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  • 1
    Electronic Resource
    Electronic Resource
    The contribution of solvent drag to the intestinal absorption of the acidic drugs benzoic acid and salicylic acid from the jejunum of the rat (1974)
    Springer
    Naunyn-Schmiedeberg's archives of pharmacology 281 (1974), S. 197-217 
    Details
    ISSN: 1432-1912
    Keywords: Intestinal Absorption ; Acidic Drugs ; Benzoic Acid ; Salicylic Acid ; Solvent Drag ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary 1. Jejunal loops of anaesthetized rats were perfused with hypotonic, isotonic and hypertonic buffered solutions containing 14C-labelled benzoic acid and salicylic acid at pH 6.2 and 2.2. The blood flow of the loop was maintained at an intermediate rate (0.78–0.97 ml min−1 g−1). The water net flux was determined by polyethylene glycol as non-absorbable marker and amounted up to +31 or-27 μl min−1 g−1. 2. A positive water net flux (leaving the gut lumen) increased the appearance rate of benzoic acid and salicylic acid in the intestinal venous blood by maximally 47 and 41%, a negative water net flux (entering the gut lumen) diminished the appearance rate by 28 and 37%. 3. The experimental data were analysed by means of a kinetic model. The parameters of the model were the epithelial permeability kF D , the serosal permeability k S F S and the sieving coefficient Φ=1−σ. The epithelial permeability was 0.115 and 0.107 for benzoic acid and salicylic acid at pH 6.2 and increased to 0.163 and 0.185 ml min−1 g−1 at pH 2.2. The serosal permeability was assumed to be identical for neutral and acidic pH and amounted to 0.046 and 0.112 ml min−1 g−1 indicating that a certain proportion of the absorbed drugs was transferred to the serosal side. The sieving coefficient was 2.92 and 2.31 at neutral pH and 3.79 and 3.56 at acidic pH. 4. The main resistance to the absorption of the two drugs is the epithelial membrane, since the effective blood flow and the unstirred layer are not ratelimiting. The high sieving coefficient for the two drugs is interpreted as indicating an interaction of drug and water molecules inside the lipid part of the cell membrane (which contains at least 30% water). The electric potential across the gut wall may contribute to the size of the sieving coefficient, if the drug molecules permeate also in the ionized form. 5. Using acidic perfusion solutions the epithelial permeability and the sieving coefficient were increased by the factor 1.3–1.5. This increase can be explained sufficiently by a facilitated entrance of the unionized drug molecules into the epithelial membrane.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00503498
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  • 2
    Electronic Resource
    Electronic Resource
    Dekorporierung von Thallium durch rhyolithischen Bims bei der Ratte (1974)
    Springer
    Research in experimental medicine 162 (1974), S. 63-66 
    Details
    ISSN: 1433-8580
    Keywords: Rhyolithic pumice ; Ferrihexacyanoferrate (II) ; Thallium ; Rat ; Rhyolithischer Bims ; Ferrihexacyanoferrat (II) ; Thallium ; Ratte
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Orale Verabfolgung von rhyolithischem Bims bewirkt eine herabgesetzte Retention von Thallium in den Geweben. Die Wirksamkeit von Rhyolith zeigt eine ausgeprägte Dosisabhängigkeit, ist aber geringer als die von Ferrihexacyanoferrat (II).
    Notes: Summary Oral administration of rhyolith leads to a diminished retention of thallium by the tissues. The effectiveness of rhyolith markedly depends on its dosage, but is surpassed by that of ferrihexacyanoferrate (II).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01851884
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  • 3
    Electronic Resource
    Electronic Resource
    Untersuchungen zur Beeinflussung der renalen Exkretion von D-Penicillamin bei der Ratte (1974)
    Springer
    Research in experimental medicine 163 (1974), S. 39-45 
    Details
    ISSN: 1433-8580
    Keywords: D-penicillamine ; Acetazolamide ; Renal excretion ; Rat ; D-Penicillamin ; Acetazolamid ; Renale Exkretion ; Ratte
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Die renale Ausscheidung von D-Penicillamin läßt sich weder durch Probenecid noch durch N-Methylnicotinamid oder NH4Cl beeinflussen. Acetazolamid bewirkt eine erhöhte Retention von D-Penicillamin in Haut und Muskulatur und hemmt dessen renale Ausscheidung, hat jedoch keinen Effekt auf die Dekorporationswirksamkeit von D-Penicillamin bei60Co. Die möglichen Ursachen des Einflusses von Acetazolamid auf die Verteilung und Exkretion von D-Penicillamin werden diskutiert.
    Notes: Summary The renal excretion of D-penicillamine is not influenced by probenecid, N-methylnicotinamide, and NH4Cl. Acetazolamide brings about an increased retention of D-penicillamine in skin and muscles and lowers its urinary excretion, but does not exhibit any influence on the efficacy of D-penicillamine in removing internally deposited60Co. The possible causes for the action of acetazolamide on the distribution and excretion of D-penicillamine are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01851444
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  • 4
    Electronic Resource
    Electronic Resource
    Experimental uremic syndrome in the rat (1974)
    Springer
    Research in experimental medicine 163 (1974), S. 341-349 
    Details
    ISSN: 1433-8580
    Keywords: Experimental uremic syndrome ; Rat ; 5/6 reduction of renal parenchyma ; Serum- and urine analysis ; Secondary hyperparathyroidism ; Experimentelles urämisches Syndrom ; Ratte ; 5/6-Reduktion des Nierenparenchyms ; Serum- und Urinanalyse ; Sekundärer Hyperparathyreoidismus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Durch eine 5/6-Nierenparenchymreduktion wurde an Ratten ein chronisches, durch bilaterale Nephrektomie ein akutes urämisches Syndrom erzeugt. Beim chronischen urämischen Syndrom erwiesen sich in der Serumanalyse die Konzentrationen des Harnstoffstickstoffs und des Kreatinins als wesentlich erhöht. Darüber hinaus bestanden alle chemischen Zeichen eines sekundären Hyperparathyreoidismus. Die Tiere litten außerdem an einer schweren Poly-, Protein- und Albuminurie. Überraschenderweise war aber der Spiegel des totalen Serumproteins gegenüber den Kontrolltieren erhöht. Das experimentelle chronische urämische Syndrom stellt eine extreme Adaptation der regulatorischen Aktivität des Nierenrestparenchyms und des ganzen Organismus dar. Beim akuten, durch bilaterale Nephrektomie erzeugten urämischen Syndrom besteht dagegen ein schweres Versagen der Regulation des Wasser- und Elektrolytstoffwechsels, welches innert 50–60 Std zum Tode der Versuchstiere führt.
    Notes: Summary A chronic uremic syndrome was induced by 5/6 nephrectomy and an acute uremic syndrome by bilateral nephrectomy in rats. In the chronic uremic syndrome serum and urine analysis revealed a highly increased serum level of BUN and creatinine. Furthermore, all chemical signs typical of secondary hyperparathyroidism were present. The animals suffered from severe polyuria, protein- and albuminuria but, surprisingly, the total serum protein was increased. The experimental chronic uremic syndrome is, in essence, a syndrome of “extreme adaptation” of the regulatory activity of remnant renal parenchyma and of the whole body. In contrast, the acute uremic syndrome induced by bilateral nephrectomy is a severe disorder of water and electrolyte metabolism resulting in death of the experimental animals after 50–60 hrs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01851521
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  • 5
    Electronic Resource
    Electronic Resource
    Ultrastruktur des proximalen Nierentubulus nach Behandlung mit einem Pentosanschwefelsäureester (1974)
    Springer
    Research in experimental medicine 164 (1974), S. 19-33 
    Details
    ISSN: 1433-8580
    Keywords: Kidney ; Rat ; Ultrastructure ; Proximal tubule degeneration ; Heparinoid ; Niere ; Ratte ; Ultrastruktur ; Proximale Tubulusdegeneration ; Heparinoid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung 24 Std nach i.v. Injektion von 100 mg/kg eines Pentosanschwefelsäureesters (SP 54) sind in der Nierenrinde von Ratten schwere degenerative Veränderungen der proximalen Tubuluszellen zu beobachten. Das Tubulusepithel ist flachkubisch umgewandelt, das Tubuluslumen ist angefüllt mit ausgestoßenen Mitochondrien und anderen Zelltrümmern. Am stärksten betroffen ist die Pars contorta des proximalen Tubulus. Weniger stark veränderte Tubuluszellen sind angefüllt mit Cytosomen, die eine spezifische Feinstruktur haben oder gehäuft gegenüber Kontrollen Cytoplasmaprotrusionen in das Tubuluslumen aufweisen. Die akute Tubulusdegeneration ist ein spezifischer Effekt von SP 54. Heparin bewirkt nur eine leichte Schwellung des Tubulusepithels, die häufiger auftritt als bei Kontrolltieren.
    Notes: Summary 24 hrs after the intravenous injection of 100 mg/kg of a Pentosansulfuricacidester (SP 54) severe degenerative alterations are observed in the proximal tubular cells in rat kidney cortex. The tubular epithelium has changed to a single layer of elongated cubic cells. The tubular lumen is filled with cellular debris and extruded mitochondria. The greatest change is seen in the pars contorta of the proximal tubule. Less degenerated tubular cells have a great number of cytosomes with a specific substructure or have cytoplasmatic protrusions into the tubular lumen. This acute tubular degeneration is a specific event after the administration of SP 54 and is not seen after an equimolar dosis of Heparin. Heparin induces only a slight swelling of the tubular epithelium, more frequent seen as in the control animals.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01851961
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  • 6
    Electronic Resource
    Electronic Resource
    Influence of coprophagy prevented by partial immobilization on calcium metabolism and bone morphology in the rat (1974)
    Springer
    Research in experimental medicine 162 (1974), S. 313-321 
    Details
    ISSN: 1433-8580
    Keywords: Coprophagy ; Rat ; Calcium metabolism ; Bone morphology ; Immobilization ; Koprophagie ; Ratte ; Calciumstoffwechsel ; Knochenmorphologie ; Immobilisierung
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Wir untersuchten den Einfluß der Koprophagie auf den Calciumstoffwechsel der Ratte. 20 Versuchstiere wurden durch Einschnüren in einen Lederpanzer an der Koprophagie gehindert und mit 21 Kontrolltieren gleicher Futteraufnahme verglichen. Die Kinetik des Calciumstoffwechsels wurde unter Verwendung von45Ca als Tracer analysiert. Die radiochemischen Resultate wurden mit den histologischen Beobachtungen an den Epi-Metaphysen der Kniegelenke verglichen. Bei den Versuchstieren mit verhinderter Koprophagie fanden wir: 1. ein hochsignifikant niedrigeres Körpergewicht; 2. eine hochsignifikante Verkleinerung folgender Parameter des Calciumstoffwechsels: Compartmentgröße und Austauschrate zwischen ihnen, Calciumausscheidungsraten, Knochenan- und -abbau; 3. eine Verschmälerung der Wachstumsfuge, verkürzte und verschmälerte primäre Knochenbälkchen, verminderte sekundäre metaphysäre Spongiosa. Bei 4 Versuchstieren, die in Lederpanzern gehalten, denen jedoch die Koprophagie ermöglicht war, wurden gleichartige histologische Veränderungen wie bei verhinderter Koprophagie festgestellt. Es wird gefolgert, daß die Verhinderung der Koprophagie keinen meßbaren Einfluß auf die Parameter des Calciumstoffwechsels hat.
    Notes: Summary We investigated the influence of coprophagy on the calcium metabolism of the rat. 20 experimental animals were prevented from coprophagy by wrapping them in leather harnesses and compared with 21 controls on the same food intake. Kinetics of calcium metabolism were analysed using45Ca as tracer. The radiochemical results were compared with the histological observations in the epi-metaphyses of the knee joints. In the experimental animals prevented from coprophagy we found: 1. a highly significant lower body weight; 2. a highly significant diminution of the following parameters of calcium metabolism: compartment sizes and exchange rate between them, calcium excretion rates, bone accretion and resorption; 3. a narrower epiphyseal growth cartilage, clearly shorter and narrower primary trabeculae, rarefied secondary metaphyseal trabeculae. Histological alterations identical to those observed with coprophagy prevented, were found in 4 animals kept in harnesses but with coprophagy allowed. It is concluded that prevention of coprophagy has no measurable influence on the parameters of calcium metabolism.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01851702
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  • 7
    Electronic Resource
    Electronic Resource
    Wirkung steigender Pentagastrindosen auf die Säuresekretion des Rattenmagens nach Vagotomie im Perfusionsversuch (1974)
    Springer
    Research in experimental medicine 163 (1974), S. 289-298 
    Details
    ISSN: 1433-8580
    Keywords: Gastrinpentapeptide ; Vagotomy ; Acid secretion ; Perfusion test ; Rat ; Gastrinpentapeptid ; Vagotomie ; Säuresekretion ; Perfusionsversuch ; Ratte
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Bei männlichen Wistarratten wurde eine trunkuläre beiderseitige Vagotomie — ergänzt durch eine Pyloroplastik — angelegt. 4 Wochen später erfolgte in Urethannarkose eine Perfusion des Magens mit 0,9%iger NaCl-Lösung in Anlehnung an die Versuchsanordnung von Ghosh. Die Vollständigkeit der Vagotomie bestätigte ein Hypoglykämietest. Im Abstand von 150 min erhielten die Tiere — in 6 Gruppen zu je 10 Ratten — 2 × 50, 100, 150, 200, 250 oder 300 µg/kg KG Pentagastrin subcutan. Die statistisch ausgewerteten Ergebnisse lassen sich wie folgt zusammenfassen: 1. Die nach dem Abklingen der Wirkung der ersten Pentagastrininjektion wiederholte Pentagastringabe in derselben Dosis ergab bei allen Dosierungen eine höhere Säuresekretion als nach der ersten Injektion. 2. Ein Maximum der sezernierten Säuremenge wurde bei einer Dosis von 100 µg/kg KG erreicht. Bei höherer Dosierung des Pentagastrins nahm die Menge der sezernierten Säure signifikant ab.
    Notes: Summary Male Wistar rats were subjected to a complete bilateral vagotomy and a pyloroplasty. 4 weeks later followed in urethane anesthesia a continuous perfusion of the stomach with 0.9% saline according to the rat preparation of Ghosh. The completeness of the gastric vagotomy was confirmed by a postoperative insulin test. In an interval of 150 min the rats — in 6 groups of 10 rats each — were two times injected pentagastrin subcutaneously in dose rates of 50, 100, 150, 250 or 300 µg/kg body weight. The statistical confirmed results can be summarized as followed: 1. The second injection of pentagastrin — 150 min after the first — in the same dose produced a significantly higher gastric acid secretion in the rat all dose rates in comparison to the first injection. 2. The maximal gastric acid secretion was produced by a dose rate of 100 µg/kg body weight of pentagastrin. At higher dose rates of pentagastrin there was a significant reduction of gastric acid secretion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01851516
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  • 8
    Electronic Resource
    Electronic Resource
    Functional changes of the neuronal and glial elements at the surface of the external layer of the median eminence (1974)
    Springer
    Anatomy and embryology 143 (1974), S. 255-262 
    Details
    ISSN: 1432-0568
    Keywords: Median eminence ; Neuro-vascular contacts ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The vascular surface of the external layer of the median eminence is composed of neuronal and glial processes. Neuro-vascular contacts are supposed to be necessary for the release of hypophysiotropic neurohormones. The present study confirms the hypothesis that the relative extent of neuro-vascular and glio-vascular contacts varies under different functional conditions of the tubero-hypophysial system. Morphometric analysis shows that in normal rats about 20% of the surface is lined by neuro-vascular contacts. After bilateral adrenalectomy the neuro-vascular contacts are enlarged to 40% whereas application of an extract of stalk-median eminence tissue is followed by a decrease of the neuro-vascular contacts to 15%. The implications of these results are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00519869
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  • 9
    Electronic Resource
    Electronic Resource
    Über die Entstehung der Basalmembranlabyrinthe (1974)
    Springer
    Anatomy and embryology 143 (1974), S. 185-203 
    Details
    ISSN: 1432-0568
    Keywords: Basement membrane labyrinths ; Cerebral ventricles ; Development ; Rat ; Light and Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Gehirne embryonaler und neugeborener Ratten wurden im Hinblick auf die frühe Entstehung der Basalmembranlabyrinthe licht- und elektronenmikroskopisch untersucht. 1. An den Hirngefäßen embryonaler Ratten der 2. und 3. Fetalwoche kommen zwar bereits Basalmembranen vor, sub- oder interependymale Basalmembranlabyrinthe fehlen aber noch. 2. Basalmembranlabyrinthe werden mit der Perjodsäure-Bisulfit-Aldehydthionin-Methode lichtmikroskopisch ab dem 20. Tag an umschriebenen Stellen des Ventrikelsystems gefunden; elektronenmikroskopisch treten die ersten Basalmembranlabyrinthe am 12. postnatalen Tag auf. 3. Die ersten interependymalen Labyrinthe erscheinen in Erweiterung der interependymalen Spalten in der Nähe von ausgedehnten Golgifeldern. Der Labyrinthinhalt besteht aus einer feinflockigen Substanz. Diese liegt wolkenartig angehäuft gegenüber bogenförmigen Ausbuchtungen lokal verbreiterter Zellmembranen. In Ausbildung begriffene Labyrinthe zeigen eine lamelläre Gliederung. 4. Ab dem 12. postnatalen Tag treten auch an den Basalmembranen der pericapillären Zellen, in denen lichtmikroskopisch Glykoproteide nachweisbar sind, zapfenartige Duplikaturen auf. Die Zapfen sind überwiegend ependymwärts gerichtet. Verbindungen zwischen den Basalmembranzapfen und den interependymalen Basalmembranlabyrinthen werden in den ersten 30 Lebenstagen nicht gefunden. 5. Sowohl an den die interependymalen Labyrinthe begrenzenden Plasmalemmata als auch am Plasmalemm der pericapillären Zellen fallen Stachelsaumbläschen auf, deren Membran in die Zellwand eingebaut wird. Ihr Inhalt scheint in die entstehenden Labyrinthe entleert zu werden. 6. Da sich an den tubulären Enden der Doppellamellen der Dictyosomen Strukturen befinden, die den Stachelsaumbläschen morphologisch gleichen, und da im Bereich des Golgifeldes und im Cytoplasma zwischen diesem und der Zellwand zahlreiche Stachelsaumbläschen liegen, wird angenommen, daß das Material der Basalmembranlabyrinthe dem Golgiapparat entstammt und vermittels der Stachelsaumbläschen zur Zellwand transportiert wird. Bogenförmige Ausbuchtungen lokal verbreiterter Zellmembranen im Bereich entstehender Labyrinthe werden als Reste von Stachelsaumbläschen aufgefaßt, deren Membran in die Zellwand eingebaut wurde. 7. Da die phylogenetische Zunahme des Hirnmantels mit einem Verlust der langen in das Gehirn reichenden basalen Ependymfortsätze an den größten Teilen der Ventrikelwand einhergeht und niedere Tiere noch nicht über Basalmenbranlabyrinthe verfügen, wird angenommen, daß diese die Transportfunktion für Stoffe aus dem Liquor übernommen haben, die vordem nach allgemeiner Auffassung über die langen Fortsätze in das Gehirn gelangten.
