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  • Articles: DFG German National Licenses  (137)
  • 1985-1989
  • 1970-1974  (137)
  • 1974  (64)
  • 1972  (73)
  • Rat  (137)
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  • Articles: DFG German National Licenses  (137)
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Years
  • 1985-1989
  • 1970-1974  (137)
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Keywords
  • 1
    Electronic Resource
    Electronic Resource
    The contribution of solvent drag to the intestinal absorption of the acidic drugs benzoic acid and salicylic acid from the jejunum of the rat (1974)
    Springer
    Naunyn-Schmiedeberg's archives of pharmacology 281 (1974), S. 197-217 
    Details
    ISSN: 1432-1912
    Keywords: Intestinal Absorption ; Acidic Drugs ; Benzoic Acid ; Salicylic Acid ; Solvent Drag ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary 1. Jejunal loops of anaesthetized rats were perfused with hypotonic, isotonic and hypertonic buffered solutions containing 14C-labelled benzoic acid and salicylic acid at pH 6.2 and 2.2. The blood flow of the loop was maintained at an intermediate rate (0.78–0.97 ml min−1 g−1). The water net flux was determined by polyethylene glycol as non-absorbable marker and amounted up to +31 or-27 μl min−1 g−1. 2. A positive water net flux (leaving the gut lumen) increased the appearance rate of benzoic acid and salicylic acid in the intestinal venous blood by maximally 47 and 41%, a negative water net flux (entering the gut lumen) diminished the appearance rate by 28 and 37%. 3. The experimental data were analysed by means of a kinetic model. The parameters of the model were the epithelial permeability kF D , the serosal permeability k S F S and the sieving coefficient Φ=1−σ. The epithelial permeability was 0.115 and 0.107 for benzoic acid and salicylic acid at pH 6.2 and increased to 0.163 and 0.185 ml min−1 g−1 at pH 2.2. The serosal permeability was assumed to be identical for neutral and acidic pH and amounted to 0.046 and 0.112 ml min−1 g−1 indicating that a certain proportion of the absorbed drugs was transferred to the serosal side. The sieving coefficient was 2.92 and 2.31 at neutral pH and 3.79 and 3.56 at acidic pH. 4. The main resistance to the absorption of the two drugs is the epithelial membrane, since the effective blood flow and the unstirred layer are not ratelimiting. The high sieving coefficient for the two drugs is interpreted as indicating an interaction of drug and water molecules inside the lipid part of the cell membrane (which contains at least 30% water). The electric potential across the gut wall may contribute to the size of the sieving coefficient, if the drug molecules permeate also in the ionized form. 5. Using acidic perfusion solutions the epithelial permeability and the sieving coefficient were increased by the factor 1.3–1.5. This increase can be explained sufficiently by a facilitated entrance of the unionized drug molecules into the epithelial membrane.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00503498
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  • 2
    Electronic Resource
    Electronic Resource
    Dekorporierung von Thallium durch rhyolithischen Bims bei der Ratte (1974)
    Springer
    Research in experimental medicine 162 (1974), S. 63-66 
    Details
    ISSN: 1433-8580
    Keywords: Rhyolithic pumice ; Ferrihexacyanoferrate (II) ; Thallium ; Rat ; Rhyolithischer Bims ; Ferrihexacyanoferrat (II) ; Thallium ; Ratte
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Orale Verabfolgung von rhyolithischem Bims bewirkt eine herabgesetzte Retention von Thallium in den Geweben. Die Wirksamkeit von Rhyolith zeigt eine ausgeprägte Dosisabhängigkeit, ist aber geringer als die von Ferrihexacyanoferrat (II).
    Notes: Summary Oral administration of rhyolith leads to a diminished retention of thallium by the tissues. The effectiveness of rhyolith markedly depends on its dosage, but is surpassed by that of ferrihexacyanoferrate (II).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01851884
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  • 3
    Electronic Resource
    Electronic Resource
    Untersuchungen zur Beeinflussung der renalen Exkretion von D-Penicillamin bei der Ratte (1974)
    Springer
    Research in experimental medicine 163 (1974), S. 39-45 
    Details
    ISSN: 1433-8580
    Keywords: D-penicillamine ; Acetazolamide ; Renal excretion ; Rat ; D-Penicillamin ; Acetazolamid ; Renale Exkretion ; Ratte
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Die renale Ausscheidung von D-Penicillamin läßt sich weder durch Probenecid noch durch N-Methylnicotinamid oder NH4Cl beeinflussen. Acetazolamid bewirkt eine erhöhte Retention von D-Penicillamin in Haut und Muskulatur und hemmt dessen renale Ausscheidung, hat jedoch keinen Effekt auf die Dekorporationswirksamkeit von D-Penicillamin bei60Co. Die möglichen Ursachen des Einflusses von Acetazolamid auf die Verteilung und Exkretion von D-Penicillamin werden diskutiert.
    Notes: Summary The renal excretion of D-penicillamine is not influenced by probenecid, N-methylnicotinamide, and NH4Cl. Acetazolamide brings about an increased retention of D-penicillamine in skin and muscles and lowers its urinary excretion, but does not exhibit any influence on the efficacy of D-penicillamine in removing internally deposited60Co. The possible causes for the action of acetazolamide on the distribution and excretion of D-penicillamine are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01851444
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  • 4
    Electronic Resource
    Electronic Resource
    Experimental uremic syndrome in the rat (1974)
    Springer
    Research in experimental medicine 163 (1974), S. 341-349 
    Details
    ISSN: 1433-8580
    Keywords: Experimental uremic syndrome ; Rat ; 5/6 reduction of renal parenchyma ; Serum- and urine analysis ; Secondary hyperparathyroidism ; Experimentelles urämisches Syndrom ; Ratte ; 5/6-Reduktion des Nierenparenchyms ; Serum- und Urinanalyse ; Sekundärer Hyperparathyreoidismus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Durch eine 5/6-Nierenparenchymreduktion wurde an Ratten ein chronisches, durch bilaterale Nephrektomie ein akutes urämisches Syndrom erzeugt. Beim chronischen urämischen Syndrom erwiesen sich in der Serumanalyse die Konzentrationen des Harnstoffstickstoffs und des Kreatinins als wesentlich erhöht. Darüber hinaus bestanden alle chemischen Zeichen eines sekundären Hyperparathyreoidismus. Die Tiere litten außerdem an einer schweren Poly-, Protein- und Albuminurie. Überraschenderweise war aber der Spiegel des totalen Serumproteins gegenüber den Kontrolltieren erhöht. Das experimentelle chronische urämische Syndrom stellt eine extreme Adaptation der regulatorischen Aktivität des Nierenrestparenchyms und des ganzen Organismus dar. Beim akuten, durch bilaterale Nephrektomie erzeugten urämischen Syndrom besteht dagegen ein schweres Versagen der Regulation des Wasser- und Elektrolytstoffwechsels, welches innert 50–60 Std zum Tode der Versuchstiere führt.
    Notes: Summary A chronic uremic syndrome was induced by 5/6 nephrectomy and an acute uremic syndrome by bilateral nephrectomy in rats. In the chronic uremic syndrome serum and urine analysis revealed a highly increased serum level of BUN and creatinine. Furthermore, all chemical signs typical of secondary hyperparathyroidism were present. The animals suffered from severe polyuria, protein- and albuminuria but, surprisingly, the total serum protein was increased. The experimental chronic uremic syndrome is, in essence, a syndrome of “extreme adaptation” of the regulatory activity of remnant renal parenchyma and of the whole body. In contrast, the acute uremic syndrome induced by bilateral nephrectomy is a severe disorder of water and electrolyte metabolism resulting in death of the experimental animals after 50–60 hrs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01851521
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  • 5
    Electronic Resource
    Electronic Resource
    Ultrastruktur des proximalen Nierentubulus nach Behandlung mit einem Pentosanschwefelsäureester (1974)
    Springer
    Research in experimental medicine 164 (1974), S. 19-33 
    Details
    ISSN: 1433-8580
    Keywords: Kidney ; Rat ; Ultrastructure ; Proximal tubule degeneration ; Heparinoid ; Niere ; Ratte ; Ultrastruktur ; Proximale Tubulusdegeneration ; Heparinoid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung 24 Std nach i.v. Injektion von 100 mg/kg eines Pentosanschwefelsäureesters (SP 54) sind in der Nierenrinde von Ratten schwere degenerative Veränderungen der proximalen Tubuluszellen zu beobachten. Das Tubulusepithel ist flachkubisch umgewandelt, das Tubuluslumen ist angefüllt mit ausgestoßenen Mitochondrien und anderen Zelltrümmern. Am stärksten betroffen ist die Pars contorta des proximalen Tubulus. Weniger stark veränderte Tubuluszellen sind angefüllt mit Cytosomen, die eine spezifische Feinstruktur haben oder gehäuft gegenüber Kontrollen Cytoplasmaprotrusionen in das Tubuluslumen aufweisen. Die akute Tubulusdegeneration ist ein spezifischer Effekt von SP 54. Heparin bewirkt nur eine leichte Schwellung des Tubulusepithels, die häufiger auftritt als bei Kontrolltieren.
    Notes: Summary 24 hrs after the intravenous injection of 100 mg/kg of a Pentosansulfuricacidester (SP 54) severe degenerative alterations are observed in the proximal tubular cells in rat kidney cortex. The tubular epithelium has changed to a single layer of elongated cubic cells. The tubular lumen is filled with cellular debris and extruded mitochondria. The greatest change is seen in the pars contorta of the proximal tubule. Less degenerated tubular cells have a great number of cytosomes with a specific substructure or have cytoplasmatic protrusions into the tubular lumen. This acute tubular degeneration is a specific event after the administration of SP 54 and is not seen after an equimolar dosis of Heparin. Heparin induces only a slight swelling of the tubular epithelium, more frequent seen as in the control animals.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01851961
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  • 6
    Electronic Resource
    Electronic Resource
    Influence of coprophagy prevented by partial immobilization on calcium metabolism and bone morphology in the rat (1974)
    Springer
    Research in experimental medicine 162 (1974), S. 313-321 
    Details
    ISSN: 1433-8580
    Keywords: Coprophagy ; Rat ; Calcium metabolism ; Bone morphology ; Immobilization ; Koprophagie ; Ratte ; Calciumstoffwechsel ; Knochenmorphologie ; Immobilisierung
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Wir untersuchten den Einfluß der Koprophagie auf den Calciumstoffwechsel der Ratte. 20 Versuchstiere wurden durch Einschnüren in einen Lederpanzer an der Koprophagie gehindert und mit 21 Kontrolltieren gleicher Futteraufnahme verglichen. Die Kinetik des Calciumstoffwechsels wurde unter Verwendung von45Ca als Tracer analysiert. Die radiochemischen Resultate wurden mit den histologischen Beobachtungen an den Epi-Metaphysen der Kniegelenke verglichen. Bei den Versuchstieren mit verhinderter Koprophagie fanden wir: 1. ein hochsignifikant niedrigeres Körpergewicht; 2. eine hochsignifikante Verkleinerung folgender Parameter des Calciumstoffwechsels: Compartmentgröße und Austauschrate zwischen ihnen, Calciumausscheidungsraten, Knochenan- und -abbau; 3. eine Verschmälerung der Wachstumsfuge, verkürzte und verschmälerte primäre Knochenbälkchen, verminderte sekundäre metaphysäre Spongiosa. Bei 4 Versuchstieren, die in Lederpanzern gehalten, denen jedoch die Koprophagie ermöglicht war, wurden gleichartige histologische Veränderungen wie bei verhinderter Koprophagie festgestellt. Es wird gefolgert, daß die Verhinderung der Koprophagie keinen meßbaren Einfluß auf die Parameter des Calciumstoffwechsels hat.
    Notes: Summary We investigated the influence of coprophagy on the calcium metabolism of the rat. 20 experimental animals were prevented from coprophagy by wrapping them in leather harnesses and compared with 21 controls on the same food intake. Kinetics of calcium metabolism were analysed using45Ca as tracer. The radiochemical results were compared with the histological observations in the epi-metaphyses of the knee joints. In the experimental animals prevented from coprophagy we found: 1. a highly significant lower body weight; 2. a highly significant diminution of the following parameters of calcium metabolism: compartment sizes and exchange rate between them, calcium excretion rates, bone accretion and resorption; 3. a narrower epiphyseal growth cartilage, clearly shorter and narrower primary trabeculae, rarefied secondary metaphyseal trabeculae. Histological alterations identical to those observed with coprophagy prevented, were found in 4 animals kept in harnesses but with coprophagy allowed. It is concluded that prevention of coprophagy has no measurable influence on the parameters of calcium metabolism.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01851702
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  • 7
    Electronic Resource
    Electronic Resource
    Wirkung steigender Pentagastrindosen auf die Säuresekretion des Rattenmagens nach Vagotomie im Perfusionsversuch (1974)
    Springer
    Research in experimental medicine 163 (1974), S. 289-298 
    Details
    ISSN: 1433-8580
    Keywords: Gastrinpentapeptide ; Vagotomy ; Acid secretion ; Perfusion test ; Rat ; Gastrinpentapeptid ; Vagotomie ; Säuresekretion ; Perfusionsversuch ; Ratte
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Bei männlichen Wistarratten wurde eine trunkuläre beiderseitige Vagotomie — ergänzt durch eine Pyloroplastik — angelegt. 4 Wochen später erfolgte in Urethannarkose eine Perfusion des Magens mit 0,9%iger NaCl-Lösung in Anlehnung an die Versuchsanordnung von Ghosh. Die Vollständigkeit der Vagotomie bestätigte ein Hypoglykämietest. Im Abstand von 150 min erhielten die Tiere — in 6 Gruppen zu je 10 Ratten — 2 × 50, 100, 150, 200, 250 oder 300 µg/kg KG Pentagastrin subcutan. Die statistisch ausgewerteten Ergebnisse lassen sich wie folgt zusammenfassen: 1. Die nach dem Abklingen der Wirkung der ersten Pentagastrininjektion wiederholte Pentagastringabe in derselben Dosis ergab bei allen Dosierungen eine höhere Säuresekretion als nach der ersten Injektion. 2. Ein Maximum der sezernierten Säuremenge wurde bei einer Dosis von 100 µg/kg KG erreicht. Bei höherer Dosierung des Pentagastrins nahm die Menge der sezernierten Säure signifikant ab.
    Notes: Summary Male Wistar rats were subjected to a complete bilateral vagotomy and a pyloroplasty. 4 weeks later followed in urethane anesthesia a continuous perfusion of the stomach with 0.9% saline according to the rat preparation of Ghosh. The completeness of the gastric vagotomy was confirmed by a postoperative insulin test. In an interval of 150 min the rats — in 6 groups of 10 rats each — were two times injected pentagastrin subcutaneously in dose rates of 50, 100, 150, 250 or 300 µg/kg body weight. The statistical confirmed results can be summarized as followed: 1. The second injection of pentagastrin — 150 min after the first — in the same dose produced a significantly higher gastric acid secretion in the rat all dose rates in comparison to the first injection. 2. The maximal gastric acid secretion was produced by a dose rate of 100 µg/kg body weight of pentagastrin. At higher dose rates of pentagastrin there was a significant reduction of gastric acid secretion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01851516
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  • 8
    Electronic Resource
    Electronic Resource
    Functional changes of the neuronal and glial elements at the surface of the external layer of the median eminence (1974)
    Springer
    Anatomy and embryology 143 (1974), S. 255-262 
    Details
    ISSN: 1432-0568
    Keywords: Median eminence ; Neuro-vascular contacts ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The vascular surface of the external layer of the median eminence is composed of neuronal and glial processes. Neuro-vascular contacts are supposed to be necessary for the release of hypophysiotropic neurohormones. The present study confirms the hypothesis that the relative extent of neuro-vascular and glio-vascular contacts varies under different functional conditions of the tubero-hypophysial system. Morphometric analysis shows that in normal rats about 20% of the surface is lined by neuro-vascular contacts. After bilateral adrenalectomy the neuro-vascular contacts are enlarged to 40% whereas application of an extract of stalk-median eminence tissue is followed by a decrease of the neuro-vascular contacts to 15%. The implications of these results are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00519869
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  • 9
    Electronic Resource
    Electronic Resource
    Über die Entstehung der Basalmembranlabyrinthe (1974)
    Springer
    Anatomy and embryology 143 (1974), S. 185-203 
    Details
    ISSN: 1432-0568
    Keywords: Basement membrane labyrinths ; Cerebral ventricles ; Development ; Rat ; Light and Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Gehirne embryonaler und neugeborener Ratten wurden im Hinblick auf die frühe Entstehung der Basalmembranlabyrinthe licht- und elektronenmikroskopisch untersucht. 1. An den Hirngefäßen embryonaler Ratten der 2. und 3. Fetalwoche kommen zwar bereits Basalmembranen vor, sub- oder interependymale Basalmembranlabyrinthe fehlen aber noch. 2. Basalmembranlabyrinthe werden mit der Perjodsäure-Bisulfit-Aldehydthionin-Methode lichtmikroskopisch ab dem 20. Tag an umschriebenen Stellen des Ventrikelsystems gefunden; elektronenmikroskopisch treten die ersten Basalmembranlabyrinthe am 12. postnatalen Tag auf. 3. Die ersten interependymalen Labyrinthe erscheinen in Erweiterung der interependymalen Spalten in der Nähe von ausgedehnten Golgifeldern. Der Labyrinthinhalt besteht aus einer feinflockigen Substanz. Diese liegt wolkenartig angehäuft gegenüber bogenförmigen Ausbuchtungen lokal verbreiterter Zellmembranen. In Ausbildung begriffene Labyrinthe zeigen eine lamelläre Gliederung. 4. Ab dem 12. postnatalen Tag treten auch an den Basalmembranen der pericapillären Zellen, in denen lichtmikroskopisch Glykoproteide nachweisbar sind, zapfenartige Duplikaturen auf. Die Zapfen sind überwiegend ependymwärts gerichtet. Verbindungen zwischen den Basalmembranzapfen und den interependymalen Basalmembranlabyrinthen werden in den ersten 30 Lebenstagen nicht gefunden. 5. Sowohl an den die interependymalen Labyrinthe begrenzenden Plasmalemmata als auch am Plasmalemm der pericapillären Zellen fallen Stachelsaumbläschen auf, deren Membran in die Zellwand eingebaut wird. Ihr Inhalt scheint in die entstehenden Labyrinthe entleert zu werden. 6. Da sich an den tubulären Enden der Doppellamellen der Dictyosomen Strukturen befinden, die den Stachelsaumbläschen morphologisch gleichen, und da im Bereich des Golgifeldes und im Cytoplasma zwischen diesem und der Zellwand zahlreiche Stachelsaumbläschen liegen, wird angenommen, daß das Material der Basalmembranlabyrinthe dem Golgiapparat entstammt und vermittels der Stachelsaumbläschen zur Zellwand transportiert wird. Bogenförmige Ausbuchtungen lokal verbreiterter Zellmembranen im Bereich entstehender Labyrinthe werden als Reste von Stachelsaumbläschen aufgefaßt, deren Membran in die Zellwand eingebaut wurde. 7. Da die phylogenetische Zunahme des Hirnmantels mit einem Verlust der langen in das Gehirn reichenden basalen Ependymfortsätze an den größten Teilen der Ventrikelwand einhergeht und niedere Tiere noch nicht über Basalmenbranlabyrinthe verfügen, wird angenommen, daß diese die Transportfunktion für Stoffe aus dem Liquor übernommen haben, die vordem nach allgemeiner Auffassung über die langen Fortsätze in das Gehirn gelangten.
    Notes: Summary The brains of embryonic and new-born rats were investigated by means of light- and electron microscopy with regard to the early formation of basement membrane labyrinths. 1. Though basement membranes are already found around the brain capillaries of embryonic rats from the 2nd week of pregnancy, sub- and interependymal basement membrane labyrinths are still absent. 2. Basement membrane labyrinths, being demonstrable for light microscopy by a periodicacid-bisulfite-aldehydethionin-method, appear around the 20th day after birth at certain places of the ventricular system. By means of electron microscopy, basement membrane labyrinths have first been detected at the 12th postnatal day. 3. The earliest interependymal basement membrane labyrinths are found in enlargements of the interependymal spaces near a distended Golgi apparatus. The contents of the labyrinths, being composed of a loose flocculent material, are of a lamellar structure. In the intercellular space the material is situated opposite bow-shaped excavations of local broadenings of cell membranes. 4. From the 12th postnatal day, plug-like duplications of basement membranes occur at the ependymal side of the pericapillar cells, which contain glycoproteids. The plugs of basement membranes are directed towards the ependymal layer. No connections between the interependymal basement membrane labyrinths and the plugs of pericapillary basement membranes exist within the first 30 days of life. 5. At the plasmalemma of ependymal cells bordering the interependymal labyrinths, and at the cell membrane of pericapillary cells, coated vesicles are to be found, which are fused with the cell membrane. The contents of these vesicles seem to be released into the developing labyrinths. 6. At the tubular ends of dictyosomes, coated vesicle-like structures can be demonstrated. In the environment of the Golgi apparatus many coated vesicles are situated; they can even be found between the Golgi apparatus and the walls of labyrinthś. Therefore the coated vesicles are considered to be transport vesicles, transporting the material which is formed in the Golgi apparatus towards the cell membrane. 7. Since the phylogenetic increase of the brain mantle is accompanied by loss of the long processes of ependymal cells that reach far into the brain, and since lower animals have no basement membrane labyrinths, it is suggested that the basement membrane labyrinths have a transport functions for material from the cerebrospinal fluid which in lower animals is assumed to be transported by the long processes of ependymal cells.
