the lamina terminalis and appear to end in the preoptic and anterior hypothalamic nuclei. 2. Fibres leaving the ventrocaudal border of the chiasm and optic tract pass to the arcuate and ventromedial hypothalamic nuclei. 3. From the dorsocaudal part of the chiasm fibres pass to the suprachiasmatic and ventromedial hypothalamic nuclei. 4. From the caudal part of the inferior bundle of the accessory optic tract fibres pass to the premamillary ventral nucleus. The amount of apparently terminal degeneration in the preoptic, anterior hypothalamic, suprachiasmatic and premamillary nuclei was small. Heavier terminal degeneration appears to be present in the arcuate and ventromedial hypothalamic nuclei.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00233972
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    Electronic Resource
    Electron microscopic observations on the possible retinohypothalamic projection in the rat (1970)
    Springer
    Experimental brain research 11 (1970), S. 528-538 
    Details
    ISSN: 1432-1106
    Keywords: Visual pathway ; Hypothalamus ; Arcuate nucleus ; Rat ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Degenerating nerve fibres and boutons were searched with the aid of the electron microscope in the arcuate nucleus of rats 2–7 days after bilateral destruction of the retina. In the arcuate nucleus of the control animals as well as in the operated animals, 4 types of boutons were distinguished on the basis of vesicular contents and glial ensheathment. In the operated animals changes interpreted as degenerating were found in small myelinated axons and boutons of type II (boutons containing both synaptic and granular vesicles). The changes were similar to those described in the literature as the “dark” type of degeneration in experimentally interrupted axons and boutons. Similar changes were not found in the unoperated animals. The conclusion is reached, that a small number of fibres of the optic tract reach the arcuate nucleus to terminate here.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00233973
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  • 77
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    Über die enterale Resorption von metallischem Quecksilber (1970)
    Springer
    Archives of toxicology 26 (1970), S. 203-209 
    Details
    ISSN: 1432-0738
    Keywords: Mercury ; Enteral Absorption ; Neutron Activation Analysis ; Rat ; Quecksilber ; enterale Resorption ; Neutronenaktivierungs analyse ; Ratte
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Durch Neutronenaktivierungsanalyse wird nachgewiesen, daß bei der Eatte von symptomlos vertragenem metallischem Quecksilber, oral zugeführt, ein geringer Anteil resorbiert wird, welcher zu einer Erhöhung der Queeksilberwerte in Blut und Niere auf reichlich das 10fache der Norm führt.
    Notes: Summary It was demonstrated by means of neutron activation analysis that a small portion of orally administered metallic mercury is absorbed by rats. The animals tolerated this without visible symptoms, yet it resulted in a 10-fold or greater increase above normal values of mercury in blood and kidneys.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00578041
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  • 78
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    Reninabgabe aus Nierenschnitten in Abhängigkeit von der Elektrolytzusammensetzung des Mediums (1970)
    Springer
    Pflügers Archiv 321 (1970), S. 67-82 
    Details
    ISSN: 1432-2013
    Keywords: Renin ; Angiotensin ; Electrolytes ; Kidney Slices ; Rat ; Renin ; Angiotensin ; Elektrolyte ; Nierenschnitte ; Ratte
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Nierenschnitte von Wistarratten wurden in NaCl-Lösungen oder in Ringer-Bicarbonat(bzw. Phosphat-)-Puffern mit 0,1 g Glucose/l für 2 Std bei 37° C aerob inkubiert. 0,1 ml Medium wurde mit 1,9 ml Rinder-Reninsubstratlösung in Anwesenheit von Diisopropylfluorophosphat und EDTA bei pH 5,7 und 37° C für 2 Std inkubiert. Der Angiotensingehalt des Inkubates wurde im Blutdrucktest der Ratte bestimmt. In verschieden konzentrierten NaCl-Lösungen nimmt die Reninabgabe von 150–67 m Val/l (Na+) kontinuierlich ab. Auch in Ringer-Lösungen mit abnehmender NaCl-Konzentration und konstanter Konzentration der übrigen Elektrolyte findet sich zwischen 120 und 50 m Val/l (Na+) ein steiler Abfall der Reninabgabe. Bei Konstanterhaltung der Osmolarität durch zunehmenden Ersatz des NaCl durch Cholinchlorid oder Mannit vermindert sich die Steilheit der (Na+)-Abhängigkeit der Reninabgabe. Ersatz des NaCl im Bicarbonatpuffer durch verschiedene Konzentrationen von NaBr ergibt die gleichen Befunde wie mit NaCl. Bei vollständigem Ersatz des NaCl im Puffer durch verschiedene Konzentrationen von Chlolinchorid oder Mannit ist die Konzentrationsabhängigkeit der Reninabgabe weniger deutlich, bei Ersatz durch KCl nicht mehr signifikant. Die (Na+)-Abhängigkeit der Reninabgabe aus Nierenschnitten scheint nicht vom aeroben Stoffwechsel abhängig zu sein.
    Notes: Summary Rat kidney slices were incubated in saline solutions or in Ringer-bicarbonate (resp. phosphate-)-buffers containing 0.1 g/l glucose for 2 hrs at 37° C. Subsequently, 0.1 ml of the medium was incubated with 1.9 ml of ox renin-substrate solution, pH 5.7, for 2 hrs at 37° C in the presence of diisopropylfluorophosphate and EDTA. The angiotensin formed was measured by rat blood pressure assay. Renin release from kidney slices decreases markedly in NaCl-solutions between Na+-concentrations of 150 meq/l and 67 meq/l. Renin release decreases likewise in modified Ringer-solutions containing decreasing amounts of NaCl or NaBr (between 120 meq/l and 50 meq/l Na+), when the concentrations of other electrolytes are held constant. The (Na+)-dependence of renin release is less marked when the osmolarity of the medium is held constant by stepwise substitution of NaCl cholinchloride. Complete substitution of NaCl by different concentrations of cholin-chloride or mannitol diminishes the dependence of renin release on the osmolarity of the medium. Variation of KCl-concentration in the absence of NaCl does not influence renin release significantly. The (Na+)-dependence of renin release does not seem to depend on aerobic conditions.