    Notes: Summary The brains of embryonic and new-born rats were investigated by means of light- and electron microscopy with regard to the early formation of basement membrane labyrinths. 1. Though basement membranes are already found around the brain capillaries of embryonic rats from the 2nd week of pregnancy, sub- and interependymal basement membrane labyrinths are still absent. 2. Basement membrane labyrinths, being demonstrable for light microscopy by a periodicacid-bisulfite-aldehydethionin-method, appear around the 20th day after birth at certain places of the ventricular system. By means of electron microscopy, basement membrane labyrinths have first been detected at the 12th postnatal day. 3. The earliest interependymal basement membrane labyrinths are found in enlargements of the interependymal spaces near a distended Golgi apparatus. The contents of the labyrinths, being composed of a loose flocculent material, are of a lamellar structure. In the intercellular space the material is situated opposite bow-shaped excavations of local broadenings of cell membranes. 4. From the 12th postnatal day, plug-like duplications of basement membranes occur at the ependymal side of the pericapillar cells, which contain glycoproteids. The plugs of basement membranes are directed towards the ependymal layer. No connections between the interependymal basement membrane labyrinths and the plugs of pericapillary basement membranes exist within the first 30 days of life. 5. At the plasmalemma of ependymal cells bordering the interependymal labyrinths, and at the cell membrane of pericapillary cells, coated vesicles are to be found, which are fused with the cell membrane. The contents of these vesicles seem to be released into the developing labyrinths. 6. At the tubular ends of dictyosomes, coated vesicle-like structures can be demonstrated. In the environment of the Golgi apparatus many coated vesicles are situated; they can even be found between the Golgi apparatus and the walls of labyrinthś. Therefore the coated vesicles are considered to be transport vesicles, transporting the material which is formed in the Golgi apparatus towards the cell membrane. 7. Since the phylogenetic increase of the brain mantle is accompanied by loss of the long processes of ependymal cells that reach far into the brain, and since lower animals have no basement membrane labyrinths, it is suggested that the basement membrane labyrinths have a transport functions for material from the cerebrospinal fluid which in lower animals is assumed to be transported by the long processes of ependymal cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00525769
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  • 10
    Electronic Resource
    Electronic Resource
    Feinbau und Passageverhalten der Capillaren im Subcommissuralorgan der Ratte (1974)
    Springer
    Anatomy and embryology 144 (1974), S. 101-122 
    Details
    ISSN: 1432-0568
    Keywords: Subcommissural organ ; Periodically structured bodies ; Vascular permeability ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Die Capillaren des Subcommissuralorgans (SCO) und deren Umgebung wurden an 38 adulten Sprague-Dawley- und an 6 adulten Wistar-Ratten nach Perfusionsfixierung im Elektronenmikroskop untersucht. Saure Mucopolysaccharide wurden mit Alcianblau und Rutheniumrot, Glykoproteide mittels der Perjodsäure-Silbermethenamin-Reaktion nachgewiesen. Der Stofftransport in den Capillaren wurde mit Meerrettichperoxidase (MRP) als Tracer untersucht. Die subcommissuralen Capillaren besitzen ein ungefenstertes, vesikelarmes Endothel, das einer einfachen, durchgehenden Basallamina aufsitzt. In der Umgebung der Capillaren finden sich neben periodisch strukturierten Körpern (PSK) vereinzelt Basallamina-Labyrinthe und regellos angeordnete kollagene Mikrofibrillen. Mit der Perjodsäure-Silbermethenamin-Reaktion lassen sich Glykoproteide im Bereich der Basallamina und der nach außen anschließenden Intercellularspalten darstellen, vereinzelt auch — teils im Bereich der Basallamina, teils ihr eng angeschlossen — Gruppen parallel angeordneter, linearer Strukturen mit einem periodischen Streifenmuster. Saure Mucopolysaccharide sind nur in der lumenseitigen Glykokalyx des Capillarendothels nachweisbar. Der Aufbau der PSK aus Kollagenfilamenten erscheint sehr wahrscheinlich. Vermutungen zur Entstehung dieser hochgeordneten Strukturen werden vorgebracht. Die Traceruntersuchungen ergeben, daß unter normalen Bedingungen in den subcommissuralen Capillaren eine Blut-Hirn-Schranke (BHS) für MRP besteht, die im Endothel lokalisiert ist. Bei einem Teil der Sprague-Dawley-Ratten ist die Barriere geschädigt und der Tracer gelangt über cytopemptische Vesikel in Basallamina und Gefäßumgebung. Der Basallamina und den PSK kommt in solchen Fällen keine besondere Schranken- oder Verteilerfunktion zu. Offenbar bewirkt bei manchen Sprague-Dawley-Ratten die MRP-Injektion über die Freisetzung von endogenen biogenen Aminen aus Mastzellen eine Schädigung der BHS, was bei Wistar-Ratten nicht eintritt.
    Notes: Summary The capillaries of the subcommissural organ (SCO) and their adjacent structures of 38 adult Sprague-Dawley rats and 6 adult Wistar rats were examined in the electron microscope after perfusion fixation. Acid mucopolysaccharides were shown by Alcian Blue and Ruthenium Red, glycoproteins were identified by the periodic acid-silver methenamine technique. Horseradish peroxidase (MRP) was used as a tracer for vascular permeability. The subcommissural organ's capillaries are characterized by an unfenestrated, continuous endothelium, showing only few vesicles; the endothelium is surrounded by a single, continuous basal lamina. Adjacent to the capillaries wall there are “periodically structured bodies” (PSK), labyrinths of the basal lamina and irregularly arranged collagen fibrils. Glycoproteins are found in the basal lamina and within the intercellular clefts; in a few cases groups of parallel arranged, periodically banded linear structures are seen within or adjacent to the basal lamina. Acid mucopolysaccharides are found within the endocapillary layer of capillaries. It seems very likely that PSK represent an atypical arrangement of collagen fibrils. The formation of these structures is discussed. Under normal conditions, MRP, after intravenous injection, does not enter the perivascular space surrounding the capillaries of the SCO. A blood-brain barrier exists at the level of the capillary endothelium. In a few Sprague-Dawley rats MRP seems to alter the permeability of the capillaries. Reaction product of MRP is found within endothelial vesicles and the adjacent structures. It appears that the basal lamina and the PSK are not involved in the barrier mechanism or regulation of transport. In Sprague-Dawley rats intravenous injection of MRP seems to alter the blood-brain barrier by release of endogenous biogenic amines associated with mast cell degranulation. This effect is not observed in Wistar rats.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00518636
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  • 11
    Electronic Resource
    Electronic Resource
    Changes of extent of Neuro-vascular contacts and number of neuro-glial synaptoid contacts in the pituitary posterior lobe of dehydrated rats (1974)
    Springer
    Anatomy and embryology 146 (1974), S. 157-165 
    Details
    ISSN: 1432-0568
    Keywords: Neurohypophysis ; Neuro-vascular contacts ; Neuro-glial synaptoid contacts ; Dehydration ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The posterior lobe of the hypophysis consists of neurosecretory nerve fibres and glial cells, both abutting on the perivascular space of sinusoidal capillaries. Nerve fiberes and glial cells are connected with each other by a high number of synaptoid contacts. Several ultrastructural changes following dehydration have been described by other authors. This study presents the data of some morphometric investigations on normal and dehydrated rats. The relative extent of neuro-vascular contacts is not contstant; it increases significantly from 52 (±2.8)% in normal rats to 67(±1.2)% in rats with a dehydration time of 3 days. Correspondingly the extent of glio-vascular contacts is diminished. In addition, water-deprived animals show a clear increase in the number of neuro-glial synaptoid contacts with 3.1 (±0.4) per square unit nervous tissue compared to 2.1 (±0.3) per Square unit in untreated controls. These results indicate a dynamic motility of neuronal and glial elements depending on functional conditions, as was found for the external layer of the median eminence. Furthermore there seems to be a relationship between secretory activity and the number of neuroglial synaptoid contacts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00315592
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  • 12
    Electronic Resource
    Electronic Resource
    Electron microscopic studies of the innervation of the rat kidney (1974)
    Springer
    Anatomy and embryology 145 (1974), S. 169-186 
    Details
    ISSN: 1432-0568
    Keywords: Renal innervation ; Rat ; Peripheral nerves ; Neuroeffector zones ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung In der Rattenniere werden die muskelzellhaltigen arteriellen Gefäße und der juxtaglomeruläre Apparat innerviert. Blutgefäße mit Pericyten, porenhaltige Capillaren sowie die Tubuli der Rinde und des Markes werden nicht von Nervenfasern begleitet. Ganglienzellen wurden in der Rattenniere nicht beobachtet. Periphere Nerven mit einem ein-bis zweischichtigen Perineurium kommen im paravasalen Gewebe der Interlobar- und Arcuata-Arterien vor; sie enthalten neben zahlreichen marklosen Nervenfasern gewöhnlich auch 2–4 markhaltige. Nervenfaser-Bündel ohne perineurale Scheide finden sich im paravasalen Gewebe der Arcuata- und Interlobular-Arterien. Darüber hinaus sind in unmittelbarer Nachbarschaft der großen Arterien (Interlobar-, Arcuata- und Inter-lobular-arterien) und der Vasa afferentia marklose Nervenfasern und freie Axone vorhanden, die auch die proximalen Abschnitte der Vasa efferentia der subcapsulären und intermediären Rindenschicht begleiten. Im Nierenmark werden die juxtamedullären Vasa efferentia und die Arteriolae rectae innerviert; marklose Nervenfasern und freie Axone sind nur bis zur Außen-Innenstreifen-Grenze nachweisbar. Die Innervation der muskelzellhaltigen arteriellen Gefäße erfolgt durch aufgetriebene Axonabschnitte (Neuroeffektor-Zonen), die vorwiegend agranuläre Vesikel enthalten. Diese Strukturen liegen stets an der Grenze von Adventitia und Media bzw. Elastica externa; zwischen den glatten Muskelzellender Media wurden keine vesikelhaltigen Axonabschnitte gefunden. Als minimaler Abstand zwischen den vesikelhaltigen Axonabschnitten und den von einer Basalmembran umschlossenen glatten Muskelzellen (neuromuskuläre Distanz) wurden 600 Å gemessen. Die vorliegenden Ergebnisse werden mit den fluoreszenzmikroskopischen und histochemischen Untersuchungen über die adrenerge und cholinerge Innervation der Niere verglichen. Die sich aus diesem Vergleich ergebenden Probleme und funktionellen Konsequenzen für die Innervation der Niere sowie die Natur der cholinergen Fasern (afferente oder postganglionäre parasympathische Fasern) werden diskutiert.
    Notes: Summary The innervation of the rat kidney is defined by a system which supplies those arterial blood vessels whose walls contain smooth muscle cells and the juxtaglomerular apparatus. Vessels containing pericytes, or those vessels composed of an endothelium only, as well as the tubules of both the cortex and medulla, are not innervated. Furthermore, ganglion cells do not occur in the rat kidney. The nervous apparatus of the rat kidney consists of peripheral vegetative nerves, ensheathed by a perineurium, with 2–4 myelinated fibers running in the paravasal tissue of the interlobar and arcuate arteries, and of nerve bundles without a perineurial sheath in the paravasal tissue of the arcuate and interlobular arteries. Non-myelinated fibers and free axons occur in the immediate vicinity of the great arteries (interlobar, arcuate, and interlobular) and the vasa afferentia. Nerve fibers and free axons are also seen in the vicinity of only the proximal parts of those vasa efferentia which supply the cortical capillary plexus. The arteriolae rectae of the medulla, and their vasa efferentia, from which they arise, are innervated by non-myelinated fibers and free axons which accompany these arterial vessels only to the boundary of the outer and inner stripe of the outer zone of the medulla. The functional innervation of those vessels with smooth muscle cells results from neuro-effector zones which predominantly show agranular vesicles. These structures were never seen between the smooth muscle cells within the media; the minimum neuromuscular distance was 600 Å. The present findings are correlated with the lightmicroscopically demonstrated adrenergic and cholinergic innervation. The resultant problems and functional consequences of the innervation of the kidney, especially the nature of the cholinergic fibers (afferent or post-ganglionic parasympathetic fibers) are briefly discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00519727
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  • 13
    Electronic Resource
    Electronic Resource
    Neuroembryogenesis in the hippocampal formation of the rat (1974)
    Springer
    Anatomy and embryology 144 (1974), S. 173-186 
    Details
    ISSN: 1432-0568
    Keywords: Hippocampus ; Neurogenesis ; Autoradiography ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summay The prenatal ontogeny of the hippocampus, including the anterior continuation and induseum griseum, was examined in the rat by means of tritiated thymidine autoradiography. The neurons of the anterior continuation formed between the 16th and 18th days of embryonic development. The neurons of the induseum griseum were formed on the 16th and 17th days of gestation. The pyramidal cells of ammon's horn were formed during the 16th, 17th, 18th, and 19th days of embryogenesis but were preceded by the large interneurons of the stratum lacunosum-moleculare which formed on days 15 and 16 of embryonic life. The granule cells of the superficial layers of the stratum granulosum formed during the 20th, 21st, and 22nd days of gestation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00519773
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  • 14
    Electronic Resource
    Electronic Resource
    Morphological types of motor nerve terminals in rat hindlimb muscles, possibly innervating different muscle fiber types (1974)
    Springer
    Anatomy and embryology 144 (1974), S. 237-247 
    Details
    ISSN: 1432-0568
    Keywords: Motor nerve terminals ; Methylene blue stain ; Hindlimb muscles ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary With methylene blue stain, three morphological types of motor nerve terminals were distinguished in hindlimb muscles (extensor digitorum longus, semitendinosus, soleus, and tibialis anterior muscles) of rat. Type A terminals possess many long thin branches with numerous minute swellings, type C ones possess few and thick terminal branches with few large swellings, and type B ones possess intermediate characteristics. The frequencies of occurrence of these types are characteristic for each muscle and differ between different hindlimb muscles. Specifically, soleus muscle contains a much higher proportion of type B terminals than other hindlimb muscles, and the superficial part of tibialis anterior muscle contains a higher proportion of type A terminals and a lower proportion of type C ones than the deep part. The frequencies of occurrence of types A, B, and C terminals closely parallel the frequencies of “white”, “intermediate”, and “red” muscle fibers (as determined by a fat stain), respectively, in the different muscles. As also suggested previously for the diaphragm, it is concluded that three morphological types of motor nerve terminals each may innervate one of three types of muscle fibers.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00522809
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  • 15
    Electronic Resource
    Electronic Resource
    Influence of pantothenic acid deficiency on the amount of CRF-granules in the rat median eminence (1974)
    Springer
    Anatomy and embryology 146 (1974), S. 43-55 
    Details
    ISSN: 1432-0568
    Keywords: Pantothenic acid deficiency ; Corticotropin-releasing factor ; Corticoids ; Histologie ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In the outer layer of the rat median eminence “Gomori-positive” granules occur which are assumed to be the morphological correlate of the corticotropin-releasing factor (CRF). The present study was carried out in order to establish whether the amount of these CRF-granules is influenced by a pantothenic acid (PA)-deficiency, which is known to result in an inhibition of corticosteroid production. Female SPF Wistar rats were used. PA-deficiency was induced by PA-free nutrition and/or administration of the PA-antagonist Ω-methyl-PA. Corresponding experimental groups were given either tap water or a solution of 1% NaCl and 5% glucose in tap water to drink. In rats fed with a diet free of PA and in addition treated with Ω-methyl-PA a severe disturbance of the corticoid balance developed within 6 weeks. The corticosterone production was depressed to about 11–16% of the control values. The adrenal cortex showed necrotic changes which either involved the whole parenchyma or were confined to the inner zones. In those animals in which a subcapsular region, in its extent corresponding to the zona glomerulosa, remained intact, an augmentation of the CRF-granules was observed. In those animals, however, in which the whole adrenal cortex was damaged, the amount of CRF-granules did not increase. Administration of the 1% NaCl containing drinking fluid did not influence the augmentation of the CRF granules, but had a protective effect on the development of necrotic changes in the zona glomerulosa of the adrenal cortex. The findings show that a functional relationship exists between the inner zones of the adrenal cortex and the CRF-granules in the median eminence.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00341382
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  • 16
    Electronic Resource
    Electronic Resource
    Scanning electron microscopy demonstration of cilia in rat intrahepatic bile ducts (1974)
    Springer
    Anatomy and embryology 145 (1974), S. 223-226 
    Details
    ISSN: 1432-0568
    Keywords: Rat ; Liver ; Bile ducts ; Cilia ; Scanning Electron Microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Numerous cilia have been demonstrated by SEM in cells of rat perilobular and portal bile ducts and ductules. Generally these cilia appear as long, cylindrical evaginations of the plasma membrane. Some of them are so long that, curving and twisting at many points, they cross the ductal lumen. It has been suggested that they may be related to a continual mixing up and propulsion of the bile product down the biliary tree.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00519634
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  • 17
    Electronic Resource
    Electronic Resource
    The fine structure of the pulmonary stretch receptor in the rat (1974)
    Springer
    Anatomy and embryology 143 (1974), S. 215-222 
    Details
    ISSN: 1432-0568
    Keywords: Lung ; Stretch receptors ; Light microscopy ; Electron microscopy ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Lappenbronchien (3./6.) (Durchmesser bei mittlerer Inspirationslage 500–700 μ) von männlichen und weiblichen Wistarratten wurden licht- und elektronenmikroskopisch untersucht. Diese Bronchien entsprechen im Wandbau den mittleren Bronchioli größerer Säugetiere. Freie, verzweigte lanzettförmige Terminalfasern werden im Bindegewebe der lamina propria beschrieben. Sie sind in das elastisch muskuläre System der Bronchuswand eingebaut. Die zugehörige markhaltige afferente Nervenfaser ist verzweigt und hat einen Durchmesser von 4–6 μ. Außerdem werden efferente motorische Endigungen an der glatten Bronchialmuskulatur beschrieben. Die freien lanzettförmigen und verzweigten Terminalfasern sind möglicherweise Dehnungsrezeptoren für den Hering Breuer Reflex.
    Notes: Summary In rats the 3rd to 6th bronchi, measuring 500–700 μ in diameter during inspiration, were investigated by light and electron microscopy. The histological appearance of these bronchi is comparable to that of medium sized bronchioles of larger animals. The branched and lanceolate terminals are associated with the connective tissue of the lamina propria and the smooth muscle cell layer. In this way the terminals are bound to the myoelastic system of the bronchial wall. The myelinated afferent fiber is branched and the diameter measures about 4–6 microns. Besides afferent nerve terminals these are numerous efferent endings on the smooth muscle basement laminae. It is supposed that the described receptor represents the pulmonary stretch receptor of the Hering Breuer reflex.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00525771
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  • 18
    Electronic Resource
    Electronic Resource
    Ganglienzellen und paraganglionäre Zellen des Ganglion cervicale superius der Ratte nach Parachlorophenylalanin (pCPA) (1974)
    Springer
    Anatomy and embryology 143 (1974), S. 223-237 
    Details
    ISSN: 1432-0568
    Keywords: Superior cervical ganglion ; Rat ; p-Chlorophenylalanine ; 5-Hydroxytryptamine ; Catecholamine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Die Wirkung von Parachlorophenylalanin (pCPA) auf die paraganglionären Zellen und sympathischen Ganglienzellen des Ganglion cervicale superius der Ratte wurde untersucht. pCPA beeinflußt die sympathischen Nervenzellen im Ganglion cervicale superius geringgradig. Es finden sich unspezifische Veränderungen des Golgi-Apparates und der Nissl-Schollen sowie eine Zunahme von Lysosomen. Bei langdauernder Behandlung degenerieren einzelne Ganglienzellen und Nervenfasern. Die paraganglionären Zellen sind bei Kontrolltieren durch “dense core vesicles” eines einheitlichen Typs charakterisiert. Im Laufe der pCPA-Behandlung finden sich degranulierte paraganglionäre Zellen. Die Degranulation der einzelnen Zellelemente ist jedoch variabel. Bei fluorescenzmikroskopischer Untersuchung zeigt sich nach pCPA-Applikation eine Verschiebung des Fluorescenzspektrums zum grünen Bereich hin. Die bei Kontrollen deutliche Gelbkomponente nimmt nach 6–12 Tagen ab und verschwindet schließlich nach 18–20 Tagen. Es wird diskutiert, ob die fluorescenzmikroskopischen Veränderungen aufgrund einer Hemmung der Serotoninsynthese oder aufgrund einer abgeschwächten Katecholaminsynthese entstehen.