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    URL: http://dx.doi.org/10.1007/BF00525769
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  • 10
    Electronic Resource
    Electronic Resource
    Feinbau und Passageverhalten der Capillaren im Subcommissuralorgan der Ratte (1974)
    Springer
    Anatomy and embryology 144 (1974), S. 101-122 
    Details
    ISSN: 1432-0568
    Keywords: Subcommissural organ ; Periodically structured bodies ; Vascular permeability ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Die Capillaren des Subcommissuralorgans (SCO) und deren Umgebung wurden an 38 adulten Sprague-Dawley- und an 6 adulten Wistar-Ratten nach Perfusionsfixierung im Elektronenmikroskop untersucht. Saure Mucopolysaccharide wurden mit Alcianblau und Rutheniumrot, Glykoproteide mittels der Perjodsäure-Silbermethenamin-Reaktion nachgewiesen. Der Stofftransport in den Capillaren wurde mit Meerrettichperoxidase (MRP) als Tracer untersucht. Die subcommissuralen Capillaren besitzen ein ungefenstertes, vesikelarmes Endothel, das einer einfachen, durchgehenden Basallamina aufsitzt. In der Umgebung der Capillaren finden sich neben periodisch strukturierten Körpern (PSK) vereinzelt Basallamina-Labyrinthe und regellos angeordnete kollagene Mikrofibrillen. Mit der Perjodsäure-Silbermethenamin-Reaktion lassen sich Glykoproteide im Bereich der Basallamina und der nach außen anschließenden Intercellularspalten darstellen, vereinzelt auch — teils im Bereich der Basallamina, teils ihr eng angeschlossen — Gruppen parallel angeordneter, linearer Strukturen mit einem periodischen Streifenmuster. Saure Mucopolysaccharide sind nur in der lumenseitigen Glykokalyx des Capillarendothels nachweisbar. Der Aufbau der PSK aus Kollagenfilamenten erscheint sehr wahrscheinlich. Vermutungen zur Entstehung dieser hochgeordneten Strukturen werden vorgebracht. Die Traceruntersuchungen ergeben, daß unter normalen Bedingungen in den subcommissuralen Capillaren eine Blut-Hirn-Schranke (BHS) für MRP besteht, die im Endothel lokalisiert ist. Bei einem Teil der Sprague-Dawley-Ratten ist die Barriere geschädigt und der Tracer gelangt über cytopemptische Vesikel in Basallamina und Gefäßumgebung. Der Basallamina und den PSK kommt in solchen Fällen keine besondere Schranken- oder Verteilerfunktion zu. Offenbar bewirkt bei manchen Sprague-Dawley-Ratten die MRP-Injektion über die Freisetzung von endogenen biogenen Aminen aus Mastzellen eine Schädigung der BHS, was bei Wistar-Ratten nicht eintritt.
    Notes: Summary The capillaries of the subcommissural organ (SCO) and their adjacent structures of 38 adult Sprague-Dawley rats and 6 adult Wistar rats were examined in the electron microscope after perfusion fixation. Acid mucopolysaccharides were shown by Alcian Blue and Ruthenium Red, glycoproteins were identified by the periodic acid-silver methenamine technique. Horseradish peroxidase (MRP) was used as a tracer for vascular permeability. The subcommissural organ's capillaries are characterized by an unfenestrated, continuous endothelium, showing only few vesicles; the endothelium is surrounded by a single, continuous basal lamina. Adjacent to the capillaries wall there are “periodically structured bodies” (PSK), labyrinths of the basal lamina and irregularly arranged collagen fibrils. Glycoproteins are found in the basal lamina and within the intercellular clefts; in a few cases groups of parallel arranged, periodically banded linear structures are seen within or adjacent to the basal lamina. Acid mucopolysaccharides are found within the endocapillary layer of capillaries. It seems very likely that PSK represent an atypical arrangement of collagen fibrils. The formation of these structures is discussed. Under normal conditions, MRP, after intravenous injection, does not enter the perivascular space surrounding the capillaries of the SCO. A blood-brain barrier exists at the level of the capillary endothelium. In a few Sprague-Dawley rats MRP seems to alter the permeability of the capillaries. Reaction product of MRP is found within endothelial vesicles and the adjacent structures. It appears that the basal lamina and the PSK are not involved in the barrier mechanism or regulation of transport. In Sprague-Dawley rats intravenous injection of MRP seems to alter the blood-brain barrier by release of endogenous biogenic amines associated with mast cell degranulation. This effect is not observed in Wistar rats.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00518636
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  • 11
    Electronic Resource
    Electronic Resource
    Changes of extent of Neuro-vascular contacts and number of neuro-glial synaptoid contacts in the pituitary posterior lobe of dehydrated rats (1974)
    Springer
    Anatomy and embryology 146 (1974), S. 157-165 
    Details
    ISSN: 1432-0568
    Keywords: Neurohypophysis ; Neuro-vascular contacts ; Neuro-glial synaptoid contacts ; Dehydration ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The posterior lobe of the hypophysis consists of neurosecretory nerve fibres and glial cells, both abutting on the perivascular space of sinusoidal capillaries. Nerve fiberes and glial cells are connected with each other by a high number of synaptoid contacts. Several ultrastructural changes following dehydration have been described by other authors. This study presents the data of some morphometric investigations on normal and dehydrated rats. The relative extent of neuro-vascular contacts is not contstant; it increases significantly from 52 (±2.8)% in normal rats to 67(±1.2)% in rats with a dehydration time of 3 days. Correspondingly the extent of glio-vascular contacts is diminished. In addition, water-deprived animals show a clear increase in the number of neuro-glial synaptoid contacts with 3.1 (±0.4) per square unit nervous tissue compared to 2.1 (±0.3) per Square unit in untreated controls. These results indicate a dynamic motility of neuronal and glial elements depending on functional conditions, as was found for the external layer of the median eminence. Furthermore there seems to be a relationship between secretory activity and the number of neuroglial synaptoid contacts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00315592
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  • 12
    Electronic Resource
    Electronic Resource
    Electron microscopic studies of the innervation of the rat kidney (1974)
    Springer
    Anatomy and embryology 145 (1974), S. 169-186 
    Details
    ISSN: 1432-0568
    Keywords: Renal innervation ; Rat ; Peripheral nerves ; Neuroeffector zones ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung In der Rattenniere werden die muskelzellhaltigen arteriellen Gefäße und der juxtaglomeruläre Apparat innerviert. Blutgefäße mit Pericyten, porenhaltige Capillaren sowie die Tubuli der Rinde und des Markes werden nicht von Nervenfasern begleitet. Ganglienzellen wurden in der Rattenniere nicht beobachtet. Periphere Nerven mit einem ein-bis zweischichtigen Perineurium kommen im paravasalen Gewebe der Interlobar- und Arcuata-Arterien vor; sie enthalten neben zahlreichen marklosen Nervenfasern gewöhnlich auch 2–4 markhaltige. Nervenfaser-Bündel ohne perineurale Scheide finden sich im paravasalen Gewebe der Arcuata- und Interlobular-Arterien. Darüber hinaus sind in unmittelbarer Nachbarschaft der großen Arterien (Interlobar-, Arcuata- und Inter-lobular-arterien) und der Vasa afferentia marklose Nervenfasern und freie Axone vorhanden, die auch die proximalen Abschnitte der Vasa efferentia der subcapsulären und intermediären Rindenschicht begleiten. Im Nierenmark werden die juxtamedullären Vasa efferentia und die Arteriolae rectae innerviert; marklose Nervenfasern und freie Axone sind nur bis zur Außen-Innenstreifen-Grenze nachweisbar. Die Innervation der muskelzellhaltigen arteriellen Gefäße erfolgt durch aufgetriebene Axonabschnitte (Neuroeffektor-Zonen), die vorwiegend agranuläre Vesikel enthalten. Diese Strukturen liegen stets an der Grenze von Adventitia und Media bzw. Elastica externa; zwischen den glatten Muskelzellender Media wurden keine vesikelhaltigen Axonabschnitte gefunden. Als minimaler Abstand zwischen den vesikelhaltigen Axonabschnitten und den von einer Basalmembran umschlossenen glatten Muskelzellen (neuromuskuläre Distanz) wurden 600 Å gemessen. Die vorliegenden Ergebnisse werden mit den fluoreszenzmikroskopischen und histochemischen Untersuchungen über die adrenerge und cholinerge Innervation der Niere verglichen. Die sich aus diesem Vergleich ergebenden Probleme und funktionellen Konsequenzen für die Innervation der Niere sowie die Natur der cholinergen Fasern (afferente oder postganglionäre parasympathische Fasern) werden diskutiert.
    Notes: Summary The innervation of the rat kidney is defined by a system which supplies those arterial blood vessels whose walls contain smooth muscle cells and the juxtaglomerular apparatus. Vessels containing pericytes, or those vessels composed of an endothelium only, as well as the tubules of both the cortex and medulla, are not innervated. Furthermore, ganglion cells do not occur in the rat kidney. The nervous apparatus of the rat kidney consists of peripheral vegetative nerves, ensheathed by a perineurium, with 2–4 myelinated fibers running in the paravasal tissue of the interlobar and arcuate arteries, and of nerve bundles without a perineurial sheath in the paravasal tissue of the arcuate and interlobular arteries. Non-myelinated fibers and free axons occur in the immediate vicinity of the great arteries (interlobar, arcuate, and interlobular) and the vasa afferentia. Nerve fibers and free axons are also seen in the vicinity of only the proximal parts of those vasa efferentia which supply the cortical capillary plexus. The arteriolae rectae of the medulla, and their vasa efferentia, from which they arise, are innervated by non-myelinated fibers and free axons which accompany these arterial vessels only to the boundary of the outer and inner stripe of the outer zone of the medulla. The functional innervation of those vessels with smooth muscle cells results from neuro-effector zones which predominantly show agranular vesicles. These structures were never seen between the smooth muscle cells within the media; the minimum neuromuscular distance was 600 Å. The present findings are correlated with the lightmicroscopically demonstrated adrenergic and cholinergic innervation. The resultant problems and functional consequences of the innervation of the kidney, especially the nature of the cholinergic fibers (afferent or post-ganglionic parasympathetic fibers) are briefly discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00519727
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  • 13
    Electronic Resource
    Electronic Resource
    Neuroembryogenesis in the hippocampal formation of the rat (1974)
    Springer
    Anatomy and embryology 144 (1974), S. 173-186 
    Details
    ISSN: 1432-0568
    Keywords: Hippocampus ; Neurogenesis ; Autoradiography ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summay The prenatal ontogeny of the hippocampus, including the anterior continuation and induseum griseum, was examined in the rat by means of tritiated thymidine autoradiography. The neurons of the anterior continuation formed between the 16th and 18th days of embryonic development. The neurons of the induseum griseum were formed on the 16th and 17th days of gestation. The pyramidal cells of ammon's horn were formed during the 16th, 17th, 18th, and 19th days of embryogenesis but were preceded by the large interneurons of the stratum lacunosum-moleculare which formed on days 15 and 16 of embryonic life. The granule cells of the superficial layers of the stratum granulosum formed during the 20th, 21st, and 22nd days of gestation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00519773
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  • 14
    Electronic Resource
    Electronic Resource
    Morphological types of motor nerve terminals in rat hindlimb muscles, possibly innervating different muscle fiber types (1974)
    Springer
    Anatomy and embryology 144 (1974), S. 237-247 
    Details
    ISSN: 1432-0568
    Keywords: Motor nerve terminals ; Methylene blue stain ; Hindlimb muscles ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary With methylene blue stain, three morphological types of motor nerve terminals were distinguished in hindlimb muscles (extensor digitorum longus, semitendinosus, soleus, and tibialis anterior muscles) of rat. Type A terminals possess many long thin branches with numerous minute swellings, type C ones possess few and thick terminal branches with few large swellings, and type B ones possess intermediate characteristics. The frequencies of occurrence of these types are characteristic for each muscle and differ between different hindlimb muscles. Specifically, soleus muscle contains a much higher proportion of type B terminals than other hindlimb muscles, and the superficial part of tibialis anterior muscle contains a higher proportion of type A terminals and a lower proportion of type C ones than the deep part. The frequencies of occurrence of types A, B, and C terminals closely parallel the frequencies of “white”, “intermediate”, and “red” muscle fibers (as determined by a fat stain), respectively, in the different muscles. As also suggested previously for the diaphragm, it is concluded that three morphological types of motor nerve terminals each may innervate one of three types of muscle fibers.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00522809
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  • 15
    Electronic Resource
    Electronic Resource
    Influence of pantothenic acid deficiency on the amount of CRF-granules in the rat median eminence (1974)
    Springer
    Anatomy and embryology 146 (1974), S. 43-55 
    Details
    ISSN: 1432-0568
    Keywords: Pantothenic acid deficiency ; Corticotropin-releasing factor ; Corticoids ; Histologie ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In the outer layer of the rat median eminence “Gomori-positive” granules occur which are assumed to be the morphological correlate of the corticotropin-releasing factor (CRF). The present study was carried out in order to establish whether the amount of these CRF-granules is influenced by a pantothenic acid (PA)-deficiency, which is known to result in an inhibition of corticosteroid production. Female SPF Wistar rats were used. PA-deficiency was induced by PA-free nutrition and/or administration of the PA-antagonist Ω-methyl-PA. Corresponding experimental groups were given either tap water or a solution of 1% NaCl and 5% glucose in tap water to drink. In rats fed with a diet free of PA and in addition treated with Ω-methyl-PA a severe disturbance of the corticoid balance developed within 6 weeks. The corticosterone production was depressed to about 11–16% of the control values. The adrenal cortex showed necrotic changes which either involved the whole parenchyma or were confined to the inner zones. In those animals in which a subcapsular region, in its extent corresponding to the zona glomerulosa, remained intact, an augmentation of the CRF-granules was observed. In those animals, however, in which the whole adrenal cortex was damaged, the amount of CRF-granules did not increase. Administration of the 1% NaCl containing drinking fluid did not influence the augmentation of the CRF granules, but had a protective effect on the development of necrotic changes in the zona glomerulosa of the adrenal cortex. The findings show that a functional relationship exists between the inner zones of the adrenal cortex and the CRF-granules in the median eminence.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00341382
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  • 16
    Electronic Resource
    Electronic Resource
    Scanning electron microscopy demonstration of cilia in rat intrahepatic bile ducts (1974)
    Springer
    Anatomy and embryology 145 (1974), S. 223-226 
    Details
    ISSN: 1432-0568
    Keywords: Rat ; Liver ; Bile ducts ; Cilia ; Scanning Electron Microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Numerous cilia have been demonstrated by SEM in cells of rat perilobular and portal bile ducts and ductules. Generally these cilia appear as long, cylindrical evaginations of the plasma membrane. Some of them are so long that, curving and twisting at many points, they cross the ductal lumen. It has been suggested that they may be related to a continual mixing up and propulsion of the bile product down the biliary tree.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00519634
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  • 17
    Electronic Resource
    Electronic Resource
    The fine structure of the pulmonary stretch receptor in the rat (1974)
    Springer
    Anatomy and embryology 143 (1974), S. 215-222 
    Details
    ISSN: 1432-0568
    Keywords: Lung ; Stretch receptors ; Light microscopy ; Electron microscopy ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Lappenbronchien (3./6.) (Durchmesser bei mittlerer Inspirationslage 500–700 μ) von männlichen und weiblichen Wistarratten wurden licht- und elektronenmikroskopisch untersucht. Diese Bronchien entsprechen im Wandbau den mittleren Bronchioli größerer Säugetiere. Freie, verzweigte lanzettförmige Terminalfasern werden im Bindegewebe der lamina propria beschrieben. Sie sind in das elastisch muskuläre System der Bronchuswand eingebaut. Die zugehörige markhaltige afferente Nervenfaser ist verzweigt und hat einen Durchmesser von 4–6 μ. Außerdem werden efferente motorische Endigungen an der glatten Bronchialmuskulatur beschrieben. Die freien lanzettförmigen und verzweigten Terminalfasern sind möglicherweise Dehnungsrezeptoren für den Hering Breuer Reflex.
    Notes: Summary In rats the 3rd to 6th bronchi, measuring 500–700 μ in diameter during inspiration, were investigated by light and electron microscopy. The histological appearance of these bronchi is comparable to that of medium sized bronchioles of larger animals. The branched and lanceolate terminals are associated with the connective tissue of the lamina propria and the smooth muscle cell layer. In this way the terminals are bound to the myoelastic system of the bronchial wall. The myelinated afferent fiber is branched and the diameter measures about 4–6 microns. Besides afferent nerve terminals these are numerous efferent endings on the smooth muscle basement laminae. It is supposed that the described receptor represents the pulmonary stretch receptor of the Hering Breuer reflex.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00525771
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  • 18
    Electronic Resource
    Electronic Resource
    Ganglienzellen und paraganglionäre Zellen des Ganglion cervicale superius der Ratte nach Parachlorophenylalanin (pCPA) (1974)
    Springer
    Anatomy and embryology 143 (1974), S. 223-237 
    Details
    ISSN: 1432-0568
    Keywords: Superior cervical ganglion ; Rat ; p-Chlorophenylalanine ; 5-Hydroxytryptamine ; Catecholamine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Die Wirkung von Parachlorophenylalanin (pCPA) auf die paraganglionären Zellen und sympathischen Ganglienzellen des Ganglion cervicale superius der Ratte wurde untersucht. pCPA beeinflußt die sympathischen Nervenzellen im Ganglion cervicale superius geringgradig. Es finden sich unspezifische Veränderungen des Golgi-Apparates und der Nissl-Schollen sowie eine Zunahme von Lysosomen. Bei langdauernder Behandlung degenerieren einzelne Ganglienzellen und Nervenfasern. Die paraganglionären Zellen sind bei Kontrolltieren durch “dense core vesicles” eines einheitlichen Typs charakterisiert. Im Laufe der pCPA-Behandlung finden sich degranulierte paraganglionäre Zellen. Die Degranulation der einzelnen Zellelemente ist jedoch variabel. Bei fluorescenzmikroskopischer Untersuchung zeigt sich nach pCPA-Applikation eine Verschiebung des Fluorescenzspektrums zum grünen Bereich hin. Die bei Kontrollen deutliche Gelbkomponente nimmt nach 6–12 Tagen ab und verschwindet schließlich nach 18–20 Tagen. Es wird diskutiert, ob die fluorescenzmikroskopischen Veränderungen aufgrund einer Hemmung der Serotoninsynthese oder aufgrund einer abgeschwächten Katecholaminsynthese entstehen.