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    Studies on adrenergic mechanisms in the action of desmethyl imipramine (DMI) (1970)
    Springer
    Psychopharmacology 17 (1970), S. 234-241 
    Details
    ISSN: 1432-2072
    Keywords: Specific Inhibition of Amine Synthesis ; Brain ; DMI and RO 4-1284 Antagonism ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The influence of α-methyl tyrosine and diethyldithiocarbamate which specifically inhibit noradrenaline synthesis and the influence of p-chlorophenylalanine which inhibits 5-hydroxytryptamine synthesis in the brain on the antagonism between desmethylimipramine and RO 4-1284 was studied. It was shown that both substances which inhibit noradrenaline synthesis abolish the behavioural antagonism between DMI and RO 4-1284, and p-chlorophenylalanine is without effect on this antagonism. The evidence shows that DMI antagonises the action of a benzoquinolizine derivative by the participation of adrenergic mechanisms.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00402083
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  • 80
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    Effect of hippocampal and neocortical ablation on scopolamine-induced activity in the rat (1970)
    Springer
    Psychopharmacology 17 (1970), S. 289-301 
    Details
    ISSN: 1432-2072
    Keywords: Scopolamine ; Hippocampus ; Activity ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract To test the hypothesis that the hippocampus may be an important site of action for anticholinergic drugs, scopolamine was administered to rats with hippocampal lesions produced by aspiration and to appropriate control groups, and their activity measured. The experimental design was a four-way analysis of variance with three lesion groups, three drug levels, eight measurements in a two-hour session, and four weeks. At the two higher drug doses (0.20 and 1.0 mg/kg), rats with hippocampal or cortical lesions had significantly greater activity than the sham operates (p〈0.01 and p〈0.05, respectively). A group of Ss with electrolytic hippocampal lesions tested at 0.20 mg/kg scopolamine had transitory activity increases. Therefore the hippocampus is not necessary for the motor activating effects of the drug nor is its ablation unique in producing increases in drug-induced activity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00404234
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    The effect of nicotine and total alkaloids extracted from cigarette smoke on avoidance behavior in rats under extinction procedure (1970)
    Springer
    Psychopharmacology 18 (1970), S. 305-313 
    Details
    ISSN: 1432-2072
    Keywords: Rat ; Avoidance ; Extinction ; Nicotine ; Tobacco Alkaloids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The effect of nicotine and total alkaloids extracted from smoke on the avoidance behavior of rats under extinction procedure has been measured in an experiment extended over a period of three months. There was no significant difference between the two substances, with both inhibiting the extinction of avoidance response to approximately the same degree. Significance against the control was achieved with all treatments, the effect being significantly greater with the dose of 0.2 mg/kg than with the two doses of 0.1 or 0.05 mg/kg.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00412676
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  • 82
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    A new method for studying the effect of drugs on the testes of rats (1970)
    Springer
    Naunyn-Schmiedeberg's archives of pharmacology 266 (1970), S. 195-198 
    Details
    ISSN: 1432-1912
    Keywords: Rat ; Testis ; Semen ; Ejaculation ; Pharmacological Tests
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Each of 12 rats was eleetroejaculated 15 times. The semen plug was liquefied in 1% alpha-chymotrypsin, enabling the spermatozoa to be counted. Spermatozoa counts averaged 7,700,000 per ejaculate with a wide range. In 5 of the animals the coagulating glands and the seminal vesicles were surgically removed. After the removal of the glands the rats were electroejaculated 10 times. The still partly coagulated semen was dissolved in 1% alpha-chymotrypsin. The total ejaculate now showed a mean count of 18,600,000 spermatozoa with only a small range. By counting and comparing the number of spermatozoa in the ejaculate in treated and untreated rats with seminal vesicles and coagulating glands removed the effect of drugs on the testes can be examined.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00997652
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  • 83
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    Zwei elektronenmikroskopisch unterscheidbare Formen sekundärer sensorischer Endigungen in einer Muskelspindel der Ratte (1970)
    Springer
    Cell & tissue research 110 (1970), S. 97-107 
    Details
    ISSN: 1432-0878
    Keywords: Muscle Spindles ; Different types of sensory endings ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung In einer Muskelspindel der Ratte, die an einer Serie alternierender Semidünn- und Ultradünnquerschnitte untersucht wurde, wurden zwei benachbart an „nuclear chain“-Fasern gelegene Nervenendformationen unterschiedlicher Bauweise festgestellt. Die beiden Endformationen sind mit ein und derselben Nervenfaser der Gruppe II verbunden und daher als Bestandteile einer sekundären sensorischen Endigung zu betrachten. Die Nervenendformation an einer der beiden „nuclear chain“-Fasern hat anulo-spirale Form. Die Nervenendformation an der anderen „nuclear chain“-Faser weist am Querschnittsbild eine Anzahl von Axonen auf, die zwischen Plasmalemm und Basalmembran der intrafusalen Muskelfaser eng aneinanderliegen. Nicht alle Axonquerschnitte stehen in direktem Kontakt mit der intrafusalen Faser. Das terminale Axon scheint sieh nach Eintritt unter die Basalmembran der intrafusalen Faser mehrfach in relativ dünne Äste unregelmäßigen Verlaufs zu teilen. Diese Form der Endigung könnte ein Korrelat der sog. „flower spray“-Endigung im Sinne Ruffinis (1898) darstellen. Die beiden morphologisch unterschiedlichen Endformationen innerhalb der sekundären Endigung gleichen einander und den Endformationen der primären Endigung bezüglich folgender Ultrastrukturmerkmale: 1. Es besteht synaptischer Kontakt zwischen Axon und intrafusaler Muskelfaser (synaptischer Spalt durchschnittlich 200 Å) ohne Zwischenlagerung von Basalmembranmaterial; 2. die terminalen Axonabschnitte liegen direkt unter der Basalmembran der intrafusalen Muskelfaser und sind nicht von Schwannschen Zellen bedeckt; 3. Mangel an synaptischen Bläschen; 4. desmosomenartige Verhaftungen zwischen Zellmembranen von Axon und intrafusaler Faser; 5. dyadenartige Anlagerungen des sarkoplasmatischen Retikulums an die Zellmembran der intrafusalen Faser im Bereich des synaptischen Spaltes. Nach unseren derzeitigen Vorstellungen sprechen diese Ultrastrukturmerkmale für eine rezeptorische Natur der beschriebenen Nervenendigungen.