    Notes: Summary The effect of parachlorophenylalanine (pCPA) on the paraganglionic and sympathetic nerve cells of the rat superior cervical ganglion was studied. pCPA influences the sympathetic nerve cells of the cervical ganglion to minimal extent. Unspecific changes of the Golgi apparatus and of the Nissl bodies as well as an increase in the number of lysosomes occur. An extended period of treatment causes degeneration of individual ganglionic cells and nerve fibers. The paraganglionic cells in control animals are characterized by dense core vesicles of an unitary type. During pCPA treatment degranulated paraganglionic cells are to be found. The degranulation of the individual cell elements varies. Fluorescence microscopic study after pCPA application shows a shift of the fluorescence spectrum toward green. The yellow component of the control group decreases after 6 to 12 days and disappears after 18 or 20 days. It is discussed whether the fluorescence microscopic changes are due to the inhibition of serotonine synthesis or to a decrease of the catecholamine synthesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00519867
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  • 19
    Electronic Resource
    Electronic Resource
    Effects of triparanol and 20,25-diazacholesterol in CNS of rat: Morphological and biochemical studies (1974)
    Springer
    Acta neuropathologica 29 (1974), S. 141-156 
    Details
    ISSN: 1432-0533
    Keywords: Cholesterol Inhibitors ; Cerebrum ; Neuronal Inclusions ; Oligodendroglial Degeneration ; Rat ; Abnormal Sterols
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Wistar strain of rats received daily intraperitoneal or oral administration of Triparanol, 250 mg per kg of body weight or 20,25-diazacholesterol either 30 mg or 60 mg per kg of body weight starting at the age of 5 days old. Prominent intracytoplasmic osmiophilic inclusions identical to those found in the rats treated with AY9944 (Suzukiet al., 1973) and degeneration of myelin sheaths and oligodendroglia were seen in the myelinating white matter of rats sacrificed after 3, 6, and 9 injections of Triparanol. In diazacholesterol-treated animals, a few identical inclusions were seen in the cortical neurons but only slight changes were seen in oligodendroglia. Biochemical analysis of the brains of the rats receiving Triparanol revealed a large accumulation ofΔ 7,24-cholestadiene-3β-ol and lesser amounts of desmosterol. The brains of the animals treated with diazacholesterol revealed massive accumulation of desmosterol and a minor sterol component, probablyΔ 5,7,24-cholestatriene-3β-ol. Since AY9944, which results in a massive accumulation of 7 dehydrocholesterol (Δ 5,7-cholestadiene-3β-ol) and two other sterols,Δ 7,24-cholestadiene-3β-ol andΔ 5,7,24-cholestatriene-3β-ol, will produce identical neuropathological lesions to those seen in Triparanol treated animals, and since the lesion produced in diazacholesterol treated animals was very slight, there appears to be a correlation between the presence of sterols with a double bond at the 7-position and neuropathological lesions in young rats.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00684773
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  • 20
    Electronic Resource
    Electronic Resource
    The morphology of experimental tumours of the nervous system (1974)
    Springer
    Acta neuropathologica 27 (1974), S. 25-32 
    Details
    ISSN: 1432-0533
    Keywords: Experimental CNS Tumours ; Nitrosomethylurea ; Ethylnitrosourea ; Primary Cultures ; Tumour Classification ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary By means of the explant technique a series of primary tumours induced by methylnitrosourea (MNU) or ethylnitrosourea (ENU) in the brain of rats were cultured in vitro. The morphology of isomorphic oligodendrogliomas, astrocytomas, glioblastomas and sarcomas under in vitro conditions is described. All these neoplasias show a very distinct morphological picture and growth behaviour, thus permitting their classification. Most of the tumours tend to anaplastic changes during longer cultivation, but in some cases, preferably in well differentiated gliomas, the cytological properties are maintained even during longterm cultivation. The role of anaplasia and intermingling of different neoplastic cell types in experimental tumours are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00687237
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  • 21
    Electronic Resource
    Electronic Resource
    Experimental allergic encephalomyelitis in the rat: Histopathological studies and the effects of sera on adult cerebellar organ cultures (1974)
    Springer
    Acta neuropathologica 27 (1974), S. 83-91 
    Details
    ISSN: 1432-0533
    Keywords: Allergic Encephalomyelitis ; Organ Culture ; Brain ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Experimental allergic encephalomyelitis (EAE) developed in 15 of 30 rats injected with a mixture of Freund's adjuvant and homogenized guinea pig brain, and killed after 6–29 days. The incidence and severity of the disease increased with time after inoculation and in 2 animals involvement of the epiphysis, not previously reported in EAE, was detected. Toxic effects on cells in cultured adult rat cerebellar cortical tissue were produced by exposure for 24 h to the sera of rats killed 12–29 days after inoculation either with the antigenic suspension or with adjuvant alone. Severe toxicity to axons (widespread fragmentation) was produced only by the sera of animals injected with antigenic inocula and killed 17–29 days later. Severe axonal toxicity was not, however, correlated with the presence of EAE in the rats from which the sera were taken. It was not possible to assess myelinotoxic effects in the system used. It is suggested that the organ culture technique may detect the presence in the sera of inoculated animals of toxic principles which, owing to the blood-brain barrier, do not normally contribute to the pathogenesis of EAE.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00687243
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  • 22
    Electronic Resource
    Electronic Resource
    Ultrastructural alterations of rat brain microglial cells and pericytes after chronic lead poisoning (1974)
    Springer
    Acta neuropathologica 28 (1974), S. 25-35 
    Details
    ISSN: 1432-0533
    Keywords: Chronic Lead Intoxication ; Rat ; Ultrastructure ; Microglia ; Pericytes ; Monocytes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Wistar rats of both sexes and the same litter were exposed to chronic lead intoxication from birth untill sacrifice 9 months later. Lead was administered as 0.4% solution of lead nitrate in drinking water. Samples from the parietal brain cortex were examined electron microscopically following intracardiac perfusion with paraformaldehyde-glutaraldehyde solution. Similar changes were observed in the microglial cells and the vascular pericytes whereas all the other tissue elements appeared intact. Both cell types hypertrophied, the microglia assumed characteristic spindle or rod shape, the cell organelles increased, the microglial endoplasmic reticulum widened strongly and a large number of lipid inclusions appeared. The latter consisted of large lipid droplets of varying size and shape, containing multiple zones of low density, and a dense component with a coarse granular structure. The similarity in the response of both cell entities to the lesion as well as some probable functions of microglial cells and their relationship to vascular pericytes are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00687515
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  • 23
    Electronic Resource
    Electronic Resource
    The effects of sodium fluoride on bone breaking strength (1974)
    Springer
    Calcified tissue international 14 (1974), S. 283-289 
    Details
    ISSN: 1432-0827
    Keywords: Sodium fluoride ; Osteoporosis ; Bone ; Strength ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Description / Table of Contents: Résumé L'utilisation thérapeutique de fluorure de sodium a été recommandée dans le traitement de diverses maladies osseuses ostéopéniques. Cet emploi est basé principalement sur les effects ostéosclérotiques bien connus du fluorure de sodium, mais on connait mal son effet sur la force de résistance osseuse. L'influence de diverses concentrations de fluorure de sodium sur la force de résistance osseuse de jeunes rats soumis à des régimes riches et pauvres en calcium a été étudiée. L'administration de fluorure de sodium augmente le diamètre osseux, indiquant une stimulation de la formation de l'os périosté, mais la force de résistance osseuse est réduite ou non modifiée par l'ingestion de fluor.
    Abstract: Zusammenfassung Die therapeutische Verwendung von Natriumfluorid ist für eine Anzahl von Knochenmangel-Krankheiten empfohlen worden. Die Empfehlungen basieren hauptsächlich auf den bekannten osteosklerotischen Wirkungen von Natriumfluorid; über dessen Effekt auf die Knochenstärke ist wenig bekannt. In dieser Arbeit wurde der Einfluß verschiedener Konzentrationen von Natriumfluorid auf die Knochenstärken von wachsenden Ratten mit hoher und niederer Calciumeinnahme untersucht. Die Verabreichung von Natriumfluorid erhöhte den Knochendurchmesser, was auf eine Stimulierung der Periostbildung hinwies, die Knochenstärke wurde jedoch durch Fluorideinnahme herabgesetzt oder nicht beeinflußt.
    Notes: Abstract The therapeutic use of sodium fluoride has been recommended in a variety of osteopenic bone diseases. The recommendations are based mainly on the known osteosclerotic effects of sodium fluoride and little information is available as to its effect on bone strength. The influence of various concentrations of sodium fluoride on bone strength in growing rats on high and low calcium diets was studied. The administration of sodium fluoride increased bone diameter, indicating stimulation of periosteal bone formation, but bone strength was reduced or not affected by fluoride ingestion.
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    URL: http://dx.doi.org/10.1007/BF02060302
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  • 24
    Electronic Resource
    Electronic Resource
    Special aspects of firing rates and thermosensitivity of preoptic neurons (1974)
    Springer
    Experimental brain research 21 (1974), S. 107-112 
    Details
    ISSN: 1432-1106
    Keywords: Preoptic units ; Thermosensitivity ; Mean firing rate ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Mean firing rates (per 10 sec) of preoptic neurons of the rat were recorded at normal temperature and after central warming and cooling. Thermosensitivity was determined according to the reactions of the mean firing rate. In compatibility with results from other animals we obtained the following percentages (52 neurons): 23% warm-sensitive, 13% cold-sensitive, 64% non-thermosensitive neurons. However, it turned out that a lot of interesting special effects are not taken into account, using exclusively the criterion “increasing/decreasing/constant mean firing rate”. Particularly we observed long lasting adaption processes and stationary non-linear effects as well as striking oscillations which were dependent on the thermal stimuli, while the mean interval value remained constant.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00234261
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  • 25
    Electronic Resource
    Electronic Resource
    The contribution of solvent drag to the intestinal absorption of the basic drugs amidopyrine and antipyrine from the jejunum of the rat (1974)
    Springer
    Naunyn-Schmiedeberg's archives of pharmacology 281 (1974), S. 175-196 
    Details
    ISSN: 1432-1912
    Keywords: Intestinal Absorption ; Basic Drugs ; Amidopyrine ; Antipyrine ; Solvent Drag ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary 1. Jejunal loops of anaesthetized rats were perfused with hypotonic, isotonic and hypertonic buffered solutions containing 14C-labelled amidopyrine and antipyrine at neutral and at acidic pH. The blood flow of the loop was maintained at an intermediate rate (0.7–1.0 ml min−1g−1). The water net flux was determined by means of polyethylene glycol as non-absorbable marker and amounted up to ±30 μl min−1g−1. 2. A positive water net flux (directed towards the blood) increased the appearance rate of amidopyrine and antipyrine by maximally 43.8 and 49.2%, a negative water net flux (directed towards the gut lumen) diminished it by 38.8 and 35.0%. 3. The experimental data were analysed by means of a kinetic model with the water net flux as independent variable and the epithelial permeability kF D , the serosal permeability k S F S and the sieving coefficient Φ=1−σ as absorption parameters. For antipyrine independent of the pH-value of the perfusion solution kF D was 0.123, and, for amidopyrine at pH 7 and pH 3 kF D was 0.231 and 0.091, respectively. k S F S was zero indicating that in this experimental arrangement the transfer of drug molecules to the serosal side was negligible. The sieving coefficient Φ amounted to 2.30 for amidopyrine and 2.15 for antipyrine at neutral pH. At acidic pH it amounted to 0.50 for amidopyrine and 1.44 for antipyrine. The hydraulic permeability of water was identical at neutral and acidic pH. 4. The high sieving coefficient for the two drugs at neutral pH is interpreted as indicating that water and lipophilic drug molecules interact within the lipid part of the cell membrane. At acidic pH the ionized drug molecules appear to permeate the cell membrane preferentially across other (presumably more hydrophilic) areas.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00503497
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  • 26
    Electronic Resource
    Electronic Resource
    The influence of blood flow on the phlorizine-insensitive and sensitive galactose absorption in rat jejunum (1974)
    Springer
    Naunyn-Schmiedeberg's archives of pharmacology 282 (1974), S. 195-212 
    Details
    ISSN: 1432-1912
    Keywords: Intestinal Blood Flow ; Intestinal Absorption ; d-Galactose ; Phlorizine ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary 1. Jejunal loops of anaesthetized rats were perfused with buffered solutions (pH 6.8) containing d-galactose in two concentrations: below (100 μM) and above (100 mM) blood sugar concentration. The blood flow rate, the appearance rate (in the intestinal venous blood) and the disappearance rate (from the intestinal lumen) were determined simultaneously, the accumulation in gut tissue at the end of the experiments. 2. At constant intermediate blood flow the appearance rate and disappearance rate decreased with time. A decrease of the blood flow rate from about 1.9 to about 0.3 ml min−1 g−1 diminished both the appearance and the disappearance rate. An increase of blood flow from about 0.3 to about 1.9 ml min−1 g−1 did not increase the absorption rates; even a slight decrease was observed. 3. In the presence of phlorizine (2 mM) the absorption rates and the tissue accumulation of galactose is reduced. At constant intermediate blood flow the appearance and disappearance rate increased with time. A decrease of the blood flow rate from about 1.8 to about 0.3 ml min−1 g−1 diminished the appearance rate. An increase of the blood flow rate from about 0.3 to about 1.8 ml min−1 g−1 caused an increase of the appearance rate in contrast to the experiments without phlorizine. Therefore, the different blood flow dependence of the intestinal absorption observed with actively transported substances can be attributed to their non-passive component.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00499034
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    Electronic Resource
    Fenfluramine-induced ultrastructural alterations in tissues of rats and guinea pigs (1974)
    Springer
    Naunyn-Schmiedeberg's archives of pharmacology 285 (1974), S. 175-184 
    Details
    ISSN: 1432-1912
    Keywords: Fenfluramine ; Rat ; Guinea Pig ; Phospholipidosis, drug-induced ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary After administration of the anorectic drug fenfluramine (40 mg/kg per day) to rats (s. c. or i. p.) and guinea pigs (p.o.) over periods of 2 to 11 weeks, electron microscopic examination was performed on lungs, liver, lymphatic tissues and peripheral blood of both species, and on adrenal glands and ovaries of rats. In rats, fenfluramine caused the following alterations (listed according to the duration of treatment): Formation of abnormal lamellated inclusions in lymphocytes and plasma cells of lymphatic tissues, appearance of “foam cells” in lung alveoli; formation of abnormal lamellated inclusions in corpus luteum, lymphocytes of peripheral blood, and in adrenal cortex. In guinea pigs, the same alterations were found in lymphocytes of spleen and peripheral blood, in hepatocytes and in lung. The present observations support the concept of a generalized phospholipidosis induced by amphiphilic compounds. The potency of fenfluramine to induce a lipidosis is, however, considerably less pronounced than that previously demonstrated for the anorectic drug chlorphentermine. This difference is suggested to be due mainly to the lower degree of amphiphilia of fenfluramine.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00501152
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  • 28
    Electronic Resource
    Electronic Resource
    The action of chlorpromazine on exploration in pairs of rats (1974)
    Springer
    Psychopharmacology 37 (1974), S. 249-254 
    Details
    ISSN: 1432-2072
    Keywords: Chlorpromazine ; Rat ; Social Interaction ; Exploration ; General Motor Activity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract When two animals were placed in a hole-board the number of headdips made by each rat was twice the number made when tested alone, but was the same for pairs of drugged (2 mg/kg chlorpromazine) and undrugged rats, and for pairs where only one rat was drugged. This contrasts with the reduction of headdipping produced by chlorpromazine in single animals. However, chlorpromazine still reduced general motor activity, regardless of whether the partner was drugged or not. The activity of the undrugged rats was affected by the partner's state and was lower when the partner was drugged.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00421538
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  • 29
    Electronic Resource
    Electronic Resource
    Effects of low and high doses of l-Dopa on the tetrabenazine or α-methyltyrosine-induced suppression of behaviour in a successive discrimination task (1974)
    Springer
    Psychopharmacology 39 (1974), S. 199-212 
    Details
    ISSN: 1432-2072
    Keywords: Rat ; Successive Discrimination ; Conditioned Avoidance Response ; Nerve Impulses ; α-Methyltyrosine ; Tetrabenazine ; l-Dopa
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The effect of the administration of l-Dopa, 10 or 100 mg/kg i.p., on the α-methyltyrosine methylester HCl (α-MT; 250+50 mg/kg i. p.) or tetrabenazine (TBZ; 2 mg/kg i.p.)-induced suppression of a successive discrimination-conditioned avoidance task has been studied. It was found that administration of the high l-Dopa dose resulted in a reversal of the α-MT- or TBZ-induced suppression of the avoidance behaviour, although the discrimination was lost. Restoration of the avoidance behaviour and the correct discrimination was obtained with the low l-Dopa dose after α-MT but not after TBZ. The difference in the behavioural response to l-Dopa after α-MT as compared to the response obtained after TBZ is discussed in terms of the availability of the catecholamines formed from l-Dopa for release by the nerve impulses. Biochemical determinations of brain noradrenaline and dopamine were made in parallel. Further, a simple and reliable method for the aquisition of a successive discrimination in the rat is described.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00421027
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  • 30
    Electronic Resource
    Electronic Resource
    Action of a new psychotropic drug (Sulpiride) on avoidance behavior in rats (1974)
    Springer
    Psychopharmacology 39 (1974), S. 309-314 
    Details
    ISSN: 1432-2072
    Keywords: Avoidance ; Drug ; Psychotropic ; Rat ; Sidman Schedule ; Sulpiride
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Sulpiride, a new psychotropic drug with neuroleptic activity, was administered to rats in a Sidman avoidance schedule. Doses ranging from 1 to 80 mg/kg resulted in the animal receiving fewer shocks. This effect was not correlated with an increase of responding and seemed unrelated to the suppressing action abruptly observed at high doses (i. e. 150 to 200 mg/kg). These results differ from those classicaly obtained with other neuroleptics.
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    URL: http://dx.doi.org/10.1007/BF00422970
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  • 31
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    Electronic Resource
    Behavioural and biochemical effects of 6-hydroxydopa in rats (1974)
    Springer
    Psychopharmacology 35 (1974), S. 341-352 
    Details
    ISSN: 1432-2072
    Keywords: 6-Hydroxydopa ; Rat ; Behaviour ; Biogenic Amines
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The influence of 6-hydroxydopa (6-HDP) injected into the lateral ventricles of rat brain on the behaviour of animals was examined. The level of noradrenaline (NA), 5-hydroxytryptamine (5-HT) and 5-hydroxyindoleacetic acid (5-HIAA) in discrete areas of the brain was also measured. 6-HDP was injected in a dose of 100 or 150 Μg into both lateral ventricles, or in a dose of 200 Μg into the right lateral ventricle. Immediately after 6-HDP injection, circling movements, convulsions, aggressive behaviour, and Stereotypic activity were seen. These components of behaviour were most obvious during the first hour after injection of 6-HDP. During one month after 6-HDP administration the behaviour of rats did not differ significantly from the behaviour of control animals, only very subtle differences in behaviour being seen. The dose of 150 Μg of 6-HDP caused aphagia and loss of body weigth during the first 5 days after treatment. 6-HDP also caused hypothermia. 20 min after administration of 200 Μg of 6-HDP a decrease of the NA level but no changes in the 5-HT level in the brain cortex were seen. The same changes were observed 1 month after 6-HDP treatment. The dose of 150 Μg of 6-HDP decreased the NA level in the brain cortex, cerebellum, mesencephalon and brain stem 5 days after treatment. 5-HT content was not changed and the 5-HIAA level was increased in the same brain areas. The dose of 100 Μg of 6-HDP, 2 weeks after the treatment decreased the content of NA in the brain cortex, cerebellum, hypothalamus and brain stem without changes in 5-HT content. 5-HIAA level was elevated only in the brain stem. It is concluded that 6-HDP is a long-acting potent depletor of NA in rat brain. There is a different sensitivity of brain areas to the depleting effect of 6-HDP. 6-HDP does not deplete the 5-HT content of discrete areas of brain but increases the level of 5-HIAA. 6-HDP causes distinct behavioural changes shortly after the treatment. It does not change the behaviour of rats between 1 and 30 days after its administration.