    Notes: Summary The effect of parachlorophenylalanine (pCPA) on the paraganglionic and sympathetic nerve cells of the rat superior cervical ganglion was studied. pCPA influences the sympathetic nerve cells of the cervical ganglion to minimal extent. Unspecific changes of the Golgi apparatus and of the Nissl bodies as well as an increase in the number of lysosomes occur. An extended period of treatment causes degeneration of individual ganglionic cells and nerve fibers. The paraganglionic cells in control animals are characterized by dense core vesicles of an unitary type. During pCPA treatment degranulated paraganglionic cells are to be found. The degranulation of the individual cell elements varies. Fluorescence microscopic study after pCPA application shows a shift of the fluorescence spectrum toward green. The yellow component of the control group decreases after 6 to 12 days and disappears after 18 or 20 days. It is discussed whether the fluorescence microscopic changes are due to the inhibition of serotonine synthesis or to a decrease of the catecholamine synthesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00519867
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  • 19
    Electronic Resource
    Electronic Resource
    Effects of triparanol and 20,25-diazacholesterol in CNS of rat: Morphological and biochemical studies (1974)
    Springer
    Acta neuropathologica 29 (1974), S. 141-156 
    Details
    ISSN: 1432-0533
    Keywords: Cholesterol Inhibitors ; Cerebrum ; Neuronal Inclusions ; Oligodendroglial Degeneration ; Rat ; Abnormal Sterols
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Wistar strain of rats received daily intraperitoneal or oral administration of Triparanol, 250 mg per kg of body weight or 20,25-diazacholesterol either 30 mg or 60 mg per kg of body weight starting at the age of 5 days old. Prominent intracytoplasmic osmiophilic inclusions identical to those found in the rats treated with AY9944 (Suzukiet al., 1973) and degeneration of myelin sheaths and oligodendroglia were seen in the myelinating white matter of rats sacrificed after 3, 6, and 9 injections of Triparanol. In diazacholesterol-treated animals, a few identical inclusions were seen in the cortical neurons but only slight changes were seen in oligodendroglia. Biochemical analysis of the brains of the rats receiving Triparanol revealed a large accumulation ofΔ 7,24-cholestadiene-3β-ol and lesser amounts of desmosterol. The brains of the animals treated with diazacholesterol revealed massive accumulation of desmosterol and a minor sterol component, probablyΔ 5,7,24-cholestatriene-3β-ol. Since AY9944, which results in a massive accumulation of 7 dehydrocholesterol (Δ 5,7-cholestadiene-3β-ol) and two other sterols,Δ 7,24-cholestadiene-3β-ol andΔ 5,7,24-cholestatriene-3β-ol, will produce identical neuropathological lesions to those seen in Triparanol treated animals, and since the lesion produced in diazacholesterol treated animals was very slight, there appears to be a correlation between the presence of sterols with a double bond at the 7-position and neuropathological lesions in young rats.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00684773
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  • 20
    Electronic Resource
    Electronic Resource
    The morphology of experimental tumours of the nervous system (1974)
    Springer
    Acta neuropathologica 27 (1974), S. 25-32 
    Details
    ISSN: 1432-0533
    Keywords: Experimental CNS Tumours ; Nitrosomethylurea ; Ethylnitrosourea ; Primary Cultures ; Tumour Classification ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary By means of the explant technique a series of primary tumours induced by methylnitrosourea (MNU) or ethylnitrosourea (ENU) in the brain of rats were cultured in vitro. The morphology of isomorphic oligodendrogliomas, astrocytomas, glioblastomas and sarcomas under in vitro conditions is described. All these neoplasias show a very distinct morphological picture and growth behaviour, thus permitting their classification. Most of the tumours tend to anaplastic changes during longer cultivation, but in some cases, preferably in well differentiated gliomas, the cytological properties are maintained even during longterm cultivation. The role of anaplasia and intermingling of different neoplastic cell types in experimental tumours are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00687237
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  • 21
    Electronic Resource
    Electronic Resource
    Experimental allergic encephalomyelitis in the rat: Histopathological studies and the effects of sera on adult cerebellar organ cultures (1974)
    Springer
    Acta neuropathologica 27 (1974), S. 83-91 
    Details
    ISSN: 1432-0533
    Keywords: Allergic Encephalomyelitis ; Organ Culture ; Brain ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Experimental allergic encephalomyelitis (EAE) developed in 15 of 30 rats injected with a mixture of Freund's adjuvant and homogenized guinea pig brain, and killed after 6–29 days. The incidence and severity of the disease increased with time after inoculation and in 2 animals involvement of the epiphysis, not previously reported in EAE, was detected. Toxic effects on cells in cultured adult rat cerebellar cortical tissue were produced by exposure for 24 h to the sera of rats killed 12–29 days after inoculation either with the antigenic suspension or with adjuvant alone. Severe toxicity to axons (widespread fragmentation) was produced only by the sera of animals injected with antigenic inocula and killed 17–29 days later. Severe axonal toxicity was not, however, correlated with the presence of EAE in the rats from which the sera were taken. It was not possible to assess myelinotoxic effects in the system used. It is suggested that the organ culture technique may detect the presence in the sera of inoculated animals of toxic principles which, owing to the blood-brain barrier, do not normally contribute to the pathogenesis of EAE.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00687243
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  • 22
    Electronic Resource
    Electronic Resource
    Ultrastructural alterations of rat brain microglial cells and pericytes after chronic lead poisoning (1974)
    Springer
    Acta neuropathologica 28 (1974), S. 25-35 
    Details
    ISSN: 1432-0533
    Keywords: Chronic Lead Intoxication ; Rat ; Ultrastructure ; Microglia ; Pericytes ; Monocytes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Wistar rats of both sexes and the same litter were exposed to chronic lead intoxication from birth untill sacrifice 9 months later. Lead was administered as 0.4% solution of lead nitrate in drinking water. Samples from the parietal brain cortex were examined electron microscopically following intracardiac perfusion with paraformaldehyde-glutaraldehyde solution. Similar changes were observed in the microglial cells and the vascular pericytes whereas all the other tissue elements appeared intact. Both cell types hypertrophied, the microglia assumed characteristic spindle or rod shape, the cell organelles increased, the microglial endoplasmic reticulum widened strongly and a large number of lipid inclusions appeared. The latter consisted of large lipid droplets of varying size and shape, containing multiple zones of low density, and a dense component with a coarse granular structure. The similarity in the response of both cell entities to the lesion as well as some probable functions of microglial cells and their relationship to vascular pericytes are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00687515
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  • 23
    Electronic Resource
    Electronic Resource
    The effects of sodium fluoride on bone breaking strength (1974)
    Springer
    Calcified tissue international 14 (1974), S. 283-289 
    Details
    ISSN: 1432-0827
    Keywords: Sodium fluoride ; Osteoporosis ; Bone ; Strength ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Description / Table of Contents: Résumé L'utilisation thérapeutique de fluorure de sodium a été recommandée dans le traitement de diverses maladies osseuses ostéopéniques. Cet emploi est basé principalement sur les effects ostéosclérotiques bien connus du fluorure de sodium, mais on connait mal son effet sur la force de résistance osseuse. L'influence de diverses concentrations de fluorure de sodium sur la force de résistance osseuse de jeunes rats soumis à des régimes riches et pauvres en calcium a été étudiée. L'administration de fluorure de sodium augmente le diamètre osseux, indiquant une stimulation de la formation de l'os périosté, mais la force de résistance osseuse est réduite ou non modifiée par l'ingestion de fluor.
    Abstract: Zusammenfassung Die therapeutische Verwendung von Natriumfluorid ist für eine Anzahl von Knochenmangel-Krankheiten empfohlen worden. Die Empfehlungen basieren hauptsächlich auf den bekannten osteosklerotischen Wirkungen von Natriumfluorid; über dessen Effekt auf die Knochenstärke ist wenig bekannt. In dieser Arbeit wurde der Einfluß verschiedener Konzentrationen von Natriumfluorid auf die Knochenstärken von wachsenden Ratten mit hoher und niederer Calciumeinnahme untersucht. Die Verabreichung von Natriumfluorid erhöhte den Knochendurchmesser, was auf eine Stimulierung der Periostbildung hinwies, die Knochenstärke wurde jedoch durch Fluorideinnahme herabgesetzt oder nicht beeinflußt.
    Notes: Abstract The therapeutic use of sodium fluoride has been recommended in a variety of osteopenic bone diseases. The recommendations are based mainly on the known osteosclerotic effects of sodium fluoride and little information is available as to its effect on bone strength. The influence of various concentrations of sodium fluoride on bone strength in growing rats on high and low calcium diets was studied. The administration of sodium fluoride increased bone diameter, indicating stimulation of periosteal bone formation, but bone strength was reduced or not affected by fluoride ingestion.
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    URL: http://dx.doi.org/10.1007/BF02060302
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  • 24
    Electronic Resource
    Electronic Resource
    Special aspects of firing rates and thermosensitivity of preoptic neurons (1974)
    Springer
    Experimental brain research 21 (1974), S. 107-112 
    Details
    ISSN: 1432-1106
    Keywords: Preoptic units ; Thermosensitivity ; Mean firing rate ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Mean firing rates (per 10 sec) of preoptic neurons of the rat were recorded at normal temperature and after central warming and cooling. Thermosensitivity was determined according to the reactions of the mean firing rate. In compatibility with results from other animals we obtained the following percentages (52 neurons): 23% warm-sensitive, 13% cold-sensitive, 64% non-thermosensitive neurons. However, it turned out that a lot of interesting special effects are not taken into account, using exclusively the criterion “increasing/decreasing/constant mean firing rate”. Particularly we observed long lasting adaption processes and stationary non-linear effects as well as striking oscillations which were dependent on the thermal stimuli, while the mean interval value remained constant.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00234261
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  • 25
    Electronic Resource
    Electronic Resource
    The contribution of solvent drag to the intestinal absorption of the basic drugs amidopyrine and antipyrine from the jejunum of the rat (1974)
    Springer
    Naunyn-Schmiedeberg's archives of pharmacology 281 (1974), S. 175-196 
    Details
    ISSN: 1432-1912
    Keywords: Intestinal Absorption ; Basic Drugs ; Amidopyrine ; Antipyrine ; Solvent Drag ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary 1. Jejunal loops of anaesthetized rats were perfused with hypotonic, isotonic and hypertonic buffered solutions containing 14C-labelled amidopyrine and antipyrine at neutral and at acidic pH. The blood flow of the loop was maintained at an intermediate rate (0.7–1.0 ml min−1g−1). The water net flux was determined by means of polyethylene glycol as non-absorbable marker and amounted up to ±30 μl min−1g−1. 2. A positive water net flux (directed towards the blood) increased the appearance rate of amidopyrine and antipyrine by maximally 43.8 and 49.2%, a negative water net flux (directed towards the gut lumen) diminished it by 38.8 and 35.0%. 3. The experimental data were analysed by means of a kinetic model with the water net flux as independent variable and the epithelial permeability kF D , the serosal permeability k S F S and the sieving coefficient Φ=1−σ as absorption parameters. For antipyrine independent of the pH-value of the perfusion solution kF D was 0.123, and, for amidopyrine at pH 7 and pH 3 kF D was 0.231 and 0.091, respectively. k S F S was zero indicating that in this experimental arrangement the transfer of drug molecules to the serosal side was negligible. The sieving coefficient Φ amounted to 2.30 for amidopyrine and 2.15 for antipyrine at neutral pH. At acidic pH it amounted to 0.50 for amidopyrine and 1.44 for antipyrine. The hydraulic permeability of water was identical at neutral and acidic pH. 4. The high sieving coefficient for the two drugs at neutral pH is interpreted as indicating that water and lipophilic drug molecules interact within the lipid part of the cell membrane. At acidic pH the ionized drug molecules appear to permeate the cell membrane preferentially across other (presumably more hydrophilic) areas.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00503497
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  • 26
    Electronic Resource
    Electronic Resource
    The influence of blood flow on the phlorizine-insensitive and sensitive galactose absorption in rat jejunum (1974)
    Springer
    Naunyn-Schmiedeberg's archives of pharmacology 282 (1974), S. 195-212 
    Details
    ISSN: 1432-1912
    Keywords: Intestinal Blood Flow ; Intestinal Absorption ; d-Galactose ; Phlorizine ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary 1. Jejunal loops of anaesthetized rats were perfused with buffered solutions (pH 6.8) containing d-galactose in two concentrations: below (100 μM) and above (100 mM) blood sugar concentration. The blood flow rate, the appearance rate (in the intestinal venous blood) and the disappearance rate (from the intestinal lumen) were determined simultaneously, the accumulation in gut tissue at the end of the experiments. 2. At constant intermediate blood flow the appearance rate and disappearance rate decreased with time. A decrease of the blood flow rate from about 1.9 to about 0.3 ml min−1 g−1 diminished both the appearance and the disappearance rate. An increase of blood flow from about 0.3 to about 1.9 ml min−1 g−1 did not increase the absorption rates; even a slight decrease was observed. 3. In the presence of phlorizine (2 mM) the absorption rates and the tissue accumulation of galactose is reduced. At constant intermediate blood flow the appearance and disappearance rate increased with time. A decrease of the blood flow rate from about 1.8 to about 0.3 ml min−1 g−1 diminished the appearance rate. An increase of the blood flow rate from about 0.3 to about 1.8 ml min−1 g−1 caused an increase of the appearance rate in contrast to the experiments without phlorizine. Therefore, the different blood flow dependence of the intestinal absorption observed with actively transported substances can be attributed to their non-passive component.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00499034
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    Electronic Resource
    Fenfluramine-induced ultrastructural alterations in tissues of rats and guinea pigs (1974)
    Springer
    Naunyn-Schmiedeberg's archives of pharmacology 285 (1974), S. 175-184 
    Details
    ISSN: 1432-1912
    Keywords: Fenfluramine ; Rat ; Guinea Pig ; Phospholipidosis, drug-induced ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary After administration of the anorectic drug fenfluramine (40 mg/kg per day) to rats (s. c. or i. p.) and guinea pigs (p.o.) over periods of 2 to 11 weeks, electron microscopic examination was performed on lungs, liver, lymphatic tissues and peripheral blood of both species, and on adrenal glands and ovaries of rats. In rats, fenfluramine caused the following alterations (listed according to the duration of treatment): Formation of abnormal lamellated inclusions in lymphocytes and plasma cells of lymphatic tissues, appearance of “foam cells” in lung alveoli; formation of abnormal lamellated inclusions in corpus luteum, lymphocytes of peripheral blood, and in adrenal cortex. In guinea pigs, the same alterations were found in lymphocytes of spleen and peripheral blood, in hepatocytes and in lung. The present observations support the concept of a generalized phospholipidosis induced by amphiphilic compounds. The potency of fenfluramine to induce a lipidosis is, however, considerably less pronounced than that previously demonstrated for the anorectic drug chlorphentermine. This difference is suggested to be due mainly to the lower degree of amphiphilia of fenfluramine.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00501152
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  • 28
    Electronic Resource
    Electronic Resource
    The action of chlorpromazine on exploration in pairs of rats (1974)
    Springer
    Psychopharmacology 37 (1974), S. 249-254 
    Details
    ISSN: 1432-2072
    Keywords: Chlorpromazine ; Rat ; Social Interaction ; Exploration ; General Motor Activity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract When two animals were placed in a hole-board the number of headdips made by each rat was twice the number made when tested alone, but was the same for pairs of drugged (2 mg/kg chlorpromazine) and undrugged rats, and for pairs where only one rat was drugged. This contrasts with the reduction of headdipping produced by chlorpromazine in single animals. However, chlorpromazine still reduced general motor activity, regardless of whether the partner was drugged or not. The activity of the undrugged rats was affected by the partner's state and was lower when the partner was drugged.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00421538
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  • 29
    Electronic Resource
    Electronic Resource
    Effects of low and high doses of l-Dopa on the tetrabenazine or α-methyltyrosine-induced suppression of behaviour in a successive discrimination task (1974)
    Springer
    Psychopharmacology 39 (1974), S. 199-212 
    Details
    ISSN: 1432-2072
    Keywords: Rat ; Successive Discrimination ; Conditioned Avoidance Response ; Nerve Impulses ; α-Methyltyrosine ; Tetrabenazine ; l-Dopa
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The effect of the administration of l-Dopa, 10 or 100 mg/kg i.p., on the α-methyltyrosine methylester HCl (α-MT; 250+50 mg/kg i. p.) or tetrabenazine (TBZ; 2 mg/kg i.p.)-induced suppression of a successive discrimination-conditioned avoidance task has been studied. It was found that administration of the high l-Dopa dose resulted in a reversal of the α-MT- or TBZ-induced suppression of the avoidance behaviour, although the discrimination was lost. Restoration of the avoidance behaviour and the correct discrimination was obtained with the low l-Dopa dose after α-MT but not after TBZ. The difference in the behavioural response to l-Dopa after α-MT as compared to the response obtained after TBZ is discussed in terms of the availability of the catecholamines formed from l-Dopa for release by the nerve impulses. Biochemical determinations of brain noradrenaline and dopamine were made in parallel. Further, a simple and reliable method for the aquisition of a successive discrimination in the rat is described.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00421027
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  • 30
    Electronic Resource
    Electronic Resource
    Action of a new psychotropic drug (Sulpiride) on avoidance behavior in rats (1974)
    Springer
    Psychopharmacology 39 (1974), S. 309-314 
    Details
    ISSN: 1432-2072
    Keywords: Avoidance ; Drug ; Psychotropic ; Rat ; Sidman Schedule ; Sulpiride
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Sulpiride, a new psychotropic drug with neuroleptic activity, was administered to rats in a Sidman avoidance schedule. Doses ranging from 1 to 80 mg/kg resulted in the animal receiving fewer shocks. This effect was not correlated with an increase of responding and seemed unrelated to the suppressing action abruptly observed at high doses (i. e. 150 to 200 mg/kg). These results differ from those classicaly obtained with other neuroleptics.
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    URL: http://dx.doi.org/10.1007/BF00422970
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  • 31
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    Electronic Resource
    Behavioural and biochemical effects of 6-hydroxydopa in rats (1974)
    Springer
    Psychopharmacology 35 (1974), S. 341-352 
    Details
    ISSN: 1432-2072
    Keywords: 6-Hydroxydopa ; Rat ; Behaviour ; Biogenic Amines
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The influence of 6-hydroxydopa (6-HDP) injected into the lateral ventricles of rat brain on the behaviour of animals was examined. The level of noradrenaline (NA), 5-hydroxytryptamine (5-HT) and 5-hydroxyindoleacetic acid (5-HIAA) in discrete areas of the brain was also measured. 6-HDP was injected in a dose of 100 or 150 Μg into both lateral ventricles, or in a dose of 200 Μg into the right lateral ventricle. Immediately after 6-HDP injection, circling movements, convulsions, aggressive behaviour, and Stereotypic activity were seen. These components of behaviour were most obvious during the first hour after injection of 6-HDP. During one month after 6-HDP administration the behaviour of rats did not differ significantly from the behaviour of control animals, only very subtle differences in behaviour being seen. The dose of 150 Μg of 6-HDP caused aphagia and loss of body weigth during the first 5 days after treatment. 6-HDP also caused hypothermia. 20 min after administration of 200 Μg of 6-HDP a decrease of the NA level but no changes in the 5-HT level in the brain cortex were seen. The same changes were observed 1 month after 6-HDP treatment. The dose of 150 Μg of 6-HDP decreased the NA level in the brain cortex, cerebellum, mesencephalon and brain stem 5 days after treatment. 5-HT content was not changed and the 5-HIAA level was increased in the same brain areas. The dose of 100 Μg of 6-HDP, 2 weeks after the treatment decreased the content of NA in the brain cortex, cerebellum, hypothalamus and brain stem without changes in 5-HT content. 5-HIAA level was elevated only in the brain stem. It is concluded that 6-HDP is a long-acting potent depletor of NA in rat brain. There is a different sensitivity of brain areas to the depleting effect of 6-HDP. 6-HDP does not deplete the 5-HT content of discrete areas of brain but increases the level of 5-HIAA. 6-HDP causes distinct behavioural changes shortly after the treatment. It does not change the behaviour of rats between 1 and 30 days after its administration.
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    URL: http://dx.doi.org/10.1007/BF00429225
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  • 32
    Electronic Resource
    Electronic Resource
    Conditioned aversion in spatial paradigms following methamphetamine injection (1974)
    Springer
    Psychopharmacology 36 (1974), S. 323-335 
    Details
    ISSN: 1432-2072
    Keywords: Methamphetamine ; Rat ; Conditioned ; Aversion ; Space ; Saccharin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Three experiments were performed on Sprague-Dawley derived rats in which 3.0 mg/kg, i.p., injections of methamphetamine were paired with either, (1) a distinctive spatial enclosure, i.e., either a black or gray portion of a shuttle box with discriminably different flooring, (2) the enclosure plus 0.1% saccharin solution availability in the home cage, or (3) the enclosure plus 0.1% saccharin solution availability in that area. Following three drug pairings which alternated with saline injections paired with a different enclosure or enclosure plus H2O availability, a choice of either spatial (1), or spatial plus gustatory stimuli (2,3) which included the original CS's, were presented under non-drug, non-injection conditions. Control groups received saline injections each day under identical conditions. No aversion to the enclosed area, which had been paired with the drug was demonstrated under (1), gustatory (P=0.005) but not spatial aversion was in evidence in the second paradigm, and spatial (P=0.01) and gustatory (P〈0.01) aversion was exhibited in the last study. It was concluded that subsequent aversion to distinctive spatial enclosures following methamphetamine injections was not as strong an effect as similar aversions to gustatory stimuli. Furthermore, the effect only obtained to neutral and not to preferred spatial areas at the dose level tested and in the apparatus used in those studies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00422564
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  • 33
    Electronic Resource
    Electronic Resource
    In vivo studies on uricase activity in the rat (1974)
    Springer
    Pflügers Archiv 351 (1974), S. 323-330 
    Details
    ISSN: 1432-2013
    Keywords: Uricase ; Urate ; Allantoin ; Liver ; Kidney ; Microperfusion ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary 1. In vivo uricase activity was tested in rats by injection of 2-C14 urate and measurement of the total C14 activity and the fractional activities of allantoin, allantoic acid and urea in samples of blood and urine. In control animals, 5 min after the injection, 70% of the plasma tracer was already present in the form of allantoin. No allantoic acid and urea were produced. Intestinectomy had no measurable influence on uricase activity. On the other hand, hepatectomy or ligation of the hepatic artery combined with subtotal viscerectomy did abolish uricase activity almost completely. 2. Following microinjections into proximal tubules of Ringer solution containing 2-C14 urate, urine samples during early recovery mainly contained labelled urate, whereas in later samples the fraction of labelled allantoin increased. About 12 min after the microinjection the urine of both kidneys contained equal amounts of tracer mainly in the form of allantoin. 3. When segments of proximal tubules were perfused with an equilibrium solution containing tracer amounts of C 14 urate, no urate was metabolized during its passage through the proximal tubule. 4. C 14 urate was offered from the peritubular capillaries and samples of tubular fluid were analyzed, Again, all the tracer in the tubular fluid was in the form of urate, indicating that urate is not oxidized when it is transported across the tubular cell. It is concluded from these results that: 1. The rat kidney has no significant uricase activity. 2. Urate transport in the kidney is not influenced by this enzyme. 3. The degradation of urate to allantoin takes place at extrarenal sites, mainly in the liver.