    Notes: Summary In a rat muscle spindle transversally cut into a series of alternating semithin and ultrathin sections, two different forms of nerve terminations were found on two neighbouring nuclear chain fibres. The two nerve terminations were connected to the same group II nerve fibre and are consequently constituents of one particular secondary sensory ending. The nerve termination on one of the two nuclear chain fibres is of the anulo-spiral type. The nerve termination on the second nuclear chain fibre shows a number of axons lying closely together between plasma membrane and basement membrane of the intrafusal muscle fibre. Not all of these axons are in direct contact with the intrafusal fibre. The terminating nerve fibre seems to be divided into several branches of rather small diameters and irregular courses. It is suggested that this kind of termination could be a correlate of the so-called “flower spray” type of sensory endings in muscle spindles. The two morphologically different nerve terminations in the secondary ending have the following ultrastructural characteristics in common with those of the primary ending: 1) Synaptic contact between axon and intrafusal muscle fibre (synaptic gap about 200 Å) without interposition of basement membrane material; 2) terminal axons located beneath the basement membrane layer of intrafusal muscle fibres without covering by Schwann cells; 3) lack of synaptic vesicles; 4) desmosome-like structures between plasma membranes of axon and intrafusal muscle fibre, and 5) dyads of the sarcoplasmic reticulum adjacent to the synaptic cleft. According to present knowledge these features indicate that all of these endings are sensory ones.
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    Ultrastructure du lobe postérieur de l'hypophyse du rat et ses modifications au cours de l'excrétion de vasopressine (1970)
    Springer
    Cell & tissue research 108 (1970), S. 357-379 
    Details
    ISSN: 1432-0878
    Keywords: Neurohypophysis ; Rat ; Vasopressin release ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Résumé Le lobe postérieur de l'hypophyse a été examiné sur coupes ultrafines chez 45 rats (24 normaux, 17 déshydratés respectivement 1, 2 et 4 jours, et 4 anesthésiés à l'éther), après fixation au glutaraldéhyde ou selon la méthode de Karnovsky, post-fixation osmiée et double contraste à l'acétate d'uranyle et au citrate de plomb. Des fibres neurosécrétoires sombres et claires sont reconnaissables, mais les données manquent encore pour interpréter cette différence. Des densifications juxta-membranaires sont observées dans les terminaisons neurosécrétoires là où se trouvent des amas de microvésicules. Des fibres neurosécrétoires traversent parfois des pituicytes; il est aussi souvent observé des fibres, chargées de granules, libres dans un espace péricapillaire. Les espaces péricapillaires, ramifiés en tous sens loin des vaisseaux, développent une large surface de contact avec les extrémités nerveuses et les prolongements de pituicytes. Dans des conditions techniques bien contrôlées, la déshydratation n'entraîne pas de modifications appréciables des granules de neurosécrétat après 24 h. Ensuite les granules sont diminués en nombre, de façon très considérable le 4e jour; mais les granules restants ont un contenu dense normal; jamais il n'a été observé d'aspects de ≪granules vides ≫. Après anesthésie prolongée à l'éther, il n'y a aucune modification visible ni du nombre, ni de la densité des granules. Ces observations sont discutées quant au mécanisme de l'excrétion de vasopressine; elles sont en faveur de l'existence de deux pools hormonaux, l'un libre et rapidement disponible, l'autre plus fortement lié et certainement contenu dans les granules jouant le rôle de réserve. Toutefois un mécanisme d'exocytose granulaire ne peut être formellement exclu.
    Notes: Summary Hypophysial neural lobes of 45 rats (24 controls, 17 dehydrated resp. 1, 2 and 4 days, and 4 ether anesthetized) were fixed either with glutaraldehyde or according to Karnovsky and post-fixed in osmium tetroxyde; ultrathin sections were stained by uranyl acetate and lead citrate. Dark and clear neurosecretory fibres were observed, but sufficient data are still lacking for a valuable interpretation of these aspects. Juxta-membraneous densifications are visible in limited areas of neurosecretory terminals where clusters of microvesicles are present. Neurosecretory fibres happen to be completely encircled by pituioyte cytoplasm; fibres loaded with elementary granules are frequently encountered running free in a pericapillary space. Pericapillary spaces stretch out branching far away from vessels, resulting in a widespread contact with nerve terminals and pituicyte processes. In accurately controlled technical conditions, dehydratation does not result in any noticeable change of neurosecretory granules after 24 h. A decrease of the number of granules follows and is extremely conspicuous after 4 days; though, remaining granules keep a normal dense content, and aspects of “empty granules” have never been observed. After prolonged ether anesthesia, no visible change either in number or electron density of granules was observed. These findings are discussed in consideration of the mechanism of vasopressin release; they support the hypothesis of two hormonal pools, one of which would be free and rapidly available for release, the other being more tightly bound and certainly located in granules representing a storage site. Though granular exocytosis cannot be absolutely excluded.