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    URL: http://dx.doi.org/10.1007/BF00429225
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  • 32
    Electronic Resource
    Electronic Resource
    Conditioned aversion in spatial paradigms following methamphetamine injection (1974)
    Springer
    Psychopharmacology 36 (1974), S. 323-335 
    Details
    ISSN: 1432-2072
    Keywords: Methamphetamine ; Rat ; Conditioned ; Aversion ; Space ; Saccharin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Three experiments were performed on Sprague-Dawley derived rats in which 3.0 mg/kg, i.p., injections of methamphetamine were paired with either, (1) a distinctive spatial enclosure, i.e., either a black or gray portion of a shuttle box with discriminably different flooring, (2) the enclosure plus 0.1% saccharin solution availability in the home cage, or (3) the enclosure plus 0.1% saccharin solution availability in that area. Following three drug pairings which alternated with saline injections paired with a different enclosure or enclosure plus H2O availability, a choice of either spatial (1), or spatial plus gustatory stimuli (2,3) which included the original CS's, were presented under non-drug, non-injection conditions. Control groups received saline injections each day under identical conditions. No aversion to the enclosed area, which had been paired with the drug was demonstrated under (1), gustatory (P=0.005) but not spatial aversion was in evidence in the second paradigm, and spatial (P=0.01) and gustatory (P〈0.01) aversion was exhibited in the last study. It was concluded that subsequent aversion to distinctive spatial enclosures following methamphetamine injections was not as strong an effect as similar aversions to gustatory stimuli. Furthermore, the effect only obtained to neutral and not to preferred spatial areas at the dose level tested and in the apparatus used in those studies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00422564
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  • 33
    Electronic Resource
    Electronic Resource
    In vivo studies on uricase activity in the rat (1974)
    Springer
    Pflügers Archiv 351 (1974), S. 323-330 
    Details
    ISSN: 1432-2013
    Keywords: Uricase ; Urate ; Allantoin ; Liver ; Kidney ; Microperfusion ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary 1. In vivo uricase activity was tested in rats by injection of 2-C14 urate and measurement of the total C14 activity and the fractional activities of allantoin, allantoic acid and urea in samples of blood and urine. In control animals, 5 min after the injection, 70% of the plasma tracer was already present in the form of allantoin. No allantoic acid and urea were produced. Intestinectomy had no measurable influence on uricase activity. On the other hand, hepatectomy or ligation of the hepatic artery combined with subtotal viscerectomy did abolish uricase activity almost completely. 2. Following microinjections into proximal tubules of Ringer solution containing 2-C14 urate, urine samples during early recovery mainly contained labelled urate, whereas in later samples the fraction of labelled allantoin increased. About 12 min after the microinjection the urine of both kidneys contained equal amounts of tracer mainly in the form of allantoin. 3. When segments of proximal tubules were perfused with an equilibrium solution containing tracer amounts of C 14 urate, no urate was metabolized during its passage through the proximal tubule. 4. C 14 urate was offered from the peritubular capillaries and samples of tubular fluid were analyzed, Again, all the tracer in the tubular fluid was in the form of urate, indicating that urate is not oxidized when it is transported across the tubular cell. It is concluded from these results that: 1. The rat kidney has no significant uricase activity. 2. Urate transport in the kidney is not influenced by this enzyme. 3. The degradation of urate to allantoin takes place at extrarenal sites, mainly in the liver.
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    URL: http://dx.doi.org/10.1007/BF00593318
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  • 34
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    Electronic Resource
    Cardiac hypertrophy in the first generation of rats native to simulated high altitude (1974)
    Springer
    Pflügers Archiv 350 (1974), S. 241-248 
    Details
    ISSN: 1432-2013
    Keywords: Cardiac Hypertrophy ; Diffusion Distance of Oxygen ; High Altitude ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In rats born and staying their whole life at a simulated altitude of 3500 m a severe cardiac hypertrophy develops which is most pronounced in the right ventricle. On histological sections muscle fiber and capillary densities were counted and the surfaces occupied by muscle fibers and capillaries in the slides were estimated. Mean muscle fiber diameter and half mean distance between capillaries (diffusion distance) were obtained in the heart ventricles and were compared with those of control animals. In both ventricles an increase of the mean muscle fiber diameter occurred (true hypertrophy), being more pronounced in the right ventricle. We cannot tell whether also hyperplasia of muscle fibers occurred or not. The diffusion distance in the left ventricle of rats exposed to hypoxia was about the same as in control animals but was shorter in the right ventricle. This indicates that the total number of capillaries is higher in both ventricles of rats born in the low pressure chamber (hyperplasia of the capillaries). The shortening of the diffusion distance in the right ventricle of rats born and living their whole life at simulated high altitude was on the average smaller than that of rats exposed to simulated high altitude later in life. The physiological importance of this observation is discussed.
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    URL: http://dx.doi.org/10.1007/BF00587803
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  • 35
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    Electronic Resource
    Analysis of periodic components of hypothalamic spike-trains after central thermal stimulation (1974)
    Springer
    Pflügers Archiv 351 (1974), S. 13-24 
    Details
    ISSN: 1432-2013
    Keywords: Hypothalamus ; Spike-Trains ; Correlogram/Periodogram ; Thermosensitivity ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Stationary spike-trains of preoptic neurons of the rat were submitted to a correlation- and spectral-analysis at normal temperature and after central warming and cooling. Correlograms and periodograms were computed which enable the elimination of stochastic signal components and the identification of the relevant periodic components. About one half of the analysed neurons showed periodic correlograms. The following three classes may be distinguished: Neurons which showed correlograms with periodicities at each applied thermal state, at only one or two thermal states and those with no recognizable periodicities at all. Relations between periodicity and the thermosensitivity of the neurons are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00603507
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  • 36
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    Demonstration by degeneration silver method of the ascending projection from the locus ceruleus (1974)
    Springer
    Experimental brain research 20 (1974), S. 181-192 
    Details
    ISSN: 1432-1106
    Keywords: Locus ceruleus ; Cerulo-cortical tract ; Ascending reticular fiber ; Rat ; Fink-Heimer method
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In order to demonstrate the ascending projection from the locus ceruleus by degeneration silver method, the nucleus was destroyed unilaterally in rats and the brains were stained by a modified Fink-Heimer method following various survival times. Additional experiments included the bilateral destruction of the locus ceruleus and the administration of 6-OH-DOPA. For the demonstration of degenerated terminals in the cerebral cortex it is essential to choose an optimal survival time of 4 or 5 days, keep the time of fixation below 1 week, and perform treatments with permanganate and with silver at 37°C. The trajectory and distribution of the cerulo-cortical tract as revealed by the silver degeneration methods is in agreement with the results of the histofluorescence method, with some minor differences. The ascending fibers run through Forel's tegmental fascicle, turn rostro-ventrally to the subthalamus, enter and cross the internal capsule, pass through the ventral part of the caudate-putamen and distribute to the entire cerebral cortex. The tract is mainly ipsilateral, though some fibers are crossing.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00234012
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  • 37
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    Electronic Resource
    Comparative study of the absorption, elimination and acute hepatotoxic action of ethanol in guinea pigs and rats (1974)
    Springer
    Archives of toxicology 32 (1974), S. 83-95 
    Details
    ISSN: 1432-0738
    Keywords: Alcohol, Ethyl ; Absorption ; Elimination ; Alcohol Oxidoreductases ; Hepatitis, Toxic ; Transaminases ; Glutamate Dehydrogenase ; Guinea Pig ; Rat ; Äthylalkohol ; Resorption ; Elimination ; Alkoholdehydrogenase ; Hepatotoxicität ; Transaminasen ; Glutamatdehydrogenase ; Meerschweinchen ; Ratte
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Die maximalen Blutalkoholkonzentrationen nach oraler Gabe von 6,4 g/kg Äthanol in Form einer 40%igen Lösung (v/v) betrugen bei nicht nüchternen Meerschweinchen 6,8±0,3 mg/ml, bei nicht nüchternen Ratten dagegen nur 2,1±0,2 mg/ml. An nüchternen Tieren führte eine orale Alkoholbelastung von 4,8 g/kg zu maximalen Blutalkoholspiegeln von 6,3±0,2 mg/ml bei den Meerschweinchen und 3,7±0,3 mg/ml bei den Ratten. Intravenös injizierter Alkohol (1 g/kg) wurde demgegenüber von beiden Species mit der gleichen Geschwindigkeit eliminiert (275 mg/kg · Std), und die auf das Körpergewicht bezogene ADH-Aktivität der Leber war bei den Meerschweinchen um 20% größer als bei den Ratten. Die Resorption von Äthanol erfolgt somit bei Ratten erheblich langsamer als bei Meerschweinchen. Dies dürfte darauf beruhen, daß konzentrierte Alkohollösungen die Magenentleerung der Ratte stark verzögern. Niedriger konzentrierte Alkohollösungen werden von der Ratte schneller resorbiert; jedoch waren auch nach Gabe von 10%igem Alkohol die maximalen Blutalkoholspiegel bei Ratten noch um 36% niedriger als bei Meerschweinchen. Bei den Meerschweinchen waren nach oraler Gabe von 4,8 bzw. 6,4 g/kg Äthanol die Serumaktivitäten der GOT, GPT und GLDH erhöht; ein Anstieg der SGOT trat schon nach 1,6 g/kg Äthanol auf. Demgegenüber kam es bei Ratten nach 6,4 g pro kg Äthanol p.o. nur zu geringfügigen Aktivitätserhöhungen der Serumtransaminasen, während die Serumaktivität der GLDH unverändert blieb. Vacuolige Degeneration war das morphologische Substrat der durch Äthanol bei Meerschweinchen und Ratten ausgelösten Leberschädigung. Während diese Veränderung bei Meerschweinchen bereits nach einer Äthanoldosis von 1,6 g/kg auftrat, wurde sie bei Ratten erst nach 6,4 g/kg beobachtet. Schlußfolgerung: Das Meerschweinchen ist für Untersuchungen zur Toxicität von Äthanol besser geeignet als die Ratte.
    Notes: Abstract In fed guinea pigs, an oral dose of 6.4 g/kg of ethanol given as a 40% solution (v/v) produced a maximal blood alcohol level of 6.8±0.3 mg/ml, whereas in fed rats, blood alcohol levels after the same dose did not exceed 2.1±0.2 mg/ml. Maximal blood alcohol levels in fasted animals after an oral load of 4.8 g/kg of ethanol were 6.3±0.2 mg/ml in guinea pigs and 3.7±0.3 mg/ml for rats. However, i.v. injected ethanol (1 g/kg) was eliminated at the same rate in both species (275 mg per kg · h), and ADH activity of the liver related to body weight was by 20% greater in guinea pigs than in rats. Therefore, absorption of ethanol occurs at a much slower rate in rats than in guinea pigs. This is possibly due to the fact that high ethanol concentrations strongly delay emptying of the rat stomach. Lowering the ethanol concentration accelerates absorption rate in the rat. However, even after gavage of a 10% solution peak levels of blood alcohol were still lower by 36% in rats than in guinea pigs. In guinea pigs, increased serum activities of GOT, GPT, and GLDH occurred after an oral dose of 4.8 g/kg or 6.4 g/kg of ethanol, respectively. SGOT already increased after 1.6 g/kg of ethanol p.o. After 6.4 g/kg of ethanol given to rats serum transaminase levels increased only slightly, and GLDH activity not at all. Vacuolar degeneration was the morphological substrate of ethanol-induced liver damage in guinea pigs and rats. In guinea-pigs, it occurred already after 1.6 g/kg of ethanol, whereas in rats only after 6.4 g/kg. In conclusion, the guinea pig seems to be better suited for research on alcohol toxicity than the rat.
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    URL: http://dx.doi.org/10.1007/BF00316229
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  • 38
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    Resistance to extinction of an avoidance response in rats following the administration of chlordiazepoxide (Librium) or diazepam (Valium) (1974)
    Springer
    Psychopharmacology 38 (1974), S. 231-238 
    Details
    ISSN: 1432-2072
    Keywords: Rat ; Chlordiazepoxide ; Diazepam ; Extinction ; Avoidance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract In a one-way avoidance task with rats, injections of Librium (chlordiazepoxide) following avoidance acquisition resulted in prolonged resistance-to-extinction of the avoidance response. This effect occurred regardless of whether the rats had had prior experience with Librium or whether they were naive with respect to the drug. The same results were found with the same task when low doses of Valium were used. However, at a higher dosage an “extreme reaction” of either no responding or a high number of responses to extinction occurred in the naive animals. The Librium and Valium effects were compared to similar effects obtained using ethanol and hashish resin. These results indicate that the novelty hypothesis as originally stated by Amit and Baum cannot be supported because experience with the drugs prior to avoidance training did not attenuate the drug effect on avoidance.
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    URL: http://dx.doi.org/10.1007/BF00421375
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  • 39
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    Effect of LSD-25 and 1-methyl-d-lysergic acid butanolamide on rat brain and platelet serotonin levels (1974)
    Springer
    Psychopharmacology 36 (1974), S. 117-122 
    Details
    ISSN: 1432-2072
    Keywords: LSD-25 ; UML ; 5-HT ; Brain ; Platelets ; Behaviour ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Factorial analysis of rat cerebral 5-HT values observed following intraperitoneal LSD-25 and UML (1-methyl-d-lysergic acid butanolamide) showed that the highly significant decrease induced by both drugs was independent of dose and that the effect of both drugs was the same. Increased platelet 5-HT values were also obtained with each drug. These findings suggest that the psychotomimetic effect of LSD-25 is not due to changes in cerebral 5-HT, since a relationship can probably be postulated between such an effect in man and the effect of LSD-25 on conditioned behaviour in the rat. It is also felt that the fact that these drugs decrease and increase cerebral and platelet values respectively may be of assistance in the interpretation of data relating to platelet 5-HT patterns in man under psychiatric conditions and during treatment with psychotropic drugs.
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    URL: http://dx.doi.org/10.1007/BF00421783
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  • 40
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    Effect of acute and chronic ethanol administration on serotonin turnover in rat brain (1974)
    Springer
    Psychopharmacology 37 (1974), S. 91-100 
    Details
    ISSN: 1432-2072
    Keywords: Ethanol ; Brain Serotonin Turnover ; Tolerance ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract In rats treated acutely and chronically with ethanol, brain serotonin (5-HT) turnover was determined by measuring in the same animals the rate of accumulation of 5-HT and the rate of decline of 5-hydroxyindoleacetic acid (5-HIAA) in the brain after administration of the MAO inhibitor pargyline-HCl. In the acute study, 5-HT turnover was not altered after a single 4 g/kg oral dose of ethanol. In two separate studies, it was shown that chronic administration of ethanol in the form of a liquid diet for 32 days also did not alter brain 5-HT turnover, even when the rats had developed a significant degree of tolerance to the motor-impairing effects of ethanol. These results suggest that tolerance to ethanol is unrelated to any change in brain 5-HT turnover.
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    URL: http://dx.doi.org/10.1007/BF00437416
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  • 41
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    Electronic Resource
    Urate handling by the rat kidney (1974)
    Springer
    Pflügers Archiv 352 (1974), S. 115-120 
    Details
    ISSN: 1432-2013
    Keywords: Urate ; Reabsorption ; Loop of Henle ; Micropuncture ; Microperfusion ; Microinjection ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Reabsorption rates for urate in the loops of Henle were measured in superficial nephrons in the rat 1. under conditions of free flow, 2. using microperfusion and 3. by a microinjection technique. 1. Under conditions of free flow distally measured TF/PUA/TF/PIn-values varied between 0.51 and 0.38 in antidiuretic rats, depending on TF/PIn (UA = both uric acid and urate, In = inulin, TF/P = concentration in tubular fluid to plasma concentration). The corresponding values in samples from end-proximal tubules were 1.06 and in urine 0.19 (U/PUA/U/PIn). 2. In microperfusion experiments of Henle loops early distal recoveries of 2-C14 urate varied between 57 and 86%, depending on the flow rates (10–40 nl/min). 3. In microinjection experiments C14 recovery in urine was about 85% when tracer solution was microinjected into endproximal tubules. From these results we conclude: 1. The main site of urate reabsorption is located in the loops of Henle. 2. This reabsorption is highly dependent on flow rates. Increase of flow rate through Henle's loop decreases urate reabsorption.
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    URL: http://dx.doi.org/10.1007/BF00587510
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  • 42
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    Urate interaction with plasma proteins and erythrocytes (1974)
    Springer
    Pflügers Archiv 352 (1974), S. 121-133 
    Details
    ISSN: 1432-2013
    Keywords: Urate ; Protein Interaction ; Uptake by Erythrocytes ; Renal Reabsorption ; Man ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Interaction of urate with human and rat plasma was studied by a dialysis technique at different temperatures. At 4° C a certain fraction of urate is bound to proteins. However, this fraction diminishes with increasing temperature and at 37° C only some 7–8% (in man) and 2% (in rat) interact with proteins. The finding that urate is almost completely filtered in the glomerulus is discussed. In body areas exposed to low temperatures protein binding of urate may be of importance. Urate uptake by erythrocytes is characterized by two components: a fast component equilibrating almost immediately at about 7% in man and 17% in rat and a slow component reaching equilibrium after 60 min, at 28% and 36%, respectively. The process is described by a mathematical formula. Lowering of the temperature mainly diminishes uptake by the slow component withQ 10-values ranging between 1.5 and 4.0. In the observed range of concentrations the uptake process does not saturate. The results at lower pH-values suggest that it is unionized uric acid which is transported by the slow component. Application of the data to kidney medulla supports the hypothesis that erythrocytes and, probably, to a lesser extent plasma proteins serve as vehicles for urate reabsorption in the countercurrent system. Such a temporary interaction enables uric acid to escape recirculation and to leave the kidney medulla.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00587511
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    The effects of scopolamine and physostigmine on fixed-interval behaviour in the rat (1974)
    Springer
    Psychopharmacology 39 (1974), S. 237-244 
    Details
    ISSN: 1432-2072
    Keywords: Antagonism ; Fixed Interval ; Scopolamine ; Physostigmine ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The purpose of this research was to determine if 2 drugs, scopolamine and physostigmine which have opposite effects on cholinergic transmission also produce opposite effects on fixed-interval (FI) performance. Scopolamine and a wide range of doses of physostigmine were administered singly and concurrently to a number of rats responding on a FI 2 min schedule. Scopolamine produced a disruption in FI curvature which could be antagonized by physostigmine, but physostigmine by itself produced no consistent change.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00421031
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  • 44
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    Electronic Resource
    The role of sex and novelty in determining the social response to lithium chloride (1974)
    Springer
    Psychopharmacology 40 (1974), S. 91-99 
    Details
    ISSN: 1432-2072
    Keywords: Lithium ; Social Behaviour ; Sex ; Novelty ; Photographs ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Effects of lithium chloride on the social behaviour of male and female rats were measured in unfamiliar and familiar test situations using a photographic technique. Lithium-treated females showed an increase in body contact and a decrease in social distances in comparison with saline-treated controls in both test conditions. Males administered lithium showed increased corner occupancies in the novel test setting while in the familiar test they showed decreased body contact and increased average group social distance in comparison with the saline condition. Further tests in an exploration box confirmed that lithium decreased the general environmental responsiveness of male rats. The results are discussed with reference to the anti-aggressive effect of lithium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00429450
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  • 45
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    In vitro analysis of the cellular localization of luteinizing hormone releasing factor (LRF) in the basal hypothalamus of the rat (1974)
    Springer
    Cell & tissue research 149 (1974), S. 371-378 
    Details
    ISSN: 1432-0878
    Keywords: LRF ; Hypothalamic localization ; Rat ; Ependyma ; Glia ; Light and electron microscopic Autoradiography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Light and electron microscopic autoradiographic analysis of the medial basal hypothalamus of rat brains incubated for 30 and 60 minutes in tritiated luteinizing releasing factor (3H-LRF) revealed selective uptake in the form of silver grains over specialized ependyma (tanycytes) and perivascular glial cells in the palisade-contact zone of the median eminence. The functional capacity of tanycytes and glia are discussed in light of these data.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00226771
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  • 46
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    Electronic Resource
    Failure to demonstrate serotonergic neurons in the myenteric plexus of the rat (1974)
    Springer
    Cell & tissue research 150 (1974), S. 493-496 
    Details
    ISSN: 1432-0878
    Keywords: Serotonin ; Neurotransmitter ; Myenteric plexus ; Rat ; Histofluorescence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Several recent studies suggested that serotonergic neuron-like elements are present in the guinea pig ileum. The present paper reports an extensive study of the digestive tract of the rat with the use of a histofluorescence technique. Administration of the serotonin precursor, tryptophan, associated with a monoamine oxidase inhibitor, did not allow histochemical demonstration of rapidly fading, yellow fluorescent, 6-hydroxydopamine-resistant neurons; conversely such neurons were readily detected in the brain. It is concluded that serotonergic neuron-like elements cannot be detected histochemically in the rat myenteric plexus area after chemical sympathectomy.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00225972
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  • 47
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    Electronic Resource
    Studies on the juxtaglomerular apparatus (1974)
    Springer
    Cell & tissue research 151 (1974), S. 457-469 
    Details
    ISSN: 1432-0878
    Keywords: Juxtaglomerular apparatus ; Rat ; Adrenalectomy ; Renin and secretory granules ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Die Reaktion des juxtaglomerulären Apparates auf Adrenalektomie wurde untersucht. Im Stadium der Nebenniereninsuffizienz, sechs Tage nach der Operation, wurde quantitativ morphometrisch eine Abnahme der Volumendichte der Sekretgranula (von 32% auf 16%) festgestellt. Golgi-Apparat und Ergastoplasma proliferieren stark, wobei insbesondere die erweiterten Cisternen des Ergastoplasmas auffallen.