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    URL: http://dx.doi.org/10.1007/BF00593318
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  • 34
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    Electronic Resource
    Cardiac hypertrophy in the first generation of rats native to simulated high altitude (1974)
    Springer
    Pflügers Archiv 350 (1974), S. 241-248 
    Details
    ISSN: 1432-2013
    Keywords: Cardiac Hypertrophy ; Diffusion Distance of Oxygen ; High Altitude ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In rats born and staying their whole life at a simulated altitude of 3500 m a severe cardiac hypertrophy develops which is most pronounced in the right ventricle. On histological sections muscle fiber and capillary densities were counted and the surfaces occupied by muscle fibers and capillaries in the slides were estimated. Mean muscle fiber diameter and half mean distance between capillaries (diffusion distance) were obtained in the heart ventricles and were compared with those of control animals. In both ventricles an increase of the mean muscle fiber diameter occurred (true hypertrophy), being more pronounced in the right ventricle. We cannot tell whether also hyperplasia of muscle fibers occurred or not. The diffusion distance in the left ventricle of rats exposed to hypoxia was about the same as in control animals but was shorter in the right ventricle. This indicates that the total number of capillaries is higher in both ventricles of rats born in the low pressure chamber (hyperplasia of the capillaries). The shortening of the diffusion distance in the right ventricle of rats born and living their whole life at simulated high altitude was on the average smaller than that of rats exposed to simulated high altitude later in life. The physiological importance of this observation is discussed.
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    URL: http://dx.doi.org/10.1007/BF00587803
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  • 35
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    Electronic Resource
    Analysis of periodic components of hypothalamic spike-trains after central thermal stimulation (1974)
    Springer
    Pflügers Archiv 351 (1974), S. 13-24 
    Details
    ISSN: 1432-2013
    Keywords: Hypothalamus ; Spike-Trains ; Correlogram/Periodogram ; Thermosensitivity ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Stationary spike-trains of preoptic neurons of the rat were submitted to a correlation- and spectral-analysis at normal temperature and after central warming and cooling. Correlograms and periodograms were computed which enable the elimination of stochastic signal components and the identification of the relevant periodic components. About one half of the analysed neurons showed periodic correlograms. The following three classes may be distinguished: Neurons which showed correlograms with periodicities at each applied thermal state, at only one or two thermal states and those with no recognizable periodicities at all. Relations between periodicity and the thermosensitivity of the neurons are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00603507
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  • 36
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    Demonstration by degeneration silver method of the ascending projection from the locus ceruleus (1974)
    Springer
    Experimental brain research 20 (1974), S. 181-192 
    Details
    ISSN: 1432-1106
    Keywords: Locus ceruleus ; Cerulo-cortical tract ; Ascending reticular fiber ; Rat ; Fink-Heimer method
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In order to demonstrate the ascending projection from the locus ceruleus by degeneration silver method, the nucleus was destroyed unilaterally in rats and the brains were stained by a modified Fink-Heimer method following various survival times. Additional experiments included the bilateral destruction of the locus ceruleus and the administration of 6-OH-DOPA. For the demonstration of degenerated terminals in the cerebral cortex it is essential to choose an optimal survival time of 4 or 5 days, keep the time of fixation below 1 week, and perform treatments with permanganate and with silver at 37°C. The trajectory and distribution of the cerulo-cortical tract as revealed by the silver degeneration methods is in agreement with the results of the histofluorescence method, with some minor differences. The ascending fibers run through Forel's tegmental fascicle, turn rostro-ventrally to the subthalamus, enter and cross the internal capsule, pass through the ventral part of the caudate-putamen and distribute to the entire cerebral cortex. The tract is mainly ipsilateral, though some fibers are crossing.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00234012
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  • 37
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    Electronic Resource
    Comparative study of the absorption, elimination and acute hepatotoxic action of ethanol in guinea pigs and rats (1974)
    Springer
    Archives of toxicology 32 (1974), S. 83-95 
    Details
    ISSN: 1432-0738
    Keywords: Alcohol, Ethyl ; Absorption ; Elimination ; Alcohol Oxidoreductases ; Hepatitis, Toxic ; Transaminases ; Glutamate Dehydrogenase ; Guinea Pig ; Rat ; Äthylalkohol ; Resorption ; Elimination ; Alkoholdehydrogenase ; Hepatotoxicität ; Transaminasen ; Glutamatdehydrogenase ; Meerschweinchen ; Ratte
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Die maximalen Blutalkoholkonzentrationen nach oraler Gabe von 6,4 g/kg Äthanol in Form einer 40%igen Lösung (v/v) betrugen bei nicht nüchternen Meerschweinchen 6,8±0,3 mg/ml, bei nicht nüchternen Ratten dagegen nur 2,1±0,2 mg/ml. An nüchternen Tieren führte eine orale Alkoholbelastung von 4,8 g/kg zu maximalen Blutalkoholspiegeln von 6,3±0,2 mg/ml bei den Meerschweinchen und 3,7±0,3 mg/ml bei den Ratten. Intravenös injizierter Alkohol (1 g/kg) wurde demgegenüber von beiden Species mit der gleichen Geschwindigkeit eliminiert (275 mg/kg · Std), und die auf das Körpergewicht bezogene ADH-Aktivität der Leber war bei den Meerschweinchen um 20% größer als bei den Ratten. Die Resorption von Äthanol erfolgt somit bei Ratten erheblich langsamer als bei Meerschweinchen. Dies dürfte darauf beruhen, daß konzentrierte Alkohollösungen die Magenentleerung der Ratte stark verzögern. Niedriger konzentrierte Alkohollösungen werden von der Ratte schneller resorbiert; jedoch waren auch nach Gabe von 10%igem Alkohol die maximalen Blutalkoholspiegel bei Ratten noch um 36% niedriger als bei Meerschweinchen. Bei den Meerschweinchen waren nach oraler Gabe von 4,8 bzw. 6,4 g/kg Äthanol die Serumaktivitäten der GOT, GPT und GLDH erhöht; ein Anstieg der SGOT trat schon nach 1,6 g/kg Äthanol auf. Demgegenüber kam es bei Ratten nach 6,4 g pro kg Äthanol p.o. nur zu geringfügigen Aktivitätserhöhungen der Serumtransaminasen, während die Serumaktivität der GLDH unverändert blieb. Vacuolige Degeneration war das morphologische Substrat der durch Äthanol bei Meerschweinchen und Ratten ausgelösten Leberschädigung. Während diese Veränderung bei Meerschweinchen bereits nach einer Äthanoldosis von 1,6 g/kg auftrat, wurde sie bei Ratten erst nach 6,4 g/kg beobachtet. Schlußfolgerung: Das Meerschweinchen ist für Untersuchungen zur Toxicität von Äthanol besser geeignet als die Ratte.
    Notes: Abstract In fed guinea pigs, an oral dose of 6.4 g/kg of ethanol given as a 40% solution (v/v) produced a maximal blood alcohol level of 6.8±0.3 mg/ml, whereas in fed rats, blood alcohol levels after the same dose did not exceed 2.1±0.2 mg/ml. Maximal blood alcohol levels in fasted animals after an oral load of 4.8 g/kg of ethanol were 6.3±0.2 mg/ml in guinea pigs and 3.7±0.3 mg/ml for rats. However, i.v. injected ethanol (1 g/kg) was eliminated at the same rate in both species (275 mg per kg · h), and ADH activity of the liver related to body weight was by 20% greater in guinea pigs than in rats. Therefore, absorption of ethanol occurs at a much slower rate in rats than in guinea pigs. This is possibly due to the fact that high ethanol concentrations strongly delay emptying of the rat stomach. Lowering the ethanol concentration accelerates absorption rate in the rat. However, even after gavage of a 10% solution peak levels of blood alcohol were still lower by 36% in rats than in guinea pigs. In guinea pigs, increased serum activities of GOT, GPT, and GLDH occurred after an oral dose of 4.8 g/kg or 6.4 g/kg of ethanol, respectively. SGOT already increased after 1.6 g/kg of ethanol p.o. After 6.4 g/kg of ethanol given to rats serum transaminase levels increased only slightly, and GLDH activity not at all. Vacuolar degeneration was the morphological substrate of ethanol-induced liver damage in guinea pigs and rats. In guinea-pigs, it occurred already after 1.6 g/kg of ethanol, whereas in rats only after 6.4 g/kg. In conclusion, the guinea pig seems to be better suited for research on alcohol toxicity than the rat.
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    URL: http://dx.doi.org/10.1007/BF00316229
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  • 38
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    Resistance to extinction of an avoidance response in rats following the administration of chlordiazepoxide (Librium) or diazepam (Valium) (1974)
    Springer
    Psychopharmacology 38 (1974), S. 231-238 
    Details
    ISSN: 1432-2072
    Keywords: Rat ; Chlordiazepoxide ; Diazepam ; Extinction ; Avoidance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract In a one-way avoidance task with rats, injections of Librium (chlordiazepoxide) following avoidance acquisition resulted in prolonged resistance-to-extinction of the avoidance response. This effect occurred regardless of whether the rats had had prior experience with Librium or whether they were naive with respect to the drug. The same results were found with the same task when low doses of Valium were used. However, at a higher dosage an “extreme reaction” of either no responding or a high number of responses to extinction occurred in the naive animals. The Librium and Valium effects were compared to similar effects obtained using ethanol and hashish resin. These results indicate that the novelty hypothesis as originally stated by Amit and Baum cannot be supported because experience with the drugs prior to avoidance training did not attenuate the drug effect on avoidance.
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    URL: http://dx.doi.org/10.1007/BF00421375
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  • 39
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    Effect of LSD-25 and 1-methyl-d-lysergic acid butanolamide on rat brain and platelet serotonin levels (1974)
    Springer
    Psychopharmacology 36 (1974), S. 117-122 
    Details
    ISSN: 1432-2072
    Keywords: LSD-25 ; UML ; 5-HT ; Brain ; Platelets ; Behaviour ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Factorial analysis of rat cerebral 5-HT values observed following intraperitoneal LSD-25 and UML (1-methyl-d-lysergic acid butanolamide) showed that the highly significant decrease induced by both drugs was independent of dose and that the effect of both drugs was the same. Increased platelet 5-HT values were also obtained with each drug. These findings suggest that the psychotomimetic effect of LSD-25 is not due to changes in cerebral 5-HT, since a relationship can probably be postulated between such an effect in man and the effect of LSD-25 on conditioned behaviour in the rat. It is also felt that the fact that these drugs decrease and increase cerebral and platelet values respectively may be of assistance in the interpretation of data relating to platelet 5-HT patterns in man under psychiatric conditions and during treatment with psychotropic drugs.
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    URL: http://dx.doi.org/10.1007/BF00421783
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  • 40
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    Effect of acute and chronic ethanol administration on serotonin turnover in rat brain (1974)
    Springer
    Psychopharmacology 37 (1974), S. 91-100 
    Details
    ISSN: 1432-2072
    Keywords: Ethanol ; Brain Serotonin Turnover ; Tolerance ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract In rats treated acutely and chronically with ethanol, brain serotonin (5-HT) turnover was determined by measuring in the same animals the rate of accumulation of 5-HT and the rate of decline of 5-hydroxyindoleacetic acid (5-HIAA) in the brain after administration of the MAO inhibitor pargyline-HCl. In the acute study, 5-HT turnover was not altered after a single 4 g/kg oral dose of ethanol. In two separate studies, it was shown that chronic administration of ethanol in the form of a liquid diet for 32 days also did not alter brain 5-HT turnover, even when the rats had developed a significant degree of tolerance to the motor-impairing effects of ethanol. These results suggest that tolerance to ethanol is unrelated to any change in brain 5-HT turnover.
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    URL: http://dx.doi.org/10.1007/BF00437416
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  • 41
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    Electronic Resource
    Urate handling by the rat kidney (1974)
    Springer
    Pflügers Archiv 352 (1974), S. 115-120 
    Details
    ISSN: 1432-2013
    Keywords: Urate ; Reabsorption ; Loop of Henle ; Micropuncture ; Microperfusion ; Microinjection ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Reabsorption rates for urate in the loops of Henle were measured in superficial nephrons in the rat 1. under conditions of free flow, 2. using microperfusion and 3. by a microinjection technique. 1. Under conditions of free flow distally measured TF/PUA/TF/PIn-values varied between 0.51 and 0.38 in antidiuretic rats, depending on TF/PIn (UA = both uric acid and urate, In = inulin, TF/P = concentration in tubular fluid to plasma concentration). The corresponding values in samples from end-proximal tubules were 1.06 and in urine 0.19 (U/PUA/U/PIn). 2. In microperfusion experiments of Henle loops early distal recoveries of 2-C14 urate varied between 57 and 86%, depending on the flow rates (10–40 nl/min). 3. In microinjection experiments C14 recovery in urine was about 85% when tracer solution was microinjected into endproximal tubules. From these results we conclude: 1. The main site of urate reabsorption is located in the loops of Henle. 2. This reabsorption is highly dependent on flow rates. Increase of flow rate through Henle's loop decreases urate reabsorption.
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    URL: http://dx.doi.org/10.1007/BF00587510
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  • 42
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    Urate interaction with plasma proteins and erythrocytes (1974)
    Springer
    Pflügers Archiv 352 (1974), S. 121-133 
    Details
    ISSN: 1432-2013
    Keywords: Urate ; Protein Interaction ; Uptake by Erythrocytes ; Renal Reabsorption ; Man ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Interaction of urate with human and rat plasma was studied by a dialysis technique at different temperatures. At 4° C a certain fraction of urate is bound to proteins. However, this fraction diminishes with increasing temperature and at 37° C only some 7–8% (in man) and 2% (in rat) interact with proteins. The finding that urate is almost completely filtered in the glomerulus is discussed. In body areas exposed to low temperatures protein binding of urate may be of importance. Urate uptake by erythrocytes is characterized by two components: a fast component equilibrating almost immediately at about 7% in man and 17% in rat and a slow component reaching equilibrium after 60 min, at 28% and 36%, respectively. The process is described by a mathematical formula. Lowering of the temperature mainly diminishes uptake by the slow component withQ 10-values ranging between 1.5 and 4.0. In the observed range of concentrations the uptake process does not saturate. The results at lower pH-values suggest that it is unionized uric acid which is transported by the slow component. Application of the data to kidney medulla supports the hypothesis that erythrocytes and, probably, to a lesser extent plasma proteins serve as vehicles for urate reabsorption in the countercurrent system. Such a temporary interaction enables uric acid to escape recirculation and to leave the kidney medulla.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00587511
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    The effects of scopolamine and physostigmine on fixed-interval behaviour in the rat (1974)
    Springer
    Psychopharmacology 39 (1974), S. 237-244 
    Details
    ISSN: 1432-2072
    Keywords: Antagonism ; Fixed Interval ; Scopolamine ; Physostigmine ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The purpose of this research was to determine if 2 drugs, scopolamine and physostigmine which have opposite effects on cholinergic transmission also produce opposite effects on fixed-interval (FI) performance. Scopolamine and a wide range of doses of physostigmine were administered singly and concurrently to a number of rats responding on a FI 2 min schedule. Scopolamine produced a disruption in FI curvature which could be antagonized by physostigmine, but physostigmine by itself produced no consistent change.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00421031
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  • 44
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    Electronic Resource
    The role of sex and novelty in determining the social response to lithium chloride (1974)
    Springer
    Psychopharmacology 40 (1974), S. 91-99 
    Details
    ISSN: 1432-2072
    Keywords: Lithium ; Social Behaviour ; Sex ; Novelty ; Photographs ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Effects of lithium chloride on the social behaviour of male and female rats were measured in unfamiliar and familiar test situations using a photographic technique. Lithium-treated females showed an increase in body contact and a decrease in social distances in comparison with saline-treated controls in both test conditions. Males administered lithium showed increased corner occupancies in the novel test setting while in the familiar test they showed decreased body contact and increased average group social distance in comparison with the saline condition. Further tests in an exploration box confirmed that lithium decreased the general environmental responsiveness of male rats. The results are discussed with reference to the anti-aggressive effect of lithium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00429450
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  • 45
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    In vitro analysis of the cellular localization of luteinizing hormone releasing factor (LRF) in the basal hypothalamus of the rat (1974)
    Springer
    Cell & tissue research 149 (1974), S. 371-378 
    Details
    ISSN: 1432-0878
    Keywords: LRF ; Hypothalamic localization ; Rat ; Ependyma ; Glia ; Light and electron microscopic Autoradiography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Light and electron microscopic autoradiographic analysis of the medial basal hypothalamus of rat brains incubated for 30 and 60 minutes in tritiated luteinizing releasing factor (3H-LRF) revealed selective uptake in the form of silver grains over specialized ependyma (tanycytes) and perivascular glial cells in the palisade-contact zone of the median eminence. The functional capacity of tanycytes and glia are discussed in light of these data.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00226771
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  • 46
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    Electronic Resource
    Failure to demonstrate serotonergic neurons in the myenteric plexus of the rat (1974)
    Springer
    Cell & tissue research 150 (1974), S. 493-496 
    Details
    ISSN: 1432-0878
    Keywords: Serotonin ; Neurotransmitter ; Myenteric plexus ; Rat ; Histofluorescence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Several recent studies suggested that serotonergic neuron-like elements are present in the guinea pig ileum. The present paper reports an extensive study of the digestive tract of the rat with the use of a histofluorescence technique. Administration of the serotonin precursor, tryptophan, associated with a monoamine oxidase inhibitor, did not allow histochemical demonstration of rapidly fading, yellow fluorescent, 6-hydroxydopamine-resistant neurons; conversely such neurons were readily detected in the brain. It is concluded that serotonergic neuron-like elements cannot be detected histochemically in the rat myenteric plexus area after chemical sympathectomy.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00225972
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  • 47
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    Electronic Resource
    Studies on the juxtaglomerular apparatus (1974)
    Springer
    Cell & tissue research 151 (1974), S. 457-469 
    Details
    ISSN: 1432-0878
    Keywords: Juxtaglomerular apparatus ; Rat ; Adrenalectomy ; Renin and secretory granules ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Die Reaktion des juxtaglomerulären Apparates auf Adrenalektomie wurde untersucht. Im Stadium der Nebenniereninsuffizienz, sechs Tage nach der Operation, wurde quantitativ morphometrisch eine Abnahme der Volumendichte der Sekretgranula (von 32% auf 16%) festgestellt. Golgi-Apparat und Ergastoplasma proliferieren stark, wobei insbesondere die erweiterten Cisternen des Ergastoplasmas auffallen.
    Notes: Summary The response of the Juxtaglomerular apparatus to bilateral adrenalectomy was studied in rats. After the sixth postoperative day, symptoms of severe adrenal insufficiency had developed. In the Juxtaglomerular cells, the volume density of the secretory granules decreased from 32% to 16%. The Golgi apparatus and rough endoplasmic reticulum underwent considerable proliferation, the latter showing strikingly enlarged cisternae.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222991
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  • 48
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    Electronic Resource
    Sex differences in adrenocortical structure and function (1974)
    Springer
    Cell & tissue research 151 (1974), S. 525-536 
    Details
    ISSN: 1432-0878
    Keywords: Adrenal cortex ; Rat ; Sex differences ; Gonadal hormones ; Karyometry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Adrenal glands of adult female rats are heavier than the glands of corresponding male rats. Postpubertal orchiectomy increases the adrenal weight, an effect restored by testosterone replacement. Under the same conditions ovariectomy of 8 weeks duration does not change the adrenal weight while estradiol replacement enhances the relative adrenal weight. Karyometric studies have shown that nuclei in the female zona fasciculata cells are larger (app. 18%) than those of the male. Similar but only slight differences (2%) were observed in the zona reticularis. Orchiectomy results in enlargement of cell nuclei within all zones of the adrenal cortex; testosterone replacement has the opposite effect. Ovariectomy of 8 weeks duration slightly enhances the volume of nuclei of the zona glomerulosa cells, has no effect on the nuclei of the zona fasciculata and reduces the volume of nuclei in the zona reticularis. Estradiol replacement reduces the volume of nuclei of the zona glomerulosa cells but increases nuclear volume in the zona fasciculata and in the zona reticularis. Thus testosterone has an inhibitory effect on the adrenal cortex of the rat while the physiologic effect of estradiol on the morphology of this gland, particularly on the zona fasciculata cells is rather dubious. The author wishes to thank Mrs. B. Westerska and Miss K. Siejak for excellent technical assistance.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222997
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  • 49
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    Electronic Resource
    Sex differences in adrenocortical structure and function (1974)
    Springer
    Cell & tissue research 151 (1974), S. 537-547 
    Details
    ISSN: 1432-0878
    Keywords: Adrenal cortex ; Rat ; Sex differences ; Gonadal hormone ; Stereologic studies
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Adult female rats have heavier adrenals than males. Orchiectomy increases the adrenal weight, an effect reversed by testosterone. Neither ovariectomy nor estradiol replacement has an effect on absolute adrenal weight. The stereologic analysis of the volume fractions of particular adrenocortical zones have shown that the volume fraction of the zona glomerulosa in females is smaller as compared with males. On the contrary, the volume fractions of the fasciculata and reticularis zones are higher in female than in male rats. In both sexes gonadectomy and gonadal hormone replacement has an effect on the volume fractions of particular adrenocortical zones, these changes being more pronounced due to orchiectomy or testosterone replacement. The adrenal cortex of all experimental groups contains a similar quantity of parenchymal cells. The cytoplasm volume of a single adrenocortical cell is higher in all zones of the female adrenals as compared with the male. Orchiectomy increases the volume of the cytoplasm in fasciculata and reticularis cells. This effect was reversed by testosterone. Neither ovariectomy nor estradiol replacement has an effect on the volume of the cytoplasm in the fasciculata and reticularis cells. These studies have shown that sex differences with regards to rat adrenal size and weight depend upon the “physiologic atrophy” of the male adrenal cortex, while neither ovariectomy (8 weeks after operation) nor estradiol replacement has a conspicuous effect on the morphologic changes of this gland.