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    URL: http://dx.doi.org/10.1007/BF00336525
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    Quantitativ-morphologische Untersuchungen an Herzmuskelzellen von normalen und hypoxischen Ratten (1970)
    Springer
    Cell & tissue research 109 (1970), S. 384-397 
    Details
    ISSN: 1432-0878
    Keywords: Heart muscle cells ; Electron microscopy ; Quantitative ; Asphyxia ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Normale und hypoxische Herzmuskelzellen aus der Wand des linken Ventrikels der Ratte wurden quantitativ-morphologisch anhand von elektronenmikroskopischen Längsschnitten nach Perfusionsfixierung untersucht. In normalen Zellen waren alle Myofibrillen relaxiert, die mittlere Sarcomerlänge betrug 2,2 μm. Die Schnittfläche wurde zu 55% von Myofibrillen, zu 27% von Mitochondrien und zu 18% von Grundplasma und Reticulum eingenommen. Die zwischen den Myofibrillen liegenden Mitochondrien waren längsoval und im Mittel 2,3mal so lang wie breit. Es bestand kein Unterschied zwischen subendokardial und subepikardial gelegenen Zellen. 10 min nach Erstickung der Tiere waren in den sonst unauffälligen Muskelzellen die Glycogengranula vermindert. Nach 20 min führte die Hypoxie zu einer Zunahme der relativen Schnittfläche der Mitochondrien um etwa 16% und zu einer beginnenden Kontraktur der Myofibrillen (Sarcomerlänge 2,0 μm). 20 min Hypoxie in Hypothermie (25–30°C intrathorakal) veränderte die normale Zellstruktur dagegen kaum. Wenn die Herzen während der 20 min dauernden Hypoxie in Normothermie mit einer procainhaltigen sauerstoff- und glucosefreien Blutersatzlösung durchspült wurden, waren die Myofibrillen relaxiert, die Schwellung der Mitochondrien dagegen wurde nicht reduziert. 30 min nach Erstickung wurde die Kontraktur stärker (Sarcomerlänge 1,7 μm). Nach 60 min bildeten sich Superkontraktionsknoten, einzelne Myofibrillen waren in Höhe der I-Bänder unterbrochen. Die Cristae der Mitochondrien wichen auseinander, die Schnittfläche der Mitochondrien hatte um 27% zugenommen. Während in Normotherapie eine Asphyxie des Tieres bereits nach 10 min die Herzmuskelzellen funktionell schwer schädigt, ist die Schädigung morphologisch erst nach 20 min eindeutig. Das bedeutet, daß für die elektronenmikroskopische Präparation eine Hypoxie von unter 10 min bedeutungslos ist. Hinsichtlich der morphologischen Manifestationszeit für die Unterbrechung der Sauerstoffversorgung stimmen unsere Befunde an Herzmuskelzellen gut mit vergleichbaren Angaben an Leberzellen überein.
    Notes: Summary In heart muscle cells of the left ventricle of rats the distribution of cell organelles and their reaction to hypoxia were investigated by electron microscopy. In normal hearts fixed by perfusion with aldehydes, the mean sarcomere length was 2.2 μm. 27% of the longitudinal sectional area was occupied by mitochondria, 55% by myofibrils and 18% by sarcoplasmic reticulum and ground plasm. The mitochondria situated in rows between the fibrils were oval and measured 2.3 times more in length than in width. There was no difference between cells from subendocardial and subepicardial regions. 10 min hypoxia (complete occlusion of the trachea) did not affect the appearance of muscle cells but diminished the number of glycogen granules. After 20 minutes the area occupied by mitochondria was increased by 16%, the mitochondria between the myofibrils were more spherical and only 1.5 times longer than wide. The sarcomeres shortened to 2.0 μm. With hypothermia (25–30°C) hypoxia of 20 minutes duration did not affect the cell structure. Perfusion of the heart by a saline solution, which contained procaine but neither oxygen nor glucose, for 20 minutes prevented shortening of the sarcomeres but not swelling of the mitochondria. 30 minutes after occlusion of the trachea the myofibrils shortened to a sarcomere length of 1.7 μm. After 60 minutes irregularly and excessively contracted myofibrils appeared and some sarcomeres were interrupted at the level of the I-bands. In some of the swollen mitochondria the cristae were widely separated. The increase of the area occupied by mitochondria was 27%. Asphyxia affects heart muscle cells severely with respect to function within 10 min, but morphologically it takes 20 min before a definite effect can be noticed. As to the time after which lack of oxygen is manifested morphologically, our results are consistent with findings in liver cells.
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    Morphology of superficial postnatal cerebral cortex with special reference to synapses (1970)
    Springer
    Cell & tissue research 110 (1970), S. 540-558 
    Details
    ISSN: 1432-0878
    Keywords: Cerebral cortex ; Synapses ; Rat ; Postnatal differentiation ; Morphology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The ultrastructure of layers I and II of the motor cerebral cortex of rat brain has been studied at birth, 4, 7 and 14 days postnatal and in the adult. Compared with the adult, neonatal rat motor cortex exhibited a large extracellular space which decreases with increasing age. At all stages studied the neurons were seen to contain the organelles usually found in adult neurons. Growth cones were present in decreasing numbers up to 14 days old. Synapses were detectable at birth and there was an obvious increase in their number throughout the postnatal development. At the earliest stages studied there was a lack of specialization characteristic of the adult. Many synapses were either avesicular or relatively so and lacked the high degree of modification of adult pre- and postsynaptic membranes. By 7 days after birth many synapses existed which in all morphological respects resembled those of the adult, and by 14 days, the majority were of the adult type. These findings, particularly with reference to the postnatal development of synapses, have been discussed in relation to the known electrophysiological findings.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00330103
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    Origine embryonnaire et différenciation sécrétoire des cellules à calcitonine (cellules C) dans la thyroïde foetale du rat (1970)
    Springer
    Cell & tissue research 106 (1970), S. 251-268 
    Details
    ISSN: 1432-0878
    Keywords: C cells ; Thyroid ; Rat ; Ultimobranchial origin ; Differentiation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Résumé Nos observations confirment l'origine ultimobranchiale des cellules C de la thyroïde du rat. L'évolution des corps ultimobranchiaux (C.U.B.) a été étudiée à partir du 16ème jour de gestation, stade où ils fusionnent avec l'ébauche thyroïdienne. Dès le 17ème jour, les premières cellules C se différencient dans le C.U.B. inclus dans la thyroïde. Au 18ème jour, elles sont plus nombreuses et commencent à se disperser dans les cordons thyroïdiens. La migration des cellules C est particulièrement importante au 19ème jour. A partir du 20ème jour, les signes d'activité sécrétoire s'intensifient très nettement. A la fin de la vie foetale, les cellules C sont comparables aux cellules C adultes. La differenciation des cellules parathyroïdiennes précède nettement celle des cellules C.