    Notes: Summary The response of the Juxtaglomerular apparatus to bilateral adrenalectomy was studied in rats. After the sixth postoperative day, symptoms of severe adrenal insufficiency had developed. In the Juxtaglomerular cells, the volume density of the secretory granules decreased from 32% to 16%. The Golgi apparatus and rough endoplasmic reticulum underwent considerable proliferation, the latter showing strikingly enlarged cisternae.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222991
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  • 48
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    Electronic Resource
    Sex differences in adrenocortical structure and function (1974)
    Springer
    Cell & tissue research 151 (1974), S. 525-536 
    Details
    ISSN: 1432-0878
    Keywords: Adrenal cortex ; Rat ; Sex differences ; Gonadal hormones ; Karyometry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Adrenal glands of adult female rats are heavier than the glands of corresponding male rats. Postpubertal orchiectomy increases the adrenal weight, an effect restored by testosterone replacement. Under the same conditions ovariectomy of 8 weeks duration does not change the adrenal weight while estradiol replacement enhances the relative adrenal weight. Karyometric studies have shown that nuclei in the female zona fasciculata cells are larger (app. 18%) than those of the male. Similar but only slight differences (2%) were observed in the zona reticularis. Orchiectomy results in enlargement of cell nuclei within all zones of the adrenal cortex; testosterone replacement has the opposite effect. Ovariectomy of 8 weeks duration slightly enhances the volume of nuclei of the zona glomerulosa cells, has no effect on the nuclei of the zona fasciculata and reduces the volume of nuclei in the zona reticularis. Estradiol replacement reduces the volume of nuclei of the zona glomerulosa cells but increases nuclear volume in the zona fasciculata and in the zona reticularis. Thus testosterone has an inhibitory effect on the adrenal cortex of the rat while the physiologic effect of estradiol on the morphology of this gland, particularly on the zona fasciculata cells is rather dubious. The author wishes to thank Mrs. B. Westerska and Miss K. Siejak for excellent technical assistance.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222997
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  • 49
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    Electronic Resource
    Sex differences in adrenocortical structure and function (1974)
    Springer
    Cell & tissue research 151 (1974), S. 537-547 
    Details
    ISSN: 1432-0878
    Keywords: Adrenal cortex ; Rat ; Sex differences ; Gonadal hormone ; Stereologic studies
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Adult female rats have heavier adrenals than males. Orchiectomy increases the adrenal weight, an effect reversed by testosterone. Neither ovariectomy nor estradiol replacement has an effect on absolute adrenal weight. The stereologic analysis of the volume fractions of particular adrenocortical zones have shown that the volume fraction of the zona glomerulosa in females is smaller as compared with males. On the contrary, the volume fractions of the fasciculata and reticularis zones are higher in female than in male rats. In both sexes gonadectomy and gonadal hormone replacement has an effect on the volume fractions of particular adrenocortical zones, these changes being more pronounced due to orchiectomy or testosterone replacement. The adrenal cortex of all experimental groups contains a similar quantity of parenchymal cells. The cytoplasm volume of a single adrenocortical cell is higher in all zones of the female adrenals as compared with the male. Orchiectomy increases the volume of the cytoplasm in fasciculata and reticularis cells. This effect was reversed by testosterone. Neither ovariectomy nor estradiol replacement has an effect on the volume of the cytoplasm in the fasciculata and reticularis cells. These studies have shown that sex differences with regards to rat adrenal size and weight depend upon the “physiologic atrophy” of the male adrenal cortex, while neither ovariectomy (8 weeks after operation) nor estradiol replacement has a conspicuous effect on the morphologic changes of this gland.
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    URL: http://dx.doi.org/10.1007/BF00222998
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  • 50
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    Ultrastructural investigations of the palisade-shaped nerve fiber terminals of the normal hairs of rat's snout (1974)
    Springer
    Cell & tissue research 147 (1974), S. 259-269 
    Details
    ISSN: 1432-0878
    Keywords: Nerve endings ; Hair ; Rat ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Palisade-shaped nerve endings of the small normal hairs of the rat snout were examined with the electron microscope (fixation by perfusion). The terminals are located inside the ‘glassy membrane’ in the area of the neck of the hair root. The 10–20 radially arranged terminal axons are in direct contact with the basement membrane of the epithelium of the external root sheath. The axons are surrounded on all sides by leaf-shaped processes of the Schwann cells. The surfaces of these cell processes are marked by numerous vesicle-like invaginations (approx. 1000 Å dia.). Transverse sections from several areas of the palisadeshaped nerve endings are compared with longitudinal sections. In the upper area ‘empty’ vesicles (approx. 500–600 Å in diameter) occur, along with electron-dense vesicles (approx. 800–1100 Å in diameter); in the middle area, the axons are distended and contain accumulations of mitochondria.
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    URL: http://dx.doi.org/10.1007/BF00582800
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    The parietal layer of bowman's capsule in experimental hypertension (1974)
    Springer
    Cell & tissue research 147 (1974), S. 183-190 
    Details
    ISSN: 1432-0878
    Keywords: Kidney ; Rat ; Bowman's capsule ; Experimental hypertension
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The parietal layer of Bowman's capsule was examined in the rat in control animals and in animals made hypertensive by treatment with desoxycorticosterone acetatesaline and by clipping of the renal artery. At an early stage of elevation of blood pressure and, occasionally, prior to elevation of blood pressure, the epithelial cells of this layer showed basal cytoplasmic protrusion and reduplication accompanied by an increase in the thickness of the basement membrane. These changes preceded evidence of glomerular or renal arteriolar pathology. They presumably reflect either a response of this layer to increased blood pressure and modified glomerular function, or a direct effect of the factors that also induces blood pressure elevation. In either case, they imply a greater functional significance for the parietal layer than has usually been attributed to it.
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    URL: http://dx.doi.org/10.1007/BF00582793
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  • 52
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    The ultrastructure of the accessory sex organs of the male rat (1974)
    Springer
    Cell & tissue research 148 (1974), S. 57-67 
    Details
    ISSN: 1432-0878
    Keywords: Prostate ; Rat ; Cyproterone ; Alterations ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The dorsal lobe and the coagulating gland of the rat prostate were studied after administration of the anti-androgenic compound Cyp A for varying periods (7–20 days). There was a general involution of the organs with macroscopic atrophy and reduced amount of secretory material. Ultrastructurally the cells were pale, contained less organelles as compared with the controls, loss of cytoplasm and reduction both of cell height and width. The major changes also included reduction of the Golgi area, the rough endoplasmic reticulum and the number of free ribosomes. Within the dorsal lobe prominent nuclear changes were found, comprising different types of intranuclear inclusions of membranous configurations. The alterations observed in the present study are similar to the changes which were found in castrated animals and are consistent with atrophy. The results are discussed in relation to the available biochemical and autoradiographic data on the different lobes.
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    URL: http://dx.doi.org/10.1007/BF00224318
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  • 53
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    Electronic Resource
    Ultrastructure of mucosal mast cells in normal and compound 48/80-treated rats (1974)
    Springer
    Cell & tissue research 150 (1974), S. 95-105 
    Details
    ISSN: 1432-0878
    Keywords: Mucosal mast cells ; Rat ; Compound 48/80 ; Degranulation, Release ; Light microscopy ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Mast cells in the tongue and lamina propria of the duodenal mucosa, representing two cell types with different morphological, histochemical and functional properties have been studied under the electron microscope. Both cell types were found to contain similar moderately electron dense and homogeneous granules surrounded by a tight membrane constituting the basic ultrastructural characteristics of mast cells. The mucosal mast cells, however, contained fewer granules per cell and the individual granules often exhibited considerable variation in size. In addition the mucosal mast cells, unlike those of the tongue, peritoneum and skin, had a smooth plasma membrane lacking microvilli and possessed irregularly shaped or lobulated nuclei. In the tongue mast cells, administration of Compound 48/80 caused the formation of vacuoles around the granules, expulsion of granules from the cells and disintegration of the granular matrix, but no changes that could be attributed to 48/80 could be found in the mucosal mast cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00220383
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  • 54
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    Kinetics of gametogenesis (1974)
    Springer
    Cell & tissue research 154 (1974), S. 443-470 
    Details
    ISSN: 1432-0878
    Keywords: Oocytes and T-prospermatogonia ; Rat ; Quantitative analysis ; Autoradiography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Bei der Ratte findet die sexuelle Differenzierung der Gonade zwischen dem 14. und 15. Tag post conceptionem (p.c.) statt. Während dieser Zeit teilen sich die Oogonien und deren Parallelpopulation—die M-prospermatogonien (multiplying prospermatogonia) oder I-Gonocyten—sehr häufig. Um den 17. Tag p.c. tritt die letzte Generation der Oogonien bzw. der M-prospermatogonien in die Mitose. Die Mitosen bilden häufig “synchronisierte Gruppen”. Die postmitotischen Kerne ihrer Tochterzellen—der Oocyten und der T 1 prospermatogonien (primary transitional prospermatogonia) oder II-Gonocyten—sind klein. Ihr Chromatin ist in Form gröberer Schollen der Kernmembran angelagert. Auf diese Weise kommt das typische “krustenförmige” Aussehen bzw. die Ähnlichkeit mit den Prophasen der Oogonien und M-prospermatogonien zustande. Die Oocyten durchlaufen zunächst die G1-phase (etwa 10 Std Dauer) und treten dann—am Ende des Präleptotänstadiums—in die S-phase. Dann passieren sie die verschiedenen Stadien der meiotischen Prophase und treten vom 3. Tag post partum (p.p.) ab in das Dictyotänstadium. Die T1-prospermatogonien hingegen befinden sich etwa 10 Tage lang in der G1-phase, ohne wesentliche morphologische Veränderungen aufzuweisen. Vom 4. Tag p.p. an durchlaufen sie die S-Phase. Die S-Phasen-Dauer (D-S) beider Zellarten beträgt 11.5 Std und wurde durch Doppelmarkierung mit 14C- und 3H-Thymidin bestimmt. Als am besten geeignete Termine für die Bestimmung der D-S erwiesen sich bei den Oocyten der 18. Tag p.c. und bei den T1-prospermatogonien der 5. Tag p.p. Zu diesen beiden Zeitpunkten war die Anzahl der in die S-phase ein-und austretenden Oocyten bzw. T1-prospermatogonien gleich, die Zellen in S-phase befanden sich im “steady state”. Das Kernvolumen der Oogonien und M-prospermatogonien ist etwa doppelt so groß wie das der postmitotischen Oocyten und T1-prospermatogonien. Bis zum 5. Tage p.p. nimmt das Kernvolumen der Oocyten und T1-prospermatogonien um etwa das Fünffache zu. Der Degenerationsindex der Oocyten liegt wesentlich höher als der der T1-prospermatogonien; er ist postnatal besonders hoch. Aus den T1-prospermatogonien gehen am 4. und 5. Tage p.p. durch Teilung die T 2-prospermatogonien (secondary transitional prospermatogonia) hervor. Die Kerne dieses Zelltyps sind etwas kleiner also die der T1-prospermatogonien. Die T2-prospermatogonien treten am 6. Tage p.p. in die Mitose; es entstehen die ersten A-spermatogonien.
    Notes: Summary In the rat (Wistar-WU) sexual differentiation of the gonads occurs between days 14 and 15 post conception (p.c.). At this time the oogonia and their parallel population — the M-prospermatogonia (I-gonocytes)—divide rapidly. On about day 17 p.c., the last generation of oogonia and M-prospermatogonia, frequently arranged in synchronized clusters, enters mitosis. The postmitotic nuclei of their daughter cells—oocytes and T 1-prospermatogonia (II-gonocytes)—are small; coarse flakes of chromatin are associated with the nuclear membrane causing the typical “crustlike” appearance and the similarity with the prophases of oogonia and M-prospermatogonia. After the oocytes have passed a G1-phase of approximately 10 hr, they enter the S-phase at the end of the preleptotene stage. Then they pass the different stages of the meiotic prophase until they enter the dictyate stage from 3 day post partum (p.p.) onwards. The T1-prospermatogonia, on the other hand, spend a long G1-phase of about 10 days without any conspicuous morphological change before entering the S-phase from day 4 p.p. onwards. The duration of the S-pbase (D-S) of both cell types—oocytes and T1-prospermatogonia—as determined by the double labeling method with 14C- and 3H-thymidine is found to be 11.5 hr. The most favourable time for determining the D-S was day 18 p.c. for the oocytes and day 5 p.p. for the T1-prospermatogonia. On these two days the balance was reached between the cells entering and leaving the S-phase. The nuclear volumes of the postmitotic oocytes and T1-prospermatogonia are approximately half the size of those of their precursors. Until day 5 p.p. the nuclear volumes of the oocytes and T1-prospermatogonia increase about fivefold. The degeneration index of the oocytes is considerably higher than that of the T1-prospermatogonia; postnatally it is especially high. T 2 prospermatogonia arise by mitosis of the T1-prospermatogonia on day 4 and 5 p.p. The nuclei of this cell type are smaller than those of T1-prospermatogonia. T2-prospermatogonia enter mitosis on day 6 p.p and give rise to A-spermatogonia.
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    URL: http://dx.doi.org/10.1007/BF00219667
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    Development of serotonin-containing cells and the sympathetic innervation of the habenular region in the rat brain (1974)
    Springer
    Cell & tissue research 155 (1974), S. 231-243 
    Details
    ISSN: 1432-0878
    Keywords: Pinealocyte-resembling cells ; Habenular region ; Sympathetic nerve fibres ; Ontogenetic development ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The cells of the pineal gland, the pineal stalk, and the lamina intercalaris contain 5-HT and are innervated by sympathetic nerve fibres. These peripheral nerve fibres continue rostrally from the lamina intercalaris and run into the central nervous tissue of stria medullaris and the habenular nuclei. Pharmacological treatment to increase the cellular 5-HT content revealed that the sympathetic fibres are in close relation to yellow fluorescent cells embedded in the brain tissue. These yellow fluorescent cells develop very late in the ontogenetic development (three weeks or more postnatally) and are preceded by ingrowth of sympathetic fibres into the brain tissue. The results support the hypothesis that the cells found in the habenular region are of pinealocyte rather than neuronal nature, but it is possible that they differ in certain aspects from the cells of the pineal gland proper.
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    URL: http://dx.doi.org/10.1007/BF00221357
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  • 56
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    Electronic Resource
    Somatic spines in the supraoptic nucleus of the rat hypothalamus (1974)
    Springer
    Cell & tissue research 148 (1974), S. 203-211 
    Details
    ISSN: 1432-0878
    Keywords: Hypothalamus ; Rat ; Supraoptic nucleus ; Ultrastructure ; Somatic spines
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Electron microscopic studies of ultrathin serial sections of the perikarya and adjacent neuropil of neurons of the supraoptic nucleus (SON) of the adult male rat revealed varying forms of two types of somatic spines. One type forms synapses with axons passing the cell, the other, without synapses, appears to serve as a buttress or clasp for adjacent neuronal and glial processes. The synapse-bearing spines lack the usual spine apparatus but contain the flocculent substance often seen in spines. The other spines do not exhibit either of these structures. These somatic spines were also seen in Golgi impregnated preparations but the types could not be distinguished. Certain axons synapse either on a somatic spine of the perikarya or penetrate the glial sheath of the neuron and synapse, usually repeatedly, on the soma in an en passant manner.
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    URL: http://dx.doi.org/10.1007/BF00224582
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  • 57
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    Ultrastructure of rat pineal gland after preparation by Freeze-etching technique (1974)
    Springer
    Cell & tissue research 148 (1974), S. 371-379 
    Details
    ISSN: 1432-0878
    Keywords: Pineal gland ; Rat ; Electric coupling areas ; Freeze-etching
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The pineal glands of Wistar rats were fixed in glutaraldehyde and prepared by the freeze-etching technique. With regard to the form and internal structure of pineal cells this technique has revealed nothing essentially new. Synaptic ribbons are very rarely found. A regular diagonal arrangement of membrane attached particles between the Schwann cell and the axon of an autonomic nerve terminal was observed. Three types of contact areas between pineal cells are described. In the first two types, which occur more frequently, the diameter of the area varied from 1000 to 2500 Å, with irregularly aggregated membrane associated particles. The third type, larger but less frequently found, has a surface area of 0.4 μm2 and much more regularly distributed membrane associated particles (ø 88 Å). These contact areas can be considered to be electric coupling areas between pineal cells.
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    URL: http://dx.doi.org/10.1007/BF00224264
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  • 58
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    Electronic Resource
    Indoleamine-containing nerve terminals in the rat median eminence (1974)
    Springer
    Cell & tissue research 147 (1974), S. 285-292 
    Details
    ISSN: 1432-0878
    Keywords: Median eminence ; Rat ; Serotonin terminals ; 5,6- and 5,7-Dihydroxytryptamine ; Chemical degeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Electron microscopical evidence for the existence of an important serotonergic input to the rat median eminence is presented. This evidence is based on the demonstration of degenerating nerve terminals in the external layer of the rat median eminence following the application of 5,6-dihydroxytryptamine or 5,7-dihydroxytryptamine, drugs known to exert more or less selective toxic effects on central serotonin neurons. The finding of a serotonergic innervation of the median eminence in mammals has important implications for 5-hydroxytryptamine as a modulator of synthesis and/or release of hypothalamic, hypophysiotropic hormones.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00582802
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  • 59
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    Electronic Resource
    On the ultrastructure of the rat anococcygeus muscle (1974)
    Springer
    Cell & tissue research 149 (1974), S. 91-104 
    Details
    ISSN: 1432-0878
    Keywords: Anococcygeal muscle ; Smooth muscle ; Rat ; Innervation, adrenergic
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The rat anococcygeus muscle, which is known from previous functional and histochemical investigations to be a smooth muscle with a dense adrenergic innervation, was studied by electron microscopic methods. The muscle was found to be organized in small bundles of 2 to 8 muscle cells, which were interconnected by desmosome-like junctions and by gap junctions. Small Schwann cell-axon complexes with 1 to 5 axons appeared to represent the functional terminal nerve pathway. Such axon complexes were located at the periphery of the small smooth muscle bundles, they never penetrated into them. A ratio of approximately 6 small axon complexes per 100 muscle cells was found. After glutaraldehyde fixation axon varicosities contained mainly small empty vesicles. After treatment with 5-hydroxydopamine and permanganate fixation mainly small vesicles with a dense core were found. The neuromuscular distances ranged from 550 to 5000 Å, with a mean of about 2600 Å. True close neuromuscular contacts at a distance below 500 Å with a fusing of basal laminae were absent. The present observations provide a morphological base for the findings of previous electrophysiological and pharmacological studies on the anococcygeal muscle.