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    URL: http://dx.doi.org/10.1007/BF00222998
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  • 50
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    Ultrastructural investigations of the palisade-shaped nerve fiber terminals of the normal hairs of rat's snout (1974)
    Springer
    Cell & tissue research 147 (1974), S. 259-269 
    Details
    ISSN: 1432-0878
    Keywords: Nerve endings ; Hair ; Rat ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Palisade-shaped nerve endings of the small normal hairs of the rat snout were examined with the electron microscope (fixation by perfusion). The terminals are located inside the ‘glassy membrane’ in the area of the neck of the hair root. The 10–20 radially arranged terminal axons are in direct contact with the basement membrane of the epithelium of the external root sheath. The axons are surrounded on all sides by leaf-shaped processes of the Schwann cells. The surfaces of these cell processes are marked by numerous vesicle-like invaginations (approx. 1000 Å dia.). Transverse sections from several areas of the palisadeshaped nerve endings are compared with longitudinal sections. In the upper area ‘empty’ vesicles (approx. 500–600 Å in diameter) occur, along with electron-dense vesicles (approx. 800–1100 Å in diameter); in the middle area, the axons are distended and contain accumulations of mitochondria.
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    URL: http://dx.doi.org/10.1007/BF00582800
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    The parietal layer of bowman's capsule in experimental hypertension (1974)
    Springer
    Cell & tissue research 147 (1974), S. 183-190 
    Details
    ISSN: 1432-0878
    Keywords: Kidney ; Rat ; Bowman's capsule ; Experimental hypertension
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The parietal layer of Bowman's capsule was examined in the rat in control animals and in animals made hypertensive by treatment with desoxycorticosterone acetatesaline and by clipping of the renal artery. At an early stage of elevation of blood pressure and, occasionally, prior to elevation of blood pressure, the epithelial cells of this layer showed basal cytoplasmic protrusion and reduplication accompanied by an increase in the thickness of the basement membrane. These changes preceded evidence of glomerular or renal arteriolar pathology. They presumably reflect either a response of this layer to increased blood pressure and modified glomerular function, or a direct effect of the factors that also induces blood pressure elevation. In either case, they imply a greater functional significance for the parietal layer than has usually been attributed to it.
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    URL: http://dx.doi.org/10.1007/BF00582793
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  • 52
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    The ultrastructure of the accessory sex organs of the male rat (1974)
    Springer
    Cell & tissue research 148 (1974), S. 57-67 
    Details
    ISSN: 1432-0878
    Keywords: Prostate ; Rat ; Cyproterone ; Alterations ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The dorsal lobe and the coagulating gland of the rat prostate were studied after administration of the anti-androgenic compound Cyp A for varying periods (7–20 days). There was a general involution of the organs with macroscopic atrophy and reduced amount of secretory material. Ultrastructurally the cells were pale, contained less organelles as compared with the controls, loss of cytoplasm and reduction both of cell height and width. The major changes also included reduction of the Golgi area, the rough endoplasmic reticulum and the number of free ribosomes. Within the dorsal lobe prominent nuclear changes were found, comprising different types of intranuclear inclusions of membranous configurations. The alterations observed in the present study are similar to the changes which were found in castrated animals and are consistent with atrophy. The results are discussed in relation to the available biochemical and autoradiographic data on the different lobes.
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    URL: http://dx.doi.org/10.1007/BF00224318
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  • 53
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    Electronic Resource
    Ultrastructure of mucosal mast cells in normal and compound 48/80-treated rats (1974)
    Springer
    Cell & tissue research 150 (1974), S. 95-105 
    Details
    ISSN: 1432-0878
    Keywords: Mucosal mast cells ; Rat ; Compound 48/80 ; Degranulation, Release ; Light microscopy ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Mast cells in the tongue and lamina propria of the duodenal mucosa, representing two cell types with different morphological, histochemical and functional properties have been studied under the electron microscope. Both cell types were found to contain similar moderately electron dense and homogeneous granules surrounded by a tight membrane constituting the basic ultrastructural characteristics of mast cells. The mucosal mast cells, however, contained fewer granules per cell and the individual granules often exhibited considerable variation in size. In addition the mucosal mast cells, unlike those of the tongue, peritoneum and skin, had a smooth plasma membrane lacking microvilli and possessed irregularly shaped or lobulated nuclei. In the tongue mast cells, administration of Compound 48/80 caused the formation of vacuoles around the granules, expulsion of granules from the cells and disintegration of the granular matrix, but no changes that could be attributed to 48/80 could be found in the mucosal mast cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00220383
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  • 54
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    Kinetics of gametogenesis (1974)
    Springer
    Cell & tissue research 154 (1974), S. 443-470 
    Details
    ISSN: 1432-0878
    Keywords: Oocytes and T-prospermatogonia ; Rat ; Quantitative analysis ; Autoradiography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Bei der Ratte findet die sexuelle Differenzierung der Gonade zwischen dem 14. und 15. Tag post conceptionem (p.c.) statt. Während dieser Zeit teilen sich die Oogonien und deren Parallelpopulation—die M-prospermatogonien (multiplying prospermatogonia) oder I-Gonocyten—sehr häufig. Um den 17. Tag p.c. tritt die letzte Generation der Oogonien bzw. der M-prospermatogonien in die Mitose. Die Mitosen bilden häufig “synchronisierte Gruppen”. Die postmitotischen Kerne ihrer Tochterzellen—der Oocyten und der T 1 prospermatogonien (primary transitional prospermatogonia) oder II-Gonocyten—sind klein. Ihr Chromatin ist in Form gröberer Schollen der Kernmembran angelagert. Auf diese Weise kommt das typische “krustenförmige” Aussehen bzw. die Ähnlichkeit mit den Prophasen der Oogonien und M-prospermatogonien zustande. Die Oocyten durchlaufen zunächst die G1-phase (etwa 10 Std Dauer) und treten dann—am Ende des Präleptotänstadiums—in die S-phase. Dann passieren sie die verschiedenen Stadien der meiotischen Prophase und treten vom 3. Tag post partum (p.p.) ab in das Dictyotänstadium. Die T1-prospermatogonien hingegen befinden sich etwa 10 Tage lang in der G1-phase, ohne wesentliche morphologische Veränderungen aufzuweisen. Vom 4. Tag p.p. an durchlaufen sie die S-Phase. Die S-Phasen-Dauer (D-S) beider Zellarten beträgt 11.5 Std und wurde durch Doppelmarkierung mit 14C- und 3H-Thymidin bestimmt. Als am besten geeignete Termine für die Bestimmung der D-S erwiesen sich bei den Oocyten der 18. Tag p.c. und bei den T1-prospermatogonien der 5. Tag p.p. Zu diesen beiden Zeitpunkten war die Anzahl der in die S-phase ein-und austretenden Oocyten bzw. T1-prospermatogonien gleich, die Zellen in S-phase befanden sich im “steady state”. Das Kernvolumen der Oogonien und M-prospermatogonien ist etwa doppelt so groß wie das der postmitotischen Oocyten und T1-prospermatogonien. Bis zum 5. Tage p.p. nimmt das Kernvolumen der Oocyten und T1-prospermatogonien um etwa das Fünffache zu. Der Degenerationsindex der Oocyten liegt wesentlich höher als der der T1-prospermatogonien; er ist postnatal besonders hoch. Aus den T1-prospermatogonien gehen am 4. und 5. Tage p.p. durch Teilung die T 2-prospermatogonien (secondary transitional prospermatogonia) hervor. Die Kerne dieses Zelltyps sind etwas kleiner also die der T1-prospermatogonien. Die T2-prospermatogonien treten am 6. Tage p.p. in die Mitose; es entstehen die ersten A-spermatogonien.
    Notes: Summary In the rat (Wistar-WU) sexual differentiation of the gonads occurs between days 14 and 15 post conception (p.c.). At this time the oogonia and their parallel population — the M-prospermatogonia (I-gonocytes)—divide rapidly. On about day 17 p.c., the last generation of oogonia and M-prospermatogonia, frequently arranged in synchronized clusters, enters mitosis. The postmitotic nuclei of their daughter cells—oocytes and T 1-prospermatogonia (II-gonocytes)—are small; coarse flakes of chromatin are associated with the nuclear membrane causing the typical “crustlike” appearance and the similarity with the prophases of oogonia and M-prospermatogonia. After the oocytes have passed a G1-phase of approximately 10 hr, they enter the S-phase at the end of the preleptotene stage. Then they pass the different stages of the meiotic prophase until they enter the dictyate stage from 3 day post partum (p.p.) onwards. The T1-prospermatogonia, on the other hand, spend a long G1-phase of about 10 days without any conspicuous morphological change before entering the S-phase from day 4 p.p. onwards. The duration of the S-pbase (D-S) of both cell types—oocytes and T1-prospermatogonia—as determined by the double labeling method with 14C- and 3H-thymidine is found to be 11.5 hr. The most favourable time for determining the D-S was day 18 p.c. for the oocytes and day 5 p.p. for the T1-prospermatogonia. On these two days the balance was reached between the cells entering and leaving the S-phase. The nuclear volumes of the postmitotic oocytes and T1-prospermatogonia are approximately half the size of those of their precursors. Until day 5 p.p. the nuclear volumes of the oocytes and T1-prospermatogonia increase about fivefold. The degeneration index of the oocytes is considerably higher than that of the T1-prospermatogonia; postnatally it is especially high. T 2 prospermatogonia arise by mitosis of the T1-prospermatogonia on day 4 and 5 p.p. The nuclei of this cell type are smaller than those of T1-prospermatogonia. T2-prospermatogonia enter mitosis on day 6 p.p and give rise to A-spermatogonia.
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    URL: http://dx.doi.org/10.1007/BF00219667
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    Development of serotonin-containing cells and the sympathetic innervation of the habenular region in the rat brain (1974)
    Springer
    Cell & tissue research 155 (1974), S. 231-243 
    Details
    ISSN: 1432-0878
    Keywords: Pinealocyte-resembling cells ; Habenular region ; Sympathetic nerve fibres ; Ontogenetic development ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The cells of the pineal gland, the pineal stalk, and the lamina intercalaris contain 5-HT and are innervated by sympathetic nerve fibres. These peripheral nerve fibres continue rostrally from the lamina intercalaris and run into the central nervous tissue of stria medullaris and the habenular nuclei. Pharmacological treatment to increase the cellular 5-HT content revealed that the sympathetic fibres are in close relation to yellow fluorescent cells embedded in the brain tissue. These yellow fluorescent cells develop very late in the ontogenetic development (three weeks or more postnatally) and are preceded by ingrowth of sympathetic fibres into the brain tissue. The results support the hypothesis that the cells found in the habenular region are of pinealocyte rather than neuronal nature, but it is possible that they differ in certain aspects from the cells of the pineal gland proper.
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    URL: http://dx.doi.org/10.1007/BF00221357
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  • 56
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    Electronic Resource
    Somatic spines in the supraoptic nucleus of the rat hypothalamus (1974)
    Springer
    Cell & tissue research 148 (1974), S. 203-211 
    Details
    ISSN: 1432-0878
    Keywords: Hypothalamus ; Rat ; Supraoptic nucleus ; Ultrastructure ; Somatic spines
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Electron microscopic studies of ultrathin serial sections of the perikarya and adjacent neuropil of neurons of the supraoptic nucleus (SON) of the adult male rat revealed varying forms of two types of somatic spines. One type forms synapses with axons passing the cell, the other, without synapses, appears to serve as a buttress or clasp for adjacent neuronal and glial processes. The synapse-bearing spines lack the usual spine apparatus but contain the flocculent substance often seen in spines. The other spines do not exhibit either of these structures. These somatic spines were also seen in Golgi impregnated preparations but the types could not be distinguished. Certain axons synapse either on a somatic spine of the perikarya or penetrate the glial sheath of the neuron and synapse, usually repeatedly, on the soma in an en passant manner.
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    URL: http://dx.doi.org/10.1007/BF00224582
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  • 57
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    Ultrastructure of rat pineal gland after preparation by Freeze-etching technique (1974)
    Springer
    Cell & tissue research 148 (1974), S. 371-379 
    Details
    ISSN: 1432-0878
    Keywords: Pineal gland ; Rat ; Electric coupling areas ; Freeze-etching
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The pineal glands of Wistar rats were fixed in glutaraldehyde and prepared by the freeze-etching technique. With regard to the form and internal structure of pineal cells this technique has revealed nothing essentially new. Synaptic ribbons are very rarely found. A regular diagonal arrangement of membrane attached particles between the Schwann cell and the axon of an autonomic nerve terminal was observed. Three types of contact areas between pineal cells are described. In the first two types, which occur more frequently, the diameter of the area varied from 1000 to 2500 Å, with irregularly aggregated membrane associated particles. The third type, larger but less frequently found, has a surface area of 0.4 μm2 and much more regularly distributed membrane associated particles (ø 88 Å). These contact areas can be considered to be electric coupling areas between pineal cells.
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    URL: http://dx.doi.org/10.1007/BF00224264
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  • 58
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    Electronic Resource
    Indoleamine-containing nerve terminals in the rat median eminence (1974)
    Springer
    Cell & tissue research 147 (1974), S. 285-292 
    Details
    ISSN: 1432-0878
    Keywords: Median eminence ; Rat ; Serotonin terminals ; 5,6- and 5,7-Dihydroxytryptamine ; Chemical degeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Electron microscopical evidence for the existence of an important serotonergic input to the rat median eminence is presented. This evidence is based on the demonstration of degenerating nerve terminals in the external layer of the rat median eminence following the application of 5,6-dihydroxytryptamine or 5,7-dihydroxytryptamine, drugs known to exert more or less selective toxic effects on central serotonin neurons. The finding of a serotonergic innervation of the median eminence in mammals has important implications for 5-hydroxytryptamine as a modulator of synthesis and/or release of hypothalamic, hypophysiotropic hormones.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00582802
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  • 59
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    Electronic Resource
    On the ultrastructure of the rat anococcygeus muscle (1974)
    Springer
    Cell & tissue research 149 (1974), S. 91-104 
    Details
    ISSN: 1432-0878
    Keywords: Anococcygeal muscle ; Smooth muscle ; Rat ; Innervation, adrenergic
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The rat anococcygeus muscle, which is known from previous functional and histochemical investigations to be a smooth muscle with a dense adrenergic innervation, was studied by electron microscopic methods. The muscle was found to be organized in small bundles of 2 to 8 muscle cells, which were interconnected by desmosome-like junctions and by gap junctions. Small Schwann cell-axon complexes with 1 to 5 axons appeared to represent the functional terminal nerve pathway. Such axon complexes were located at the periphery of the small smooth muscle bundles, they never penetrated into them. A ratio of approximately 6 small axon complexes per 100 muscle cells was found. After glutaraldehyde fixation axon varicosities contained mainly small empty vesicles. After treatment with 5-hydroxydopamine and permanganate fixation mainly small vesicles with a dense core were found. The neuromuscular distances ranged from 550 to 5000 Å, with a mean of about 2600 Å. True close neuromuscular contacts at a distance below 500 Å with a fusing of basal laminae were absent. The present observations provide a morphological base for the findings of previous electrophysiological and pharmacological studies on the anococcygeal muscle.
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    URL: http://dx.doi.org/10.1007/BF00209052
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  • 60
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    Ultramorphometric studies regarding the effect of sex-hormones on the adrenal cortex of the rat (1974)
    Springer
    Cell & tissue research 149 (1974), S. 113-120 
    Details
    ISSN: 1432-0878
    Keywords: Adrenal cortex ; Rat ; Sex-hormones ; Ultramorphometry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Ultramorphometrisch wurde nach dem Verfahren von Rohr et al. (1971) die äußere Zona fasciculata von Sprague-Dawley-Ratten bei 5 Gruppen zu je 4 Tieren verglichen: Männchen, Weibchen im Diöstrus, Weibchen im Östrus, männliche und weibliche Kastraten (Kastration am 4. Lebenstag). Die Nebennierenrindenzellen der äu\eren Fasciculata sind bei Weibchen gegenüber denen normaler Männchen hypertroph: Weibchen im Diöstrus zeigen gegenüber Männchen größere Einzelzellen mit vermehrtem glattem endoplasmatischem Reticulum, erhöhten Einzel- und Gesamtvolumina der Mitochondrien sowie einem höheren Lipoidgehalt bei stärkerer Dispersion der Liposomen. Dieselben funktionsspezifischen Strukturen nehmen bei den Weibchen im Östrus noch weiter zu. Die Kastration der Weibchen am 4. Lebenstag führt zu einer Reduktion der funktionsspezifischen morphometrischen Parameter unter die Werte der Diöstrus-Tiere. Männliche Kastraten gleichen die Werte ihrer funktionsspezifischen Strukturen weitgehend denen der Weibchen im Diöstrus an. Nach Kastration am 4. Lebenstag zeigen weibliche Tiere somit etwas niedrigere morphonietrische Parameter in der äußeren Zona fasciculata als die männlichen Kastraten.
    Notes: Summary With the method of Rohr et al. (1971) the outer zona fasciculata of Sprague-Dawley-rats was compared ultramorphometrically in 5 groups of 4 animals each: males, females during diestrus, females during estrus, male and female castrates (castration on 4th day of life). Adrenal cortical cells of the outer fasciculata in females are hypertrophic in comparison with those of normal males: females during diestrus show in comparison with males larger individual cells with increased SER, increased volume of the individual mitochondrium and total volume of mitochondria as well as an increased lipoid content together with a more pronounced dispersion of liposomes. The same function-specific structures show a further increase in females during estrus. Castration of females on the 4th day of life leads to a reduction of the function-specific morphometric parameters below the values of the diestrus animals. Male castrates show adaption of the values of their function-specific structures towards those of females during diestrus. Following castration on the 4th day of life, female animals thus show somewhat lower morphometric parameters in the outer zona fasciculata than male castrates.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00209054
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    The fine structure of adrenal zona glomerulosa in the adult rat (1974)
    Springer
    Cell & tissue research 149 (1974), S. 235-243 
    Details
    ISSN: 1432-0878
    Keywords: Adrenal zona glomerulosa ; Rat ; Normal ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary This paper reports on an electron microscopic study of the adrenal zona glomerulosa in the normal rat, with special emphasis on the structural diversity of mitochondria in its parenchymatous cells. The mitochondria of cristae may be tubulo-vesicular, plate-like and/or straight tubular, any one mitochondrion containing one or more types of cristae. The mitochondrial features of the zona glomerulosa parenchymatous cells are attributed to the biochemical stages of aldosterone synthesis, taking place in different mitochondria of these cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222276
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  • 62
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    Different populations of granules and their distribution in the hypothalamo-neurohypophysial tract of the rat under various experimental conditions (1974)
    Springer
    Cell & tissue research 151 (1974), S. 117-140 
    Details
    ISSN: 1432-0878
    Keywords: Hypothalamo-neurohypophysial system ; Rat ; Granule distribution ; Electron microscopy, morphometry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Die Neurohypophyse und die neurosekretorischen hypothalamischen Kerngebiete von normalen männlichen und weiblichen Ratten, von trächtigen und durstenden Tieren wurden elektronenmikroskopisch und morphometrisch mit der Fragestellung untersucht, ob sich in den Häufigkeitsverteilungen der mittleren Granula-Durchmesser, bezogen auf die Anzahl der Axone, eine zweigipflige Verteilung ergibt. Bei Kontrolltieren zeigen die Perikarya des Nucleus supraopticus zahlreichere und größere Granula als die Zellen des Nucleus paraventricularis, wobei der Unterschied in den Durchmessern der Granula bei den weiblichen Tieren ausgeprägter ist als bei den männlichen. Beide Kontrollgruppen zeigen in der Neurohypophyse ein zweigipfliges Verteilungsmuster der Granula, wobei die beiden Gipfel bei den männlichen Kontrollen dichter beieinander liegen als bei den weiblichen. Bei den trächtigen Tieren weisen beide Kerngebiete Zeichen gesteigerter Aktivität auf und die Granula in den Zellen des Nucleus paraventricularis sind deutlich größer als bei Kontrolltieren. Zu Beginn der Tragzeit werden relativ große, wahrscheinlich vasopressinhaltige Granula in der Neurohypophyse gespeichert, während sich im letzten Drittel der Tragezeit die beiden Populationen dort nicht mehr unterscheiden lassen. Nach 48 Std. Wasserentzug erscheinen beide hypothalamischen Kerne aktiviert. Auffallend ist, daß die Granula im Nucleus supraopticus deutlich kleiner sind als bei Kontrolltieren. Entsprechend fehlen in der Neurohypophyse große Granula, während Axone mit kleinen Granula vermehrt sind. Nach zehntägiger Durstperiode lassen sich im Nucleus supraopticus, neben sehr typischen morphologischen Veränderungen, morphometrisch ebenfalls deutlich kleinere Granula nachweisen. Im Hypophysenhinterlappen dieser Versuchsgruppe sind die granulahaltigen Axone sehr stark vermindert, und in der Häufigkeitsverteilung fehlen die Granula mit großem Durchmesser während der dem Oxytocin zugeordnete Gipfel unverändert erscheint. In Verbindung mit den elektronenmikroskopischen Befunden lassen sich also aus den Verschiebungen der Verteilungsmuster zwischen den sechs Versuchsgruppen die Granula mit kleinerem Durchmesser oxytocinhaltigen Axonen zuordnen und Granula mit größerem Durchmesser zu den Endigungen, die Vasopressin enthalten. Die morphometrischen Befunde in den neurosekretorischen hypothalamischen Kerngebieten bestätigen diese Zuordnung. Die elektronenmikroskopischen und morphometrischen Befunde werden besonders im Hinblick auf die Hormonverschiebungen zwischen dem “reserve pool” und den “easily releasable pool” diskutiert.