    Notes: Summary Our observations confirm the ultimobranchial origin of the C cells of the rat thyroid. We studied the development of the ultimobranchial body from the 16th day of pregnancy, when there is fusion with the thyroidian anlage, onwards. As early as the 17th day, the first C cells differentiate in the ultimobranchial body which is included into the thyroid. On the 18th day, they are more numerous and start to scatter throughout the thyroidian cords. The migration of the C cells is especially obvious on the 19th day. From the 20th day onwards, there is marked increase in secretory activity. At the end of the foetal life, the C cells resemble those of the adult. The differentiation of the parathyroid cells significantly precedes that of the C cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00335742
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  • 88
    Electronic Resource
    Electronic Resource
    Fine structure of trypsin-dissociated rat adrenal cells (1970)
    Springer
    Cell & tissue research 107 (1970), S. 447-453 
    Details
    ISSN: 1432-0878
    Keywords: Adrenal cortex ; Rat ; Trypsin-dissociation ; ACTH stimulation ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Electron micrographs of trypsin-dissociated rat adrenal showed predominantly intact rounded cells without internal damage. The population contained cells from the glomerular, intermediary and fascicular zones with cells from the zona fasciculata predominant. The presence or absence of cells from the reticular zone could not be determined. Cells from the medullary zone were absent. The addition of adrenocorticotropic hormone (ACTH) to the cellular suspension for 2 hours produced corticosterone. However, these stimulated cells did not display any significant ultrastructural change.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00335433
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  • 89
    Electronic Resource
    Electronic Resource
    On the epithelium of the rat pituitary residual lumen (1970)
    Springer
    Cell & tissue research 108 (1970), S. 487-500 
    Details
    ISSN: 1432-0878
    Keywords: Pituitary gland ; Residual lumen ; Epithelial cells ; Fine structure ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Histology, enzyme histochemistry, and electron microscopy of the epithelium covering the rat pituitary residual lumen was studied. The anterior and posterior epithelium have similar histological and histochemical appearance, although the posterior epithelium shows stronger enzyme reactions for an esterase and many dehydrogenases. Electron microscopic studies reveal that both epithelia form a continuous lining. Anterior epithelium is in immediate contact with the interstitial spaces of the anterior lobe, while the posterior epithelium is separated from the intermediate lobe by a continuous basement lamina. The cytological features of both epithelia are also remarkably similar with scanty rough-surfaced endoplasmic reticulum, inconspicuous Golgi apparatus, round or oval mitochondria, and moderate number of lysosomal bodies. The apical surface of these cells is covered by microvilli and in some, especially posterior epithelial cells, by numerous cilia. Anterior epithelial cells and the apical portions of the posterior epithelial cells contain a number of large vacuoles with material possibly related to the colloid within the residual lumen. Electron microscopic findings suggest that both epithelia are possibly active in transfer and/or disposal of the colloid material rather than being secretory themselves. Enzyme histochemical findings support the hypothesis of an active role of these cells in metabolic processes related to phagocytosis. Based on these observations the colloid seems to be the product of the anterior lobe function.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00339655
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  • 90
    Electronic Resource
    Electronic Resource
    Quantitative studies of postnatal changes in synapses in rat superficial motor cerebral cortex (1970)
    Springer
    Cell & tissue research 110 (1970), S. 559-568 
    Details
    ISSN: 1432-0878
    Keywords: Cerebral cortex ; Rat ; Synapses ; Postnatal ; Quantitation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Quantitation of synapses at different postnatal ages has been undertaken in the cerebral cortex of the rat. In this study axial ratios of presynaptic bags, proportion of cortex occupied by presynaptic bags and numbers of synapses per unit volume of cortex have been estimated. Observations on synaptic vesicle packing densities have also been made. Synaptic bags become increasingly spherical up to 7 days of age and become more elongated thereafter. The proportion of cortex occupied by presynaptic bags increases rapidly up to 7 days of age and then at a decelerated rate up to maturity. The number of synapses per unit volume increases slowly over the first four days after which there is a rapid increase to 14 days, followed by a decelerated rate. The average presynaptic bag shows marked changes in volume with increasing age which indicate the probability of two stages of synaptic development. This two stage development is further reflected in the estimates on vesicle packing densities. The implications of the results are discussed in relationship to changes in functional activity of the cortex during postnatal development.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00330104
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  • 91
    Electronic Resource
    Electronic Resource
    Elektronenmikroskopische Untersuchungen zur Inselregeneration im Rattenpankreas nach subtotaler Pankreatektomie (1970)
    Springer
    Cell & tissue research 110 (1970), S. 569-587 
    Details
    ISSN: 1432-0878
    Keywords: Subtotal Pancreatectomy ; Rat ; Islet Regeneration ; Excretory Duct System
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Männliche Ratten wurden bei einem Gewicht von 80–100 g subtotal pankreatektomiert (5% Rest) und zu verschiedenen Zeitpunkten nach der Operation abgetötet. Bei diesen wie bei unbehandelten Kontrolltieren wurde die Neubildung von Inselgewebe aus dem exkretorischen Gangsystem elektronenmikroskopisch untersucht. — Es konnte gezeigt werden, daß die Inselneubildung von Gangzellen ausgeht, welche ein auffallend helles Zytoplasma besitzen und samt ihren Kernen größer und abgerundeter sind als ihre Nachbarzellen. Diese Zellen wandern vom Lumen ab, wobei die entstehende Lücke zum Lumen zu jeweils durch eine seitliche Ausdehnung des Zytoplasmas benachbarter Gangzellen geschlossen wird. Die abgewanderten hellen Zellen, die mit den lichtmikroskopisch beschriebenen „trüben Zellen“ identisch sein dürften, haben die Fähigkeit, sich zu Alpha-oder Beta-Zellen zu differenzieren. Dabei werden sie größer und das Zytoplasma dunkler. Sie behalten aber gleichzeitig einige Besonderheiten, die auf ihre Herkunft hinweisen. Die Regeneration des Inselgewebes, die vorwiegend von den zentroacinären Zellen ausgeht, setzt bald nach der Operation stark ein, schwächt sich dann aber kontinuierlich ab. In den immer ineffektiver werdenden Regenerationsvorgang werden zunehmend größere Gangabschnitte einbezogen. Die weitgehende Erschöpfung der Regenerationsfähigkeit fällt mit dem Auftreten eines manifesten Diabetes zwischen dem 40. und 60. Tag p.op. zusammen.