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    URL: http://dx.doi.org/10.1007/BF00209052
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  • 60
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    Ultramorphometric studies regarding the effect of sex-hormones on the adrenal cortex of the rat (1974)
    Springer
    Cell & tissue research 149 (1974), S. 113-120 
    Details
    ISSN: 1432-0878
    Keywords: Adrenal cortex ; Rat ; Sex-hormones ; Ultramorphometry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Ultramorphometrisch wurde nach dem Verfahren von Rohr et al. (1971) die äußere Zona fasciculata von Sprague-Dawley-Ratten bei 5 Gruppen zu je 4 Tieren verglichen: Männchen, Weibchen im Diöstrus, Weibchen im Östrus, männliche und weibliche Kastraten (Kastration am 4. Lebenstag). Die Nebennierenrindenzellen der äu\eren Fasciculata sind bei Weibchen gegenüber denen normaler Männchen hypertroph: Weibchen im Diöstrus zeigen gegenüber Männchen größere Einzelzellen mit vermehrtem glattem endoplasmatischem Reticulum, erhöhten Einzel- und Gesamtvolumina der Mitochondrien sowie einem höheren Lipoidgehalt bei stärkerer Dispersion der Liposomen. Dieselben funktionsspezifischen Strukturen nehmen bei den Weibchen im Östrus noch weiter zu. Die Kastration der Weibchen am 4. Lebenstag führt zu einer Reduktion der funktionsspezifischen morphometrischen Parameter unter die Werte der Diöstrus-Tiere. Männliche Kastraten gleichen die Werte ihrer funktionsspezifischen Strukturen weitgehend denen der Weibchen im Diöstrus an. Nach Kastration am 4. Lebenstag zeigen weibliche Tiere somit etwas niedrigere morphonietrische Parameter in der äußeren Zona fasciculata als die männlichen Kastraten.
    Notes: Summary With the method of Rohr et al. (1971) the outer zona fasciculata of Sprague-Dawley-rats was compared ultramorphometrically in 5 groups of 4 animals each: males, females during diestrus, females during estrus, male and female castrates (castration on 4th day of life). Adrenal cortical cells of the outer fasciculata in females are hypertrophic in comparison with those of normal males: females during diestrus show in comparison with males larger individual cells with increased SER, increased volume of the individual mitochondrium and total volume of mitochondria as well as an increased lipoid content together with a more pronounced dispersion of liposomes. The same function-specific structures show a further increase in females during estrus. Castration of females on the 4th day of life leads to a reduction of the function-specific morphometric parameters below the values of the diestrus animals. Male castrates show adaption of the values of their function-specific structures towards those of females during diestrus. Following castration on the 4th day of life, female animals thus show somewhat lower morphometric parameters in the outer zona fasciculata than male castrates.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00209054
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    The fine structure of adrenal zona glomerulosa in the adult rat (1974)
    Springer
    Cell & tissue research 149 (1974), S. 235-243 
    Details
    ISSN: 1432-0878
    Keywords: Adrenal zona glomerulosa ; Rat ; Normal ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary This paper reports on an electron microscopic study of the adrenal zona glomerulosa in the normal rat, with special emphasis on the structural diversity of mitochondria in its parenchymatous cells. The mitochondria of cristae may be tubulo-vesicular, plate-like and/or straight tubular, any one mitochondrion containing one or more types of cristae. The mitochondrial features of the zona glomerulosa parenchymatous cells are attributed to the biochemical stages of aldosterone synthesis, taking place in different mitochondria of these cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222276
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  • 62
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    Different populations of granules and their distribution in the hypothalamo-neurohypophysial tract of the rat under various experimental conditions (1974)
    Springer
    Cell & tissue research 151 (1974), S. 117-140 
    Details
    ISSN: 1432-0878
    Keywords: Hypothalamo-neurohypophysial system ; Rat ; Granule distribution ; Electron microscopy, morphometry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Die Neurohypophyse und die neurosekretorischen hypothalamischen Kerngebiete von normalen männlichen und weiblichen Ratten, von trächtigen und durstenden Tieren wurden elektronenmikroskopisch und morphometrisch mit der Fragestellung untersucht, ob sich in den Häufigkeitsverteilungen der mittleren Granula-Durchmesser, bezogen auf die Anzahl der Axone, eine zweigipflige Verteilung ergibt. Bei Kontrolltieren zeigen die Perikarya des Nucleus supraopticus zahlreichere und größere Granula als die Zellen des Nucleus paraventricularis, wobei der Unterschied in den Durchmessern der Granula bei den weiblichen Tieren ausgeprägter ist als bei den männlichen. Beide Kontrollgruppen zeigen in der Neurohypophyse ein zweigipfliges Verteilungsmuster der Granula, wobei die beiden Gipfel bei den männlichen Kontrollen dichter beieinander liegen als bei den weiblichen. Bei den trächtigen Tieren weisen beide Kerngebiete Zeichen gesteigerter Aktivität auf und die Granula in den Zellen des Nucleus paraventricularis sind deutlich größer als bei Kontrolltieren. Zu Beginn der Tragzeit werden relativ große, wahrscheinlich vasopressinhaltige Granula in der Neurohypophyse gespeichert, während sich im letzten Drittel der Tragezeit die beiden Populationen dort nicht mehr unterscheiden lassen. Nach 48 Std. Wasserentzug erscheinen beide hypothalamischen Kerne aktiviert. Auffallend ist, daß die Granula im Nucleus supraopticus deutlich kleiner sind als bei Kontrolltieren. Entsprechend fehlen in der Neurohypophyse große Granula, während Axone mit kleinen Granula vermehrt sind. Nach zehntägiger Durstperiode lassen sich im Nucleus supraopticus, neben sehr typischen morphologischen Veränderungen, morphometrisch ebenfalls deutlich kleinere Granula nachweisen. Im Hypophysenhinterlappen dieser Versuchsgruppe sind die granulahaltigen Axone sehr stark vermindert, und in der Häufigkeitsverteilung fehlen die Granula mit großem Durchmesser während der dem Oxytocin zugeordnete Gipfel unverändert erscheint. In Verbindung mit den elektronenmikroskopischen Befunden lassen sich also aus den Verschiebungen der Verteilungsmuster zwischen den sechs Versuchsgruppen die Granula mit kleinerem Durchmesser oxytocinhaltigen Axonen zuordnen und Granula mit größerem Durchmesser zu den Endigungen, die Vasopressin enthalten. Die morphometrischen Befunde in den neurosekretorischen hypothalamischen Kerngebieten bestätigen diese Zuordnung. Die elektronenmikroskopischen und morphometrischen Befunde werden besonders im Hinblick auf die Hormonverschiebungen zwischen dem “reserve pool” und den “easily releasable pool” diskutiert.
    Notes: Summary A morphometric evaluation of electron micrographs has been carried out from neurosecretory terminals in the neurohypophysis and from the supraoptic and paraventricular nuclei in normal male and female rats as well as in pregnant and water deprived rats. The task of this investigation was to find out whether frequency distribution diagrams of the mean diameter of the neurosecretory granules, plotted versus the number of axons, reveal a grossly bimodal distribution. In normal rats the nerve cells of the nucleus supraopticus (S.O.N.) show more numerous and larger granules than the cells of the nucleus paraventricularis (P.V.N), this difference with respect to the diameter being more pronounced in female than in male animals. In the posterior pituitary lobes the neurosecretory granules exhibit a bimodal distribution, the second peak being situated more closely to the first one in male controls. In pregnant animals both nuclei appear to be activated, and the granules of P.V.N. are distinctly larger as compared to those of female control animals. In early pregnancy larger, i.e. presumably vasopressin-containing granules are stored in the neurohypophysis, while in the last phase before delivery oxytocin-containing neurons and vasopressin-containing ones cannot be differentiated any more. After 48 hrs of thirst both hypothalamic nuclei exhibit signs of increased activation, the diameters of the granules in the S.O.N. being distinctly smaller than in normals. In the neurohypophysis granules with large diameters are lacking, while axons with small granules are more frequent. In the S.O.N. of animals, water deprived for 10 days the diameters of the granules are smaller than normal and in the neurohypophysis the number of granule-containing fibres is strongly reduced. In the frequency distribution diagram granules with larger diameters are lacking whereas the peak, attributed to oxytocin, is not essentially altered. However, from the observed alteration on the distribution patterns of the six experimental groups and in connection with the electron microscopic results the granules with smaller diameters can be attributed to oxytocin-containing axons and the larger ones to vasopressin-containing terminals. Analogous morphometric data have been obtained from the neurosecretory nuclei as well. The electron-microscopic and morphometric observations are discussed with regard to hormone-shifts between the reserve pool and the easily-releasable pool.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222039
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    Studies on the juxtaglomerular apparatus (1974)
    Springer
    Cell & tissue research 151 (1974), S. 471-480 
    Details
    ISSN: 1432-0878
    Keywords: Juxtaglomerular apparatus ; Rat ; Influence of deoxycorticosterone (DOC) ; Electron microscopy ; Morphometric analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Die quantitativ-morphologische Analyse des juxtaglomerulären Apparates nach DOC-Behandlung zeigte, daß mit der Hemmung der Reninbildung eine Hypoplasie der epitheloiden Zellen des juxtaglomerulären Apparates einhergeht. Der Granulagehalt der juxtaglomerulären Zellen nahm ab; die Kern-Plasma-Relation änderte sich und die Leiomyofibrillen in den Epitheloidzellen nahmen zu. An den übrigen Anteilen des juxtaglomerulären Apparates, insbesondere der Macula densa, wurden keine morphologischen Veränderungen beobachtet. Die DOC-Behandlung führte darüber hinaus zu einer Bildung von Einschlüssen in den Podocyten, den Mesangiumzellen und den Zellen der Bowman-Membran. Die verschiedenen Möglichkeiten, über welche Faktoren die juxtaglomerulären Zellen nach DOC-Behandlung degranuliert werden, werden diskutiert. Das ultrastrukturelle Bild der Epitheloidzellen nach DOC-Behandlung entspricht deutlich einer gehemmten Sekretion, da das Ergastoplasma und der Golgi-Apparat ganz besonders stark zurückgebildet werden.
    Notes: Summary Quantitative morphological analysis of the Juxtaglomerular apparatus of rats treated with deoxycorticosterone (DOC) and salt revealed hypoplasia of the epithelioid cells as a concomitant of the inhibition of renin synthesis. The granular content of the Juxtaglomerular cells was significantly decreased, while the nucleus-to-cytoplasm ratio and the volume density of leiomyofilaments in the epithelioid cells increased. No morphological alterations were observed in the remaining constituents of the Juxtaglomerular apparatus, including the macula densa. DOC-treatment also induced the appearance of inclusions in the podocytes in the cells of the mesangium, and in the cells of Bowman's capsule. The possible mechanisms of degranulation of the Juxtaglomerular cells after treatment with DOC are discussed. The ultrastructural appearance of the epithelioid cells after treatment with DOC corresponds to an inhibition of secretion, since the rough endoplasmic reticulum and the Golgi apparatus undergo marked regressive changes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222992
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    Einfluß der Splenektomie auf Magensaftsekretion und Ulcusentstehung der Ratte (1974)
    Springer
    Langenbeck's archives of surgery 336 (1974), S. 15-23 
    Details
    ISSN: 1435-2451
    Keywords: Rat ; Splenectomy ; Gastric Ulceration ; Increase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung An Ratten wurde der Einfluß von Splenektomie auf Magensaftsekretion und Ulcusentstehung untersucht. Sowohl nach “restraint-stress” als auch Pylorusligatur wiesen die splenektomierten Tiere eine ausgeprägte Steigerung der Ulcuszahl und -größe gegenüber den Kontrolltieren auf. In der Magensaftanalyse waren dagegen die Werte der splenektomierten Tiere gegenüber den scheinoperierten nur geringgradig gesteigert. — Als mögliche Ursache der vermehrten Ulcusanfälligkeit nach Splenektomie wurde eine Veränderung lokaler, nicht säuresekretorischer Faktoren der Magenwand diskutiert.
    Notes: Summary In Splenectomiced rats incidence of gastric ulceration was compaired with control groups. Restraint stress and ligation of pylorus was used as model. Splenectomiced rats do show an higher incidence in both models. Both frequency and extension of gastric ulcer were accumulated significantly. Analysis of gastric secretion was only unessentially raised in comparisom to control-groups. Change of protective factors of stomach as possible reason is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01291870
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    Metabolisches Verhalten von kolloidalem Ferrihexacyanoferrat(II) (1971)
    Springer
    Naunyn-Schmiedeberg's archives of pharmacology 269 (1971), S. 48-56 
    Details
    ISSN: 1432-1912
    Keywords: Colloidal Ferrihexacyanoferrate(II) ; Potassium Hexacyanoferrate(II) ; Metabolism ; Toxicity ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Intestinal absorption, distribution, and excretion of ferrihexacyanoferrate(II), labeled by59Fe at the Fe(III)- and Fe(II)-atoms, respectively, was studied in rats. Following i.v. injection, the compound is separated at ∼60% into Fe3+ and [Fe(CN)6]4−, the residual fraction being retained by the reticuloendothelial system. [Fe(CN)6]4− is excreted rapidly and virtually completely by the kidneys. With oral administration the disintegration amounts to ∼7%, and ∼2% of [Fe(CN)6]4− is absorbed from the gut. No evidence was obtained for decomposition of [Fe(CN)6]4−. No toxic side-effects were observed after chronic administration of ferrihexacyanoferrate(II).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01422015
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  • 66
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    Ultrastructure of rat uterine luminal epithelium at functional states compatible with implantation (1971)
    Springer
    Anatomy and embryology 135 (1971), S. 101-107 
    Details
    ISSN: 1432-0568
    Keywords: Rat ; Uterus ; Luminal epithelium ; Ultrastructure ; Implantation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The ultrastructure of the rat uterine luminal epithelium has been examined during normal pregnancy, pseudopregnancy, lactation and lactation-pregnancy, all being conditions when a blastocyst is known to be able to implant. In all four groups of animals the epithelium passes through three characteristic and structurally similar states, namely a pre-attachment state, an attachment state and a postattachment state. The attachment state is characterized by a close contact between apposing luminal surfaces of the epithelium. It is concluded that implantation in an undamaged uterus claims a sequence of certain changes in the uterine luminal epithelium, and that the blastocyst attachment occurs within the early part of that period when the luminal epithelium is in the attachment state.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00525195
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  • 67
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    Licht-und elektronenmikroskopische Untersuchungen zum Problem der Blut-Hirn-Schranke circumventriculärer Organe der Ratte nach Behandlung mit Myofer (1971)
    Springer
    Anatomy and embryology 134 (1971), S. 278-297 
    Details
    ISSN: 1432-0568
    Keywords: Rat ; Ferro-dextran ; Blood brain barrier ; Circumventricular organs
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Nach Vorbehandlung mit Myofer (Eisen III-Dextran) wurde die Aufnahme der Testsubstanz in den circumventriculären Organen von Rattengehirnen mit dem Licht-und Elektronenmikroskop untersucht. Die Ergebnisse zeigten, daß ein partieller Schrankeneffekt zwischen Gefäß und Gehirn auch in diesen Regionen besteht. Im allgemeinen ist der Schrankeneffekt die Folge hintereinandergeschalteter Barrieren, deren morphologisches Substrat 1. das Gefäßendothel und 2. die Gliagrenzschicht sind. Im Parenchym der verschiedenen Gehirnteile ist die Endothelzelle für diese gegebene Substanz undurchlässig. Dabei wird Myofer zwar in die Zelle aufgenommen, aber total zurückgehalten. In den circumventriculären Organen des Rattengehirns (Area postrema, Epiphyse, Subfornikalorgan, Organum vasculosum laminae terminalis, Neurohypophyse, Plexus chorioidei) ist die Endothelzelle durchlässig. Daher findet man Myoferpartikel im perivasculären Raum. Der. „Hemmeffekt” der zweiten Barriere ist abhängig 1. von der Konzentration des Myofers in dem perivasculären Raum und 2. von der Zeitdauer, während der die Gliagrenzschicht beeinflußt wird. Die perivasculären Gliafortsätze nehmen als einzige Zellelemente Myofer auf. Da der Anteil von Zellen mit Gliacharakter in den circumventriculären Organen wechselt, ist in den einzelen Regionen Myofer in unterschiedlicher Menge nachweisbar. Neuronale Elemente enthalten niemals Myofer.