    Notes: Summary A morphometric evaluation of electron micrographs has been carried out from neurosecretory terminals in the neurohypophysis and from the supraoptic and paraventricular nuclei in normal male and female rats as well as in pregnant and water deprived rats. The task of this investigation was to find out whether frequency distribution diagrams of the mean diameter of the neurosecretory granules, plotted versus the number of axons, reveal a grossly bimodal distribution. In normal rats the nerve cells of the nucleus supraopticus (S.O.N.) show more numerous and larger granules than the cells of the nucleus paraventricularis (P.V.N), this difference with respect to the diameter being more pronounced in female than in male animals. In the posterior pituitary lobes the neurosecretory granules exhibit a bimodal distribution, the second peak being situated more closely to the first one in male controls. In pregnant animals both nuclei appear to be activated, and the granules of P.V.N. are distinctly larger as compared to those of female control animals. In early pregnancy larger, i.e. presumably vasopressin-containing granules are stored in the neurohypophysis, while in the last phase before delivery oxytocin-containing neurons and vasopressin-containing ones cannot be differentiated any more. After 48 hrs of thirst both hypothalamic nuclei exhibit signs of increased activation, the diameters of the granules in the S.O.N. being distinctly smaller than in normals. In the neurohypophysis granules with large diameters are lacking, while axons with small granules are more frequent. In the S.O.N. of animals, water deprived for 10 days the diameters of the granules are smaller than normal and in the neurohypophysis the number of granule-containing fibres is strongly reduced. In the frequency distribution diagram granules with larger diameters are lacking whereas the peak, attributed to oxytocin, is not essentially altered. However, from the observed alteration on the distribution patterns of the six experimental groups and in connection with the electron microscopic results the granules with smaller diameters can be attributed to oxytocin-containing axons and the larger ones to vasopressin-containing terminals. Analogous morphometric data have been obtained from the neurosecretory nuclei as well. The electron-microscopic and morphometric observations are discussed with regard to hormone-shifts between the reserve pool and the easily-releasable pool.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222039
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    Studies on the juxtaglomerular apparatus (1974)
    Springer
    Cell & tissue research 151 (1974), S. 471-480 
    Details
    ISSN: 1432-0878
    Keywords: Juxtaglomerular apparatus ; Rat ; Influence of deoxycorticosterone (DOC) ; Electron microscopy ; Morphometric analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Die quantitativ-morphologische Analyse des juxtaglomerulären Apparates nach DOC-Behandlung zeigte, daß mit der Hemmung der Reninbildung eine Hypoplasie der epitheloiden Zellen des juxtaglomerulären Apparates einhergeht. Der Granulagehalt der juxtaglomerulären Zellen nahm ab; die Kern-Plasma-Relation änderte sich und die Leiomyofibrillen in den Epitheloidzellen nahmen zu. An den übrigen Anteilen des juxtaglomerulären Apparates, insbesondere der Macula densa, wurden keine morphologischen Veränderungen beobachtet. Die DOC-Behandlung führte darüber hinaus zu einer Bildung von Einschlüssen in den Podocyten, den Mesangiumzellen und den Zellen der Bowman-Membran. Die verschiedenen Möglichkeiten, über welche Faktoren die juxtaglomerulären Zellen nach DOC-Behandlung degranuliert werden, werden diskutiert. Das ultrastrukturelle Bild der Epitheloidzellen nach DOC-Behandlung entspricht deutlich einer gehemmten Sekretion, da das Ergastoplasma und der Golgi-Apparat ganz besonders stark zurückgebildet werden.
    Notes: Summary Quantitative morphological analysis of the Juxtaglomerular apparatus of rats treated with deoxycorticosterone (DOC) and salt revealed hypoplasia of the epithelioid cells as a concomitant of the inhibition of renin synthesis. The granular content of the Juxtaglomerular cells was significantly decreased, while the nucleus-to-cytoplasm ratio and the volume density of leiomyofilaments in the epithelioid cells increased. No morphological alterations were observed in the remaining constituents of the Juxtaglomerular apparatus, including the macula densa. DOC-treatment also induced the appearance of inclusions in the podocytes in the cells of the mesangium, and in the cells of Bowman's capsule. The possible mechanisms of degranulation of the Juxtaglomerular cells after treatment with DOC are discussed. The ultrastructural appearance of the epithelioid cells after treatment with DOC corresponds to an inhibition of secretion, since the rough endoplasmic reticulum and the Golgi apparatus undergo marked regressive changes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222992
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    Einfluß der Splenektomie auf Magensaftsekretion und Ulcusentstehung der Ratte (1974)
    Springer
    Langenbeck's archives of surgery 336 (1974), S. 15-23 
    Details
    ISSN: 1435-2451
    Keywords: Rat ; Splenectomy ; Gastric Ulceration ; Increase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung An Ratten wurde der Einfluß von Splenektomie auf Magensaftsekretion und Ulcusentstehung untersucht. Sowohl nach “restraint-stress” als auch Pylorusligatur wiesen die splenektomierten Tiere eine ausgeprägte Steigerung der Ulcuszahl und -größe gegenüber den Kontrolltieren auf. In der Magensaftanalyse waren dagegen die Werte der splenektomierten Tiere gegenüber den scheinoperierten nur geringgradig gesteigert. — Als mögliche Ursache der vermehrten Ulcusanfälligkeit nach Splenektomie wurde eine Veränderung lokaler, nicht säuresekretorischer Faktoren der Magenwand diskutiert.
    Notes: Summary In Splenectomiced rats incidence of gastric ulceration was compaired with control groups. Restraint stress and ligation of pylorus was used as model. Splenectomiced rats do show an higher incidence in both models. Both frequency and extension of gastric ulcer were accumulated significantly. Analysis of gastric secretion was only unessentially raised in comparisom to control-groups. Change of protective factors of stomach as possible reason is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01291870
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  • 65
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    The influence of blood flow and water net flux on the blood-to-lumen flux of tritiated water in the jejunum of the rat (1972)
    Springer
    Naunyn-Schmiedeberg's archives of pharmacology 274 (1972), S. 357-374 
    Details
    ISSN: 1432-1912
    Keywords: Intestinal Blood Flow ; Blood-to-Lumen Flux ; Tritiated Water ; Solvent Drag ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary 1. Anaesthetized rats were loaded with tritiated water and a jejunal loop was perfused with hypo-, iso-, and hypertonic buffered saline solutions. The blood flow, the disappearance rate (from the blood), and the appearance rate (in the intestinal lumen) were determined simultaneously. 2. A decrease of the blood flow from about 1.7 to 0.2 ml min−1g−1 wet tissue weight diminished both the appearance rate and the disappearance rate of tritiated water. An increase of the blood flow caused the reversed results; however, at high blood flow the appearance and disappearance rate did not reach the value measured in the experiments with decreasing blood flow. 3. A water net flux directed towards the blood retarded and towards the intestinal lumen accelerated the blood-to-lumen flux of tritiated water at low, intermediate, and high blood flow values. 4. The data were analysed by a modified four-compartment-model considering the variation of blood flow and water net flux. The water net flux influences the blood-to-lumen flux of tritiated water by solvent drag during the penetration through the epithelium and by changing the luminal concentration (e.g. dilution) as well as the blood flow rate in the capillaries near the epithelium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00501273
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  • 66
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    Experimental hydrocephalus (1972)
    Springer
    Journal of neurology 202 (1972), S. 21-36 
    Details
    ISSN: 1432-1459
    Keywords: Hydrocephalus, Experimental ; Ethylnitrosourea ; Experimental Hydrocephalus ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung 25 mg/kg Äthylnitrosoharnstoff wurden am 9 1/2. Tag der Schwangerschaft SD-JCL-Ratten injiziert. Die Feten wurden am Termin excidiert und mit dem Stereomikroskop untersucht. 78% der Feten zeigten einen Hydrocephalus. Die histologische Untersuchung in Stufenschnitten des Gehirns zeigte eine Stenose des Aquädukts ohne subependymale Gliose, ödematöse Plexus chorioidei mit prallgefüllten Gefäßen, spärlichem Bindegewebe und geschwollenen Epithelzellen, Verdünnung der telencephalen Wandungen mit schwach entwickelter granulärer Schicht und in der ependymalen Zone verbleibenden Neuroblasten. Die Entwicklung der jungen Ratten wurde studiert. 75% der neugeborenen Ratten zeigten klinisch gewisse Hinweise auf den Hydrocephalus. Stark betroffene Tiere wurden zunehmend somnolent und/oder gelähmt und starben meist vor Ende der 4. Woche. Der Eiweißgehalt des Liquors war nennenswert vermehrt. Es wurden subdurale, subarachnoidale und intraventrikuläre Blutungen beobachtet und als wahrscheinliche Todesursache angesehen. Die histologische Untersuchung der hydrocephalen Jungtiere ergab eine Abflachung der Ependymzellschicht und pralle Füllung corticaler Gefäße von der 2. Woche an. In späteren Stadien wurde eine Dickenabnahme der periventrikulären weißen Substanz, im besonderen im Bereiche des Occipitalhornes nachgewiesen. Im verdünnten Pallium waren Vacuolenbildungen des perineuralen Gewebes zu sehen, Verbreiterung der Virchow-Robinschen Räume und eine dem Status spongiosus ähnliche Degeneration. Verschiedene Formen ependymaler Wucherung wurden gesehen. Die vorliegende Studie stellt die erste postnatale Beobachtung von transplacentar verursachtem kongenitalem Hydrocephalus in der Ratte dar. Die hier beschriebene Technik erwies sich als einfaches und reproduzierbares Mittel, um einen kongenitalen Hydrocephalus in der Ratte zu erzeugen.
    Notes: Summary SD-JCL rats were injected with 25 mg/kg of ethylnitrosourea on day 9 1/2 of gestation. Fetuses were excised near term and examined under dissecting microscope. 78% of viable fetuses were found to be hydrocephalic. On the histologic study of the serial sections of the brain, a stenosis of the Sylvian aqueduct without subependymal gliosis, edematous choroid plexus with engorged vessels, scanty connective tissue and swollen epithelium, thinning of the telencephalic wall with poorly developed granular layers and the neuroblasts remaining in the ependymal zone, were the characteristic findings in the hydrocephalic fetuses. The natural course of the treated young rats was studied. 75% of the newborn rats showed some clinical evidence of hydrocephalus. Severely affected rats became progressively lethargic and/or paralytic, and died usually before the end of 4th week. Protein content of the cerebrospinal fluid was significantly increased. Subdural, subarachnoid and intraventricular hemorrhages were observed and suspected to be the cause of death. On the histological examination of hydrocephalic youngs, flattening of the ependymal cells and engorgement of the cortical vessels were observed as early as in 2-week-old youngs. In more advanced stages, there was noted the thinning of the periventricular white matter, especially around the occipital horn. The thin pallium showed vacuolization of the perineural tissue, enlargement of the Virchow-Robin space and the status spongiosus-like degeneration. Various types of ependymal proliferation was seen. The present study represents the first postnatal observation of the transplacentally induced congenital hydrocephalus in the rat. The present technique proved to be a simple and reproducible model of producing a congenital hydrocephalus in the rat.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00316423
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    Rate of normal longitudinal bone growth in the rat (1972)
    Springer
    Calcified tissue international 10 (1972), S. 238-251 
    Details
    ISSN: 1432-0827
    Keywords: Growth ; Bone ; Rat ; Tetracycline
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Description / Table of Contents: Résumé La vitesse de croissance normale en longueur de la métaphyse proximale du tibia est déterminée chez le rat Sprague-Dawley entre les âges de 20 et 100 jours, en utilisant la méthode à la tétracycline. Le taux de croissance ne varie que légèrement dans les groupes d'âges différents. Il est plus élevé chez les animaux jeunes et décroit considérablement en fonction de l'augmentation de l'âge. Les rats mâles présentent une croissance plus élevée que les femelles. Cette étude a pour but de mettre au point une méthode permettant de déterminer les facteurs expérimentaux, liés à la croissance en longueur du rat.
    Abstract: Zusammenfassung Die normale Längenwachstums-Geschwindigkeit der proximalen Wachstumsplatte der Tibia wurde bei Sprague-Dawley-Ratten in einem Alter zwischen 20 und 100 Tagen mittels der Tetracyclinmethode gemessen. Die Wachstumsgeschwindigkeit variierte nur wenig innerhalb der einzelnen Altersgruppen. Die Geschwindigkeit war bei jungen Tieren am höchsten und nahm mit zunehmendem Alter beträchtlich ab. Männliche Ratten wuchsen schneller als weibliche. Diese Arbeit dient als Grundlage, um die experimentelle Beeinflussung des Längenwachstums der Ratte abschätzen zu können.
    Notes: Abstract The rate of normal growth in length from the proximal growth plate of the tibia in the Sprague-Dawley rat was measured between 20 and 100 days of age using the tetracycline method. The growth rate varied only slightly within different age groups. The rate was highest in young animals and decreased considerably with increasing age. Male rats grew faster than female. This study is intended to provide a base for an evaluation of experimental influence on the growth in length of the rat.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02012553
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    Tierexperimentelle Untersuchungen zur Verbrennungskrankheit. Auswirkung lokaler Kühlung nach Verbrühung (1972)
    Springer
    Research in experimental medicine 159 (1972), S. 114-123 
    Details
    ISSN: 1433-8580
    Keywords: Scalds ; Local cooling ; Rat ; Quantity of leucocytes ; Verbrühung ; Lokale Kühlung ; Ratte ; Verhalten der Leukocyten
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Untersuchungen an Ratten ergaben folgende Befunde: 1. Sofortige lokale Kühlung nach der Verbrühung beeinflußt bei der Ratte im untersuchten Zeitraum von 15 Tagen den Verlauf der Erkrankung gemessen am Parameter der Leukocytenzahl. 2. Die Kühlungszeit muß ausreichend lang sein. 3. Die Kühlung muß früh genug einsetzen, beim Versuchstier Ratte innerhalb 5 min nach Verbrühung. 4. Bei verspätetem Kühlungsbeginn läßt sich auch durch Verlängerung der Kühlungsdauer das Verhalten der Leukocytenzahl im Ablauf des Krankheitsgeschehens nach Verbrühung nicht beeinflussen.
    Notes: Summary Following results are obtained in experiments with rats: Immediate local cooling after scald had a significant influence on progress of “burn-disease”. Time for local cooling must be long enough, onset of local cooling early enough. It is not possible to restore the consequences of a late onset by prolongation of cooling.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01856038
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    Early prenatal ontogeny of central monoamine neurons in the rat: Fluorescence histochemical observations (1972)
    Springer
    Anatomy and embryology 137 (1972), S. 301-316 
    Details
    ISSN: 1432-0568
    Keywords: Monoamines ; Brain ; Ontogeny ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The early ontogeny of the monoamine neuron systems in the rat brain has been analysed using Falck-Hillarp fluorescence histochemistry. Serial sagittal sections of embryos with a crown rump length between 7 and 13 mm, approximately corresponding to gestational days 12 to 15 were obtained from mothers treated with a monoamine oxidase inhibitor given in order to increase the monoamine levels of the embryos. 5-hydroxytryptamine (5-HT)-neurons made their first appearance in the 8 mm embryo, dopamine (DA)-neurons in the 9 mm embryo, and noradrenaline (NA)-neurons in the 11 mm embryo. Small, rounded, weakly fluorescent cell bodies forming sparse aggregations appeared first. Fluorescent processes of two types soon appeared. Short processes from the cell bodies were running perpendicular to the long axis of the brain stem within the cell groups, while long slender axon bundles could be traced ascending through the met- and mesencephalon and into the prosencephalon as well as descending in the myelencephalon andspinal cord. In the 12 mm embryo the primordial DA cell formation of the substantia nigra with its striatal projections, the 5-HT neuron formations of the caudal mesencephalon, met- and myelencephalon as well as the NA neurons of the met- and myelencephalon are relatively well developed. It is concluded that the monoamine-neurons develop mechanisms for synthesis and storage of amines at a very early stage during ontogeny, thus recapitulating the phylogeny of these old systems. Likewise, monoamine oxidase is present early. The presence of neurotransmitters specifying the different developing neurons long before development of their nerve terminal areas and therefore before the establishment of normal synaptic function may indicate a role of these substances during ontogeny other than transmission of nerve impulses.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00519099
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  • 70
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    Die Einwirkung von Kälte auf mit Zinkjodid-Osmium tetroxyd reagierende synaptische Bläschen in den Nervenendigungen im Corpus pineale der Ratte (1972)
    Springer
    Anatomy and embryology 135 (1972), S. 301-306 
    Details
    ISSN: 1432-0568
    Keywords: Rat ; Cold ; Pineal gland ; Synaptic vesicles ; Zinc iodide-osmium tetroxide (ZIO) reaction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung In den synaptischen Bläschen der Nervenendigungen der Epiphyse von Ratten, welche 2 und 4 Tage einer Temperatur von −6° bis −8°C ausgesetzt worden waren, hat man eine signifikante Vermehrung des Zinkjodid-Osmiumtetroxyd (ZIO) reaktiven Materials festgestellt. Der durchschnittliche Durchmesser der ZIO-positiven synaptischen Vesiculae betrug bei den Kontrollratten 234 Å und bei den Ratten, welche 2 und 4 Tage unter der Kälte gelebt hatten, 380 Å, bzw. 379 Å. Die ZIO-Reaktion war bei den Kontrollratten in 49,3% der synaptischen Vesiculae positiv, nach zweitägiger Kälteeinwirkung waren 58,7% und nach viertägiger Kälteeinwirkung 72,1% der synaptischen Vesiculae ZIO-positiv. Auf Grund der vorliegenden Arbeit war es jedoch nicht möglich zu entscheiden, ob die Vermehrung des ZIO-reaktiven Materials mit einer gleichzeitigen Zunahme der biogenen Amine verbunden ist.
    Notes: Summary In the synaptic vesicles of pineal nerve endings of Wistar rats exposed for 2 and 4 days to a temperature of −6° to −8° C, a significant increase of the zinc iodide-osmium tetroxide (ZIO) reactive material was observed. The mean diameter of ZIO reactive synaptic vesicles of control rats was 234 Å; in rats exposed to cold for 2 and 4 days it was 380 Å, respectively 379 Å. In control rats the ZIO reaction was positive in 49,3% of the synaptic vesicles. The reactivity increased to 58,7% in rats exposed to cold for 2 days, and to 72,1% in rats exposed to cold for 4 days. However, the results of the present study do not permit to conclude if an increase of ZIO reactive material is accompanied by a simultaneuos increase of biogenic amines.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00519040
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    Morphometrische Untersuchungen zum histologischen Nachweis des Corticotropin-Releasing Factor im Infundibulum der Ratte (1972)
    Springer
    Anatomy and embryology 137 (1972), S. 1-29 
    Details
    ISSN: 1432-0568
    Keywords: Corticotropin-Releasing Factor ; Median eminence ; Rat ; Histology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung In der Zona externa infundibuli der normalen Ratte kommen geringe Mengen „Gomori-positiver” Granula vor. Nach bilateraler Adrenalektomie erfolgt eine Vermehrung der Granula, die sich durch Gabe von Hydrocortison einschränken oder verhindern läßt. Dieser Befund hat zu der Annahme geführt, daß die Granula das morphologische Äquivalent des Corticotropin-releasing factor (CRF) darstellen. Morphometrische Untersuchungen an 160 weiblichen Wistarratten zeigen, daß die Menge der nach bilateraler Adrenalektomie in der Zona externa infundibuli nachweisbaren „Gomori-positive” Granula von der postoperative Überlebensdauer abhängt. Die Granulamenge nimmt zunächst bis zum 14. Tag p.o. zu und fällt dann wieder ab. Substitution bilateral adrenalektomierter Ratten mit Hydrocortisonacetat-Kristallsuspension bewirkt eine dosisabhängige Verminderung der Granulamenge, wenn die Behandlung am Operationstag begonnen wird. Applikation von Hydrocortisonacetat-Kristallsuspension vom 14.–21. Tag p.o. führt dagegen zu einer gesteigerten Granulavermehrung. Auch bei stärkster Vermehrung sind die Granula stets nur im Infundibulum und nicht in anderen Bereichen des Hypothalamus nachweisbar. Sie besitzen bei bilateral adrenalektomierten Tieren ein charakteristisches Verteilungsmuster mit besonders hohen Konzentrationen in den paramedianen Abschnitten des rostralen Infundibulumdrittels. Aus der Art und Weise ihrer Verteilung ist zu vermuten, daß die Granula 2 Nervenfaserzügen angehören und ein Neurosekret darstellen. Nach den vorliegenden Untersuchungen besteht zwischen dem Verhalten der „Gomori-positiven” Granula in der Zona externa infundibuli und dem aus pharmakologischen Untersuchungen bekannten Verhalten des Corticotropin-releasing factor eine weitgehende Parallelität. Sie legt nahe, die Menge der Granula als Parameter der CRF-Aktivität zu verwenden.