    Notes: Summary Male rats were subtotally pancreatectomized at a weight of 80–100 g according to the method of Foglia (5% of the total pancreas remaining). The animals were killed at different intervals after. In these rats, as well as in healthy controls, the new formation of islets out of the excretory duct system was studied by electron microscopy. Obviously the new formation of islets takes origin from certain cells of the ductular system which show a light cytoplasm and are larger and rounder than their neighbouring cells. The latter goes for their nuclei, too, even to a larger degree. These light cells migrate away from the ductular lumen and the gaps resulting from this are closed by long and thin processes of the cytoplasm of their neighbouring duct cells coming in contact with each other. The cells having migrated away seem to have an ability to differentiate into alphaor beta-cells. Most probably they are identical with the “trübe Zellen” described by light-microscopists. The higher the degree of differentiation in these cells is, the darker becomes their cytoplasm. But they still keep for some time morphological aspects indicating their origin from ductular cells. An intense regeneration of islet tissue starts soon after the operation from the centroacinar cells but it decreases continuously after. As the process of islet regeneration becomes more and more ineffective, an increasing number of larger excretory ducts are involved in it. The exhaustion of the ability for regeneration is correlated exactly in time with the onset of a manifest diabetes between the 40 th and 60 th day after operation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00330105
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  • 92
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    Electronic Resource
    Electron microscopic studies on the oocyte maturation in cultures of juvenile rat ovaries (1970)
    Springer
    Cell & tissue research 111 (1970), S. 364-378 
    Details
    ISSN: 1432-0878
    Keywords: Rat ; Oocyte maturation ; in vitro studies
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary After cultivation in a modified Trowell-system (organ ciilture) oocytes of 12- to 40-day-old rats were studied under the light and electron microscope. The cultivation period was 72 hrs. Egg cells from primary and early secondary follicles which are characterized by the lack of a zona pellucida and by one or at most two very large Golgi's complexes located near the nucleus do not show any alterations. The other egg cells are characterized by membrane packages which can otherwise only be observed in mature egg cells right before or after the ovulation. Meiotic processes or the formation of polar bodies are only seldom to be seen. These egg cells are surrounded by a thick zona pellucida. From these findings the following conclusions can be drawn: 1. In organ cultures meiotic processes are prevented by the inhibitory influence of follicle cells (Foote and Thibault, 1969). 2. A cytoplasmic maturation proceeds independently. 3. In-vivo an inhibitory factor influences the cytoplasmic maturation. This inhibition is not observed in the organ culture. Thus we can be sure that it develops outside the ovary. 4. The synthesis of the zona pellucida continues in-vitro.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00342488
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  • 93
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    Electronic Resource
    Cellular localization of 3H-oestradiol in the hypothalamus (1970)
    Springer
    Cell & tissue research 104 (1970), S. 572-581 
    Details
    ISSN: 1432-0878
    Keywords: Hypothalamus ; Rat ; Cellular Localization of 3H-Oestradiol ; Autoradiography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The hypothalamus of male and female rats, given 0.3 μg/100 g body weight of 6.7-3H-oestradiol-17β and killed 1 hour after the injection, was examined by autoradiography in order to 1) localize the areas and the cells involved in the uptake of the hormone, and 2) study the intracellular localization of the labelled material. Only nerve cells contained radioactive material while glial and ependymal cells were not significantly labelled. In the anterior hypothalamus, labelled nerve cells were concentrated in areas corresponding to nucleus preopticus medialis and nucleus preopticus, pars suprachiasmatica. The nucleus supraopticus was unlabelled. In the medial basal hypothalamus, neurons corresponding to the nucleus arcuatus and the lateral part of the nucleus ventromedialis showed marked labelling. No significant labelling was observed in the nucleus paraventricularis, pars magnocellularis. Although the individual nerve cells varied in their extent of labelling, the major proportion of the silver grains were consistently concentrated over the nuclei. Castration was not found to influence the results. The findings were essentially the same in male and female rats and appear to suggest that oestradiol exerts a direct effect on nerve cells in certain hypothalamic areas.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00335379
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  • 94
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    Electronic Resource
    The uptake of 3H-oestradiol by the anterior hypophysis and hypothalamus of male and female rats (1970)
    Springer
    Cell & tissue research 104 (1970), S. 582-596 
    Details
    ISSN: 1432-0878
    Keywords: Anterior hypophysis ; Hypothalamus ; Rat ; Uptake of 3H-Oestradiol
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The anterior hypophysis and hypothalamus of male and female rats given 3H-oestradiol were examined with regard to 1) the kinetics of the uptake of the radioactive material, 2) the chemical nature of the labelled material, and 3) the influence of non-labelled oestradiol-17β, oestradiol-17α and testosterone on the uptake of 3H-oestradiol. The anterior hypophysis was found to concentrate and retain oestradiol in basically the same manner in male and female rats. The pattern of the uptake was similar to that of the uterus and vagina, with a concentration peak 2 hours after the injection. Non-target tissues such as cerebral cortex, liver and blood attained their maximum uptake already 15 minutes after the injection. Thereafter the concentration gradually decreased. The ratio between the concentration of labelled material in the anterior hypophysis and brain cortex gradually increased until a peak was reached at 8 hours in both sexes. In the female, the concentration of labelled material in the anterior hypophysis was then 106.3 times greater than in the brain cortex, while in the male the ratio was 63.2. In the hypothalamus the uptake followed a pattern similar to that of the brain cortex. However, in the former the concentration of labelled material was consistently greater than in the latter. At maximum uptake, registered 4 hours after the injection, the concentration was about two times greater in the hypothalamus than in the cerebral cortex. The neurohypophysis contained, on an average, 1/6 of the amount of radioactive material registered in the anterior lobe one hour after the injection, but it was about two times greater than in the brain cortex. Isolation and identification of the radioactive material in the anterior hypophysis and hypothalamus showed that in both sexes nearly all of it was chemically unchanged oestradiol. Graded doses of non-labelled oestradiol-17β were found to decrease the uptake of 3H-oestradiol in the anterior hypophysis and hypothalamus almost linearly, while the concentration of labelled material in the brain was unaltered. Oestradiol-17α and testosterone were without significant effect on both the pituitary and hypothalamic accumulation of 3H-oestradiol. Therefore, a limited number of binding sites, with a high degree of specificity for oestradiol, appear to exist in both tissues. The results were essentially the same in male and female rats.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00335380