    Notes: Summary After pretreatment of rats with Myofer (ferro-dextran complex) the uptake of this substance into circumventricular organs of the brain has been studied by means of light and electronmicroscopy. The results show that in these regions a partial barrier effect exists between blood and brain parenchyma. Generally the barrier effect is the result of impediments, which are connected in series. The morphological substratum of them is (1) the endothelium of the capillaries and (2) the limiting layer of the glial processes. In the parenchyma of the brain (cerebellum) the endothelial cell is sealed for this substance: the Myofer is taken up by the endothelium, but retained in the cells. In the circumventricular organs of the rat's brain (area postrema, epiphysis cerebri, subfornical organ, Organum vasculosum laminae terminalis, neurohypophysis, choroid plexus) the endothelial cells are leaky. Consequently one finds particles of Myofer inside the perivascular space. The inhibitory effect of the second barrier depends (1) on the concentration of Myofer in the perivascular space and (2) on the period, during which the limiting layer of the glial processes has been affected. The perivascular glial processes are the only cell elements which take up Myofer. Since the proportion of cells characterized as glial elements changes between the circumventricular organs, Myofer is detectable in different amounts within the various regions. Neuronal elements never contain Myofer.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00519916
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    Electron microscopic observations on the development of the median eminence in perinatal rats (1971)
    Springer
    Anatomy and embryology 134 (1971), S. 311-327 
    Details
    ISSN: 1432-0568
    Keywords: Development ; Median eminence ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary This paper reports details of the development of the external layer of the median eminence with special reference to the appearance of the nerve terminals, containing electron dense core and electron lucent vesicles, in the outermost layer of a palisade structure (2–3 μ thick). Observations were made on perinatal rats of the Sprague-Dawley strain (16, 18, 20 day fetuses and rats 5 hours, one 4, and 8 days after birth). The palisade structure of the outermost layer of the median eminence was first clearly detectable 5 hours after birth. Concomitantly, there was a marked increase in the number of nerve terminals in this layer. The nerve terminals containing electron dense core and electron lucent vesicles were seen in 18 day fetuses in the outermost layer of the median eminence. These nerve terminals increased in number during development, especially from the 20th day of fetal life to the 4th day after birth. The size range of the majority of the electron lucent vesicles did not change during development, but that of the core vesicles increased from 600–800 Å from the 18th to 20th day of fetal life. The initiation of neurohemal control of the adenohypophysial function by the hypothalamus is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00519918
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  • 69
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    Effects of60Co radiation on the cellular responses in degenerating dorsal columns in the rat spinal cord (1971)
    Springer
    Acta neuropathologica 19 (1971), S. 318-322 
    Details
    ISSN: 1432-0533
    Keywords: Co-Radiation ; Spinal Cord ; Rat ; Cell Proliferation ; p-Bromophenylacetylurea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Rats were irradiated with doses of 1 000–3 000 rads to the cervical spinal cord and subsequently given a paralytic dose of p-Bromophenylacetylurea. The nuclear populations in the degenerating dorsal columns were determined and it was found that a significant suppression of cell proliferation occurred after all three dose levels. The cell populations in the shielded parts of the tracts rostral to the irradiated zone were not affected.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00692152
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    Über die akuten Veränderungen im Parietalcortex der Ratte nach spitzem Trauma (1971)
    Springer
    Acta neuropathologica 19 (1971), S. 249-264 
    Details
    ISSN: 1432-0533
    Keywords: Brain Edema ; Stab Wound ; Neocortex ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The parietal cortex of rats was examined by light and electron microscopy 1–120 min after a standardized stab wound (250×450×1800μm, constant stab velocity). The changes in the tissue are already visible qualitatively after 1 min. After about 4 min the expansion of tissue changes stops. 4 zones may be separated. Surrounding the stab canal concentrically they are relatively sharply defined. Zone I. Stab canal, haemorrhagical or “debris zone”, primary traumatic destroyed zone. The tissue units are here completely destroyed. Zone II. “Squashed” or “indirectly but irreversibly damaged” zone. It is ca. 80μm wide and contains apart from a protein-rich extracellular fluid almost exclusively swollen cells and cell fragments. Zone III. Swelling brain or “intracellular peritraumatic edema”. It is ca. 150μm wide and contains mainly dark neurones and swollen astroglia. Zone IV. Transitional zone of variable width. Here only the perivascular and perineural processes are swollen without changed neurone structure. In all swollen astrocytes mitochondria are altered typically (denser matrix, dilated cristae). Discussed are: The short latency period, Zone IV, causes of astroglial swelling, origin of extracellular fluid as well as mechanisms which limit the spread of extracellular fluid into Zone II.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00684602
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    An anatomical study of the efferent connections of the thalamic reticular nucleus (1971)
    Springer
    Experimental brain research 12 (1971), S. 435-446 
    Details
    ISSN: 1432-1106
    Keywords: Thalamic reticular nucleus ; Ascending reticular system ; Topo graphical arrangement ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A neuro-anatomical investigation into the thalamic reticular nucleus and its connections was made in the albino rat, using the method of retrograde degeneration and the Nauta-Gygax method. The results correspond to the description given by Scheibel and Scheibel and indicate connections of the neurons of the reticular which the principal thalamic nuclei and the rostral part of the intralaminar thalamic nuclei. A topographical arrangement of the efferent connections of the reticular nucleus is demonstrated. The projection of the reticular nucleus upon the thalamus duplicates the cortico-thalamic connections.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00234497
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    Effects of chronic amphetamine intoxication on the accumulation in the rat brain of labelled catecholamines synthesized from circulating tyrosine-14C and dopa-3H (1971)
    Springer
    Naunyn-Schmiedeberg's archives of pharmacology 271 (1971), S. 211-233 
    Details
    ISSN: 1432-1912
    Keywords: Amphetamine ; Synthesis of Catecholamines ; Brain Catecholamines ; Release of Catecholamines ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Accumulation of labelled noradrenaline (NA) and dopamine (DA) and endogenous levels of NA and DA in brain and heart were measured after infusion of tyrosine-14C and dopa-3H in rats. After a single dose of 20 mg/kg i.p. of dl-amphetamine sulphate a 50–65% decrease in the NA accumulation in the brain and heart was observed. The accumulation of DA was not changed. In rats treated chronically with amphetamine sulphate, 16−32 mg/kg i.p. twice daily for 4 weeks, both the brain and heart NA and brain DA levels were depleted by 40–70% at 20–24 h after amphetamine withdrawal. The accumulation of labelled NA and DA in these animals was not different from that of saline treated controls. When amphetamine sulphate, 20 mg/kg i.p., was given to chronically amphetamine treated rats there was a 45–60% decrease in the accumulation of labelled NA; labelled DA remained unchanged. It is concluded that the decrease in NA accumulation under acute and chronic amphetamine intoxication is most likely due to a preferential release of newly synthesized NA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00997217
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    Effect of chlorpromazine on conditioned avoidance as a function of CS-US interval length (1971)
    Springer
    Psychopharmacology 22 (1971), S. 144-150 
    Details
    ISSN: 1432-2072
    Keywords: Avoidance ; Chlorpromazine ; CS-US Interval ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Three groups of four rats learned to avoid electric shock (US) by turning a wheel in response to a buzzer stimulus (CS). The CS-US intervals for each group were 5, 10, and 20 sec, respectively. After each animal had learned the avoidance procedure and had achieved a stable level of performance, the effect of several doses of chlorpromazine on percent avoidance, on latency time from CS onset to termination by a response, and on response rate was determined as a function of CS-US interval length. No consistent relationship between increasing interval length and response rate was observed. Neither lengthening the CS-US interval nor the interaction of this lengthening with chlorpromazine dose was found to exert a statistically significant effect on percent avoidance. Furthermore, although a statistically significant increase in response latency was found to be associated with increasing CS-US interval length, the increases in latency noted were not of sufficient magnitude to corroborate the hypothesis that lengthening the CS-US interval contributes importantly to increased avoidance responding in animals tested with chlorpromazine. Further, the results of this study do not support induction of a locomotor deficit as the mechanism by which chlorpromazine suppresses the avoidance response.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00403622
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    The effects of chronic administration of ethanol on startle thresholds in rats (1971)
    Springer
    Psychopharmacology 19 (1971), S. 95-104 
    Details
    ISSN: 1432-2072
    Keywords: Ethanol ; Tolerance ; Dependence ; Withdrawal Reaction ; Startle Threshold ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The thresholds for startle responses to electric shock were measured in adult male Wistar strain rats given ethanol daily in doses rising from 3 to 7 g/kg over a 30-day period, and in controls receiving equicaloric doses of sucrose. Tests made 23, 36, or 47 h after ethanol (i.e., during partial or complete ethanol withdrawal) gave threshold values significantly lower than those obtained with sucrose-treated controls. The difference became greater after longer ethanol treatment and larger doses. However, when threshold measurements were made under the acute influence of ethanol in the experimental group, the mean values were virtually equal to those of the sucrose controls. This normalization, by ethanol, of a disturbance produced by absence of ethanol in a chronically treated animal is indicative of physical dependence. Following termination of ethanol treatment there was a gradual return of startle thresholds almost to control values over a relatively short period, indicating that the changes underlying the hyperexcitability are readily reversible.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00402633
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    Untersuchungen über die Stereotypien nach Amphetamin und Apomorphin sowie deren pharmakologische Beeinflussung (1971)
    Springer
    Psychopharmacology 21 (1971), S. 212-228 
    Details
    ISSN: 1432-2072
    Keywords: Amphetamine ; Apomorphine ; Stereotypy ; Behaviour ; Rat ; Mouse ; Neuroleptics ; Thymoleptics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The behaviour of mice after various doses of amphetamine or apomorphine which induce stereotypies is described in details. The induced stereotyped behaviour in mice appears more differentiated than that in rats. The amphetamine-syndrome in rats consists of an excitement phase followed by the stereotypy, whereas apomorphine elicits stereotyped behaviour directly after administration. Amphetamine-induced stereotyped behaviour is different from the apomorphine-induced stereotypy in several respects. Neuroleptics inhibit the amphetamine (12.5 mg/kg s.c.) and apomorphine-(10 mg/kg s.c.) syndrome, while thymoleptics potentiate subeffective doses of amphetamine (5 mg/kg s.c.) and apomorphine (2 mg/kg s.c.). This is demonstrated with some neuroleptics of the phenothiazine-type, haloperidol, reserpine, and a group of thymoleptics. The method opens the possibility of differentiating within several groups of neuroleptics and within several groups of thymoleptics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00403860
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  • 76
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    The effect of two tetrahydrocannabinols, (Δ9-THC and Δ8-THC) on conditioned avoidance learning in rats and its transfer to normal state conditions (1971)
    Springer
    Psychopharmacology 22 (1971), S. 23-30 
    Details
    ISSN: 1432-2072
    Keywords: Acquisition ; CAR ; Tetrahydrocannabinols ; Transfer ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Rats trained in conditioned avoidance responding (CAR) after injections of either 7.5 mg/kg Δ 9-THC (tetrahydrocannabinol) or 15 mg/kg Δ 8-THC, showed no transfer when tested in the non-drugged state. Furthermore, these doses of the isomeric tetrahydrocannabinols exerted a disruptive effect on previously established CAR in rats, trained under normal conditions. Only the Δ 9-THC-group showed an impairment of acquisition which was statistically significant compared to the control group.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00401463
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  • 77
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    The effects of cholinergic agents upon fixated behavior (1971)
    Springer
    Psychopharmacology 21 (1971), S. 147-156 
    Details
    ISSN: 1432-2072
    Keywords: Scopolamine ; Pilocarpine ; Lashley Jumping Stand ; Fixated Behavior ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract A group of male albino rats were subjected to the Maier paradigm (insoluble problem followed by a soluble problem) using the Lashley jumping stand. Forty-two animals which failed the soluble problem by adopting a position stereotype were then randomly assigned to eight drug groups in a 2×2 design. Animals were guided to the correct window on odd days but received no guidance on even days. Animals received either pilocarpine nitrate (5.0 mg/kg) or scopolamine hydrobromide (1.0 mg/kg) in one of three different sequences. These sequences included drug on both odd and even days (drug-drug), only on the odd day (drug-no drug), or only on the even day (no drug-drug). One other drug group received scopolamine methylbromide (1.0 mg/kg) in a drug-no drug sequence, while the control group received saline on both days. Results indicated that animals receiving pilocarpine in the drug-drug and no drug-drug sequence solved significantly faster than the controls, while all the drugno drug groups showed significantly poorer solution rates. It was concluded that pilocarpine may enable animals to inhibit punished behavior patterns and thus hasten the extinction of fixated responses, but that due to the inconclusive scopolamine data this pilocarpine effect may not be due to its cholinomimetic properties.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00572272
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  • 78
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    Biochemical evidence of adrenergic interaction with cholinergic function in the central nervous system of the rat (1971)
    Springer
    Psychopharmacology 21 (1971), S. 238-246 
    Details
    ISSN: 1432-2072
    Keywords: NE ; Intraventricular ; Cholinergic Function ; Eating Behaviour ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The effect of centrally administered norepinephrine (NE) into the lateral ventricle on choline acetylase activity (ChAc), endogenous levels of NE, dopamine (DA) and serotonin (5HT) was studied in different regions of the rat brain. In normal rats, the highest activity of choline acetylase was found in the cerebral cortex, followed by the brain stem, the diencephalon, the hypothalamus and least in the cerebellum. Chronic administration of NE significantly increased the choline acetylase activity in all regions studied, whereas both the acute and the in vitro studies showed no significant change except in the brain stem. Serotonin level was increased in the cerebellum, but decreased in the diencephalon and the brain stem. There was no significant alteration in the level of NE in all areas studied except in the hypothalamus where there was an increase in the mean concentration. Tissue level of DA showed a significant increase in the cerebral cortex and the hypothalamus. Behaviourally, there was a significant increase in food intake on the first day of treatment with no significant change in water intake. The data suggests that NE may be involved in the regulation of acetylcholine synthesis. The significance of interaction between different monoamines and acetylcholine may be important in the study of drug tolerance phenomena.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00403862
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  • 79
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    Role of γ-aminobutyric acid (GABA) in the extrapyramidal motor system 1. Regional distribution of GABA in rabbit, rat, guinea pig and baboon CNS (1971)
    Springer
    Experimental brain research 13 (1971), S. 514-518 
    Details
    ISSN: 1432-1106
    Keywords: GABA-Regional distribution ; Rabbit ; Rat ; Guinea pig ; Baboon ; CNS ; Substantia nigra
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The regional distribution of GABA in the CNS of rabbit (17 regions), rat (14 regions), guinea pig (12 regions) and baboon (11 regions) was determined by sensitive enzymatic and fluorometric assay. In all species studied a high concentration of GABA was found in substantia nigra (8.5–10.1 mmoles/kg) and pallidum (6.5–8.2 mmoles/kg). Fairly high amounts were found in hypothalamus, superior and inferior colliculi, nucleus oculomotorius and dentate nucleus. The GABA concentration in spinal cord (white matter) had the lowest value.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00234282
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  • 80
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    NaK stimulated adenosinetriphosphatase: Intracellular localisation within the proximal tubule of the rat nephron (1971)
    Springer
    Pflügers Archiv 330 (1971), S. 265-270 
    Details
    ISSN: 1432-2013
    Keywords: NaKATPase ; Intracellular Localisation ; Proximal Tubule ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary With refinements of quantitative histochemistry, i.e. oilwell technique, enzymaticP ianalysis and NADP/NADPH cycling an enzymatic polarization of the tubular epithelial cell from the rat nephron to NaKATPase can be established. NaKATPase activity is limited to the basal area of the epithelial cell. Brush border fragments lack NaKATPase activity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00588617
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  • 81
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    Influence de l'isolement sur le comportement d' agressivité intraspécifique induit par l'apomorphine chez le rat (1971)
    Springer
    Psychopharmacology 20 (1971), S. 389-394 
    Details
    ISSN: 1432-2072
    Keywords: Aggressive Behaviour ; Apomorphine ; Isolation ; Psycho pharmacology ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The apomorphine-induced aggression response of isolated rats was examined. The development of aggressive behaviour in adult rats was enhanced after isolation in cages with wire-netting and especially in cages isolated in opacified Makrolon boxes compared with aggregated rats. Young rats kept isolated for 2 months immediately after weaning showed an enhancement only if isolated in wire-netting cages. Rats kept in groups for one month after their isolation still showed an enhancement of aggression, although it was somewhat reduced.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00403570
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  • 82
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    Central action of phentolamine administered intraventricularly in the rat (1971)
    Springer
    Psychopharmacology 20 (1971), S. 348-354 
    Details
    ISSN: 1432-2072
    Keywords: Phentolamine ; Noradrenaline ; Amphetamine ; Rat ; Locomotor Activity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Phentolamine injected intraventricularly in unanaesthetized rats decreased their locomotor activity and antagonized the excitatory effect of intraventricularly injected noradrenaline, or amphetamine injected s.c. Phentolamine did not influence the level of noradrenaline and 5-hydroxytryptamine in the rat brain. The mechanism of observed action of phentolamine is discussed.
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    URL: http://dx.doi.org/10.1007/BF00403566
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  • 83
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    The effects of acutely administered fenfluramine on activity and eating behaviour (1971)
    Springer
    Psychopharmacology 21 (1971), S. 165-173 
    Details
    ISSN: 1432-2072
    Keywords: Anorexic Effect ; Fenfluramine ; Activity Analysis ; Time Sampling Technique ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Three experiments are reported investigating the effects of acute administration of fenfluramine over 3 doses on activity, eating and drinking behaviour. A time sampling procedure of activity analysis was used, employing six behaviour categories. Fenfluramine produced a dose related decrease in rearing behaviours, with some evidence of an increase in walking categories at the lower dose levels. Eating and drinking behaviours showed clear dose related decreases. Sniffing categories showed a clear dose related increase. Attention is drawn to some of the difficulties of interpretation and assessment of anorexic effect.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00572274
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  • 84
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    The influence of 1-(o-Allylphenoxy)-3-Isopropylamino-2-Propanol Hydrochloride (Alprenolol) on the central nervous system of the rat (1971)
    Springer
    Psychopharmacology 21 (1971), S. 66-73 
    Details
    ISSN: 1432-2072
    Keywords: Alperenolol ; Amphetamine ; Brain Amines ; Behaviour ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The influence of alprenolol and amphetamine alone or combined on the content of noradrenaline, dopamine and 5-hydroxytryptamine in seven discrete areas of the brain and on rat behaviour was studied. Animals were treated with drugs for 6 months. Alprenolol caused mainly a decrease of the estimated endogenous amines in different brain areas. Amphetamine caused a decrease of all three amines in some parts of the brain, and reversed some of the changes caused by alprenolol. Alprenolol had no effect on the locomotor activity of rats, but increased the activity of rats treated with amphetamine after the first week of treatment, and antagonized the excitatory effect caused by amphetamine during the following weeks of the experiment.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00403997
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    Der Einfluß der Durchblutung auf die Resorption von Polyalkoholen aus dem Jejunum der Ratte (1971)
    Springer
    Naunyn-Schmiedeberg's archives of pharmacology 270 (1971), S. 22-40 
    Details
    ISSN: 1432-1912
    Keywords: Intestinal Blood Flow ; Intestinal Absorption ; Polyalcohols ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary 1. Jejunal loops of anaesthetized rats were perfused with14C-labelled polyalcohols in buffered saline solutions at pH 7. The blood flow, the disappearance rate (from the intestinal lumen), and the appearance rate (in the intestinal venous blood) were determined simultaneously. 2. A decrease of the blood flow from about 1.8 to 0.25 ml min−1g−1 wet tissue diminished the disappearance and appearance rate of the substances and their possible metabolites. An increase of the blood flow caused the reversed results. The two transfer rates and their dependence on blood flow decreased with increasing molecular weight of the polyalcohols. 3. The appearance rate of glycerol showed a different dependence on blood flow in experiments with increasing and decreasing blood flow. This phenomenon can be explained by a decreasing permeability of the epithelium for glycerol with time. The cause for it is unknown. 4. By means of a three-compartment-model blood flow independent permeability coefficients of the polyalcohols are derived for the intestinal epithelium. They are consistent with an equivalent pore radius of 3.9±0.1 Å.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00997297
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    On the innervation of the rat and pig adrenal cortex (1971)
    Springer
    Cell & tissue research 116 (1971), S. 151-156 
    Details
    ISSN: 1432-0878
    Keywords: Adrenal cortex ; Innervation ; Receptors ; Rat ; Pig
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The innervation of the adrenal cortex of the rat and the pig is investigated with the electron microscope. Nerve fibers containing synaptic and two types of dense-cored vesicles come into contact with endocrine cells. There are no specialized pre- and postsynaptic membranes. The synaptic cleft is about 200 Å wide. Generally the basement membrane between nerve and cell is absent. These observations are discussed on the base of more recent experimental findings. Small fibers having an average diameter of about 0.2 to 0.5 μ and containing only tubules and filaments are considered to represent parts of an afferent nervous system.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00332863
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  • 87
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    Histochemical and chemical studies on pre- and postnatal development of the different systems of “short” and “long” adrenergic neurons in peripheral organs of the rat (1971)
    Springer
    Cell & tissue research 116 (1971), S. 319-341 
    Details
    ISSN: 1432-0878
    Keywords: Adrenergic neurons ; “Short” and “long” systems ; Pre- and postnatal development ; Peripheral organs ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The adrenergic innervation in the submaxillary gland, heart, kidney, small intestine, and accessory male genital organs and the development of the adrenal chromaffin cells and the sympathetic ganglia were studied in the rat from 15 days post coitum to 16 days post partum using the fluorescence histochemical method of Falck and Hillarp. The postnatal development of the noradrenaline concentrations in the heart and vas deferens was followed by fluorometric determinations. At about 15 days post coitum, the anlagen of the sympathetic chains were well visible in the form of two dorsal segmented columns of small branching sympathicoblasts exhibiting an intense catecholamine fluorescence. In the midline, ventrally to these two anlagen, another column of sympathicoblasts developed; this seemed to give rise to the prevertebral ganglia and to the short adrenergic neurons supplying the internal genital organs. At the level of the adrenal anlagen, small intensely fluorescent chromaffin cells were collected in two bilateral groups which became enclosed by adreno-cortical cells. This enclosure was, however, not complete even at two weeks post partum. Bundles of growing sympathetic nerves were visible in the periphery of the various organs studied at 19–21 days post coitum. A terminal innervation of the organs suggestive of a functional transmitter mechanism did not start to establish until at or immediately after birth. The final pattern of innervation was usually reached at about one week post partum, and the following development proceeded largely in the form of a quantitative increase in the number of nerves participating in the innervation apparatus. The adult level of noradrenaline in the heart and vas deferens was reached three to five weeks after birth. The small intestine was an exception in that the final pattern of innervation in the wall was attained immediately after birth. There was no overt difference in the rate of development of the terminal sympathetic innervation in organs supplied by short adrenergic neurons (accessory male genital organs) compared to the innervation of the submaxillary gland, heart and kidney, which receive classical long adrenergic neurons.