    Notes: Summary In the zona externa infundibuli (outer layer of the median eminence) of the normal rat a minute number of “Gomori-positive” granules can be detected. Following bilateral adrenalectomy their number increases. This augmentation can be restricted or inhibited by administration of hydrocortisone, a finding, which has led to the assumption that the granules are the morphological equivalent of the corticotropin-releasing factor (CRF). Morphometrical studies on 160 female Wistar rats show that following bilateral adrenalectomy the quantity of “Gomori-positive” granules is dependent on the length of the post-operative survival period. Up to the 14th day p.o. the quantity of granules increases; from then on it diminishes. Substitution of bilaterally adrenalectomized rats with hydrocortisone acetate crystal suspension results in a dose-dependent decrease in the quantity of granules if treatment is begun on the day of operation. In contrast, administration of hydrocortisone acetate crystal suspension from the 14th to the 21st day p.o. results in increased augmentation of the granules. Even when maximum augmentation of the granules is reached, they are invariably found only in the infundibulum and not in other regions of the hypothalamus. In animals that have been subjected to bilateral adrenalectomy, the granules show a characteristic pattern of distribution, with particularly high concentrations in the paramedian parts of the rostral third of the infundibulum. The manner in which the granules are distributed suggests that they are a neurosecretory substance localized in two fibre tracts. The present study points to a close parallelism between the behaviour of the “Gomori-positive” granules in the zona externa infundibuli and that of the corticotropin-releasing factor as revealed by pharmacological investigations. It would seem that the quantity of granules can be used as a parameter of CRF activity.
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    URL: http://dx.doi.org/10.1007/BF00523528
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    Neurogenesis in the cerebellum of the rat: An autoradiographic study (1972)
    Springer
    Anatomy and embryology 138 (1972), S. 155-165 
    Details
    ISSN: 1432-0568
    Keywords: Cerebellum ; Neurogenesis ; Autoradiography ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Time of origin of various neuronal elements in the cerebellum of rat was established with the aid of tritiated-thymidine-autoradiography. The earliest nerve cells to form were the Purkinje cells, and they came into existence on days 15 and 16 of gestation. Interstitial nerve cells had their genesis on days 15, 16, 17 and 18, and the marginal cells on day 16 of the embryonic development. The Golgi cells were found to come into existence on days 17, 18 and 19 of gestation. On day 21 of gestation a number of small-medium-sized nerve cells, which were smaller than the Golgi cells but larger than the granule cells, were seen to come into existence. Finally, the earliest stock of granule, basket and stellate cells, primarily in the nodulus, flocculus and para-flocculus, were observed to have been formed on the day 21 of embryogenesis.
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    URL: http://dx.doi.org/10.1007/BF00519378
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  • 73
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    Effect ofl-DOPA on motor behavior in a rat with a unilateral striatal neoplasm (1972)
    Springer
    Acta neuropathologica 22 (1972), S. 269-271 
    Details
    ISSN: 1432-0533
    Keywords: l-DOPA ; Neoplasm, Striatal ; Motor Behavior ; Rat ; Catecholamines
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary After administration ofl-DOPA to a rat with a unilateral striatal neoplasm of the nucleus caudatus-putamen, postural asymmetries and rotating behavior were observed. These observations are discussed in relation to the activity of catecholamines within the central nervous system.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00684530
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  • 74
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    Comparative studies on thein vitro andin vivo morphology of clones of experimental CNS tumours in the rat (1972)
    Springer
    Acta neuropathologica 20 (1972), S. 248-257 
    Details
    ISSN: 1432-0533
    Keywords: Experimental CNS Tumours ; N-Nitrosomethylurea ; Ethylnitrosourea ; Cell Culture ; Clonal Growth ; Aggregation Pattern ; Transplantation ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Two sarcomas, one neurosarcoma and one polymorphous tumour of uncertain classification of the central nervous system of the rat induced by N-nitrosomethylurea or ethylnitrosourea were the source of 14 clones. The cytomorphology and the aggregation pattern of the clonesin vitro are described. The malignancy and histology were checked by homologous transplantation. All the clones formed sarcoma-like structuresin vivo, but it was difficult to decide whether these neoplasias were real sarcomas or very dedifferentiated glial tumours. The differences in cytology observedin vitro were greater than the histological differencesin vivo.
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    URL: http://dx.doi.org/10.1007/BF00686906
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  • 75
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    The influence of blood flow and water net flux on the absorption of tritiated water from the jejunum of the rat (1972)
    Springer
    Naunyn-Schmiedeberg's archives of pharmacology 272 (1972), S. 417-436 
    Details
    ISSN: 1432-1912
    Keywords: Intestinal Blood Flow ; Instestinal Absorption ; Tritiated Water-Solvent Drag ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary 1. Jejunal loops of anaesthetized rats were perfused with hypo-, iso-, and hypertonic buffered solutions containing tritiated water. The blood flow, the disapperance rate (from the intestinal lumen), and the appearance rate (in the intestinal venous blood) were determined simultaneously. 2. A decrease of the blood flow from about 1.7 to 0.2 ml min−1g−1 wet tissue weight diminished the appearance and disappearance rate of tritiated water. An increase of the blood flow caused the reversed results. The appearance rate was almost completely determined by the blood flow indicating that the intestinal absorption of tritiated water is blood flow limited. Therefore the absorption rate of tritiated water characterizes badly the diffusive water permeability of the intestinal epithelium. 3. A water net flux directed towards the blood accelerated and towards the intestinal lumen retarded the intestinal absorption of tritiated water at low, intermediate, and high blood flow values. 4. The data were analysed by a modified four-compartment-model considering the variation of blood flow and water net flux. The water net flux influences the absorption of tritiated water during the penetration through the epithelium by solvent drag (sieving coefficient 1-σ=1.54±0.19) as well as by changing the blood flow rate through capillaries near the epithelium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00501248
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  • 76
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    Effect of forced motility on the noradrenaline and 5-hydroxytryptamine metabolism in different parts of the rat brain (1972)
    Springer
    Psychopharmacology 26 (1972), S. 195-200 
    Details
    ISSN: 1432-2072
    Keywords: Forced Motility ; Motor Activity ; Brain Noradrenaline and 5-Hydroxytryptamine ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The levels of 5-HT and 5-HIAA in the rat brain were measured after 1, 6, 24 and 36 h of forced motility in a treadwheel. The results showed that 5-HT metabolism had already increased in the forebrain after 1 h of exercise but later also increased in the brain stem, especially in the mesencephalon-pons-medulla regions. These changes had almost entirely disappeared after 50 min of rest. The NA levels with and without monoamine oxidase inhibitor were measured in animals after 36 h of forced motility. The results indicated an increase in NA metabolism in the brain stem.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00422106
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  • 77
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    Conflict behavior in a heated-floor maze: Effects of oxazepam (1972)
    Springer
    Psychopharmacology 26 (1972), S. 317-320 
    Details
    ISSN: 1432-2072
    Keywords: Oxazepam ; Conflict Behavior ; Rat ; Maze
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Rats were tested in a Lashley III maze, the floor of which was heated. The animals had to learn to negotiate it in order to gain access to the unheated goal box. In the early period of training the animals were able to find the goal box rapidly and without error. Their performance then deteriorated, in a particular way which strongly evoked a conflict behavior. This deterioration in performance was progressively reduced by oxazepam administration (8 mg/kg/day).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00422708
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  • 78
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    Improvement of performance on an attention task with chronic nicotine treatment in rats (1972)
    Springer
    Psychopharmacology 26 (1972), S. 347-360 
    Details
    ISSN: 1432-2072
    Keywords: Chronic Nicotine ; Attention Task ; Arousal ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Male, Holtzman rats were trained extensively on an attention task which required them to respond with a single lever-press to a very short, variably presented stimulus for food reinforcement, but which also required them to inhibit inappropriate responses. After performance had stabilized, two groups were treated with either nicotine base (100 Μg/kg, s.c., t.i.d.) or saline for 4 weeks. The groups were crossed-over so that the first received saline and the second nicotine for an additional 4 weeks. A 3-week saline recovery period followed. Rats were tested on the attention task daily through all periods. Analysis of the data indicated that independent of the treatment-order, rats performed more efficiently under nicotine treatment than under saline conditions. The improvement in performance was most notable in the reduction of inappropriate responding during chronic nicotine treatment. The significance of the behavioral changes in relation to proposed nicotine-induced neurophysiological changes and the two-arousal hypothesis are discussed.
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    URL: http://dx.doi.org/10.1007/BF00421900
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  • 79
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    Selective increase in avoidance responding by methamphetamine in naive rats (1972)
    Springer
    Psychopharmacology 24 (1972), S. 449-455 
    Details
    ISSN: 1432-2072
    Keywords: Avoidance and Acquisition ; Methamphetamine ; Discrimination Learning ; CNS Stimulants ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Avoidance responses, extra responses (on avoidance lever) and wrong lever responses were measured in naive rats acquiring a discriminated avoidance in a 2 lever Skinner box, 30 min after methamphetamine (0.25, 0.5 and 1.0 mg/kg). The smallest dose was ineffective; 0.5 and 1.0 mg/kg significantly increased the number of avoidance responses. This increase was not associated with a significant increase in the two other responses studied. The progressive increase (from the first to second part of the acquisition session) in avoidance responses was not accompanied by a parallel increase in the other responses. The position discrimination and the discrimination of the presence or absence of the conditioned stimulus was observed to be better in the drug groups.
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    URL: http://dx.doi.org/10.1007/BF00423434
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  • 80
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    Influence de la surrénalectomie, de l'hypophysectomie, de la thyroidectomie, de la castration ainsi que de la testostérone sur le comportement d'agressivité intraspécifique induit par l'apomorphine chez le rat (1972)
    Springer
    Psychopharmacology 24 (1972), S. 476-484 
    Details
    ISSN: 1432-2072
    Keywords: Agressive Behaviour ; Apomorphine ; Testosterone ; Testes ; Hypophysis ; Adrenal Glands ; Thyroid ; Castration ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The weights of testes, prostates, adrenals, thymus and the pituitary of rats with apomorphine induced aggressiveness did not differ from those without aggressiveness. Postpubertal castration, adrenalectomy or thyroidectomy did not reduce the apomorphine induced aggressive behaviour, but a considerable reduction was found after hypophysectomy. Prepubertal castration significantly reduced, but did not completely abolish the proportion of aggressive rats, especially when castration was performed on the 5th day. Prolonged treatment of male, female and castrated rats with high doses of testosterone from the time of weaning increased the proportion of aggressive animals. Less intensive treatment and treatment of adult rats were ineffective.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00423438
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  • 81
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    Shock-elicited fighting and delta-9-tetrahydrocannabinol (1972)
    Springer
    Psychopharmacology 25 (1972), S. 218-228 
    Details
    ISSN: 1432-2072
    Keywords: Fighting ; Delta-9-Tetrahydrocannabinol ; Chlordiazepoxide ; Fixed-Interval ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The frequency with which electric shock to the feet elicited fighting in five pairs of albino rats was not altered significantly by intraperitoneal injections of delta-9-tetrahydrocannabinol (THC) in doses ranging from 0.064–6.4 mg/kg, although chlordiazepoxide reduced the frequency of such fighting in a dose-related manner. This finding held true despite manipulations of THC vehicle, injection-test interval, and the previous drug experience of the subjects. In contrast, doses of 4.0 mg/kg produced a striking reduction in lever-pressing maintained by an FI 60″ schedule of food reinforcement.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00422503
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  • 82
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    Influence of disodium ethane-1-hydroxy-1,1-diphosphonate on vitamin D metabolism in rats (1972)
    Springer
    Pflügers Archiv 336 (1972), S. 249-262 
    Details
    ISSN: 1432-2013
    Keywords: Diphosphate ; Rat ; Vitamin D Metabolism ; Intestinal Calcium ; Absorption ; Bone Mineralization ; Renal Functions
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The metabolism and the organ distribution of double labelled vitamin D3 (1,2-3H-4-14C-cholecalciferol) has been studied in rats in which the bone mineralization and the intestinal calcium absorption have been inhibited by a large pose (10 mg P/kg s.c. for 7–14 days) of disodium ethane-1-hydroxy-1,1-diphosphonate (EHDP). The most striking difference found was a reduced accumulation of radioactive cholecalciferol and its metabolites in the kidney of EHDP-treated rats. It is unlikely that this effect was due to an unspecific alteration of the functional renal tissue since blood urea, glomerular filtration rate and renal plasm a flow remained unaltered by this dose of EHDP. The EHDP-treated rats were able to form the metabolite eluted with peak IV of the silicic acid chromatographic system, that is 25-hydroxycholecalciferol. In these vitamin D repleted rats fed a high calcium diet, the tritium deficient metabolite eluted with peak V (1,25-dihydroxycholecalciferol) was only found in the intestinal mucosa of both control and EHDP groups three days after the injection of radioactive cholecalciferol, and this in a very small amount. Therefore no definitive conclusion can be drawn as to a possible interference of EHDP treatment on the production of 1,25-dihydroxycholecalciferol. The change in the renal metabolism of vitamin D in rats treated with a rachitogenic dose of EHDP may be caused by the modifications of the calcium metabolism brought about by the diphosphonate. Its relation, if any, with the decreased calcium absorption remains to be established.
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    URL: http://dx.doi.org/10.1007/BF00590049
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    Cardiac output, arterial and mixed-venous O2 saturation, and blood O2 dissociation curve in growing rats adapted to a simulated altitude of 3500 m (1972)
    Springer
    Pflügers Archiv 335 (1972), S. 10-18 
    Details
    ISSN: 1432-2013
    Keywords: Cardiac Output ; Blood O2 Dissociation Curve ; High Altitude ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In rats adapted to a simulated altitude of 3500 m cardiac output measured at hypoxia by the direct Fick principle was significantly lower than in the control animals (mean values 54.3 ml/min and 69.8 ml/min, resp.). The decrease of cardiac output was accompanied by an increase of arterio-venous O2 difference and a decrease of stroke volume in the adapted rats. It is suggested that the decrease of cardiac output might be related to the increase of hematocrit. The adapted rats also showed higher arterial and mixed-venous O2 content (both at hypoxia) and increased O2 capacity. Arterial O2 saturation of the animals previously exposed to simulated high altitude hypoxia was significantly higher (67.3% as against 61.2% in the controls). The standard O2 dissociation curve showed lower oxygen affinity in the blood of the adapted animals but no physiological advantage concerning the transport of O2 to the tissues was found. In another group of animals the Bohr factor was estimated and no difference was found between rat and human blood.
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    URL: http://dx.doi.org/10.1007/BF00586931
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  • 84
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    Untersuchungen zur Transport-Kinetik mittelkettiger Fettsäuren am Dünndarm (1972)
    Springer
    Pflügers Archiv 335 (1972), S. 198-212 
    Details
    ISSN: 1432-2013
    Keywords: Intestinal Absorption ; Medium Chain Fatty Acids ; Kinetics ; Active Transport ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary 1. The transport of14C-octanoate by rat small intestine was determined in vitro by a modification of the method of Semenza and Mühlhaupt, and in vivo by using isolated segments or a perfusion technique in conscious rats (Haberichet al., 1968). 2. The absorption capacity of14C-octanoate in the distal ileum was significantly lower than in the proximal jejunum. 3. 14C-octanoate injected into a jejunal segment appeared rapidly in portal venous blood reaching its maximal concentration 4 min after the injection. 4. The transport of octanoate showed a saturation effect with increasing concentrations after an incubation of 4 min. However, with constant concentrations of octanoate in the medium, the transport rate was constant for 8–10 min. A similar saturation effect was also demonstrated in vivo. Values for maximal absorption rate (V max) and the half-saturation constant (K m), calculated from the Lineweaver-Burk plot, were 1.0 μMol/min and 17.7 mM, respectively. 5. Accumulation of octanoate in tissue water was demonstrated. For example, after incubation for 40 min in a medium concentration of 0.1 mM, the tissue/ medium ratio was 2.75:1. 6. Cyanide (2×10−4 M) markedly diminished the absorption rate of octanoate. 7. The absorption of octanoate was competitively inhibited by caproic acid (C6) in vitro. The inhibition constant (K i), calculated from the Dixon plot, was 47 mM. 8. Simultaneous administration of glucose and octanoate in vivo did not affect the absorption rate of the fatty acid. 9. These results suggest that the intestinal absorption of octanoate is an energy-dependant active process.
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    URL: http://dx.doi.org/10.1007/BF00592157
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    Hypoxic pulmonary steady-state diffusing capacity for CO and alveolar-arterial O2 pressure differences in growing rats after adaptation to a simulated altitude of 3500 m (1972)
    Springer
    Pflügers Archiv 335 (1972), S. 1-9 
    Details
    ISSN: 1432-2013
    Keywords: Pulmonary Diffusing Capacity ; Alveolar-arterial O2 Pressure Difference ; High Altitude ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Steady-state pulmonary diffusing capacity for CO and alveolar-arterial O2 pressure differences were measured at hypoxia in growing rats adapted to a simulated altitude of 3500 m. The pulmonary diffusing capacity was significantly higher and the alveolar-arterial gradients were significantly lower in the adapted animals as compared with the controls exposed to hypoxia for the first time. The increased diffusing capacity could be explained entirely by the increase of blood O2 and CO capacity whereas the decrease of gradients might be explained by the increase of blood O2 capacity together with an increase of the arterio-venous O2 difference.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00586930
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  • 86
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    Cardiac hypertrophy, capillary and muscle fiber density, muscle fiber diameter, capillary radius and diffusion distance in the myocardium of growing rats adapted to a simulated altitude of 3500 m (1972)
    Springer
    Pflügers Archiv 335 (1972), S. 19-28 
    Details
    ISSN: 1432-2013
    Keywords: Cardiac Hypertrophy ; Diffusion Distance ; High Altitude ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In growing rats adapted to a simulated altitude of 3500 m for about 4 weeks and in their controls the evolution of cardiac ventricular weight was followed. The increase of total ventricular weight found in the adapted animals can be attributed exclusively to the increase of right ventricular weight. In other adapted and control animals cardiac capillary densities, muscle fiber diameter and external capillary radius were estimated and fiber—capillary ratio and diffusion distance were calculated. There was an increase of capillary density together with a decrease of muscle fiber density, fiber-capillary ratio and diffusion distance in the right but not in the left ventricle of the adapted rats. The muscle fiber diameters, however, were larger in both heart ventricles of the rats exposed to a simulated high altitude, especially in the right ventricle. This indicates that true hypertrophy of the muscle fibers is mainly responsible for the increase of right ventricular weight. In the left ventricle, however, a hypertrophy of the muscle fibers together with a decrease of stroma components is demonstrated. The physiological importance of the shorter diffusion distance in the right ventricle of the high altitude adapted rats is discussed and it is suggested that the shorter diffusion distance may help to keep the tissue O2 partial pressure above the critical value, mainly also in extreme situations with high myocardial O2 consumption.
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    URL: http://dx.doi.org/10.1007/BF00586932
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  • 87
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    Stability of firing patterns of hypothalamic neurons subjected to direct electrical or chemical stimulation (1972)
    Springer
    Experimental brain research 15 (1972), S. 29-38 
    Details
    ISSN: 1432-1106
    Keywords: Ventromedial hypothalamus ; Single units ; Spontaneous and stimulated activity ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The relationship between mean firing rate and activity pattern was studied in 177 neurons in the ventromedial hypothalamus of rats immobilized by Remyolan. Stationary epochs of spontaneous or stimulated activity containing at least 512 interspike intervals were analyzed with the LINC computer. The most frequently encountered interval histograms (IHs) were of the exponential (35%) or gamma 2 (25%) type. The IH shape remained unchanged even if the average firing rate was varied over a wide range (from 20/sec to 2/sec) by polarization through the recording microelectrode (2–5 nA, 1–10 min) or by intracarotid injection of glucose (0.1 ml, 10–25%). The coefficient of variation (CV = SD/ mean interval) was 1.03 for the spontaneous and 0.90 or 0.95 for the activity influenced by polarization or glucose, respectively. Since according to other studies sensory stimulation decreases CV by reorganization of the synaptic input, it is suggested that polarization and glucose affect predominantly the postsynaptic elements without significantly changing the presynaptic activity pattern.
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    URL: http://dx.doi.org/10.1007/BF00234956
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  • 88
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    Der Einfluß von kolloidalem Ferrihexacyanoferrat (II) auf die Verteilung und Toxicität von Thallium (1972)
    Springer
    Archives of toxicology 28 (1972), S. 39-45 
    Details
    ISSN: 1432-0738
    Keywords: Ferrihexacyanoferrate (II) ; Rat ; Thallium ; Toxicity ; Metabolism ; Ferrihexacyanoferrat(II) ; Ratte ; Thllium ; Toxicität ; Stoffwechsel
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Die Verabfolgung einer 2% igen kolloidalen Lösung von Ferrihexacyanoferrat (II) (FHCF) als Trinkwasser erhöht bei der Ratte die Ausscheidungsrate von intravenös injiziertem Tl2SO4 (mit 204Tl markiert) aus Leber, Nieren und Muskulatur auf das 2,8fache. Dieser Effekt ist zeitunabhängig. Die LD 50/30 d von intravenös injiziertem Tl2SO4 wird durch FHCF-Behandlung um das 2,3fache erhöht. Für diesen Schutzeffekt ist allerdings nur das während der ersten 24 Std verabfolgte FHCF verantwortlich. Diese Befunde weisen darauf hin, daß die Schäden bei der akuten Tl-Intoxikation sehr schnell irreversibel werden.