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  • 95
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    Electronic Resource
    Ultrastructural changes in the median eminence of the rat following deafferentation of the basal hypothalamus (1970)
    Springer
    Cell & tissue research 105 (1970), S. 1-32 
    Details
    ISSN: 1432-0878
    Keywords: Median eminence ; Rat ; Deafferentation ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary This report concerns a light and electron microscopic investigation of the median eminence and dorsal infundibular stem of the rat following surgical isolation (deafferentation). Using a modification of the Halász technique, the basal hypothalamus, including the arcuate nucleus and median eminence were surgically isolated from surrounding structures. Special attention was directed to the contact (external) zone of the median eminence and rostral infundibulum where tuberohypophyseal axons as well as ependymal cell processes abut upon the abluminal basement membrane of the portal perivascular space. The results of this study to date suggest that 9, 20, and 40 days following surgical isolation, there is a distinct increase in the population of tuberohypophyseal dense core vesicles. It is suggested that deafferentation abolishes inhibitory and excitatory input that serves to modify the cellular dynamics of tuberohypophyseal neurosecretory elements. Comments are also made on the presence of cistern-like structures in the lateral median eminence; the presence of vesicle-like inclusions in terminal ependymal processes is discussed in relationship to the role that ependyma may play in linking the third ventricle with the adenohypophysis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00340562
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  • 96
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    Electronic Resource
    Cellular localization of 3H-oestradiol in the hypophysis (1970)
    Springer
    Cell & tissue research 104 (1970), S. 597-614 
    Details
    ISSN: 1432-0878
    Keywords: Hypophysis ; Rat ; Localization of 3H-Oestradiol ; Autoradiography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The pituitaries of male and female rats given 0.3 μg of 6.7-3H-oestradiol-17β per 100 g body weight were examined by autoradiography in order to 1) identify the cells responsible for the uptake of the hormone, 2) determine the intracellular distribution of the hormone and quantify the proportions localized within the cytoplasm and nucleus by silver grain counting, and 3) see if sex differences existed in the cellular and intracellular distribution of the hormone. The animals were killed at intervals varying from 1 minute to 8 hours following intravenous or intramuscular injection. A large proportion of pituitary cells having the morphologic characteristics of acidophils, basophils and chromophobes contained radioactive material. Castration cells and acidophils of gonadectomized and lactating rats showed marked labelling. In male and female rats killed 10 minutes after intravenous injection, 84.4 and 83.6 per cent of the cells were labelled. One hour after intramuscular injection, 86.6 and 76.1 per cent of the cells were labelled in males and females, respectively. Thus, a small proportion of the cells remained unlabelled. Labelled cells showed silver grains both in the cytoplasm and over the cell nuclei, but the major proportion of the radioactive material was invariably associated with the cell nuclei in all cell types and at all time intervals. About 65 per cent of the radioactive material was associated with the cell nuclei in animals killed five minutes or one hour after intravenous or intramuscular injection of the hormone. The silver grains appeared to be randomly distributed in both the cytoplasm and over the cell nuclei. In the intermediate lobe and the neurohypophysis, only sparse labelling with random distribution was observed. At the border between the intermediate lobe and the neurohypophysis, labelling of single cells or clusters of cells similar to those in the adenohypophysis was found. The results, which were essentially the same in male and female rats, appear to indicate a direct effect of oestradiol at the pituitary level.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00335381
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  • 97
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    Electronic Resource
    Monoamine fluorescence in the median eminence of foetal, neonatal and adult rats (1970)
    Springer
    Cell & tissue research 104 (1970), S. 541-556 
    Details
    ISSN: 1432-0878
    Keywords: Median eminence ; Rat ; Monoamine fluorescence ; Output of pars distalis hormones
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The distribution of monoamines in the median eminence of foetal, neonatal and adult rats has been studied with the Falck-Hillarp fluorescence histochemical technique in preparations in which the hypothalamus and pituitary gland were in continuity. In adult rats monoamine fluorescence is present throughout the median eminence, including the anatomical stem. In foetal rats a few sagittally-directed varicose fluorescent fibres are seen in the median eminence. In neonatal rats palisade layer fluorescence in appreciable amounts is first seen on the fourth day in the region of the anatomical stem. By the eighth day it has spread throughout the palisade layer of the entire median eminence, and by the end of the third week it has attained adult intensity. The appearance of monoamines in the median eminence, the development of a functional hypophysial portal system, and a changing pattern of development of function in both the hypothalamo-pituitary-adrenal and hypothalamo-pituitary-thyroid axes thus all appear to be contemporaneous events. It is suggested that the monoamines of the median eminence represent part of a neural system influencing the output of pars distalis hormones, in particular of ACTH and TSH secretion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00335377
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  • 98
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    Electronic Resource
    Altersabhängige Veränderungen im histochemischen Reaktionsmuster des Thymus bei Wistar-Ratten (1970)
    Springer
    Cell & tissue research 104 (1970), S. 138-148 
    Details
    ISSN: 1432-0878
    Keywords: Thymus ; Rat ; Involution ; Histochemical pattern
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Makrophagen und entodermale Retikulumzellen samt Ausläufern lassen sich im Thymus der Wistar-Ratte durch den histochemischen Nachweis der sauren Phosphatase und der unspezifischen Esterase selektiv darstellen, während die Lymphozyten nicht reagieren. Häufigkeit und Verteilung der Retikulumzellen und Makrophagen lassen altersabhängige Unterschiede erkennen: Auf Kosten der Retikulumzellen nimmt die Zahl der Makrophagen mit fortschreitendem Lebensalter zu. Bei geschlechtsreifen Tieren bilden sie einen Wall an der Mark-Rinden-Grenze. Die Bedeutung der sauren Phosphatase in entodermalen Retikulumzellen wird unter der Annahme diskutiert, daß es sich um lysosomengebundenes Ferment handelt. Lysosomen sind im involvierenden Thymus wahrscheinlich an autolytischen oder phagozytotischen Prozessen beteiligt. Es stellt sich die Frage, ob Lysosomen in entodermalen Retikulumzellen des Thymus neugeborener Tiere in die Abgabe einer humoral wirksamen Substanz eingreifen können.