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    URL: http://dx.doi.org/10.1007/BF00330631
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    The fine structure of the granulosa cells in the domestic fowl and the rat (1971)
    Springer
    Cell & tissue research 119 (1971), S. 58-67 
    Details
    ISSN: 1432-0878
    Keywords: Ovary ; Rat ; Fowl ; Granulosa cells ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The granulosa cells of the ovarian follicle of the rat and the domestic fowl have been studied with the light and electron microscope. The nuclei of the granulosa cells were irregular with indentations and large in proportion to the cytoplasm of the cell. The mitochondria had a dense, dark matrix with only few cristac. The Golgi apparatus was moderately developed, located towards the oocyte in a juxtanuclear position. The endoplasmic reticulum was rather sparse. Lipid droplets were only occasionally encountered. Microtubules were regularly observed. The functions of the granulosa cells are discussed. Compared with the steroid-producing cells of the theca interna of the same follicles, the granulosa cells primarily are the nursing cells for the growing oocyte and mainly have the characteristics of protein forming cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00330538
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    Ultrastructural studies on the hypothalamic neurosecretory neurones of the rat (1971)
    Springer
    Cell & tissue research 122 (1971), S. 283-300 
    Details
    ISSN: 1432-0878
    Keywords: Paraventricular nucleus ; Rat ; Synthesis of hormones ; Acid phosphatase ; Osmium impregnation ; Neurosecretion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The ultrastructural features of the paraventricular neurones of the non-treated rat are presented comparing them with those of the supraoptic neurones. No striking differences are seen between the general electron microscopic characteristics of the paraventricular and supraoptic neurones. The importance of adequate fixation to obtain good preservation of the neurones is emphasized, since inadequate fixation can cause e.g. artefactual appearance of “dark neurones”. The previously presented classification of the neurosecretory neurones into two categories (e.g. “light and dark neurones”) on the basis of the number of ribosomes is not considered justifiable, since their number can vary to a very great extent even within a single cell. The synthesis of neurosecretory products in the paraventricular neurones obviously follows the general mode of the synthesis of secretory proteins: ribosomes—RER—Golgi complex—secretory vesicles. On the basis of the localization of heavy metal deposits after osmium impregnation and demonstration of acid phosphatase the Golgi complex of the paraventricular neurones is found to be polarized. The direction of the polarity is discussed. The substructures of the dense cores of the neurosecretory granules and of the contents of the lysosomal dense bodies are nearly identical. Therefore it is considered impossible to determine positively the nature of the dark condensed material within the Golgi complex. The characteristics of the immature neurosecretory granules and the possibility of releasing neurosecretory products into the cytoplasm already within the perikarya are speculated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00935990
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  • 90
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    A two-filament system and interaction of heavy meromyosin (HMM) with thin filaments in smooth muscle (1971)
    Springer
    Cell & tissue research 122 (1971), S. 350-356 
    Details
    ISSN: 1432-0878
    Keywords: Smooth muscle ; Two-filament system ; Small intestine ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The structure of glycerinated smooth muscle from small intestine of adult rat was investigated by electron microscopy. In the central parts of the tissue blocks a two-filament system was found, consisting of parallel thick and thin filaments with regularly spaced interconnections, closely resembling that of striated muscle. In the peripheral parts of the blocks only thin filaments were found. The thin filaments were identified as actin by the formation of arrowhead complexes after incubation with heavy meromyosin.
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    URL: http://dx.doi.org/10.1007/BF00935994
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    Identification of helical polyribosomes in sections of mature skeletal muscle fibers (1971)
    Springer
    Cell & tissue research 121 (1971), S. 531-547 
    Details
    ISSN: 1432-0878
    Keywords: Helical polyribosomes ; Skeletal muscle ; Polysomes ; Levator ani ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The localization and configurations of ribosomes in mature white skeletal muscle fibers of the rat were investigated. Differential visualization of ribosomes and glycogen granules was obtained by fixation with glutaraldehyde only and staining of the sections with uranyl acetate. Ribosomes are then electron dense and glycogen granules electron transparent. Their identity was ascertained by selective extractions of ribonucleic acid and polysaccharide. The vast majority of the ribosomes is not membrane-bound. They are located intermyofibrillarly (predominantly at the level of the I-bands), beneath the sarcolemma, and in the paranuclear cones of sarcoplasm. Occasionally short stretches of granular reticulum occur, often as characteristic double walled vesicles with ribosomes on the inner membrane only. Three main types of polysomal configurations are observed: rosettes of 4 to 6 ribosomes, helical arrays, and whorls of up to about 25 probably membrane-bound ribosomes. The average number of ribosomes in the extended helical configurations is estimated to be about 60. It is argued that these helices represent the polysomes instrumental in the synthesis of the large subunits of myosin. It is emphasized that helical polyribosomes are by no means typical of striated muscle, but probably represent a common configuration of large free polysomes.
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    URL: http://dx.doi.org/10.1007/BF00560158
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    Licht- und elektronenmikroskopische Untersuchungen über die Reversibilität von Kernmembranschrumpfungen des Ependyms im Hypothalamus der Ratte, hervorgerufen durch langdauernde Thiobarbiturat-Narkose (1971)
    Springer
    Cell & tissue research 112 (1971), S. 542-550 
    Details
    ISSN: 1432-0878
    Keywords: Ependyma ; Thiobarbiturate narcosis ; Cell nucleus shrinkage ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Im Ependym des Hypothalamus wurde das Verhalten der Zellkern-Membran nach ausgedehnter intraperitonealer Thiobarbiturat-Narkose untersucht. Die Kernmembran ist gefaltet, der Zellkern zeigt Zeichen von Schrumpfung. Diese Veränderung ist reversibel und wird nicht von der mit der Narkose verbundenen Entwässerung verursacht. Elektronenmikroskopische Untersuchungen lassen einen normalen Stoffwechsel vermuten, Zeichen von Pyknose fehlen.
    Notes: Summary Effects on the nuclear membrane were investigated in hypothalamic ependymal cells of the rat during prolonged intraperitoneal thiobarbiturate narcosis. The nuclear membrane of the nucleus was folded and the nucleus showed signs of shrinkage. These alterations were reversible and did not depend on the dehydration which developed during the narcosis. Ultrastructural signs and the absence of pyknosis suggested that metabolism during nuclear shrinkage may be normal.
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    URL: http://dx.doi.org/10.1007/BF01033379
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    Ultrastructural observations on the postnatal development of the rat prostate (1971)
    Springer
    Cell & tissue research 113 (1971), S. 157-173 
    Details
    ISSN: 1432-0878
    Keywords: Prostate ; Development ; Ultrastructure ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The postnatal development of the rat prostate has been studied with the electron microscope. Major developmental changes begin during the second week after birth and involve organelles associated with the formation of secretions. The amount of granular endoplasmic reticulum and the size of the Golgi complex increase greatly. Large vacuoles that probably contain secretory material are formed, and the lumen of the prostatic acini appears to contain secreted material. Large lysosomes with polymorphic interiors are present as early as 10 days after birth, and they become numerous by the end of the third week. Differences in fine structure between the different lobes of the prostate are detectable in 10–14 day old rats. The subsequent differentiation of the granular endoplasmic reticulum into the forms characteristic of the different prostatic lobes is described. The initial changes in the prostate occur in advance of sexual maturity of the animal, and the adult appearance of the gland is attained by 4–5 weeks after birth.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00339413
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    An ultrastructural study of the neurosecretory cells of the medial vascular prechiasmatic gland, the preoptic recess and the anterior part of the suprachiasmatic area (1971)
    Springer
    Cell & tissue research 114 (1971), S. 404-440 
    Details
    ISSN: 1432-0878
    Keywords: Neurosecretory cells ; Rat ; Cytoplasmic bodies resembling nucleoli ; Paranucleolar and coiled bodies
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A distinctive cytoplasmic inclusion, termed nucleolus-resembling body, was identified in the neurons, but not in the glial cells, of the medial vascular prechiasmatic gland, the preoptic recess and the anterior part of the suprachiasmatic area in the rat. This ultrastructure which lacks a limiting membrane is made of an entanglement of tightly packed filaments, 60–70 Å in diameter. Each filament consists of smaller units, 15–20 Å thick. Usually, only one such ultrastructure is present in a cell, but at time two were identified. It is present in most neurosecretory cells. It is surrounded by polyribosomes in the perikaryon of the nerve cells. Furthermore, it is linked to peripherally located ribosomes by means of filaments, 70 Å in diameter. A possible function in protein synthesis of structural components, such as neurofilaments, is hypothesized. Two distinctive fibrillar areas were identified in the nucleolus according to the tightness with which fibrils were packed together. Their significance is discussed. Nucleolar extrusions were never observed in our studies. Paranucleolar and coiled bodies were identified in the nuclear sap. The mechanism of formation and the significance of these bodies are discussed.
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    URL: http://dx.doi.org/10.1007/BF00331464
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  • 95
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    Electronic Resource
    Long-term trophic effect of ACTH on rat adrenocortical cells (1971)
    Springer
    Cell & tissue research 115 (1971), S. 30-45 
    Details
    ISSN: 1432-0878
    Keywords: Adrenal Cortex ; ACTH ; Autoradiography ; Stereology ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The changes occurring in rat adrenocortical cells (zona fasciculata) during an 8 day period of treatment with ACTH, were investigated by morphometric and autoradiographic methods. The most important ultrastructural change consists in a conspicuous increase in the smooth endoplasmic reticulum, that accounts for about 50% of the total increase of cellular volume. Also the mitochondrial fraction shows a significant increase, which is found to be due both to the increment in the number of mitochondria per cell and to the increase in the mean volume of organelles themselves. The quantitative autoradiographic data, indicating an increment in the incorporation of 3H-orotate and 3H-leucine into adrenocortical cells of the treated animals, allow us to conclude that the ACTH-induced ultrastructural changes are the morphological expression of a stimulation of the cellular protein synthesis. Since mitochondria are largely autonomous in the synthesis of their enzymes and structural proteins, it is possible to hypothesize that ACTH also intervenes in the regulation of the mitochondrial protein synthesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00330212
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  • 96
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    Sur l'existence de cellules de type ≪corticotrope≫ dans la pars intermedia de l'hypophyse du rat (1971)
    Springer
    Cell & tissue research 115 (1971), S. 60-68 
    Details
    ISSN: 1432-0878
    Keywords: Adenohypophysis ; Pars intermedia ; Corticotrophic cells ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Résumé Nos observations démontrent l'existence de deux types de cellules glandulaires dans la pars intermedia de l'hypophyse du rat: des cellules propres, à M.S.H., dont les grains de sécrétion sont détruits par la fixation osmiée et relativement bien conservés par la fixation au glutaraldéhyde; des cellules à petits grains denses, osmio-résistants, qui s'apparentent morphologiquement aux éléments corticotropes de l'antéhypophyse. L'existence bien connue d'A.C.T.H. ou, plus précisément, d'un facteur ≪A.C.T.H.-like≫ dans le lobe neuro-intermédiaire nous fait considérer comme très vraisemblable la nature corticotrope de ce deuxième type cellulaire de la pars intermedia.
    Notes: Summary Two types of glandular cells have been shown to occur in the intermediate lobe of the rat hypophysis: 1) M.S.H. producing cells whose secretory granules are destroyed by osmium fixation and relatively well preserved by glutaraldehyde fixation, 2) cells with small dense osmium resistant granules being morphologically similar to the corticotrophic cells of the anterior lobe. The well known occurrence of A.C.T.H. or, more precisely, of an “A.C.T.H.-like” factor in the neuro-intermediate lobe makes the corticotrophic nature of this second cell type very likely.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00330214
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  • 97
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    Electronic Resource
    Neurofilaments and microtubules in sensory neurons after peripheral nerve section (1971)
    Springer
    Cell & tissue research 117 (1971), S. 191-211 
    Details
    ISSN: 1432-0878
    Keywords: Sensory neurons ; Rat ; Nerve section ; Neurofilaments ; Neurofibrils
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Sensory neurons were examined in spinal ganglia of the rat 1 to 55 days after section of the plexus brachialis nerves. Only “light” neurons of the type A were investigated. Maximal reaction to axotomy was found 7 to 14 days after the operation. The majority of the axotomized perikarya developed central chromatolysis. In such neurons, Nissl bodies virtually disappeared from the central area of the neuron and formed a more or less continuous zone at the cell circumference. The cytocentrum became filled with large numbers of mitochondria, dense bodies and other organelles. Neurofilaments and microtubules were disarranged and ran at random among the accumulated particles. Microtubules were often more prominent in chromatolytic areas than neurofilaments. Both these organelles were rare in the peripheral areas filled with massed Nissl substance. Part of the neurons that did not show typical chromatolysis contained increased numbers of neurofilaments among Nissl bodies dispersed throughout the cytoplasm. Neurofilaments were roughly arrayed in bundles up to several microns wide; they were linked by cross-bridges and separated by distances of about 500 Å. Microtubules were rarely found in the filamentous areas. However, they were numerous in the axon hillock and in the initial segment where they formed fascicles similar to those described in normal neurons of other types. During the period from 14 to 55 days after axotomy, many perikarya recovering from chromatolysis contained enlarged bundles of neurofilaments with occasional microtubules among the restored Nissl bodies. Mean diameters of sensory neurons, measured 7 to 55 days after axotomy, in no instance exceeded those of contralateral control neurons. It thus appears that sensory perikarya do not increase in size either during the chromatolytic process or during the period of recovery.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00330737
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  • 98
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    Electronic Resource
    Geschlechtsunterschiede in der Ultrastruktur der Nebennierenrinde der Ratte (1971)
    Springer
    Cell & tissue research 116 (1971), S. 136-150 
    Details
    ISSN: 1432-0878
    Keywords: Adrenal cortex ; Rat ; Sex difference ; ACTH ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Die Nebennierenrinde von 178 Sprague-Dawley-Ratten wurde elektronenmikroskopisch von der 1.–12. Lebenswoche unter Normalbedingungen sowie nach s.c. Injektion von 3 IE ACTH/100 g Körpergewicht innerhalb von 2 min — 24 Std untersucht. Ab der 5. Lebenswoche unterscheiden sich die Nebennieren von Weibchen im Bereich der äußeren Zona fasciculata durch Kernvergrößerung, größere Mitochondrien und kleindisperse Liposomen von denen der Männchen. Zyklusbedingte Schwankungen in der Ultrastruktur der weiblichen Nebennierenrinde bestehen nicht. Nach ACTH-Applikation nähert sich das Nebennierenbild des Männchen innerhalb von 30 min dem des unbehandelten Weibchens. Außerdem kommt es bei beiden Geschlechtern durch ACTH zu einer Dispersion und Reduktion der Liposomen, Vergrößerung des Golgiapparates und der Zellkerne, Ausweitung des endoplasmatischen Retikulums und Vermehrung der Mikrovilli. Der Geschlechtsdimorphismus wird funktionell erklärt.
    Notes: Summary 178 Sprague-Dawley-rats were studied by electron microscopy from the 1st to the 12th week of life under normal conditions and after s. c. injection of 3 I. U. ACTH/100 gbodyweight from 2 min to 24 hours. Beginning with the 5th week of life females differ from males by small dispersed liposomes and enlarged nuclei and mitochondria in the outer z. fasciculata. There is no change in the ultrastructure of the female adrenal cortex depending on the sexual cycle. After application of ACTH the adrenal cortex of males assimilates to the untreated females within 30 min. After ACTH-application both sexes show dispersion and reduction of liposomes, dilation of endoplasmatic reticulum, increasement of microvilli and enlargement of the Golgi apparatus. The sex-dimorphism is interpreted by functional differences.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00332862
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  • 99
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    Electronic Resource
    Wachstumsverhältnisse des Samenblasenepithels bei der heranwachsenden weißen Ratte (1971)
    Springer
    Cell & tissue research 119 (1971), S. 378-383 
    Details
    ISSN: 1432-0878
    Keywords: Seminal vesicles ; Rat ; Epithelium ; Proliferation activity ; Autoradiography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung An 12 männlichen weißen Wistarratten unterschiedlichen Alters wurde autoradiographisch die Proliferation des Samenblasenepithels zur Zeit der Pubertät geprüft. Die Tiere waren 21, 29, 40, 50, 60 und 180 Tage alt und erhielten 1 Std vor der Tötung durch Dekapitation 2,5 μC 3H-Thymidin/g Körpergewicht i. p. appliziert. Ausgewertet wurden Autoradiogramme nach einer Expositionszeit von 18 Tagen; bestimmt wurde der Prozentsatz der markierten Zellkerne (3H-Index). Im Gegensatz zu dem bekannten exponentiellen Abfall des 3H-Indexes bei nicht-hormonabhängigen Leberepithelien jugendlicher Ratten kommt es beim Epithel der Samenblasen um den 40. Lebenstag zu einem erneuten, starken Proliferationsschub. Diese starke Wachstumszunahme ist wahrscheinlich der proliferationskinetische Ausdruck der nach Steinberger (1970) zur gleichen Zeit stattfindenden hormonellen Umschaltung in den Testes. Anschließend geht das Vermehrungswachstum des jugendlichen Tieres, durch das die Zellzahl erhöht wird, in das Erhaltungswachstum über; der 3H-Index des Samenblasenepithels erreicht den Wert des erwachsenen Tieres.
    Notes: Summary In 12 male white rats (Wistar) the proliferative activity of the epithelial cells in the seminal vesicles was studied by autoradiography. The animals were 21, 29, 40, 50, 60 and 180 days old. Each animal was given 2.5 μC/g 3H-thymidine and was killed 1 hour thereafter. We analysed the autoradiograms recording the percentage of labelled nuclei (3H-index). While the 3H-index of the non hormone-dependent epithelial cells in the liver of young rats declines continously with growing age (Stocker et al., 1964), the epithelial cells in the seminal vesicles of 40 days old rats show a remarkable rise in their proliferative activity. It is quite possible that this new peak in the proliferation is the morphological result of a preceeding switch in hormonal production of the testes as reported by Steinberger (1970). Thereafter the growth proliferation of the young rats declines into the steady state of the grown up.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00431294
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  • 100
    Electronic Resource
    Electronic Resource
    Untersuchungen zur Fettresorption im Dünndarm der Ratte, unter besonderer Berücksichtigung der Tagesrhythmik (1971)
    Springer
    Cell & tissue research 119 (1971), S. 334-354 
    Details
    ISSN: 1432-0878
    Keywords: Fat absorption ; Small intestine ; Rat ; Micropinocytosis ; Circadian rhythm
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Die vorliegende Untersuchung will klären, welche Rolle die Mikropinocytose bei der Fettresorption spielt und ob die Fettresorption tagesrhythmische Schwankungen aufweist. Zu diesem Zweck wurde Fett zu verschiedenen Tages- und Nachtzeiten in abgebundene Jejunumabschnitte von Ratten injiziert, das Darmgewebe in verschiedenen Abständen licht- und elektronenmikroskopisch untersucht und die Zahl der mikropinocytotischen Invaginationen der Enterocyten bestimmt. Folgende Resultate wurden erzielt: Die Häufigkeit der Mikropinocytose (mikropinocytotische Invaginationen/100 Mikrovilli) beträgt bei Hungertieren 4,78, bei Normaltieren 5,06, 1 min nach Gabe von Mazolachymus 3,55, 1/16 m NaCl-Lösung 4,37, Butterschmalz 5,50, Rindertalg 7,16, Schweineschmalz 7,66 und Mazola 9,25; 2 1/2 min nach Mazolagabe 8,23, nach 5 min 7,67, nach 10 min 6,76. Zwischen 10 und 15 min wird der Ausgangswert erreicht. Aufgrund statistischer Auswertung der Befunde wird geschlossen, daß Fett vor allem in den Frühstadien der Fettresorption durch Mikropinocytose aufgenommen werden könnte. Ferner zeigt sich, daß die Mikropinocytosehäufigkeit entscheidend von der chemischen Zusammensetzung und dem physikalischchemischen Zustand der verabreichten Fette beeinflußt werden dürfte. — Lichtmikroskopisch wurde nachgewiesen, daß die Fettresorption charakteristische, tagesrhythmische Schwankungen aufweist. Um 2.00 Uhr ist sie am stärksten, um 14.00 Uhr am geringsten. Es wird diskutiert, worauf die tagesrhythmischen Unterschiede zurückzuführen sind.
    Notes: Summary The present study attempts to elucidate (a) to what extent micropinocytosis is involved in the process of fat absorption and (b) whether fat absorption shows a circadian rhythm. For this purpose rats were given 0.1 to 0.15 ml of fat directly into the upper jejunum at various times of the day and night. 20 sec to 20 min after administration of fat small pieces of the jejunum were rapidly dissected out, the tissue examined by means of light- and electron microscopy, and the number of micropinocytotic pits occurring in the enterocytes during fat absorption was determined. The following results were obtained: The frequency of micropinocytosis (micropinocytotic pits/100 microvilli) is as follows: in starved rats 4.78, in normal rats 5.06, 1 min after administration of corn oil chyme 3.55, 1/16 m NaCl-solution 4.37, clarified butter 5.50, ox tallow 7.16, lard 7.66, corn oil 9.25; 2 1/2 min after administration of corn oil 8.23, after 5 min 7.67, and after 10 min 6.76. Between 10 and 15 min the number of micropinocytotic pits reverses to normal. From statistical analysis of the results it is concluded that micropinocytosis can play a significant role in the early stages of fat absorption. The experiments show in addition that micropinocytosis is greatly influenced by the chemical composition and the physico-chemical state of the administered fat.—Light microscopic studies indicate that fat absorption varies considerably during the 24 hr period. The highest absorption was found at 2 a.m., the lowest at 2 p.m.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00431292
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