    Notes: Abstract The administration of a 2% colloidal solution of ferrihexacyano ferrate (II) (FHCF) as drinking water increases in the rat the excretion of intravenously injected Tl2SO4 (labelled by 204Tl) from liver, kidneys and muscles by a factor of 2.8. This effect is independant of time. FHCF-treatment increases the LD 50/30 d of intravenously injected Tl2SO4 by a factor of 2.3. This protection, however, is provided by FHCF only if administered during the first 24 hours. These findings suggest that the damage resulting from acute Tl-intoxication quickly becomes irreversible.
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    URL: http://dx.doi.org/10.1007/BF00349626
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    Teratological studies with dichlorvos vapour in rabbits and rats (1972)
    Springer
    Archives of toxicology 30 (1972), S. 29-38 
    Details
    ISSN: 1432-0738
    Keywords: Dichlorvos ; Teratology ; Inhalation ; Rat ; Rabbit ; Dichlorvos ; Teratologie ; Inhalation ; Ratte ; Kaninchen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung An Kaninchen und Ratten wurde die teratogene Wirkung von inhaliertem Dichlorvos untersucht. Im Versuch waren primipare Kaninchenweibchen der Rasse “Dutch” mit einem Anfangsgewicht von 2 bis 3 kg und primipare Rattenweibchen vom Stamm “Carworth Farm E“ mit einem Anfangsgewicht von 200 bis 300 g. Die Tiere wurden während der gesamten Trächtigkeit folgenden Konzentrationen in der Atemluft ausgesetzt: 0,25, 1,25 und 6,25 μg Dichlorvos pro Liter Luft. Ein zusätzliches Experiment am Kaninchen wurde mit 2 und 4 μg Dichlorvos pro Liter Luft durchgeführt. Die Behandlung wirkte weder beim Kaninchen noch bei der Ratte teratogen. Selbst Konzentrationen, die für die Kaninchen tödlich waren, und die eine deutliche Cholinesterasehemmung in Plasma, Erythrocyten und Gehirn der Ratten und Kaninchen hervorriefen, wurden ohne Schädigung für die Feten vertragen.
    Notes: Abstract The teratogenic potential of inhaled dichlorvos vapour for rabbits and rats was investigated on the offspring of primiparous female Dutch rabbits weighing 2 to 3 kg and primiparous female Carworth E strain rats weighing 200 to 300 g. The animals were exposed throughout pregnancy at concentrations of 0.25, 1.25 and 6.25 μg dichlorvos per litre of air. In an additional experiment pregnant rabbits were exposed to concentrations of 2 and 4 μg dichlorvos per litre of air. The results gave no indication that dichlorvos vapour is teratogenic in rabbits or rats even at exposure concentrations resulting in maternal deaths in rabbits, and causing depression of plasma, erythrocyte and brain cholinesterase activities in pregnant animals of both species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00605271
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  • 90
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    Electronic Resource
    The acute toxicity of drugs acting at cholinoceptive sites and twenty-four hour rhythms in brain acetylcholine (1972)
    Springer
    Archives of toxicology 29 (1972), S. 39-49 
    Details
    ISSN: 1432-0738
    Keywords: Mouse ; Rat ; Brain ; Light-Dark-Programming ; Circadian ; Toxicity Rhythms ; Acetylcholine ; Pilocarpine ; Oxotremorine ; Carbachol ; Physostigmine ; Neostigmine ; Atropine ; Scopolamine ; Atropine Methyl Nitrate ; Maus ; Ratten ; Gehirn ; Beleuchtungsprogramm ; Circadianer Toxicitätsrhythmus ; Acetylcholin ; Piloearpin ; Oxotremorin ; Carbachol ; Physostigmin ; Neostigmin ; Atropin ; Scopolamin ; Atropin-methylnitrat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Nach Adaptation an ein Licht-Dunkelprogramm (8.00 bis 20.00 Licht, 20.00 bis 8.00 Dunkelheit) wurde der Acetylcholingehalt (ACh) im Mittelhirn und N. caudatus von Ratten sowie im Gesamthirn von Mäusen bestimmt. Die höchsten ACh-Konzentrationen wurden bei Ratten um 24.00 gefunden; die höchsten ACh-Werte bei der Maus waren nicht signifikant von den Konzentrationen um 24.00 und 12.00 verschieden. Signifikante ACh-Minima traten im Mittelhirn der Ratte um 12.00, im N. caudatus sowie im Mäusegehirn um 18.00 Uhr auf. Während der Dunkelperiode wurde die größte Toxicität gegenüber i.v. bzw. i.p. injiziertem ACh bei Mäusen gefunden. Ähnliche zeitliche Unterschiede ergaben sich für Neostigmin, Pilocarpin und Oxytremorin. Die Toxicität von Carbachol zeigte Maxima bei 12.00 und 24.00. Für Physostigmin, einen CholinesteraseHemmstoff mit einem tertiären Stickstoff, lagen sowohl die Toxicitätsmaxima als auch die Toxicitätsminima in der Dunkelperiode. Die rhythmische Schwankung der Toxicität von i.p. verabfolgtem Atropin verhielt sich umgekehrt wie die der Cholinomimetica (außer Carbachol). Auch nach i.v. Applikation von Scopolamin und N-Methylatropin war ein entsprechender rhythmischer Einfluß auf die Toxicität nachweisbar. Die Vorbehandlung mit N-Methylatropin schwächte die Toxicität zwar ab, der Toxicitätsrhythmus gegenüber ACh und Physostigmin war aber nicht verändert. Die Toxicität von Cholinomimetica zeigt ein Maximum während der höchsten ACh-Konzentrationen im Gehirn und der höchsten Stoffwechselaktivität. Bei den anticholinergen Substanzen zeigte sich ein umgekehrtes Verhältnis. Die Beziehungen zum zentralen ACh-Gehalt sind unklar.
    Notes: Abstract Acetylcholine (ACh) levels were determined in rat midbrain and caudate nucleus and mouse whole brain in mature animals adapted 3 weeks to a programmed lighting schedule (08:00 to 20:00 h light; 20:00 to 08:00 dark). Peak ACh levels in the rat occurred at 24:00 h; in the mouse the peak at 06:00 h did not differ significantly from values obtained at 24:00 and 12:00 h. Significant trough values occurred at 12:00 h in rat midbrain and at 18:00h in rat caudate nucleus and mouse brain. In mice toxicity of I.V. and I.P. administered ACh is maximal during the dark. Neostigmine, pilocarpine and oxotremorine have toxicity patterns similar to ACh. Carbachol toxicity peaked at 12:00 and 24:00 h. With physostigmine, a tertiary anticholinesterase, peak and trough occurred during the dark. The atropine (I.P.) toxicity rhythm is a mirror image of the cholinomimetic rhythm (except for carbachol). I.V. scopolamine and atropine methyl nitrate patterns resemble atropine's but are less clear-cut. Atropine methyl nitrate pretreatment reduced ACh and physostigmine toxicity but did not alter the overall pattern. Cholinomimetic toxicity and brain ACh patterns are similar, peaking when metabolic activity is maximal. An inverse relationship holds for the anticholinergic drugs. The relationship to central ACh is uncertain.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00316513
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  • 91
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    Electronic Resource
    The comparative metabolism of [14C-vinyl]dichlorvos in animals and man (1972)
    Springer
    Archives of toxicology 30 (1972), S. 9-18 
    Details
    ISSN: 1432-0738
    Keywords: 2,2-Dichlorvinyl Dimethyl Phosphate ; Dichlorvos ; Vapona ; Metabolism ; Man ; Rat ; Mouse ; Hamster ; 2,2-Dichlorvinyl-dimethylphosphat ; Dichlorvos ; Vapona® ; Stoffwechsel ; Mensch ; Ratte ; Maus ; Hamster
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung An Mäusen und Hamstern wurde die Exkretion und Retention der Radioaktivität nach Fütterung mit [1-14C-Vinyl]-Dichlorvos bestimmt. Außerdem wurde beim Menschen die Exkretion und Exhalation gemessen und die erhaltenen Werte mit den tierexperimentellen Daten von Rattenversuchen verglichen. Die Ergebnisse zeigen einen schnellen Stoffwechsel von Dichlorvos bei allen vier untersuchten Species. Die Dealkylierung zu Desmethyldichlorvos spielt bei der Biotransformation eine untergeordnete Rolle. Eine gewisse Ausnahme zeigen die Mäuse, bei denen 18,5 % der zugeführten Radioaktivität als Desmethyldichlorvos aufgefunden werden konnte. Bei allen Species kam es zu einer hydrolytischen Spaltung zu Dichloracetaldehyd, der weiter zu Dichloraetbanol reduziert wird. Der weitere Weg führt über die Ausscheidung als Glucuronid mit dem Harn. Ein Teil wird dechloriert und das14C-Atom als14CO2 oder14C-Hippursäure im Harn ausgeschieden. Im menschlichen Harn konnte Dichloraethanol nach Inkubation mitβ-Glucuronidase nachgewiesen werden, wenn hohe Dichlorvoskonzentrationen (38 μg/l) eingeatmet wurden. Auf dieser Basis könnte eine Überwachungsmethode gegen die Inhalation von zu hohen Dichlorvoskonzentrationen ausgearbeitet werden.
    Notes: Abstract The routes of excretion and retention of radioactivity following the ingestion of [1-14C-vinyl]dichlorvos by mice and hamsters have been determined. The excretion of radioactivity in urine and exhaled air of man has also been measured. The data have been compared with those for rats. The results indicate that metabolism is rapid in the four species. Dealkylation to desmethyldichlorvos is a minor route of biotransformation, except in the mouse, where desmethyldichlorvos constitutes at least 18.5 % of the administered radioactivity. Hydrolytic metabolism of dichlorvos occurs in all species and leads presumably to dichloroacetaldehyde which is further metabolized by (i) reduction to dichloroethanol and excretion in the urine as the glucuronide and (ii) dechlorination followed by incorporation of the carbon atoms into endogenous biosyntheses and excretion as [14C]carbon dioxide and [14C]hippuric acid in the urine. Dichloroethanol can be detected inβ-glucuronidase-treated urine after exposure of a human subject to high air concentrations (38 μg/l) of non-radioactive dichlorvos. This may form the basis of a method of monitoring the exposure of humans to high concentrations of dichlorvos.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00605269
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  • 92
    Electronic Resource
    Electronic Resource
    Effects of aging on dynamic viscoelastic properties of the rat's thoracic aorta (1972)
    Springer
    Pflügers Archiv 331 (1972), S. 357-364 
    Details
    ISSN: 1432-2013
    Keywords: Thoracic Aorta ; Complex Dynamic Young's Modulus ; Age ; Serum Lipids ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In 2 groups of male albino rats (wistar-strain), 6 months and 18 months of age, dynamic Young's moduli in longitudinal direction were measured, at 5 different frequencies between 1 and 5 Hz. Each group consisted of 10 animals. Measurements were made at the natural length and at 1.2 times this length. From each animal several serum lipids were determined. It was found that: 1. measured at the natural length and 1.2 times this length, no significant difference of dynamic Young's moduli between both age groups could be demonstrated 2. concerning the dynamic Young's moduli there is significant interaction between stretch level and age 3. retraction was significantly smaller in the old rats than in the young ones 4. the old animals had a significantly higher serum lipid content than the young rats 5. no correlation between serum lipids and Young's moduli could be demonstrated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00592696
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  • 93
    Electronic Resource
    Electronic Resource
    Effects of electrochemical stimulation of the hypothalamus on serum prolactin and LH in rats (1972)
    Springer
    Pflügers Archiv 337 (1972), S. 71-79 
    Details
    ISSN: 1432-2013
    Keywords: Rat ; Hypothalamus ; Prolactin ; LH
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Plasma levels of prolactin and LH were measured by radioimmunoassay following electrochemical stimulation of the medial preoptic area (MPO) or the arcuate nucleus (Arc.N.) in pentobarbital anesthetized proestrous rats. Differences in the secretion pattern of prolactin and LH were observed when stimulated by means of acutely or chronically implanted electrodes. Acute implantation and stimulation of the MPO resulted in no change in serum prolactin levels, whereas stimulation by means of chronically implanted electrodes evoked a marked increase in serum prolactin. The general observation was that electrostimulation in the acute experiments causes a less sharp but more prolonged prolactin and LH release from pituitary than stimulation through chronically implanted electrodes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00587874
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  • 94
    Electronic Resource
    Electronic Resource
    Mesure de la vitesse de renouvellement du lactate chez le rat par perfusion de 14-C-U (l) lactate (1972)
    Springer
    Pflügers Archiv 334 (1972), S. 293-302 
    Details
    ISSN: 1432-2013
    Keywords: Lactate Turnover ; l-Lactate14C (U) ; Hypoxaemia ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary 1. Lactate turnover was measured in 24 anesthetized rats using14C-U-(l) Lactate with the priming dose-infusion technique. 2. 14C Lactate in blood was determined with a specific radio-enzymatic micromethod. 3. Lactate turnover increases with blood lactate concentration in the range 0.5 to 3 mM. In a steady state system this relationship means that a new equilibrium may be achieved at a higher pool level, between lactate production and utilization. 4. Comparing available data in four mammalian species lactate turnover at normal lactate levels was found to be approximately constant when computed on a body surface unit basis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00592164
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  • 95
    Electronic Resource
    Electronic Resource
    Sexual behaviour of adult rats after a single neonatal injection of reserpine (1972)
    Springer
    Psychopharmacology 23 (1972), S. 171-179 
    Details
    ISSN: 1432-2072
    Keywords: Reserpine ; Neonatal Treatment ; Sexual Behaviour ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract To investigate whether reserpine given in the critical period of sexual differentiation of the rat brain affects adult sexual behaviour, female and male rats were given a single injection of reserpine on the fourth day after birth, and their sexual behaviour was tested at maturity. In the 11 consecutive daily tests for spontaneous sexual behaviour the reserpine treated female animals showed fewer regular behavioural and vaginal oestrous cycles than the control animals. 19 % of the reserpinized and none of the control animals had prolonged oestrous behaviour without a coincident prolonged vaginal cycle. The reseptivity quotients of the reserpine treated animals were reduced. In the tests for hormone induced sexual behaviour after ovariectomy no statistically significant differences between the number of animals responding or between the receptivity quotients occurred. The tests for spontaneous sexual behaviour in males showed that the reserpinized animals needed fewer intromissions to ejaculate than the controls. The significance of these findings is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00401192
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  • 96
    Electronic Resource
    Electronic Resource
    Distribution and elimination of trimethoprim in pregnant and newborn rats (1972)
    Springer
    Naunyn-Schmiedeberg's archives of pharmacology 272 (1972), S. 369-377 
    Details
    ISSN: 1432-1912
    Keywords: Trimethoprim ; Placental Transfer ; Distribution ; Elimination ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary After a single i.v. administration of 50 mg/kg of Trimethoprim (TM) to rats in late pregnancy, a diffusion equilibrium of the folic acid inhibitor in the foetal organs is reached after 30–60 min. The TM levels in maternal as well as in foetal organs can have an inhibitory effect on folic acid reductase activity. The elimination of TM is lower in pregnant than in non-pregnant animals. The TM elimination of the foeti is dependent on the elimination capacity of the mother. Three-days-old rats eliminate TM some four times slower than mature animals owing to their reduced kidney development.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00501243
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  • 97
    Electronic Resource
    Electronic Resource
    Calcium transport in rat small intestine in vitro and in vivo (1972)
    Springer
    Naunyn-Schmiedeberg's archives of pharmacology 273 (1972), S. 352-365 
    Details
    ISSN: 1432-1912
    Keywords: Calcium Transport ; Small Intestine ; Rat ; in vitro ; in vivo
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Intestinal calcium (Ca) transport was studied in the rat jejunum by the in vitro perfusion technique of Fisher and Parsons and in the tied loop in vivo. Mucosal uptake and absorption of Ca was examined under the following conditions: rising intraluminal Ca-concentrations (0.5–128 meq/l); inhibition of energy dependent metabolism (2,4-dinitrophenol, N2, low temperature); net water flow, out of or into the intestinal lumen; addition of strontium (Sr); pretreatment with low Ca-diet and with 6-methyl-prednisolone. The concentration-dependent Ca absorption curve rose steeply at low Ca-concentrations but changed to a slowly rising straight line above 16 meq/l Ca++. In contrast, Ca uptake into the intestinal wall was directly related to Ca concentration, was linear from the beginning and paralleled the straight part of the absorption curve. Ca absorption was decreased by inhibition of energy dependent metabolism, addition of Sr and pretreatment with prednisolone. Pretreatment with low Ca diet increased Ca absorption and direction of net water flow (“solvent drag”) had no effect on it. Mucosal uptake of Ca was similar to Ca absorption except that metabolic inhibition increased Ca uptake but decreased Ca absorption. These results are compatible with the concept of a passive mucosal uptake and of an active absorption of Ca at low intraluminal Ca concentrations with additional passive component at high Ca concentrations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00499669
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  • 98
    Electronic Resource
    Electronic Resource
    Concentration and origin of choline in the rat brain (1972)
    Springer
    Naunyn-Schmiedeberg's archives of pharmacology 274 (1972), S. 91-106 
    Details
    ISSN: 1432-1912
    Keywords: Choline ; Blood-Brain-Barrier ; Brain ; Rat ; Lecithin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In the rat brain the concentration of choline increases with a velocity of 20.5 nMol/g×min immediately after decapitation. This is not due to postmortal disintegration of the tissue but to persisting biochemical reactions in the absence of blood flow. This rise of choline must be considered if the concentration of choline in the living animal is to be established. Taking this fact into account one arrives at a value of 27.5±1.7 nMol Choline/g wet weight. From the arterio-venous difference of choline concentrations it has been postulated that there is an efflux of 7.2±1.5 nMol/g×min from the brain into the blood. After i.v. injection of labelled choline the peak of the specific radioactivity of choline in the brain is reached in less than 1 min but amounts to only one tenth of the specific activity of choline in blood-plasma. The specific activities in both compartments decline in parallel. Thus it can be concluded a) that choline penetrates easily through the blood-brain barrier, but the concentration gradient is directed from brain to blood; and b) that choline is continuously formed in the brain, which process permanently dilutes the quantity of labelled choline and prevents the specific activities in blood and brain from becoming equal. The calculations of turnover-rates in the different reactions leading to the choline containing glycerophosphatides are based on the data obtained. On the basis of these findings it is proposed that in the brain phosphorylated derivatives of ethanolamine (phosphorylethanolamine, cytidinediphosphate-ethanolamine) can be methylated to form the respective choline derivatives.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00501010
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  • 99
    Electronic Resource
    Electronic Resource
    Immersion and perfusion fixed rat adrenal medulla: The problem of mixed cells, clear cells, and the mode of secretion (1972)
    Springer
    Cell & tissue research 126 (1972), S. 261-277 
    Details
    ISSN: 1432-0878
    Keywords: Adrenal medulla ; Rat ; Cell types ; Secretion ; Influence of fixation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Das Nebennierenmark der Ratte wurde nach Anwendung verschiedener Fixationsmethoden untersucht. Nach Immersionsfixation mit Glutaraldehyd oder Osmiumtetroxyd finden sich in der Nebenniere sog. Mischzellen, helle Zellen, syncytiale oder plasmodiale Zellen, die von den Untersuchern als Artefaktbildungen angesehen werden. In allen Proben nach Perfusionsfixation wurden solche Artefaktbildungen niemals beobachtet. Bei Immersionsfixation wurde eine enge Beziehung zwischen Sekretgranula und Cytoplasmamembran beobachtet, was bei Perfusionsfixation selten der Fall war. Die heutigen Theorien über den Sekretionsmechanismus bei Nebennierenmarkzellen werden aufgrund der vorgelegten Ergebnisse diskutiert. Die Arbeit zeigt die wesentlichen Vorteile der Anwendung der Perfusionsfixation für die Untersuchung der Nebenniere.
    Notes: Summary The adrenal medulla of the rat was studied utilizing various methods of fixation. In adrenal medulla specimens after immersion fixation either with glutaraldehyde or osmium tetroxide, elements such as mixed, clear, syncytial, or plasmodial cells, believed to be of artifactual origin, are observed in all of this material examined. These elements are absent in the specimens prepared by perfusion fixation. In specimens prepared by immersion fixation, secretory granules are found in close proximity to the plasma membrane; this localization is infrequent after perfusion fixation. Current theories of the mechanism of secretion of adrenal medullary hormones are discussed on the basis of our results. This investigation demonstrates the advantage and necessity of perfusion fixation in the study of the adrenal medulla.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307220
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  • 100
    Electronic Resource
    Electronic Resource
    The fine structure of macrophages in the enamel organ, with special reference to the microtubular system (1972)
    Springer
    Cell & tissue research 126 (1972), S. 466-482 
    Details
    ISSN: 1432-0878
    Keywords: Macrophages ; Microtubules ; Enamel organ ; Rat ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In the enamel organ of rat incisors macrophages are present in the zone of matrix formation, the transitional zone, the enamel maturation and pigmentation zone. The macrophages accumulate adjacent to redifferentiating amelocytes in the transitional zone. The macrophages phagocytize fragments of disintegrating amelocytes. In addition to the well known complement of organelles the macrophages present an elaborated microtubular system, scattered, thick filaments, a cortical feltwork of thin filaments, and spherical nuclear bodies. The microtubules emanate from “attached” and free pericentriolar satellites and radiate aster-like towards the cell surface or into pseudopods or curve along the nuclear surface for long distances, often related to nuclear constrictions. It is suggested that the microtubular system plays a prominent role in directional movement of the macrophages. The cortical filaments, if contractile, may create the cytoplasmic flow necessary for the cell motility.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00306907
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