    Notes: Summary Macrophages and entodermal reticulum cells in the thymus of Wistar rats contrast by their rich contents of acid phosphatase and unspecific esterase activity with the negativ reacting lymphocytes. Frequency and distribution of the cells vary according to different stages of age: at cost of the entodermal reticular cells the number of macrophages increases with progressive age. In sexually mature animals they form a typical layer at the corticomedullary junction. The functional meaning of acid phosphatase in the entodermal reticulum cells is discussed under the assumption that this enzyme is lysosomebound. In the involuting thymus lysosomes are involved in autolytic or phagocytic processes. The question arises wether lysosomes in entodermal reticulum cells in the thymus of newborn animals can take part in the control of an humoral function of the thymus.
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    URL: http://dx.doi.org/10.1007/BF00340055
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  • 99
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    Electronic Resource
    Über Zusammenhänge zwischen der Senkung des arteriellen und des portalen Blutdrucks und dem Strömungsstillstand in den Lebersinusoiden (1970)
    Springer
    Clinical and experimental medicine 152 (1970), S. 244-250 
    Details
    ISSN: 1591-9528
    Keywords: Hepatic microcirculation ; Intravital microscopy ; Haemorrhagic hypotension ; Rat ; Mikrozirkulation der Leber ; Intravitalmikroskopie ; hämorrhagische Hypotension ; Ratte
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Die Auswirkungen eines massiven Blutverlustes auf die Blutströmung in den Sinusoiden der Leber bis zum Zusammenbruch der Mikrozirkulation sind fÜr das Ausmaß und die Reversibilität der akuten Blutungsfolgen wesentlich. Während kontrollierten Entblutens wurde bei der Ratte an einem vorgelagerten Leberlappen die Mikrozirkulation intravitalmikroskopisch im Durchlicht beobachtet. Durch die Anwendung einer Modifikation vorliegender Methoden waren gleichzeitige Druckmessungen in der Vena portae und in einer Arteria carotis möglich. Die gewonnenen Ergebnisse bestätigen die Brauchbarkeit der Versuchsanordnung. Zu einem vollkommenen Strömungsstillstand in allen sichtbaren Sinusoiden kommt es nach langsamem Entbluten von durchschnittlich 30% des Blutvolumens. Der Druck in der Arteria carotis ist dabei von 89,25 auf 21,4 mm Hg, der portale Druck von 92,3 auf 31,4 mm H2O abgefallen. Die Korrelation zwischen dem arteriellen und dem portalen Druck bleibt während dieses Abfallens erhalten.
    Notes: Summary The sequels of a massive haemorrhage on the blood flow in the sinusoids of the liver until the microcirculation breaks finally down, are essential for the degree and the reversibility of acute complications of the haemorrhage. The microcirculation of the exposed rat liver has been observed by intravital microscopy by means of transillumination during controlled desanguination. A modification of methods previously used made it possible to measure simultaneously systemic and portal blood pressure by portal and carotic arterial catheters. The results obtained proved the reliability of the method. A complete flowstop in all visible sinusoids has been observed after slow drainage of 30% of the blood volume. At this stage the blood pressure in the carotic artery has decreased from 89.25 to 21.4 mm Hg and the portal pressure from 92.3 to 31.4 mm water. The correlation between the arterial and the portal blood pressure remained unchanged during this decrease.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02046406
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  • 100
    Electronic Resource
    Electronic Resource
    Zur Frage der Gold-Dekorporierung durch Penicillamin (1970)
    Springer
    Clinical and experimental medicine 152 (1970), S. 352-355 
    Details
    ISSN: 1591-9528
    Keywords: Aurothiomalate ; Penicillamine ; Diethylenetriaminepentaacetate ; Rat ; Aurothiomalat ; Penicillamin ; Diäthylentriaminpentaacetat ; Ratte
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Es wurde der Einfluß der D- und L-Isomeren von Penicillamin sowie von Diäthylentriaminpentaacetat auf die Organverteilung von Gold bei der Ratte untersucht. Während Diäthylentriaminpentaacetat sich als unwirksam erwies, wird Gold durch Penicillamin in nur geringem Maße mobilisiert. Der klinische Wert von Penicillamin als mögliches Antidot bei der Chrysotherapie erscheint somit fraglich.
    Notes: Summary The influence of the D- and L-isomeres of penicillamine as well as of diethylenetriaminepentaacetate on the distribution of gold in the organs of the rat was studied. Whereas diethylenetriaminepentaacetate proved to be ineffective, penicillamine brings about a marginal mobilization of gold only. The clinical value of penicillamine as possible antidote in chrysotherapy, therefore, is doubtful.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02044918
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